Multidrug resistance protein 1-like protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021731 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 30Note: Hover over icons to view figure legend
Homology
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Human
Match: ABCB1 (ATP binding cassette subfamily B member 1 [Source:HGNC Symbol;Acc:HGNC:40]) HSP 1 Score: 562.377 bits (1448), Expect = 8.937e-170 Identity = 299/615 (48.62%), Postives = 403/615 (65.53%), Query Frame = 1
Query: 304 VGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
V +F Y+ D + M+ + +I G PL++LVFG T A +L +++ IT+ + I D F + N+ M+ + ++GI + + +Y+ V+F+ + + QI IR FF A+MRQ IGWFD + G L +L DD++ ++ GIGDKIG Q A F G I+ F RGWKLTLV L+ISP++ + + +K + T K AY KAG +AEEVL +IRT+++F G+K E+ RY +NL+EA +IGI+K+I +G L++ SYAL FWYG +L+ G + +G +L VFFS L+ +GQA+P + +F AR AA EIF+IID P ID ++ G K ++ +G + F+ V F+YP+R + IL GL+L +Q GQTVALVG SGCGKS+ +QLMQR YD EG V +D +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP+K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK +GRTTI++AHRLSTVRNA VI G DG + E+G H ELM G+Y LV Q
Sbjct: 36 VFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFAN----AGNLEDLMSN----ITNRSDINDT---------GFFM---NLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGE-YSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQ 629 HSP 2 Score: 553.518 bits (1425), Expect = 1.252e-166 Identity = 271/583 (46.48%), Postives = 408/583 (69.98%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA---NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F+++M LN E Y +G AI NG +QP+F+I+F ++ +P ++Q++++ + + + +++F++ LQ FG +GE LTKR R + F +ML+QD++WFD+P N GALTTRL+++A Q++G G L + I ++G + ISF + WQ++L++LA VPII + G+++++++ G + DK LE SGKIA E IEN RTV +LT+E F Y ++++ +KAH G + TQA ++ +YA F YL+ + + +V + F A++FGAM +G+ + AP++ K KI+AAH+ ++++ P I+S+ST+G++ + + G++ V F+YP RP+I +L +S + QT+ALVG SGCGKST++ LL+RFYD L G +L+D +IK +N+Q LR IVSQEP LF SI ENIAYGD++++ + ++I+ AAK +NIH FI +LP++Y TKVG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+VF++G++ E GTH +LL++ GIY M+S+Q+
Sbjct: 697 FWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNL--FSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPN--TLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQA 1275 HSP 3 Score: 418.313 bits (1074), Expect = 7.902e-121 Identity = 235/540 (43.52%), Postives = 333/540 (61.67%), Query Frame = 1
Query: 568 SSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRR 2175
+SN+F+L+ F + + FI ++ F + +R FR+++RQ++ WFD +GALT +L +D V IG ++ + Q A GII++F+ GW+LTL+ L+I P+I I ++ K + K + +G IA E + + RT+VS E+ Y ++L + +RK+ + G +M+ SYA F +GA L+ D+L+VF + + +GQ + + +A+ +AA I II++ P ID + EG N +G +TF V F YPTR D P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G+VL+D IK LNV WLR +G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI +LPNK T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I ++G+V+E GTH +L+A G+Y +V Q TKR+
Sbjct: 751 NSNLFSLL------FLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAH-KLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAG---TKRQ 1280 HSP 4 Score: 371.318 bits (952), Expect = 3.196e-105 Identity = 222/595 (37.31%), Postives = 344/595 (57.82%), Query Frame = 2
Query: 2327 NAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANP--------NIEQQS------------ADIRTYVIALAGIAVVAFVSSVLQNVGFG--NSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
N +K Y+ +G ++AI +GA P ++FG + AN NI +S D+ Y +GI V++ +Q V F +G Q+ K R F A+++Q+I WFD +++G L TRL+D+ ++I G+ +G ++ + + F W+++LV+LA P++ + + +I+ F++ + A ++G +A E + IRTV + ++ Y + +++ KKA G I+ +YA F L+L + V F +++ GA ++G++ F + AA +F+++D P I+S+S G D N+ G+++ NV FSYP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD G + +D I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA++GRT +VIAHRLST++NAD I F DG I E G H EL+ + GIY +++++Q+
Sbjct: 44 NWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQ-VSFWCLAAGRQIHK-IRKQFFHAIMRQEIGWFD--VHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPD--NIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGREN--VTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQT 630
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Human
Match: ABCB1 (ATP binding cassette subfamily B member 1 [Source:HGNC Symbol;Acc:HGNC:40]) HSP 1 Score: 562.377 bits (1448), Expect = 8.937e-170 Identity = 299/615 (48.62%), Postives = 403/615 (65.53%), Query Frame = 1
Query: 304 VGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
V +F Y+ D + M+ + +I G PL++LVFG T A +L +++ IT+ + I D F + N+ M+ + ++GI + + +Y+ V+F+ + + QI IR FF A+MRQ IGWFD + G L +L DD++ ++ GIGDKIG Q A F G I+ F RGWKLTLV L+ISP++ + + +K + T K AY KAG +AEEVL +IRT+++F G+K E+ RY +NL+EA +IGI+K+I +G L++ SYAL FWYG +L+ G + +G +L VFFS L+ +GQA+P + +F AR AA EIF+IID P ID ++ G K ++ +G + F+ V F+YP+R + IL GL+L +Q GQTVALVG SGCGKS+ +QLMQR YD EG V +D +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP+K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK +GRTTI++AHRLSTVRNA VI G DG + E+G H ELM G+Y LV Q
Sbjct: 36 VFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFAN----AGNLEDLMSN----ITNRSDINDT---------GFFM---NLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGE-YSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQ 629 HSP 2 Score: 553.518 bits (1425), Expect = 1.252e-166 Identity = 271/583 (46.48%), Postives = 408/583 (69.98%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA---NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F+++M LN E Y +G AI NG +QP+F+I+F ++ +P ++Q++++ + + + +++F++ LQ FG +GE LTKR R + F +ML+QD++WFD+P N GALTTRL+++A Q++G G L + I ++G + ISF + WQ++L++LA VPII + G+++++++ G + DK LE SGKIA E IEN RTV +LT+E F Y ++++ +KAH G + TQA ++ +YA F YL+ + + +V + F A++FGAM +G+ + AP++ K KI+AAH+ ++++ P I+S+ST+G++ + + G++ V F+YP RP+I +L +S + QT+ALVG SGCGKST++ LL+RFYD L G +L+D +IK +N+Q LR IVSQEP LF SI ENIAYGD++++ + ++I+ AAK +NIH FI +LP++Y TKVG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+VF++G++ E GTH +LL++ GIY M+S+Q+
Sbjct: 697 FWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNL--FSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPN--TLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQA 1275 HSP 3 Score: 418.313 bits (1074), Expect = 7.902e-121 Identity = 235/540 (43.52%), Postives = 333/540 (61.67%), Query Frame = 1
Query: 568 SSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRR 2175
+SN+F+L+ F + + FI ++ F + +R FR+++RQ++ WFD +GALT +L +D V IG ++ + Q A GII++F+ GW+LTL+ L+I P+I I ++ K + K + +G IA E + + RT+VS E+ Y ++L + +RK+ + G +M+ SYA F +GA L+ D+L+VF + + +GQ + + +A+ +AA I II++ P ID + EG N +G +TF V F YPTR D P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G+VL+D IK LNV WLR +G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI +LPNK T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I ++G+V+E GTH +L+A G+Y +V Q TKR+
Sbjct: 751 NSNLFSLL------FLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAH-KLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAG---TKRQ 1280 HSP 4 Score: 371.318 bits (952), Expect = 3.196e-105 Identity = 222/595 (37.31%), Postives = 344/595 (57.82%), Query Frame = 2
Query: 2327 NAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANP--------NIEQQS------------ADIRTYVIALAGIAVVAFVSSVLQNVGFG--NSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
N +K Y+ +G ++AI +GA P ++FG + AN NI +S D+ Y +GI V++ +Q V F +G Q+ K R F A+++Q+I WFD +++G L TRL+D+ ++I G+ +G ++ + + F W+++LV+LA P++ + + +I+ F++ + A ++G +A E + IRTV + ++ Y + +++ KKA G I+ +YA F L+L + V F +++ GA ++G++ F + AA +F+++D P I+S+S G D N+ G+++ NV FSYP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD G + +D I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA++GRT +VIAHRLST++NAD I F DG I E G H EL+ + GIY +++++Q+
Sbjct: 44 NWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQ-VSFWCLAAGRQIHK-IRKQFFHAIMRQEIGWFD--VHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPD--NIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGREN--VTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQT 630
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Human
Match: ABCB1 (ATP binding cassette subfamily B member 1 [Source:HGNC Symbol;Acc:HGNC:40]) HSP 1 Score: 553.518 bits (1425), Expect = 2.283e-167 Identity = 271/583 (46.48%), Postives = 408/583 (69.98%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA---NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F+++M LN E Y +G AI NG +QP+F+I+F ++ +P ++Q++++ + + + +++F++ LQ FG +GE LTKR R + F +ML+QD++WFD+P N GALTTRL+++A Q++G G L + I ++G + ISF + WQ++L++LA VPII + G+++++++ G + DK LE SGKIA E IEN RTV +LT+E F Y ++++ +KAH G + TQA ++ +YA F YL+ + + +V + F A++FGAM +G+ + AP++ K KI+AAH+ ++++ P I+S+ST+G++ + + G++ V F+YP RP+I +L +S + QT+ALVG SGCGKST++ LL+RFYD L G +L+D +IK +N+Q LR IVSQEP LF SI ENIAYGD++++ + ++I+ AAK +NIH FI +LP++Y TKVG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+VF++G++ E GTH +LL++ GIY M+S+Q+
Sbjct: 633 FWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNL--FSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPN--TLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQA 1211 HSP 2 Score: 494.967 bits (1273), Expect = 3.480e-147 Identity = 253/460 (55.00%), Postives = 327/460 (71.09%), Query Frame = 1
Query: 769 LTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
L + D++ ++ GIGDKIG Q A F G I+ F RGWKLTLV L+ISP++ + + +K + T K AY KAG +AEEVL +IRT+++F G+K E+ RY +NL+EA +IGI+K+I +G L++ SYAL FWYG +L+ G + +G +L VFFS L+ +GQA+P + +F AR AA EIF+IID P ID ++ G K ++ +G + F+ V F+YP+R + IL GL+L +Q GQTVALVG SGCGKS+ +QLMQR YD EG V +D +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP+K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK +GRTTI++AHRLSTVRNA VI G DG + E+G H ELM G+Y LV Q
Sbjct: 107 LEEDMTSDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGE-YSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQ 565 HSP 3 Score: 419.083 bits (1076), Expect = 1.644e-121 Identity = 235/540 (43.52%), Postives = 333/540 (61.67%), Query Frame = 1
Query: 568 SSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRR 2175
+SN+F+L+ F + + FI ++ F + +R FR+++RQ++ WFD +GALT +L +D V IG ++ + Q A GII++F+ GW+LTL+ L+I P+I I ++ K + K + +G IA E + + RT+VS E+ Y ++L + +RK+ + G +M+ SYA F +GA L+ D+L+VF + + +GQ + + +A+ +AA I II++ P ID + EG N +G +TF V F YPTR D P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G+VL+D IK LNV WLR +G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI +LPNK T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I ++G+V+E GTH +L+A G+Y +V Q TKR+
Sbjct: 687 NSNLFSLL------FLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAH-KLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAG---TKRQ 1216 HSP 4 Score: 327.791 bits (839), Expect = 2.967e-91 Identity = 203/574 (35.37%), Postives = 317/574 (55.23%), Query Frame = 2
Query: 2327 NAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANP-NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
N +K Y+ +G ++AI +GA P ++FG + AN N+E ++I S + + GF + E + D++ +E G+ G+ +G ++ + + F W+++LV+LA P++ + + +I+ F++ + A ++G +A E + IRTV + ++ Y + +++ KKA G I+ +YA F L+L + V F +++ GA ++G++ F + AA +F+++D P I+S+S G D N+ G+++ NV FSYP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD G + +D I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA++GRT +VIAHRLST++NAD I F DG I E G H EL+ + GIY +++++Q+
Sbjct: 44 NWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITN--------------RSDINDTGF------------FMNLEEDMTSDVSKINE--------------------GI-GDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPD--NIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGREN--VTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQT 566
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Human
Match: ABCB4 (ATP binding cassette subfamily B member 4 [Source:HGNC Symbol;Acc:HGNC:45]) HSP 1 Score: 554.288 bits (1427), Expect = 5.241e-167 Identity = 282/583 (48.37%), Postives = 406/583 (69.64%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPN---IEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F KV+ LN E Y +G V AI NG +QP+FS++F ++ A+ P ++QQ +I + + GI ++F + LQ FG +GE LT+R RS+ F+AML+QD++WFD+ N+ GAL+TRL+ +A Q++G +G L + I ++G + ISF + WQ++L++LA VPII V GI++++++ G + DK LE +GKIA E IENIRTV +LT+E F Y E + +KAH G + ++QA ++ +YA F YL++ + +V + F A++FGA+ LG + + AP++ K K++AAH+F L +R P I+S+S +G L + G+I V F+YP R N+ +L +S + QT+ALVG SGCGKST++ LL+RFYD L G +L+D + K +N+Q LR IVSQEP LF SI ENIAYGD++++ + D+I++AAKA+NIH FI LP +YET+VG+KG+ SGGQKQRIAIARAL+R P+ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+VF++G++ E GTH +LL++ GIY M+S+Q+
Sbjct: 697 FLKVLKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEII-AIFGPGDDAVKQQKCNIFSLIFLFLGI--ISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEG--LKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQA 1274 HSP 2 Score: 534.258 bits (1375), Expect = 4.836e-160 Identity = 289/616 (46.92%), Postives = 403/616 (65.42%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
GV LF Y+ D + M IM+I G PL+++VFG T + ++++ F + P+ S S+ + +I+ + M+ + ++G+ + + +Y+ V+F+ + + QI IR FF A++RQ IGWFD ++ L +L DDI+ + GIGDK+G Q A F G I+ F+RGWKLTLV ++ISP++ + + +K + + K AY KAG +AEE LG+IRT+++F G+ E+ RY ++L+ A +IGI+K+I +G+ L++ SYAL FWYG++L+ + +G+ + VFFS L+ +GQA P + +F AR AA IF+IID P+ID +E G K + +G + F V F+YP+R + IL GL+L +Q GQTVALVG+SGCGKS+ +QL+QR YD +EG + ID +I++ NV +LRE IGVVSQEPVLF +I ENI YGR +VT +EI +A+ ANA FI LP K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE VQ ALDK EGRTTI++AHRLSTVRNA VI G +DG + EQG+H+ELM G+Y LV+ Q
Sbjct: 41 GVLTLFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDK------FVDTAGNF----SFPVNFSLSLLNPGKILEEE--------------MTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKE-YTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQ 631 HSP 3 Score: 415.616 bits (1067), Expect = 6.089e-120 Identity = 220/488 (45.08%), Postives = 318/488 (65.16%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+R+ F+A++RQ++ WFD ++GAL+ +L D V G ++ + Q A GII++F+ GW+LTL+ L++ P+I + ++ K + ++ + AG IA E + +IRT+VS E+ + Y E L + ++K+ + G + M+ SYA F +GA LI G+ D+++VF + + +G A+ + +A+ +AA +F + +R P ID +EEG K + F+G ITF V F YPTR + P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G VL+D K LNV WLR Q+G+VSQEP+LF CSI ENI YG V+ +EI A AN FI LP+K +T VGD+GTQLSGGQKQRIAIARAL+R P+ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I ++G+V+E GTH +L+A G+Y +V Q
Sbjct: 787 LRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGH-MRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQ 1273 HSP 4 Score: 359.377 bits (921), Expect = 2.446e-101 Identity = 223/590 (37.80%), Postives = 346/590 (58.64%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG------------FLLP-----AMANPN--IEQQSADIRTYVIAL-AGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+K ++ LG + AI +G+ P I+FG F P ++ NP +E++ Y L AG+ V A++ + +G Q+ ++ R F A+L+Q+I WFD N+ L TRL+D+ ++I G+ +G A+ + A + F W+++LV++A PI+ + + +I+ FS+ + A ++G +A E + IRTV ++ ++ Y++ ++ KKA + G+ I+ +YA F L++ + N F +++ GA ++G++ F + AA +F+++D P I+SFS G D ++ G+++ +V FSYP R N+ IL ++ + S QT+ALVG SGCGKST + L+QR YD G I ID I++ N+ LR++ +VSQEP LFS +I ENI YG T D+I A K +N ++FI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE VQ ALDKA+EGRT +VIAHRLST++NAD I F+DG I E G+H EL+ K G+Y +++++Q+
Sbjct: 53 DKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLA---AGRQI-RKIRQKFFHAILRQEIGWFD--INDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPD--SIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGN--VTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQT 632
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Human
Match: ABCB4 (ATP binding cassette subfamily B member 4 [Source:HGNC Symbol;Acc:HGNC:45]) HSP 1 Score: 554.288 bits (1427), Expect = 5.241e-167 Identity = 282/583 (48.37%), Postives = 406/583 (69.64%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPN---IEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F KV+ LN E Y +G V AI NG +QP+FS++F ++ A+ P ++QQ +I + + GI ++F + LQ FG +GE LT+R RS+ F+AML+QD++WFD+ N+ GAL+TRL+ +A Q++G +G L + I ++G + ISF + WQ++L++LA VPII V GI++++++ G + DK LE +GKIA E IENIRTV +LT+E F Y E + +KAH G + ++QA ++ +YA F YL++ + +V + F A++FGA+ LG + + AP++ K K++AAH+F L +R P I+S+S +G L + G+I V F+YP R N+ +L +S + QT+ALVG SGCGKST++ LL+RFYD L G +L+D + K +N+Q LR IVSQEP LF SI ENIAYGD++++ + D+I++AAKA+NIH FI LP +YET+VG+KG+ SGGQKQRIAIARAL+R P+ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+VF++G++ E GTH +LL++ GIY M+S+Q+
Sbjct: 697 FLKVLKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEII-AIFGPGDDAVKQQKCNIFSLIFLFLGI--ISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEG--LKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQA 1274 HSP 2 Score: 534.258 bits (1375), Expect = 4.836e-160 Identity = 289/616 (46.92%), Postives = 403/616 (65.42%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
GV LF Y+ D + M IM+I G PL+++VFG T + ++++ F + P+ S S+ + +I+ + M+ + ++G+ + + +Y+ V+F+ + + QI IR FF A++RQ IGWFD ++ L +L DDI+ + GIGDK+G Q A F G I+ F+RGWKLTLV ++ISP++ + + +K + + K AY KAG +AEE LG+IRT+++F G+ E+ RY ++L+ A +IGI+K+I +G+ L++ SYAL FWYG++L+ + +G+ + VFFS L+ +GQA P + +F AR AA IF+IID P+ID +E G K + +G + F V F+YP+R + IL GL+L +Q GQTVALVG+SGCGKS+ +QL+QR YD +EG + ID +I++ NV +LRE IGVVSQEPVLF +I ENI YGR +VT +EI +A+ ANA FI LP K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE VQ ALDK EGRTTI++AHRLSTVRNA VI G +DG + EQG+H+ELM G+Y LV+ Q
Sbjct: 41 GVLTLFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDK------FVDTAGNF----SFPVNFSLSLLNPGKILEEE--------------MTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKE-YTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQ 631 HSP 3 Score: 415.616 bits (1067), Expect = 6.089e-120 Identity = 220/488 (45.08%), Postives = 318/488 (65.16%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+R+ F+A++RQ++ WFD ++GAL+ +L D V G ++ + Q A GII++F+ GW+LTL+ L++ P+I + ++ K + ++ + AG IA E + +IRT+VS E+ + Y E L + ++K+ + G + M+ SYA F +GA LI G+ D+++VF + + +G A+ + +A+ +AA +F + +R P ID +EEG K + F+G ITF V F YPTR + P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G VL+D K LNV WLR Q+G+VSQEP+LF CSI ENI YG V+ +EI A AN FI LP+K +T VGD+GTQLSGGQKQRIAIARAL+R P+ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I ++G+V+E GTH +L+A G+Y +V Q
Sbjct: 787 LRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGH-MRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQ 1273 HSP 4 Score: 359.377 bits (921), Expect = 2.446e-101 Identity = 223/590 (37.80%), Postives = 346/590 (58.64%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG------------FLLP-----AMANPN--IEQQSADIRTYVIAL-AGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+K ++ LG + AI +G+ P I+FG F P ++ NP +E++ Y L AG+ V A++ + +G Q+ ++ R F A+L+Q+I WFD N+ L TRL+D+ ++I G+ +G A+ + A + F W+++LV++A PI+ + + +I+ FS+ + A ++G +A E + IRTV ++ ++ Y++ ++ KKA + G+ I+ +YA F L++ + N F +++ GA ++G++ F + AA +F+++D P I+SFS G D ++ G+++ +V FSYP R N+ IL ++ + S QT+ALVG SGCGKST + L+QR YD G I ID I++ N+ LR++ +VSQEP LFS +I ENI YG T D+I A K +N ++FI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE VQ ALDKA+EGRT +VIAHRLST++NAD I F+DG I E G+H EL+ K G+Y +++++Q+
Sbjct: 53 DKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLA---AGRQI-RKIRQKFFHAILRQEIGWFD--INDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPD--SIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGN--VTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQT 632
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Celegans
Match: pgp-9 (p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EG58]) HSP 1 Score: 534.258 bits (1375), Expect = 2.928e-160 Identity = 286/631 (45.32%), Postives = 388/631 (61.49%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDM-EEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ-----KNRPWTKRRERR 2184
+L+ Y + D +M+ II+S GV PL+ ++ GN + T T FL + + + + E+++N ++ + +F ++ + F++ + + R FF +VMRQ I W+D SG L+NKL D++ V G GDK+G Q A FIGG +AF W LTL+ +S+SP ++IC L L+K + K ++ Y AGGIAEEVL SIRT+++F G++YE RY + L+ K GI+KS L G L +++ SY L FW G + + G G +L VFFS +M + +GQA + A AAA ++E+IDR+PEID + EG + G+I+ V+FTYPTR D IL G+SL QPGQTVALVG+SGCGKS++IQL+QRFY+ + G++LIDD I+D N+ +LR+ +GVVSQEP LF SI++NIRYGR DV+DE+I +AL ANA FI P L+TLVGDRG Q+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ AL+ GRTTI++AHRLSTVRNA I +K G+V E GTH L+ GLY +LV Q ++P K ERR
Sbjct: 33 QLYRYTSTVDRLMLAVGIIVSCATGVGLPLMSIIMGNVSQNFVTLG------TIFLDPNSTASEKAAARAEFSHEVIQN-------------------CLKYVYLGCGIFAAGFLQASCFMVICEKLSNRFRRQFFHSVMRQEIAWYDKNTSGTLSNKLFDNLERVREGTGDKVGLAFQMMAQFIGGFAVAFTYDWLLTLIMMSLSPFMMICGLFLAKLLATAATKEAKQYAVAGGIAEEVLTSIRTVIAFNGQEYECKRYEDALEHGKKTGIKKSFLIGAGLASFFVIIYASYCLAFWVGTNFVYSGR-LESGTVLTVFFSVMMGSMALGQAGQQFATIGTALGAAASLYEVIDRIPEIDAYSTEGQTPSKISGRISVNKVEFTYPTRADVKILKGVSLDAQPGQTVALVGSSGCGKSTIIQLLQRFYNPDAGQILIDDIPIEDFNIKYLRQLVGVVSQEPNLFNTSIEQNIRYGRSDVSDEDIARALKEANAADFIKTFPEGLNTLVGDRGVQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESESIVQSALENASRGRTTIVIAHRLSTVRNADKIIVMKAGQVMEVGTHETLIEQKGLYHELVHAQVFADVDDKPKKKEAERR 637 HSP 2 Score: 443.351 bits (1139), Expect = 1.983e-129 Identity = 247/585 (42.22%), Postives = 352/585 (60.17%), Query Frame = 2
Query: 2300 AGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYN---MSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
A FK++ PE YIF ++A+ GAV P+FS+ F ++ +NP+ +Q D + + +A V S + Q FG + E+LT R RS + +L+QD +FD P ++ G +TTRL+ +A I+ LG++ AI S+G + I+F + WQ++ +V+A P + VG L ++ G + SD +E +GK A+E IENIRTV LT + + + G KA +G YG + F YAA F G L+++ + + NV A+ F +G + + P + K AA +F +L+ P I+ ++ G +SG++K+ V F YP RP + IL ++ ++ QT+ALVGPSGCGKST+I LL+R YD L G + +DNN ++ +N ++LR+ ++VSQEP LF SIRENI YG +T +QI A +NIH FI LPD YET+VGEKG+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESEK VQ ALD A + RTC+V+AHRLSTI NA I+V K+G++ E GTH EL++K G Y + QS
Sbjct: 690 ANLFKILRYARPEWIYIFFAIIAALIQGAVMPAFSLFFSQIINVFSNPDRDQMKKDGHFWALMFLVLAAVQGTSMLFQCSLFGVAAERLTMRIRSKVYRNVLRQDATYFDMPKHSPGRITTRLATDAPNIKSAIDYRLGSIFNAIASVGGGLGIAFYYGWQMAFLVMAIFPFMAVGQALMMKYHGGSATSDAKEMENAGKTAMEAIENIRTVQALTLQTKLYNIFCSHLDAPHGGNISKAIIRGLTYGFANSIQFFTYAAAFRF-GLFLIFDKNVLMEPENVLRVLFAISFSFGTIGFAASYFPEYIKATFAAGLIFNMLEEEPRIDGMTSSGTY---PQLSGEVKLNKVFFRYPERPAVPILQGLNVHVKPGQTLALVGPSGCGKSTVISLLERLYDPLEGAVTVDNNDLRQMNPKHLRKHIALVSQEPILFDTSIRENIVYGLQPGEYTHEQIETACSKANIHKFIDELPDGYETRVGEKGTQLSGGQKQRIAIARALIRNPKILLLDEATSALDTESEKQVQVALDAAAKDRTCIVVAHRLSTIVNAGCIMVVKNGQVVEQGTHNELIAKRGAYFALTQKQS 1270 HSP 3 Score: 343.584 bits (880), Expect = 1.714e-96 Identity = 205/514 (39.88%), Postives = 309/514 (60.12%), Query Frame = 2
Query: 2513 FVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYL---LLYNMS-KMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F + LQ F E+L+ R R F ++++Q+IAW+D+ N G L+ +L D ++R +G+ +G + A++F + W ++L++++ P + + G+ +++ + + +G IA E + +IRTV + + Y++A++ KK+ FL G A+ F A++ LA ++ +Y+ + V F +++ G+M LG++ G AAA ++E++DR+P I+++ST+G +SG I + V+F+YP R ++ IL VS + QT+ALVG SGCGKSTII LLQRFY+ G ILID+ I+D NI+ LRQL +VSQEP LF+ SI +NI YG S +D+ I A K +N DFI P+ T VG++G SGGQKQRIAIARAL+RNP+ILLLDEATSALD ESE IVQ AL+ A GRT +VIAHRLST++NADKI+V K G++ E GTH L+ + G+Y +++ Q
Sbjct: 117 FAAGFLQASCFMVICEKLSNRFRRQFFHSVMRQEIAWYDK--NTSGTLSNKLFDNLERVREGTGDKVGLAFQMMAQFIGGFAVAFTYDWLLTLIMMSLSPFMMICGLFLAKLLATAATKEAKQYAVAGGIAEEVLTSIRTVIAFNGQEYECKRYEDALEHGKKTGIKKS----FLIGAGLASFFVIIYASYCLAFWVGTNFVYSGRLESGTVLTVFFSVMMGSMALGQAGQQFATIGTALGAAASLYEVIDRIPEIDAYSTEGQT--PSKISGRISVNKVEFTYPTRADVKILKGVSLDAQPGQTVALVGSSGCGKSTIIQLLQRFYNPDAGQILIDDIPIEDFNIKYLRQLVGVVSQEPNLFNTSIEQNIRYGRSD--VSDEDIARALKEANAADFIKTFPEGLNTLVGDRGVQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESESIVQSALENASRGRTTIVIAHRLSTVRNADKIIVMKAGQVMEVGTHETLIEQKGLYHELVHAQ 620 HSP 4 Score: 341.658 bits (875), Expect = 7.339e-96 Identity = 200/506 (39.53%), Postives = 297/506 (58.70%), Query Frame = 1
Query: 658 TFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTI--VSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGD-MLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRM--DVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKN 2154
+ F + ++ IR+ +R V+RQ+ +FD + G +T +L D + + I ++G + A GG+ +AF GW++ + ++I P + + ++ K ++ AG A E + +IRT+ ++ + + Y + + +LD I K+I+ G G + F +YA F +G LI + N + + +L V F+ + IG A + + +A AA IF +++ P ID M G G++ V F YP R PIL GL++ ++PGQT+ALVG SGCGKS++I L++R YD EG V +D+ +++ +N LR+ I +VSQEP+LF SI+ENI YG + T E+I+ A AN FI+ LP+ +T VG++GTQLSGGQKQRIAIARAL+RNPKILLLDEATSALD+ESEK VQ ALD + RT I+VAHRLST+ NA I +K+G+V EQGTH EL+A G Y L +Q +
Sbjct: 769 SLFGVAAERLTMRIRSKVYRNVLRQDATYFDMPKHSPGRITTRLATDAPNIKSAIDYRLGSIFNAIASVGGGLGIAFYYGWQMAFLVMAIFPFMAVGQALMMKYHGGSATSDAKEMENAGKTAMEAIENIRTVQALTLQTKLYNI--FCSHLDAPHGGNISKAIIRGLTYGFANSIQFFTYAAAFRFGLFLIFDKNVLMEPENVLRVLFAISFSFGTIGFAASYFPEYIKATFAAGLIFNMLEEEPRIDGMTSSG-TYPQLSGEVKLNKVFFRYPERPAVPILQGLNVHVKPGQTLALVGPSGCGKSTVISLLERLYDPLEGAVTVDNNDLRQMNPKHLRKHIALVSQEPILFDTSIRENIVYGLQPGEYTHEQIETACSKANIHKFIDELPDGYETRVGEKGTQLSGGQKQRIAIARALIRNPKILLLDEATSALDTESEKQVQVALDAAAKDRTCIVVAHRLSTIVNAGCIMVVKNGQVVEQGTHNELIAKRGAYFALTQKQSS 1271
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Celegans
Match: pgp-1 (Multidrug resistance protein pgp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34712]) HSP 1 Score: 491.115 bits (1263), Expect = 2.693e-145 Identity = 265/617 (42.95%), Postives = 381/617 (61.75%), Query Frame = 1
Query: 304 VGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSL-TQFL-TSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+ +L+ Y T + +++ ++++ G PL+ ++ G + Q +N+ + FL T QN T D E V N +++ A + + M+ ++VT ++ ++ + +R F ++++RQ I WFD +SG L KL D++ V G GDKIG Q+ + FI G I+AF W+LTLV L+++P+ +C ++K+M + + Y KAG + EE + SIRT+VS G +YE+ RY+ ++EA K G+ K + G G + F S+AL F+ G + +G+ GDML F S +M + +G A P + A+ AA+ I+E++DR P ID ++ G K +G IT + V FTYP+R D PIL G++L + GQTVALVG+SGCGKS++I L+ R+YD +G++ ID +++D+N+ +LR+ + VVSQEP LF C+I+ENI G+ +T EE+ A MANA+ FI LPN +TLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDK +GRTTII+AHRLST+RNA +I K+G+V E G H LMA GLY DLV Q
Sbjct: 62 IPQLYRYTTTLEKLLLFIGTLVAVITGAGLPLMSILQGKVSQAFINEQIVINNNGSTFLPTGQNYTKT------DFEHDVMNVVWSY------------------AAMTVGMWAAGQITVTCYLYVAEQMNNRLRREFVKSILRQEISWFDTNHSGTLATKLFDNLERVKEGTGDKIGMAFQYLSQFITGFIVAFTHSWQLTLVMLAVTPIQALCGFAIAKSMSTFAIRETLRYAKAGKVVEETISSIRTVVSLNGLRYELERYSTAVEEAKKAGVLKGLFLGISFGAMQASNFISFALAFYIGVGWVHDGS-LNFGDMLTTFSSVMMGSMALGLAGPQLAVLGTAQGAASGIYEVLDRKPVIDSSSKAGRKDMKIKGDITVENVHFTYPSRPDVPILRGMNLRVNAGQTVALVGSSGCGKSTIISLLLRYYDVLKGKITIDGVDVRDINLEFLRKNVAVVSQEPALFNCTIEENISLGKEGITREEMVAACKMANAEKFIKTLPNGYNTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDAESEGIVQQALDKAAKGRTTIIIAHRLSTIRNADLIISCKNGQVVEVGDHRALMAQQGLYYDLVTAQ 653 HSP 2 Score: 411.764 bits (1057), Expect = 9.201e-119 Identity = 235/586 (40.10%), Postives = 345/586 (58.87%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA-NPNIEQQSADIRT----YVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKM--TNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F+++ P +F+G +A G + P++S+ F + A NP AD + + + +A + S L G + E LT+ R+ F +L Q I +FD P N G ++TRL+ + +R VIT + SM A + ++F + WQ++L+++A +PI+ G L+ R G + +SGKIA+E IEN+RTV L RE TF +++ E + E K+A QG YG + ++ + + L++ + M V A+ LG + + P + K A +F +L ++ I+S S G + + + G + +NV+F+YP RP I IL +SF++ QT+ALVGPSGCGKST++ LL+RFYDTL G I ID ++IK +N ++ R +IVSQEPTLF SI ENI YG T Q+ AA+ +NIH+FI LP+ +ET+VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESEK+VQ+ALD+A+EGRTC+VIAHRL+T+ NAD I V +G I E GTH +L+S+ G Y ++ Q
Sbjct: 740 LFEILYHARPHALSLFIGMSTATIGGFIYPTYSVFFTSFMNVFAGNP------ADFLSQGHFWALMFLVLAAAQGICSFLMTFFMGIASESLTRDLRNKLFRNVLSQHIGFFDSPQNASGKISTRLATDVPNLRTAIDFRFSTVITTLVSMVAGIGLAFFYGWQMALLIIAILPIVAFGQYLRGRRFTGKNVKSASEFADSGKIAIEAIENVRTVQALAREDTFYENFCEKLDIPHKEAIKEAFIQGLSYGCASSVLYLLNTCAYRMGLALIITDPPTMQPMRVLRVMYAITISTSTLGFATSYFPEYAKATFAGGIIFGMLRKISKIDSLSLAG---EKKKLYGKVIFKNVRFAYPERPEIEILKGLSFSVEPGQTLALVGPSGCGKSTVVALLERFYDTLGGEIFIDGSEIKTLNPEHTRSQIAIVSQEPTLFDCSIAENIIYGLDPSSVTMAQVEEAARLANIHNFIAELPEGFETRVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEKVVQEALDRAREGRTCIVIAHRLNTVMNADCIAVVSNGTIIEKGTHTQLMSEKGAYYKLTQKQ 1316 HSP 3 Score: 370.163 bits (949), Expect = 4.737e-105 Identity = 216/537 (40.22%), Postives = 316/537 (58.85%), Query Frame = 1
Query: 562 LSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDA-QN-SGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLD----EACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
LS + + LM F +A A I S++ F I S+ +RN FR V+ Q+IG+FD+ QN SG ++ +L D+ + I + ++ + GI LAF GW++ L+ ++I P++ + + K++ + +G IA E + ++RT+ + E + E LD EA K + + G ++ L +Y +G A +I + +L V ++ ++T+ +G AT + + +A A IF ++ ++ +ID ++ G+K GK+ FK V+F YP R + IL GLS S++PGQT+ALVG SGCGKS+++ L++RFYD+ GE+ ID + IK LN R QI +VSQEP LF CSI ENI YG VT ++++A +AN FI LP +T VGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESEK+VQ+ALD+ EGRT I++AHRL+TV NA I + +G + E+GTH +LM+ G Y L +Q
Sbjct: 790 LSQGHFWALM------FLVLAAAQGICSFLMTFFMGIASESLTRDLRNKLFRNVLSQHIGFFDSPQNASGKISTRLATDVPNLRTAIDFRFSTVITTLVSMVAGIGLAFFYGWQMALLIIAILPIVAFGQYLRGRRFTGKNVKSASEFADSGKIAIEAIENVRTVQALAREDTFYENFCEKLDIPHKEAIKEAFIQGLSYGCASSVLYLLNTCAYRMGL---ALIITDPPTMQPMRVLRVMYAITISTSTLGFATSYFPEYAKATFAGGIIFGMLRKISKIDSLSLAGEK-KKLYGKVIFKNVRFAYPERPEIEILKGLSFSVEPGQTLALVGPSGCGKSTVVALLERFYDTLGGEIFIDGSEIKTLNPEHTRSQIAIVSQEPTLFDCSIAENIIYGLDPSSVTMAQVEEAARLANIHNFIAELPEGFETRVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEKVVQEALDRAREGRTCIVIAHRLNTVMNADCIAVVSNGTIIEKGTHTQLMSEKGAYYKLTQKQ 1316 HSP 4 Score: 359.762 bits (922), Expect = 1.539e-101 Identity = 223/601 (37.10%), Postives = 341/601 (56.74%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG------------------FLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK*IVQ*FKEGTY 4084
EK +F+G + A+ GA P SIL G LP N D+ V + A + V + + + + EQ+ R R +++L+Q+I+WFD +N+ G L T+L D +++ +G+ +G + ++F WQ++LV+LA PI + G + M F+ + ++GK+ E I +IRTV +L ++ Y A+++ K G +G QA+ F ++A F + + ++ +F +++ G+M LG + G + AA+ ++E+LDR P I+S S G D++ + GDI +ENV F+YP RP++ IL ++ +N+ QT+ALVG SGCGKSTII LL R+YD L G I ID ++DIN++ LR+ ++VSQEP LF+ +I ENI+ G T ++++AA K +N FI LP+ Y T VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD ESE IVQ ALDKA +GRT ++IAHRLSTI+NAD I+ K+G++ E G H L+++ G+Y +++ Q+ V EG +
Sbjct: 73 EKLLLFIGTLVAVITGAGLPLMSILQGKVSQAFINEQIVINNNGSTFLPTGQNYTKTDFEHDVMNVVWSYAAMTVGMWAAGQITVTCYLYVAEQMNNRLRREFVKSILRQEISWFD--TNHSGTLATKLFDNLERVKEGTGDKIGMAFQYLSQFITGFIVAFTHSWQLTLVMLAVTPIQALCGFAIAKSMSTFAIRETLRYAKAGKVVEETISSIRTVVSLNGLRYELERYSTAVEEAKKAGVLKGLFLGISFGAMQASNFISFALAFYIGVGWVHDGSLNFGDMLTTFSSVMMGSMALGLAGPQLAVLGTAQGAASGIYEVLDRKPVIDSSSKAGRK-DMK-IKGDITVENVHFTYPSRPDVPILRGMNLRVNAGQTVALVGSSGCGKSTIISLLLRYYDVLKGKITIDGVDVRDINLEFLRKNVAVVSQEPALFNCTIEENISLGKEG--ITREEMVAACKMANAEKFIKTLPNGYNTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDAESEGIVQQALDKAAKGRTTIIIAHRLSTIRNADLIISCKNGQVVEVGDHRALMAQQGLYYDLVTAQTFTDAVDSAAEGKF 667
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Celegans
Match: pgp-2 (ABC transporter PGP-2; p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EDF9]) HSP 1 Score: 470.7 bits (1210), Expect = 5.944e-139 Identity = 257/610 (42.13%), Postives = 367/610 (60.16%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIV--RNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDL 2136
+F Y D++++I + ++ G +PL+ +V G T T FL +QN + E +V ++N + I+ L+ G + MF SYV + F ++ + +R + +A++RQ I WFD Q +G LT +L DD+ V G+GDK ++Q +A F+ G + F W +TLV + +PLI++ +SK+M T+ E Y AG IAEE SIRT+ S G K E+ R+ L+ + GI K G +G M+ SYAL FWYG++LI F G + VFF+ L +T +G A PH+ SF AR AA+ + +I+ P+ID + EG +++ +G I+FK V F YP+R D +L G+SL ++ G +ALVG+SGCGKS+++ L+QRFYD +G VLID +++++NV LREQIG+VSQEPVLF +I ENI+ G T +++ +A MANA FI LP+ T VG++G QLSGGQKQRIAIARALV+NPKILLLDEATSALD+E+E+ VQ ALD+ GRTTIIVAHRLST+RN IF K G + E G+H ELM+ G++ D+
Sbjct: 38 IFYYTQGVDLLLLITGTVAAVIHGAGFPLLAIVLGGMT-------------TVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLG---------VLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDKQQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNALEVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVDNMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHATHDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQAQAGRTTIIVAHRLSTIRNVDRIFVFKAGNIVESGSHEELMSKQGIFYDM 625 HSP 2 Score: 422.165 bits (1084), Expect = 1.544e-122 Identity = 237/578 (41.00%), Postives = 358/578 (61.94%), Query Frame = 2
Query: 2309 FKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
FK+ N + + G A G+V P F++++ + + P +Q A++ + + + FV G GE LT + R F+ +L+QDIA++D+ + G L TR + +A +R V L V+ +I ++ A+ I F + WQ++L+++ VP++ +GG ++++ G D LEE+GK+A + +E+IRTV +L R+ F Y E +++ K AH G ++ +Q+ IF YAA F L + + +V+ F A+ F +G + + P+ K ++AA+ +F L++ PI+S S G+V + ++G+I I NV F+YP R + +L + +I + +T+ALVG SGCGKSTI+GLL+RFY+ G I+ID + I+++NI +LR+ IVSQEPTLF +I ENI YG + + T +I+ AAK +NIH+FI+ LPD Y+T VGEKG+ SGGQKQRIAIARAL+R+P +LLLDEATSALDTESEKIVQ+ALD A++GRTC+VIAHRLSTIQN+D I + +GKI E GTH EL+ K+ IY + Q
Sbjct: 688 FKIFKFNGDKVGWFIGGIFGAFIFGSVTPVFALVYAEIFNVYSLPA-DQMQANVYFWCGMFVLMGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRYVF-TRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQIRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFTYCEYLREPFNTNLKHAHTYGAVFAFSQSLIFFMYAAAFYLGSIFVNQQAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSGIV---KPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNV-TYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDAAKQGRTCLVIAHRLSTIQNSDVIAIVSEGKIVEKGTHDELIRKSEIYQKFCETQ 1259 HSP 3 Score: 369.392 bits (947), Expect = 4.720e-105 Identity = 217/584 (37.16%), Postives = 336/584 (57.53%), Query Frame = 2
Query: 2357 GCVSAIFNGAVQPSFSILFGFLLPAM--------------ANP------NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLY-----GLTQAAIFCAYAATFMLAGYLLLYNMS-KMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQM 4030
G V+A+ +GA P +I+ G + NP ++++ ++++ Y I + V+ F +S +Q F + E+L + R +A+L+Q I WFD+ G LT RL+D+ ++R G+ ++ + A + F + W ++LV++ F P+I + G + M + ++ +G IA E +IRTV +L + + A+ EV ++ + Y G + ++ +YA F L++ + + +F F A++ G+ +LG ++ +FG + AA+ + +++ P I+ +S +G+++D NM GDI ++V F YP R +I +L +S + + IALVG SGCGKSTI+ LLQRFYD G +LID ++++N+ +LR+ IVSQEP LF +I ENI G+ T DQ++ A K +N +DFI LPD Y T+VGEKG SGGQKQRIAIARAL++NP+ILLLDEATSALDTE+E+ VQ ALD+AQ GRT +++AHRLSTI+N D+I VFK G I ESG+H EL+SK GI+ M
Sbjct: 53 GTVAAVIHGAGFPLLAIVLGGMTTVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLGVLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDK--QQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNAL-----EVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVD--NMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHA--THDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQAQAGRTTIIVAHRLSTIRNVDRIFVFKAGNIVESGSHEELMSKQGIFYDM 625 HSP 4 Score: 332.413 bits (851), Expect = 6.408e-93 Identity = 200/540 (37.04%), Postives = 314/540 (58.15%), Query Frame = 1
Query: 574 NIFTLMSGMSQQFAGIALAMFIMSYVSVTFFV--ITSQHQIS--------AIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG-RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQK 2151
N+++L + Q MF++ + +TFFV TS + + +R F+ ++RQ+I ++D +G L + D V + ++ ++ G + + F GW+L L+ + + PL+++ + + ++ +AG +A + + IRT+ S R E++ Y E L E ++ + G + L+F YA F+ G+ + + D+ VFF+ IG T + +AR AA+ +F +I+ ID +++ G + G I+ + V F YPTR D +L G +L I+ G+TVALVG SGCGKS+++ L++RFY+ ++G ++ID NI++LN+ LREQ+ +VSQEP LF C+I ENI YG +VT +EI +A MAN FI LP+ DT VG++GTQLSGGQKQRIAIARALVR+P +LLLDEATSALD+ESEKIVQ+ALD +GRT +++AHRLST++N+ VI + +GK+ E+GTH EL+ + +Y+ + Q+
Sbjct: 727 NVYSLPADQMQANVYFWCGMFVL--MGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRY-VFTRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQIRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFT-YCEYLREPFNTNLKHAHTYGAVFAFSQSLIFFMYAAAFYLGSIFVNQ-QAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSG-IVKPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNVTYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDAAKQGRTCLVIAHRLSTIQNSDVIAIVSEGKIVEKGTHDELIRKSEIYQKFCETQR 1260
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Celegans
Match: pgp-2 (ABC transporter PGP-2; p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EDF9]) HSP 1 Score: 471.085 bits (1211), Expect = 6.656e-139 Identity = 257/610 (42.13%), Postives = 367/610 (60.16%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIV--RNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDL 2136
+F Y D++++I + ++ G +PL+ +V G T T FL +QN + E +V ++N + I+ L+ G + MF SYV + F ++ + +R + +A++RQ I WFD Q +G LT +L DD+ V G+GDK ++Q +A F+ G + F W +TLV + +PLI++ +SK+M T+ E Y AG IAEE SIRT+ S G K E+ R+ L+ + GI K G +G M+ SYAL FWYG++LI F G + VFF+ L +T +G A PH+ SF AR AA+ + +I+ P+ID + EG +++ +G I+FK V F YP+R D +L G+SL ++ G +ALVG+SGCGKS+++ L+QRFYD +G VLID +++++NV LREQIG+VSQEPVLF +I ENI+ G T +++ +A MANA FI LP+ T VG++G QLSGGQKQRIAIARALV+NPKILLLDEATSALD+E+E+ VQ ALD+ GRTTIIVAHRLST+RN IF K G + E G+H ELM+ G++ D+
Sbjct: 45 IFYYTQGVDLLLLITGTVAAVIHGAGFPLLAIVLGGMT-------------TVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLG---------VLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDKQQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNALEVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVDNMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHATHDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQAQAGRTTIIVAHRLSTIRNVDRIFVFKAGNIVESGSHEELMSKQGIFYDM 632 HSP 2 Score: 421.779 bits (1083), Expect = 2.007e-122 Identity = 237/578 (41.00%), Postives = 358/578 (61.94%), Query Frame = 2
Query: 2309 FKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
FK+ N + + G A G+V P F++++ + + P +Q A++ + + + FV G GE LT + R F+ +L+QDIA++D+ + G L TR + +A +R V L V+ +I ++ A+ I F + WQ++L+++ VP++ +GG ++++ G D LEE+GK+A + +E+IRTV +L R+ F Y E +++ K AH G ++ +Q+ IF YAA F L + + +V+ F A+ F +G + + P+ K ++AA+ +F L++ PI+S S G+V + ++G+I I NV F+YP R + +L + +I + +T+ALVG SGCGKSTI+GLL+RFY+ G I+ID + I+++NI +LR+ IVSQEPTLF +I ENI YG + + T +I+ AAK +NIH+FI+ LPD Y+T VGEKG+ SGGQKQRIAIARAL+R+P +LLLDEATSALDTESEKIVQ+ALD A++GRTC+VIAHRLSTIQN+D I + +GKI E GTH EL+ K+ IY + Q
Sbjct: 695 FKIFKFNGDKVGWFIGGIFGAFIFGSVTPVFALVYAEIFNVYSLPA-DQMQANVYFWCGMFVLMGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRYVF-TRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQIRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFTYCEYLREPFNTNLKHAHTYGAVFAFSQSLIFFMYAAAFYLGSIFVNQQAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSGIV---KPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNV-TYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDAAKQGRTCLVIAHRLSTIQNSDVIAIVSEGKIVEKGTHDELIRKSEIYQKFCETQ 1266 HSP 3 Score: 369.007 bits (946), Expect = 5.771e-105 Identity = 217/584 (37.16%), Postives = 336/584 (57.53%), Query Frame = 2
Query: 2357 GCVSAIFNGAVQPSFSILFGFLLPAM--------------ANP------NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLY-----GLTQAAIFCAYAATFMLAGYLLLYNMS-KMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQM 4030
G V+A+ +GA P +I+ G + NP ++++ ++++ Y I + V+ F +S +Q F + E+L + R +A+L+Q I WFD+ G LT RL+D+ ++R G+ ++ + A + F + W ++LV++ F P+I + G + M + ++ +G IA E +IRTV +L + + A+ EV ++ + Y G + ++ +YA F L++ + + +F F A++ G+ +LG ++ +FG + AA+ + +++ P I+ +S +G+++D NM GDI ++V F YP R +I +L +S + + IALVG SGCGKSTI+ LLQRFYD G +LID ++++N+ +LR+ IVSQEP LF +I ENI G+ T DQ++ A K +N +DFI LPD Y T+VGEKG SGGQKQRIAIARAL++NP+ILLLDEATSALDTE+E+ VQ ALD+AQ GRT +++AHRLSTI+N D+I VFK G I ESG+H EL+SK GI+ M
Sbjct: 60 GTVAAVIHGAGFPLLAIVLGGMTTVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLGVLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDK--QQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNAL-----EVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVD--NMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHA--THDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQAQAGRTTIIVAHRLSTIRNVDRIFVFKAGNIVESGSHEELMSKQGIFYDM 632 HSP 4 Score: 332.028 bits (850), Expect = 7.748e-93 Identity = 200/540 (37.04%), Postives = 314/540 (58.15%), Query Frame = 1
Query: 574 NIFTLMSGMSQQFAGIALAMFIMSYVSVTFFV--ITSQHQIS--------AIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG-RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQK 2151
N+++L + Q MF++ + +TFFV TS + + +R F+ ++RQ+I ++D +G L + D V + ++ ++ G + + F GW+L L+ + + PL+++ + + ++ +AG +A + + IRT+ S R E++ Y E L E ++ + G + L+F YA F+ G+ + + D+ VFF+ IG T + +AR AA+ +F +I+ ID +++ G + G I+ + V F YPTR D +L G +L I+ G+TVALVG SGCGKS+++ L++RFY+ ++G ++ID NI++LN+ LREQ+ +VSQEP LF C+I ENI YG +VT +EI +A MAN FI LP+ DT VG++GTQLSGGQKQRIAIARALVR+P +LLLDEATSALD+ESEKIVQ+ALD +GRT +++AHRLST++N+ VI + +GK+ E+GTH EL+ + +Y+ + Q+
Sbjct: 734 NVYSLPADQMQANVYFWCGMFVL--MGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRY-VFTRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQIRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFT-YCEYLREPFNTNLKHAHTYGAVFAFSQSLIFFMYAAAFYLGSIFVNQ-QAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSG-IVKPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNVTYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDAAKQGRTCLVIAHRLSTIQNSDVIAIVSEGKIVEKGTHDELIRKSEIYQKFCETQR 1267
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Celegans
Match: pgp-2 (ABC transporter PGP-2; p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EDF9]) HSP 1 Score: 471.085 bits (1211), Expect = 8.121e-139 Identity = 257/610 (42.13%), Postives = 367/610 (60.16%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIV--RNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDL 2136
+F Y D++++I + ++ G +PL+ +V G T T FL +QN + E +V ++N + I+ L+ G + MF SYV + F ++ + +R + +A++RQ I WFD Q +G LT +L DD+ V G+GDK ++Q +A F+ G + F W +TLV + +PLI++ +SK+M T+ E Y AG IAEE SIRT+ S G K E+ R+ L+ + GI K G +G M+ SYAL FWYG++LI F G + VFF+ L +T +G A PH+ SF AR AA+ + +I+ P+ID + EG +++ +G I+FK V F YP+R D +L G+SL ++ G +ALVG+SGCGKS+++ L+QRFYD +G VLID +++++NV LREQIG+VSQEPVLF +I ENI+ G T +++ +A MANA FI LP+ T VG++G QLSGGQKQRIAIARALV+NPKILLLDEATSALD+E+E+ VQ ALD+ GRTTIIVAHRLST+RN IF K G + E G+H ELM+ G++ D+
Sbjct: 38 IFYYTQGVDLLLLITGTVAAVIHGAGFPLLAIVLGGMT-------------TVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLG---------VLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDKQQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNALEVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVDNMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHATHDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQAQAGRTTIIVAHRLSTIRNVDRIFVFKAGNIVESGSHEELMSKQGIFYDM 625 HSP 2 Score: 421.394 bits (1082), Expect = 3.245e-122 Identity = 237/578 (41.00%), Postives = 358/578 (61.94%), Query Frame = 2
Query: 2309 FKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
FK+ N + + G A G+V P F++++ + + P +Q A++ + + + FV G GE LT + R F+ +L+QDIA++D+ + G L TR + +A +R V L V+ +I ++ A+ I F + WQ++L+++ VP++ +GG ++++ G D LEE+GK+A + +E+IRTV +L R+ F Y E +++ K AH G ++ +Q+ IF YAA F L + + +V+ F A+ F +G + + P+ K ++AA+ +F L++ PI+S S G+V + ++G+I I NV F+YP R + +L + +I + +T+ALVG SGCGKSTI+GLL+RFY+ G I+ID + I+++NI +LR+ IVSQEPTLF +I ENI YG + + T +I+ AAK +NIH+FI+ LPD Y+T VGEKG+ SGGQKQRIAIARAL+R+P +LLLDEATSALDTESEKIVQ+ALD A++GRTC+VIAHRLSTIQN+D I + +GKI E GTH EL+ K+ IY + Q
Sbjct: 712 FKIFKFNGDKVGWFIGGIFGAFIFGSVTPVFALVYAEIFNVYSLPA-DQMQANVYFWCGMFVLMGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRYVF-TRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQIRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFTYCEYLREPFNTNLKHAHTYGAVFAFSQSLIFFMYAAAFYLGSIFVNQQAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSGIV---KPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNV-TYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDAAKQGRTCLVIAHRLSTIQNSDVIAIVSEGKIVEKGTHDELIRKSEIYQKFCETQ 1283 HSP 3 Score: 368.622 bits (945), Expect = 9.731e-105 Identity = 217/584 (37.16%), Postives = 336/584 (57.53%), Query Frame = 2
Query: 2357 GCVSAIFNGAVQPSFSILFGFLLPAM--------------ANP------NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLY-----GLTQAAIFCAYAATFMLAGYLLLYNMS-KMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQM 4030
G V+A+ +GA P +I+ G + NP ++++ ++++ Y I + V+ F +S +Q F + E+L + R +A+L+Q I WFD+ G LT RL+D+ ++R G+ ++ + A + F + W ++LV++ F P+I + G + M + ++ +G IA E +IRTV +L + + A+ EV ++ + Y G + ++ +YA F L++ + + +F F A++ G+ +LG ++ +FG + AA+ + +++ P I+ +S +G+++D NM GDI ++V F YP R +I +L +S + + IALVG SGCGKSTI+ LLQRFYD G +LID ++++N+ +LR+ IVSQEP LF +I ENI G+ T DQ++ A K +N +DFI LPD Y T+VGEKG SGGQKQRIAIARAL++NP+ILLLDEATSALDTE+E+ VQ ALD+AQ GRT +++AHRLSTI+N D+I VFK G I ESG+H EL+SK GI+ M
Sbjct: 53 GTVAAVIHGAGFPLLAIVLGGMTTVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLGVLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDK--QQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNAL-----EVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVD--NMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHA--THDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQAQAGRTTIIVAHRLSTIRNVDRIFVFKAGNIVESGSHEELMSKQGIFYDM 625 HSP 4 Score: 332.413 bits (851), Expect = 8.440e-93 Identity = 200/540 (37.04%), Postives = 314/540 (58.15%), Query Frame = 1
Query: 574 NIFTLMSGMSQQFAGIALAMFIMSYVSVTFFV--ITSQHQIS--------AIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG-RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQK 2151
N+++L + Q MF++ + +TFFV TS + + +R F+ ++RQ+I ++D +G L + D V + ++ ++ G + + F GW+L L+ + + PL+++ + + ++ +AG +A + + IRT+ S R E++ Y E L E ++ + G + L+F YA F+ G+ + + D+ VFF+ IG T + +AR AA+ +F +I+ ID +++ G + G I+ + V F YPTR D +L G +L I+ G+TVALVG SGCGKS+++ L++RFY+ ++G ++ID NI++LN+ LREQ+ +VSQEP LF C+I ENI YG +VT +EI +A MAN FI LP+ DT VG++GTQLSGGQKQRIAIARALVR+P +LLLDEATSALD+ESEKIVQ+ALD +GRT +++AHRLST++N+ VI + +GK+ E+GTH EL+ + +Y+ + Q+
Sbjct: 751 NVYSLPADQMQANVYFWCGMFVL--MGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRY-VFTRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQIRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFT-YCEYLREPFNTNLKHAHTYGAVFAFSQSLIFFMYAAAFYLGSIFVNQ-QAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSG-IVKPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNVTYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDAAKQGRTCLVIAHRLSTIQNSDVIAIVSEGKIVEKGTHDELIRKSEIYQKFCETQR 1284
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Fly
Match: Mdr50 (gene:FBgn0010241 transcript:FBtr0087537) HSP 1 Score: 477.633 bits (1228), Expect = 9.672e-141 Identity = 256/584 (43.84%), Postives = 372/584 (63.70%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA---NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKK-AHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+VM +N PE I +GC+S++ G P F++LFG +L ++ N +++++ + +AGI V +++ LQ FG +GE+LT+R R + FEAML+Q++AWFD+ +N G+L RLS +A ++G +G+ +G ++ +I ++ +A+S + W + LV LAF P I + +Q +M + +E K+AVE + NIRTV++L RE F Q+Y + E+SK+ H +G +YGL ++ +F AYAA + +++ +VF ALI G ++ ++A APN KG AA +F L R P I GV D + G ++ + VKFSYP R I +L + ++ Q IALVGPSGCGKST I L+QRFYD G LID ++++++ NLR IVSQEP LF +IRENI+YGD+A+ TD +II+A K SNIH+FI NLP Y+T++GEKG+ SGGQKQRIAIARAL+RNP+I+LLDEATSALD ESEK+VQDALD A EGRT + IAHRLST+ ++D I VF++G + E+G H +LL+ G+Y + LQS
Sbjct: 732 MLEVMKMNKPEWLQIAVGCISSVIMGCAMPIFAVLFGSILQILSVKDNDQYVRENSNQYSLYFLIAGIVV--GIATFLQIYFFGIAGERLTERLRGLMFEAMLRQEVAWFDDKANGTGSLCARLSGDAAAVQGATGQRIGTIVQSISTLALGIALSMYYEWSLGLVALAFTPFILIAFYMQRTLMAKENMGSAKTMENCTKLAVEVVSNIRTVASLGREEMFHQNYIGMLIPA-VEISKRNTHFRGLVYGLARSLMFFAYAACMYYGTWCVIHRGILFGDVFKVSQALIMGTASIANALAFAPNMQKGVSAAKTIFTFLRRQPSI--VDRPGVSRDPWHSEGYVRFDKVKFSYPTRSEIQVLKGLELAVSKGQKIALVGPSGCGKSTCIQLIQRFYDVDEGATLIDECDVRNVSMTNLRNQLGIVSQEPILFDRTIRENISYGDNARNVTDQEIISACKKSNIHEFIANLPLGYDTRMGEKGAQLSGGQKQRIAIARALIRNPKIMLLDEATSALDAESEKVVQDALDAASEGRTTISIAHRLSTVVHSDVIFVFENGLVCEAGDHKQLLANRGLYYTLYKLQS 1310 HSP 2 Score: 474.167 bits (1219), Expect = 1.325e-139 Identity = 270/619 (43.62%), Postives = 382/619 (61.71%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREG------NGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+LF YAT D + + ++ ++ G+T P L+FGN + M + S + ++ I++ ++ +K F+L ++ I + M + SY+S+T F + QI IR+ FFR+++ Q++ W+D SG + +++ +D++ + G+ +K+ + + F+G ++LAFV+GW+L+LV L+ PL I M +++ +L +K Y A +AE L IRT+ +F GE EVA Y E + A + I++++ +G GL+ ++ SYAL FWYG L+ +G + G M+ VFFS +M + IG A P++ +F A+ A A++F II+++PEI+ ++ EG KLN+ I FK V+F YPTR + IL L+L I GQTVALVG SGCGKS+ IQL+QRFYD G +L + TN+KDL++ WLR +IGVV QEP+LF SI ENIRYGR D T EEI+ A ANA FI LP DTLVG+RG QLSGGQKQRIAIARAL+R+P+ILLLDEATSALD+ SE VQ AL+KV GRTTIIVAHRLSTVR A I I G+V E GTH ELM L Y +LV Q
Sbjct: 75 QLFRYATKKDRALYVIGLLSAVATGLTTPANSLIFGNLANDMIDLGGLLESGKSY--RADDAIST---------LLLDKVRQFSLQNTY--------------IGIIMLVCSYLSITCFNYAAHSQILTIRSKFFRSILHQDMKWYDFNQSGEVASRMNEDLSKMEDGLAEKVVMFVHYLVAFVGSLVLAFVKGWQLSLVCLTSLPLTFIAMGLVAVATSRLAKKEVTMYAGAAVVAEGALSGIRTVKAFEGEAKEVAAYKERVVAAKILNIKRNMFSGIGFGLLWFFIYASYALAFWYGVGLVIKGYHEPAYENYDAGTMITVFFSVMMGSMNIGMAAPYIEAFGIAKGACAKVFHIIEQIPEINPIDGEGKKLNEPLTTIEFKEVEFQYPTRPEVSILNKLNLKIHRGQTVALVGPSGCGKSTCIQLVQRFYDPQAGNLLFNGTNLKDLDINWLRSRIGVVGQEPILFATSIYENIRYGREDATREEIEAAAAAANAAIFIKKLPKGYDTLVGERGAQLSGGQKQRIAIARALIRDPEILLLDEATSALDTASEAKVQAALEKVSAGRTTIIVAHRLSTVRRADRIVVINKGEVVESGTHQELMELKDHYFNLVTTQ 668 HSP 3 Score: 364.385 bits (934), Expect = 5.316e-103 Identity = 236/600 (39.33%), Postives = 334/600 (55.67%), Query Frame = 1
Query: 364 IMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEI-DKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
I S+ MG P+ ++FG S+ Q L S+ D ++ VR ++L + L++G+ GIA +++ + FF I + +R F A++RQ + WFD A +G+L +L D V G +IG ++Q + GI L+ W L LV L+ +P I+I M M K +++ +A EV+ +IRT+ S E+ Y L A +I R + G + GL LMF +YA +YG + G L GD+ V + +M T I A + + SAA IF + R P I D+ D + +G + F VKF+YPTR++ +L GL L++ GQ +ALVG SGCGKS+ IQL+QRFYD +EG LID+ +++++++ LR Q+G+VSQEP+LF +I+ENI YG +VTD+EI A +N FI LP DT +G++G QLSGGQKQRIAIARAL+RNPKI+LLDEATSALD+ESEK+VQDALD EGRTTI +AHRLSTV ++ VIF ++G V E G H +L+A GLY L Q
Sbjct: 751 ISSVIMGCAMPIFAVLFG--------------SILQIL-----------SVKDNDQYVRENSNQYSL-----YFLIAGI---VVGIA------TFLQIYFFGIAGERLTERLRGLMFEAMLRQEVAWFDDKANGTGSLCARLSGDAAAVQGATGQRIGTIVQSISTLALGIALSMYYEWSLGLVALAFTPFILIAFYMQRTLMAKENMGSAKTMENCTKLAVEVVSNIRTVASLGREEMFHQNYIGMLIPAVEISKRNTHFRGLVYGLARSLMFFAYAACMYYGTWCVIH-RGILFGDVFKVSQALIMGTASIANALAFAPNMQKGVSAAKTIFTFLRRQPSIVDRPGVSRDPWHS-EGYVRFDKVKFSYPTRSEIQVLKGLELAVSKGQKIALVGPSGCGKSTCIQLIQRFYDVDEGATLIDECDVRNVSMTNLRNQLGIVSQEPILFDRTIRENISYGDNARNVTDQEIISACKKSNIHEFIANLPLGYDTRMGEKGAQLSGGQKQRIAIARALIRNPKIMLLDEATSALDAESEKVVQDALDAASEGRTTISIAHRLSTVVHSDVIFVFENGLVCEAGDHKQLLANRGLYYTLYKLQ 1309 HSP 4 Score: 300.827 bits (769), Expect = 1.683e-82 Identity = 209/594 (35.19%), Postives = 316/594 (53.20%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAM--------------ANPNIEQQSAD-IRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL----------YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
++ +G +SA+ G P+ S++FG L M A+ I D +R + + I ++ V S L F + RS F ++L QD+ W+D N G + +R++++ +++ E + + + + ++ ++F WQ+SLV L +P+ F+ L + + + +A + IRTV E + YKE + K+ G +GL I+ +YA F L++ Y+ M VF S ++ G+MN+G + FG K A A +F +++++P IN +G L+ E ++ I+ + V+F YP RP + ILN ++ I+ QT+ALVGPSGCGKST I L+QRFYD GN+L + +KD++I LR +V QEP LF+ SI ENI YG T ++I AAA A+N FI LP Y+T VGE+G+ SGGQKQRIAIARAL+R+P ILLLDEATSALDT SE VQ AL+K GRT +++AHRLST++ AD+I+V G++ ESGTH EL+ Y +++ Q
Sbjct: 84 DRALYVIGLLSAVATGLTTPANSLIFGNLANDMIDLGGLLESGKSYRADDAISTLLLDKVRQFSLQNTYIGIIMLVCSYLSITCFNYAAHSQILTIRSKFFRSILHQDMKWYD--FNQSGEVASRMNEDLSKMEDGLAEKVVMFVHYLVAFVGSLVLAFVKGWQLSLVCLTSLPLTFIAMGLVAVATSRLAKKEVTMYAGAAVVAEGALSGIRTVKAFEGEAKEVAAYKERVVAAKILNIKRNMFSGIGFGLLWFFIYASYALAFWYGVGLVIKGYHEPAYENYDAGTMITVFFS---VMMGSMNIGMAAPYIEAFGIAKGACAKVFHIIEQIPEINPIDGEGKKLN-EPLT-TIEFKEVEFQYPTRPEVSILNKLNLKIHRGQTVALVGPSGCGKSTCIQLVQRFYDPQAGNLLFNGTNLKDLDINWLRSRIGVVGQEPILFATSIYENIRYGREDA--TREEIEAAAAAANAAIFIKKLPKGYDTLVGERGAQLSGGQKQRIAIARALIRDPEILLLDEATSALDTASEAKVQAALEKVSAGRTTIIVAHRLSTVRRADRIVVINKGEVVESGTHQELMELKDHYFNLVTTQ 668
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Fly
Match: Mdr65 (gene:FBgn0004513 transcript:FBtr0077011) HSP 1 Score: 449.129 bits (1154), Expect = 3.579e-131 Identity = 242/584 (41.44%), Postives = 363/584 (62.16%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALA--GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINS--FSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXX-XXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F +++ L E CY+ LG +SA+ G + P+F+++FG A+A + E A RT V++ A G+A + + LQ F +G LT R R++TF AM+ Q++ WFD+ +N++GAL+ RLS EA I+G G L +I A+ + ++V+++ + W+++L+ LA PII IL+ ++M +K +EE+ +IA E I NIRTV+ L RE I+ Y E I++++ + +K +G L QA+ F AYA G L+ ++ L++G+M L +S+A P F IA +F++LDR P I S + + N+ ++ ++F YP RP+ ILN + + QT+ALVG SGCGKST + LLQR+YD + ++ D+ + +R IVSQEPTLF SI ENIAYGD+ + + +IIAAAK++N H FI++LP+ Y+T++G +G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD +SE++VQ ALD A GRTC+VIAHRLST+QNAD I V ++G++ E G H +L+S+ GIY+++ Q
Sbjct: 718 FSRILQLAKQEWCYLILGTISAVAVGFLYPAFAVIFGEFYAALAEKDPE--DALRRTAVLSWACLGLAFLTGLVCFLQTYLFNYAGIWLTTRMRAMTFNAMVNQEVGWFDDENNSVGALSARLSGEAVDIQGAIGYPLSGMIQALSNFISSVSVAMYYNWKLALLCLANCPIIVGSVILEAKMMSNAVVREKQVIEEACRIATESITNIRTVAGLRREADVIREYTEEIQRVEVLIRQKLRWRGVLNSTMQASAFFAYAVALCYGGVLVSEGQLPFQDIIKVSETLLYGSMMLAQSLAFTPAFSAALIAGHRLFQILDRKPKIQSPMGTIKNTLAKQLNLFEGVRYRGIQFRYPTRPDAKILNGLDLEVLKGQTVALVGHSGCGKSTCVQLLQRYYDPDEGTIHIDHDDIQHDLTLDGVRTKLGIVSQEPTLFERSIAENIAYGDNRRSVSMVEIIAAAKSANAHSFIISLPNGYDTRMGARGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDLQSEQLVQQALDTACSGRTCIVIAHRLSTVQNADVICVIQNGQVVEQGNHMQLISQGGIYAKLHKTQ 1299 HSP 2 Score: 432.565 bits (1111), Expect = 1.127e-125 Identity = 252/626 (40.26%), Postives = 365/626 (58.31%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQN----------EPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLI-----REGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLN-DFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
KLF ++T +I + IM +T P +++++ TS + ++ QF TS N + +T+++ + E + Y+ ++S + T+ S + MFI SV F + + Q++ +R F +V+RQ+IGW D + T +VDD+ + GI +K+G + FI + ++F GWKLTL S PL+I+ ++K KLT + E+Y AG +AEE+L SIRT+VSF GEK EV RY L A K K +G ++ +++ S A FWYG +LI E + ++I FF ++ I + P + SF AR A +F++ID +ID ++ +G LN +G + F+ V F YP+R + + GL++ I+ GQTVALVG+SGCGKS+ +QL+QRFYD G VL+DD +I+ N+ WLR I VV QEPVLF +I +NI YG+ T +EI+ A A A FI LP +++G+RG+QLSGGQKQRIAIARAL++NPKILLLDEATSALD +SEK VQ ALD +GRTTI+V+HRLS +R A I I DGKV E+G+H +LMAL G Y ++V
Sbjct: 35 KLFRFSTYGEIGWLFFGFIMCCIKALTLPAVVIIYSEFTSML------VDRAMQFGTSSNVHALPLFGGGKTLTNASREENNEAL-----YDDSISYGILLTIAS----------VVMFISGIFSVDVFNMVALRQVTRMRIKLFSSVIRQDIGWHDLASKQNFTQSMVDDVEKIRDGISEKVGHFVYLVVGFIITVAISFSYGWKLTLAVSSYIPLVILLNYYVAKFQGKLTAREQESYAGAGNLAEEILSSIRTVVSFGGEKSEVQRYENFLVPARKASQWKGAFSGLSDAVLKSMLYLSCAGAFWYGVNLIIDDRNVENKEYTPAILMIAFFGIIVGADNIARTAPFLESFATARGCATNLFKVIDLTSKIDPLSTDGKLLNYGLRGDVEFQDVFFRYPSRPEVIVHRGLNIRIRAGQTVALVGSSGCGKSTCVQLLQRFYDPVFGSVLLDDLDIRKYNIQWLRSNIAVVGQEPVLFLGTIAQNISYGKPGATQKEIEAAATQAGAHEFITNLPESYRSMIGERGSQLSGGQKQRIAIARALIQNPKILLLDEATSALDYQSEKQVQQALDLASKGRTTIVVSHRLSAIRGADKIVFIHDGKVLEEGSHDDLMALEGAYYNMV 639 HSP 3 Score: 352.058 bits (902), Expect = 4.425e-99 Identity = 215/525 (40.95%), Postives = 312/525 (59.43%), Query Frame = 1
Query: 616 GIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIX-XXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDK-----MNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIK-DLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNR 2157
G+A ++ ++ F + +R F A++ Q +GWFD +N+ GAL+ +L + + IG + M+Q + FI + +A WKL L+ L+ P+I+ +++ +K M + + +A IA E + +IRT+ R E + YTE + + ++ IR+ + G L + F +YA+ YG L+ EG D++ V + L + + Q+ +F+ A A +F+I+DR P+I N +LN F+G + ++ ++F YPTR D IL GL L + GQTVALVG SGCGKS+ +QL+QR+YD +EG + ID +I+ DL + +R ++G+VSQEP LF SI ENI YG R V+ EI A ANA +FI +LPN DT +G RGTQLSGGQKQRIAIARALVRNPKILLLDEATSALD +SE++VQ ALD GRT I++AHRLSTV+NA VI I++G+V EQG H +L++ G+Y L QK+
Sbjct: 781 GLAFLTGLVCFLQTYLFNYAGIWLTTRMRAMTFNAMVNQEVGWFDDENNSVGALSARLSGEAVDIQGAIGYPLSGMIQALSNFISSVSVAMYYNWKLALLCLANCPIIVGSVILEAKMMSNAVVREKQVIEEACRIATESITNIRTVAGLRREADVIREYTEEI-QRVEVLIRQKLRWRGVLNSTMQASAFFAYAVALCYGGVLVSEGQ-LPFQDIIKVSETLLYGSMMLAQSLAFTPAFSAALIAGHRLFQILDRKPKIQSPMGTIKNTLAKQLNLFEG-VRYRGIQFRYPTRPDAKILNGLDLEVLKGQTVALVGHSGCGKSTCVQLLQRYYDPDEGTIHIDHDDIQHDLTLDGVRTKLGIVSQEPTLFERSIAENIAYGDNRRSVSMVEIIAAAKSANAHSFIISLPNGYDTRMGARGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDLQSEQLVQQALDTACSGRTCIVIAHRLSTVQNADVICVIQNGQVVEQGNHMQLISQGGIYAKLHKTQKDH 1302 HSP 4 Score: 322.783 bits (826), Expect = 1.539e-89 Identity = 199/552 (36.05%), Postives = 309/552 (55.98%), Query Frame = 2
Query: 2408 LFG---FLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTN-------VFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMI 4033
LFG L A N E D +Y I L +VV F+S + F + R R F ++++QDI W D S T + D+ +IR E +G + + VAISF + W+++L V +++P++ + + + ++ + +G +A E + +IRTV + E + +Q Y+ + K G + ++ ++ + A F G L+ + + N + I+F +I GA N+ ++ +F + A ++F+++D I+ STDG +L+ + GD++ ++V F YP RP +I+ ++ I + QT+ALVG SGCGKST + LLQRFYD + G++L+D+ I+ NIQ LR ++V QEP LF +I +NI+YG T +I AAA + H+FI NLP+ Y + +GE+GS SGGQKQRIAIARAL++NP+ILLLDEATSALD +SEK VQ ALD A +GRT +V++HRLS I+ ADKI+ DGK+ E G+H +L++ G Y M+
Sbjct: 94 LFGGGKTLTNASREENNEALYDDSISYGILLTIASVVMFISGIFSVDVFNMVALRQVTRMRIKLFSSVIRQDIGWHDLASKQ--NFTQSMVDDVEKIRDGISEKVGHFVYLVVGFIITVAISFSYGWKLTLAVSSYIPLVILLNYYVAKFQGKLTAREQESYAGAGNLAEEILSSIRTVVSFGGEKSEVQRYENFLVPARKASQWKGAFSGLSDAVLKSMLYLSCAGAFWY-GVNLIIDDRNVENKEYTPAILMIAFFGIIVGADNIARTAPFLESFATARGCATNLFKVIDLTSKIDPLSTDGKLLNY-GLRGDVEFQDVFFRYPSRPEVIVHRGLNIRIRAGQTVALVGSSGCGKSTCVQLLQRFYDPVFGSVLLDDLDIRKYNIQWLRSNIAVVGQEPVLFLGTIAQNISYGKPGA--TQKEIEAAATQAGAHEFITNLPESYRSMIGERGSQLSGGQKQRIAIARALIQNPKILLLDEATSALDYQSEKQVQQALDLASKGRTTIVVSHRLSAIRGADKIVFIHDGKVLEEGSHDDLMALEGAYYNMV 639
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Fly
Match: Mdr49 (gene:FBgn0004512 transcript:FBtr0087801) HSP 1 Score: 436.032 bits (1120), Expect = 7.284e-127 Identity = 247/633 (39.02%), Postives = 371/633 (58.61%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNS------LTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNG-----FLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRRE 2178
LF Y+T + +++ +++++ P ++++G TS + + + L F Q +T+++ + + I+ + ++ I +L+ ++AMF++ +++ + +QI IR F A++RQ+I W+D + +K+ +D++ + GIG+KI ++ F+ GI+ AFV GWKLTLV LS P II ++++ L +K ++Y A + EEV IRT+ +F G++ E R+ + L A G +K + +G L +++ AL WYG +LI + + ++IV F+ +M +G A+PH+ + A +A +F IIDR ++D M+E+G++ + G I F+ ++F YP R D IL GL++ + PGQTVA VGASGCGKS+LIQLMQRFYD G V +D +++ LNV WLR QIGVV QEPVLF +I ENIRYGR T +I++A AN FI LP DT VG++G Q+SGGQKQRIAIARALVR P++LLLDEATSALD SEK VQ AL+ +G TT++VAHRLST+ NA I +KDG V EQGTH ELM GLY +LV + + T+ E
Sbjct: 34 LFRYSTRCERFLLVVSLLVATAASAFIPYFMIIYGEFTSLLVDRTVGVGTSSPAFALPMFGGGQQ--LTNASKEENNQAIIDDA------TAFGIGSLVG---------SVAMFLLITLAIDLANRIALNQIDRIRKLFLEAMLRQDIAWYDTSSGSNFASKMTEDLDKLKEGIGEKIVIVVFLIMTFVIGIVSAFVYGWKLTLVVLSCVPFIIAATSVVARLQGSLAEKELKSYSDAANVVEEVFSGIRTVFAFSGQEKEKERFGKLLIPAENTGRKKGLYSGMGNALSWLIIYLCMALAIWYGVTLILDERDLPDRVYTPAVLVIVLFAVIMGAQNLGFASPHVEAIAVATAAGQTLFNIIDRPSQVDPMDEKGNRPENTAGHIRFEGIRFRYPARPDVEILKGLTVDVLPGQTVAFVGASGCGKSTLIQLMQRFYDPEAGSVKLDGRDLRTLNVGWLRSQIGVVGQEPVLFATTIGENIRYGRPSATQADIEKAARAANCHDFITRLPKGYDTQVGEKGAQISGGQKQRIAIARALVRQPQVLLLDEATSALDPTSEKRVQSALELASQGPTTLVVAHRLSTITNADKIVFLKDGVVAEQGTHEELMERRGLYCELVSITQRKEATEADE 649 HSP 2 Score: 407.912 bits (1047), Expect = 1.676e-117 Identity = 231/592 (39.02%), Postives = 356/592 (60.14%), Query Frame = 2
Query: 2294 SSAGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRV-----PPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDD----QIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
S F ++M LN+PE +I +G ++++ +GA P + + FG +++ + + A++ + GI ++A + ++LQ F +G ++T R R F ++ QDIA+FD+ N++GAL +RL+ + + ++G +G +G ++ A+ ++ + + F F WQ +L+ L +P++ + L+ R + + K ++EE+ ++AVE I NIRTV+ L E + Y + I ++D +K +G ++ L QAA F AY + G L+ ++ ALIFG+ LG+++A APN ++A + +L R PP + ++T VE GDI ENV F YP R IL ++ I + T+ALVGPSG GKST + LL R+YD ++G++ + + + LR +VSQEP LF +I ENIAYG++ F DD +II AAK SNIH+FI LP Y+T++G K S SGGQKQRIAIARAL+RNP+IL+LDEATSALD ESEK+VQ ALD+A+ GRTC+ IAHRL+T++NAD I V K G + E GTH EL++ N IY+ + +Q
Sbjct: 717 SKVSFTQLMKLNSPEWRFIVVGGIASVMHGATFPLWGLFFGDFFGILSDGDDDVVRAEVLKISMIFVGIGLMAGLGNMLQTYMFTTAGVKMTTRLRKRAFGTIIGQDIAYFDDERNSVGALCSRLASDCSNVQGATGARVGTMLQAVATLVVGMVVGFVFSWQQTLLTLVTLPLVCLSVYLEGRFIMKSAQKAKASIEEASQVAVEAITNIRTVNGLCLERQVLDQYVQQIDRVDIACRRKVRFRGLVFALGQAAPFLAYGISMYYGGILVAEERMNYEDIIKVAEALIFGSWMLGQALAYAPNVNDAILSAGRLMDLFKRTSTQPNPPQSPYNT------VEKSEGDIVYENVGFEYPTRKGTPILQGLNLTIKKSTTVALVGPSGSGKSTCVQLLLRYYDPVSGSVNLSGVPSTEFPLDTLRSKLGLVSQEPVLFDRTIAENIAYGNN---FRDDVSMQEIIEAAKKSNIHNFISALPQGYDTRLG-KTSQLSGGQKQRIAIARALVRNPKILILDEATSALDLESEKVVQQALDEARSGRTCLTIAHRLTTVRNADLICVLKRGVVVEHGTHDELMALNKIYANLYLMQ 1298 HSP 3 Score: 318.161 bits (814), Expect = 4.870e-88 Identity = 185/499 (37.07%), Postives = 279/499 (55.91%), Query Frame = 2
Query: 2573 RCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVF------ISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 4051
R R + EAML+QDIAW+D S + ++++++ +++ GE + V+ I + + +F + W+++LVVL+ VP I + R+ + + + ++ + E IRTV + + + + + + + KK G L+ I+ A L+L V+ I A+I GA NLG + A +F ++DR ++ G EN +G I+ E ++F YP RP++ IL ++ ++ QT+A VG SGCGKST+I L+QRFYD G++ +D ++ +N+ LR +V QEP LF+ +I ENI YG + D I AA+A+N HDFI LP Y+T+VGEKG+ SGGQKQRIAIARAL+R P++LLLDEATSALD SEK VQ AL+ A +G T +V+AHRLSTI NADKI+ KDG +AE GTH EL+ + G+Y +++S+ K
Sbjct: 150 RIRKLFLEAMLRQDIAWYDTSSGS--NFASKMTEDLDKLKEGIGEKIVIVVFLIMTFVIGIVSAFVYGWKLTLVVLSCVPFIIAATSVVARLQGSLAEKELKSYSDAANVVEEVFSGIRTVFAFSGQEKEKERFGKLLIPAENTGRKKGLYSGMGNALSWLIIYLCMALAIWYGVTLILDERDLPDRVYTPAVLVIVLFAVIMGAQNLGFASPHVEAIAVATAAGQTLFNIIDRPSQVDPMDEKGN--RPENTAGHIRFEGIRFRYPARPDVEILKGLTVDVLPGQTVAFVGASGCGKSTLIQLMQRFYDPEAGSVKLDGRDLRTLNVGWLRSQIGVVGQEPVLFATTIGENIRYGRPSATQAD--IEKAARAANCHDFITRLPKGYDTQVGEKGAQISGGQKQRIAIARALVRQPQVLLLDEATSALDPTSEKRVQSALELASQGPTTLVVAHRLSTITNADKIVFLKDGVVAEQGTHEELMERRGLYCELVSITQRK 642 HSP 4 Score: 295.819 bits (756), Expect = 6.659e-81 Identity = 202/522 (38.70%), Postives = 301/522 (57.66%), Query Frame = 1
Query: 598 MSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDE---ACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVP-EIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG---RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDL 2136
+S F GI L + + + F + +R F ++ Q+I +FD + + GAL ++L D + V G ++G M+Q A + G+++ FV W+ TL+TL PL+ + + + + +MK QKA + +A +A E + +IRT+ E+ + +Y + +D AC+ RK G + L F +Y + +YG L+ E D++ V + + + +GQA + + N+A +A + ++ R + + + + +G I ++ V F YPTR PIL GL+L+I+ TVALVG SG GKS+ +QL+ R+YD G V + + + LR ++G+VSQEPVLF +I ENI YG R DV+ +EI +A +N FI+ALP DT +G + +QLSGGQKQRIAIARALVRNPKIL+LDEATSALD ESEK+VQ ALD+ GRT + +AHRL+TVRNA +I +K G V E GTH ELMALN +Y +L
Sbjct: 778 ISMIFVGIGLMAGLGNMLQTYMFTTAGVKMTTRLRKRAFGTIIGQDIAYFDDERNSVGALCSRLASDCSNVQGATGARVGTMLQAVATLVVGMVVGFVFSWQQTLLTLVTLPLVCLSVYLEGRFIMKSAQKAKASIEEASQVAVEAITNIRTVNGLCLERQVLDQYVQQIDRVDIACR---RKVRFRGLVFALGQAAPFLAYGISMYYGGILVAEER-MNYEDIIKVAEALIFGSWMLGQALAYAPNVNDAILSAGRLMDLFKRTSTQPNPPQSPYNTVEKSEGDIVYENVGFEYPTRKGTPILQGLNLTIKKSTTVALVGPSGSGKSTCVQLLLRYYDPVSGSVNLSGVPSTEFPLDTLRSKLGLVSQEPVLFDRTIAENIAYGNNFRDDVSMQEIIEAAKKSNIHNFISALPQGYDTRLG-KTSQLSGGQKQRIAIARALVRNPKILILDEATSALDLESEKVVQQALDEARSGRTCLTIAHRLTTVRNADLICVLKRGVVVEHGTHDELMALNKIYANL 1294
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Fly
Match: CG10226 (gene:FBgn0035695 transcript:FBtr0332068) HSP 1 Score: 429.483 bits (1103), Expect = 2.331e-125 Identity = 231/591 (39.09%), Postives = 352/591 (59.56%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQ---QSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFST-------DGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXX-XXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
FF+++G PE ++ +G + A G P FS++ L ++A P E+ QSA + +I+L I + A V +Q F +G LT R RS TF ++ Q++ WFD N+IGAL+ RLS +A ++G G L +I A + ++AI+F + W+++L+ L+ P + + + R + + +K LEE+ +IA E I IRTV+ L RE I+ Y + +++ ++ + +G + L ++ +F YA T G++ K + +++G L +S+A P F ++A M+E++DR P I S + +G + + + FSYP RP+I +L + + +IN QT+ALVG SG GKST + LL R+YD ++ + D++++ LR+ IVSQEP+LF SI +NI YGD+++ QII AAK +N H+FI++LP QY+T +G KG+ SGGQKQRIAIARA++RNP+ILLLDEATSALD +SE++VQ ALD A GRTC+VIAHRLSTIQNA+ I V + GKI E G+H +LL+KNGIYS++ Q+
Sbjct: 616 FFRILGWARPEWSFLIIGAICAGLYGVTMPVFSVVLAELYGSLAKPTDEEVLEQSASMA--IISLV-IGIAAGVVCYIQTFFFNLAGVWLTTRMRSKTFRCIMNQEMGWFDRKENSIGALSARLSGDAASVQGAIGFPLSNIIQAFTNFICSIAIAFPYSWELALICLSTSPFMVASIVFEARFGEKSALKEKEVLEETSRIATETITQIRTVAGLRREEELIKIYDKEVERYRHQILSRLKWRGLVNSLGKSLMFFGYAVTLTYGGHMCADGKIKFETIMKISNTMLYGLFILAQSLAFTPAFNAALLSANRMYEIIDRKPQIQSPESFEIQQNGNGTAYKTNVVQQGVSYRGLNFSYPSRPHIKVLQNFNLDINQGQTVALVGASGSGKSTCVQLLMRYYDPDEGKILIDQESIHHDMDLKTLRRRLGIVSQEPSLFEKSIADNIGYGDTSRQVPMQQIIEAAKMANAHEFIMSLPAQYDTVLGSKGTQLSGGQKQRIAIARAMVRNPKILLLDEATSALDFQSERVVQQALDSACSGRTCIVIAHRLSTIQNANVICVIQAGKIVEQGSHSQLLAKNGIYSKLYRCQT 1203 HSP 2 Score: 396.356 bits (1017), Expect = 2.631e-114 Identity = 211/491 (42.97%), Postives = 305/491 (62.12%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIR---------EGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLN-DFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
+R FF+A +RQ IGW D ++ D++ + +GI + +G ++ I ++L+F+ GWKL L + PL ++ ++ KLT + +Y +A + EEV+G+IRT+V+F GE+ E RY L A K G K +G ++ ++F + A FWYGA+LI + + ++IV +++ I + +P + +F AR +A+ I ++IDR ID +++ G LN +G + F+ V F YP R D +L GL++ ++ GQTVALVG SGCGKS+ IQL+QRFYD G+VL+D +++ N+ WLR I VV QEPVLF SI ENIR+G+ + T +E++ A ANA FI AL DT + ++G QLSGGQ+QRIAIARAL++ PKILLLDEATSALD SEK+VQ ALDK +GRTT++V+HRLS +R+AH I I++GK EQGTH ELM L G Y +V
Sbjct: 44 MRREFFKATLRQEIGWHDMAKDQNFAVRITDNMEKIRSGIAENLGHYVEIMCDVIISVVLSFIYGWKLALAIVFYIPLTLVVNSAVAHYQGKLTGQEQSSYVRASSVVEEVIGAIRTVVAFGGERSESLRYDSLLKPALKAGKWKGAFSGLSDTVMKAMLFITGAGSFWYGANLILYYRDPSIPIDEREYTPAVVMIVISGIIVSANQISRTSPFLETFAMARGSASAILDVIDRTSLIDPLSKAGKILNYGLKGAVEFRDVFFRYPAREDVIVLRGLNVVVEEGQTVALVGPSGCGKSTCIQLLQRFYDPVFGQVLLDGEDVRKYNIKWLRSNIAVVGQEPVLFQGSIGENIRHGKPEATQKEVEDAAKAANAHDFIIALHKGYDTDISEKGVQLSGGQRQRIAIARALIQQPKILLLDEATSALDYHSEKLVQAALDKACKGRTTLVVSHRLSAIRHAHRIVYIENGKAVEQGTHEELMKLEGFYHKMV 534 HSP 3 Score: 347.051 bits (889), Expect = 5.620e-98 Identity = 208/528 (39.39%), Postives = 309/528 (58.52%), Query Frame = 1
Query: 619 IALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEK-------YEVARYTENLDEACKI-GIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEID-----KMNEEGD----KLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIK-DLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDL 2136
I +A ++ Y+ FF + + +R+ FR +M Q +GWFD +NS GAL+ +L D +V IG + ++Q + FI I +AF W+L L+ LS SP ++ ++ ++ K K E + IA E + IRT+ R E+ EV RY + K G+ S+ + YA+ YG + +G ++ + + L + Q+ +FN A +A ++EIIDR P+I ++ + G+ K N Q ++++ + F+YP+R +L +L I GQTVALVGASG GKS+ +QL+ R+YD +EG++LID +I D+++ LR ++G+VSQEP LF SI +NI YG V ++I +A MANA FI +LP + DT++G +GTQLSGGQKQRIAIARA+VRNPKILLLDEATSALD +SE++VQ ALD GRT I++AHRLST++NA+VI I+ GK+ EQG+H++L+A NG+Y L
Sbjct: 680 IGIAAGVVCYIQTFFFNLAGVWLTTRMRSKTFRCIMNQEMGWFDRKENSIGALSARLSGDAASVQGAIGFPLSNIIQAFTNFICSIAIAFPYSWELALICLSTSPFMVASIVFEARFGEKSALKEKEVLEETSRIATETITQIRTVAGLRREEELIKIYDKEVERYRHQILSRLKWRGLVNSLGKSLMFF--------GYAVTLTYGGHMCADGK-IKFETIMKISNTMLYGLFILAQSLAFTPAFNAALLSANRMYEIIDRKPQIQSPESFEIQQNGNGTAYKTNVVQQGVSYRGLNFSYPSRPHIKVLQNFNLDINQGQTVALVGASGSGKSTCVQLLMRYYDPDEGKILIDQESIHHDMDLKTLRRRLGIVSQEPSLFEKSIADNIGYGDTSRQVPMQQIIEAAKMANAHEFIMSLPAQYDTVLGSKGTQLSGGQKQRIAIARAMVRNPKILLLDEATSALDFQSERVVQQALDSACSGRTCIVIAHRLSTIQNANVICVIQAGKIVEQGSHSQLLAKNGIYSKL 1198 HSP 4 Score: 300.056 bits (767), Expect = 1.238e-82 Identity = 181/506 (35.77%), Postives = 280/506 (55.34%), Query Frame = 2
Query: 2561 QLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLY--------NMSKMTN--VFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
+LT R R F+A L+Q+I W D + R++D +IR E+LG + +C + +V +SF + W+++L ++ ++P+ V + ++ + + + E I IRTV E + Y +K K G + +A +F A +F L+LY + + T V I +I A + ++ F + +A+ + +++DR I+ S G +L+ + G ++ +V F YP R ++I+L ++ + QT+ALVGPSGCGKST I LLQRFYD + G +L+D ++ NI+ LR ++V QEP LF SI ENI +G T ++ AAKA+N HDFI+ L Y+T + EKG SGGQ+QRIAIARAL++ P+ILLLDEATSALD SEK+VQ ALDKA +GRT +V++HRLS I++A +I+ ++GK E GTH EL+ G Y +M+++ S+
Sbjct: 39 KLTVRMRREFFKATLRQEIGWHDMAKDQ--NFAVRITDNMEKIRSGIAENLGHYVEIMCDVIISVVLSFIYGWKLALAIVFYIPLTLVVNSAVAHYQGKLTGQEQSSYVRASSVVEEVIGAIRTVVAFGGERSESLRYDSLLKPALKAGKWKGAFSGLSDTVMKAMLFITGAGSFWYGANLILYYRDPSIPIDEREYTPAVVMIVISGIIVSANQISRTSPFLETFAMARGSASAILDVIDRTSLIDPLSKAGKILNY-GLKGAVEFRDVFFRYPAREDVIVLRGLNVVVEEGQTVALVGPSGCGKSTCIQLLQRFYDPVFGQVLLDGEDVRKYNIKWLRSNIAVVGQEPVLFQGSIGENIRHGKPEA--TQKEVEDAAKAANAHDFIIALHKGYDTDISEKGVQLSGGQRQRIAIARALIQQPKILLLDEATSALDYHSEKLVQAALDKACKGRTTLVVSHRLSAIRHAHRIVYIENGKAVEQGTHEELMKLEGFYHKMVTVHSY 539
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Fly
Match: CG10226 (gene:FBgn0035695 transcript:FBtr0077012) HSP 1 Score: 429.483 bits (1103), Expect = 1.702e-124 Identity = 231/591 (39.09%), Postives = 352/591 (59.56%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQ---QSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFST-------DGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXX-XXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
FF+++G PE ++ +G + A G P FS++ L ++A P E+ QSA + +I+L I + A V +Q F +G LT R RS TF ++ Q++ WFD N+IGAL+ RLS +A ++G G L +I A + ++AI+F + W+++L+ L+ P + + + R + + +K LEE+ +IA E I IRTV+ L RE I+ Y + +++ ++ + +G + L ++ +F YA T G++ K + +++G L +S+A P F ++A M+E++DR P I S + +G + + + FSYP RP+I +L + + +IN QT+ALVG SG GKST + LL R+YD ++ + D++++ LR+ IVSQEP+LF SI +NI YGD+++ QII AAK +N H+FI++LP QY+T +G KG+ SGGQKQRIAIARA++RNP+ILLLDEATSALD +SE++VQ ALD A GRTC+VIAHRLSTIQNA+ I V + GKI E G+H +LL+KNGIYS++ Q+
Sbjct: 730 FFRILGWARPEWSFLIIGAICAGLYGVTMPVFSVVLAELYGSLAKPTDEEVLEQSASMA--IISLV-IGIAAGVVCYIQTFFFNLAGVWLTTRMRSKTFRCIMNQEMGWFDRKENSIGALSARLSGDAASVQGAIGFPLSNIIQAFTNFICSIAIAFPYSWELALICLSTSPFMVASIVFEARFGEKSALKEKEVLEETSRIATETITQIRTVAGLRREEELIKIYDKEVERYRHQILSRLKWRGLVNSLGKSLMFFGYAVTLTYGGHMCADGKIKFETIMKISNTMLYGLFILAQSLAFTPAFNAALLSANRMYEIIDRKPQIQSPESFEIQQNGNGTAYKTNVVQQGVSYRGLNFSYPSRPHIKVLQNFNLDINQGQTVALVGASGSGKSTCVQLLMRYYDPDEGKILIDQESIHHDMDLKTLRRRLGIVSQEPSLFEKSIADNIGYGDTSRQVPMQQIIEAAKMANAHEFIMSLPAQYDTVLGSKGTQLSGGQKQRIAIARAMVRNPKILLLDEATSALDFQSERVVQQALDSACSGRTCIVIAHRLSTIQNANVICVIQAGKIVEQGSHSQLLAKNGIYSKLYRCQT 1317 HSP 2 Score: 396.356 bits (1017), Expect = 1.476e-113 Identity = 211/491 (42.97%), Postives = 305/491 (62.12%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIR---------EGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLN-DFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
+R FF+A +RQ IGW D ++ D++ + +GI + +G ++ I ++L+F+ GWKL L + PL ++ ++ KLT + +Y +A + EEV+G+IRT+V+F GE+ E RY L A K G K +G ++ ++F + A FWYGA+LI + + ++IV +++ I + +P + +F AR +A+ I ++IDR ID +++ G LN +G + F+ V F YP R D +L GL++ ++ GQTVALVG SGCGKS+ IQL+QRFYD G+VL+D +++ N+ WLR I VV QEPVLF SI ENIR+G+ + T +E++ A ANA FI AL DT + ++G QLSGGQ+QRIAIARAL++ PKILLLDEATSALD SEK+VQ ALDK +GRTT++V+HRLS +R+AH I I++GK EQGTH ELM L G Y +V
Sbjct: 158 MRREFFKATLRQEIGWHDMAKDQNFAVRITDNMEKIRSGIAENLGHYVEIMCDVIISVVLSFIYGWKLALAIVFYIPLTLVVNSAVAHYQGKLTGQEQSSYVRASSVVEEVIGAIRTVVAFGGERSESLRYDSLLKPALKAGKWKGAFSGLSDTVMKAMLFITGAGSFWYGANLILYYRDPSIPIDEREYTPAVVMIVISGIIVSANQISRTSPFLETFAMARGSASAILDVIDRTSLIDPLSKAGKILNYGLKGAVEFRDVFFRYPAREDVIVLRGLNVVVEEGQTVALVGPSGCGKSTCIQLLQRFYDPVFGQVLLDGEDVRKYNIKWLRSNIAVVGQEPVLFQGSIGENIRHGKPEATQKEVEDAAKAANAHDFIIALHKGYDTDISEKGVQLSGGQRQRIAIARALIQQPKILLLDEATSALDYHSEKLVQAALDKACKGRTTLVVSHRLSAIRHAHRIVYIENGKAVEQGTHEELMKLEGFYHKMV 648 HSP 3 Score: 347.051 bits (889), Expect = 2.426e-97 Identity = 208/528 (39.39%), Postives = 309/528 (58.52%), Query Frame = 1
Query: 619 IALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEK-------YEVARYTENLDEACKI-GIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEID-----KMNEEGD----KLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIK-DLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDL 2136
I +A ++ Y+ FF + + +R+ FR +M Q +GWFD +NS GAL+ +L D +V IG + ++Q + FI I +AF W+L L+ LS SP ++ ++ ++ K K E + IA E + IRT+ R E+ EV RY + K G+ S+ + YA+ YG + +G ++ + + L + Q+ +FN A +A ++EIIDR P+I ++ + G+ K N Q ++++ + F+YP+R +L +L I GQTVALVGASG GKS+ +QL+ R+YD +EG++LID +I D+++ LR ++G+VSQEP LF SI +NI YG V ++I +A MANA FI +LP + DT++G +GTQLSGGQKQRIAIARA+VRNPKILLLDEATSALD +SE++VQ ALD GRT I++AHRLST++NA+VI I+ GK+ EQG+H++L+A NG+Y L
Sbjct: 794 IGIAAGVVCYIQTFFFNLAGVWLTTRMRSKTFRCIMNQEMGWFDRKENSIGALSARLSGDAASVQGAIGFPLSNIIQAFTNFICSIAIAFPYSWELALICLSTSPFMVASIVFEARFGEKSALKEKEVLEETSRIATETITQIRTVAGLRREEELIKIYDKEVERYRHQILSRLKWRGLVNSLGKSLMFF--------GYAVTLTYGGHMCADGK-IKFETIMKISNTMLYGLFILAQSLAFTPAFNAALLSANRMYEIIDRKPQIQSPESFEIQQNGNGTAYKTNVVQQGVSYRGLNFSYPSRPHIKVLQNFNLDINQGQTVALVGASGSGKSTCVQLLMRYYDPDEGKILIDQESIHHDMDLKTLRRRLGIVSQEPSLFEKSIADNIGYGDTSRQVPMQQIIEAAKMANAHEFIMSLPAQYDTVLGSKGTQLSGGQKQRIAIARAMVRNPKILLLDEATSALDFQSERVVQQALDSACSGRTCIVIAHRLSTIQNANVICVIQAGKIVEQGSHSQLLAKNGIYSKL 1312 HSP 4 Score: 301.212 bits (770), Expect = 1.378e-82 Identity = 192/560 (34.29%), Postives = 299/560 (53.39%), Query Frame = 2
Query: 2408 LFG---FLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLY--------NMSKMTN--VFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
LFG L A N+++ D ++ I + ++ S V F +LT R R F+A L+Q+I W D + R++D +IR E+LG + +C + +V +SF + W+++L ++ ++P+ V + ++ + + + E I IRTV E + Y +K K G + +A +F A +F L+LY + + T V I +I A + ++ F + +A+ + +++DR I+ S G +L+ + G ++ +V F YP R ++I+L ++ + QT+ALVGPSGCGKST I LLQRFYD + G +L+D ++ NI+ LR ++V QEP LF SI ENI +G T ++ AAKA+N HDFI+ L Y+T + EKG SGGQ+QRIAIARAL++ P+ILLLDEATSALD SEK+VQ ALDKA +GRT +V++HRLS I++A +I+ ++GK E GTH EL+ G Y +M+++ S+
Sbjct: 99 LFGGGKILTNASYEENMQELRKDSVSFGILMTLNTLLMLFSGVYYVDAFNRLALKLTVRMRREFFKATLRQEIGWHDMAKDQ--NFAVRITDNMEKIRSGIAENLGHYVEIMCDVIISVVLSFIYGWKLALAIVFYIPLTLVVNSAVAHYQGKLTGQEQSSYVRASSVVEEVIGAIRTVVAFGGERSESLRYDSLLKPALKAGKWKGAFSGLSDTVMKAMLFITGAGSFWYGANLILYYRDPSIPIDEREYTPAVVMIVISGIIVSANQISRTSPFLETFAMARGSASAILDVIDRTSLIDPLSKAGKILNY-GLKGAVEFRDVFFRYPAREDVIVLRGLNVVVEEGQTVALVGPSGCGKSTCIQLLQRFYDPVFGQVLLDGEDVRKYNIKWLRSNIAVVGQEPVLFQGSIGENIRHGKPEA--TQKEVEDAAKAANAHDFIIALHKGYDTDISEKGVQLSGGQRQRIAIARALIQQPKILLLDEATSALDYHSEKLVQAALDKACKGRTTLVVSHRLSAIRHAHRIVYIENGKAVEQGTHEELMKLEGFYHKMVTVHSY 653
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Zebrafish
Match: abcb4 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 [Source:ZFIN;Acc:ZDB-GENE-080204-52]) HSP 1 Score: 580.482 bits (1495), Expect = 2.355e-176 Identity = 298/616 (48.38%), Postives = 410/616 (66.56%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQ---FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+LF YA DI++M+ +IMS+ G PL+++VFG+ T + T + +++ I NFT ++ TL M+ ++ + + + +Y+ V F+ + + Q+ +R FF ++M+Q IGWFD +G L +L DD+ ++ GIGDK+G ++Q FI GII+ F +GWKLTLV L++SPL+ I ++ K M T K AY KAG +AEEVL SIRT+ +F G+K E+ RY +NL++A +G+RK+I +G +++ SYAL FWYG++LI G + +G +L +FF+ L+ G+GQ +P++ +F+ AR AA ++F+IID P+I+ +EEG KL+ +G I FK + F YP+R D +L G++L + GQT+ALVG+SGCGKS+ IQL+QRFYD EG V ID +I+ LNV LRE IGVVSQEPVLF +I ENIRYGR DVT +EI+QA ANA FI LP+K +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTTI+VAHRLST+RNA VI G ++G++ E GTH ELM G+Y LV+ Q
Sbjct: 43 ELFRYADSIDILLMMLGLIMSMANGAVLPLMVIVFGDMT---------------------DSFVDDTLLDNLKNITLPP--NFTFPETSNITLGEKMTTHAIYYSIMGFVVLVAAYMQVAFWTLAAGRQVKKLRKIFFHSIMKQEIGWFDVNETGQLNTRLTDDVYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKEQTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYMSYALAFWYGSTLILGGE-YTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQIIDHEPKINSFSEEGYKLDVVKGNIEFKNIHFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCGKSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYGRQDVTQDEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQAALDKVRLGRTTIVVAHRLSTIRNADVIAGFQNGEIVELGTHDELMERKGIYHSLVNMQ 634 HSP 2 Score: 537.339 bits (1383), Expect = 2.269e-161 Identity = 271/584 (46.40%), Postives = 392/584 (67.12%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANP--NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ-SH 4048
F V+ LN PE Y+ +G + A NG +QP+F+++F ++ A P N+ +Q D+ Y + AGI V++F + LQ FG +GE LT R R F AM++QD+AW+D+ N++GALTTRL+ + Q++G +G L + + ++G A+ ISF + WQ++L++L+ VPI+ V G +Q++++ G + DK LE++GKIA E IEN+RTV +LTRE F Y+E + KKAH G + +QA I+ AYA F +L+ + VF+ A+++GAM +G++ + PN+ K K++A+H+ L++R P I++ S DG D G++ E+V F YP RP++ +L + + QT+ALVG SGCGKST I LL+RFYD G +++D+N K +NI LR IVSQEP LF S+ ENIAYGD+++ ++I+ AAKA+NIH FI NLP +Y+T+ G+KG+ SGGQKQRIAIARA+LRNP++LLLDEATSALDTESEKIVQDALDKA +GRTC+++AHRLSTIQNAD I V ++G + E GTH +LLS+ G Y +++ Q SH
Sbjct: 696 FLTVLKLNYPEWPYMVVGILCATINGGMQPAFAVIFSKIIAVFAEPDQNLVRQRCDL--YSLLFAGIGVLSFFTLFLQGFCFGKAGELLTMRLRFKAFNAMMRQDLAWYDDTKNSVGALTTRLAADTAQVQGATGVRLATLAQNVANLGTAIVISFVYGWQLTLLILSIVPIMAVAGAIQMKLLAGHALKDKKELEQAGKIATEAIENVRTVVSLTRESKFESLYEENLIVPYKNAKKKAHVFGLTFSFSQAMIYFAYAGCFKFGSWLIEQKLMTFEGVFLVISAVVYGAMAVGEANSFTPNYAKAKMSASHVLMLINRAPAIDNSSEDGDKPD--KFEGNVGFEHVYFKYPSRPDVPVLQGLKLRVKKGQTLALVGSSGCGKSTTIQLLERFYDPQQGRVMLDDNDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSREVDQEEIVEAAKAANIHSFIENLPQRYQTQAGDKGTQLSGGQKQRIAIARAILRNPKVLLLDEATSALDTESEKIVQDALDKASKGRTCIIVAHRLSTIQNADCIAVVQNGVVVEQGTHQQLLSQQGAYYTLVTSQMSH 1275 HSP 3 Score: 414.461 bits (1064), Expect = 1.136e-119 Identity = 228/518 (44.02%), Postives = 326/518 (62.93%), Query Frame = 1
Query: 610 FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
FAGI + F ++ F + +R F A+MRQ++ W+D +NS GALT +L D V G ++ + Q A I+++FV GW+LTL+ LSI P++ + + K + K + +AG IA E + ++RT+VS R K+E + Y ENL K +K+ + G +++ +YA F +G+ LI + G + +V + + +G+A ++ +A+ +A+ + +I+R P ID +E+GDK + F+G + F++V F YP+R D P+L GL L ++ GQT+ALVG+SGCGKS+ IQL++RFYD +G V++DD + K LN+ WLR QIG+VSQEPVLF CS+ ENI YG +V EEI +A AN +FI LP + T GD+GTQLSGGQKQRIAIARA++RNPK+LLLDEATSALD+ESEKIVQDALDK +GRT IIVAHRLST++NA I +++G V EQGTH +L++ G Y LV Q
Sbjct: 757 FAGIGVLSFFTLFLQGFCFGKAGELLTMRLRFKAFNAMMRQDLAWYDDTKNSVGALTTRLAADTAQVQGATGVRLATLAQNVANLGTAIVISFVYGWQLTLLILSIVPIMAVAGAIQMKLLAGHALKDKKELEQAGKIATEAIENVRTVVSLTRESKFE-SLYEENLIVPYKNAKKKAHVFGLTFSFSQAMIYFAYAGCFKFGSWLIEQKLMTFEG-VFLVISAVVYGAMAVGEANSFTPNYAKAKMSASHVLMLINRAPAIDNSSEDGDKPDKFEGNVGFEHVYFKYPSRPDVPVLQGLKLRVKKGQTLALVGSSGCGKSTTIQLLERFYDPQQGRVMLDDNDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSREVDQEEIVEAAKAANIHSFIENLPQRYQTQAGDKGTQLSGGQKQRIAIARAILRNPKVLLLDEATSALDTESEKIVQDALDKASKGRTCIIVAHRLSTIQNADCIAVVQNGVVVEQGTHQQLLSQQGAYYTLVTSQ 1272 HSP 4 Score: 391.349 bits (1004), Expect = 4.528e-112 Identity = 228/591 (38.58%), Postives = 349/591 (59.05%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFG-----------------------FLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 4051
+ LG + ++ NGAV P I+FG F P +N + ++ + T+ I + + V V++ +Q + + + K+ R I F +++KQ+I WFD N G L TRL+D+ +I G+ LG +I + + + I F W+++LV+LA P++ + + ++M F++ ++ A ++G +A E + +IRTV + I+ Y + ++ +KA G T I+ +YA F L+L + + F A++ GA LG++ F + AA +F+++D P INSFS +G LDV + G+I+ +N+ F YP R ++ +LN ++ + S QTIALVG SGCGKST I LLQRFYD G++ ID + I+ +N++ LR+L +VSQEP LF+ +I ENI YG + T D+I AA+ +N ++FI+ LPD++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD ESE IVQ ALDK + GRT +V+AHRLSTI+NAD I F++G+I E GTH EL+ + GIY ++++Q K
Sbjct: 56 MMLGLIMSMANGAVLPLMVIVFGDMTDSFVDDTLLDNLKNITLPPNFTFPETSNITLGEK---MTTHAIYYSIMGFVVLVAAYMQVAFWTLAAGRQVKKLRKIFFHSIMKQEIGWFD--VNETGQLNTRLTDDVYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKEQTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYMSYALAFWYGSTLILGGEYTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQIIDHEPKINSFSEEGYKLDV--VKGNIEFKNIHFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCGKSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYG--RQDVTQDEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQAALDKVRLGRTTIVVAHRLSTIRNADVIAGFQNGEIVELGTHDELMERKGIYHSLVNMQMFK 637
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Zebrafish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414]) HSP 1 Score: 560.451 bits (1443), Expect = 1.053e-168 Identity = 297/629 (47.22%), Postives = 409/629 (65.02%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSG---------MSQQ---FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASL-IREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G +LF YATC ++ +M+ ++ + GV PL+ +VFG T + + T LT N TS NFT + +N T ++G M++Q F GI A+ ++ V F++T+ Q IR +F A++ Q + WFD G L +L DDINT++ G+GDKI +Q++ FI G+++ FV GWKLTLV L++SPL+ + SK + LT K AY KAG +AEE+L +IRT+V+F G+K V +Y +NL EA G++K+I T +GL ++F +YAL FWYG L + E + +G +L VFFS ++ T +GQ P++ + +AR AA E+++ ID ID ++EG K + +G I FK + F YP+R D IL G+SL + G+T+ALVGASGCGKS+ IQL+QRFYD + GEV +D +I+ LNV WLRE +G+VSQEPVLFG +I ENIRYGR D TD +I+QA+ ANA FI+ LP+KL+T+VG+RG QLSGGQKQRIAIARALV+NPKILLLDEATSALD++SE IVQ ALDK GRTTI++AHRLST+R+A +I G +G+V EQG+H ELMA G+Y LV +Q
Sbjct: 68 GFFQLFRYATCPEVFLMLIGLLCAAAHGVALPLMCVVFGQMTDS-----FVQSGQTYNLTGFNGNFTS----------------NFTFTLNNTSTCLAGSPEIGIEPKMTKQAYFFIGIGGAVLVLGTFQVMLFLLTAAKQTKRIRQKYFHAILHQQMSWFDTHPIGELNIRLTDDINTINDGLGDKIAVFVQFFCSFISGLVIGFVFGWKLTLVILAVSPLLAGSAAVWSKILASLTSKELTAYAKAGAVAEEILVAIRTVVAFNGQKKAVEKYEKNLVEAKDFGVKKAISTNVSMGLTQFIVFATYALAFWYGTKLSVDEPENYTIGRVLTVFFSVMIGTFSLGQGAPNLEAIAKARGAAYEVYKTIDMPRPIDSSSKEGHKPDRVRGDIEFKNINFNYPSRKDVTILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDSGEVTLDGHDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDADIEQAIKEANAYDFISKLPDKLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSESIVQAALDKARAGRTTIVIAHRLSTIRSADIIAGFSEGRVVEQGSHRELMAKKGVYYSLVTQQ 675 HSP 2 Score: 504.597 bits (1298), Expect = 1.874e-149 Identity = 257/579 (44.39%), Postives = 377/579 (65.11%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F K++ LN P+ Y+ +G +++ GAV P +ILF ++ A P+ E + + + I VVAF++ Q FG SGE LT R RS F+A+++Q+I WFD+ +N +G LTT+L+ +A+ ++G +G LG IC++ AV ++F FCWQ++L++LA VP + +Q+R G ++ D+ ALE SGKI+ E +EN +TV LTRE F + +++ KA G + L QA + AA F +L+ + ++ NVF+ F ++F AMN+G+S + AP+F K K AA + +LL++ P I+ + G SG+I ++V+FSYP RPN+ +L ++ ++ QT+ALVG SGCGKST I LL+RFYD G +L+D K +N+ LR +VSQEP LF +I ENI YGD+++ T ++I AAK +NIH+FI+ LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P++LLLDEATSALDTESEKIVQ ALD+A+ GRTC+VIAHRL+TIQNAD I+V ++GK+ E GTH +L++K Y +++ Q
Sbjct: 758 FTKILALNKPDWPYLVVGTFASLVGGAVYPCVAILFAKIIGVFAEPDPEVKRQKTMMFSLLYLLIGVVAFLTYFFQGFMFGKSGELLTMRLRSQAFKAIVRQEIGWFDDNNNAVGILTTKLATDASLVKGAAGSRLGLATNTICALLIAVIVAFVFCWQLTLLILACVPFLTGANFIQLRATAGHTSKDQSALEMSGKISTETVENFKTVVALTREDVFFHKFIDSLSTPYKASLCKAPIYGITFALAQAIPYLVNAAIFRFGAWLIAHCYTEYENVFLVFSVIVFAAMNIGQSSSFAPDFAKAKAAAGRIIQLLEKKPEIDIYDESGE--RPSTFSGNIDFKDVQFSYPTRPNVKVLQGLNVSVRQGQTLALVGSSGCGKSTTIQLLERFYDPAGGQVLVDGRDSKSVNLAWLRTQMGLVSQEPILFDCTISENIQYGDNSRTVTQEEIEEAAKKANIHNFILTLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKLLLLDEATSALDTESEKIVQAALDEARLGRTCIVIAHRLTTIQNADIIVVVQNGKVVEQGTHAQLMAKQEAYFALVNAQ 1334 HSP 3 Score: 390.963 bits (1003), Expect = 1.609e-111 Identity = 215/488 (44.06%), Postives = 310/488 (63.52%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+R+ F+A++RQ IGWFD N+ G LT KL D + V G ++G + +I+AFV W+LTL+ L+ P + + + T K A +G I+ E + + +T+V+ E ++ ++L K + K+ + G L + + A F +GA LI ++ +VF + A IGQ++ F +A++AA I +++++ PEID +E G++ + F G I FK V+F+YPTR + +L GL++S++ GQT+ALVG+SGCGKS+ IQL++RFYD G+VL+D + K +N+ WLR Q+G+VSQEP+LF C+I ENI+YG VT EEI++A AN FI LP+K +T VGD+GTQLSGGQKQRIAIARALVR PK+LLLDEATSALD+ESEKIVQ ALD+ GRT I++AHRL+T++NA +I +++GKV EQGTHA+LMA Y LV+ Q
Sbjct: 848 LRSQAFKAIVRQEIGWFDDNNNAVGILTTKLATDASLVKGAAGSRLGLATNTICALLIAVIVAFVFCWQLTLLILACVPFLTGANFIQLRATAGHTSKDQSALEMSGKISTETVENFKTVVALTREDVFFHKFIDSLSTPYKASLCKAPIYGITFALAQAIPYLVNAAIFRFGAWLIAHCYT-EYENVFLVFSVIVFAAMNIGQSSSFAPDFAKAKAAAGRIIQLLEKKPEIDIYDESGERPSTFSGNIDFKDVQFSYPTRPNVKVLQGLNVSVRQGQTLALVGSSGCGKSTTIQLLERFYDPAGGQVLVDGRDSKSVNLAWLRTQMGLVSQEPILFDCTISENIQYGDNSRTVTQEEIEEAAKKANIHNFILTLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKLLLLDEATSALDTESEKIVQAALDEARLGRTCIVIAHRLTTIQNADIIVVVQNGKVVEQGTHAQLMAKQEAYFALVNAQ 1334 HSP 4 Score: 351.288 bits (900), Expect = 1.833e-98 Identity = 211/525 (40.19%), Postives = 310/525 (59.05%), Query Frame = 2
Query: 2495 GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL-----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNF---GKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
GI V Q + F + + TKR R F A+L Q ++WFD ++ IG L RL+D+ I G+ + + CS + + I F F W+++LV+LA P++ + +I+ ++ + A ++G +A E + IRTV + ++ Y++ + + KKA + GLTQ +F YA F L + Y + ++ VF S ++ G +LG+ APN K + AA +++ +D PI+S S +G D + GDI+ +N+ F+YP R ++ IL +S + +TIALVG SGCGKST I LLQRFYD +G + +D + I+ +N++ LR+ IVSQEP LF +I ENI YG TD I A K +N +DFI LPD+ T VGE+G+ SGGQKQRIAIARAL++NP+ILLLDEATSALDT+SE IVQ ALDKA+ GRT +VIAHRLSTI++AD I F +G++ E G+H EL++K G+Y +++ Q+
Sbjct: 164 GIGGAVLVLGTFQVMLFLLTAAKQTKRIRQKYFHAILHQQMSWFD--THPIGELNIRLTDDINTINDGLGDKIAVFVQFFCSFISGLVIGFVFGWKLTLVILAVSPLLAGSAAVWSKILASLTSKELTAYAKAGAVAEEILVAIRTVVAFNGQKKAVEKYEKNLVEAKDFGVKKAISTNVSMGLTQFIVFATYALAFWYGTKLSVDEPENYTIGRVLTVFFS---VMIGTFSLGQG---APNLEAIAKARGAAYEVYKTIDMPRPIDSSSKEGHKPD--RVRGDIEFKNINFNYPSRKDVTILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDSGEVTLDGHDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDA--TDADIEQAIKEANAYDFISKLPDKLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSESIVQAALDKARAGRTTIVIAHRLSTIRSADIIAGFSEGRVVEQGSHRELMAKKGVYYSLVTQQT 676
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Zebrafish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414]) HSP 1 Score: 560.451 bits (1443), Expect = 1.292e-168 Identity = 297/629 (47.22%), Postives = 409/629 (65.02%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSG---------MSQQ---FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASL-IREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G +LF YATC ++ +M+ ++ + GV PL+ +VFG T + + T LT N TS NFT + +N T ++G M++Q F GI A+ ++ V F++T+ Q IR +F A++ Q + WFD G L +L DDINT++ G+GDKI +Q++ FI G+++ FV GWKLTLV L++SPL+ + SK + LT K AY KAG +AEE+L +IRT+V+F G+K V +Y +NL EA G++K+I T +GL ++F +YAL FWYG L + E + +G +L VFFS ++ T +GQ P++ + +AR AA E+++ ID ID ++EG K + +G I FK + F YP+R D IL G+SL + G+T+ALVGASGCGKS+ IQL+QRFYD + GEV +D +I+ LNV WLRE +G+VSQEPVLFG +I ENIRYGR D TD +I+QA+ ANA FI+ LP+KL+T+VG+RG QLSGGQKQRIAIARALV+NPKILLLDEATSALD++SE IVQ ALDK GRTTI++AHRLST+R+A +I G +G+V EQG+H ELMA G+Y LV +Q
Sbjct: 80 GFFQLFRYATCPEVFLMLIGLLCAAAHGVALPLMCVVFGQMTDS-----FVQSGQTYNLTGFNGNFTS----------------NFTFTLNNTSTCLAGSPEIGIEPKMTKQAYFFIGIGGAVLVLGTFQVMLFLLTAAKQTKRIRQKYFHAILHQQMSWFDTHPIGELNIRLTDDINTINDGLGDKIAVFVQFFCSFISGLVIGFVFGWKLTLVILAVSPLLAGSAAVWSKILASLTSKELTAYAKAGAVAEEILVAIRTVVAFNGQKKAVEKYEKNLVEAKDFGVKKAISTNVSMGLTQFIVFATYALAFWYGTKLSVDEPENYTIGRVLTVFFSVMIGTFSLGQGAPNLEAIAKARGAAYEVYKTIDMPRPIDSSSKEGHKPDRVRGDIEFKNINFNYPSRKDVTILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDSGEVTLDGHDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDADIEQAIKEANAYDFISKLPDKLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSESIVQAALDKARAGRTTIVIAHRLSTIRSADIIAGFSEGRVVEQGSHRELMAKKGVYYSLVTQQ 687 HSP 2 Score: 504.597 bits (1298), Expect = 2.371e-149 Identity = 257/579 (44.39%), Postives = 377/579 (65.11%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F K++ LN P+ Y+ +G +++ GAV P +ILF ++ A P+ E + + + I VVAF++ Q FG SGE LT R RS F+A+++Q+I WFD+ +N +G LTT+L+ +A+ ++G +G LG IC++ AV ++F FCWQ++L++LA VP + +Q+R G ++ D+ ALE SGKI+ E +EN +TV LTRE F + +++ KA G + L QA + AA F +L+ + ++ NVF+ F ++F AMN+G+S + AP+F K K AA + +LL++ P I+ + G SG+I ++V+FSYP RPN+ +L ++ ++ QT+ALVG SGCGKST I LL+RFYD G +L+D K +N+ LR +VSQEP LF +I ENI YGD+++ T ++I AAK +NIH+FI+ LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P++LLLDEATSALDTESEKIVQ ALD+A+ GRTC+VIAHRL+TIQNAD I+V ++GK+ E GTH +L++K Y +++ Q
Sbjct: 770 FTKILALNKPDWPYLVVGTFASLVGGAVYPCVAILFAKIIGVFAEPDPEVKRQKTMMFSLLYLLIGVVAFLTYFFQGFMFGKSGELLTMRLRSQAFKAIVRQEIGWFDDNNNAVGILTTKLATDASLVKGAAGSRLGLATNTICALLIAVIVAFVFCWQLTLLILACVPFLTGANFIQLRATAGHTSKDQSALEMSGKISTETVENFKTVVALTREDVFFHKFIDSLSTPYKASLCKAPIYGITFALAQAIPYLVNAAIFRFGAWLIAHCYTEYENVFLVFSVIVFAAMNIGQSSSFAPDFAKAKAAAGRIIQLLEKKPEIDIYDESGE--RPSTFSGNIDFKDVQFSYPTRPNVKVLQGLNVSVRQGQTLALVGSSGCGKSTTIQLLERFYDPAGGQVLVDGRDSKSVNLAWLRTQMGLVSQEPILFDCTISENIQYGDNSRTVTQEEIEEAAKKANIHNFILTLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKLLLLDEATSALDTESEKIVQAALDEARLGRTCIVIAHRLTTIQNADIIVVVQNGKVVEQGTHAQLMAKQEAYFALVNAQ 1346 HSP 3 Score: 391.349 bits (1004), Expect = 1.326e-111 Identity = 215/488 (44.06%), Postives = 310/488 (63.52%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+R+ F+A++RQ IGWFD N+ G LT KL D + V G ++G + +I+AFV W+LTL+ L+ P + + + T K A +G I+ E + + +T+V+ E ++ ++L K + K+ + G L + + A F +GA LI ++ +VF + A IGQ++ F +A++AA I +++++ PEID +E G++ + F G I FK V+F+YPTR + +L GL++S++ GQT+ALVG+SGCGKS+ IQL++RFYD G+VL+D + K +N+ WLR Q+G+VSQEP+LF C+I ENI+YG VT EEI++A AN FI LP+K +T VGD+GTQLSGGQKQRIAIARALVR PK+LLLDEATSALD+ESEKIVQ ALD+ GRT I++AHRL+T++NA +I +++GKV EQGTHA+LMA Y LV+ Q
Sbjct: 860 LRSQAFKAIVRQEIGWFDDNNNAVGILTTKLATDASLVKGAAGSRLGLATNTICALLIAVIVAFVFCWQLTLLILACVPFLTGANFIQLRATAGHTSKDQSALEMSGKISTETVENFKTVVALTREDVFFHKFIDSLSTPYKASLCKAPIYGITFALAQAIPYLVNAAIFRFGAWLIAHCYT-EYENVFLVFSVIVFAAMNIGQSSSFAPDFAKAKAAAGRIIQLLEKKPEIDIYDESGERPSTFSGNIDFKDVQFSYPTRPNVKVLQGLNVSVRQGQTLALVGSSGCGKSTTIQLLERFYDPAGGQVLVDGRDSKSVNLAWLRTQMGLVSQEPILFDCTISENIQYGDNSRTVTQEEIEEAAKKANIHNFILTLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKLLLLDEATSALDTESEKIVQAALDEARLGRTCIVIAHRLTTIQNADIIVVVQNGKVVEQGTHAQLMAKQEAYFALVNAQ 1346 HSP 4 Score: 350.903 bits (899), Expect = 2.519e-98 Identity = 211/525 (40.19%), Postives = 310/525 (59.05%), Query Frame = 2
Query: 2495 GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL-----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNF---GKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
GI V Q + F + + TKR R F A+L Q ++WFD ++ IG L RL+D+ I G+ + + CS + + I F F W+++LV+LA P++ + +I+ ++ + A ++G +A E + IRTV + ++ Y++ + + KKA + GLTQ +F YA F L + Y + ++ VF S ++ G +LG+ APN K + AA +++ +D PI+S S +G D + GDI+ +N+ F+YP R ++ IL +S + +TIALVG SGCGKST I LLQRFYD +G + +D + I+ +N++ LR+ IVSQEP LF +I ENI YG TD I A K +N +DFI LPD+ T VGE+G+ SGGQKQRIAIARAL++NP+ILLLDEATSALDT+SE IVQ ALDKA+ GRT +VIAHRLSTI++AD I F +G++ E G+H EL++K G+Y +++ Q+
Sbjct: 176 GIGGAVLVLGTFQVMLFLLTAAKQTKRIRQKYFHAILHQQMSWFD--THPIGELNIRLTDDINTINDGLGDKIAVFVQFFCSFISGLVIGFVFGWKLTLVILAVSPLLAGSAAVWSKILASLTSKELTAYAKAGAVAEEILVAIRTVVAFNGQKKAVEKYEKNLVEAKDFGVKKAISTNVSMGLTQFIVFATYALAFWYGTKLSVDEPENYTIGRVLTVFFS---VMIGTFSLGQG---APNLEAIAKARGAAYEVYKTIDMPRPIDSSSKEGHKPD--RVRGDIEFKNINFNYPSRKDVTILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDSGEVTLDGHDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDA--TDADIEQAIKEANAYDFISKLPDKLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSESIVQAALDKARAGRTTIVIAHRLSTIRSADIIAGFSEGRVVEQGSHRELMAKKGVYYSLVTQQT 688
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Zebrafish
Match: abcb11a (ATP-binding cassette, sub-family B (MDR/TAP), member 11a [Source:ZFIN;Acc:ZDB-GENE-050517-13]) HSP 1 Score: 506.523 bits (1303), Expect = 9.854e-151 Identity = 254/575 (44.17%), Postives = 379/575 (65.91%), Query Frame = 2
Query: 2312 KVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMIS 4036
+++ N PE Y+ LG + A NG++ P +++LF +L + P+ + Q I I I VV+F S LQ F SGE LT+R R F+AMLKQ+I WFD+P N+ GALTTRL+ A+ ++G +G +G ++ ++ ++GA+ I++ F W++SLVV F+P+I + G+ Q +++ G +N DK ALE +G+++ E + NIRT++ L +E F+ +++ ++ KKA+ G + + IF AYAA+F GYL+ + + VF AL+ A LG++ + P++ K KI+AA +F+LLDRVP IN T+G + + G ++ + +F+YP RP++ +L + +++ QT+A VG SGCGKST + LL+RFYD G +LID I++ LR IVSQEP LF SI ENI YGD+++ + ++II AAK + +HDF++ LPD+YET+VG +GS S GQKQRIAIARA++RNP+ILLLDEATSALDTESEK VQ ALD+A++GRTC+VIAHRLSTIQ+AD I V G++ E GTH EL++K Y ++++
Sbjct: 699 RILKYNRPEWPYMLLGSIGAAINGSLNPMYALLFSQILGTFSIPDPDDQRRQINGICILFVVIGVVSFFSQFLQGYSFAKSGELLTRRLRKFGFQAMLKQEIGWFDDPMNSPGALTTRLATNASMVQGATGSQIGMIVNSLTNIGASFIIAYYFSWKLSLVVTCFLPLIGLSGVFQSKMLTGLANEDKTALEAAGQVSSEAMSNIRTIAGLAKEKHFVAQFEKQLQAPYKAAKKKAYVYGICFAFARCVIFMAYAASFRYGGYLVSHEGLQYMMVFRVISALVTSATALGRASSFTPDYAKAKISAAQLFQLLDRVPKINVSKTEGQSWN--DFKGKVEFKGCRFTYPSRPDVQVLRGLVVSVHPGQTLAFVGSSGCGKSTSVQLLERFYDPDEGQVLIDGRPSDSISVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSRTVSMEEIIDAAKKAYLHDFVMTLPDKYETQVGAQGSQLSRGQKQRIAIARAIVRNPKILLLDEATSALDTESEKTVQAALDEARQGRTCIVIAHRLSTIQSADIIAVMSQGEVIEKGTHDELMAKKAAYYKLVT 1271 HSP 2 Score: 474.167 bits (1219), Expect = 9.193e-140 Identity = 268/612 (43.79%), Postives = 387/612 (63.24%), Query Frame = 1
Query: 316 FSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSS-NIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
F ++T +++MM+ S+ G PL++LV+G T+ Y+ + LT N+ ++T I M + N T+ +I M+ + + GI + + I+S+ +TF+V + QI IR +FR +M IGWFD + G L ++ DDIN ++ I D++ ++ + FI G ++ F+ GWKLTLV +++SPL+ + +++ + +LT + +AY KAG +A+EVL SIRT+ +F GE E RY NL +A + GI+K ++ G G + ++F YAL FW+G+ L+ E G ++ VFF L+ +GQA+P + +F R+AA IF+ IDR PEID ++EG L+ +G I F V F YP+R + IL L++ ++ G+T A VG SG GK++ IQL+QRFYD +EG V +D +I+ LN+ WLR IGVV QEPVLF +I ENIRYGR VT +EI +A ANA FI +LP DTLVG+ G Q+SGGQKQRIAIARALVRNP+ILLLD ATSALD+ESE +VQ+ALDK +GRTTI +AHRLST+RNA VI G + G+ E+GTH++L+ G+Y LV Q
Sbjct: 1 FRFSTWREVLMMVVGSFCSLVHGAATPLMLLVYGMMTNTFVEYEVEILELT----DPNKTCINNT-ISWMNGSAVQRPDNTTIYCGVDIEAEMTNFALYYIGIGVGVLILSFFQITFWVSAAARQIQRIRKTYFRKIMCMEIGWFDCNSVGELNTRMSDDINKINNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVIAVSPLLGLAAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEHKEAERYDRNLVQAQEWGIKKGMIIGVFQGYLWCIIFLCYALAFWFGSKLVIETQELTPGGLVQVFFGVLIGAMNLGQASPCLEAFASGRAAAKSIFDTIDREPEIDCFSDEGHTLDKVKGDIEFHSVNFNYPSRPEVKILDDLNIVVKAGETTAFVGPSGSGKTTTIQLIQRFYDPSEGMVSLDGHDIRSLNIQWLRSLIGVVEQEPVLFATTIAENIRYGRAGVTMQEIIEAAKQANAYNFIMSLPQTFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKARQGRTTISIAHRLSTIRNADVIVGFEHGRAVERGTHSQLLDKKGVYFTLVTLQ 607 HSP 3 Score: 382.874 bits (982), Expect = 3.472e-109 Identity = 234/588 (39.80%), Postives = 343/588 (58.33%), Query Frame = 1
Query: 391 WPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLI-REGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
WP ++L S A +N + L SQ I + SIPD ++ R ++G+ F I + F ++ F + + +R F+A+++Q IGWFD + GALT +L + + V G +IG ++ I+A+ WKL+LV PLI + + SK + L + A AG ++ E + +IRTI EK+ VA++ + L K +K+ + G ++F +YA F YG L+ EG +++ + V + + + T +G+A+ + +A+ +AA++F+++DRVP+I+ EG NDF+GK+ FK +FTYP+R D +L GL +S+ PGQT+A VG+SGCGKS+ +QL++RFYD +EG+VLID ++V +LR QIG+VSQEPVLF CSI ENI+YG V+ EEI A A F+ LP+K +T VG +G+QLS GQKQRIAIARA+VRNPKILLLDEATSALD+ESEK VQ ALD+ +GRT I++AHRLST+++A +I + G+V E+GTH ELMA Y LV
Sbjct: 708 WPYMLL-----GSIGAAINGSLNPMYALLFSQ---ILGTFSIPDPDDQRRQ---------------INGICILFVVIGVVSFFSQFLQGYSFAKSGELLTRRLRKFGFQAMLKQEIGWFDDPMNSPGALTTRLATNASMVQGATGSQIGMIVNSLTNIGASFIIAYYFSWKLSLVVTCFLPLIGLSGVFQSKMLTGLANEDKTALEAAGQVSSEAMSNIRTIAGLAKEKHFVAQFEKQLQAPYKAAKKKAYVYGICFAFARCVIFMAYAASFRYGGYLVSHEGLQYMM--VFRVISALVTSATALGRASSFTPDYAKAKISAAQLFQLLDRVPKINVSKTEGQSWNDFKGKVEFKGCRFTYPSRPDVQVLRGLVVSVHPGQTLAFVGSSGCGKSTSVQLLERFYDPDEGQVLIDGRPSDSISVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSRTVSMEEIIDAAKKAYLHDFVMTLPDKYETQVGAQGSQLSRGQKQRIAIARAIVRNPKILLLDEATSALDTESEKTVQAALDEARQGRTCIVIAHRLSTIQSADIIAVMSQGEVIEKGTHDELMAKKAAYYKLV 1270 HSP 4 Score: 334.339 bits (856), Expect = 2.704e-93 Identity = 212/532 (39.85%), Postives = 311/532 (58.46%), Query Frame = 2
Query: 2462 ADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTN---VFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
A++ + + GI V + S Q + ++ + +R R F ++ +I WFD N++G L TR+SD+ +I + + I I + + F W+++LVV+A P++ + L + + + A ++G +A E + +IRTV+ EH + Y + + KK G G IF YA F G L+ ++T V + FG LI GAMNLG++ F G+ AA +F+ +DR P I+ FS +G LD + GDI+ +V F+YP RP + IL+D++ + + +T A VGPSG GK+T I L+QRFYD G + +D + I+ +NIQ LR L +V QEP LF+ +I ENI YG + T +II AAK +N ++FI++LP ++T VGE G SGGQKQRIAIARAL+RNPRILLLD ATSALD ESE +VQ+ALDKA++GRT + IAHRLSTI+NAD I+ F+ G+ E GTH +LL K G+Y +++LQ+
Sbjct: 86 AEMTNFALYYIGIGVGVLILSFFQITFWVSAAARQIQRIRKTYFRKIMCMEIGWFD--CNSVGELNTRMSDDINKINNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVIAVSPLLGLAAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEHKEAERYDRNLVQAQEWGIKKGMIIGVFQGYLWCIIFLCYALAFWF-GSKLVIETQELTPGGLVQVFFGVLI-GAMNLGQASPCLEAFASGRAAAKSIFDTIDREPEIDCFSDEGHTLD--KVKGDIEFHSVNFNYPSRPEVKILDDLNIVVKAGETTAFVGPSGSGKTTTIQLIQRFYDPSEGMVSLDGHDIRSLNIQWLRSLIGVVEQEPVLFATTIAENIRYGRAG--VTMQEIIEAAKQANAYNFIMSLPQTFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKARQGRTTISIAHRLSTIRNADVIVGFEHGRAVERGTHSQLLDKKGVYFTLVTLQNQ 609
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Zebrafish
Match: abcb11a (ATP-binding cassette, sub-family B (MDR/TAP), member 11a [Source:ZFIN;Acc:ZDB-GENE-050517-13]) HSP 1 Score: 506.908 bits (1304), Expect = 1.995e-150 Identity = 254/575 (44.17%), Postives = 379/575 (65.91%), Query Frame = 2
Query: 2312 KVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMIS 4036
+++ N PE Y+ LG + A NG++ P +++LF +L + P+ + Q I I I VV+F S LQ F SGE LT+R R F+AMLKQ+I WFD+P N+ GALTTRL+ A+ ++G +G +G ++ ++ ++GA+ I++ F W++SLVV F+P+I + G+ Q +++ G +N DK ALE +G+++ E + NIRT++ L +E F+ +++ ++ KKA+ G + + IF AYAA+F GYL+ + + VF AL+ A LG++ + P++ K KI+AA +F+LLDRVP IN T+G + + G ++ + +F+YP RP++ +L + +++ QT+A VG SGCGKST + LL+RFYD G +LID I++ LR IVSQEP LF SI ENI YGD+++ + ++II AAK + +HDF++ LPD+YET+VG +GS S GQKQRIAIARA++RNP+ILLLDEATSALDTESEK VQ ALD+A++GRTC+VIAHRLSTIQ+AD I V G++ E GTH EL++K Y ++++
Sbjct: 742 RILKYNRPEWPYMLLGSIGAAINGSLNPMYALLFSQILGTFSIPDPDDQRRQINGICILFVVIGVVSFFSQFLQGYSFAKSGELLTRRLRKFGFQAMLKQEIGWFDDPMNSPGALTTRLATNASMVQGATGSQIGMIVNSLTNIGASFIIAYYFSWKLSLVVTCFLPLIGLSGVFQSKMLTGLANEDKTALEAAGQVSSEAMSNIRTIAGLAKEKHFVAQFEKQLQAPYKAAKKKAYVYGICFAFARCVIFMAYAASFRYGGYLVSHEGLQYMMVFRVISALVTSATALGRASSFTPDYAKAKISAAQLFQLLDRVPKINVSKTEGQSWN--DFKGKVEFKGCRFTYPSRPDVQVLRGLVVSVHPGQTLAFVGSSGCGKSTSVQLLERFYDPDEGQVLIDGRPSDSISVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSRTVSMEEIIDAAKKAYLHDFVMTLPDKYETQVGAQGSQLSRGQKQRIAIARAIVRNPKILLLDEATSALDTESEKTVQAALDEARQGRTCIVIAHRLSTIQSADIIAVMSQGEVIEKGTHDELMAKKAAYYKLVT 1314 HSP 2 Score: 477.248 bits (1227), Expect = 2.418e-140 Identity = 270/617 (43.76%), Postives = 390/617 (63.21%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSS-NIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G +LF ++T +++MM+ S+ G PL++LV+G T+ Y+ + LT N+ ++T I M + N T+ +I M+ + + GI + + I+S+ +TF+V + QI IR +FR +M IGWFD + G L ++ DDIN ++ I D++ ++ + FI G ++ F+ GWKLTLV +++SPL+ + +++ + +LT + +AY KAG +A+EVL SIRT+ +F GE E RY NL +A + GI+K ++ G G + ++F YAL FW+G+ L+ E G ++ VFF L+ +GQA+P + +F R+AA IF+ IDR PEID ++EG L+ +G I F V F YP+R + IL L++ ++ G+T A VG SG GK++ IQL+QRFYD +EG V +D +I+ LN+ WLR IGVV QEPVLF +I ENIRYGR VT +EI +A ANA FI +LP DTLVG+ G Q+SGGQKQRIAIARALVRNP+ILLLD ATSALD+ESE +VQ+ALDK +GRTTI +AHRLST+RNA VI G + G+ E+GTH++L+ G+Y LV Q
Sbjct: 39 GFFQLFRFSTWREVLMMVVGSFCSLVHGAATPLMLLVYGMMTNTFVEYEVEILELT----DPNKTCINNT-ISWMNGSAVQRPDNTTIYCGVDIEAEMTNFALYYIGIGVGVLILSFFQITFWVSAAARQIQRIRKTYFRKIMCMEIGWFDCNSVGELNTRMSDDINKINNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVIAVSPLLGLAAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEHKEAERYDRNLVQAQEWGIKKGMIIGVFQGYLWCIIFLCYALAFWFGSKLVIETQELTPGGLVQVFFGVLIGAMNLGQASPCLEAFASGRAAAKSIFDTIDREPEIDCFSDEGHTLDKVKGDIEFHSVNFNYPSRPEVKILDDLNIVVKAGETTAFVGPSGSGKTTTIQLIQRFYDPSEGMVSLDGHDIRSLNIQWLRSLIGVVEQEPVLFATTIAENIRYGRAGVTMQEIIEAAKQANAYNFIMSLPQTFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKARQGRTTISIAHRLSTIRNADVIVGFEHGRAVERGTHSQLLDKKGVYFTLVTLQ 650 HSP 3 Score: 382.874 bits (982), Expect = 5.481e-109 Identity = 234/588 (39.80%), Postives = 343/588 (58.33%), Query Frame = 1
Query: 391 WPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLI-REGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
WP ++L S A +N + L SQ I + SIPD ++ R ++G+ F I + F ++ F + + +R F+A+++Q IGWFD + GALT +L + + V G +IG ++ I+A+ WKL+LV PLI + + SK + L + A AG ++ E + +IRTI EK+ VA++ + L K +K+ + G ++F +YA F YG L+ EG +++ + V + + + T +G+A+ + +A+ +AA++F+++DRVP+I+ EG NDF+GK+ FK +FTYP+R D +L GL +S+ PGQT+A VG+SGCGKS+ +QL++RFYD +EG+VLID ++V +LR QIG+VSQEPVLF CSI ENI+YG V+ EEI A A F+ LP+K +T VG +G+QLS GQKQRIAIARA+VRNPKILLLDEATSALD+ESEK VQ ALD+ +GRT I++AHRLST+++A +I + G+V E+GTH ELMA Y LV
Sbjct: 751 WPYMLL-----GSIGAAINGSLNPMYALLFSQ---ILGTFSIPDPDDQRRQ---------------INGICILFVVIGVVSFFSQFLQGYSFAKSGELLTRRLRKFGFQAMLKQEIGWFDDPMNSPGALTTRLATNASMVQGATGSQIGMIVNSLTNIGASFIIAYYFSWKLSLVVTCFLPLIGLSGVFQSKMLTGLANEDKTALEAAGQVSSEAMSNIRTIAGLAKEKHFVAQFEKQLQAPYKAAKKKAYVYGICFAFARCVIFMAYAASFRYGGYLVSHEGLQYMM--VFRVISALVTSATALGRASSFTPDYAKAKISAAQLFQLLDRVPKINVSKTEGQSWNDFKGKVEFKGCRFTYPSRPDVQVLRGLVVSVHPGQTLAFVGSSGCGKSTSVQLLERFYDPDEGQVLIDGRPSDSISVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSRTVSMEEIIDAAKKAYLHDFVMTLPDKYETQVGAQGSQLSRGQKQRIAIARAIVRNPKILLLDEATSALDTESEKTVQAALDEARQGRTCIVIAHRLSTIQSADIIAVMSQGEVIEKGTHDELMAKKAAYYKLV 1313 HSP 4 Score: 334.724 bits (857), Expect = 3.523e-93 Identity = 212/532 (39.85%), Postives = 311/532 (58.46%), Query Frame = 2
Query: 2462 ADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTN---VFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
A++ + + GI V + S Q + ++ + +R R F ++ +I WFD N++G L TR+SD+ +I + + I I + + F W+++LVV+A P++ + L + + + A ++G +A E + +IRTV+ EH + Y + + KK G G IF YA F G L+ ++T V + FG LI GAMNLG++ F G+ AA +F+ +DR P I+ FS +G LD + GDI+ +V F+YP RP + IL+D++ + + +T A VGPSG GK+T I L+QRFYD G + +D + I+ +NIQ LR L +V QEP LF+ +I ENI YG + T +II AAK +N ++FI++LP ++T VGE G SGGQKQRIAIARAL+RNPRILLLD ATSALD ESE +VQ+ALDKA++GRT + IAHRLSTI+NAD I+ F+ G+ E GTH +LL K G+Y +++LQ+
Sbjct: 129 AEMTNFALYYIGIGVGVLILSFFQITFWVSAAARQIQRIRKTYFRKIMCMEIGWFD--CNSVGELNTRMSDDINKINNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVIAVSPLLGLAAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEHKEAERYDRNLVQAQEWGIKKGMIIGVFQGYLWCIIFLCYALAFWF-GSKLVIETQELTPGGLVQVFFGVLI-GAMNLGQASPCLEAFASGRAAAKSIFDTIDREPEIDCFSDEGHTLD--KVKGDIEFHSVNFNYPSRPEVKILDDLNIVVKAGETTAFVGPSGSGKTTTIQLIQRFYDPSEGMVSLDGHDIRSLNIQWLRSLIGVVEQEPVLFATTIAENIRYGRAG--VTMQEIIEAAKQANAYNFIMSLPQTFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKARQGRTTISIAHRLSTIRNADVIVGFEHGRAVERGTHSQLLDKKGVYFTLVTLQNQ 652
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Xenopus
Match: ENSXETT00000003954.1 (bile salt export pump [Source:NCBI gene;Acc:100489744]) HSP 1 Score: 512.686 bits (1319), Expect = 0.000e+0 Identity = 260/575 (45.22%), Postives = 380/575 (66.09%), Query Frame = 2
Query: 2312 KVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMIS 4036
+V+ N E Y+ +G + A NG V P ++ILF +L + P++ +Q +I I IAVV+FV+ Q F SGE+LT+R R + F+AML Q+I WFD+ +N+ GALTTRL+ +A+Q++G +G +G V+ ++ ++GA++ I+F F W++SLVVL F+P++ + G+ Q +++ GF+N DK ALE +GK++ E I NIRTV+ L +E F++ Y++ ++ KK+H G +G Q IF AYAA+F G+L+ + VF A++ LG++ + P++ K KIAA F+LLDRVP + FS+ G E GDI+ N KF+YP RP ++L +S ++ S QT+A VG SGCGKST + LL+RFYD G +L+D + ++NI LR IVSQEP LF SI +NI YGD+++ +++I AAK +++H+F++ LPDQYET VG +GS S GQKQRIAIARA++R+P+ILLLDEATSALDTESEK VQ ALD+A++GRTC+ IAHRLSTIQ D I V G I E G+H L++ G Y ++++
Sbjct: 635 RVLKYNTKEWPYLLVGSIGAAINGVVTPLYAILFSQILGTFSLPDLNEQRREINGICILFVIIAVVSFVTQFFQGYAFAKSGERLTRRLRRVGFQAMLGQEIGWFDDNNNSPGALTTRLATDASQVQGATGSQIGMVVNSLTNIGASLIIAFYFSWKLSLVVLCFLPLLALAGVFQAKMLTGFANQDKNALEMAGKVSSEAIGNIRTVAGLGKETMFVEVYEKQLELPYKAAVKKSHVYGACFGFAQCVIFMAYAASFRFGGFLVNTDGIHYAVVFRVISAIVTSGTALGRASSFTPDYAKAKIAAEQFFKLLDRVPKTSVFSSSGD--KWEEFKGDIEFVNCKFTYPSRPVTMVLRGLSISVRSGQTLAFVGSSGCGKSTSVQLLERFYDPDEGKVLVDGHATANVNISFLRSKIGIVSQEPVLFEGSIADNIKYGDNSRDIPMEEVIEAAKKAHLHEFVMTLPDQYETNVGIQGSQLSRGQKQRIAIARAIVRDPKILLLDEATSALDTESEKTVQAALDEARKGRTCIAIAHRLSTIQTCDIIAVMSQGAIVEKGSHEALMALKGAYYKLVT 1207 HSP 2 Score: 463.381 bits (1191), Expect = 0.000e+0 Identity = 275/633 (43.44%), Postives = 387/633 (61.14%), Query Frame = 1
Query: 343 VMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRRERRDLC*C**RNYQRKSYKKTL 2241
VMM+ + ++ G PL++L++G T TY + TQ L N+ + T I + + +S + A I + F ++S+ +V+ + QI +R +FR +MR +IGWFD+ + G L ++ DDIN ++ I D++ ++ + FI G ++ FV WKLTLV +++SPLI + +++ + +LT + +AY KAG +A+EVL +IRT+ +F GEK E RY +NL+EA GIRK + G G + ++F Y+L FWYG+ L+ E G +L VFF L+A +GQA+P + +F R+AA I+E ID P ID M+EEG KL+ +G I F V F YP+R D L+ LS+SI+PG+T A VG SG GKSS +QL+QRFYD +G+V +D +++ LN WLR IG+V QEPVLF +I ENI YGR VT +I++A ANA FI LP K DTLVG+ G Q+SGGQKQRIAIARAL+RNPKILLLD ATSALD+ESE IVQ+AL+KV GRTTI +AHRLSTVR A +I G G+ E+G H ELM L G+Y LV Q +++ D+ + + R SY+ +L
Sbjct: 3 VMMVIGALCALIHGAAQPLMLLIYGMMTDTFITY----DRETQELQDINKECINDT-------IWWKNGTEYIVDNSTV-----------ASIDIKWFQGGFISL--WVVAAARQIQIVRKEYFRKIMRLDIGWFDSNSVGELNTRISDDINKINNAIADQVAIFIERISTFIFGFLIGFVGNWKLTLVIVAVSPLIGLGAGLMAVAVARLTGRELKAYAKAGSVADEVLSAIRTVAAFGGEKKEAERYDDNLEEAQTWGIRKGTIIGFFQGYMWCIIFLCYSLAFWYGSRLVIETRELSPGSLLQVFFGVLVAAMNLGQASPCLEAFASGRAAATIIYETIDLNPVIDCMSEEGHKLDQVKGDIEFHNVNFCYPSRPDIKSLSDLSISIKPGETTAFVGPSGSGKSSAVQLIQRFYDPTDGKVTLDGHDLRTLNTKWLRSLIGIVEQEPVLFATTIAENISYGRDGVTMNDIEKAAKDANAYNFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALIRNPKILLLDMATSALDNESEAIVQEALNKVQSGRTTISIAHRLSTVRTADIIVGFDGGRAVEKGNHEELMKLKGVYFTLVTLQNQNNSSEKTATEDVAAEKEKPFIRGSYRSSL 611 HSP 3 Score: 369.777 bits (948), Expect = 4.091e-105 Identity = 222/587 (37.82%), Postives = 335/587 (57.07%), Query Frame = 1
Query: 391 WPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
WP +++ S A + L L SQ I + S+PD+ E R ++G+ F IA+ F+ + F + + +R F+A++ Q IGWFD N+ GALT +L D + V G +IG ++ +I+AF WKL+LV L PL+ + + +K + + A AG ++ E +G+IRT+ E V Y + L+ K ++KS + G G ++F +YA F +G L+ +G + V + + + T +G+A+ + +A+ AA + F+++DRVP+ + GDK +F+G I F KFTYP+R +L GLS+S++ GQT+A VG+SGCGKS+ +QL++RFYD +EG+VL+D ++N+ +LR +IG+VSQEPVLF SI +NI+YG D+ EE+ +A A+ F+ LP++ +T VG +G+QLS GQKQRIAIARA+VR+PKILLLDEATSALD+ESEK VQ ALD+ +GRT I +AHRLST++ +I + G + E+G+H LMAL G Y LV
Sbjct: 644 WPYLLV-----GSIGAAINGVVTPLYAILFSQ---ILGTFSLPDLNEQRRE---------------INGICILFVIIAVVSFVTQFFQGYAFAKSGERLTRRLRRVGFQAMLGQEIGWFDDNNNSPGALTTRLATDASQVQGATGSQIGMVVNSLTNIGASLIIAFYFSWKLSLVVLCFLPLLALAGVFQAKMLTGFANQDKNALEMAGKVSSEAIGNIRTVAGLGKETMFVEVYEKQLELPYKAAVKKSHVYGACFGFAQCVIFMAYAASFRFGGFLVNT-DGIHYAVVFRVISAIVTSGTALGRASSFTPDYAKAKIAAEQFFKLLDRVPKTSVFSSSGDKWEEFKGDIEFVNCKFTYPSRPVTMVLRGLSISVRSGQTLAFVGSSGCGKSTSVQLLERFYDPDEGKVLVDGHATANVNISFLRSKIGIVSQEPVLFEGSIADNIKYGDNSRDIPMEEVIEAAKKAHLHEFVMTLPDQYETNVGIQGSQLSRGQKQRIAIARAIVRDPKILLLDEATSALDTESEKTVQAALDEARKGRTCIAIAHRLSTIQTCDIIAVMSQGAIVEKGSHEALMALKGAYYKLV 1206 HSP 4 Score: 309.301 bits (791), Expect = 2.521e-85 Identity = 211/585 (36.07%), Postives = 321/585 (54.87%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIE-QQSADIRTYVI-----------ALAGIAVVAFVSSVLQNVGFGN----SGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL--YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
+ +G + A+ +GA QP +++G + + E Q+ DI I + + VA + GF + + + + R F +++ DI WFD SN++G L TR+SD+ +I + + I I + I F W+++LV++A P+I +G L + + + A ++G +A E + IRTV+ E + Y + +++ +K GF G IF Y+ F L++ +S + + + FG L+ AMNLG++ F G+ AA ++E +D P I+ S +G LD + GDI+ NV F YP RP+I L+D+S +I +T A VGPSG GKS+ + L+QRFYD +G + +D + ++ +N + LR L IV QEP LF+ +I ENI+YG D I AAK +N ++FI++LP +++T VGE G SGGQKQRIAIARAL+RNP+ILLLD ATSALD ESE IVQ+AL+K Q GRT + IAHRLST++ AD I+ F G+ E G H EL+ G+Y +++LQ+
Sbjct: 5 MVIGALCALIHGAAQPLMLLIYGMMTDTFITYDRETQELQDINKECINDTIWWKNGTEYIVDNSTVASIDIKWFQGGFISLWVVAAARQIQIVRKEYFRKIMRLDIGWFD--SNSVGELNTRISDDINKINNAIADQVAIFIERISTFIFGFLIGFVGNWKLTLVIVAVSPLIGLGAGLMAVAVARLTGRELKAYAKAGSVADEVLSAIRTVAAFGGEKKEAERYDDNLEEAQTWGIRKGTIIGFFQGYMWCIIFLCYSLAFWYGSRLVIETRELSPGSLLQVFFGVLV-AAMNLGQASPCLEAFASGRAAATIIYETIDLNPVIDCMSEEGHKLD--QVKGDIEFHNVNFCYPSRPDIKSLSDLSISIKPGETTAFVGPSGSGKSSAVQLIQRFYDPTDGKVTLDGHDLRTLNTKWLRSLIGIVEQEPVLFATTIAENISYGRDGVTMND--IEKAAKDANAYNFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALIRNPKILLLDMATSALDNESEAIVQEALNKVQSGRTTISIAHRLSTVRTADIIVGFDGGRAVEKGNHEELMKLKGVYFTLVTLQNQ 582
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630]) HSP 1 Score: 577.4 bits (1487), Expect = 2.767e-175 Identity = 297/620 (47.90%), Postives = 411/620 (66.29%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRP 2160
GV +F Y++ SD ++M+ I SI G PL+++VFG+ T N +E ++ +S N+ M+ + ++G+ + + +YV ++F+ +++ QI IR+ FF AV+RQ IGWFD ++G L +L DD++ ++ GIGDKI ++Q F+ G I+ FV+GWKLTLV L+ISP++ + +K + T K +AY KAG +AEEVL SIRT+ +F G+ E+ RY +NL+EA +IGI+K+I +G +++ +YAL FWYG +LI G G+ +G +L VFF+ ++ +GQ +P++ +F AR AA IF IID P+ID ++EG K + +G I FK VKFTYP+RTD +L GL+L+I G+TVALVG+SGCGKS+ +QL+QRFYD EG + +D +I+ LN+ +LRE IGVVSQEP+LF +I +NIRYGR DVT EEI++A ANA FI LP+K +TLVG+RGTQLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI+VAHRLST+RNA VI G +G + EQG H ELM G+Y +LV Q +P
Sbjct: 49 GVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGNF-------------------SLEALIN--------ASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIING-GYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQVGKP 640 HSP 2 Score: 505.368 bits (1300), Expect = 2.415e-150 Identity = 269/582 (46.22%), Postives = 385/582 (66.15%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVS---KKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F KVM LN E Y +G + A+ NGA QP+F+I+F ++ A P E + A Y + + ++F++ LQ V FG+ L ++I WFD+ N+ GALTTRL+ +A+Q++G +G L + I ++G AV ISF + W+++L++LA VPII ++Q+++ G + DK LE++GKI+ + + NIRTV +LTRE F Y+ K L+G KKAH G Y L+QA ++ AYA F YL+ + +VF+ F A++FGAM LG++ + AP++ K I+AAH+F LL+RVP I+S+S +G +N G+++ + V+F+YP RP+I +L + ++ +T+ALVG SGCGKST + LL+RFYD G +L+D + ++D+NIQ +R IVSQEP LF SI ENIAYGD+++ T +I AAK +NIH FI L D+Y T+VG+KG+ SGGQKQRIAIARAL+R P+ILLLDEATSALDTESEK+VQ+ALDKA+ GRTC+VIAHRLSTIQNADKI V ++GK+ E GTH +LL G+Y ++++Q
Sbjct: 697 FLKVMRLNKTEWPYFVVGIICAMINGATQPAFAIIFSKIIGVFAGPASEMR-AKSTMYSLLFLALGGISFITFFLQ-VRFGHVYFTL--------------KEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYE---KNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGE--KPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1257 HSP 3 Score: 402.519 bits (1033), Expect = 1.117e-115 Identity = 219/479 (45.72%), Postives = 314/479 (65.55%), Query Frame = 1
Query: 730 QNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLV--GDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+ IGWFD ++GALT +L D + V G ++ + Q A +I++F+ GW+LTL+ L+I P+I L+ K +K + KAG I+ + + +IRT+VS E+ A Y +NL+ + I+K+ L G L +M+ +YA F +GA L+ E L+ D+ +VF + + +GQ + + +A +AA IF +++RVP ID ++EG+K + G + F+ V+F YPTR D +L GL +S++ G+T+ALVG+SGCGKS+ + L++RFYD GEVL+D +++DLN+ W+R Q+G+VSQEP+LF CSI ENI YG +VT EI+ A AN +FI+ L +K +T VGD+GTQ+SGGQKQRIAIARALVR PKILLLDEATSALD+ESEK+VQ+ALDK GRT I++AHRLST++NA I I++GKV EQGTH +L+ L G+Y LV Q
Sbjct: 782 KEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAE---LLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGEKPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1257 HSP 4 Score: 385.185 bits (988), Expect = 5.855e-110 Identity = 230/589 (39.05%), Postives = 349/589 (59.25%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG--------------FLLPAMANP--NIEQQSADIRTYVIALA-GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 4051
++ + G +++I +GA P I+FG F L A+ N N+++Q Y L G+ + A+V + ++G+Q+ KR RS F A+L+Q+I WFD N+ G L TRL+D+ ++I G+ + ++ ++ + I F W+++LV+LA P++ + +++ F+N + A ++G +A E + +IRTV + I+ Y++ +++ KKA G I+ AYA F L++ + +V F A+I GA +G++ F + AA +F ++D P I+SFS +G L + + GDI+ +NVKF+YP R +I +L ++ NI S +T+ALVG SGCGKST + L+QRFYD G I +D I+ +NI+ LR++ +VSQEP LF +I +NI YG T ++I A K +N ++FI+ LPD++ET VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +V+AHRLSTI+NAD I F +G I E G H EL+ K G+Y +++LQ K
Sbjct: 61 DRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGNFSLEALINASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTL---SAGKQI-KRIRSNFFHAVLRQEIGWFD--VNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEG--LKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGRED--VTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQVGK 639
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630]) HSP 1 Score: 574.704 bits (1480), Expect = 3.893e-174 Identity = 296/616 (48.05%), Postives = 409/616 (66.40%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
GV +F Y++ SD ++M+ I SI G PL+++VFG+ T N +E ++ +S N+ M+ + ++G+ + + +YV ++F+ +++ QI IR+ FF AV+RQ IGWFD ++G L +L DD++ ++ GIGDKI ++Q F+ G I+ FV+GWKLTLV L+ISP++ + +K + T K +AY KAG +AEEVL SIRT+ +F G+ E+ RY +NL+EA +IGI+K+I +G +++ +YAL FWYG +LI G G+ +G +L VFF+ ++ +GQ +P++ +F AR AA IF IID P+ID ++EG K + +G I FK VKFTYP+RTD +L GL+L+I G+TVALVG+SGCGKS+ +QL+QRFYD EG + +D +I+ LN+ +LRE IGVVSQEP+LF +I +NIRYGR DVT EEI++A ANA FI LP+K +TLVG+RGTQLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI+VAHRLST+RNA VI G +G + EQG H ELM G+Y +LV Q
Sbjct: 49 GVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGNF-------------------SLEALIN--------ASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIING-GYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQ 636 HSP 2 Score: 541.576 bits (1394), Expect = 1.230e-162 Identity = 278/582 (47.77%), Postives = 397/582 (68.21%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVS---KKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F KVM LN E Y +G + A+ NGA QP+F+I+F ++ A P E + A Y + + ++F++ LQ FG +GE LTKR R +F++ML+Q+I WFD+ N+ GALTTRL+ +A+Q++G +G L + I ++G AV ISF + W+++L++LA VPII ++Q+++ G + DK LE++GKI+ + + NIRTV +LTRE F Y+ K L+G KKAH G Y L+QA ++ AYA F YL+ + +VF+ F A++FGAM LG++ + AP++ K I+AAH+F LL+RVP I+S+S +G +N G+++ + V+F+YP RP+I +L + ++ +T+ALVG SGCGKST + LL+RFYD G +L+D + ++D+NIQ +R IVSQEP LF SI ENIAYGD+++ T +I AAK +NIH FI L D+Y T+VG+KG+ SGGQKQRIAIARAL+R P+ILLLDEATSALDTESEK+VQ+ALDKA+ GRTC+VIAHRLSTIQNADKI V ++GK+ E GTH +LL G+Y ++++Q
Sbjct: 695 FLKVMRLNKTEWPYFVVGIICAMINGATQPAFAIIFSKIIGVFAGPASEMR-AKSTMYSLLFLALGGISFITFFLQGFTFGKAGEILTKRLRLWSFKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYE---KNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGE--KPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1270 HSP 3 Score: 411.764 bits (1057), Expect = 1.323e-118 Identity = 222/485 (45.77%), Postives = 320/485 (65.98%), Query Frame = 1
Query: 712 FRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLV--GDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
F++++RQ IGWFD ++GALT +L D + V G ++ + Q A +I++F+ GW+LTL+ L+I P+I L+ K +K + KAG I+ + + +IRT+VS E+ A Y +NL+ + I+K+ L G L +M+ +YA F +GA L+ E L+ D+ +VF + + +GQ + + +A +AA IF +++RVP ID ++EG+K + G + F+ V+F YPTR D +L GL +S++ G+T+ALVG+SGCGKS+ + L++RFYD GEVL+D +++DLN+ W+R Q+G+VSQEP+LF CSI ENI YG +VT EI+ A AN +FI+ L +K +T VGD+GTQ+SGGQKQRIAIARALVR PKILLLDEATSALD+ESEK+VQ+ALDK GRT I++AHRLST++NA I I++GKV EQGTH +L+ L G+Y LV Q
Sbjct: 789 FKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAE---LLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGEKPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1270 HSP 4 Score: 385.185 bits (988), Expect = 7.158e-110 Identity = 229/587 (39.01%), Postives = 349/587 (59.45%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG--------------FLLPAMANP--NIEQQSADIRTYVIALA-GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
++ + G +++I +GA P I+FG F L A+ N N+++Q Y L G+ + A+V + ++G+Q+ KR RS F A+L+Q+I WFD N+ G L TRL+D+ ++I G+ + ++ ++ + I F W+++LV+LA P++ + +++ F+N + A ++G +A E + +IRTV + I+ Y++ +++ KKA G I+ AYA F L++ + +V F A+I GA +G++ F + AA +F ++D P I+SFS +G L + + GDI+ +NVKF+YP R +I +L ++ NI S +T+ALVG SGCGKST + L+QRFYD G I +D I+ +NI+ LR++ +VSQEP LF +I +NI YG T ++I A K +N ++FI+ LPD++ET VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +V+AHRLSTI+NAD I F +G I E G H EL+ K G+Y +++LQ+
Sbjct: 61 DRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGNFSLEALINASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTL---SAGKQI-KRIRSNFFHAVLRQEIGWFD--VNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEG--LKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGRED--VTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQT 637
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630]) HSP 1 Score: 574.318 bits (1479), Expect = 6.048e-174 Identity = 296/622 (47.59%), Postives = 411/622 (66.08%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTS------QMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
GV +F Y++ SD ++M+ I SI G PL+++VFG+ T Q+ + + + +L +S N+ M+ + ++G+ + + +YV ++F+ +++ QI IR+ FF AV+RQ IGWFD ++G L +L DD++ ++ GIGDKI ++Q F+ G I+ FV+GWKLTLV L+ISP++ + +K + T K +AY KAG +AEEVL SIRT+ +F G+ E+ RY +NL+EA +IGI+K+I +G +++ +YAL FWYG +LI G G+ +G +L VFF+ ++ +GQ +P++ +F AR AA IF IID P+ID ++EG K + +G I FK VKFTYP+RTD +L GL+L+I G+TVALVG+SGCGKS+ +QL+QRFYD EG + +D +I+ LN+ +LRE IGVVSQEP+LF +I +NIRYGR DVT EEI++A ANA FI LP+K +TLVG+RGTQLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI+VAHRLST+RNA VI G +G + EQG H ELM G+Y +LV Q
Sbjct: 49 GVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGSGNFSLEALIN-------------------------------ASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIING-GYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQ 638 HSP 2 Score: 541.962 bits (1395), Expect = 1.287e-162 Identity = 278/582 (47.77%), Postives = 397/582 (68.21%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVS---KKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F KVM LN E Y +G + A+ NGA QP+F+I+F ++ A P E + A Y + + ++F++ LQ FG +GE LTKR R +F++ML+Q+I WFD+ N+ GALTTRL+ +A+Q++G +G L + I ++G AV ISF + W+++L++LA VPII ++Q+++ G + DK LE++GKI+ + + NIRTV +LTRE F Y+ K L+G KKAH G Y L+QA ++ AYA F YL+ + +VF+ F A++FGAM LG++ + AP++ K I+AAH+F LL+RVP I+S+S +G +N G+++ + V+F+YP RP+I +L + ++ +T+ALVG SGCGKST + LL+RFYD G +L+D + ++D+NIQ +R IVSQEP LF SI ENIAYGD+++ T +I AAK +NIH FI L D+Y T+VG+KG+ SGGQKQRIAIARAL+R P+ILLLDEATSALDTESEK+VQ+ALDKA+ GRTC+VIAHRLSTIQNADKI V ++GK+ E GTH +LL G+Y ++++Q
Sbjct: 705 FLKVMRLNKTEWPYFVVGIICAMINGATQPAFAIIFSKIIGVFAGPASEMR-AKSTMYSLLFLALGGISFITFFLQGFTFGKAGEILTKRLRLWSFKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYE---KNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGE--KPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1280 HSP 3 Score: 411.764 bits (1057), Expect = 1.321e-118 Identity = 222/485 (45.77%), Postives = 320/485 (65.98%), Query Frame = 1
Query: 712 FRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLV--GDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
F++++RQ IGWFD ++GALT +L D + V G ++ + Q A +I++F+ GW+LTL+ L+I P+I L+ K +K + KAG I+ + + +IRT+VS E+ A Y +NL+ + I+K+ L G L +M+ +YA F +GA L+ E L+ D+ +VF + + +GQ + + +A +AA IF +++RVP ID ++EG+K + G + F+ V+F YPTR D +L GL +S++ G+T+ALVG+SGCGKS+ + L++RFYD GEVL+D +++DLN+ W+R Q+G+VSQEP+LF CSI ENI YG +VT EI+ A AN +FI+ L +K +T VGD+GTQ+SGGQKQRIAIARALVR PKILLLDEATSALD+ESEK+VQ+ALDK GRT I++AHRLST++NA I I++GKV EQGTH +L+ L G+Y LV Q
Sbjct: 799 FKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAE---LLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGEKPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1280 HSP 4 Score: 384.8 bits (987), Expect = 1.236e-109 Identity = 229/589 (38.88%), Postives = 349/589 (59.25%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG----------------FLLPAMANP--NIEQQSADIRTYVIALA-GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
++ + G +++I +GA P I+FG F L A+ N N+++Q Y L G+ + A+V + ++G+Q+ KR RS F A+L+Q+I WFD N+ G L TRL+D+ ++I G+ + ++ ++ + I F W+++LV+LA P++ + +++ F+N + A ++G +A E + +IRTV + I+ Y++ +++ KKA G I+ AYA F L++ + +V F A+I GA +G++ F + AA +F ++D P I+SFS +G L + + GDI+ +NVKF+YP R +I +L ++ NI S +T+ALVG SGCGKST + L+QRFYD G I +D I+ +NI+ LR++ +VSQEP LF +I +NI YG T ++I A K +N ++FI+ LPD++ET VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +V+AHRLSTI+NAD I F +G I E G H EL+ K G+Y +++LQ+
Sbjct: 61 DRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGSGNFSLEALINASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTL---SAGKQI-KRIRSNFFHAVLRQEIGWFD--VNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEG--LKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGRED--VTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQT 639
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630]) HSP 1 Score: 572.778 bits (1475), Expect = 1.038e-173 Identity = 295/616 (47.89%), Postives = 409/616 (66.40%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
GV +F Y++ SD ++M+ I SI G PL+++VFG+ +T + + ST+ + R K+ + + ++G+ + + +YV ++F+ +++ QI IR+ FF AV+RQ IGWFD ++G L +L DD++ ++ GIGDKI ++Q F+ G I+ FV+GWKLTLV L+ISP++ + +K + T K +AY KAG +AEEVL SIRT+ +F G+ E+ RY +NL+EA +IGI+K+I +G +++ +YAL FWYG +LI G G+ +G +L VFF+ ++ +GQ +P++ +F AR AA IF IID P+ID ++EG K + +G I FK VKFTYP+RTD +L GL+L+I G+TVALVG+SGCGKS+ +QL+QRFYD EG + +D +I+ LN+ +LRE IGVVSQEP+LF +I +NIRYGR DVT EEI++A ANA FI LP+K +TLVG+RGTQLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI+VAHRLST+RNA VI G +G + EQG H ELM G+Y +LV Q
Sbjct: 49 GVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGD--------------MTDSFVNVGQVAFHSTAHARANDTGRRKRCRY--------------AYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIING-GYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQ 635 HSP 2 Score: 520.39 bits (1339), Expect = 1.612e-155 Identity = 272/582 (46.74%), Postives = 385/582 (66.15%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVS---KKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F KVM LN E Y +G + A+ NGA QP+F+I+F ++ A P E + A Y + + ++F++ LQ FG +GE LTKR R +F++ML+Q+I WFD+ N+ GALTTRL+ +A+Q++G +G L + I ++G AV ISF + W+++L++LA VPII ++Q+++ G + DK LE++GKI+ + + NIRTV +LTRE F Y+ K L+G KKAH G Y L+QA ++ AYA F YL+ + +VF+ F A++FGAM LG++ + AP++ K I+AAH+F LL+R + G I + V+F+YP RP+I +L + ++ +T+ALVG SGCGKST + LL+RFYD G +L+D + ++D+NIQ +R IVSQEP LF SI ENIAYGD+++ T +I AAK +NIH FI L D+Y T+VG+KG+ SGGQKQRIAIARAL+R P+ILLLDEATSALDTESEK+VQ+ALDKA+ GRTC+VIAHRLSTIQNADKI V ++GK+ E GTH +LL G+Y ++++Q
Sbjct: 694 FLKVMRLNKTEWPYFVVGIICAMINGATQPAFAIIFSKIIGVFAGPASEMR-AKSTMYSLLFLALGGISFITFFLQGFTFGKAGEILTKRLRLWSFKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYE---KNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLER------------------LGGYITFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1253 HSP 3 Score: 390.578 bits (1002), Expect = 9.226e-112 Identity = 217/485 (44.74%), Postives = 311/485 (64.12%), Query Frame = 1
Query: 712 FRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLV--GDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
F++++RQ IGWFD ++GALT +L D + V G ++ + Q A +I++F+ GW+LTL+ L+I P+I L+ K +K + KAG I+ + + +IRT+VS E+ A Y +NL+ + I+K+ L G L +M+ +YA F +GA L+ E L+ D+ +VF + + +GQ + + +A +AA IF +++R+ G ITF+ V+F YPTR D +L GL +S++ G+T+ALVG+SGCGKS+ + L++RFYD GEVL+D +++DLN+ W+R Q+G+VSQEP+LF CSI ENI YG +VT EI+ A AN +FI+ L +K +T VGD+GTQ+SGGQKQRIAIARALVR PKILLLDEATSALD+ESEK+VQ+ALDK GRT I++AHRLST++NA I I++GKV EQGTH +L+ L G+Y LV Q
Sbjct: 788 FKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAE---LLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERLG----------------GYITFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDKARTGRTCIVIAHRLSTIQNADKIAVIQNGKVVEQGTHQQLLQLKGVYYTLVTIQ 1253 HSP 4 Score: 379.407 bits (973), Expect = 4.970e-108 Identity = 226/586 (38.57%), Postives = 345/586 (58.87%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFLLP---------------AMANPNIEQQSADIRTYVIALA-GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
++ + G +++I +GA P I+FG + A AN ++ Y L G+ + A+V + ++G+Q+ KR RS F A+L+Q+I WFD N+ G L TRL+D+ ++I G+ + ++ ++ + I F W+++LV+LA P++ + +++ F+N + A ++G +A E + +IRTV + I+ Y++ +++ KKA G I+ AYA F L++ + +V F A+I GA +G++ F + AA +F ++D P I+SFS +G L + + GDI+ +NVKF+YP R +I +L ++ NI S +T+ALVG SGCGKST + L+QRFYD G I +D I+ +NI+ LR++ +VSQEP LF +I +NI YG T ++I A K +N ++FI+ LPD++ET VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +V+AHRLSTI+NAD I F +G I E G H EL+ K G+Y +++LQ+
Sbjct: 61 DRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVAFHSTAHARANDTGRRKRCRYAYYYSGLGFGVLLFAYVQISFWTL---SAGKQI-KRIRSNFFHAVLRQEIGWFD--VNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEG--LKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGRED--VTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDKAREGRTTIVVAHRLSTIRNADVIAGFDNGVIVEQGGHKELMDKGGVYFNLVTLQT 636
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Mouse
Match: Abcb1a (ATP-binding cassette, sub-family B (MDR/TAP), member 1A [Source:MGI Symbol;Acc:MGI:97570]) HSP 1 Score: 563.148 bits (1450), Expect = 2.755e-170 Identity = 297/612 (48.53%), Postives = 400/612 (65.36%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+F YA D + M+ + +I GV PL++L+FG+ T A+ + ++++ T+ +E +EE + Y +T GI + I++Y+ V+F+ + + QI IR FF A+M Q IGWFD + G L +L DD++ ++ GIGDKIG Q A F GG I+ F RGWKLTLV L+ISP++ + + +K + T K AY KAG +AEEVL +IRT+++F G+K E+ RY NL+EA ++GI+K+I +G L++ SYAL FWYG SL+ + +G +L VFFS L+ +GQA+P++ +F AR AA E+F+IID P ID ++ G K ++ QG + FK + F+YP+R + IL GL+L ++ GQTVALVG SGCGKS+ +QLMQR YD +G V ID +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR DVT +EI++A+ ANA FI LP++ DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI++AHRLSTVRNA VI G G + EQG H ELM G+Y LV Q
Sbjct: 38 MFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLIFGDMTDSFAS----VGNVSKNSTNMSEA-DKRAMFAKLEEEMTTYAYYYT------------------GIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKE-YSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQ 625 HSP 2 Score: 556.984 bits (1434), Expect = 3.795e-168 Identity = 273/585 (46.67%), Postives = 408/585 (69.74%), Query Frame = 2
Query: 2300 AGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMAN---PNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
A F++++ LN+ E Y +G AI NG +QP+FS++F ++ N P ++Q++++ + + + GI ++F++ LQ FG +GE LTKR R + F++ML+QD++WFD+P N GALTTRL+++A Q++G +G L + I ++G + IS + WQ++L++LA VPII + G+++++++ G + DK LE SGKIA E IEN RTV +LTRE F Y ++++ KKAH G + TQA ++ +YAA F YL+ + NV + F A++FGAM +G+ + AP++ K ++A+H+ ++++ P I+S+ST G L + G+++ V F+YP RP+I +L +S + QT+ALVG SGCGKST++ LL+RFYD + G++ +D +IK +N+Q LR IVSQEP LF SI ENIAYGD++++ + ++I+ AAK +NIH FI +LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+V ++GK+ E GTH +LL++ GIY M+S+Q+
Sbjct: 691 ASFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSVIFSKVVGVFTNGGPPETQRQNSNLFSLLFLILGI--ISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVTQQLMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQG--LKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQA 1271 HSP 3 Score: 409.068 bits (1050), Expect = 7.222e-118 Identity = 228/532 (42.86%), Postives = 332/532 (62.41%), Query Frame = 1
Query: 568 SSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+SN+F+L+ F + + FI ++ F + +R F++++RQ++ WFD +GALT +L +D V G ++ + Q A GII++ + GW+LTL+ L+I P+I I ++ K + K + +G IA E + + RT+VS R +K+E Y ++L + ++K+ + G +M+ SYA F +GA L+ + ++L+VF + + +GQ + + +A +A+ I II++ PEID + +G K N +G + F V F YPTR P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G V +D IK LNV WLR Q+G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI++LP+K +T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I I++GKV+E GTH +L+A G+Y +V Q
Sbjct: 747 NSNLFSLL------FLILGIISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETM-YAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVTQ-QLMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1270 HSP 4 Score: 365.54 bits (937), Expect = 1.777e-103 Identity = 223/589 (37.86%), Postives = 341/589 (57.89%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMAN-PNIEQQS-----ADIR-----------TYVIALAGIAVVAFVSSVLQNVGFG--NSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
++ Y+ +G ++AI +G P ++FG + + A+ N+ + S AD R TY GI + + +Q V F +G Q+ K R F A++ Q+I WFD +++G L TRL+D+ ++I G+ +G A+ + I F W+++LV+LA P++ + + +I+ F++ + A ++G +A E + IRTV + ++ Y +++ KKA G I+ +YA F L++ + V F +++ GA ++G++ F + AA +F+++D P I+SFS G D N+ G+++ +N+ FSYP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD L+G + ID I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP Q++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +VIAHRLST++NAD I F G I E G H EL+ + GIY +++ Q+
Sbjct: 46 DRLYMLVGTLAAIIHGVALPLMMLIFGDMTDSFASVGNVSKNSTNMSEADKRAMFAKLEEEMTTYAYYYTGIGAGVLIVAYIQ-VSFWCLAAGRQIHK-IRQKFFHAIMNQEIGWFD--VHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPD--NIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRED--VTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQT 626
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Mouse
Match: Abcb1b (ATP-binding cassette, sub-family B (MDR/TAP), member 1B [Source:MGI Symbol;Acc:MGI:97568]) HSP 1 Score: 563.148 bits (1450), Expect = 3.311e-170 Identity = 303/616 (49.19%), Postives = 404/616 (65.58%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
GV +F YA D + MI + +I G PL++LVFGN T + S+ +T+Q+ P +ST I +S+S++ M+ + + GI + I++Y+ V+ + + + QI IR FF A+M Q IGWFD + G L +L DD++ ++ GIGDKIG Q F+ G I+ F+ GWKLTLV L++SPLI + + +K + T K +AY KAG +AEEVL +IRT+++F G++ E+ RY +NL+EA +GI+K+I +G+ L++ SYAL FWYG SL+ N + +G++L VFFS L+ T IG P++ +F AR AA EIF+IID P ID + +G K + G + FK V F YP+R++ IL GL+L ++ GQTVALVG SGCGKS+ +QLMQR YD EG V ID +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR DVT +EI++A+ ANA FI LP++ DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI++AHRLSTVRNA VI G G + EQG H ELM G+Y LV Q
Sbjct: 34 GVFGMFRYADWLDKLCMILGTLAAIIHGTLLPLLMLVFGNMTDSFTKAE---ASILPSITNQSGP--NSTLI---------------ISNSSLEEEMAIYAYYYTGIGAGVLIVAYIQVSLWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINDGIGDKIGMFFQSITTFLAGFIIGFISGWKLTLVILAVSPLIGLSSALWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQQKELERYNKNLEEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLS-NEYSIGEVLTVFFSILLGTFSIGHLAPNIEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPDSIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQ 628 HSP 2 Score: 540.421 bits (1391), Expect = 2.396e-162 Identity = 263/577 (45.58%), Postives = 397/577 (68.80%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA-NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMI 4033
F++++ LN E Y+ +G + A+ NG +QP F+I+F ++ + + + E + + + + + +++FV+ Q FG +GE LTKR R + F++ML+QDI+WFD+ N+ G+LTTRL+ +A+ ++G G L V + ++G V +S + WQ++L+++ +P+I +GGI++++++ G + DK LE SGKIA E IEN RT+ +LTRE F Y ++++ KKAH G + TQA ++ +YAA F YL+ + NV + F A++FGAM G + + AP++ K K++A+H+ ++++ P I+S+ST+G L + G++K V+F+YP RPNI +L +S + QT+ALVG SGCGKST++ LL+RFYD + G++ +D +IK +N+Q LR IVSQEP LF SI ENIAYGD+++ + ++I+ AAK +NIH FI +LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+V ++GK+ E GTH +LL++ GIY M+
Sbjct: 695 FWRILNLNLSEWPYLLVGVLCAVINGCIQPVFAIVFSRIVGVFSRDDDHETKRQNCNLFSLFFLVMGLISFVTYFFQGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKGAMGARLAVVTQNVANLGTGVILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKQLEISGKIATEAIENFRTIVSLTREQKFETMYAQSLQVPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAQQLMTFENVMLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEG--LKPTLLEGNVKFNGVQFNYPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMV 1269 HSP 3 Score: 405.601 bits (1041), Expect = 1.105e-116 Identity = 226/525 (43.05%), Postives = 331/525 (63.05%), Query Frame = 1
Query: 610 FAGIALAMFIMSYVSVTF--FVITSQHQI--SAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNR 2157
F+ L M ++S+V+ F F +I +R F++++RQ+I WFD ++G+LT +L D ++V +G ++ + Q A G+IL+ V GW+LTL+ + I PLI++ ++ K + K + +G IA E + + RTIVS R +K+E Y ++L + ++K+ + G +M+ SYA F +GA L+ + ++++VF + + G + + +A+ +A+ I II++ PEID + EG K +G + F V+F YPTR + P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G V +D IK LNV WLR +G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI++LP+K +T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I I++GKV+E GTH +L+A G+Y +V R
Sbjct: 753 FSLFFLVMGLISFVTYFFQGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKGAMGARLAVVTQNVANLGTGVILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKQLEISGKIATEAIENFRTIVSLTREQKFETM-YAQSLQVPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAQ-QLMTFENVMLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEGLKPTLLEGNVKFNGVQFNYPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMVQAGAKR 1275 HSP 4 Score: 376.711 bits (966), Expect = 4.036e-107 Identity = 230/598 (38.46%), Postives = 350/598 (58.53%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFL-----------LPAMAN---PN---------IEQQSADIRTYVIAL-AGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPN---FGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
+K + LG ++AI +G + P ++FG + LP++ N PN +E++ A Y + AG+ +VA++ L + +G Q+ K R F A++ Q+I WFD +++G L TRL+D+ ++I G+ +G +I + A I F W+++LV+LA P+I + L +++ F+N + A ++G +A E + IRTV + ++ Y + +++ KKA G+ ++ +YA F L+L N + V F +++ G ++G +APN F + AA +F+++D P I+SFST G D ++ G+++ +NV F+YP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD L G + ID I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP Q++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +VIAHRLST++NAD I F G I E G H EL+ + GIY +++ Q+
Sbjct: 46 DKLCMILGTLAAIIHGTLLPLLMLVFGNMTDSFTKAEASILPSITNQSGPNSTLIISNSSLEEEMAIYAYYYTGIGAGVLIVAYIQVSLWCLA---AGRQIHK-IRQKFFHAIMNQEIGWFD--VHDVGELNTRLTDDVSKINDGIGDKIGMFFQSITTFLAGFIIGFISGWKLTLVILAVSPLIGLSSALWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQQKELERYNKNLEEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLSNEYSIGEVLTVFFSILLGTFSIGH---LAPNIEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPD--SIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRED--VTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTR 630
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Mouse
Match: Abcb4 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 [Source:MGI Symbol;Acc:MGI:97569]) HSP 1 Score: 533.872 bits (1374), Expect = 1.647e-164 Identity = 286/616 (46.43%), Postives = 403/616 (65.42%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G+ LF Y+ D + M +M+I G PL+++VFG T + ++++ F + P+ S S+ + I+ + M+ + ++G+ + + +Y+ V+F+ + + QI IR FF A++RQ +GWFD + + L +L DD++ + GIGDK+G Q A F G I+ F+RGWKLTLV ++ISP++ + + +K + + K AY KAG +AEE LG+IRT+++F G+ E+ RY ++L+ A KIGI+K+I +G+ L++ SYAL FWYG++L+ + +G+ + VFFS L+ +GQA P + +F AR AA IF+IID P+ID +E G K ++ +G + F V F+YP+R + IL GL+L ++ GQTVALVG SGCGKS+ +QL+QR YD EG++ ID +I++ NV LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP K DTLVGDRG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE VQ ALDK EGRTTI++AHRLST+RNA VI G +DG + EQG+H+ELM G+Y LV+ Q
Sbjct: 38 GLLTLFRYSDWQDKLFMFLGTLMAIAHGSGLPLMMIVFGEMTDK------FVDNTGNF----SLPVNFSLSMLNPGRILEEE--------------MTRYAYYYSGLGGGVLVAAYIQVSFWTLAAGRQIKKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKE-YTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFRLVNMQ 628 HSP 2 Score: 366.696 bits (940), Expect = 5.399e-107 Identity = 230/590 (38.98%), Postives = 346/590 (58.64%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG------------FLLP-----AMANPN--IEQQSADIRTYVIALAG-IAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+K ++FLG + AI +G+ P I+FG F LP +M NP +E++ Y L G + V A++ + +G Q+ K+ R F A+L+Q++ WFD L TRL+D+ ++I G+ +G AI + A + F W+++LV++A PI+ + + +I+ FS+ + A ++G +A E + IRTV ++ ++ Y++ ++ KKA + G+ I+ +YA F L++ + N F +++ GA ++G++ F + AA +F+++D P I+SFS G D N+ G+++ +V FSYP R NI IL ++ + S QT+ALVG SGCGKST + LLQR YD G I ID I++ N++ LR++ +VSQEP LFS +I ENI YG T D+I A K +N +DFI+ LP +++T VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE VQ ALDKA+EGRT +VIAHRLSTI+NAD I F+DG I E G+H EL+ K GIY +++++Q+
Sbjct: 50 DKLFMFLGTLMAIAHGSGLPLMMIVFGEMTDKFVDNTGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLVAAYIQVSFWTLA---AGRQI-KKIRQKFFHAILRQEMGWFDIKGTT--ELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPD--NIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGN--VTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFRLVNMQT 629 HSP 3 Score: 149.443 bits (376), Expect = 1.580e-35 Identity = 74/192 (38.54%), Postives = 123/192 (64.06%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPN---IEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSD 2872
F KV+ LN E Y +G V AI NGA+QP+FSI+ ++ A+ P ++QQ ++ + + G+ V++F + LQ FG +GE LT R RS+ F+AML+QD++WFD+ N+ GAL+TRL+ +A Q++G +G L + ++G + ISF + WQ++L++L+ VP I V GI++++++ G + D
Sbjct: 694 FLKVLKLNKTEWPYFVVGTVCAIANGALQPAFSIILSEMI-AIFGPGDDAVKQQKCNM--FSLVFLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRD 882 HSP 4 Score: 66.2402 bits (160), Expect = 6.321e-10 Identity = 37/125 (29.60%), Postives = 67/125 (53.60%), Query Frame = 1
Query: 574 NIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIIC 942
N+F+L+ F G+ + F ++ F + + +R+ F+A++RQ++ WFD ++GAL+ +L D V G ++ + Q A GII++F+ GW+LTL+ LS+ P I +
Sbjct: 749 NMFSLV------FLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVA 867
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Mouse
Match: Abcb4 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 [Source:MGI Symbol;Acc:MGI:97569]) HSP 1 Score: 536.569 bits (1381), Expect = 5.928e-161 Identity = 271/583 (46.48%), Postives = 398/583 (68.27%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPN---IEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F KV+ LN E Y +G V AI NGA+QP+FSI+ ++ A+ P ++QQ ++ + + G+ V++F + LQ FG +GE LT R RS+ F+AML+QD++WFD+ N+ GAL+TRL+ +A Q++G +G L + ++G + ISF + WQ++L++L+ VP I V GI++++++ G + DK +E +GKIA E IENIRTV +LT+E F Y E + +KAH G + ++QA ++ +YA F YL++ + +V + F A++ GA+ LG + + AP++ K K++AA++F L +R P I+S+S +G+ D G + V F+YP R N+ +L +S + QT+ALVG SGCGKST++ LL+RFYD + G++L+D + K +N+Q LR IVSQEP LF SI ENIAYGD++++ D+I+ AAK +NIH FI LP +Y T+VG+KG+ SGGQKQRIAIARAL+R PR+LLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+V ++GK+ E GTH +LL++ GIY M+++Q+
Sbjct: 694 FLKVLKLNKTEWPYFVVGTVCAIANGALQPAFSIILSEMI-AIFGPGDDAVKQQKCNM--FSLVFLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSGEGLWPD--KFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVPHDEIVRAAKEANIHPFIETLPQKYNTRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMVNIQA 1271 HSP 2 Score: 533.872 bits (1374), Expect = 3.939e-160 Identity = 286/616 (46.43%), Postives = 403/616 (65.42%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G+ LF Y+ D + M +M+I G PL+++VFG T + ++++ F + P+ S S+ + I+ + M+ + ++G+ + + +Y+ V+F+ + + QI IR FF A++RQ +GWFD + + L +L DD++ + GIGDK+G Q A F G I+ F+RGWKLTLV ++ISP++ + + +K + + K AY KAG +AEE LG+IRT+++F G+ E+ RY ++L+ A KIGI+K+I +G+ L++ SYAL FWYG++L+ + +G+ + VFFS L+ +GQA P + +F AR AA IF+IID P+ID +E G K ++ +G + F V F+YP+R + IL GL+L ++ GQTVALVG SGCGKS+ +QL+QR YD EG++ ID +I++ NV LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP K DTLVGDRG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE VQ ALDK EGRTTI++AHRLST+RNA VI G +DG + EQG+H+ELM G+Y LV+ Q
Sbjct: 38 GLLTLFRYSDWQDKLFMFLGTLMAIAHGSGLPLMMIVFGEMTDK------FVDNTGNF----SLPVNFSLSMLNPGRILEEE--------------MTRYAYYYSGLGGGVLVAAYIQVSFWTLAAGRQIKKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKE-YTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFRLVNMQ 628 HSP 3 Score: 416.387 bits (1069), Expect = 2.526e-120 Identity = 226/529 (42.72%), Postives = 333/529 (62.95%), Query Frame = 1
Query: 574 NIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
N+F+L+ F G+ + F ++ F + + +R+ F+A++RQ++ WFD ++GAL+ +L D V G ++ + Q A GII++F+ GW+LTL+ LS+ P I + ++ K + ++ + AG IA E + +IRT+VS E+ + Y E L + +RK+ + G + M+ SYA F +G+ LI G+ D+++VF + ++ +G A+ + +A+ +AA +F + +R P ID + EG + F+G +TF V F YPTR + P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G VL+D K LNV WLR Q+G+VSQEP+LF CSI ENI YG V +EI +A AN FI LP K +T VGD+GTQLSGGQKQRIAIARAL+R P++LLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I I++GKV+E GTH +L+A G+Y +V+ Q
Sbjct: 749 NMFSLV------FLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGH-MRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSGEGLWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVPHDEIVRAAKEANIHPFIETLPQKYNTRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMVNIQ 1270 HSP 4 Score: 366.311 bits (939), Expect = 9.220e-104 Identity = 230/590 (38.98%), Postives = 346/590 (58.64%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFG------------FLLP-----AMANPN--IEQQSADIRTYVIALAG-IAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+K ++FLG + AI +G+ P I+FG F LP +M NP +E++ Y L G + V A++ + +G Q+ K+ R F A+L+Q++ WFD L TRL+D+ ++I G+ +G AI + A + F W+++LV++A PI+ + + +I+ FS+ + A ++G +A E + IRTV ++ ++ Y++ ++ KKA + G+ I+ +YA F L++ + N F +++ GA ++G++ F + AA +F+++D P I+SFS G D N+ G+++ +V FSYP R NI IL ++ + S QT+ALVG SGCGKST + LLQR YD G I ID I++ N++ LR++ +VSQEP LFS +I ENI YG T D+I A K +N +DFI+ LP +++T VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE VQ ALDKA+EGRT +VIAHRLSTI+NAD I F+DG I E G+H EL+ K GIY +++++Q+
Sbjct: 50 DKLFMFLGTLMAIAHGSGLPLMMIVFGEMTDKFVDNTGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLVAAYIQVSFWTLA---AGRQI-KKIRQKFFHAILRQEMGWFDIKGTT--ELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPD--NIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGN--VTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFRLVNMQT 629
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Mouse
Match: Abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:MGI Symbol;Acc:MGI:1924956]) HSP 1 Score: 502.671 bits (1293), Expect = 1.263e-149 Identity = 260/577 (45.06%), Postives = 370/577 (64.12%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMIS 4036
K+ L+ E ++ LG +++ NG+V P FSI+FG L+ + N D Y + L + +VA V+ ++Q + +G + E L R R F+AML QD+AW+D+ NN GALTT L+ + QI+G + LG V + +M ++ ISF + W+++L++L+F P++ V G++Q M GF+N DK AL+ +GKIA E +ENIRTV +LTRE F Q Y+E ++ K+AH G Y ++ A + A+AA F YL+ +FI F A+ +GAM +G+++ AP + K K A+H+F LL P INS S G D G+++ V F YP RP + +L ++S +I +T+A VG SGCGKST + LLQRFYD + G +L+D +K++N+Q LR +IVSQEP LF+ SI ENIAYGD++++ ++I A A+NIH FI LP +Y T VG +G SGGQKQR+AIARALLR P+ILLLDEATSALD ESEK+VQ ALDKA+ G+TC+V+AHRLSTIQNAD I+V ++G I E GTH ELL Y ++++
Sbjct: 679 LLKIFKLSKSEWPFVVLGTLASALNGSVHPVFSIIFGKLVTMFEDKNKATLKQDAELYSMMLVVLGIVALVTYLMQGLFYGRAEENLAMRLRHSAFKAMLYQDMAWYDDKENNTGALTTTLAVDVAQIQGAATSRLGIVTQDVSNMSLSILISFIYGWEMTLLILSFAPVLAVTGMIQTAAMAGFANRDKQALKRAGKIATEAVENIRTVVSLTRERAFEQMYEETLQTQHRNALKRAHITGCCYAVSHAFVHFAHAAGFRFGAYLIQAGRMMPEGMFIVFTAIAYGAMAIGETLVWAPEYSKAKAGASHLFALLKNKPTINSCSQSGEKPD--TCEGNLEFREVSFVYPCRPEVPVLQNMSLSIEKGKTVAFVGSSGCGKSTCVQLLQRFYDPMKGQVLLDGVDVKELNVQWLRSQTAIVSQEPVLFNCSIAENIAYGDNSRMVPLEEIKEVADAANIHSFIEGLPRKYNTLVGLRGVQLSGGQKQRLAIARALLRKPKILLLDEATSALDNESEKVVQQALDKARRGKTCLVVAHRLSTIQNADMIVVLQNGSIKEQGTHQELLRNGDTYFKLVA 1253 HSP 2 Score: 499.59 bits (1285), Expect = 1.476e-148 Identity = 280/626 (44.73%), Postives = 390/626 (62.30%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGN-GFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRRERR 2184
++F +A DIV+M I+ S+ G T PL+ LV G + + + T++ QN S T E+I+ + TL + GI A I YV ++F+VIT+ Q + IR FF +++ Q+I WFD + L ++ DIN + GIGDKI M Q + F G++++ ++ WKL+LV LS SPLI+ + S+ ++ LT K +AY KAG +AEE L SI+T+ +F ++ E+ RYT++L +A GI+++ + LG V M G+Y L FWYG SLI G G+ +G +L VFFS + ++ IG PH+ +F AR AA IF++ID+ P ID + G +G I FK V F+YP+R +L GL+L I+ G+TVALVG SG GKS+ +QL+QR YD +G + +D+ +I+ NV REQIGVV QEPVLFG +I NI++GR V ++E++QA ANA FI A P K +TLVG++G Q+SGGQKQRIAIARALVRNPKIL+LDEATSALD+ESE +VQ AL+K +GRTTI+VAHRLST+R A +I +KDG V E+GTHAELMA GLY L Q + ++ E R
Sbjct: 36 EIFRFADNLDIVLMTLGILASMINGATVPLMSLVLGEISDHLINGCLVQTNRTKY---QN---CSQTQEKLNEDII-------------VLTLY------YIGIGAAALIFGYVQISFWVITAARQTTRIRKQFFHSILAQDISWFDGSDICELNTRMTGDINKLCDGIGDKIPLMFQNISGFSIGLVISLIKSWKLSLVVLSTSPLIMASSALCSRMIISLTSKELDAYSKAGAVAEEALSSIQTVTAFGAQEKEIQRYTQHLKDAKDAGIKRATASKLSLGAVYFFMNGAYGLAFWYGTSLIFGGEPGYTIGTILAVFFSVIHSSYCIGSVAPHLETFTVARGAAFNIFQVIDKKPNIDNFSTAGFVPECIEGNIEFKNVSFSYPSRPSAKVLKGLNLKIKAGETVALVGPSGSGKSTTVQLLQRLYDPEDGCITVDENDIRAQNVRHYREQIGVVRQEPVLFGTTIGNNIKFGREGVGEKEMEQAAREANAYDFIMAFPKKFNTLVGEKGAQMSGGQKQRIAIARALVRNPKILILDEATSALDTESESLVQTALEKASKGRTTIVVAHRLSTIRGADLIVTMKDGMVVEKGTHAELMAKQGLYYSLAMAQDIKKVDEQMESR 636 HSP 3 Score: 390.193 bits (1001), Expect = 7.985e-112 Identity = 210/512 (41.02%), Postives = 321/512 (62.70%), Query Frame = 1
Query: 619 IALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQ--NSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG---RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
+AL ++M + F+ ++ +R++ F+A++ Q++ W+D + N+GALT L D+ + ++G + Q + I+++F+ GW++TL+ LS +P++ + ++ + M + +A +AG IA E + +IRT+VS E+ Y E L + ++++ +TG + + ++A GF +GA LI+ G G M IVF + IG+ +++A++ A+ +F ++ P I+ ++ G+K + +G + F+ V F YP R + P+L +SLSI+ G+TVA VG+SGCGKS+ +QL+QRFYD +G+VL+D ++K+LNV WLR Q +VSQEPVLF CSI ENI YG RM V EEI + AN +FI LP K +TLVG RG QLSGGQKQR+AIARAL+R PKILLLDEATSALD+ESEK+VQ ALDK G+T ++VAHRLST++NA +I +++G ++EQGTH EL+ Y LV
Sbjct: 746 VALVTYLMQGL---FYGRAEENLAMRLRHSAFKAMLYQDMAWYDDKENNTGALTTTLAVDVAQIQGAATSRLGIVTQDVSNMSLSILISFIYGWEMTLLILSFAPVLAVTGMIQTAAMAGFANRDKQALKRAGKIATEAVENIRTVVSLTRERAFEQMYEETLQTQHRNALKRAHITGCCYAVSHAFVHFAHAAGFRFGAYLIQAGRMMPEG-MFIVFTAIAYGAMAIGETLVWAPEYSKAKAGASHLFALLKNKPTINSCSQSGEKPDTCEGNLEFREVSFVYPCRPEVPVLQNMSLSIEKGKTVAFVGSSGCGKSTCVQLLQRFYDPMKGQVLLDGVDVKELNVQWLRSQTAIVSQEPVLFNCSIAENIAYGDNSRM-VPLEEIKEVADAANIHSFIEGLPRKYNTLVGLRGVQLSGGQKQRLAIARALLRKPKILLLDEATSALDNESEKVVQQALDKARRGKTCLVVAHRLSTIQNADMIVVLQNGSIKEQGTHQELLRNGDTYFKLV 1252 HSP 4 Score: 329.331 bits (843), Expect = 1.045e-91 Identity = 209/586 (35.67%), Postives = 323/586 (55.12%), Query Frame = 2
Query: 2354 LGCVSAIFNGAVQPSFSILFGFLLPAMANPNI---------------EQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL-----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 4051
LG ++++ NGA P S++ G + + N + E+ + DI + GI A + +Q + + + T R R F ++L QDI+WFD ++I L TR++ + ++ G+ + + I + IS W++SLVVL+ P+I L R++ ++ + A ++G +A E + +I+TV+ + IQ Y + +K K+A A G + AY F L+ Y + + VF S +I + +G F + AA ++F+++D+ P I++FST G V E + G+I+ +NV FSYP RP+ +L ++ I + +T+ALVGPSG GKST + LLQR YD +G I +D N I+ N+++ R+ +V QEP LF +I NI +G + ++ AA+ +N +DFI+ P ++ T VGEKG+ SGGQKQRIAIARAL+RNP+IL+LDEATSALDTESE +VQ AL+KA +GRT +V+AHRLSTI+ AD I+ KDG + E GTH EL++K G+Y + Q K
Sbjct: 51 LGILASMINGATVPLMSLVLGEISDHLINGCLVQTNRTKYQNCSQTQEKLNEDIIVLTLYYIGIGAAALIFGYVQISFWVITAARQTTRIRKQFFHSILAQDISWFD--GSDICELNTRMTGDINKLCDGIGDKIPLMFQNISGFSIGLVISLIKSWKLSLVVLSTSPLIMASSALCSRMIISLTSKELDAYSKAGAVAEEALSSIQTVTAFGAQEKEIQRYTQHLKDAKDAGIKRATASKLSLGAVYFFMNGAYGLAFWYGTSLIFGGEPGYTIGTILAVFFS---VIHSSYCIGSVAPHLETFTVARGAAFNIFQVIDKKPNIDNFSTAGFV--PECIEGNIEFKNVSFSYPSRPSAKVLKGLNLKIKAGETVALVGPSGSGKSTTVQLLQRLYDPEDGCITVDENDIRAQNVRHYREQIGVVRQEPVLFGTTIGNNIKFGREG--VGEKEMEQAAREANAYDFIMAFPKKFNTLVGEKGAQMSGGQKQRIAIARALVRNPKILILDEATSALDTESESLVQTALEKASKGRTTIVVAHRLSTIRGADLIVTMKDGMVVEKGTHAELMAKQGLYYSLAMAQDIK 627
BLAST of Multidrug resistance protein 1-like protein vs. UniProt/SwissProt
Match: sp|P21448|MDR1_CRIGR (ATP-dependent translocase ABCB1 OS=Cricetulus griseus OX=10029 GN=ABCB1 PE=1 SV=2) HSP 1 Score: 568.155 bits (1463), Expect = 3.938e-171 Identity = 299/612 (48.86%), Postives = 399/612 (65.20%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+F YA D + M+ + +I GV PL++LVFG+ T A+ + ++ T+ + +S +EE + Y +T GI + I++Y+ V+F+ + + QI IR FF A+M Q IGWFD + G L +L DD++ ++ GIGDKIG Q A F GG I+ F RGWKLTLV L+ISP++ + + +K + T K +AY KAG +AEEVL +IRT+++F G+K E+ RY NL+EA ++GI+K+I +G L++ SYAL FWYG SL+ + +G +L VFF+ L+ IGQA+P++ +F AR AA EIF IID P ID ++ G K ++ +G + FK + F+YP+R D IL GL+L +Q GQTVALVG SGCGKS+ +QL+QR YD EG V ID +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP+K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI++AHRLSTVRNA +I G G + EQG H ELM G+Y LV Q
Sbjct: 38 MFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLVFGDMTDSFAS----VGNIPTNATNNATQVNASDIFGKLEEEMTTYAYYYT------------------GIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKE-YSIGQVLTVFFAVLIGAFSIGQASPNIEAFANARGAAYEIFNIIDNKPSIDSFSKNGYKPDNIKGNLEFKNIHFSYPSRKDVQILKGLNLKVQSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADIIAGFDGGVIVEQGNHEELMREKGIYFKLVMTQ 626 HSP 2 Score: 558.14 bits (1437), Expect = 1.125e-167 Identity = 274/581 (47.16%), Postives = 401/581 (69.02%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA-NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F++++ LN+ E Y +G AI NGA+QP+FSI+F ++ N + E + D + + + V++F++ LQ FG +GE LTKR R + F++ML+QD++WFD P N GALTTRL+++A Q++G +G L + I ++G + IS + WQ++L++LA VPII + G+++++++ G + DK LE SGKIA E IEN RTV +LTRE F Y ++++ KKAH G + TQA ++ +YAA F YL+ + NV + F A++FGAM +G+ + AP++ K K++A+H+ ++++VP I+S+ST G L + G++K V F+YP RP+I +L ++ + QT+ALVG SGCGKST++ LL+RFYD + G + +D ++ +N+Q LR IVSQEP LF SI ENIAYGD++++ + D+I AAK +NIH FI +LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+V ++GK+ E GTH +LL++ GIY M+S+Q+
Sbjct: 694 FWRILKLNSSEWPYFVVGIFCAIVNGALQPAFSIIFSKVVGVFTRNTDDETKRHDSNLFSLLFLILGVISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDNPKNTTGALTTRLANDAGQVKGATGARLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFENMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVARELMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSASHIIMIIEKVPSIDSYSTGG--LKPNTLEGNVKFNEVVFNYPTRPDIPVLQGLNLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEVNQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQA 1272 HSP 3 Score: 405.601 bits (1041), Expect = 6.252e-116 Identity = 229/543 (42.17%), Postives = 334/543 (61.51%), Query Frame = 1
Query: 538 RNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLI-REGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
RN SN+F+L+ F + + FI ++ F + +R F++++RQ++ WFD +GALT +L +D V G ++ + Q A GII++ + GW+LTL+ L+I P+I I ++ K + K + +G IA E + + RT+VS R +K+E Y ++L + ++K+ + G +M+ SYA F +GA L+ RE F ++L+VF + + +GQ + + +A+ +A+ I II++VP ID + G K N +G + F V F YPTR D P+L GL+L ++ GQT+ALVG+SGCGKS+++QL++RFYD G V +D + LNV WLR +G+VSQEP+LF CSI ENI YG V+ +EI++A AN FI +LP+K +T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I I++GKV+E GTH +L+A G+Y +V Q
Sbjct: 738 RNTDDETKRHDSNLFSLL------FLILGVISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDNPKNTTGALTTRLANDAGQVKGATGARLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFE-NMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVARELMTF--ENVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSASHIIMIIEKVPSIDSYSTGGLKPNTLEGNVKFNEVVFNYPTRPDIPVLQGLNLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEVNQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1271 HSP 4 Score: 369.777 bits (948), Expect = 4.748e-104 Identity = 222/590 (37.63%), Postives = 339/590 (57.46%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFL---------LPAMANPNIEQQSA---------DIRTYVIALAGIAVVAFVSSVLQNVGFG--NSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
++ Y+ +G ++AI +G P ++FG + +P A N Q +A ++ TY GI + + +Q V F +G Q+ K R F A++ Q+I WFD +++G L TRL+D+ ++I G+ +G A+ + I F W+++LV+LA P++ + + +I+ F++ + A ++G +A E + IRTV + ++ Y +++ KKA G I+ +YA F L++ + V F A++ GA ++G++ F + AA +F ++D P I+SFS +G D N+ G+++ +N+ FSYP R ++ IL ++ + S QT+ALVG SGCGKST + LLQR YD G + ID I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +VIAHRLST++NAD I F G I E G H EL+ + GIY +++ Q+
Sbjct: 46 DRLYMLVGTLAAIIHGVALPLMMLVFGDMTDSFASVGNIPTNATNNATQVNASDIFGKLEEEMTTYAYYYTGIGAGVLIVAYIQ-VSFWCLAAGRQIHK-IRQKFFHAIMNQEIGWFD--VHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFAVLIGAFSIGQASPNIEAFANARGAAYEIFNIIDNKPSIDSFSKNGYKPD--NIKGNLEFKNIHFSYPSRKDVQILKGLNLKVQSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREN--VTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADIIAGFDGGVIVEQGNHEELMREKGIYFKLVMTQT 627
BLAST of Multidrug resistance protein 1-like protein vs. UniProt/SwissProt
Match: sp|P21447|MDR1A_MOUSE (ATP-dependent translocase ABCB1 OS=Mus musculus OX=10090 GN=Abcb1a PE=1 SV=3) HSP 1 Score: 563.148 bits (1450), Expect = 1.927e-169 Identity = 297/612 (48.53%), Postives = 400/612 (65.36%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+F YA D + M+ + +I GV PL++L+FG+ T A+ + ++++ T+ +E +EE + Y +T GI + I++Y+ V+F+ + + QI IR FF A+M Q IGWFD + G L +L DD++ ++ GIGDKIG Q A F GG I+ F RGWKLTLV L+ISP++ + + +K + T K AY KAG +AEEVL +IRT+++F G+K E+ RY NL+EA ++GI+K+I +G L++ SYAL FWYG SL+ + +G +L VFFS L+ +GQA+P++ +F AR AA E+F+IID P ID ++ G K ++ QG + FK + F+YP+R + IL GL+L ++ GQTVALVG SGCGKS+ +QLMQR YD +G V ID +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR DVT +EI++A+ ANA FI LP++ DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI++AHRLSTVRNA VI G G + EQG H ELM G+Y LV Q
Sbjct: 38 MFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLIFGDMTDSFAS----VGNVSKNSTNMSEA-DKRAMFAKLEEEMTTYAYYYT------------------GIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKE-YSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQ 625 HSP 2 Score: 556.984 bits (1434), Expect = 2.655e-167 Identity = 273/585 (46.67%), Postives = 408/585 (69.74%), Query Frame = 2
Query: 2300 AGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMAN---PNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
A F++++ LN+ E Y +G AI NG +QP+FS++F ++ N P ++Q++++ + + + GI ++F++ LQ FG +GE LTKR R + F++ML+QD++WFD+P N GALTTRL+++A Q++G +G L + I ++G + IS + WQ++L++LA VPII + G+++++++ G + DK LE SGKIA E IEN RTV +LTRE F Y ++++ KKAH G + TQA ++ +YAA F YL+ + NV + F A++FGAM +G+ + AP++ K ++A+H+ ++++ P I+S+ST G L + G+++ V F+YP RP+I +L +S + QT+ALVG SGCGKST++ LL+RFYD + G++ +D +IK +N+Q LR IVSQEP LF SI ENIAYGD++++ + ++I+ AAK +NIH FI +LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+V ++GK+ E GTH +LL++ GIY M+S+Q+
Sbjct: 691 ASFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSVIFSKVVGVFTNGGPPETQRQNSNLFSLLFLILGI--ISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVTQQLMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQG--LKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQA 1271 HSP 3 Score: 409.068 bits (1050), Expect = 5.052e-117 Identity = 228/532 (42.86%), Postives = 332/532 (62.41%), Query Frame = 1
Query: 568 SSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+SN+F+L+ F + + FI ++ F + +R F++++RQ++ WFD +GALT +L +D V G ++ + Q A GII++ + GW+LTL+ L+I P+I I ++ K + K + +G IA E + + RT+VS R +K+E Y ++L + ++K+ + G +M+ SYA F +GA L+ + ++L+VF + + +GQ + + +A +A+ I II++ PEID + +G K N +G + F V F YPTR P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G V +D IK LNV WLR Q+G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI++LP+K +T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I I++GKV+E GTH +L+A G+Y +V Q
Sbjct: 747 NSNLFSLL------FLILGIISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETM-YAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVTQ-QLMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1270 HSP 4 Score: 365.54 bits (937), Expect = 1.243e-102 Identity = 223/589 (37.86%), Postives = 341/589 (57.89%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMAN-PNIEQQS-----ADIR-----------TYVIALAGIAVVAFVSSVLQNVGFG--NSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
++ Y+ +G ++AI +G P ++FG + + A+ N+ + S AD R TY GI + + +Q V F +G Q+ K R F A++ Q+I WFD +++G L TRL+D+ ++I G+ +G A+ + I F W+++LV+LA P++ + + +I+ F++ + A ++G +A E + IRTV + ++ Y +++ KKA G I+ +YA F L++ + V F +++ GA ++G++ F + AA +F+++D P I+SFS G D N+ G+++ +N+ FSYP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD L+G + ID I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP Q++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +VIAHRLST++NAD I F G I E G H EL+ + GIY +++ Q+
Sbjct: 46 DRLYMLVGTLAAIIHGVALPLMMLIFGDMTDSFASVGNVSKNSTNMSEADKRAMFAKLEEEMTTYAYYYTGIGAGVLIVAYIQ-VSFWCLAAGRQIHK-IRQKFFHAIMNQEIGWFD--VHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPD--NIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRED--VTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQT 626
BLAST of Multidrug resistance protein 1-like protein vs. UniProt/SwissProt
Match: sp|P06795|MDR1B_MOUSE (ATP-dependent translocase ABCB1 OS=Mus musculus OX=10090 GN=Abcb1b PE=1 SV=1) HSP 1 Score: 563.148 bits (1450), Expect = 2.316e-169 Identity = 303/616 (49.19%), Postives = 404/616 (65.58%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
GV +F YA D + MI + +I G PL++LVFGN T + S+ +T+Q+ P +ST I +S+S++ M+ + + GI + I++Y+ V+ + + + QI IR FF A+M Q IGWFD + G L +L DD++ ++ GIGDKIG Q F+ G I+ F+ GWKLTLV L++SPLI + + +K + T K +AY KAG +AEEVL +IRT+++F G++ E+ RY +NL+EA +GI+K+I +G+ L++ SYAL FWYG SL+ N + +G++L VFFS L+ T IG P++ +F AR AA EIF+IID P ID + +G K + G + FK V F YP+R++ IL GL+L ++ GQTVALVG SGCGKS+ +QLMQR YD EG V ID +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR DVT +EI++A+ ANA FI LP++ DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI++AHRLSTVRNA VI G G + EQG H ELM G+Y LV Q
Sbjct: 34 GVFGMFRYADWLDKLCMILGTLAAIIHGTLLPLLMLVFGNMTDSFTKAE---ASILPSITNQSGP--NSTLI---------------ISNSSLEEEMAIYAYYYTGIGAGVLIVAYIQVSLWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINDGIGDKIGMFFQSITTFLAGFIIGFISGWKLTLVILAVSPLIGLSSALWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQQKELERYNKNLEEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLS-NEYSIGEVLTVFFSILLGTFSIGHLAPNIEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPDSIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQ 628 HSP 2 Score: 540.421 bits (1391), Expect = 1.676e-161 Identity = 263/577 (45.58%), Postives = 397/577 (68.80%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA-NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMI 4033
F++++ LN E Y+ +G + A+ NG +QP F+I+F ++ + + + E + + + + + +++FV+ Q FG +GE LTKR R + F++ML+QDI+WFD+ N+ G+LTTRL+ +A+ ++G G L V + ++G V +S + WQ++L+++ +P+I +GGI++++++ G + DK LE SGKIA E IEN RT+ +LTRE F Y ++++ KKAH G + TQA ++ +YAA F YL+ + NV + F A++FGAM G + + AP++ K K++A+H+ ++++ P I+S+ST+G L + G++K V+F+YP RPNI +L +S + QT+ALVG SGCGKST++ LL+RFYD + G++ +D +IK +N+Q LR IVSQEP LF SI ENIAYGD+++ + ++I+ AAK +NIH FI +LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+V ++GK+ E GTH +LL++ GIY M+
Sbjct: 695 FWRILNLNLSEWPYLLVGVLCAVINGCIQPVFAIVFSRIVGVFSRDDDHETKRQNCNLFSLFFLVMGLISFVTYFFQGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKGAMGARLAVVTQNVANLGTGVILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKQLEISGKIATEAIENFRTIVSLTREQKFETMYAQSLQVPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAQQLMTFENVMLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEG--LKPTLLEGNVKFNGVQFNYPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMV 1269 HSP 3 Score: 405.601 bits (1041), Expect = 7.728e-116 Identity = 226/525 (43.05%), Postives = 331/525 (63.05%), Query Frame = 1
Query: 610 FAGIALAMFIMSYVSVTF--FVITSQHQI--SAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNR 2157
F+ L M ++S+V+ F F +I +R F++++RQ+I WFD ++G+LT +L D ++V +G ++ + Q A G+IL+ V GW+LTL+ + I PLI++ ++ K + K + +G IA E + + RTIVS R +K+E Y ++L + ++K+ + G +M+ SYA F +GA L+ + ++++VF + + G + + +A+ +A+ I II++ PEID + EG K +G + F V+F YPTR + P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G V +D IK LNV WLR +G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI++LP+K +T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I I++GKV+E GTH +L+A G+Y +V R
Sbjct: 753 FSLFFLVMGLISFVTYFFQGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKGAMGARLAVVTQNVANLGTGVILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKQLEISGKIATEAIENFRTIVSLTREQKFETM-YAQSLQVPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAQ-QLMTFENVMLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEGLKPTLLEGNVKFNGVQFNYPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMVQAGAKR 1275 HSP 4 Score: 376.711 bits (966), Expect = 2.823e-106 Identity = 230/598 (38.46%), Postives = 350/598 (58.53%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFL-----------LPAMAN---PN---------IEQQSADIRTYVIAL-AGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPN---FGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
+K + LG ++AI +G + P ++FG + LP++ N PN +E++ A Y + AG+ +VA++ L + +G Q+ K R F A++ Q+I WFD +++G L TRL+D+ ++I G+ +G +I + A I F W+++LV+LA P+I + L +++ F+N + A ++G +A E + IRTV + ++ Y + +++ KKA G+ ++ +YA F L+L N + V F +++ G ++G +APN F + AA +F+++D P I+SFST G D ++ G+++ +NV F+YP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD L G + ID I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP Q++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +VIAHRLST++NAD I F G I E G H EL+ + GIY +++ Q+
Sbjct: 46 DKLCMILGTLAAIIHGTLLPLLMLVFGNMTDSFTKAEASILPSITNQSGPNSTLIISNSSLEEEMAIYAYYYTGIGAGVLIVAYIQVSLWCLA---AGRQIHK-IRQKFFHAIMNQEIGWFD--VHDVGELNTRLTDDVSKINDGIGDKIGMFFQSITTFLAGFIIGFISGWKLTLVILAVSPLIGLSSALWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQQKELERYNKNLEEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLSNEYSIGEVLTVFFSILLGTFSIGH---LAPNIEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPD--SIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRED--VTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTR 630
BLAST of Multidrug resistance protein 1-like protein vs. UniProt/SwissProt
Match: sp|P21449|MDR2_CRIGR (Multidrug resistance protein 2 OS=Cricetulus griseus OX=10029 GN=PGY2 PE=2 SV=2) HSP 1 Score: 563.148 bits (1450), Expect = 2.551e-169 Identity = 302/616 (49.03%), Postives = 401/616 (65.10%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G+ +F YA D + M+ + ++ G + PL++LVFGN T +S T+ TS +T+ + I + E I S ++ M+ + + GI + I++Y+ V+F+ + + QI+ IR FF A+M Q IGWFD + G L +L DD++ ++ GIGDKIG Q A F+ I+ F+ GWKLTLV L++SPLI + M +K + T K +AY KAG +AEEVL +IRT+++F G+ E+ RY +NL+EA +GI+K++ +G+ L++ SYAL FWYG SL+ N + VG +L VFFS L T IG P++ F AR AA EIF+IID P ID + +G K + G + FK V F+YP+R+ IL GL+L +Q GQTVALVG SGCGKS+ +QL+QR YD EG V ID +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP+K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK EGRTTI++AHRLSTVRNA VI G G + EQG H ELM G+Y LV Q
Sbjct: 35 GIFGMFRYADWLDKLYMVLGTLAAVLHGTSLPLLMLVFGNMT----------DSFTKAETSIWPNMTNQSEINNTEVI-----------SGSLEEDMATYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQINKIRQKFFHAIMNQEIGWFDVHDIGELNTRLTDDVSKINDGIGDKIGMFFQSIATFLAAFIVGFISGWKLTLVILAVSPLIGLSSAMWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQNKELERYNKNLEEAKNVGIKKAVTANISIGIAYLLVYASYALAFWYGTSLVLS-NEYSVGQVLTVFFSILFGTFSIGHIAPNIEVFANARGAAYEIFKIIDNEPSIDSFSTQGHKPDSVMGNLEFKNVHFSYPSRSGIKILKGLNLKVQSGQTVALVGKSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHEELMKEKGIYCRLVMMQ 628 HSP 2 Score: 534.643 bits (1376), Expect = 1.582e-159 Identity = 264/579 (45.60%), Postives = 392/579 (67.70%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA---NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMI 4033
F+ ++ LN E Y+ +G + A+ NG +QP FSI+F ++ +P +QQ+ ++ + + G+ + FV+ Q FG +GE LTKR R + F++ML+QDI+WFD+ N+ GALTTRL+ +A ++G L + + ++G + IS + WQ++L+++ P+I + G+++++++ G + DK LE SGKIA E IEN RTV +LTRE F Y ++++ KKAH G + TQA ++ +YAA F YL+ + + NV + F A++FGA+ G + + AP++ K K++A+H+ +++++P I+S+ST G L + G++K V F+YP RP+I +L +S + QT+ALVG SGCGKST++ LL+RFYD + G + +D +IK +N+Q LR IVSQEP LF SI ENIAYGD++++ + D+I AAK +NIH FI +LPD+Y T+VG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+V ++GK+ E GTH +LL++ GIY M+
Sbjct: 695 FWGILKLNITEWPYLVVGVLCAVINGCMQPVFSIVFSGIIGVFTRDDDPKTKQQNCNLFSLFFLVMGM--ICFVTYFFQGFTFGKAGEILTKRLRYMVFKSMLRQDISWFDDHRNSTGALTTRLASDAANVKGAMSSRLAGITQNVANLGTGIIISLVYGWQLTLLLVVIAPLIILSGMMEMKVLSGQALKDKKELEVSGKIATEAIENFRTVVSLTREQKFENMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAHQIMTFENVMLVFSAVVFGAIAAGNASSFAPDYAKAKVSASHIIRIMEKIPSIDSYSTRG--LKPNWLEGNVKFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMV 1269 HSP 3 Score: 402.519 bits (1033), Expect = 6.665e-115 Identity = 218/492 (44.31%), Postives = 314/492 (63.82%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFDAQ--NSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNR 2157
+R F++++RQ+I WFD ++GALT +L D V + ++ + Q A GII++ V GW+LTL+ + I+PLII+ +M K + K + +G IA E + + RT+VS R +K+E Y ++L + ++K+ + G +M+ SYA F +GA L+ ++++VF + + G A+ + +A+ +A+ I I++++P ID + G K N +G + F V F YPTR D P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G V +D IK LNV WLR +G+VSQEP+LF CSI ENI YG V+ +EI++A AN FI +LP+K +T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I I++GKV+E GTH +L+A G+Y +V R
Sbjct: 786 LRYMVFKSMLRQDISWFDDHRNSTGALTTRLASDAANVKGAMSSRLAGITQNVANLGTGIIISLVYGWQLTLLLVVIAPLIILSGMMEMKVLSGQALKDKKELEVSGKIATEAIENFRTVVSLTREQKFE-NMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAH-QIMTFENVMLVFSAVVFGAIAAGNASSFAPDYAKAKVSASHIIRIMEKIPSIDSYSTRGLKPNWLEGNVKFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVQAGAKR 1275 HSP 4 Score: 385.571 bits (989), Expect = 3.346e-109 Identity = 231/595 (38.82%), Postives = 347/595 (58.32%), Query Frame = 2
Query: 2336 EKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMAN------PNIEQQSA-------------DIRTYVIALAGIAVVAFVSSVLQNVGFG--NSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPN---FGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
+K Y+ LG ++A+ +G P ++FG + + PN+ QS D+ TY GI + + +Q V F +G Q+ K R F A++ Q+I WFD ++IG L TRL+D+ ++I G+ +G +I + AA + F W+++LV+LA P+I + + +++ F+N + A ++G +A E + IRTV ++ ++ Y + +++ KKA G+ ++ +YA F L+L N + V F +++FG ++G +APN F + AA +F+++D P I+SFST G D ++ G+++ +NV FSYP R I IL ++ + S QT+ALVG SGCGKST + LLQR YD G + ID I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA+EGRT +VIAHRLST++NAD I F G I E G H EL+ + GIY +++ +Q+
Sbjct: 47 DKLYMVLGTLAAVLHGTSLPLLMLVFGNMTDSFTKAETSIWPNMTNQSEINNTEVISGSLEEDMATYAYYYTGIGAGVLIVAYIQ-VSFWCLAAGRQINK-IRQKFFHAIMNQEIGWFD--VHDIGELNTRLTDDVSKINDGIGDKIGMFFQSIATFLAAFIVGFISGWKLTLVILAVSPLIGLSSAMWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQNKELERYNKNLEEAKNVGIKKAVTANISIGIAYLLVYASYALAFWYGTSLVLSNEYSVGQVLTVFFSILFGTFSIGH---IAPNIEVFANARGAAYEIFKIIDNEPSIDSFSTQGHKPD--SVMGNLEFKNVHFSYPSRSGIKILKGLNLKVQSGQTVALVGKSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREN--VTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHEELMKEKGIYCRLVMMQTR 630
BLAST of Multidrug resistance protein 1-like protein vs. UniProt/SwissProt
Match: sp|P08183|MDR1_HUMAN (ATP-dependent translocase ABCB1 OS=Homo sapiens OX=9606 GN=ABCB1 PE=1 SV=3) HSP 1 Score: 562.377 bits (1448), Expect = 4.292e-169 Identity = 299/615 (48.62%), Postives = 403/615 (65.53%), Query Frame = 1
Query: 304 VGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
V +F Y+ D + M+ + +I G PL++LVFG T A +L +++ IT+ + I D F + N+ M+ + ++GI + + +Y+ V+F+ + + QI IR FF A+MRQ IGWFD + G L +L DD++ ++ GIGDKIG Q A F G I+ F RGWKLTLV L+ISP++ + + +K + T K AY KAG +AEEVL +IRT+++F G+K E+ RY +NL+EA +IGI+K+I +G L++ SYAL FWYG +L+ G + +G +L VFFS L+ +GQA+P + +F AR AA EIF+IID P ID ++ G K ++ +G + F+ V F+YP+R + IL GL+L +Q GQTVALVG SGCGKS+ +QLMQR YD EG V +D +I+ +NV +LRE IGVVSQEPVLF +I ENIRYGR +VT +EI++A+ ANA FI LP+K DTLVG+RG QLSGGQKQRIAIARALVRNPKILLLDEATSALD+ESE +VQ ALDK +GRTTI++AHRLSTVRNA VI G DG + E+G H ELM G+Y LV Q
Sbjct: 36 VFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFAN----AGNLEDLMSN----ITNRSDINDT---------GFFM---NLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGE-YSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQ 629 HSP 2 Score: 553.518 bits (1425), Expect = 6.014e-166 Identity = 271/583 (46.48%), Postives = 408/583 (69.98%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA---NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
F+++M LN E Y +G AI NG +QP+F+I+F ++ +P ++Q++++ + + + +++F++ LQ FG +GE LTKR R + F +ML+QD++WFD+P N GALTTRL+++A Q++G G L + I ++G + ISF + WQ++L++LA VPII + G+++++++ G + DK LE SGKIA E IEN RTV +LT+E F Y ++++ +KAH G + TQA ++ +YA F YL+ + + +V + F A++FGAM +G+ + AP++ K KI+AAH+ ++++ P I+S+ST+G++ + + G++ V F+YP RP+I +L +S + QT+ALVG SGCGKST++ LL+RFYD L G +L+D +IK +N+Q LR IVSQEP LF SI ENIAYGD++++ + ++I+ AAK +NIH FI +LP++Y TKVG+KG+ SGGQKQRIAIARAL+R P ILLLDEATSALDTESEK+VQ+ALDKA+EGRTC+VIAHRLSTIQNAD I+VF++G++ E GTH +LL++ GIY M+S+Q+
Sbjct: 697 FWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNL--FSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPN--TLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQA 1275 HSP 3 Score: 418.313 bits (1074), Expect = 3.795e-120 Identity = 235/540 (43.52%), Postives = 333/540 (61.67%), Query Frame = 1
Query: 568 SSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRR 2175
+SN+F+L+ F + + FI ++ F + +R FR+++RQ++ WFD +GALT +L +D V IG ++ + Q A GII++F+ GW+LTL+ L+I P+I I ++ K + K + +G IA E + + RT+VS E+ Y ++L + +RK+ + G +M+ SYA F +GA L+ D+L+VF + + +GQ + + +A+ +AA I II++ P ID + EG N +G +TF V F YPTR D P+L GLSL ++ GQT+ALVG+SGCGKS+++QL++RFYD G+VL+D IK LNV WLR +G+VSQEP+LF CSI ENI YG V+ EEI +A AN FI +LPNK T VGD+GTQLSGGQKQRIAIARALVR P ILLLDEATSALD+ESEK+VQ+ALDK EGRT I++AHRLST++NA +I ++G+V+E GTH +L+A G+Y +V Q TKR+
Sbjct: 751 NSNLFSLL------FLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAH-KLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAG---TKRQ 1280 HSP 4 Score: 371.318 bits (952), Expect = 1.535e-104 Identity = 222/595 (37.31%), Postives = 344/595 (57.82%), Query Frame = 2
Query: 2327 NAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANP--------NIEQQS------------ADIRTYVIALAGIAVVAFVSSVLQNVGFG--NSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
N +K Y+ +G ++AI +GA P ++FG + AN NI +S D+ Y +GI V++ +Q V F +G Q+ K R F A+++Q+I WFD +++G L TRL+D+ ++I G+ +G ++ + + F W+++LV+LA P++ + + +I+ F++ + A ++G +A E + IRTV + ++ Y + +++ KKA G I+ +YA F L+L + V F +++ GA ++G++ F + AA +F+++D P I+S+S G D N+ G+++ NV FSYP R + IL ++ + S QT+ALVG SGCGKST + L+QR YD G + +D I+ IN++ LR++ +VSQEP LF+ +I ENI YG T D+I A K +N +DFI+ LP +++T VGE+G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE +VQ ALDKA++GRT +VIAHRLST++NAD I F DG I E G H EL+ + GIY +++++Q+
Sbjct: 44 NWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQ-VSFWCLAAGRQIHK-IRKQFFHAIMRQEIGWFD--VHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPD--NIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGREN--VTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQT 630
BLAST of Multidrug resistance protein 1-like protein vs. TrEMBL
Match: A0A0H3YJ53 (Slc24a-6 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc24a-6 PE=2 SV=1) HSP 1 Score: 657.522 bits (1695), Expect = 0.000e+0 Identity = 357/364 (98.08%), Postives = 360/364 (98.90%), Query Frame = -1
Query: 6410 KKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLESICEVLHLRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDELVHWYEGXXXXXXXXXXXXXXYKNDSVYQIVVKLLPISKRKLDPEKKPLFYENDSLAKSLIXXXXXXXXXXXXXXXNFQTNKRIHGCDNEDSIEGDKLESMFAIPDSFVSRLHWVTMLPVTVLCFLTIPDCRRPGIYKKLFFLTFLMSLVWITICIYLLVWMICITGE 7501
KKHK+FLKIILCYFSLIFGSIF LKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLE ICEVLH+RHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDE VHWYEGLIMLIIYSLYILLLYKNDSVYQIVVKLLPISKRKLDPEKKPLFYENDSLAK LIDSENDDDLEKEDLNSNFQTNKRIHGCDNEDSIEGDKLESMFAIPDSFVSRLHWVTMLPVTVLCFLTIPDCRRPGIYKKLFFLTFLMSLVWITICIYLLVWMICITG+
Sbjct: 1 KKHKNFLKIILCYFSLIFGSIFLLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLERICEVLHMRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDEQVHWYEGLIMLIIYSLYILLLYKNDSVYQIVVKLLPISKRKLDPEKKPLFYENDSLAKILIDSENDDDLEKEDLNSNFQTNKRIHGCDNEDSIEGDKLESMFAIPDSFVSRLHWVTMLPVTVLCFLTIPDCRRPGIYKKLFFLTFLMSLVWITICIYLLVWMICITGD 364
BLAST of Multidrug resistance protein 1-like protein vs. TrEMBL
Match: A8WG28 (Zgc:172149 protein OS=Danio rerio OX=7955 GN=abcb4 PE=2 SV=1) HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0 Identity = 299/620 (48.23%), Postives = 411/620 (66.29%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQ---FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRP 2160
+LF YA DI++M+ +IMS+ G PL+++VFG+ T + T + +++ I NFT ++ TL M+ ++ + + + +Y+ V F+ + + Q+ +R FF ++M+Q IGWFD +G L +L DD+ ++ GIGDK+G ++Q FI GII+ F +GWKLTLV L++SPL+ I ++ K M T K AY KAG +AEEVL SIRT+ +F G+K E+ RY +NL++A +G+RK+I +G +++ SYAL FWYG++LI G + +G +L +FF+ L+ G+GQ +P++ +F+ AR AA ++F+IID P+I+ +EEG KL+ +G I FK + F YP+R D +L G++L + GQT+ALVG+SGCGKS+ IQL+QRFYD EG V ID +I+ LNV LRE IGVVSQEPVLF +I ENIRYGR DVT +EI+QA ANA FI LP+K +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTTI+VAHRLST+RNA VI G ++G++ E GTH ELM G+Y LV+ Q P
Sbjct: 43 ELFRYADSIDILLMMLGLIMSMANGAVLPLMVIVFGDMT---------------------DSFVDDTLLDNLKNITLPP--NFTFPETSNITLGEKMTTHAIYYSIMGFVVLVAAYMQVAFWTLAAGRQVKKLRKIFFHSIMKQEIGWFDVNETGQLNTRLTDDVYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKEQTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYMSYALAFWYGSTLILGGE-YTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQIIDHEPKINSFSEEGYKLDVVKGNIEFKNINFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCGKSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYGRQDVTQDEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQAALDKVRLGRTTIVVAHRLSTIRNADVIAGFQNGEIVELGTHDELMERKGIYHSLVNMQVRNP 638 HSP 2 Score: 391.349 bits (1004), Expect = 1.091e-114 Identity = 227/588 (38.61%), Postives = 347/588 (59.01%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFG-----------------------FLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
+ LG + ++ NGAV P I+FG F P +N + ++ + T+ I + + V V++ +Q + + + K+ R I F +++KQ+I WFD N G L TRL+D+ +I G+ LG +I + + + I F W+++LV+LA P++ + + ++M F++ ++ A ++G +A E + +IRTV + I+ Y + ++ +KA G T I+ +YA F L+L + + F A++ GA LG++ F + AA +F+++D P INSFS +G LDV + G+I+ +N+ F YP R ++ +LN ++ + S QTIALVG SGCGKST I LLQRFYD G++ ID + I+ +N++ LR+L +VSQEP LF+ +I ENI YG T D+I AA+ +N ++FI+ LPD++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD ESE IVQ ALDK + GRT +V+AHRLSTI+NAD I F++G+I E GTH EL+ + GIY ++++Q
Sbjct: 56 MMLGLIMSMANGAVLPLMVIVFGDMTDSFVDDTLLDNLKNITLPPNFTFPETSNITLGEK---MTTHAIYYSIMGFVVLVAAYMQVAFWTLAAGRQVKKLRKIFFHSIMKQEIGWFD--VNETGQLNTRLTDDVYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKEQTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYMSYALAFWYGSTLILGGEYTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQIIDHEPKINSFSEEGYKLDV--VKGNIEFKNINFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCGKSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYGRQD--VTQDEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQAALDKVRLGRTTIVVAHRLSTIRNADVIAGFQNGEIVELGTHDELMERKGIYHSLVNMQ 634
BLAST of Multidrug resistance protein 1-like protein vs. TrEMBL
Match: A0A4W4E7J0 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 OS=Electrophorus electricus OX=8005 PE=4 SV=1) HSP 1 Score: 568.926 bits (1465), Expect = 0.000e+0 Identity = 298/642 (46.42%), Postives = 414/642 (64.49%), Query Frame = 1
Query: 316 FSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRRERRDLC*C**RNYQRKSYKKTL 2241
F YA D+V+M IM++ G P +++VFG+ T+ ++N + + NE + S E + R+ Y +++M G+ + +Y+ V F+ + + Q+ +R FF A+++Q+IGWFD +G L +L DDI ++ GIGDK+G ++Q F G I+ F++GWKLTLV L+ISPL+ I +++K M T + AY KAG +AEEVL +IRT+ +F G++ E+ RY +NL+EA +GI+K++ +G +++ +YAL FWYG++LI G + +G +L VFF+ ++ GIGQ +P++ +F AR AA ++F IID P ID +++G K + +G I F+ ++FTYP+R D +L+G+SL + GQT+ALVG+SGCGKS+ +QL+QRFYD EG V ID +I+ LNV +LRE IGVVSQEPVLF +I ENIRYGR DVT EEI QA ANA FI LP K +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTTIIVAHRLST+RNA +I G + G++ E GTH +LM G+Y LV Q R ++ E+ C + R KTL
Sbjct: 28 FRYADGGDLVLMFFGTIMAMANGAVLPAMVIVFGDMTNS------FVNE--EIINGWNETVNQSLG----ESMTRHAIY---------YSIMGGI----------VLFAAYMQVAFWTLAAGRQVKRLRKMFFHAIIKQDIGWFDVNETGQLNTRLADDIYKINEGIGDKMGMLIQSITTFFAGFIVGFIKGWKLTLVILAISPLLGISAAIIAKVMTSFTTREQTAYAKAGAVAEEVLSAIRTVFAFGGQRKEIQRYQKNLEEAKNVGIKKAVTVNIAVGFTYFMIYMAYALAFWYGSTLIFAGE-YSIGSLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHEPNIDSFSDQGYKPDVVKGNIEFRNIQFTYPSRQDVKVLSGMSLKVSCGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVCIDGHDIRTLNVRYLRELIGVVSQEPVLFATTIAENIRYGREDVTQEEIQQAAREANAYDFIMKLPQKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADIIAGFQHGRIVELGTHDQLMQKKGVYHSLVTMQVRRSVSQYTEK-----CWKKQLHRPGASKTL 632 HSP 2 Score: 523.087 bits (1346), Expect = 0.000e+0 Identity = 263/581 (45.27%), Postives = 395/581 (67.99%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA--NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F V+GLN E Y+ +G A NG +QP+F+I+F ++ A + +I+++ A++ + + A+ G V++F++ LQ FG +GE LT R R F+AM++QD+ W+D+P N++GALTTRL+++ Q++G +G + + + ++G ++ ISF + WQ++L+VL+ VPI+ V G +Q++++ G + DK LE +GK+A E IENIRTV +LTRE F +++ + KKAH GF + +QA I+ AYA F +L M VF+ A+++GAM +G++ + PN+ K K++A+H+ L+++V IN+ S +G E+ SG++++++V FSYP RP + +L +S + QT+ALVG SGCGKSTII LL+RFYD ++D + K +NI LR IVSQEP LF S+ ENIAYGD+++ +++ AA+A+NIH FI LP +Y+T+ G+KG+ SGGQKQRIAIARA+LRNP++LLLDEATSALDTESEK+VQ+ALDKA+EGRTC+V+AHRLSTIQNAD I V K+G + E GTH +LLS+ G Y +++ Q
Sbjct: 660 FLTVLGLNKKEWPYMVVGVFCATINGGLQPAFAIIFSKIIAVFAEMDEDIKRERANLFSLLFAVIG--VISFIALFLQGFCFGKAGEILTMRLRYRAFKAMMRQDLGWYDDPKNSVGALTTRLANDTAQVQGATGLRMATLAQNVANLGTSIIISFVYGWQLTLLVLSIVPIMAVAGAIQMKMLAGHALKDKKELELAGKVATEAIENIRTVVSLTRESKFESLFQQNLIVPYKNAQKKAHIFGFTFSFSQAMIYFAYAGCFKFGSWLYEKEMITFEGVFLVISAIMYGAMAVGEANSFTPNYAKAKMSASHVLMLINKVSAINNDSDEGE--QPEHFSGNVRLQDVHFSYPSRPAVPVLQGLSLRVKKGQTLALVGSSGCGKSTIIQLLERFYDP-RQGAMLDIHDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVAPEEVEQAARAANIHSFIEGLPQKYQTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKVVQEALDKAREGRTCIVVAHRLSTIQNADVIAVLKNGVLVEQGTHQQLLSQQGAYYALVNSQ 1235 HSP 3 Score: 392.504 bits (1007), Expect = 2.842e-109 Identity = 226/548 (41.24%), Postives = 339/548 (61.86%), Query Frame = 1
Query: 520 DMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+M+E ++ ++ +N+F+L+ FA I + FI ++ F + +R F+A+MRQ++GW+D +NS GALT +L +D V G ++ + Q A II++FV GW+LTL+ LSI P++ + + K + K + AG +A E + +IRT+VS R K+E + + +NL K +K+ + G +++ +YA F +G S + E + +V + + +G+A ++ +A+ +A+ + +I++V I+ ++EG++ F G + + V F+YP+R P+L GLSL ++ GQT+ALVG+SGCGKS++IQL++RFYD +G +L D + K LN+ WLR QIG+VSQEPVLF CS+ ENI YG V EE++QA AN +FI LP K T GD+GTQLSGGQKQRIAIARA++RNPK+LLLDEATSALD+ESEK+VQ+ALDK EGRT I+VAHRLST++NA VI +K+G + EQGTH +L++ G Y LV+ Q
Sbjct: 704 EMDEDIKRER-------ANLFSLL------FAVIGVISFIALFLQGFCFGKAGEILTMRLRYRAFKAMMRQDLGWYDDPKNSVGALTTRLANDTAQVQGATGLRMATLAQNVANLGTSIIISFVYGWQLTLLVLSIVPIMAVAGAIQMKMLAGHALKDKKELELAGKVATEAIENIRTVVSLTRESKFE-SLFQQNLIVPYKNAQKKAHIFGFTFSFSQAMIYFAYAGCFKFG-SWLYEKEMITFEGVFLVISAIMYGAMAVGEANSFTPNYAKAKMSASHVLMLINKVSAINNDSDEGEQPEHFSGNVRLQDVHFSYPSRPAVPVLQGLSLRVKKGQTLALVGSSGCGKSTIIQLLERFYDPRQGAML-DIHDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVAPEEVEQAARAANIHSFIEGLPQKYQTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKVVQEALDKAREGRTCIVVAHRLSTIQNADVIAVLKNGVLVEQGTHQQLLSQQGAYYALVNSQ 1235 HSP 4 Score: 390.963 bits (1003), Expect = 8.346e-109 Identity = 223/581 (38.38%), Postives = 344/581 (59.21%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNI---------EQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK*IVQ 4063
+F G + A+ NGAV P+ I+FG + + N I + + + I + + + ++ +Q + + + KR R + F A++KQDI WFD N G L TRL+D+ +I G+ +G +I +I + A + F W+++LV+LA P++ + + ++M F+ ++ A ++G +A E + IRTV + IQ Y++ +++ KKA G T I+ AYA F L+ + ++ F A+I GA +G++ F + AA +F ++D P I+SFS G DV + G+I+ N++F+YP R ++ +L+ +S ++ QTIALVG SGCGKST + LLQRFYD G + ID + I+ +N++ LR+L +VSQEP LF+ +I ENI YG T ++I AA+ +N +DFI+ LP ++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE IVQ ALDK + GRT +++AHRLSTI+NAD I F+ G+I E GTH +L+ K G+Y ++++Q + + Q
Sbjct: 39 MFFGTIMAMANGAVLPAMVIVFGDMTNSFVNEEIINGWNETVNQSLGESMTRHAIYYSIMGGIVLFAAYMQVAFWTLAAGRQVKRLRKMFFHAIIKQDIGWFD--VNETGQLNTRLADDIYKINEGIGDKMGMLIQSITTFFAGFIVGFIKGWKLTLVILAISPLLGISAAIIAKVMTSFTTREQTAYAKAGAVAEEVLSAIRTVFAFGGQRKEIQRYQKNLEEAKNVGIKKAVTVNIAVGFTYFMIYMAYALAFWYGSTLIFAGEYSIGSLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHEPNIDSFSDQGYKPDV--VKGNIEFRNIQFTYPSRQDVKVLSGMSLKVSCGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVCIDGHDIRTLNVRYLRELIGVVSQEPVLFATTIAENIRYGRED--VTQEEIQQAAREANAYDFIMKLPQKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADIIAGFQHGRIVELGTHDQLMQKKGVYHSLVTMQVRRSVSQ 613
BLAST of Multidrug resistance protein 1-like protein vs. TrEMBL
Match: A0A4W4E811 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 OS=Electrophorus electricus OX=8005 PE=4 SV=1) HSP 1 Score: 558.525 bits (1438), Expect = 0.000e+0 Identity = 302/663 (45.55%), Postives = 420/663 (63.35%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNE--PITSSTS---------IPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAM-----FIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRRERRDLC*C**RNYQRKSYKKTLCSQ 2250
++F YA D+V+M IM++ G P +++VFG+ T+ ++N + + NE P+ S S + ++E +R Y++ + S L G I ++ +Y+ V F+ + + Q+ +R FF A+++Q+IGWFD +G L +L DDI ++ GIGDK+G ++Q F G I+ F++GWKLTLV L+ISPL+ I +M S T + AY KAG +AEEVL +IRT+ +F G++ E+ RY +NL+EA +GI+K++ +G +++ +YAL FWYG++LI G + +G +L VFF+ ++ GIGQ +P++ +F AR AA ++F IID P ID +++G K + +G I F+ ++FTYP+R D +L+G+SL + GQT+ALVG+SGCGKS+ +QL+QRFYD EG V ID +I+ LNV +LRE IGVVSQEPVLF +I ENIRYGR DVT EEI QA ANA FI LP K +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTTIIVAHRLST+RNA +I G + G++ E GTH +LM G+Y LV Q R ++ Y K +KK +CS
Sbjct: 27 EIFRYADGGDLVLMFFGTIMAMANGAVLPAMVIVFGDMTNS------FVNE--EIINGWNESVPLPPSESYIFPGQGLSVLELECYIR---YDWCYAPS----LHLGPPPHRHAIYYSIMGGIVLFAAYMQVAFWTLAAGRQVKRLRKMFFHAIIKQDIGWFDVNETGQLNTRLADDIYKINEGIGDKMGMLIQSITTFFAGFIVGFIKGWKLTLVILAISPLLGISAVMTSFTT-----REQTAYAKAGAVAEEVLSAIRTVFAFGGQRKEIQRYQKNLEEAKNVGIKKAVTVNIAVGFTYFMIYMAYALAFWYGSTLIFAGE-YSIGSLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHEPNIDSFSDQGYKPDVVKGNIEFRNIQFTYPSRQDVKVLSGMSLKVSCGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVCIDGHDIRTLNVRYLRELIGVVSQEPVLFATTIAENIRYGREDVTQEEIQQAAREANAYDFIMKLPQKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADIIAGFQHGRIVELGTHDQLMQKKGVYHSLVTMQVRRSVSQ--------------YTEKCWKKHVCSH 654 HSP 2 Score: 492.656 bits (1267), Expect = 0.000e+0 Identity = 254/579 (43.87%), Postives = 384/579 (66.32%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F V+GLN E Y+ +G A NG +QP+F+I+F ++ P+I+++ A++ + + A+ I V++F++ LQ FG +GE LT R R F+AM++QD+ W+D+P N++GALTTRL+++ Q++G +G + + + ++G ++ ISF + WQ++L+VL+ VPI+ V G +Q++++ G + DK LE +GK+ +T H++ + G KKAH GF + +QA I+ AYA F +L M VF+ A+++GAM +G++ + PN+ K K++A+H+ L+++V IN+ S +G E+ SG++++++V FSYP RP + +L +S + QT+ALVG SGCGKSTII LL+RFYD G +++D + K +NI LR IVSQEP LF S+ ENIAYGD+++ +++ AA+A+NIH FI LP QY+T+ G+KG+ SGGQKQRIAIARA+LRNP++LLLDEATSALDTESEK+VQ+ALDKA+EGRTC+V+AHRLSTIQNAD I V K+G + E GTH +LLS+ G Y +++ Q
Sbjct: 677 FLTVLGLNKKEWPYMVVGVFCATINGGLQPAFAIIFSKIIAV--GPDIKRERANLFSLLFAV--IGVISFIALFLQGFCFGKAGEILTMRLRYRAFKAMMRQDLGWYDDPKNSVGALTTRLANDTAQVQGATGLRMATLAQNVANLGTSIIISFVYGWQLTLLVLSIVPIMAVAGAIQMKMLAGHALKDKKELELAGKVP---------------RYT---HHRHTMAY--GNAQKKAHIFGFTFSFSQAMIYFAYAGCFKFGSWLYEKEMITFEGVFLVISAIMYGAMAVGEANSFTPNYAKAKMSASHVLMLINKVSAINNDSDEGE--QPEHFSGNVRLQDVHFSYPSRPAVPVLQGLSLRVKKGQTLALVGSSGCGKSTIIQLLERFYDPRQGAVMLDIHDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVAPEEVEQAARAANIHSFIEGLP-QYQTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKVVQEALDKAREGRTCIVVAHRLSTIQNADVIAVLKNGVLVEQGTHQQLLSQQGAYYALVNSQ 1228 HSP 3 Score: 375.17 bits (962), Expect = 1.373e-103 Identity = 231/614 (37.62%), Postives = 349/614 (56.84%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNI---EQQSADI---RTYVIALAGIAVV-------------------------AFVSSVL---------QNVGFGN--SGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK*IVQ 4063
+F G + A+ NGAV P+ I+FG + + N I +S + +Y+ G++V+ A S++ V F +G Q+ KR R + F A++KQDI WFD N G L TRL+D+ +I G+ +G +I +I + A + F W+++LV+LA P++ + + M F+ ++ A ++G +A E + IRTV + IQ Y++ +++ KKA G T I+ AYA F L+ + ++ F A+I GA +G++ F + AA +F ++D P I+SFS G DV + G+I+ N++F+YP R ++ +L+ +S ++ QTIALVG SGCGKST + LLQRFYD G + ID + I+ +N++ LR+L +VSQEP LF+ +I ENI YG T ++I AA+ +N +DFI+ LP ++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE IVQ ALDK + GRT +++AHRLSTI+NAD I F+ G+I E GTH +L+ K G+Y ++++Q + + Q
Sbjct: 40 MFFGTIMAMANGAVLPAMVIVFGDMTNSFVNEEIINGWNESVPLPPSESYIFPGQGLSVLELECYIRYDWCYAPSLHLGPPPHRHAIYYSIMGGIVLFAAYMQVAFWTLAAGRQV-KRLRKMFFHAIIKQDIGWFD--VNETGQLNTRLADDIYKINEGIGDKMGMLIQSITTFFAGFIVGFIKGWKLTLVILAISPLLGISAV-----MTSFTTREQTAYAKAGAVAEEVLSAIRTVFAFGGQRKEIQRYQKNLEEAKNVGIKKAVTVNIAVGFTYFMIYMAYALAFWYGSTLIFAGEYSIGSLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHEPNIDSFSDQGYKPDV--VKGNIEFRNIQFTYPSRQDVKVLSGMSLKVSCGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVCIDGHDIRTLNVRYLRELIGVVSQEPVLFATTIAENIRYGRED--VTQEEIQQAAREANAYDFIMKLPQKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADIIAGFQHGRIVELGTHDQLMQKKGVYHSLVTMQVRRSVSQ 641 HSP 4 Score: 369.392 bits (947), Expect = 1.161e-101 Identity = 215/530 (40.57%), Postives = 318/530 (60.00%), Query Frame = 1
Query: 571 SNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+N+F+L+ FA I + FI ++ F + +R F+A+MRQ++GW+D +NS GALT +L +D V G ++ + Q A II++FV GW+LTL+ LSI P++ + + K + K + AG + +Y R+T A K K+ + G +++ +YA F +G S + E + +V + + +G+A ++ +A+ +A+ + +I++V I+ ++EG++ F G + + V F+YP+R P+L GLSL ++ GQT+ALVG+SGCGKS++IQL++RFYD +G V++D + K LN+ WLR QIG+VSQEPVLF CS+ ENI YG V EE++QA AN +FI LP + T GD+GTQLSGGQKQRIAIARA++RNPK+LLLDEATSALD+ESEK+VQ+ALDK EGRT I+VAHRLST++NA VI +K+G + EQGTH +L++ G Y LV+ Q
Sbjct: 727 ANLFSLL------FAVIGVISFIALFLQGFCFGKAGEILTMRLRYRAFKAMMRQDLGWYDDPKNSVGALTTRLANDTAQVQGATGLRMATLAQNVANLGTSIIISFVYGWQLTLLVLSIVPIMAVAGAIQMKMLAGHALKDKKELELAGKV----------------PRYTHHRHTMAYGNAQK----KAHIFGFTFSFSQAMIYFAYAGCFKFG-SWLYEKEMITFEGVFLVISAIMYGAMAVGEANSFTPNYAKAKMSASHVLMLINKVSAINNDSDEGEQPEHFSGNVRLQDVHFSYPSRPAVPVLQGLSLRVKKGQTLALVGSSGCGKSTIIQLLERFYDPRQGAVMLDIHDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVAPEEVEQAARAANIHSFIEGLP-QYQTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKVVQEALDKAREGRTCIVVAHRLSTIQNADVIAVLKNGVLVEQGTHQQLLSQQGAYYALVNSQ 1228
BLAST of Multidrug resistance protein 1-like protein vs. TrEMBL
Match: E9G5N8 (ABC transporter, subfamily ABCB/MDR OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_347264 PE=4 SV=1) HSP 1 Score: 555.058 bits (1429), Expect = 0.000e+0 Identity = 300/637 (47.10%), Postives = 420/637 (65.93%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQM---ATYQYYMNSLTQFLTSQNE-----PITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIR---EGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKRRERRDL 2190
LF YA+ +D V++ + ++G GV +PL++++FG+ T+ Q +N + ++S P+ + +I D E + + F + + I +F++ ++ VT T+++Q+ IR+ F +AV+RQ++GW+D ++S +++ +D+N + G+G+KIG + CFI II AF+ GW+LTLV L +P++ + M +L+K LT+ +AY KAGGIAEEV SIRT+++F G++ E+ R+ ++L A K GI++ + TG GLV G+++ SYAL FWYG +LI +GN + D+LIVFFS L+ IGQA P+M +F+ AR AAA IF IIDRVP ID + EG + GKI+F+ V F YP+R D IL G+S + PGQTVALVG SGCGKS+ IQL+QRFYD EG V ID +++LN+ WLR+Q+G+V QEPVLFG SI ENI YGR V+ EE+++A ANA FI LP K DTLVG+RG QLSGGQKQRIAIARALVR PKILLLDEATSALD++SE +VQ ALDK +GRTTIIVAHRL+T+RNA I +KDG VQE GTH +LMALNG+Y LV Q+ ++E+ ++
Sbjct: 71 LFRYASSTDFVLLCFGTLGAVGTGVCFPLMLILFGDITNAFVGGGMDQETINEINCNISSDPNYTYPFPLGPTCNISDPSEFANSPQGQAVQDEFTKFGIYVAI------IGAVLFLLGFIFVTALNFTAENQVYRIRSKFLQAVLRQDVGWYDTKSSNDFASRITEDLNKIQDGVGEKIGMFIFSMTCFIASIINAFIHGWELTLVMLVSTPVLAVSMGVLAKVQASLTENELKAYAKAGGIAEEVFSSIRTVMAFGGQRKEIDRFQDDLAYAKKAGIKRGMATGIGAGLVWGIIYASYALAFWYGITLILAACDGNSYSSSDLLIVFFSVLIGAMQIGQAAPYMEAFSVARGAAATIFAIIDRVPPIDSSSNEGLVPDGVDGKISFRDVFFNYPSRPDVKILQGISFDVTPGQTVALVGTSGCGKSTCIQLLQRFYDPLEGSVTIDGNELRNLNLGWLRDQMGMVGQEPVLFGTSIGENICYGRDGVSKEEMERAAKEANAHDFIQRLPRKYDTLVGERGGQLSGGQKQRIAIARALVRQPKILLLDEATSALDTQSEAVVQKALDKARQGRTTIIVAHRLTTIRNADRIIVMKDGIVQEDGTHDKLMALNGIYYQLVIAQQGGESDSKKEKEEM 701 HSP 2 Score: 490.73 bits (1262), Expect = 0.000e+0 Identity = 258/583 (44.25%), Postives = 376/583 (64.49%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILF----GFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
+M +N E +I +G + + G P F+ILF G L P + ++ A Y + + +V S+ Q+ F +GE LT R R +TF+A+LKQ+I WFD +N++GAL RLS +A ++G +G +G + A+ +M A+ ++ F W++ LV L FVP++ V Q +I+ G S ++ AL++S K+A+E I NIRTV++L +E F Y E+++ E KK+ +GF++G + AYA T G+L++ T+VF +L+FG +G+++A APN+ K K+AA +F LL RVP I++ S +G+VL EN+ G++ E V+F YP R + +L +S + + QT+ALVG SGCGKST I LL+RFYD +G + +D I +NI +LR IVSQEP LF+ +I +NIAYGD++++ D+II AA+ +NIH FI +LP+ YET VGE+G+ SGGQKQR+AIARAL+RNP+ILLLDEATSALD+ESE +VQ ALD A+EGRTC+ IAHRLSTIQNAD I+V G I+E GTH EL+ G+Y ++ S+Q
Sbjct: 742 LMDIMRMNRKEWHFIVVGVIGSAIVGLSTPVFAILFSEVLGVLTPGGSAEEQAEKRAQGNFYALMFLILGIVVGFSAFAQSFSFSIAGESLTSRLRGLTFQAILKQEIGWFDRKTNSVGALCARLSGDAASVQGATGSRIGVLFQAVTTMIASTVLALYFQWKLGLVALCFVPLLLVSTYFQAKIIMGQSALEREALQKSAKVAMEAISNIRTVASLGKERQFHTIYMESLRGPHKEALKKSWIRGFIFGFASSIPMFAYAVTMYYGGWLVVNECLDFTSVFKVSESLLFGTQMIGQAVAFAPNYNKAKVAANRIFALLRRVPQIDASSNNGLVL--ENVDGNVNFEQVRFRYPTRKDAEVLQGLSLAVRAGQTVALVGHSGCGKSTCIQLLERFYDPDSGQVQLDGQDINPVNISSLRSQMGIVSQEPILFNLTIAQNIAYGDNSRVVPMDEIIEAARKANIHVFIQSLPNGYETMVGERGTQLSGGQKQRVAIARALIRNPKILLLDEATSALDSESEHVVQMALDAAREGRTCITIAHRLSTIQNADNIIVINHGTISEQGTHEELIKLGGLYFELCSVQ 1322 HSP 3 Score: 388.267 bits (996), Expect = 3.135e-107 Identity = 223/500 (44.60%), Postives = 304/500 (60.80%), Query Frame = 1
Query: 664 FVITSQHQISAIRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLI-REGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
F I + S +R F+A+++Q IGWFD + + GAL +L D +V G +IG + Q I +LA WKL LV L PL+++ +K +M + EA K+ +A E + +IRT+ S E+ Y E+L K ++KS + G + G + +YA+ +YG L+ E F + V S L T IGQA ++N+A+ AA IF ++ RVP+ID + G L + G + F+ V+F YPTR D +L GLSL+++ GQTVALVG SGCGKS+ IQL++RFYD + G+V +D +I +N+ LR Q+G+VSQEP+LF +I +NI YG V +EI +A AN FI +LPN +T+VG+RGTQLSGGQKQR+AIARAL+RNPKILLLDEATSALDSESE +VQ ALD EGRT I +AHRLST++NA I I G + EQGTH EL+ L GLY +L Q
Sbjct: 825 FSIAGESLTSRLRGLTFQAILKQEIGWFDRKTNSVGALCARLSGDAASVQGATGSRIGVLFQAVTTMIASTVLALYFQWKLGLVALCFVPLLLVSTYFQAKIIMGQSALEREALQKSAKVAMEAISNIRTVASLGKERQFHTIYMESLRGPHKEALKKSWIRGFIFGFASSIPMFAYAVTMYYGGWLVVNECLDFT--SVFKVSESLLFGTQMIGQAVAFAPNYNKAKVAANRIFALLRRVPQIDASSNNGLVLENVDGNVNFEQVRFRYPTRKDAEVLQGLSLAVRAGQTVALVGHSGCGKSTCIQLLERFYDPDSGQVQLDGQDINPVNISSLRSQMGIVSQEPILFNLTIAQNIAYGDNSRVVPMDEIIEAARKANIHVFIQSLPNGYETMVGERGTQLSGGQKQRVAIARALIRNPKILLLDEATSALDSESEHVVQMALDAAREGRTCITIAHRLSTIQNADNIIVINHGTISEQGTHEELIKLGGLYFELCSVQ 1322 HSP 4 Score: 365.155 bits (936), Expect = 1.152e-99 Identity = 212/522 (40.61%), Postives = 320/522 (61.30%), Query Frame = 2
Query: 2507 VAFVSSVLQNVGF------GNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLY----NMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
VA + +VL +GF + E R RS +A+L+QD+ W+D S+N +R++++ +I+ GE +G I ++ A++ +F W+++LV+L P++ V + ++ + ++ A ++G IA E +IRTV + I +++ + K+ A G GL I+ +YA F L+L N +++ I F +++ GAM +G++ F + AAA +F ++DRVPPI+S S +G+V D + G I +V F+YP RP++ IL +SF++ QT+ALVG SGCGKST I LLQRFYD L G++ ID N+++++N+ LR +V QEP LF SI ENI YG + +++ AAK +N HDFI LP +Y+T VGE+G SGGQKQRIAIARAL+R P+ILLLDEATSALDT+SE +VQ ALDKA++GRT +++AHRL+TI+NAD+I+V KDG + E GTH +L++ NGIY Q++ Q
Sbjct: 172 VAIIGAVLFLLGFIFVTALNFTAENQVYRIRSKFLQAVLRQDVGWYDTKSSN--DFASRITEDLNKIQDGVGEKIGMFIFSMTCFIASIINAFIHGWELTLVMLVSTPVLAVSMGVLAKVQASLTENELKAYAKAGGIAEEVFSSIRTVMAFGGQRKEIDRFQDDLAYAKKAGIKRGMATGIGAGLVWGIIYASYALAFWYGITLILAACDGNSYSSSDLLIVFFSVLIGAMQIGQAAPYMEAFSVARGAAATIFAIIDRVPPIDSSSNEGLVPD--GVDGKISFRDVFFNYPSRPDVKILQGISFDVTPGQTVALVGTSGCGKSTCIQLLQRFYDPLEGSVTIDGNELRNLNLGWLRDQMGMVGQEPVLFGTSIGENICYGRDG--VSKEEMERAAKEANAHDFIQRLPRKYDTLVGERGGQLSGGQKQRIAIARALVRQPKILLLDEATSALDTQSEAVVQKALDKARQGRTTIIVAHRLTTIRNADRIIVMKDGIVQEDGTHDKLMALNGIYYQLVIAQ 687
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Cavefish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414]) HSP 1 Score: 547.74 bits (1410), Expect = 4.145e-165 Identity = 290/619 (46.85%), Postives = 402/619 (64.94%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQ--MATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASL-IREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G +LF YATC +++MM+ + + G+T P++ +VFG T ++ Q + L F T+ + + +IP ++ I MS + F + A+ ++ VT F++T+ Q IR +F AV+ Q + WFD G L +L DDINT++ G+GDKI +Q++ F+ G I+ F+ GWKLTLV +S+SPL+ + SK + LT K AY KAG +AEE+L +IRT+V+F G++ +Y +NL A G++K++ T +GL +FG+YAL FWYG L + + + +G ++IVFFS ++ +GQ P++ S +AR AA I+ ID ID +EEG K + +G I F+ V F+YP+R D IL G+SL + G+T+ALVGASGCGKS+ IQL+QRFYD GEV +D +I+ LNV WLRE +G+VSQEPVLFG +I ENIRYGR D TD++ID+A+ ANA FI+ LP+ L+T+VG+RG QLSGGQKQRIAIARALV+NPKILLLDEATSALD++SE IVQ ALDK GRTTI++AHRLST+R A VI G KDG+V EQGTH ELM+ G+Y LV +Q
Sbjct: 5 GFFQLFRYATCLEVLMMVLGLGCAALHGITLPIMCVVFGQMTDSFVLSGQQLNLTGLGNFSTNLS---SGCVAIPGID----------------IEASMSQHAYYFVALGAAVLLLGTFQVTLFLLTATRQTKRIREKYFHAVLHQPMAWFDTHPIGELNTRLTDDINTINDGLGDKICIFVQFFCTFLAGFIIGFIYGWKLTLVIMSVSPLLAASAAVWSKILATLTSKELSAYAKAGAVAEEILVAIRTVVAFNGQQKAAEKYEKNLITAKDFGVKKAVTTNVSMGLTQFFIFGAYALAFWYGTKLTVDDPVNYSIGKVIIVFFSVMIGAFSLGQGAPNLESVAKARGAAYSIYNTIDMPRPIDSSSEEGYKPDSVRGDIEFRNVHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPEAGEVTLDGRDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSEAIVQAALDKARAGRTTIVIAHRLSTIRTADVIAGFKDGEVVEQGTHGELMSKKGVYHSLVMQQ 604 HSP 2 Score: 493.426 bits (1269), Expect = 2.152e-146 Identity = 248/583 (42.54%), Postives = 380/583 (65.18%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVE-NMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ-SH 4048
F +++ LN E Y+ +G +++ G V P+ +ILF ++ + P+ E + + + + VAF++ Q FG SGE LT R RS F+A+L+QDI+WFD+ +N +G LTT+L+ +A+ ++G +G LG IC++ A+ ++F WQ++L++LA PI+ +Q+R M G ++ D+ ALE SG+I+ E +EN +TV +LT+E F + + +++ K KA G + L QA + AA F +L+ + ++ NVF+ F ++F AM++G+S + AP+F K K AA + LL++ P I+ +S G D N GDI+ +V F YP RPNI IL ++ ++ QT+ALVG SGCGKST I LL+RFY+ G + D + +N+ LR +VSQEP LF +I ENI YGD++++ + ++++ AA ++NIH+FI +LP++Y T+VG+KG+ SGGQKQRIAIARAL+R P++LLLDEATSALDTESEKIVQ ALD+A++GRTC+VIAHRL+T+QNAD I V ++G++ E G H EL++K+G Y +++ Q SH
Sbjct: 689 FSRILALNKSEWPYLLVGTLASFVGGGVYPAVAILFAKIIGVFSEPDPEVKRQKALMFSLLFLLVGAVAFLTFFFQGYMFGKSGEILTMRLRSQAFKAILRQDISWFDDHNNAVGVLTTKLATDASLVKGAAGSRLGLATNTICALVIAMIVAFFHSWQLTLMILACTPILTGANFIQMRAMAGHTSKDQSALEMSGRISTETVENFKTVVSLTKEDVFFRKFNDSLNKPYRSALCKAPIYGITFALAQAIPYFVNAAVFRFGSWLIAHCHTQFENVFLVFSVIVFAAMSIGQSSSFAPDFAKAKAAAGRILALLEKTPEIDIYSEAG---DKPINFKGDIEFRDVHFFYPTRPNIKILQGLNVSVTQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRGLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVVEAAISANIHNFISDLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKVLLLDEATSALDTESEKIVQKALDEARKGRTCIVIAHRLTTVQNADIIAVIQNGQVVEQGMHSELMAKHGAYYALVNAQVSH 1268 HSP 3 Score: 390.963 bits (1003), Expect = 5.390e-112 Identity = 226/550 (41.09%), Postives = 334/550 (60.73%), Query Frame = 1
Query: 511 SIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
S PD E+ R K F+L +F L+ ++ L F Y+ F + + +R+ F+A++RQ+I WFD N+ G LT KL D + V G ++G + +I+AF W+LTL+ L+ +P++ + + M T K A +G I+ E + + +T+VS E ++ ++L++ + + K+ + G L + + A F +G+ LI + ++ +VF + A IGQ++ F +A++AA I ++++ PEID +E GDK +F+G I F+ V F YPTR + IL GL++S+ GQT+ALVG SGCGKS+ IQL++RFY+ G+V D + + LN+ WLR Q+G+VSQEP+LF C+I ENI+YG V+ EE+ +A + AN FI+ LP K +T VGD+GTQLSGGQKQRIAIARALVR PK+LLLDEATSALD+ESEKIVQ ALD+ +GRT I++AHRL+TV+NA +I I++G+V EQG H+ELMA +G Y LV+ Q
Sbjct: 732 SEPD-PEVKRQKALMFSL----LFLLVGAVA------FLTFFFQGYM----FGKSGEILTMRLRSQAFKAILRQDISWFDDHNNAVGVLTTKLATDASLVKGAAGSRLGLATNTICALVIAMIVAFFHSWQLTLMILACTPILTGANFIQMRAMAGHTSKDQSALEMSGRISTETVENFKTVVSLTKEDVFFRKFNDSLNKPYRSALCKAPIYGITFALAQAIPYFVNAAVFRFGSWLIAHCHTQF-ENVFLVFSVIVFAAMSIGQSSSFAPDFAKAKAAAGRILALLEKTPEIDIYSEAGDKPINFKGDIEFRDVHFFYPTRPNIKILQGLNVSVTQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRGLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVVEAAISANIHNFISDLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKVLLLDEATSALDTESEKIVQKALDEARKGRTCIVIAHRLTTVQNADIIAVIQNGQVVEQGMHSELMAKHGAYYALVNAQ 1265 HSP 4 Score: 353.214 bits (905), Expect = 1.335e-99 Identity = 221/544 (40.62%), Postives = 313/544 (57.54%), Query Frame = 2
Query: 2429 AMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL-----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
A+ +IE + Y +AL G AV+ Q F + + TKR R F A+L Q +AWFD ++ IG L TRL+D+ I G+ + + C+ A I F + W+++LV+++ P++ + +I+ ++ + A ++G +A E + IRTV + + Y++ + KKA GLTQ IF AYA F L + Y++ K+ VF S ++ GA +LG+ + K + AA ++ +D PI+S S +G D ++ GDI+ NV FSYP R ++ IL +S + +TIALVG SGCGKST I LLQRFYD G + +D I+ +N++ LR+ IVSQEP LF +I ENI YG TD I A + +N ++FI LPD T VGE+G+ SGGQKQRIAIARAL++NP+ILLLDEATSALDT+SE IVQ ALDKA+ GRT +VIAHRLSTI+ AD I FKDG++ E GTH EL+SK G+Y ++ Q+
Sbjct: 74 AIPGIDIEASMSQHAYYFVAL-GAAVLLL--GTFQVTLFLLTATRQTKRIREKYFHAVLHQPMAWFD--THPIGELNTRLTDDINTINDGLGDKICIFVQFFCTFLAGFIIGFIYGWKLTLVIMSVSPLLAASAAVWSKILATLTSKELSAYAKAGAVAEEILVAIRTVVAFNGQQKAAEKYEKNLITAKDFGVKKAVTTNVSMGLTQFFIFGAYALAFWYGTKLTVDDPVNYSIGKVIIVFFS---VMIGAFSLGQGAPNLESVAKARGAAYSIYNTIDMPRPIDSSSEEGYKPD--SVRGDIEFRNVHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPEAGEVTLDGRDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDA--TDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSEAIVQAALDKARAGRTTIVIAHRLSTIRTADVIAGFKDGEVVEQGTHGELMSKKGVYHSLVMQQT 605
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Cavefish
Match: abcb4 (multidrug resistance protein 1 [Source:NCBI gene;Acc:103043081]) HSP 1 Score: 531.946 bits (1369), Expect = 5.596e-162 Identity = 267/579 (46.11%), Postives = 387/579 (66.84%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
FF V+GLN E Y+ LG AI NG +QP+F+I+F ++ AN + + + + A I V+FV+ LQ FG SGE LT R R F AML+QD W+D+P N++GALTTRL+ + Q++G +G + + + ++G ++ ISF + WQ++L++L+ VPI+ V G +Q++++ G + DK LE +GK+A E IENIRTV +LTRE F Y++ + KKAH G + +QA I+ AYA F +L+ M V++ A+++GAM +G++ + PN+ K KI+A+H+ L++RVP I++ S +G E+ G+++ +V F YP RP++ +L + + Q++ALVG SGCGKST I LL+RFYD + G +++D+ K +NI LR IVSQEP LF S+ ENIAYGD+++ T ++I AAKA+NIH FI LP +Y T+ G+KG+ SGGQKQRIAIARA+LRNP++LLLDEATSALDTESEKIVQ+ALDKA+EGRTC+V+AHRLSTIQNAD I VF++G + E GTH +LL++ G+Y +++ Q
Sbjct: 458 FFTVLGLNKTEWPYMVLGVFCAIINGGMQPAFAIIFSKIISVFANLDEDIVRERTNFFSLMFAVIGAVSFVALFLQGYSFGKSGEILTMRLRYRAFRAMLRQDPGWYDDPKNSVGALTTRLATDTAQVQGATGVRMATLAQNVANLGTSIIISFVYGWQLTLLILSIVPIMAVAGAIQMKMLGGHAVKDKKELELAGKVATEAIENIRTVVSLTRESKFEILYQQNLVVPYQNAKKKAHIFGLTFSFSQAMIYFAYAGCFRFGAWLIEQKMMDFEGVYLVISAVMYGAMAVGEANSFTPNYAKAKISASHVLMLINRVPAIDNESEEGE--KPEDFEGNVRFHDVHFKYPSRPDVPVLQGLRLRVKKGQSLALVGSSGCGKSTTIQLLERFYDPMQGAVMLDDKDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVTQEEIERAAKAANIHSFIQELPQKYLTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKIVQEALDKAREGRTCIVVAHRLSTIQNADVIAVFQNGVVVEQGTHQQLLNQQGVYYTLVNSQ 1034 HSP 2 Score: 459.144 bits (1180), Expect = 6.023e-137 Identity = 231/389 (59.38%), Postives = 291/389 (74.81%), Query Frame = 1
Query: 982 KASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
K AY KAG +AEEVL +IRT+ +F G+K E+ RY +NL++A +GIRK+I+ +G V +++ SYAL FWYG++LI + VG++L VFF+ ++ GIGQ +P++ +F AR AA ++F IID P ID +EEG K + +G I F+ + F YP+R D +L G++L I+ GQT+ALVG+SGCGKS+ +QL+QRFYD EG V IDD +I+ +NV LRE IGVVSQEPVLF SI ENIRYGR DVT EEI+QA ANA FI LP+K +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTTIIVAHRLST+RNA VI G ++GK+ E GTH ELM + G+Y+ LV Q
Sbjct: 7 KEQTAYAKAGAVAEEVLSAIRTVFAFGGQKKELKRYQKNLEDAKNVGIRKAIMVNISVGFVYFMIYMSYALAFWYGSTLII-AKEYQVGNLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHKPSIDSFSEEGYKPDVVKGNIEFRNIHFNYPSRQDVKVLNGMNLKIRSGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVTIDDHDIRSMNVRCLRELIGVVSQEPVLFATSIAENIRYGREDVTQEEIEQATREANAYDFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADVIAGFQNGKIVELGTHDELMGMKGVYQSLVTMQ 394 HSP 3 Score: 417.927 bits (1073), Expect = 5.737e-123 Identity = 239/546 (43.77%), Postives = 339/546 (62.09%), Query Frame = 1
Query: 526 EEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
E+IVR + +N F+LM FA I F+ ++ F + + +R FRA++RQ+ GW+D +NS GALT +L D V G ++ + Q A II++FV GW+LTL+ LSI P++ + + K + K + AG +A E + +IRT+VS R K+E+ Y +NL + +K+ + G +++ +YA F +GA LI + G L++ + + +G+A ++ +A+ +A+ + +I+RVP ID +EEG+K DF+G + F V F YP+R D P+L GL L ++ GQ++ALVG+SGCGKS+ IQL++RFYD +G V++DD + K LN+ WLR QIG+VSQEPVLF CS+ ENI YG VT EEI++A AN +FI LP K T GD+GTQLSGGQKQRIAIARA++RNPK+LLLDEATSALD+ESEKIVQ+ALDK EGRT I+VAHRLST++NA VI ++G V EQGTH +L+ G+Y LV+ Q
Sbjct: 505 EDIVRER--------TNFFSLM------FAVIGAVSFVALFLQGYSFGKSGEILTMRLRYRAFRAMLRQDPGWYDDPKNSVGALTTRLATDTAQVQGATGVRMATLAQNVANLGTSIIISFVYGWQLTLLILSIVPIMAVAGAIQMKMLGGHAVKDKKELELAGKVATEAIENIRTVVSLTRESKFEIL-YQQNLVVPYQNAKKKAHIFGLTFSFSQAMIYFAYAGCFRFGAWLIEQKMMDFEGVYLVIS-AVMYGAMAVGEANSFTPNYAKAKISASHVLMLINRVPAIDNESEEGEKPEDFEGNVRFHDVHFKYPSRPDVPVLQGLRLRVKKGQSLALVGSSGCGKSTTIQLLERFYDPMQGAVMLDDKDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVTQEEIERAAKAANIHSFIQELPQKYLTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKIVQEALDKAREGRTCIVVAHRLSTIQNADVIAVFQNGVVVEQGTHQQLLNQQGVYYTLVNSQ 1034 HSP 4 Score: 319.316 bits (817), Expect = 8.296e-90 Identity = 175/399 (43.86%), Postives = 256/399 (64.16%), Query Frame = 2
Query: 2849 MQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
M F++ ++ A ++G +A E + IRTV + ++ Y++ ++ +KA G I+ +YA F L++ ++ N+ F A+I GA +G++ F + AA +F ++D P I+SFS +G DV + G+I+ N+ F+YP R ++ +LN ++ I S QTIALVG SGCGKST + LLQRFYD G + ID++ I+ +N++ LR+L +VSQEP LF+ SI ENI YG T ++I A + +N +DFI+ LPD++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE IVQ ALDK + GRT +++AHRLSTI+NAD I F++GKI E GTH EL+ G+Y ++++Q+
Sbjct: 1 MTSFTSKEQTAYAKAGAVAEEVLSAIRTVFAFGGQKKELKRYQKNLEDAKNVGIRKAIMVNISVGFVYFMIYMSYALAFWYGSTLIIAKEYQVGNLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHKPSIDSFSEEGYKPDV--VKGNIEFRNIHFNYPSRQDVKVLNGMNLKIRSGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVTIDDHDIRSMNVRCLRELIGVVSQEPVLFATSIAENIRYGRED--VTQEEIEQATREANAYDFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADVIAGFQNGKIVELGTHDELMGMKGVYQSLVTMQT 395
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Cavefish
Match: abcb4 (multidrug resistance protein 1 [Source:NCBI gene;Acc:103043081]) HSP 1 Score: 525.783 bits (1353), Expect = 2.547e-160 Identity = 266/579 (45.94%), Postives = 386/579 (66.67%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
FF V+GLN E Y+ LG AI NG +QP+F+I+F ++ + Q+ + + A I V+FV+ LQ FG SGE LT R R F AML+QD W+D+P N++GALTTRL+ + Q++G +G + + + ++G ++ ISF + WQ++L++L+ VPI+ V G +Q++++ G + DK LE +GK+A E IENIRTV +LTRE F Y++ + KKAH G + +QA I+ AYA F +L+ M V++ A+++GAM +G++ + PN+ K KI+A+H+ L++RVP I++ S +G E+ G+++ +V F YP RP++ +L + + Q++ALVG SGCGKST I LL+RFYD + G +++D+ K +NI LR IVSQEP LF S+ ENIAYGD+++ T ++I AAKA+NIH FI LP +Y T+ G+KG+ SGGQKQRIAIARA+LRNP++LLLDEATSALDTESEKIVQ+ALDKA+EGRTC+V+AHRLSTIQNAD I VF++G + E GTH +LL++ G+Y +++ Q
Sbjct: 431 FFTVLGLNKTEWPYMVLGVFCAIINGGMQPAFAIIFSKIISVG-----QVQTERTNFFSLMFAVIGAVSFVALFLQGYSFGKSGEILTMRLRYRAFRAMLRQDPGWYDDPKNSVGALTTRLATDTAQVQGATGVRMATLAQNVANLGTSIIISFVYGWQLTLLILSIVPIMAVAGAIQMKMLGGHAVKDKKELELAGKVATEAIENIRTVVSLTRESKFEILYQQNLVVPYQNAKKKAHIFGLTFSFSQAMIYFAYAGCFRFGAWLIEQKMMDFEGVYLVISAVMYGAMAVGEANSFTPNYAKAKISASHVLMLINRVPAIDNESEEGE--KPEDFEGNVRFHDVHFKYPSRPDVPVLQGLRLRVKKGQSLALVGSSGCGKSTTIQLLERFYDPMQGAVMLDDKDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVTQEEIERAAKAANIHSFIQELPQKYLTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKIVQEALDKAREGRTCIVVAHRLSTIQNADVIAVFQNGVVVEQGTHQQLLNQQGVYYTLVNSQ 1002 HSP 2 Score: 460.685 bits (1184), Expect = 7.180e-138 Identity = 235/413 (56.90%), Postives = 300/413 (72.64%), Query Frame = 1
Query: 919 ISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNR 2157
+ PL+ C+ T K AY KAG +AEEVL +IRT+ +F G+K E+ RY +NL++A +GIRK+I+ +G V +++ SYAL FWYG++LI + VG++L VFF+ ++ GIGQ +P++ +F AR AA ++F IID P ID +EEG K + +G I F+ + F YP+R D +L G++L I+ GQT+ALVG+SGCGKS+ +QL+QRFYD EG V IDD +I+ +NV LRE IGVVSQEPVLF SI ENIRYGR DVT EEI+QA ANA FI LP+K +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTTIIVAHRLST+RNA VI G ++GK+ E GTH ELM + G+Y+ LV Q+ +
Sbjct: 16 VCPLLCFCVFKSVMTSFTS--KEQTAYAKAGAVAEEVLSAIRTVFAFGGQKKELKRYQKNLEDAKNVGIRKAIMVNISVGFVYFMIYMSYALAFWYGSTLII-AKEYQVGNLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHKPSIDSFSEEGYKPDVVKGNIEFRNIHFNYPSRQDVKVLNGMNLKIRSGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVTIDDHDIRSMNVRCLRELIGVVSQEPVLFATSIAENIRYGREDVTQEEIEQATREANAYDFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADVIAGFQNGKIVELGTHDELMGMKGVYQSLVTMQEEK 425 HSP 3 Score: 415.616 bits (1067), Expect = 1.757e-122 Identity = 235/531 (44.26%), Postives = 333/531 (62.71%), Query Frame = 1
Query: 571 SNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSF-RGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+N F+LM FA I F+ ++ F + + +R FRA++RQ+ GW+D +NS GALT +L D V G ++ + Q A II++FV GW+LTL+ LSI P++ + + K + K + AG +A E + +IRT+VS R K+E+ Y +NL + +K+ + G +++ +YA F +GA LI + G L++ + + +G+A ++ +A+ +A+ + +I+RVP ID +EEG+K DF+G + F V F YP+R D P+L GL L ++ GQ++ALVG+SGCGKS+ IQL++RFYD +G V++DD + K LN+ WLR QIG+VSQEPVLF CS+ ENI YG VT EEI++A AN +FI LP K T GD+GTQLSGGQKQRIAIARA++RNPK+LLLDEATSALD+ESEKIVQ+ALDK EGRT I+VAHRLST++NA VI ++G V EQGTH +L+ G+Y LV+ Q
Sbjct: 480 TNFFSLM------FAVIGAVSFVALFLQGYSFGKSGEILTMRLRYRAFRAMLRQDPGWYDDPKNSVGALTTRLATDTAQVQGATGVRMATLAQNVANLGTSIIISFVYGWQLTLLILSIVPIMAVAGAIQMKMLGGHAVKDKKELELAGKVATEAIENIRTVVSLTRESKFEIL-YQQNLVVPYQNAKKKAHIFGLTFSFSQAMIYFAYAGCFRFGAWLIEQKMMDFEGVYLVIS-AVMYGAMAVGEANSFTPNYAKAKISASHVLMLINRVPAIDNESEEGEKPEDFEGNVRFHDVHFKYPSRPDVPVLQGLRLRVKKGQSLALVGSSGCGKSTTIQLLERFYDPMQGAVMLDDKDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSRQVTQEEIERAAKAANIHSFIQELPQKYLTQAGDKGTQLSGGQKQRIAIARAILRNPKLLLLDEATSALDTESEKIVQEALDKAREGRTCIVVAHRLSTIQNADVIAVFQNGVVVEQGTHQQLLNQQGVYYTLVNSQ 1002 HSP 4 Score: 321.627 bits (823), Expect = 6.876e-91 Identity = 176/402 (43.78%), Postives = 257/402 (63.93%), Query Frame = 2
Query: 2846 IMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 4051
+M F++ ++ A ++G +A E + IRTV + ++ Y++ ++ +KA G I+ +YA F L++ ++ N+ F A+I GA +G++ F + AA +F ++D P I+SFS +G DV + G+I+ N+ F+YP R ++ +LN ++ I S QTIALVG SGCGKST + LLQRFYD G + ID++ I+ +N++ LR+L +VSQEP LF+ SI ENI YG T ++I A + +N +DFI+ LPD++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALDTESE IVQ ALDK + GRT +++AHRLSTI+NAD I F++GKI E GTH EL+ G+Y ++++Q K
Sbjct: 28 VMTSFTSKEQTAYAKAGAVAEEVLSAIRTVFAFGGQKKELKRYQKNLEDAKNVGIRKAIMVNISVGFVYFMIYMSYALAFWYGSTLIIAKEYQVGNLLTVFFAVIIGAFGIGQTSPNIQAFASARGAAHKVFSIIDHKPSIDSFSEEGYKPDV--VKGNIEFRNIHFNYPSRQDVKVLNGMNLKIRSGQTIALVGSSGCGKSTTVQLLQRFYDPQEGTVTIDDHDIRSMNVRCLRELIGVVSQEPVLFATSIAENIRYGRED--VTQEEIEQATREANAYDFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAIVQAALDKVRLGRTTIIVAHRLSTIRNADVIAGFQNGKIVELGTHDELMGMKGVYQSLVTMQEEK 425
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Cavefish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414]) HSP 1 Score: 521.546 bits (1342), Expect = 2.723e-156 Identity = 283/609 (46.47%), Postives = 381/609 (62.56%), Query Frame = 1
Query: 349 MICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTL--------MSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASL-IREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
M+ ++ S G P++ +VFG+ T L+ Q T N +N T SS+ T M+ + F GI + ++ V FVITS Q IR +F AV+ Q + WFD L +L DDINT++ G+GDKI +Q++ F+ G I+ F+ GWKLTLV +S+SPL+ + SK + LT + AY KAG ++ E+LG+IRT+V+F G + + +Y NL EA G+RK+I T +G+ L+F ++AL FWYG L + E + +G +L VFFS ++ +GQ P++ S +AR AA I+ ID ID + +G K + G I FK + F+YP+R D IL G+SL + G+T+ALVGASGCGKS+ IQL+QRFYD + GEV +D +I+ LNV WLRE +G+VSQEPVLFG +I ENIRYGR D TD++ID+A+ ANA FI+ LP+ L+T+VG+RG QLSGGQKQRIAIARALV+NPKILLLDEATSALD++SE IVQ ALDK GRTTI++AHRLST+R A VI G KDG+V EQGTH ELM G+Y LV +Q
Sbjct: 1 MLVGLLCSAIHGAALPIMFIVFGSMTDSF------------ILSGQQLNFTG------------NYTFNLTKRSSDCMTFPGEDIEGSMTKNAYYFVGIGAGVLLLGTFQVMLFVITSTRQTKRIREKYFHAVLHQPMAWFDTHPIRELNTRLTDDINTINDGLGDKICIFVQYFCRFLAGFIIGFIYGWKLTLVIMSVSPLLAGSAALCSKILATLTGRELSAYAKAGSVSAEILGAIRTVVAFNGRQKALEKYETNLLEAKNFGVRKAITTNAAMGITQFLLFSTFALAFWYGTKLSVDEPENYSIGKVLTVFFSVMIGALSLGQGAPNLESIAKARGAAYSIYSTIDMPRPIDSSSIKGYKPDSVSGDIEFKKIHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDAGEVTLDGRDIRCLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSEAIVQAALDKARAGRTTIVIAHRLSTIRTADVIAGFKDGEVVEQGTHRELMNKKGVYYSLVMQQ 585 HSP 2 Score: 489.574 bits (1259), Expect = 2.826e-145 Identity = 246/584 (42.12%), Postives = 381/584 (65.24%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA--NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ-SH 4048
F +++ LN PE + +G +++ G V P ILF ++ A +P +++Q + + + + L G +AF++ Q G SGE LT R RS F+A+L+QDI+WFD+ N +G LTT+L+ +A+ ++G +G L ++ CS+G +V ++ F WQ++L++LA VP + +Q R M+G ++ D+ ALE SGKI+ E +EN RTV LT+E F+Q + +++ + KA G + L QA+ + AA F +L+ + ++ ++++ F L+ MN+G++ + +F K K AA + LL++ P I+ +S GV N GDI+ +V FSYP +P++ IL ++ ++ QT+ALVG SGCGKST I LL+RFY+ G + D + +N+ LR +VSQEP LF +I ENI YGD++++ + ++++ AAK +NIH+FI+NLP++Y T+VG+KG+ SGGQKQRIAIARAL+RNP++LLLDEATSALDTESEKIVQ ALD+A++GRTC+VIAHRL+T+QNAD I V ++G++ E G H EL++K G Y +++ Q SH
Sbjct: 667 FGRILALNKPEWPCVLMGTLASCVGGGVYPCVGILFAKIIGVFAELDPEVKRQKSMMFSLLFFLVG--TMAFLTYFFQGYMLGKSGESLTMRLRSQAFKAILRQDISWFDDYDNAVGVLTTKLATDASLVKGAAGSRLSLATSSFCSLGVSVIVALCFSWQLTLLILACVPFLTGAMFIQTRSMKGHASKDQRALETSGKISTETVENFRTVVALTKEEVFMQKFSDSLSEPYKSALWKAPLYGMSFALAQASSYFVRAAFFRFGAWLIAHCYAQYEDIYLVFSVLVLSTMNVGQTSSANLDFAKAKAAAGRIIALLEKKPEIDIYSDAGV--KPTNFRGDIEFHDVHFSYPTQPDVRILQGLNVSVRQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRGLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVVEAAKKANIHEFILNLPERYNTRVGDKGTQLSGGQKQRIAIARALVRNPKVLLLDEATSALDTESEKIVQKALDEARKGRTCIVIAHRLTTVQNADIIAVIQNGQVVEQGMHSELMAKQGAYYALVNAQVSH 1246 HSP 3 Score: 379.022 bits (972), Expect = 2.735e-108 Identity = 211/488 (43.24%), Postives = 308/488 (63.11%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+R+ F+A++RQ+I WFD ++ G LT KL D + V G ++ + +I+A W+LTL+ L+ P + M + +++M K A +G I+ E + + RT+V+ E+ + +++++L E K + K+ L G L + A F +GA LI D+ +VF +++T +GQ + F +A++AA I ++++ PEID ++ G K +F+G I F V F+YPT+ D IL GL++S++ GQT+ALVG SGCGKS+ IQL++RFY+ G+V D + + LN+ WLR Q+G+VSQEP+LF C+I ENI+YG V+ EE+ +A AN FI LP + +T VGD+GTQLSGGQKQRIAIARALVRNPK+LLLDEATSALD+ESEKIVQ ALD+ +GRT I++AHRL+TV+NA +I I++G+V EQG H+ELMA G Y LV+ Q
Sbjct: 757 LRSQAFKAILRQDISWFDDYDNAVGVLTTKLATDASLVKGAAGSRLSLATSSFCSLGVSVIVALCFSWQLTLLILACVPFLTGAMFIQTRSMKGHASKDQRALETSGKISTETVENFRTVVALTKEEVFMQKFSDSLSEPYKSALWKAPLYGMSFALAQASSYFVRAAFFRFGAWLIAHCYA-QYEDIYLVFSVLVLSTMNVGQTSSANLDFAKAKAAAGRIIALLEKKPEIDIYSDAGVKPTNFRGDIEFHDVHFSYPTQPDVRILQGLNVSVRQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRGLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVVEAAKKANIHEFILNLPERYNTRVGDKGTQLSGGQKQRIAIARALVRNPKVLLLDEATSALDTESEKIVQKALDEARKGRTCIVIAHRLTTVQNADIIAVIQNGQVVEQGMHSELMAKQGAYYALVNAQ 1243 HSP 4 Score: 342.813 bits (878), Expect = 2.880e-96 Identity = 219/595 (36.81%), Postives = 325/595 (54.62%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFG-----FLLPAMA-------NPNIEQQSADIRTY------------VIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL-----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+ +G + + +GA P I+FG F+L N+ ++S+D T+ GI + Q + F + + TKR R F A+L Q +AWFD ++ I L TRL+D+ I G+ + + C A I F + W+++LV+++ P++ L +I+ + + A ++G ++ E + IRTV ++ Y+ + + +KA G+TQ +F +A F L + Y++ K+ VF S ++ GA++LG+ + K + AA ++ +D PI+S S G D ++SGDI+ + + FSYP R ++ IL +S + +TIALVG SGCGKST I LLQRFYD G + +D I+ +N++ LR+ IVSQEP LF +I ENI YG TD I A + +N ++FI LPD T VGE+G+ SGGQKQRIAIARAL++NP+ILLLDEATSALDT+SE IVQ ALDKA+ GRT +VIAHRLSTI+ AD I FKDG++ E GTH EL++K G+Y ++ Q+
Sbjct: 1 MLVGLLCSAIHGAALPIMFIVFGSMTDSFILSGQQLNFTGNYTFNLTKRSSDCMTFPGEDIEGSMTKNAYYFVGIGAGVLLLGTFQVMLFVITSTRQTKRIREKYFHAVLHQPMAWFD--THPIRELNTRLTDDINTINDGLGDKICIFVQYFCRFLAGFIIGFIYGWKLTLVIMSVSPLLAGSAALCSKILATLTGRELSAYAKAGSVSAEILGAIRTVVAFNGRQKALEKYETNLLEAKNFGVRKAITTNAAMGITQFLLFSTFALAFWYGTKLSVDEPENYSIGKVLTVFFS---VMIGALSLGQGAPNLESIAKARGAAYSIYSTIDMPRPIDSSSIKGYKPD--SVSGDIEFKKIHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDAGEVTLDGRDIRCLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDA--TDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSEAIVQAALDKARAGRTTIVIAHRLSTIRTADVIAGFKDGEVVEQGTHRELMNKKGVYYSLVMQQT 586
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Cavefish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414]) HSP 1 Score: 517.694 bits (1332), Expect = 9.057e-156 Identity = 259/584 (44.35%), Postives = 390/584 (66.78%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMA--NPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ-SH 4048
F +++ LN PE Y+ +G ++++ GAV P ILF ++ A +P +++Q + + + L G VAF++ Q FG SGE LT R RS F+A+L+QDI+WFD+ +N +G LTT+L+ +A+ ++GV+G +G +++C++G +V I+F F WQ++L++LA VP + +Q+R MQG ++ D+ ALE SGKI+ E +EN +TV LT+E F + + +++ + KA G + L QA + AA F +L+ + ++ NVF+ F +IF AM++G+S + AP+F K K+AA + LL++ P I+ +S G N GDI+ + FSYP RPNI IL ++ ++ QT+ALVG SGCGKST I LL+RFY+ G + D + +N+ LR +VSQEP LF +I ENI YGD++++ + +++ AAK +NIH+FI+NLP++Y T+VG+KG+ SGGQKQRIAIARAL+RNP++LLLDEATSALDTESEKIVQ ALD A++GRTC+VIAHRL+T+QNAD I V ++G++ E G H EL++K+G Y +++ Q SH
Sbjct: 582 FGRILALNKPEWPYVMVGTLASLVGGAVYPCVGILFAKIIGVFAETDPEVKRQKTMMFSLLFLLVG--AVAFITYFFQGYMFGKSGEILTMRLRSQAFKAILRQDISWFDDHNNAVGVLTTKLATDASLVKGVTGSRIGLATSSVCALGISVIIAFCFSWQLTLLILACVPFLTGANFIQMRAMQGHASKDQSALETSGKISTETVENFKTVVALTKEDVFFRKFNDSLAEPYKSALCKAPIYGITFALAQAIPYLVNAAVFRFGAWLIAHCHTQYENVFLVFSVIIFAAMSIGQSSSFAPDFAKAKVAAGRILSLLEKTPEIDIYSEAGD--KPMNFKGDIEFHELHFSYPTRPNIKILQGLNVSVRQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRMLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVEEAAKNANIHEFILNLPERYNTRVGDKGTQLSGGQKQRIAIARALVRNPKVLLLDEATSALDTESEKIVQKALDDARQGRTCIVIAHRLTTVQNADIIAVIQNGQVVEQGMHSELMAKHGAYYALVNAQVSH 1161 HSP 2 Score: 505.368 bits (1300), Expect = 1.378e-151 Identity = 258/517 (49.90%), Postives = 353/517 (68.28%), Query Frame = 1
Query: 601 SQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASL-IREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
S F GI + ++ V F++T+ Q IR +F AV+ Q + WFD G L +L DDINT++ G+GDKI +Q++ F+ GII+ F+ GWKLTLV +S+SPL+ + SK + LT K AY KAG +AEE+L +IRT+V+F G++ + +Y +NL++A G++K+I T +G+ +FG+YAL FWYG L + E + +G ++ VFFS ++ +GQ P++ S +AR AA I+ ID ID ++EG K + +G I FK + F+YP+R D IL G+SL + G+T+ALVGASGCGKS+ IQL+QRFYD + GEV +D +I+ LNV WLRE +G+VSQEPVLFG +I ENIRYGR D TD++ID+A+ ANA FI+ LP+ L+T+VG+RG QLSGGQKQRIAIARALV+NPKILLLDEATSALD++SE Q GRTTI++AHRLST+R A VI G K+G+V EQGTH EL+ G+Y LV +Q
Sbjct: 22 SYYFVGIGAGVLLLGTFQVMLFLLTATRQTKRIREKYFHAVLHQQMAWFDTHPIGELNTRLTDDINTINDGLGDKICIFVQFFCRFLAGIIIGFIYGWKLTLVIMSVSPLLAGSAAVWSKILATLTGKELSAYAKAGAVAEEILVAIRTVVAFNGQQKALEKYEKNLEDAKNFGVKKAITTNVSMGITQFFIFGTYALAFWYGTKLSVDEPENYSIGKVITVFFSVMIGAFSLGQGAPNLESVAKARGAAYSIYNTIDMPRPIDSSSKEGHKPDSVRGDIEFKNIHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDAGEVTLDGRDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSES--QGNFPNARAGRTTIVIAHRLSTIRTADVIAGFKEGEVVEQGTHRELLNKKGVYYSLVMQQ 536 HSP 3 Score: 387.111 bits (993), Expect = 1.445e-111 Identity = 215/489 (43.97%), Postives = 309/489 (63.19%), Query Frame = 1
Query: 697 IRNAFFRAVMRQNIGWFDAQNS--GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIG-GIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+R+ F+A++RQ+I WFD N+ G LT KL D + V G +IG + C +G +I+AF W+LTL+ L+ P + + + M K A +G I+ E + + +T+V+ E ++ ++L E K + K+ + G L + + A F +GA LI + ++ +VF + A IGQ++ F +A+ AA I ++++ PEID +E GDK +F+G I F + F+YPTR + IL GL++S++ GQT+ALVG SGCGKS+ IQL++RFY+ G+V D + + LN+ WLR Q+G+VSQEP+LF C+I ENI+YG V+ EE+++A AN FI LP + +T VGD+GTQLSGGQKQRIAIARALVRNPK+LLLDEATSALD+ESEKIVQ ALD +GRT I++AHRL+TV+NA +I I++G+V EQG H+ELMA +G Y LV+ Q
Sbjct: 672 LRSQAFKAILRQDISWFDDHNNAVGVLTTKLATDASLVKGVTGSRIG-LATSSVCALGISVIIAFCFSWQLTLLILACVPFLTGANFIQMRAMQGHASKDQSALETSGKISTETVENFKTVVALTKEDVFFRKFNDSLAEPYKSALCKAPIYGITFALAQAIPYLVNAAVFRFGAWLIAHCHT-QYENVFLVFSVIIFAAMSIGQSSSFAPDFAKAKVAAGRILSLLEKTPEIDIYSEAGDKPMNFKGDIEFHELHFSYPTRPNIKILQGLNVSVRQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRMLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVEEAAKNANIHEFILNLPERYNTRVGDKGTQLSGGQKQRIAIARALVRNPKVLLLDEATSALDTESEKIVQKALDDARQGRTCIVIAHRLTTVQNADIIAVIQNGQVVEQGMHSELMAKHGAYYALVNAQ 1158 HSP 4 Score: 332.028 bits (850), Expect = 2.574e-93 Identity = 204/521 (39.16%), Postives = 297/521 (57.01%), Query Frame = 2
Query: 2495 GIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL-----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
GI + Q + F + + TKR R F A+L Q +AWFD ++ IG L TRL+D+ I G+ + + C A + I F + W+++LV+++ P++ + +I+ + + A ++G +A E + IRTV + ++ Y++ ++ KKA G+TQ IF YA F L + Y++ K+ VF S ++ GA +LG+ + K + AA ++ +D PI+S S +G D ++ GDI+ +N+ FSYP R ++ IL +S + +TIALVG SGCGKST I LLQRFYD G + +D I+ +N++ LR+ IVSQEP LF +I ENI YG TD I A + +N ++FI LPD T VGE+G+ SGGQKQRIAIARAL++NP+ILLLDEATSALDT+SE Q A+ GRT +VIAHRLSTI+ AD I FK+G++ E GTH ELL+K G+Y ++ Q
Sbjct: 27 GIGAGVLLLGTFQVMLFLLTATRQTKRIREKYFHAVLHQQMAWFD--THPIGELNTRLTDDINTINDGLGDKICIFVQFFCRFLAGIIIGFIYGWKLTLVIMSVSPLLAGSAAVWSKILATLTGKELSAYAKAGAVAEEILVAIRTVVAFNGQQKALEKYEKNLEDAKNFGVKKAITTNVSMGITQFFIFGTYALAFWYGTKLSVDEPENYSIGKVITVFFS---VMIGAFSLGQGAPNLESVAKARGAAYSIYNTIDMPRPIDSSSKEGHKPD--SVRGDIEFKNIHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDAGEVTLDGRDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDA--TDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSES--QGNFPNARAGRTTIVIAHRLSTIRTADVIAGFKEGEVVEQGTHRELLNKKGVYYSLVMQQ 536
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Sea Lamprey
Match: ABCB1 (ATP binding cassette subfamily B member 1 [Source:HGNC Symbol;Acc:HGNC:40]) HSP 1 Score: 564.303 bits (1453), Expect = 7.324e-174 Identity = 278/579 (48.01%), Postives = 407/579 (70.29%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
F+KV+ LN E YI LG + A NGA+QP+F+++F ++ A ++ ++ Y + + +V+FV+ LQ FG SGE LT + RS+ AML+QD+ WFD+P N+ GALTTRL+ +A+Q++G +G L + I +MG A+ ISF + W+++L++LA VP + G L+++ + G +N DK LEE+GKIA E +ENIRTV++LTRE T + Y+++++ KK+ G YG +Q ++ A AA F +L+ + NVF+ F A++FGAM +G++ + AP++ K K +A H+F L +P I+S+S +G L E+ G+++ ++V F+YP+RPN+ +L +S + + QT+ALVG SGCGKST + LL+RFYDTL+GN+LID ++ ++IQ LR IVSQEP LF S+ ENIAYGD+++I D+I+AAA+A+NIH FI +LP++Y TKVG+KG+ SGGQKQRIAIARAL+R P+ILLLDEATSALDTESEKIVQ+ALD+A+EGRTC+VIAHRLST+Q ADKI V ++G++ E GTH +LL+K G Y +++ Q
Sbjct: 481 FWKVLALNKKEWPYITLGTLCAGVNGALQPAFAVIFSRIIGVFAETDLVKREEQSNLYSLLFLVLGIVSFVTFFLQGFLFGRSGELLTMKLRSMGLRAMLRQDMMWFDDPKNSTGALTTRLATDASQVQGATGARLATIAQNIANMGTAIVISFVYGWELTLLILAIVPFVGAAGFLEMQALTGQANRDKKLLEEAGKIATEAVENIRTVASLTRERTLEKIYQDSLEGPAKTAKKKSQFYGLTYGFSQGVVYLAQAAAFRFGAFLVTAGRMEFENVFLVFSAIVFGAMAVGQTSSFAPDYAKAKTSANHLFYLFQLIPAIDSYSDEGEKL--EDCEGNVEFQSVGFNYPMRPNVEVLKGLSLKVENGQTLALVGSSGCGKSTSVQLLERFYDTLSGNVLIDGKDVRSLHIQWLRAQMGIVSQEPVLFDCSMAENIAYGDNSRIVPQDEIVAAARAANIHTFIDSLPEKYNTKVGDKGTQLSGGQKQRIAIARALVRQPKILLLDEATSALDTESEKIVQEALDRAREGRTCIVIAHRLSTVQTADKIAVIQNGQVIEQGTHQQLLAKKGAYHLLVNAQ 1057 HSP 2 Score: 405.601 bits (1041), Expect = 2.855e-119 Identity = 214/416 (51.44%), Postives = 290/416 (69.71%), Query Frame = 1
Query: 901 TLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
TLV L+ISP++ I + SK + T + AY KAG +AEEVL +IRT+++F G++ E+ RY +NL EA IGI+K+I T +G M +++ SY+L FWYG+ L+ E + +G+++IVFF+ ++ GIGQA P + + + AR AA IFEIIDR + N L + + + T+ + C +L G++L ++ GQTVALVG+SGCGKS+ IQL+QRFYD +G +++D +++ LN+ LR+ IGVVSQEPVLF +I ENIR+G VT E+I++A ANA FI+ +P + +TLVGDRG QLSGGQKQRIAIARAL+RNPK+LLLDEATSALD ESE IVQ+ALDK EGRTTI++AHRLST+RNA +I G G + E GTH ELM NG+Y LV Q
Sbjct: 1 TLVILAISPVLAISAGLWSKILTSFTNRELLAYSKAGAVAEEVLSAIRTVMAFGGQEKELKRYDKNLVEARNIGIKKAITTNLSIGFTMFIVYASYSLAFWYGSRLVLEDTSYTIGNVMIVFFAVIIGAFGIGQAAPFLEAISVARGAAYTIFEIIDRTIHMYPFNTHNSSLRN-RKRCTWFREHTLVNSIAFCQVLKGVNLQVKSGQTVALVGSSGCGKSTTIQLLQRFYDPQQGTIMVDGRDVRTLNLRSLRQLIGVVSQEPVLFATTIAENIRFGHEKVTMEDIERAARNANAYDFISQMPERFNTLVGDRGAQLSGGQKQRIAIARALIRNPKLLLLDEATSALDGESEAIVQEALDKAREGRTTIVIAHRLSTIRNADIIAGFDGGVIVELGTHDELMKRNGVYHTLVSMQ 415 HSP 3 Score: 397.512 bits (1020), Expect = 1.573e-116 Identity = 220/530 (41.51%), Postives = 328/530 (61.89%), Query Frame = 1
Query: 571 SNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
SN+++L+ F + + F+ ++ F + + +R+ RA++RQ++ WFD ++GALT +L D + V G ++ + Q A I+++FV GW+LTL+ L+I P + + + + + + +AG IA E + +IRT+ S E+ Y ++L+ K +KS G G G+++ + A F +GA L+ G ++ +VF + + +GQ + + +A+++A +F + +P ID ++EG+KL D +G + F+ V F YP R + +L GLSL ++ GQT+ALVG+SGCGKS+ +QL++RFYD+ G VLID +++ L++ WLR Q+G+VSQEPVLF CS+ ENI YG V +EI A AN TFI++LP K +T VGD+GTQLSGGQKQRIAIARALVR PKILLLDEATSALD+ESEKIVQ+ALD+ EGRT I++AHRLSTV+ A I I++G+V EQGTH +L+A G Y LV+ Q
Sbjct: 535 SNLYSLL------FLVLGIVSFVTFFLQGFLFGRSGELLTMKLRSMGLRAMLRQDMMWFDDPKNSTGALTTRLATDASQVQGATGARLATIAQNIANMGTAIVISFVYGWELTLLILAIVPFVGAAGFLEMQALTGQANRDKKLLEEAGKIATEAVENIRTVASLTRERTLEKIYQDSLEGPAKTAKKKSQFYGLTYGFSQGVVYLAQAAAFRFGAFLVTAGR-MEFENVFLVFSAIVFGAMAVGQTSSFAPDYAKAKTSANHLFYLFQLIPAIDSYSDEGEKLEDCEGNVEFQSVGFNYPMRPNVEVLKGLSLKVENGQTLALVGSSGCGKSTSVQLLERFYDTLSGNVLIDGKDVRSLHIQWLRAQMGIVSQEPVLFDCSMAENIAYGDNSRIVPQDEIVAAARAANIHTFIDSLPEKYNTKVGDKGTQLSGGQKQRIAIARALVRQPKILLLDEATSALDTESEKIVQEALDRAREGRTCIVIAHRLSTVQTADKIAVIQNGQVIEQGTHQQLLAKKGAYHLLVNAQ 1057 HSP 4 Score: 293.893 bits (751), Expect = 4.490e-82 Identity = 178/429 (41.49%), Postives = 259/429 (60.37%), Query Frame = 2
Query: 2786 SLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMS-KMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIII--------LNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+LV+LA P++ + L +I+ F+N + A ++G +A E + IRTV + ++ Y + + + KKA G T ++ +Y+ F L+L + S + NV I F A+I GA +G++ + AA +FE++DR + F+T N S + N K R + ++ L V+ + S QT+ALVG SGCGKST I LLQRFYD G I++D ++ +N+++LRQL +VSQEP LF+ +I ENI +G K+ +D I AA+ +N +DFI +P+++ T VG++G+ SGGQKQRIAIARAL+RNP++LLLDEATSALD ESE IVQ+ALDKA+EGRT +VIAHRLSTI+NAD I F G I E GTH EL+ +NG+Y ++S+Q+
Sbjct: 1 TLVILAISPVLAISAGLWSKILTSFTNRELLAYSKAGAVAEEVLSAIRTVMAFGGQEKELKRYDKNLVEARNIGIKKAITTNLSIGFTMFIVYASYSLAFWYGSRLVLEDTSYTIGNVMIVFFAVIIGAFGIGQAAPFLEAISVARGAAYTIFEIIDRTIHMYPFNT-------HNSS----LRNRKRCTWFREHTLVNSIAFCQVLKGVNLQVKSGQTVALVGSSGCGKSTTIQLLQRFYDPQQGTIMVDGRDVRTLNLRSLRQLIGVVSQEPVLFATTIAENIRFGHE-KVTMED-IERAARNANAYDFISQMPERFNTLVGDRGAQLSGGQKQRIAIARALIRNPKLLLLDEATSALDGESEAIVQEALDKAREGRTTIVIAHRLSTIRNADIIAGFDGGVIVELGTHDELMKRNGVYHTLVSMQT 416
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Sea Lamprey
Match: abcb11b (ATP-binding cassette, sub-family B (MDR/TAP), member 11b [Source:ZFIN;Acc:ZDB-GENE-050517-14]) HSP 1 Score: 476.093 bits (1224), Expect = 6.433e-141 Identity = 270/619 (43.62%), Postives = 383/619 (61.87%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSL---TQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G+ +LF +A DIV+M+ + +I G P ++LVFG Y L + + + + T+IP+ ++I ++ + + GI + ++ Y+ + F+V+ + Q+ IR AFF +MR IGWFD + G L ++ DDIN ++ I DK+ +Q + F+ G ++ F+ GWKLTLV L++SPL+ + ++ + ++T + +AY KAG +AEEVL SIRT+ +F GEK E RY +NL A K GIRK ++ G G + ++F YAL FWYG++L+ E + G +L VFF L+ +GQA+P + +F R AA +F IIDR P ID M+++G+ L +G I F VKF+YPTR D L +L + G+T A VG SG GKS+ +QL+QRFYD EG+V +D +I+ LN+ W+R IGVV QEP+LF +I ENIRYGR +VT E++QA ANA FI LP+K DT+VG+ G Q+SGGQKQRIAIARALVRNP+ILLLD+ATSALD+ESE +VQ ALDKV +G TTI +AHRLST+ +A VI G + G E+G+H LMA G+Y LV Q
Sbjct: 52 GLCQLFRFAKGGDIVLMVTGALGAIVHGAALPFMLLVFGRMADAFIEYDLETQELQDPNKMCLNNSILWVNGTTIPNAT----------ACGHTDIEVELTTFAFYYLGIGCVVLLVGYLQICFWVMAAARQVLRIRQAFFHNIMRMEIGWFDMNSVGELNTRISDDINKINDAIADKMSIFIQRFTTFVCGFLIGFLNGWKLTLVILAVSPLLGLGGALMGSFVARMTGRELKAYAKAGAVAEEVLSSIRTVAAFGGEKKEENRYDKNLVMAQKWGIRKGMILGFFQGYMWFIIFLCYALAFWYGSNLVLEEEEYSPGTLLQVFFGVLVGAINLGQASPCLEAFASGRGAATHVFNIIDREPIIDCMSDDGNILQKVKGDIEFHNVKFSYPTRLDLTALHSFNLKVNAGETTAFVGPSGAGKSTAMQLIQRFYDPQEGKVTLDGHDIRSLNLRWMRSLIGVVEQEPILFATTIAENIRYGRDNVTVSEVEQAARRANAYNFIMELPHKFDTMVGEGGGQMSGGQKQRIAIARALVRNPRILLLDQATSALDNESEAVVQSALDKVRKGCTTISIAHRLSTIHSADVIVGFELGCAVERGSHEALMARQGVYFTLVTLQ 660 HSP 2 Score: 448.358 bits (1152), Expect = 1.452e-131 Identity = 244/575 (42.43%), Postives = 348/575 (60.52%), Query Frame = 2
Query: 2312 KVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMIS 4036
+++ NAPE Y+ G V A NG +QP F+ILF +L P E+Q I + I +V+FV+ LQ F SGE LT+R R F AML+QD+ WFD+ N+ GALTTRL+ +A+Q++GV + + + ++ W+ ++ + IR G N + A E I NIRTV+ L +E TF++ Y +++ +KA+ G + +Q+ IF YAA+F G+L+ ++ K VF F AL+F LG++ + AP++ K KI+AA +F+L DR P I+S GV D GD+ N F+YP RP+ +LN +S ++ QT+ALVG SGCGKST + LL+RFYD G +LID + +N+ LR IVSQEP LF SI +NI YG + + T D++I A+KA+ +HDF++ LP++YET VG+KG+ S G+KQR+AIARA++R+PRILLLDEATSALDTESE+ VQ ALD+A++GRTC+VIAHRLSTIQNAD+I V G + E GTH EL+ G Y ++++
Sbjct: 742 RILKYNAPEWPYMVFGAVGAAINGGIQPVFAILFSEILGTFTLPTEEEQRNMINLTCLLFVLIGIVSFVTYFLQGYMFSKSGELLTRRLRRCGFTAMLRQDVGWFDDLKNSPGALTTRLATDASQVQGVKICYVFEFHSRMFFSFSSSCCCCSHRWERQTFIMN----------IYIRTPYGEDNRTRNA---RAHFVNESIVNIRTVAGLGKERTFVKQYTDSLTVPHRAAIRKANVFGATFSFSQSVIFFTYAASFRYGGFLVAHDGLKFPLVFRVFSALVFSGTALGRASSYAPDYAKAKISAARLFKLFDRTPAIDSSPDKGVSWD--GFRGDVTFTNCTFTYPSRPDTQVLNGLSVSVQPGQTLALVGTSGCGKSTSVQLLERFYDPDGGQVLIDGRDSRTVNVGFLRSKIGIVSQEPVLFDCSIADNIRYGANFRELTWDEVINASKAAQLHDFVMGLPEKYETNVGDKGAQLSRGEKQRVAIARAIIRDPRILLLDEATSALDTESEQTVQSALDQARQGRTCIVIAHRLSTIQNADQIAVISQGLVIEKGTHNELMDAQGAYYKLVT 1301 HSP 3 Score: 339.732 bits (870), Expect = 1.095e-95 Identity = 199/517 (38.49%), Postives = 300/517 (58.03%), Query Frame = 1
Query: 610 FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYA---CFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGR--MDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
F I + F+ ++ F + + +R F A++RQ++GWFD + GALT +L D + V G KI + ++++ W+ ++I + +A + E ++ +IRT+ E+ V +YT++L + IRK+ + G ++F +YA F YG L+ +G + VF + + + T +G+A+ + + +A+ +AA +F++ DR P ID ++G + F+G +TF FTYP+R D +L GLS+S+QPGQT+ALVG SGCGKS+ +QL++RFYD + G+VLID + + +NV +LR +IG+VSQEPVLF CSI +NIRYG ++T +E+ A A F+ LP K +T VGD+G QLS G+KQR+AIARA++R+P+ILLLDEATSALD+ESE+ VQ ALD+ +GRT I++AHRLST++NA I I G V E+GTH ELM G Y LV
Sbjct: 801 FVLIGIVSFVTYFLQGYMFSKSGELLTRRLRRCGFTAMLRQDVGWFDDLKNSPGALTTRLATDASQVQ---GVKICYVFEFHSRMFFSFSSSCCCCSHRWERQTFIMNI------------YIRTPYGEDNRTRNARAHFVNESIV-NIRTVAGLGKERTFVKQYTDSLTVPHRAAIRKANVFGATFSFSQSVIFFTYAASFRYGGFLVAH-DGLKFPLVFRVFSALVFSGTALGRASSYAPDYAKAKISAARLFKLFDRTPAIDSSPDKGVSWDGFRGDVTFTNCTFTYPSRPDTQVLNGLSVSVQPGQTLALVGTSGCGKSTSVQLLERFYDPDGGQVLIDGRDSRTVNVGFLRSKIGIVSQEPVLFDCSIADNIRYGANFRELTWDEVINASKAAQLHDFVMGLPEKYETNVGDKGAQLSRGEKQRVAIARAIIRDPRILLLDEATSALDTESEQTVQSALDQARQGRTCIVIAHRLSTIQNADQIAVISQGLVIEKGTHNELMDAQGAYYKLV 1300 HSP 4 Score: 307.76 bits (787), Expect = 2.188e-85 Identity = 213/601 (35.44%), Postives = 321/601 (53.41%), Query Frame = 2
Query: 2357 GCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQS---------------------------------ADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
G + AI +GA P ++FG + A ++E Q ++ T+ GI V + LQ + + + R R F +++ +I WFD N++G L TR+SD+ +I + + I + I F W+++LV+LA P++ +GG L + + + A ++G +A E + +IRTV+ E Y + + +K GF G IF YA F L+L Y+ + VF FG L+ GA+NLG++ F G+ AA H+F ++DR P I+ S DG +L + + GDI+ NVKFSYP R ++ L+ + +N+ +T A VGPSG GKST + L+QRFYD G + +D + I+ +N++ +R L +V QEP LF+ +I ENI YG T ++ AA+ +N ++FI+ LP +++T VGE G SGGQKQRIAIARAL+RNPRILLLD+ATSALD ESE +VQ ALDK ++G T + IAHRLSTI +AD I+ F+ G E G+H L+++ G+Y +++LQS
Sbjct: 71 GALGAIVHGAALPFMLLVFGRMADAFIEYDLETQELQDPNKMCLNNSILWVNGTTIPNATACGHTDIEVELTTFAFYYLGIGCVVLLVGYLQICFWVMAAARQVLRIRQAFFHNIMRMEIGWFD--MNSVGELNTRISDDINKINDAIADKMSIFIQRFTTFVCGFLIGFLNGWKLTLVILAVSPLLGLGGALMGSFVARMTGRELKAYAKAGAVAEEVLSSIRTVAAFGGEKKEENRYDKNLVMAQKWGIRKGMILGFFQGYMWFIIFLCYALAFWYGSNLVLEEEEYSPGTLLQVF--FGVLV-GAINLGQASPCLEAFASGRGAATHVFNIIDREPIIDCMSDDGNIL--QKVKGDIEFHNVKFSYPTRLDLTALHSFNLKVNAGETTAFVGPSGAGKSTAMQLIQRFYDPQEGKVTLDGHDIRSLNLRWMRSLIGVVEQEPILFATTIAENIRYGRDN--VTVSEVEQAARRANAYNFIMELPHKFDTMVGEGGGQMSGGQKQRIAIARALVRNPRILLLDQATSALDNESEAVVQSALDKVRKGCTTISIAHRLSTIHSADVIVGFELGCAVERGSHEALMARQGVYFTLVTLQSQ 662
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001786.1 (pep scaffold:Pmarinus_7.0:GL477870:201625:214977:-1 gene:ENSPMAG00000001622.1 transcript:ENSPMAT00000001786.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 433.335 bits (1113), Expect = 1.162e-135 Identity = 215/478 (44.98%), Postives = 323/478 (67.57%), Query Frame = 2
Query: 2609 QDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
Q+I WF NN GALT +LS +A Q++G +G G ++ + + + I+ W+++L++LAF+P + G++Q + + + K A E++ KI+ E ++NIRTV++L E F +++ + + + ++ + +G TQ + AYAA F L G+L+ +V+ FGA+IFGA+ +G++MAMAPN+G+ K++AA + LL R I+S+S G+ D G++++ V F YP RP+ +L VS + QT+ALVGPSG GKSTI+ LL RFYD+ G +L++ +++ +N+ LR+ +VSQEP LF SI ENIAYGD ++ + D+++AAA+A+N+HDF+ +LP Y+T VGE+G+ SGGQKQR+AIARALLR+PR+LLLDEATSALD ESEK+VQDAL+KAQ+GRTC+VIAHRLST++ AD I V +DG I E G+H ELLS G+Y +++ Q
Sbjct: 7 QEIRWFYNHKNNTGALTAQLSTDAAQVQGATGRRAGMLVQNVSGLATGLLIALLVGWKLALLMLAFMPFMVGAGLVQSVLQGSEATTIKRAYEQASKISTEAVQNIRTVASLCLEEKFSGMFEQCLLEPYRVIKRRGYLFSVTFGFTQGILCFAYAAVFRLGGFLVAQGELTFQDVYKVFGAIIFGALAVGQAMAMAPNYGRAKVSAARILALLRREMAIDSYSEGGIQPDA--FDGELQLSGVHFRYPTRPHAEVLRGVSLRVAPGQTLALVGPSGSGKSTIMQLLLRFYDSSAGQLLLNGVEVRQLNLGWLRRQLGVVSQEPVLFGCSIAENIAYGDVSRAVSRDEVVAAARAANLHDFVESLPQAYDTVVGERGAQLSGGQKQRVAIARALLRDPRVLLLDEATSALDAESEKVVQDALEKAQQGRTCIVIAHRLSTVRAADVIAVLQDGDICELGSHEELLSARGLYYRLVEAQ 482 HSP 2 Score: 364.77 bits (935), Expect = 6.788e-112 Identity = 202/477 (42.35%), Postives = 291/477 (61.01%), Query Frame = 1
Query: 730 QNIGWF--DAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
Q I WF N+GALT +L D V G + G ++Q + G+++A + GWKL L+ L+ P ++ L+ S AY +A I+ E + +IRT+ S E+ + + L E ++ R+ L G G++ +YA F G L+ +G D+ VF + + +GQA ++ A+ +AA I ++ R ID +E G + + F G++ V F YPTR +L G+SL + PGQT+ALVG SG GKS+++QL+ RFYDS+ G++L++ ++ LN+ WLR Q+GVVSQEPVLFGCSI ENI YG + V+ +E+ A AN F+ +LP DT+VG+RG QLSGGQKQR+AIARAL+R+P++LLLDEATSALD+ESEK+VQDAL+K +GRT I++AHRLSTVR A VI ++DG + E G+H EL++ GLY LV+ Q
Sbjct: 7 QEIRWFYNHKNNTGALTAQLSTDAAQVQGATGRRAGMLVQNVSGLATGLLIALLVGWKLALLMLAFMPFMVGAGLVQSVLQGSEATTIKRAYEQASKISTEAVQNIRTVASLCLEEKFSGMFEQCLLEPYRVIKRRGYLFSVTFGFTQGILCFAYAAVFRLGGFLVAQGE-LTFQDVYKVFGAIIFGALAVGQAMAMAPNYGRAKVSAARILALLRREMAIDSYSEGGIQPDAFDGELQLSGVHFRYPTRPHAEVLRGVSLRVAPGQTLALVGPSGSGKSTIMQLLLRFYDSSAGQLLLNGVEVRQLNLGWLRRQLGVVSQEPVLFGCSIAENIAYGDVSRAVSRDEVVAAARAANLHDFVESLPQAYDTVVGERGAQLSGGQKQRVAIARALLRDPRVLLLDEATSALDAESEKVVQDALEKAQQGRTCIVIAHRLSTVRAADVIAVLQDGDICELGSHEELLSARGLYYRLVEAQ 482
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Sea Lamprey
Match: abcb10 (ATP-binding cassette, sub-family B (MDR/TAP), member 10 [Source:ZFIN;Acc:ZDB-GENE-040525-2]) HSP 1 Score: 317.775 bits (813), Expect = 3.491e-94 Identity = 188/526 (35.74%), Postives = 301/526 (57.22%), Query Frame = 1
Query: 583 TLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD---VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMA-LNGLYKDLVDRQ 2148
T ++ ++ +GI +A I + V ++ Q + +R++ FRA+MRQ +G+FD +G L N+L D + + + + ++ A GI + F KL L + P I + ++ + + ++T++ ++ +A +AEE +G+IRT+ SF E+ E+ +Y+ ++ ++ ++++ G L Y L+ + VGD+ IG + + + I++++DR P I + L +G+I F+ V F YPTR P+ L L++ GQ A+VG SGCGKS+L+ L+ R YD +G+VLID +++++N WLR QIG VSQEP+LF CSI ENI YG + + +I +A ANA FI P+ DT+VG++G LSGGQKQRIAIARA+++NP ILLLDEATSALDSESE +VQ+ALD+++ GRT +++AHRLST++ A I ++ G+V + GT+A+L A G++ LV+RQ
Sbjct: 69 TDLAHLATVLSGIFMAGAIANAGRVYLMQVSGQRIVQRLRDSLFRAIMRQELGFFDRTRTGELVNRLSADTALMGRAVTENLSDGLRSVAQVTVGIGMMFYVSPKLATFVLGVVPPIALIAVVYGRYLRRITKRTQDSLAEATQVAEERIGNIRTVRSFAKEELEMQKYSSKVNYVLQLAYKEALARAGFFGATGLSGNL-IVLAVLYKGGLLMQSAHITVGDLSAFLLYSGWVGISIGGLSSFYSELMKGLGTGSRIWQLLDRQPTIPLGEGQVVPLQALRGEIEFRGVHFAYPTRETAPVFRSLDLAVPAGQVTAVVGPSGCGKSTLLSLLLRLYDPQKGQVLIDGQDVREVNPSWLRSQIGTVSQEPILFSCSIAENIAYGAANPEAASKADIARAAQAANAYGFIEGFPSGFDTVVGEKGVLLSGGQKQRIAIARAILKNPNILLLDEATSALDSESESLVQEALDRLMTGRTVLVIAHRLSTIQGADRIAVLERGRVAQAGTYAQLTADTGGMFHRLVERQ 593 HSP 2 Score: 269.24 bits (687), Expect = 1.760e-77 Identity = 203/600 (33.83%), Postives = 324/600 (54.00%), Query Frame = 2
Query: 2294 SSAGFFKVMGLNAPEK----CYIFLGCVSAIFNGAVQPSFSILFGFLLPAM-ANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVG----FGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLS-DEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMT----NVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDS-AKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKN-GIYSQMISLQS 4045
SS K+M L PE + L C+S++ V S G ++ + + ++ D+ L+GI F++ + N G SG+++ +R R F A+++Q++ +FD G L RLS D A R V+ E+L + ++ + + + F +++ VL VP I + ++ R ++ + + +L E+ ++A E I NIRTV + +E +Q Y + + K+A A+ +G T + A G LL + +T + F+ + + +++G + KG + +++LLDR P I +G V+ ++ + G+I+ V F+YP R + + + + Q A+VGPSGCGKST++ LL R YD G +LID ++++N LR VSQEP LFS SI ENIAYG + + + I AA+A+N + FI P ++T VGEKG SGGQKQRIAIARA+L+NP ILLLDEATSALD+ESE +VQ+ALD+ GRT +VIAHRLSTIQ AD+I V + G++A++GT+ +L + G++ +++ Q+
Sbjct: 12 SSKDLKKLMALARPESRTLAAAVGLLCISSV----VTMSVPFCLGKVIDVINSTGGVDMAPTDLAHLATVLSGI----FMAGAIANAGRVYLMQVSGQRIVQRLRDSLFRAIMRQELGFFDR--TRTGELVNRLSADTALMGRAVT-ENLSDGLRSVAQVTVGIGMMFYVSPKLATFVLGVVPPIALIAVVYGRYLRRITKRTQDSLAEATQVAEERIGNIRTVRSFAKEELEMQKYSSKVNYVLQLAYKEALARAGFFGATGLSGNLIVLAVLYKGG--LLMQSAHITVGDLSAFLLYSGWV--GISIGGLSSFYSELMKGLGTGSRIWQLLDRQPTIPL--GEGQVVPLQALRGEIEFRGVHFAYPTRETAPVFRSLDLAVPAGQVTAVVGPSGCGKSTLLSLLLRLYDPQKGQVLIDGQDVREVNPSWLRSQIGTVSQEPILFSCSIAENIAYGAANPEAASKADIARAAQAANAYGFIEGFPSGFDTVVGEKGVLLSGGQKQRIAIARAILKNPNILLLDEATSALDSESESLVQEALDRLMTGRTVLVIAHRLSTIQGADRIAVLERGRVAQAGTYAQLTADTGGMFHRLVERQT 594
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Sea Lamprey
Match: abcb8 (ATP-binding cassette, sub-family B (MDR/TAP), member 8 [Source:NCBI gene;Acc:548340]) HSP 1 Score: 308.916 bits (790), Expect = 8.639e-91 Identity = 175/487 (35.93%), Postives = 282/487 (57.91%), Query Frame = 1
Query: 691 SAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQ-IGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+++R + F A++RQ++ +FDA +G L N+L D+ + I Q ++ +G + ++ KLT + + + ++ ++ + KL+++A E KA G+A+E LG++RT+ +F E + +Y +D++CK+ I GL ++ + G SL+ G G+++ + + + G SA A +FE ++ P I + G + F V F+YPTR +L +L++ P QT+A+VG SG GKS++ L++RFYD G + +D +++ L+ WLR + IG ++QEPVLFG +I ENIR+G+ TD+E+ A +ANA FI + P+ +T+VG+RG LSGGQKQR+AIARAL++ P +L+LDEATSALD+E+E VQ+AL++ GRT +++AHRLSTV A++I + G VQE GTHAEL+ GLY +L+ RQ
Sbjct: 135 ASMRKSLFEALIRQDVAFFDANRTGHLVNRLTSDVQEFKSSFKLVISQGLRSVTQTVGCVASLYLISPKLTGLMVVVMTAMVGGGALIGSFLRKLSRRAQEQISKATGVADETLGNVRTVRAFAMEDSKSEQYAVEVDKSCKMNEDLGIGIAIFQGLSNIVLNCIVLGTIFVGGSLM-AGEELSPGELMSFLVASQTVQRSMASVSILFGQMVRGLSAGARLFEYMELEPSIPVRGGGIIPHHSCLGHVRFNNVTFSYPTRPGQVVLHNFNLNLPPSQTIAIVGLSGGGKSTVAALLERFYDPASGSITLDGKDLRTLDPSWLRGRVIGFINQEPVLFGTTIIENIRFGKPGATDDEVYAAARLANADDFIRSFPDGYNTVVGERGATLSGGQKQRVAIARALIKQPAVLVLDEATSALDAEAEGAVQEALERAAHGRTVLVIAHRLSTVAGANLICVVAGGTVQEMGTHAELLKKGGLYAELIRRQ 620 HSP 2 Score: 271.937 bits (694), Expect = 3.634e-78 Identity = 181/514 (35.21%), Postives = 282/514 (54.86%), Query Frame = 2
Query: 2555 GEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRG----VSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGAL---IFGAMNLGKSMA-MAPNFG---KGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLR-QLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMI---SLQSHK 4051
GE++ R FEA+++QD+A+FD +N G L RL+ + + + V + L +V + + + IS + +V+ A V GG L ++ S + + ++ +A E + N+RTV E + + Y + K GL+ + C T + G L M +S G L + + + +SMA ++ FG +G A A +FE ++ P I G ++ + G ++ NV FSYP RP ++L++ + N+ +QTIA+VG SG GKST+ LL+RFYD +G+I +D ++ ++ LR ++ ++QEP LF +I ENI +G TDD++ AAA+ +N DFI + PD Y T VGE+G+ SGGQKQR+AIARAL++ P +L+LDEATSALD E+E VQ+AL++A GRT +VIAHRLST+ A+ I V G + E GTH ELL K G+Y+++I +++ HK
Sbjct: 130 GERIAASMRKSLFEALIRQDVAFFD--ANRTGHLVNRLTSDVQEFKSSFKLVISQGLRSVTQTVGCVASLYLISPKLTGLMVVVMTAMVG----GGALIGSFLRKLSRRAQEQISKATGVADETLGNVRTVRAFAMEDSKSEQYAVEVDKSCKMNEDLGIGIAIFQGLSNIVLNCIVLGTIFVGGSL-------MAGEELSPGELMSFLVASQTVQRSMASVSILFGQMVRGLSAGARLFEYMELEPSIPV--RGGGIIPHHSCLGHVRFNNVTFSYPTRPGQVVLHNFNLNLPPSQTIAIVGLSGGGKSTVAALLERFYDPASGSITLDGKDLRTLDPSWLRGRVIGFINQEPVLFGTTIIENIRFGKPGA--TDDEVYAAARLANADDFIRSFPDGYNTVVGERGATLSGGQKQRVAIARALIKQPAVLVLDEATSALDAEAEGAVQEALERAAHGRTVLVIAHRLSTVAGANLICVVAGGTVQEMGTHAELLKKGGLYAELIRRQNMEQHK 626
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Yeast
Match: MDL1 (Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress [Source:SGD;Acc:S000004178]) HSP 1 Score: 256.914 bits (655), Expect = 1.269e-72 Identity = 189/562 (33.63%), Postives = 295/562 (52.49%), Query Frame = 1
Query: 520 DMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVS----VTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWY-ACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFN----EARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRY---GRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVE-GRTTIIVAHRLSTVRNA-HVIFGIKDGKVQEQGTHAELMA-----LNGLYKDLVDRQ 2148
D E NK Y FT +QF A+FI+ V+ + +T + ++ +R +A + Q+ + D G L ++L D + V + + + F+G +++F+ WKLT V + ++P + L+ + + L+++ + G +AEE L + RTI ++ GEK EV RY + + IG+++++ +G G + + G S+I+ G+ VG++ S +M G + + SF + AAA +FE+ DR P I + D ++ Q I FK V FTYPTR I L+++I+PG+ V VG SG GKS++ L+ R+YD N G + D +I++ N+ R IG V QEP+LF +I +NI Y + D+ I +A+ AN F+ P+ L T+VG RG QLSGGQKQRIA+ARA + +P +L+LDEATSALDS+SE+IV L + VE G TTI +AHRLST++++ VI K G V E G+ +L+A LN L + D +
Sbjct: 138 DEEGSKSNKLYGFT-------------KKQFFTALGAVFIIGAVANASRIIILKVTGERLVARLRTRTMKAALDQDATFLDTNRVGDLISRLSSDASIVAKSVTQNVSDGTRAIIQGFVGFGMMSFL-SWKLTCVMMILAPPLGAMALIYGRKIRNLSRQLQTSVGGLTKVAEEQLNATRTIQAYGGEKNEVRRYAKEVRNVFHIGLKEAVTSGLFFGSTGLVGNTAMLSLLLVGTSMIQSGS-MTVGEL----SSFMMYAVYTGSSLFGLSSFYSELMKGAGAAARVFELNDRKPLI-RPTIGKDPVSLAQKPIVFKNVSFTYPTRPKHQIFKDLNITIKPGEHVCAVGPSGSGKSTIASLLLRYYDVNSGSIEFGDEDIRNFNLRKYRRLIGYVQQEPLLFNGTILDNILYCIPPEIAEQDDRIRRAIGKANCTKFLANFPDGLQTMVGARGAQLSGGQKQRIALARAFLLDPAVLILDEATSALDSQSEEIVAKNLQRRVERGFTTISIAHRLSTIKHSTRVIVLGKHGSVVETGSFRDLIAIPNSELNALLAEQQDEE 679 HSP 2 Score: 226.483 bits (576), Expect = 2.674e-62 Identity = 164/522 (31.42%), Postives = 273/522 (52.30%), Query Frame = 2
Query: 2471 RTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLT-----QAAIFCAYAATFML-AGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPIN-SFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIF-TDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALD-KAQEGRTCVVIAHRLSTIQNADKILVF-KDGKIAESGTHFELLS 4006
+ + AL + ++ V++ + + +GE+L R R+ T +A L QD + D +N +G L +RLS +A+ + +++ AI + W+++ V++ P + ++ R ++ S + ++ K+A E + RT+ E ++ Y + ++ + K+A G +G T A + T M+ +G + + +S + G+ +FG + + KG AAA +FEL DR P I + D V L + I +NV F+YP RP I D++ I + + VGPSG GKSTI LL R+YD +G+I + I++ N++ R+L V QEP LF+ +I +NI Y +I DD+I A +N F+ N PD +T VG +G+ SGGQKQRIA+ARA L +P +L+LDEATSALD++SE+IV L + + G T + IAHRLSTI+++ +++V K G + E+G+ +L++
Sbjct: 153 KQFFTALGAVFIIGAVANASRIIILKVTGERLVARLRTRTMKAALDQDATFLD--TNRVGDLISRLSSDASIVAKSVTQNVSDGTRAIIQGFVGFGMMSFLSWKLTCVMMILAPPLGAMALIYGRKIRNLSRQLQTSVGGLTKVAEEQLNATRTIQAYGGEKNEVRRYAKEVRNVFHIGLKEAVTSGLFFGSTGLVGNTAMLSLLLVGTSMIQSGSMTVGELSSFMMYAVYTGSSLFGLSSFYSELM------KGAGAAARVFELNDRKPLIRPTIGKDPVSLAQK----PIVFKNVSFTYPTRPKHQIFKDLNITIKPGEHVCAVGPSGSGKSTIASLLLRYYDVNSGSIEFGDEDIRNFNLRKYRRLIGYVQQEPLLFNGTILDNILYCIPPEIAEQDDRIRRAIGKANCTKFLANFPDGLQTMVGARGAQLSGGQKQRIALARAFLLDPAVLILDEATSALDSQSEEIVAKNLQRRVERGFTTISIAHRLSTIKHSTRVIVLGKHGSVVETGSFRDLIA 662
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Yeast
Match: MDL2 (Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis [Source:SGD;Acc:S000006191]) HSP 1 Score: 250.751 bits (639), Expect = 6.723e-70 Identity = 164/489 (33.54%), Postives = 250/489 (51.12%), Query Frame = 1
Query: 670 ITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFN----EARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG-RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIV--AHRLSTVRNA-HVIFGIKDGKVQEQGTHAELMA 2112
I S+ ++ +R + + Q+ +FD G L ++L D V + K+ ++ C + G+ + +L+++ L +P ++ + K + ++ EA G+ +AEE L I+T+ SF E E++RY + + ++G + L S+ YG+ L+ + +GD+ + ++ T G A + +F + AA+ +FE+ DR P I G K +G I FK V F+YPTR I L+ I PG +V +VG SG GKS++ L+ R+Y+ G + ID+ +I LN LR IG+V QEPVL +I++NI YG T EEI FI PN DT++G GT LSGGQKQRIAIARAL++ P IL+LDEATSALD ESE + ++++ ++ IV AHRLST+R + +VI DG V E G EL A
Sbjct: 194 ILSERVVARLRANVIKKTLHQDAEFFDNHKVGDLISRLGSDAYVVSRSMTQKVSDGVKALICGVVGVGMMCSLSPQLSILLLFFTPPVLFSASVFGKQIRNTSKDLQEATGQLTRVAEEQLSGIKTVQSFVAEGNELSRYNVAIRDIFQVGKTAAFTNAKFFTTTSLLGDLSFLTVLAYGSYLVLQSQ-LSIGDLT----AFMLYTEYTGNAVFGLSTFYSEIMQGAGAASRLFELTDRKPSISPT--VGHKYKPDRGVIEFKDVSFSYPTRPSVQIFKNLNFKIAPGSSVCIVGPSGRGKSTIALLLLRYYNPTTGTITIDNQDISKLNCKSLRRHIGIVQQEPVLMSGTIRDNITYGLTYTPTKEEIRSVAKQCFCHNFITKFPNTYDTVIGPHGTLLSGGQKQRIAIARALIKKPTILILDEATSALDVESEGAINYTFGQLMKSKSMTIVSIAHRLSTIRRSENVIVLGHDGSVVEMGKFKELYA 675 HSP 2 Score: 217.238 bits (552), Expect = 9.077e-59 Identity = 169/497 (34.00%), Postives = 259/497 (52.11%), Query Frame = 2
Query: 2558 EQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQI-RGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVP-IIFVGGIL--QIRIMQGFSNSDKGALEESG---KIAVECIENIRTVSNLTREHTFIQHYKEAIKKL--DGEVSKKAHAQGF----LYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGR--TCVVIAHRLSTIQNADKILVF-KDGKIAESGTHFEL 4000
E++ R R+ + L QD +FD ++ +G L +RL +A + R ++ + V IC + V + Q+S+++L F P ++F + QIR N+ K E +G ++A E + I+TV + E + Y AI+ + G+ + +A+ F L G AY + +L L + +++ G +FG + +G AA+ +FEL DR P I+ D G I+ ++V FSYP RP++ I +++F I ++ +VGPSG GKSTI LL R+Y+ G I IDN I +N ++LR+ IV QEP L S +IR+NI YG + T ++I + AK H+FI P+ Y+T +G G+ SGGQKQRIAIARAL++ P IL+LDEATSALD ESE + + + + T V IAHRLSTI+ ++ ++V DG + E G EL
Sbjct: 197 ERVVARLRANVIKKTLHQDAEFFD--NHKVGDLISRLGSDAYVVSRSMTQKVSDGVKALICGV-VGVGMMCSLSPQLSILLLFFTPPVLFSASVFGKQIR------NTSKDLQEATGQLTRVAEEQLSGIKTVQSFVAEGNELSRYNVAIRDIFQVGKTAAFTNAKFFTTTSLLGDLSFLTVLAYGSYLVLQSQLSIGDLTAFMLYTEYTGNAVFGLSTFYSEIM------QGAGAASRLFELTDRKPSISPTVGHKYKPD----RGVIEFKDVSFSYPTRPSVQIFKNLNFKIAPGSSVCIVGPSGRGKSTIALLLLRYYNPTTGTITIDNQDISKLNCKSLRRHIGIVQQEPVLMSGTIRDNITYGLTYTP-TKEEIRSVAKQCFCHNFITKFPNTYDTVIGPHGTLLSGGQKQRIAIARALIKKPTILILDEATSALDVESEGAINYTFGQLMKSKSMTIVSIAHRLSTIRRSENVIVLGHDGSVVEMGKFKEL 673
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Yeast
Match: STE6 (Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells; human homolog ABCB1 mediates multidrug resistance in many chemotherapy-resistant tumors by effluxing toxic compounds from the cell [Source:SGD;Acc:S000001692]) HSP 1 Score: 211.846 bits (538), Expect = 2.204e-55 Identity = 159/591 (26.90%), Postives = 286/591 (48.39%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSAD---IRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVIT--AICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL---YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGD-----------------IKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
+ LG + ++ GA P FS F FLL + P+ + ++ + + + + G+A + + + E R+ E + ++++ WF +N ++ + ++ +R + E L A+ + + ++G A+ G W++SLV ++ P+I + + I+Q K ++ + + + NI+T+ L E F Y + K+ SK+A A GF +T + C A + L++ Y +M F I +L ++ P+ +G+ AA+ ++ +LD L+VEN + + I+N+ F+YP P + +++F++ QT+ ++G SG GKST++ LL + Y+ G I ID + D N+ +LR+ S+V Q+P LF+ +IR+N+ YG +I + ++ A K IHDF+++ P +T++ + SGGQ QR+ IARALLR +IL+LDE TSALD+ S I+ + + K +VI H +++ + I V KDGK+ E G L + G Q++S QS
Sbjct: 717 LILGLLCSLIAGATNPVFSYTFSFLLEGIV-PSTDGKTGSSHYLAKWSLLVLGVAAADGIFNFAKGFLLDCCSEYWVMDLRNEVMEKLTRKNMDWFSGENNKASEISALVLNDLRDLRSLVSEFLSAMTSFVTVSTIGLIWALVSG--WKLSLVCISMFPLIIIFSAIYGGILQKCETDYKTSVAQLENCLYQIVTNIKTIKCLQAEFHFQLTYHDLKIKMQQIASKRAIATGFGISMTNMIVMCIQAIIYYYGLKLVMIHEYTSKEMFTTFTLLLFTIMSCTSL---VSQIPDISRGQRAASWIYRILDEKHN---------TLEVENNNARTVGIAGHTYHGKEKKPIVSIQNLTFAYPSAPTAFVYKNMNFDMFCGQTLGIIGESGTGKSTLVLLLTKLYNCEVGKIKIDGTDVNDWNLTSLRKEISVVEQKPLLFNGTIRDNLTYGLQDEIL-EIEMYDALKYVGIHDFVISSPQGLDTRIDT--TLLSGGQAQRLCIARALLRKSKILILDECTSALDSVSSSIINEIVKKGPPALLTMVITHSEQMMRSCNSIAVLKDGKVVERGNFDTLYNNRGELFQIVSNQS 1289 HSP 2 Score: 201.06 bits (510), Expect = 5.104e-52 Identity = 147/508 (28.94%), Postives = 251/508 (49.41%), Query Frame = 1
Query: 646 YVSVTFFVITSQHQISAIRNAFFRAVMRQN-IGWFDAQNS--GALT--NKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIID----RVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDV-------TDEE---IDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMA 2112
++S+T ++ + Q IR+ A + + + W+D G T N+ V+++ + A Q + +G +F W LTL+ L SP+I ++ S+ + ++K + KA + + + + + + ++ E ++ E + I+ G++ L + GFW+G+++I++G + D++ F SC+M + + + + A +I ++ R P ++K L+ +TF V F+YP+R +L +SL+ GQ +VG SG GKS+L L+ RFYD G + I+ NI+ ++ L E I VV Q LF ++++NI G D T+E I A MA FI LP+ L+TL+G G LSGGQ+QR+AIARA +R+ IL LDEA SALD ++ A+ +G+TTII+ H LS + + ++ +K+G+V E GT +EL+A
Sbjct: 90 WLSLTSWMHIGERQGFRIRSQILEAYLEEKPMEWYDNNEKLLGDFTQINRCVEELRSSSAEASAITFQNLVAICALLGT---SFYYSWSLTLIILCSSPIITFFAVVFSRMIHVYSEKENSETSKAAQLLTWSMNAAQLVRLYCTQRLERKKFKEIILNCNTFFIKSCFFVAANAGILRFLTLTMFVQGFWFGSAMIKKGK-LNINDVITCFHSCIMLGSTLNNTLHQIVVLQKGGVAMEKIMTLLKDGSKRNP-LNKTVAHQFPLDYATSDLTFANVSFSYPSRPSEAVLKNVSLNFSAGQFTFIVGKSGSGKSTLSNLLLRFYDGYNGSISINGHNIQTIDQKLLIENITVVEQRCTLFNDTLRKNILLGSTDSVRNADCSTNENRHLIKDACQMALLDRFILDLPDGLETLIGTGGVTLSGGQQQRVAIARAFIRDTPILFLDEAVSALDIVHRNLLMKAIRHWRKGKTTIILTHELSQIESDDYLYLMKEGEVVESGTQSELLA 592 HSP 3 Score: 200.29 bits (508), Expect = 7.624e-52 Identity = 147/528 (27.84%), Postives = 266/528 (50.38%), Query Frame = 1
Query: 616 GIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGA--LTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASL--IREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDR---VPEIDKMNEE--GDKLNDFQGK-----ITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD-VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKN 2154
G+A A I ++ S++ + +RN + R+N+ WF +N+ A ++ +++D+ + + + + + M + G+I A V GWKL+LV +S+ PLIII + + K + + +++ +I+TI + E + Y + + +I +++I TG + + ++ A+ ++YG L I E + + +M+ T + P + + + AA+ I+ I+D E++ N G + + GK ++ + + F YP+ + ++ + GQT+ ++G SG GKS+L+ L+ + Y+ G++ ID T++ D N+ LR++I VV Q+P+LF +I++N+ YG D + + E+ AL F+ + P LDT + T LSGGQ QR+ IARAL+R KIL+LDE TSALDS S I+ + + K T+++ H +R+ + I +KDGKV E+G L G +V Q +
Sbjct: 768 GVAAADGIFNFAKGFLLDCCSEYWVMDLRNEVMEKLTRKNMDWFSGENNKASEISALVLNDLRDLRSLVSEFLSAMTSFVTVSTIGLIWALVSGWKLSLVCISMFPLIIIFSAIYGGILQKCETDYKTSVAQLENCLYQIVTNIKTIKCLQAEFHFQLTYHDLKIKMQQIASKRAIATGFGISMTNMIVMCIQAIIYYYGLKLVMIHEYTSKEMFTTFTLLLFTIMSCTSLVSQIPDI---SRGQRAASWIYRILDEKHNTLEVENNNARTVGIAGHTYHGKEKKPIVSIQNLTFAYPSAPTAFVYKNMNFDMFCGQTLGIIGESGTGKSTLVLLLTKLYNCEVGKIKIDGTDVNDWNLTSLRKEISVVEQKPLLFNGTIRDNLTYGLQDEILEIEMYDALKYVGIHDFVISSPQGLDTRIDT--TLLSGGQAQRLCIARALLRKSKILILDECTSALDSVSSSIINEIVKKGPPALLTMVITHSEQMMRSCNSIAVLKDGKVVERGNFDTLYNNRGELFQIVSNQSS 1290 HSP 4 Score: 192.971 bits (489), Expect = 1.221e-49 Identity = 141/496 (28.43%), Postives = 243/496 (48.99%), Query Frame = 2
Query: 2555 GEQLTKRCRSITFEAMLKQD-IAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELL---DRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDD--------QIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLS 4006
GE+ R RS EA L++ + W+D +G T +++ ++R S E+ + ++ A + SF + W ++L++L PII ++ R++ +S + ++ ++ + + V + + +KE I + K G+ + + F ++ + +V F + I L ++ KG +A + LL + P+N LD + D+ NV FSYP RP+ +L +VS N ++ Q +VG SG GKST+ LL RFYD NG+I I+ + I+ I+ + L + ++V Q TLF+ ++R+NI G + + D I A + + + FI++LPD ET +G G SGGQ+QR+AIARA +R+ IL LDEA SALD ++ A+ ++G+T +++ H LS I++ D + + K+G++ ESGT ELL+
Sbjct: 100 GERQGFRIRSQILEAYLEEKPMEWYDNNEKLLGDFT-QINRCVEELRSSSAEASAITFQNLVAICALLGTSFYYSWSLTLIILCSSPIITFFAVVFSRMIHVYSEKENSETSKAAQLLTWSMNAAQLVRLYCTQRLERKKFKEIILNCNTFFIKSCFFVAANAGILRFLTLTMFVQGFWFGSAMIKKGKLNINDVITCFHSCIMLGSTLNNTLHQIVVLQKGGVAMEKIMTLLKDGSKRNPLNKTVAHQFPLDYA--TSDLTFANVSFSYPSRPSEAVLKNVSLNFSAGQFTFIVGKSGSGKSTLSNLLLRFYDGYNGSISINGHNIQTIDQKLLIENITVVEQRCTLFNDTLRKNILLGSTDSVRNADCSTNENRHLIKDACQMALLDRFILDLPDGLETLIGTGGVTLSGGQQQRVAIARAFIRDTPILFLDEAVSALDIVHRNLLMKAIRHWRKGKTTIILTHELSQIESDDYLYLMKEGEVVESGTQSELLA 592
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Yeast
Match: ATM1 (Mitochondrial inner membrane ATP-binding cassette (ABC) transporter; exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol; human homolog ABCB7 can complement yeast null mutant [Source:SGD;Acc:S000004916]) HSP 1 Score: 199.904 bits (507), Expect = 1.506e-53 Identity = 137/427 (32.08%), Postives = 223/427 (52.22%), Query Frame = 1
Query: 871 ILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKL--NDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEK-IVQDALDKVVEG-RTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNG-LYKDL 2136
IL + G +T S L I + + + A++A KA +A + L + + F EKY +Y +L I+ S L + + + G + + GN VGD++++ + + ++ +F++ R E+ N E + + ITF+ V F Y D IL S +I G A+VG+SG GKS++++L+ RFYD G +LI+ +IK+ ++ LR+ IGVV Q+ LF +I EN+++GR+D TDEE+ + A I LP DT+VG+RG +SGG+KQR+AIAR L++N +I+ DEATSALD+ +E+ +++ D G RT++ +AHRL T+ +A I + +G+V+E+G H EL+A+ G LY++L
Sbjct: 249 ILTYQFGASFAAITFSTMLLYSIFTIKTTAWRTHFRRDANKADNKAASVALDSLINFEAVKYFNNEKYLADKYNGSLMNYRDSQIKVSQSLAFLNSGQNLIFTTALTAMMYMGCTGVIGGN-LTVGDLVLINQLVFQLSVPLNFLGSVYRDLKQSLIDMETLFKL--RKNEVKIKNAERPLMLPENVPYDITFENVTFGY--HPDRKILKNASFTIPAGWKTAIVGSSGSGKSTILKLVFRFYDPESGRILINGRDIKEYDIDALRKVIGVVPQDTPLFNDTIWENVKFGRIDATDEEVITVVEKAQLAPLIKKLPQGFDTIVGERGLMISGGEKQRLAIARVLLKNARIMFFDEATSALDTHTEQALLRTIRDNFTSGSRTSVYIAHRLRTIADADKIIVLDNGRVREEGKHLELLAMPGSLYREL 670 HSP 2 Score: 184.111 bits (466), Expect = 2.346e-48 Identity = 157/515 (30.49%), Postives = 254/515 (49.32%), Query Frame = 2
Query: 2528 LQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFG---FCWQISLVVLAFVPIIFVGGILQIRIM---QGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFG-AMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEK-IVQDALDKAQEG-RTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNG-IYSQMISLQ 4042
L+N F + + TF+ ++K D+ W S G LT R D T +G+S + L A++ I + +++ G + + S + F ++ I I+ F A ++ +A++ + N V E Y ++ K + + FL Q IF M G + + + L+F ++ L ++ + + I +F+L R + + + ++ EN+ DI ENV F Y P+ IL + SF I + A+VG SG GKSTI+ L+ RFYD +G ILI+ IK+ +I LR++ +V Q+ LF+ +I EN+ +G TD+++I + + + I LP ++T VGE+G SGG+KQR+AIAR LL+N RI+ DEATSALDT +E+ +++ D G RT V IAHRL TI +ADKI+V +G++ E G H ELL+ G +Y ++ ++Q
Sbjct: 174 LRNAVFAKVAQNAIRTVSLQTFQHLMKLDLGW--HLSRQTGGLT-RAMDRGT--KGIS-QVLTAMVFHIIPISFEISVVCGILTYQFGASFAAITFSTMLLYS-IFTIKTTAWRTHFRRDANKADNKAASVALDSLINFEAVKYFNNEKYLADKYNGSLMNYRDSQIKVSQSLAFLNS-GQNLIFTTALTAMMYMGCTGVIGGNLTVGDLVLINQLVFQLSVPLNFLGSVYRDLKQSLIDMETLFKL--RKNEVKIKNAERPLMLPENVPYDITFENVTFGY--HPDRKILKNASFTIPAGWKTAIVGSSGSGKSTILKLVFRFYDPESGRILINGRDIKEYDIDALRKVIGVVPQDTPLFNDTIWENVKFGRIDA--TDEEVITVVEKAQLAPLIKKLPQGFDTIVGERGLMISGGEKQRLAIARVLLKNARIMFFDEATSALDTHTEQALLRTIRDNFTSGSRTSVYIAHRLRTIADADKIIVLDNGRVREEGKHLELLAMPGSLYRELWTIQ 674
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Yeast
Match: YCF1 (Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR [Source:SGD;Acc:S000002542]) HSP 1 Score: 141.354 bits (355), Expect = 1.026e-33 Identity = 89/234 (38.03%), Postives = 148/234 (63.25%), Query Frame = 2
Query: 3317 GDIKIENVKFSYPLRPNI-IILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLP-DQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKN 4012
GDIK N Y RP + ++L ++ +I N+ + +VG +G GKS++ L R + GNI+IDN I +I + +LR SI+ Q+ +F ++RENI D +TD+ I A + S++ + ++++ D + ++ E G N S GQ+Q + +ARA+L +IL+LDEAT+A+D E++K+VQ+ + A + RT + IAHRL+TI ++D+I+V +GK+AE + +LLS N
Sbjct: 1270 GDIKFNNYSTRY--RPELDLVLKHINIHIKPNEKVGIVGRTGAGKSSLTLALFRMIEASEGNIVIDNIAINEIGLYDLRHKLSIIPQDSQVFEGTVRENI---DPINQYTDEAIWRALELSHLKEHVLSMSNDGLDAQLTEGGGNLSVGQRQLLCLARAMLVPSKILVLDEATAAVDVETDKVVQETIRTAFKDRTILTIAHRLNTIMDSDRIIVLDNGKVAEFDSPGQLLSDN 1498 HSP 2 Score: 134.035 bits (336), Expect = 1.971e-31 Identity = 78/232 (33.62%), Postives = 140/232 (60.34%), Query Frame = 1
Query: 1426 QGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNK-LDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALN 2118
QG I F Y D +L +++ I+P + V +VG +G GKSSL + R +++EG ++ID+ I ++ + LR ++ ++ Q+ +F +++ENI TDE I +AL +++ K + ++ N LD + + G LS GQ+Q + +ARA++ KIL+LDEAT+A+D E++K+VQ+ + + RT + +AHRL+T+ ++ I + +GKV E + +L++ N
Sbjct: 1269 QGDIKFNNYSTRYRPELDL-VLKHINIHIKPNEKVGIVGRTGAGKSSLTLALFRMIEASEGNIVIDNIAINEIGLYDLRHKLSIIPQDSQVFEGTVRENID-PINQYTDEAIWRALELSHLKEHVLSMSNDGLDAQLTEGGGNLSVGQRQLLCLARAMLVPSKILVLDEATAAVDVETDKVVQETIRTAFKDRTILTIAHRLNTIMDSDRIIVLDNGKVAEFDSPGQLLSDN 1498 HSP 3 Score: 74.3294 bits (181), Expect = 2.690e-13 Identity = 69/276 (25.00%), Postives = 128/276 (46.38%), Query Frame = 1
Query: 1315 MGSFNEARSAAAEIFEII-------DRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPI-LTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTF-INALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSE-SEKIVQDAL--DKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAEL 2106
+ SF EA + +F D V + K+ GD + TF + + + + + L ++ + G +VG G GK++L+ M L D +K + + VSQ P + ++KENI +G D E + + A A T + L + TLVG++G LSGGQK R+++ARA+ LLD+ +A+D + +++ L + ++ +T ++ +++S + A I + +G++ +QGT+ E+
Sbjct: 585 LNSFIEASVSIGRLFTFFTNEELQPDSVQRLPKVKNIGDVAINIGDDATFLWQR-----KPEYKVALKNINFQAKKGNLTCIVGKVGSGKTALLSCM-----------LGDLFRVKGFATV--HGSVAYVSQVPWIMNGTVKENILFGHR--YDAEFYEKTIKACALTIDLAILMDGDKTLVGEKGISLSGGQKARLSLARAVYARADTYLLDDPLAAVDEHVARHLIEHVLGPNGLLHTKTKVLATNKVSALSIADSIALLDNGEITQQGTYDEI 840 HSP 4 Score: 65.855 bits (159), Expect = 8.373e-11 Identity = 58/236 (24.58%), Postives = 111/236 (47.03%), Query Frame = 2
Query: 3317 GDIKI---ENVKFSYPLRPNI-IILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKI-FTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTE-SEKIVQDAL--DKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFEL 4000
GD+ I ++ F + +P + L +++F +VG G GK+ ++ + + + + + VSQ P + + +++ENI +G F + I A A ++ L D +T VGEKG + SGGQK R+++ARA+ LLD+ +A+D + +++ L + +T V+ +++S + AD I + +G+I + GT+ E+
Sbjct: 622 GDVAINIGDDATFLWQRKPEYKVALKNINFQAKKGNLTCIVGKVGSGKTALLSCM-------------LGDLFRVKGFATVHGSVAYVSQVPWIMNGTVKENILFGHRYDAEFYEKTIKACALTIDL----AILMDGDKTLVGEKGISLSGGQKARLSLARAVYARADTYLLDDPLAAVDEHVARHLIEHVLGPNGLLHTKTKVLATNKVSALSIADSIALLDNGEITQQGTYDEI 840
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Nematostella
Match: EDO49737 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFK3]) HSP 1 Score: 532.332 bits (1370), Expect = 1.070e-161 Identity = 272/520 (52.31%), Postives = 363/520 (69.81%), Query Frame = 1
Query: 589 MSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
M+ ++ + +A+ I++Y+ FF T+ Q IR FF+AVMRQ+IGWFD ++G L N+L +DI+ V G+G K+G ++Q+ F+ G I+ F WKLTLV L+++PL++I ++ K + T K EAY KAG IAEEVL SIRT+ +F GEK E RY +L EA G++K + TG G +MFGSY+L FWYGA L+ + GD+L+VFFS ++ T +GQA P++ + AR AA E++ IIDR P ID +EEG K +G I F + F YP+R D +L GL L+I+ GQTVALVG SGCGKS+LI+L+QRFYD EG V +D +I+ LN+ WLR+ IGVVSQEP+LF ++ ENIRYGR +T EI++A MANA FI LP +T+VG+RG Q+SGGQKQRIAIARALV+NP +L+LDEATSALD+ESEKIVQ ALDK EGRTT+++AHRLST+RNA VI I+DG V E+G+H ELMA +GLY+ L+ Q
Sbjct: 1 MTDLAIYYIYLAIGALIVAYLQAGFFQYTAVRQAKRIRCNFFKAVMRQDIGWFDTYDAGELNNRLTEDISKVVDGLGSKVGLVVQFTTTFLAGFIMGFAYSWKLTLVILALTPLMVIAGGIMGKVISVFTSKELEAYAKAGAIAEEVLSSIRTVAAFGGEKKECERYNSHLGEAQAFGVKKGLSTGLGFGFFQLIMFGSYSLAFWYGAVLVAD-KAINSGDLLVVFFSVMVGATQLGQAGPNIEAIATARGAAYELYSIIDRQPPIDSSSEEGLKPASVKGDIDFTDIHFQYPSRPDVKVLKGLHLTIRSGQTVALVGESGCGKSTLIKLVQRFYDPAEGTVCMDGIDIRSLNLKWLRQHIGVVSQEPILFATTVAENIRYGREGITQAEIEKATKMANAHDFIRNLPQGYNTVVGERGAQMSGGQKQRIAIARALVKNPTLLILDEATSALDTESEKIVQAALDKASEGRTTLVIAHRLSTIRNATVIAAIQDGVVVEKGSHNELMATDGLYRQLITLQ 519 HSP 2 Score: 489.189 bits (1258), Expect = 7.114e-147 Identity = 252/578 (43.60%), Postives = 375/578 (64.88%), Query Frame = 2
Query: 2309 FKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
+V+ +N+ + + +G +SA+ NG + SF++L G +L N ++ + + + + +F + + QN F SGE LT + R ++F+++L+Q++A+FD+P + GALTT L+ A+ ++G +G LG + + ++ A+ +F W++SLVV AF+P I + G L ++ G + K ESGKIAVE EN+RT++ L REHTF +HY +I ++AH G YGLT+A +F AA F YL++ M V ++ + G+ +++P++ K + AA +F+LLDR P I+S S +G L + G +++ +V+F YP RPN+ +L +S +N QT+ALVGPSGCGKST + LL+RFYD +G + IDN ++ +N++ LR IVSQEP LF YSI +NIAYGD+++ + +I AAKA+NIH+FI LP Y+T+VG+KG+ SGGQKQRIAIARAL+RNP ILLLDEATSALDTESEK+VQDALD A EGRT ++IAHRLST++NAD I V G++AE GTH EL++ NGIY+ +++ Q
Sbjct: 537 LRVLRMNSDQWPVMVVGVISALINGLLPMSFALLLGEILNVFTLVNTDEMKKEATFWALMFLVMGGASFFTQIFQNYMFAISGEALTVKIRRLSFKSLLRQEMAFFDDPFHTTGALTTALATHASDVKGAAGSRLGTLALGLSTVVASAIYAFYNGWKLSLVVCAFIPFIVLAGALHMKAFTG-DHGGKDDYIESGKIAVEAFENVRTIATLGREHTFFEHYSRSIDGPHKVAVRRAHLSGASYGLTEAIMFLCNAACFRFGAYLIVQGEMDMPRVMKVVMCIVIAGLVAGQISSLSPDYQKARTAAGKIFKLLDRTPAIDSASENG--LQPAAVRGTVQVRSVRFKYPTRPNVKVLRGLSLEVNQGQTLALVGPSGCGKSTTVSLLERFYDPEDGEMAIDNANVRQLNLKWLRSKIGIVSQEPVLFGYSIAQNIAYGDNSREVSMAEIETAAKAANIHNFICGLPKGYDTEVGDKGTLISGGQKQRIAIARALIRNPPILLLDEATSALDTESEKVVQDALDAASEGRTVIMIAHRLSTVKNADVICVIDHGRVAEQGTHQELMAMNGIYTGLVTAQ 1111 HSP 3 Score: 393.275 bits (1009), Expect = 2.532e-114 Identity = 230/538 (42.75%), Postives = 324/538 (60.22%), Query Frame = 1
Query: 574 NIFTLMSG--MSQQFAGIALAMFIM---SYVSVTF----FVITSQHQISAIRNAFFRAVMRQNIGWFDA--QNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
N+FTL++ M ++ AL +M S+ + F F I+ + IR F++++RQ + +FD +GALT L + V G ++G + + + I AF GWKL+LV + P I++ + K + Y ++G IA E ++RTI + E Y+ ++D K+ +R++ L+G GL +MF A F +GA LI +G + M +V ++A GQ + + +AR+AA +IF+++DR P ID +E G + +G + + V+F YPTR + +L GLSL + GQT+ALVG SGCGKS+ + L++RFYD +GE+ ID+ N++ LN+ WLR +IG+VSQEPVLFG SI +NI YG +V+ EI+ A AN FI LP DT VGD+GT +SGGQKQRIAIARAL+RNP ILLLDEATSALD+ESEK+VQDALD EGRT I++AHRLSTV+NA VI I G+V EQGTH ELMA+NG+Y LV Q
Sbjct: 576 NVFTLVNTDEMKKEATFWALMFLVMGGASFFTQIFQNYMFAISGEALTVKIRRLSFKSLLRQEMAFFDDPFHTTGALTTALATHASDVKGAAGSRLGTLALGLSTVVASAIYAFYNGWKLSLVVCAFIPFIVLAGALHMKAFTGD-HGGKDDYIESGKIAVEAFENVRTIATLGREHTFFEHYSRSIDGPHKVAVRRAHLSGASYGLTEAIMFLCNAACFRFGAYLIVQGEMDMPRVMKVVM-CIVIAGLVAGQISSLSPDYQKARTAAGKIFKLLDRTPAIDSASENGLQPAAVRGTVQVRSVRFKYPTRPNVKVLRGLSLEVNQGQTLALVGPSGCGKSTTVSLLERFYDPEDGEMAIDNANVRQLNLKWLRSKIGIVSQEPVLFGYSIAQNIAYGDNSREVSMAEIETAAKAANIHNFICGLPKGYDTEVGDKGTLISGGQKQRIAIARALIRNPPILLLDEATSALDTESEKVVQDALDAASEGRTVIMIAHRLSTVKNADVICVIDHGRVAEQGTHQELMAMNGIYTGLVTAQ 1111 HSP 4 Score: 372.859 bits (956), Expect = 1.667e-107 Identity = 211/532 (39.66%), Postives = 318/532 (59.77%), Query Frame = 2
Query: 2465 DIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ---SHK 4051
D+ Y I LA + A + + LQ F + + KR R F+A+++QDI WFD + + G L RL+++ +++ G +G V+ + A + F + W+++LV+LA P++ + G + +++ F++ + A ++G IA E + +IRTV+ E + Y + + KK + G +G Q +F +Y+ F L+ ++ + F +++ GA LG++ + AA ++ ++DR PPI+S S +G L ++ GDI ++ F YP RP++ +L + I S QT+ALVG SGCGKST+I L+QRFYD G + +D I+ +N++ LRQ +VSQEP LF+ ++ ENI YG T +I A K +N HDFI NLP Y T VGE+G+ SGGQKQRIAIARAL++NP +L+LDEATSALDTESEKIVQ ALDKA EGRT +VIAHRLSTI+NA I +DG + E G+H EL++ +G+Y Q+I+LQ +HK
Sbjct: 3 DLAIYYIYLA---IGALIVAYLQAGFFQYTAVRQAKRIRCNFFKAVMRQDIGWFD--TYDAGELNNRLTEDISKVVDGLGSKVGLVVQFTTTFLAGFIMGFAYSWKLTLVILALTPLMVIAGGIMGKVISVFTSKELEAYAKAGAIAEEVLSSIRTVAAFGGEKKECERYNSHLGEAQAFGVKKGLSTGLGFGFFQLIMFGSYSLAFWYGAVLVADKAINSGDLLVVFFSVMVGATQLGQAGPNIEAIATARGAAYELYSIIDRQPPIDSSSEEG--LKPASVKGDIDFTDIHFQYPSRPDVKVLKGLHLTIRSGQTVALVGESGCGKSTLIKLVQRFYDPAEGTVCMDGIDIRSLNLKWLRQHIGVVSQEPILFATTVAENIRYGREG--ITQAEIEKATKMANAHDFIRNLPQGYNTVVGERGAQMSGGQKQRIAIARALVKNPTLLILDEATSALDTESEKIVQAALDKASEGRTTLVIAHRLSTIRNATVIAAIQDGVVVEKGSHNELMATDGLYRQLITLQGKHNHK 525
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Nematostella
Match: EDO48846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHG7]) HSP 1 Score: 476.863 bits (1226), Expect = 1.135e-145 Identity = 252/587 (42.93%), Postives = 382/587 (65.08%), Query Frame = 2
Query: 2300 AGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTY----VIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALE--ESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
A F +++ LN+PE +I +G +SA+ NG + F++ G +L + + ++ + T+ +A+ GI+ F + + QN F SGE LT R R ++ +A+L+Q+IA+FD+P ++ GALTT L+ + ++G +G LG + T I ++ A+ + W+++L VLAFVP + +G L+I+ G++ G + ESGK+AVE EN+RT++ L RE TF + Y + + + +AH G YG+T+A ++ +AA F +L++ M V ++ M G+ A +P++ K K+A A +F+L+DR+P I+S S +G L + G +++ +++F YP RP++ +L +S + QT+ALVGPSGCGKST + LL+RFYD G + +DN ++ +NI+ LR IVSQEP LF YSI +NIAYGD+++ + +I AA+A+NIHDFI LP YET+VG+KG+ SGGQKQRIAIARAL++NP ILLLDEATSALDTESEK+VQDALD A EGRT ++IAHRLST++NAD I V G++AE GTH EL++ NGIY+ +++ Q
Sbjct: 264 APFLRILKLNSPEWVFIAIGALSAMANGFLPLGFALCLGEILTVFTITDQKDKAKEEATFWALMFLAMGGIS---FFTQLFQNYMFALSGEALTVRLRRMSLKALLRQEIAFFDDPMHSTGALTTALATHTSDVKGAAGSRLGTIATGISTVIASAIYASINGWKLTLAVLAFVPFLAIGRALEIK---GYTGDTGGQNDYIESGKVAVEAFENVRTIAILGREKTFFERYAQTLVRPHRAAVVRAHLFGAGYGVTEAIMYFCFAACFRYGAHLMVEKEMTMEEVMKVVMCIMIAGMVAGELFAFSPDYLKAKVAGARIFKLVDRIPVIDSASDEG--LTPSTVQGTLQMRSLRFRYPARPDVKVLRGLSLEVKQGQTLALVGPSGCGKSTTVSLLERFYDPEEGKVAVDNRDVRQLNIKWLRSKIGIVSQEPVLFGYSIAQNIAYGDNSREVSMKEIETAARAANIHDFISGLPKGYETEVGDKGTLLSGGQKQRIAIARALVKNPPILLLDEATSALDTESEKVVQDALDAASEGRTVIMIAHRLSTVKNADVICVIDHGRVAEHGTHQELMAMNGIYTGLVTAQ 842 HSP 2 Score: 378.637 bits (971), Expect = 6.887e-112 Identity = 218/516 (42.25%), Postives = 312/516 (60.47%), Query Frame = 1
Query: 625 LAMFIMSYVSVTF----FVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
LAM +S+ + F F ++ + +R +A++RQ I +FD ++GALT L + V G ++G + + I I A + GWKLTL L+ P + I + K T ++ Y ++G +A E ++RTI EK RY + L + + ++ L G G+ +M+ +A F YGA L+ E + +++ V ++A G+ + +A+ A A IF+++DR+P ID ++EG + QG + + ++F YP R D +L GLSL ++ GQT+ALVG SGCGKS+ + L++RFYD EG+V +D+ +++ LN+ WLR +IG+VSQEPVLFG SI +NI YG +V+ +EI+ A AN FI+ LP +T VGD+GT LSGGQKQRIAIARALV+NP ILLLDEATSALD+ESEK+VQDALD EGRT I++AHRLSTV+NA VI I G+V E GTH ELMA+NG+Y LV Q
Sbjct: 329 LAMGGISFFTQLFQNYMFALSGEALTVRLRRMSLKALLRQEIAFFDDPMHSTGALTTALATHTSDVKGAAGSRLGTIATGISTVIASAIYASINGWKLTLAVLAFVPFLAIGRALEIKGYTGDTGGQND-YIESGKVAVEAFENVRTIAILGREKTFFERYAQTLVRPHRAAVVRAHLFGAGYGVTEAIMYFCFAACFRYGAHLMVEKE-MTMEEVMKVVMCIMIAGMVAGELFAFSPDYLKAKVAGARIFKLVDRIPVIDSASDEGLTPSTVQGTLQMRSLRFRYPARPDVKVLRGLSLEVKQGQTLALVGPSGCGKSTTVSLLERFYDPEEGKVAVDNRDVRQLNIKWLRSKIGIVSQEPVLFGYSIAQNIAYGDNSREVSMKEIETAARAANIHDFISGLPKGYETEVGDKGTLLSGGQKQRIAIARALVKNPPILLLDEATSALDTESEKVVQDALDAASEGRTVIMIAHRLSTVKNADVICVIDHGRVAEHGTHQELMAMNGIYTGLVTAQ 842 HSP 3 Score: 259.996 bits (663), Expect = 5.984e-72 Identity = 124/196 (63.27%), Postives = 157/196 (80.10%), Query Frame = 1
Query: 1561 SSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
S++I+L+QRFYD G V +D T+I+ LN+ WLR++IGVVSQEP LF +I ENIRYG+ VT EI+QA MANA FI LP +T+VG++G+QLSGGQKQR+AIARALVRNP+IL+LDEATSALD+ESE++VQ ALDK EGRTT+++AHRLSTVRNA +I I +G V E G+HAELMA +G+Y+ LV Q
Sbjct: 1 STVIKLIQRFYDPENGSVCLDGTDIRSLNLHWLRQRIGVVSQEPALFATTIAENIRYGQDGVTQAEIEQAAKMANAHDFITKLPKGYETVVGEQGSQLSGGQKQRVAIARALVRNPRILILDEATSALDTESERVVQAALDKAREGRTTLVIAHRLSTVRNADMIAAIHNGVVVETGSHAELMATDGVYRQLVTLQ 196 HSP 4 Score: 225.328 bits (573), Expect = 1.552e-60 Identity = 116/199 (58.29%), Postives = 151/199 (75.88%), Query Frame = 2
Query: 3449 STIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQS 4045
ST+I L+QRFYD NG++ +D I+ +N+ LRQ +VSQEP LF+ +I ENI YG T +I AAK +N HDFI LP YET VGE+GS SGGQKQR+AIARAL+RNPRIL+LDEATSALDTESE++VQ ALDKA+EGRT +VIAHRLST++NAD I +G + E+G+H EL++ +G+Y Q+++LQ+
Sbjct: 1 STVIKLIQRFYDPENGSVCLDGTDIRSLNLHWLRQRIGVVSQEPALFATTIAENIRYGQDG--VTQAEIEQAAKMANAHDFITKLPKGYETVVGEQGSQLSGGQKQRVAIARALVRNPRILILDEATSALDTESERVVQAALDKAREGRTTLVIAHRLSTVRNADMIAAIHNGVVVETGSHAELMATDGVYRQLVTLQA 197
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Nematostella
Match: EDO41930 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S348]) HSP 1 Score: 355.525 bits (911), Expect = 3.288e-104 Identity = 203/530 (38.30%), Postives = 304/530 (57.36%), Query Frame = 1
Query: 637 IMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILT------------------GLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNRPWTKR 2172
I + + F + Q ++ IR F A+++Q + +FD +G L N+L D + + + ++++ IG ++ F KLT V +S+ P++ + + + L +K + G A +AEE +GSIRT+ SF EK + Y E + E+ +G R +I +G G+VM + + L WYG L+ EG VG + + + G F +A A+ +FE++DRVP+I KM E G L F+ I F V+F+YP+R D +L LS I PG+TVALVG SG GKS++I L++RFYD G + I DT+I L++ W R++I +VSQEPVLF +I NI YGR D + EEI++A ANA FI + T VG+RG +LSGGQKQR+AIARAL+ +P+ILLLDEATSALD+ESE V++A+D+ + RT +++AHRLSTVR+A+++ + GK+ E+G H L+A G+Y+ LV RQ + +R
Sbjct: 277 IAAMIRAWLFTLAGQRLVARIRRELFNAIIKQEVAFFDTNRTGELINRLSSDTQVIQNAVTVNVSMLLRYIVQIIGSLVFMFTLSAKLTGVLISVIPIVGVGAQKYGQFVQGLRKKFQDELGNASTVAEESIGSIRTVRSFSQEKKTMKLYAEAIHESYLVGARLAIASGAFSGVVMAVGQAAIVLVLWYGGKLVNEGE-MNVGILTAFALYTVNVAMAFAFLSSLYGDFMQAVGASFRMFELMDRVPKI-KM-EGGVILPSFKEPIVFDDVRFSYPSREDTEVLKIEEAARQANAHDFITTFEALSFKINPGETVALVGPSGGGKSTVISLIERFYDPMTGNIRIGDTDISLLDLAWYRKKIALVSQEPVLFATTIASNIAYGR-DASQEEIEEAARQANAHDFITTFEDGYKTQVGERGVKLSGGQKQRVAIARALLMDPEILLLDEATSALDAESEHFVKEAIDRAMVNRTVLVIAHRLSTVRSANLVLVMDKGKIVERGNHDSLLAKGGVYRKLVLRQLSASKNER 802 HSP 2 Score: 301.982 bits (772), Expect = 4.555e-86 Identity = 206/593 (34.74%), Postives = 325/593 (54.81%), Query Frame = 2
Query: 2324 LNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGAL--IFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILN------------------DVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
L PE +F+ ++ + + Q + FG ++ + P + + I T L G+ V ++++++ F +G++L R R F A++KQ++A+FD +N G L RLS + I+ ++ ++ I + ++ F +++ V+++ +PI+ VG + +QG + L + +A E I +IRTV + ++E ++ Y EAI + ++ A A G G+ A A G L+ N +M ++ AL + AM ++ +F + A+ MFEL+DRVP I GV+L + I ++V+FSYP R + +L +SF IN +T+ALVGPSG GKST+I L++RFYD + GNI I + I +++ R+ ++VSQEP LF+ +I NIAYG A + ++I AA+ +N HDFI D Y+T+VGE+G SGGQKQR+AIARALL +P ILLLDEATSALD ESE V++A+D+A RT +VIAHRLST+++A+ +LV GKI E G H LL+K G+Y +++ Q
Sbjct: 217 LAYPELGILFIATLALMVSAGSQIAAPYFFGQVISSAIQPGMNALNRTILT----LLGLYVGGSIAAMIRAWLFTLAGQRLVARIRRELFNAIIKQEVAFFD--TNRTGELINRLSSDTQVIQNAVTVNVSMLLRYIVQIIGSLVFMFTLSAKLTGVLISVIPIVGVGAQKYGQFVQGLRKKFQDELGNASTVAEESIGSIRTVRSFSQEKKTMKLYAEAIHESYLVGARLAIASGAFSGVVMAVGQAAIVLVLWYGGKLV--NEGEMNVGILTAFALYTVNVAMAFAFLSSLYGDFMQAVGASFRMFELMDRVPKIKM--EGGVILP--SFKEPIVFDDVRFSYPSREDTEVLKIEEAARQANAHDFITTFEALSFKINPGETVALVGPSGGGKSTVISLIERFYDPMTGNIRIGDTDISLLDLAWYRKKIALVSQEPVLFATTIASNIAYGRDA---SQEEIEEAARQANAHDFITTFEDGYKTQVGERGVKLSGGQKQRVAIARALLMDPEILLLDEATSALDAESEHFVKEAIDRAMVNRTVLVIAHRLSTVRSANLVLVMDKGKIVERGNHDSLLAKGGVYRKLVLRQ 794
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Nematostella
Match: EDO45926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRD5]) HSP 1 Score: 330.872 bits (847), Expect = 1.042e-98 Identity = 196/502 (39.04%), Postives = 292/502 (58.17%), Query Frame = 1
Query: 661 FFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGS----FNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTD--EEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
F + S+ I I N F +++ Q+I +FD +G + ++L D + + + ++ G I F W+L++VT+ PLI + KL+ +A + +A EV+ S++T+ SF E+ E+ RY E +E K+ I+++++ G G + + +YG L+ EG +L G L+ F ++ + A +G EA AA ++F++IDR P I +N +G I FK V F+YPTR D P+L +S ++PGQ VALVG SG GKS+ I L++ Y+ GEVLID +KDL+ +L ++ +V QEPVLF SIK+NI YG D+ D E ++ +ANA +F++ +P DT G++G QLSGGQKQRIAIARAL+RNPKILLLDEATSALD+ESE +VQDA+ K ++G T +I+AHRLST+ A +I I G+V E GT++ L+A +GL+ LV RQ
Sbjct: 75 FRYVASRFNIR-INNLLFGSIVNQDISFFDKTKTGEIVSRLTSDTTKMSDQVSLNVNVFLRNVFKGAGVCIFMFKLSWQLSVVTMIGLPLIAFLSEVYGNYYKKLSTAVQDAIARTNEVAAEVVSSMKTVRSFANEQGELNRYKEKNNEVFKLQIKEAVVIGGYNGCTEIMFLAMEVIILYYGGHLVLEG--YLTGGNLVSF---ILYQLELADALEEIGDVYTGLMEAVGAAEKVFKLIDREPAI--LNNGKVIPLSCEGAIEFKDVSFSYPTRPDIPVLDNVSFMVEPGQVVALVGPSGGGKSTCINLLEHLYEPTGGEVLIDAVPVKDLDHYYLHNKVSLVGQEPVLFARSIKKNIAYGVEDLADNPETVEHVSRLANAHSFVSGMPKGYDTETGEKGLQLSGGQKQRIAIARALIRNPKILLLDEATSALDAESEHLVQDAIYKNLQGHTVLIIAHRLSTIEKADLIIVIDKGQVAEIGTYSHLIAKDGLFNRLVQRQ 568 HSP 2 Score: 276.944 bits (707), Expect = 3.251e-80 Identity = 192/588 (32.65%), Postives = 314/588 (53.40%), Query Frame = 2
Query: 2312 KVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAI-SFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQ------AAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKI----AAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
K++ P+ Y+ L + A Q G ++ +A IE+ ++ + I + + + + F + R ++ F +++ QDI++FD+ G + +RL+ + T++ ++ + + GA V I F WQ+S+V + +P+I + + S + + A+ + ++A E + +++TV + E + YKE ++ K+A G G T+ I Y +L GYL N+ +SF I + L ++ + G + AA +F+L+DR P I +G V+ + + G I+ ++V FSYP RP+I +L++VSF + Q +ALVGPSG GKST I LL+ Y+ G +LID +KD++ L S+V QEP LF+ SI++NIAYG + + ++ +N H F+ +P Y+T+ GEKG SGGQKQRIAIARAL+RNP+ILLLDEATSALD ESE +VQDA+ K +G T ++IAHRLSTI+ AD I+V G++AE GT+ L++K+G++++++ Q
Sbjct: 1 KLLSYLKPDMGYVALAFFFLVAACAAQIFIPYYTGLVIDGIA---IEKNKKKFEMAILTMGLICLGEAICAGFRGAIFRYVASRFNIRINNLLFGSIVNQDISFFDKTKT--GEIVSRLTSDTTKMSDQVSLNVNVFLRNVFK-GAGVCIFMFKLSWQLSVVTMIGLPLIAFLSEVYGNYYKKLSTAVQDAIARTNEVAAEVVSSMKTVRSFANEQGELNRYKEKNNEVFKLQIKEAVVIGGYNGCTEIMFLAMEVIILYYGGHLVLEGYLTGGNL-------VSF---ILYQLELADALEEIGDVYTGLMEAVGAAEKVFKLIDREPAI---LNNGKVIPL-SCEGAIEFKDVSFSYPTRPDIPVLDNVSFMVEPGQVVALVGPSGGGKSTCINLLEHLYEPTGGEVLIDAVPVKDLDHYYLHNKVSLVGQEPVLFARSIKKNIAYGVEDLADNPETVEHVSRLANAHSFVSGMPKGYDTETGEKGLQLSGGQKQRIAIARALIRNPKILLLDEATSALDAESEHLVQDAIYKNLQGHTVLIIAHRLSTIEKADLIIVIDKGQVAEIGTYSHLIAKDGLFNRLVQRQ 568
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Nematostella
Match: EDO39130 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAY5]) HSP 1 Score: 291.582 bits (745), Expect = 8.164e-84 Identity = 178/540 (32.96%), Postives = 291/540 (53.89%), Query Frame = 1
Query: 550 YNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFN----EARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRM--DVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELM-ALNGLYKDLVDRQ 2148
Y + + L+ + Q G+ L ++ V ++ Q + +R F +++RQ +FD +G L N+L D V + D + ++ A G+ + KLT + L I P + + ++ + + +T++ ++ A +AEE + +I + +F E E+ YT+ ++ ++ R+++ G L Y ++ VG++ S ++ T +G + + SF + A++ ++++ DR P + ++ I FK V FTYP+R + I LSL ++ G A+VG SG GKS+L L+ R YD G V + ++K L++ WLR +G V Q+P+LF CSI ENI YG +V+ + I A ANA FI + PN DT+VG+RG LSGGQ+QRIAIARA+++NPKILLLDEATSALDSESE +VQ+AL+++++GRT I +AHRLST++NA I + G + E G++++LM +GL++ LV+RQ
Sbjct: 159 YTASEDPERMMNLLKTICQVLCGVFLLGGAANFGRVYLIQVSGQRIVKQLRQNLFSSILRQETAFFDRTKTGELVNRLSADTILVGKAVTDNVSDGLRAVAQVTAGVSMMAYVSPKLTGIVLLIVPPVAVGAVVYGRFLRSITKRTQDSLADATQVAEERISNIHVVRAFGQENREIKSYTDRVEYVLQLAKREALARAVFFGFTGLSGNL-IVLSVLYSGGMMMTSAQISVGELT----SFMLYTGFVGVSIAGLSSFYSELMKGIGASSRVWQLSDRKPAMSFTGGLQPSIDVLARGIQFKNVNFTYPSRPNARIFADLSLDMRAGSVTAVVGPSGSGKSTLGALLLRLYDPESGSVFVGGHDVKSLSLDWLRGAVGAVHQDPILFSCSIAENIAYGAASSEVSMDAIKDAARQANAANFIESFPNGYDTIVGERGQMLSGGQRQRIAIARAILKNPKILLLDEATSALDSESEHLVQEALERLMKGRTVITIAHRLSTIKNADNIVVLDGGSIVESGSYSQLMEEPDGLFRKLVERQ 693 HSP 2 Score: 270.781 bits (691), Expect = 9.793e-77 Identity = 193/596 (32.38%), Postives = 330/596 (55.37%), Query Frame = 2
Query: 2294 SSAGFFKVMGLNAPEKCYIF----LGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALI-------FGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSK-NGIYSQMISLQS 4045
S ++++ L PE+ I L VS+ AV S + + A +P E+ ++T L G+ ++ ++ + SG+++ K+ R F ++L+Q+ A+FD G L RLS + + +++ + A+ + A V++ +++ +VL VP + VG ++ R ++ + + +L ++ ++A E I NI V +E+ I+ Y + ++ + ++A A+ +G F + ++ L M MT+ IS G L F +++ + KG A++ +++L DR P + SF T G+ ++ ++ I+ +NV F+YP RPN I D+S ++ + A+VGPSG GKST+ LL R YD +G++ + + +K +++ LR V Q+P LFS SI ENIAYG ++ + D I AA+ +N +FI + P+ Y+T VGE+G SGGQ+QRIAIARA+L+NP+ILLLDEATSALD+ESE +VQ+AL++ +GRT + IAHRLSTI+NAD I+V G I ESG++ +L+ + +G++ +++ Q+
Sbjct: 112 SRTDVYRLLNLAKPERWRIAAAIGLLLVSSTVTMAVPFSIGKIIDIIYTASEDP--ERMMNLLKTICQVLCGVFLLGGAANFGRVYLIQVSGQRIVKQLRQNLFSSILRQETAFFDR--TKTGELVNRLSADTILVGKAVTDNVSDGLRAVAQVTAGVSMMAYVSPKLTGIVLLIVPPVAVGAVVYGRFLRSITKRTQDSLADATQVAEERISNIHVVRAFGQENREIKSYTDRVEYVLQLAKREALARAVFFG------FTGLSGNLIVLSVLYSGGM-MMTSAQISVGELTSFMLYTGFVGVSIAGLSSFYSELMKGIGASSRVWQLSDRKPAM-SF-TGGLQPSIDVLARGIQFKNVNFTYPSRPNARIFADLSLDMRAGSVTAVVGPSGSGKSTLGALLLRLYDPESGSVFVGGHDVKSLSLDWLRGAVGAVHQDPILFSCSIAENIAYGAASSEVSMDAIKDAARQANAANFIESFPNGYDTIVGERGQMLSGGQRQRIAIARAILKNPKILLLDEATSALDSESEHLVQEALERLMKGRTVITIAHRLSTIKNADNIVVLDGGSIVESGSYSQLMEEPDGLFRKLVERQT 694
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Medaka
Match: abcb4 (ATP binding cassette subfamily B member 1 [Source:NCBI gene;Acc:101171435]) HSP 1 Score: 553.132 bits (1424), Expect = 7.974e-175 Identity = 290/612 (47.39%), Postives = 394/612 (64.38%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+F +A DI+M++ +M+I G PL+ +VFG+ T + I M+ R K N +L + ++ + + + +Y+ V+ + I + Q+ IR FF +M+Q+IGWFD +G L +L DD+ + GIGDK+G ++Q + FI ++ F +GWKLTLV L++SP + I + SK + T K AY KAG +AEEVL SIRT+ +F G+ E+ RY +NL +A K+G++K+I +G +++ SYAL FWYG++LI G + +G +L VFF ++ +GQ +P++ +F AR AA +++ IID+ P ID +E+G K + +G I F + FTYP+R D IL +SLS++ GQT+ALVG+SGCGKS+ IQL+QRFYD EG V ID +I+ LNV +LRE IGVVSQEP+LF +I ENIRYGR DVT EI+QA ANA FI LPNK +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTT+IVAHRLST+RNA VI G ++GKV E GTH+ELM G+Y+ LV Q
Sbjct: 34 VFRFADGWDILMLVIGTVMAIANGAVLPLMCIVFGDMTDSL--------------------------INGMKGCFRAK---------NATSLFPSFAVYYSIMGAVVLVAAYLQVSLWTIAAGRQVKRIRTLFFHKIMQQDIGWFDINETGELNTRLTDDVYKIQEGIGDKVGMLIQSVSSFITAFVIGFTKGWKLTLVILAVSPALGISGALFSKVLTSFTSKEQSAYAKAGAVAEEVLSSIRTVFAFSGQNKEIERYHKNLQQAKKMGMKKAISANIAMGFTFLMIYFSYALAFWYGSTLILSGE-YTIGSVLTVFFVVIIGVFTMGQTSPNIQTFASARGAAHKVYNIIDKEPIIDSYSEDGFKPDFIKGDIEFSNIHFTYPSRPDVKILENMSLSVKSGQTMALVGSSGCGKSTTIQLLQRFYDPVEGNVSIDGHDIRSLNVRYLREMIGVVSQEPILFATTISENIRYGRPDVTQPEIEQAAKEANAYDFIMNLPNKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQSALDKVRLGRTTLIVAHRLSTIRNADVIAGFQNGKVVEVGTHSELMEKQGVYRMLVTMQ 609 HSP 2 Score: 402.905 bits (1034), Expect = 1.693e-122 Identity = 226/570 (39.65%), Postives = 352/570 (61.75%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFGFLLPAMANP-----NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
+ +G V AI NGAV P I+FG + ++ N + ++ ++ + + + V V++ LQ + + + KR R++ F +++QDI WFD N G L TRL+D+ +I+ G+ +G +I ++ S A I F W+++LV+LA P + + G L +++ F++ ++ A ++G +A E + +IRTV + ++ I+ Y + +++ KKA + G T I+ +YA F L+L + +V F +I G +G++ F + AA ++ ++D+ P I+S+S DG D + GDI+ N+ F+YP RP++ IL ++S ++ S QT+ALVG SGCGKST I LLQRFYD + GN+ ID + I+ +N++ LR++ +VSQEP LF+ +I ENI YG T +I AAK +N +DFI+NLP+++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD ESE IVQ ALDK + GRT +++AHRLSTI+NAD I F++GK+ E GTH EL+ K G+Y ++++Q
Sbjct: 46 LVIGTVMAIANGAVLPLMCIVFGDMTDSLINGMKGCFRAKNATSLFPSFAVYYSIMGAVVLVAAYLQVSLWTIAAGRQVKRIRTLFFHKIMQQDIGWFD--INETGELNTRLTDDVYKIQEGIGDKVGMLIQSVSSFITAFVIGFTKGWKLTLVILAVSPALGISGALFSKVLTSFTSKEQSAYAKAGAVAEEVLSSIRTVFAFSGQNKEIERYHKNLQQAKKMGMKKAISANIAMGFTFLMIYFSYALAFWYGSTLILSGEYTIGSVLTVFFVVIIGVFTMGQTSPNIQTFASARGAAHKVYNIIDKEPIIDSYSEDGFKPDF--IKGDIEFSNIHFTYPSRPDVKILENMSLSVKSGQTMALVGSSGCGKSTTIQLLQRFYDPVEGNVSIDGHDIRSLNVRYLREMIGVVSQEPILFATTISENIRYGRPD--VTQPEIEQAAKEANAYDFIMNLPNKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQSALDKVRLGRTTLIVAHRLSTIRNADVIAGFQNGKVVEVGTHSELMEKQGVYRMLVTMQ 609
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Medaka
Match: abcb4 (ATP binding cassette subfamily B member 1 [Source:NCBI gene;Acc:101171435]) HSP 1 Score: 554.673 bits (1428), Expect = 3.033e-167 Identity = 291/612 (47.55%), Postives = 399/612 (65.20%), Query Frame = 1
Query: 313 LFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+F +A DI+M++ +M+I G PL+ +VFG+ T + P + P++ I N T+ +I M+ + ++ + + + +Y+ V+ + I + Q+ IR FF +M+Q+IGWFD +G L +L DD+ + GIGDK+G ++Q + FI ++ F +GWKLTLV L++SP + I + SK + T K AY KAG +AEEVL SIRT+ +F G+ E+ RY +NL +A K+G++K+I +G +++ SYAL FWYG++LI G + +G +L VFF ++ +GQ +P++ +F AR AA +++ IID+ P ID +E+G K + +G I F + FTYP+R D IL +SLS++ GQT+ALVG+SGCGKS+ IQL+QRFYD EG V ID +I+ LNV +LRE IGVVSQEP+LF +I ENIRYGR DVT EI+QA ANA FI LPNK +TLVGDRGTQ+SGGQKQRIAIARALVRNPKILLLDEATSALD+ESE IVQ ALDKV GRTT+IVAHRLST+RNA VI G ++GKV E GTH+ELM G+Y+ LV Q
Sbjct: 46 VFRFADGWDILMLVIGTVMAIANGAVLPLMCIVFGDMTDSLI---------------NGAPPNMTAGYPNLTFI------NMTI---DIEEEMTVFAVYYSIMGAVVLVAAYLQVSLWTIAAGRQVKRIRTLFFHKIMQQDIGWFDINETGELNTRLTDDVYKIQEGIGDKVGMLIQSVSSFITAFVIGFTKGWKLTLVILAVSPALGISGALFSKVLTSFTSKEQSAYAKAGAVAEEVLSSIRTVFAFSGQNKEIERYHKNLQQAKKMGMKKAISANIAMGFTFLMIYFSYALAFWYGSTLILSGE-YTIGSVLTVFFVVIIGVFTMGQTSPNIQTFASARGAAHKVYNIIDKEPIIDSYSEDGFKPDFIKGDIEFSNIHFTYPSRPDVKILENMSLSVKSGQTMALVGSSGCGKSTTIQLLQRFYDPVEGNVSIDGHDIRSLNVRYLREMIGVVSQEPILFATTISENIRYGRPDVTQPEIEQAAKEANAYDFIMNLPNKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQSALDKVRLGRTTLIVAHRLSTIRNADVIAGFQNGKVVEVGTHSELMEKQGVYRMLVTMQ 632 HSP 2 Score: 533.487 bits (1373), Expect = 5.759e-160 Identity = 272/579 (46.98%), Postives = 396/579 (68.39%), Query Frame = 2
Query: 2306 FFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
FF+V+ LN E +IFLG AI NG +QP F++LF ++ A P+ E + + + I V+FV+ LQ FG SGE LT + R F+AML+QD+ WFD+ N++GALTTRL+ +A Q++G +G L V ++G + ++F + W+++L+VLA VPII V G + ++++ G + DK LE++GKIA E IENIRTV++LTRE F Y+E +K KKAH GF + +Q+ I+ AYA F +L++ + VF+ A++FGAM +G++ + APN+ K K++AAH+ L+++VP I++ S +G D +G++ ++V+F+YP RP++ IL ++ + +T+ALVG SGCGKST I LL+RFYDT G +++D+ IK +NI LR IVSQEP LF ++ ENIAYGD+++ T ++I AAAKA+NIH FI LP +Y T+ G+KG+ SGGQKQRIAIARA+LRNP++LLLDEATSALDTESEK+VQDALD+A +GRTC+++AHRLSTI+NAD+I VF+ G + E GTH +LL+K G+Y +++ Q
Sbjct: 704 FFRVLMLNKSEWPFIFLGTFCAIINGGIQPVFAVLFSKIIAVFAEPDQEVVRERSQFFSLMFVTIGCVSFVTMFLQGFCFGKSGEVLTLKLRLGAFKAMLRQDLGWFDDSKNSVGALTTRLATDAAQVQGATGARLATVAQNFANLGTGIILAFIYGWELTLLVLAVVPIIAVAGAVGMQMLTGHAAEDKKELEKAGKIATEAIENIRTVASLTREAKFESLYQENLKVPYKNSQKKAHTHGFTFSFSQSMIYFAYAGCFRFGAWLIVQGRMDVEGVFLVISAVLFGAMAVGEANSFAPNYAKAKMSAAHLMMLINKVPDIDNLSNEGETPD--KFNGNVIFKDVRFNYPSRPDVPILKGLNLKVKKGETLALVGSSGCGKSTTIQLLERFYDTKEGKVVLDDVDIKQLNIHWLRSQIGIVSQEPVLFDCTLAENIAYGDNSRTVTMEEIEAAAKAANIHKFIEELPQRYNTQAGDKGTQLSGGQKQRIAIARAILRNPQVLLLDEATSALDTESEKVVQDALDQASKGRTCIIVAHRLSTIRNADRIAVFQGGVVVEQGTHQQLLAKKGVYHMLVTTQ 1280 HSP 3 Score: 420.239 bits (1079), Expect = 1.253e-121 Identity = 239/548 (43.61%), Postives = 343/548 (62.59%), Query Frame = 1
Query: 517 PDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
PD +E+VR + S F+LM F I F+ ++ F + + +R F+A++RQ++GWFD ++NS GALT +L D V G ++ + Q +A GIILAF+ GW+LTL+ L++ P+I + + + + + + KAG IA E + +IRT+ S E + Y ENL K +K+ G +++ +YA F +GA LI +G + G + +V + L +G+A ++ +A+ +AA + +I++VP+ID ++ EG+ + F G + FK V+F YP+R D PIL GL+L ++ G+T+ALVG+SGCGKS+ IQL++RFYD+ EG+V++DD +IK LN+ WLR QIG+VSQEPVLF C++ ENI YG VT EEI+ A AN FI LP + +T GD+GTQLSGGQKQRIAIARA++RNP++LLLDEATSALD+ESEK+VQDALD+ +GRT IIVAHRLST+RNA I + G V EQGTH +L+A G+Y LV Q
Sbjct: 749 PD-QEVVRER--------SQFFSLM------FVTIGCVSFVTMFLQGFCFGKSGEVLTLKLRLGAFKAMLRQDLGWFDDSKNSVGALTTRLATDAAQVQGATGARLATVAQNFANLGTGIILAFIYGWELTLLVLAVVPIIAVAGAVGMQMLTGHAAEDKKELEKAGKIATEAIENIRTVASLTREAKFESLYQENLKVPYKNSQKKAHTHGFTFSFSQSMIYFAYAGCFRFGAWLIVQGRMDVEG-VFLVISAVLFGAMAVGEANSFAPNYAKAKMSAAHLMMLINKVPDIDNLSNEGETPDKFNGNVIFKDVRFNYPSRPDVPILKGLNLKVKKGETLALVGSSGCGKSTTIQLLERFYDTKEGKVVLDDVDIKQLNIHWLRSQIGIVSQEPVLFDCTLAENIAYGDNSRTVTMEEIEAAAKAANIHKFIEELPQRYNTQAGDKGTQLSGGQKQRIAIARAILRNPQVLLLDEATSALDTESEKVVQDALDQASKGRTCIIVAHRLSTIRNADRIAVFQGGVVVEQGTHQQLLAKKGVYHMLVTTQ 1280 HSP 4 Score: 402.905 bits (1034), Expect = 6.826e-116 Identity = 232/588 (39.46%), Postives = 354/588 (60.20%), Query Frame = 2
Query: 2348 IFLGCVSAIFNGAVQPSFSILFGFLLPAMAN----------PNI----------EQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 4051
+ +G V AI NGAV P I+FG + ++ N PN+ E+ + Y I A + V A++ L + G KR R++ F +++QDI WFD N G L TRL+D+ +I+ G+ +G +I ++ S A I F W+++LV+LA P + + G L +++ F++ ++ A ++G +A E + +IRTV + ++ I+ Y + +++ KKA + G T I+ +YA F L+L + +V F +I G +G++ F + AA ++ ++D+ P I+S+S DG D + GDI+ N+ F+YP RP++ IL ++S ++ S QT+ALVG SGCGKST I LLQRFYD + GN+ ID + I+ +N++ LR++ +VSQEP LF+ +I ENI YG T +I AAK +N +DFI+NLP+++ET VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD ESE IVQ ALDK + GRT +++AHRLSTI+NAD I F++GK+ E GTH EL+ K G+Y ++++Q+ +
Sbjct: 58 LVIGTVMAIANGAVLPLMCIVFGDMTDSLINGAPPNMTAGYPNLTFINMTIDIEEEMTVFAVYYSIMGAVVLVAAYLQVSLWTIAAGRQ----VKRIRTLFFHKIMQQDIGWFD--INETGELNTRLTDDVYKIQEGIGDKVGMLIQSVSSFITAFVIGFTKGWKLTLVILAVSPALGISGALFSKVLTSFTSKEQSAYAKAGAVAEEVLSSIRTVFAFSGQNKEIERYHKNLQQAKKMGMKKAISANIAMGFTFLMIYFSYALAFWYGSTLILSGEYTIGSVLTVFFVVIIGVFTMGQTSPNIQTFASARGAAHKVYNIIDKEPIIDSYSEDGFKPDF--IKGDIEFSNIHFTYPSRPDVKILENMSLSVKSGQTMALVGSSGCGKSTTIQLLQRFYDPVEGNVSIDGHDIRSLNVRYLREMIGVVSQEPILFATTISENIRYGRPD--VTQPEIEQAAKEANAYDFIMNLPNKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQSALDKVRLGRTTLIVAHRLSTIRNADVIAGFQNGKVVEVGTHSELMEKQGVYRMLVTMQTFQ 635
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Medaka
Match: abcb11a (ATP binding cassette subfamily B member 11 [Source:NCBI gene;Acc:101157171]) HSP 1 Score: 514.227 bits (1323), Expect = 3.977e-153 Identity = 264/580 (45.52%), Postives = 389/580 (67.07%), Query Frame = 2
Query: 2297 SAGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMIS 4036
SA +++ N PE Y+ LG + A NG+V P +++LF +L + P++++Q I + +AV++F S +Q F SGE LT+R R + F+AMLKQ+I WFD+P N+ GALTTRL+ +A+ ++G +G +G +I ++ S+GA+ I+F F W+++LV+L F+P+I + G+ Q +++ GF+ DK A+E +G+++ E + NIRTV+ LT+E +F++ ++E ++ K+A+ G +GLTQ IF AYAA+F GYL+ + VF A++ LG++ + P++ K K AAA F+LLDRVP I+ TDG EN G+++ N KF+YP RP+ +L + ++ QT+ALVG SGCGKST + LL+RFYD G +LID +++ LR IVSQEP LF SI ENI YGD++ ++I+ AAK +N+HDF++ LPD+Y+T+VG +GS S GQKQRIAIARA++RNP+ILLLDEATSALDTESE+IVQ ALD+A++GRTC+VIAHRLSTIQNAD I V G + E GTH EL++K G Y ++++
Sbjct: 725 SASVARILKYNQPEWPYMLLGSLGAAINGSVNPIYAVLFSQILGTFSIPDLDEQRRQINGICLLFCVVAVISFFSQFIQGFSFAKSGELLTRRLRKVGFQAMLKQEIGWFDDPENSPGALTTRLATDASMVQGATGSQIGMIINSLTSIGASFIIAFYFSWKLTLVILGFLPLIGLSGVFQAKMLTGFAKEDKKAMEAAGRVSSEALGNIRTVAGLTKERSFVESFEEKLELPYKSAKKRANIYGLCFGLTQCVIFMAYAASFRFGGYLVRAEGLQYMLVFRVISAVVISGTALGRASSFTPDYAKAKTAAAQFFKLLDRVPKIS--HTDGE--KWENFKGEVEFLNCKFTYPTRPDTQVLKGLVVSVKPGQTLALVGSSGCGKSTGVQLLERFYDPDEGKVLIDGRPSYSVSVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSHRINMEEIVEAAKTANLHDFVMTLPDKYDTQVGAQGSQLSRGQKQRIAIARAIIRNPKILLLDEATSALDTESEQIVQSALDEARKGRTCIVIAHRLSTIQNADIIAVMSHGVVIEQGTHDELMAKRGAYYKLVT 1300 HSP 2 Score: 472.241 bits (1214), Expect = 7.346e-139 Identity = 274/616 (44.48%), Postives = 377/616 (61.20%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G +L+ +AT D VMM+ ++ G PL++LV+G T Y+ + Q L N+ + T I NI M+ + + GI + ++SY + F+V + Q IR +FR VM+ IGWFD + G L ++ DDIN + I D++ ++ + FI G ++ F+ GWKLTLV +++SPLI + +++ + +LT + +AY KAG +A+EVL SIRT+ +F GE+ E RY NL EA G++K + G G + ++F YAL FWYG+ L+ + G+++ VFF L+A T +GQA P + +F R+AA +FE IDR PEID ++E+G KL+ +G I F V F YP+R D IL L+L I+ G+T A VG SG GK+S +QL+QRFYD EG V +D +++ LN+ WLR IG+V QEPVLF +I ENIRYGR VT E+I QA ANA FI LP K DTLVG+ G Q+SGGQKQRIAIARALVR P+ILLLD ATSALD+ESE VQ+AL+KV RTTI VAHRLST+R+A VI G + G+ E+GTH EL+ G+Y LV Q
Sbjct: 29 GYFQLYRFATGRDTVMMVVGGFCALIHGAATPLMLLVYGMMTDTFVAYELEV----QELKDPNKECLNDTIYWINGSIYETTDNQTVTCGVNIEGQMTQFAYYYIGIGSGVLLVSYFQIMFWVSAAARQTQRIRKTYFRRVMQMEIGWFDCNSVGELNTRISDDINKISNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVVAVSPLIGMGAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEEKEAERYDRNLVEAQNWGVKKGSIIGVFQGYLWCIIFLCYALAFWYGSKLVIDSKELSPGNLIQVFFGVLIAATNLGQAAPCLEAFASGRAAAKTVFETIDREPEIDCLSEDGYKLDKIKGDIEFHNVTFYYPSRPDIKILDDLNLHIKAGETTAFVGPSGSGKTSAVQLIQRFYDPKEGMVTLDGHDLRSLNIQWLRSLIGIVEQEPVLFATTIAENIRYGRPGVTMEDIIQATREANAYHFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRKPRILLLDMATSALDNESEATVQEALNKVHTERTTISVAHRLSTIRSADVIVGFEHGRAVEKGTHRELLERKGVYFTLVTLQ 640 HSP 3 Score: 382.874 bits (982), Expect = 3.741e-109 Identity = 243/588 (41.33%), Postives = 356/588 (60.54%), Query Frame = 1
Query: 391 WPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIR-EGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
WP ++L S A +N + L SQ I + SIPD++E R ++G+ F +A+ F ++ F + + +R F+A+++Q IGWFD +NS GALT +L D + V G +IG ++ I+AF WKLTLV L PLI + + +K + ++ +A AG ++ E LG+IRT+ E+ V + E L+ K +++ + G GL ++F +YA F +G L+R EG +++ + V + +++ T +G+A+ + +A++AAA+ F+++DRVP+I + +G+K +F+G++ F KFTYPTR D +L GL +S++PGQT+ALVG+SGCGKS+ +QL++RFYD +EG+VLID ++V +LR QIG+VSQEPVLF CSI ENI+YG + EEI +A AN F+ LP+K DT VG +G+QLS GQKQRIAIARA++RNPKILLLDEATSALD+ESE+IVQ ALD+ +GRT I++AHRLST++NA +I + G V EQGTH ELMA G Y LV
Sbjct: 739 WPYMLL-----GSLGAAINGSVNPIYAVLFSQ---ILGTFSIPDLDEQRRQ---------------INGICLLFCVVAVISFFSQFIQGFSFAKSGELLTRRLRKVGFQAMLKQEIGWFDDPENSPGALTTRLATDASMVQGATGSQIGMIINSLTSIGASFIIAFYFSWKLTLVILGFLPLIGLSGVFQAKMLTGFAKEDKKAMEAAGRVSSEALGNIRTVAGLTKERSFVESFEEKLELPYKSAKKRANIYGLCFGLTQCVIFMAYAASFRFGGYLVRAEGLQYML--VFRVISAVVISGTALGRASSFTPDYAKAKTAAAQFFKLLDRVPKIS--HTDGEKWENFKGEVEFLNCKFTYPTRPDTQVLKGLVVSVKPGQTLALVGSSGCGKSTGVQLLERFYDPDEGKVLIDGRPSYSVSVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSHRINMEEIVEAAKTANLHDFVMTLPDKYDTQVGAQGSQLSRGQKQRIAIARAIIRNPKILLLDEATSALDTESEQIVQSALDEARKGRTCIVIAHRLSTIQNADIIAVMSHGVVIEQGTHDELMAKRGAYYKLV 1299 HSP 4 Score: 320.857 bits (821), Expect = 7.990e-89 Identity = 208/537 (38.73%), Postives = 302/537 (56.24%), Query Frame = 2
Query: 2444 NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL--YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
NIE Q Y I GI + S Q + + ++ + T+R R F +++ +I WFD N++G L TR+SD+ +I + + I I + + F W+++LVV+A P+I +G L + + + A ++G +A E + +IRTV+ E + Y + + KK G G IF YA F L++ +S + + FG LI A NLG++ F G+ AA +FE +DR P I+ S DG LD + GDI+ NV F YP RP+I IL+D++ +I + +T A VGPSG GK++ + L+QRFYD G + +D + ++ +NIQ LR L IV QEP LF+ +I ENI YG D II A + +N + FI++LP +++T VGE G SGGQKQRIAIARAL+R PRILLLD ATSALD ESE VQ+AL+K RT + +AHRLSTI++AD I+ F+ G+ E GTH ELL + G+Y +++LQ+
Sbjct: 116 NIEGQMTQFAYYYI---GIGSGVLLVSYFQIMFWVSAAARQTQRIRKTYFRRVMQMEIGWFD--CNSVGELNTRISDDINKISNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVVAVSPLIGMGAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEEKEAERYDRNLVEAQNWGVKKGSIIGVFQGYLWCIIFLCYALAFWYGSKLVIDSKELSPGNLIQVFFGVLI-AATNLGQAAPCLEAFASGRAAAKTVFETIDREPEIDCLSEDGYKLD--KIKGDIEFHNVTFYYPSRPDIKILDDLNLHIKAGETTAFVGPSGSGKTSAVQLIQRFYDPKEGMVTLDGHDLRSLNIQWLRSLIGIVEQEPVLFATTIAENIRYGRPGVTMED--IIQATREANAYHFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRKPRILLLDMATSALDNESEATVQEALNKVHTERTTISVAHRLSTIRSADVIVGFEHGRAVEKGTHRELLERKGVYFTLVTLQNQ 642
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Medaka
Match: ABCB4 (multidrug resistance protein 1-like [Source:NCBI gene;Acc:105357817]) HSP 1 Score: 462.225 bits (1188), Expect = 1.080e-144 Identity = 234/486 (48.15%), Postives = 344/486 (70.78%), Query Frame = 2
Query: 2585 ITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
I F+++ QD+ WFD+ N++GALTTRL+ +A Q++G +G L V ++G + ++F + W+++L+VLA VPII V G + ++++ G + DK LE++GKIA E IENIRTV++LTRE F Y+E +K KKAH GF + +Q+ I+ AYA F +L++ + VF+ A++FGAM +G++ + APN+ K K++AAH+ L+++VP I++ S +G D +G++ ++V+F+YP RP++ IL ++ + +T+ALVG SGCGKST I LL+RFYDT G +++D+ IK +NI LR IVSQEP LF ++ ENIAYGD+++ T ++I AAAKA+NIH FI LP +Y T+ G+KG+ SGGQKQRIAIARA+LRNP++LLLDEATSALDTESEK+VQDALD+A +GRTC+++AHRLSTI+NAD+I VF+ G + E GTH +LL+K G+Y +++ Q
Sbjct: 20 IIFDSL--QDLGWFDDSKNSVGALTTRLATDAAQVQGATGARLATVAQNFANLGTGIILAFIYGWELTLLVLAVVPIIAVAGAVGMQMLTGHAAEDKKELEKAGKIATEAIENIRTVASLTREAKFESLYQENLKVPYKNSQKKAHTHGFTFSFSQSMIYFAYAGCFRFGAWLIVQGRMDVEGVFLVISAVLFGAMAVGEANSFAPNYAKAKMSAAHLMMLINKVPDIDNLSNEGETPD--KFNGNVIFKDVRFNYPSRPDVPILKGLNLKVKKGETLALVGSSGCGKSTTIQLLERFYDTKEGKVVLDDVDIKQLNIHWLRSQIGIVSQEPVLFDCTLAENIAYGDNSRTVTMEEIEAAAKAANIHKFIEELPQRYNTQAGDKGTQLSGGQKQRIAIARAILRNPQVLLLDEATSALDTESEKVVQDALDQASKGRTCIIVAHRLSTIRNADRIAVFQGGVVVEQGTHQQLLAKKGVYHMLVTTQ 501 HSP 2 Score: 406.371 bits (1043), Expect = 3.336e-125 Identity = 222/477 (46.54%), Postives = 313/477 (65.62%), Query Frame = 1
Query: 730 QNIGWFD-AQNS-GALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
Q++GWFD ++NS GALT +L D V G ++ + Q +A GIILAF+ GW+LTL+ L++ P+I + + + + + + KAG IA E + +IRT+ S E + Y ENL K +K+ G +++ +YA F +GA LI +G + G + +V + L +G+A ++ +A+ +AA + +I++VP+ID ++ EG+ + F G + FK V+F YP+R D PIL GL+L ++ G+T+ALVG+SGCGKS+ IQL++RFYD+ EG+V++DD +IK LN+ WLR QIG+VSQEPVLF C++ ENI YG VT EEI+ A AN FI LP + +T GD+GTQLSGGQKQRIAIARA++RNP++LLLDEATSALD+ESEK+VQDALD+ +GRT IIVAHRLST+RNA I + G V EQGTH +L+A G+Y LV Q
Sbjct: 26 QDLGWFDDSKNSVGALTTRLATDAAQVQGATGARLATVAQNFANLGTGIILAFIYGWELTLLVLAVVPIIAVAGAVGMQMLTGHAAEDKKELEKAGKIATEAIENIRTVASLTREAKFESLYQENLKVPYKNSQKKAHTHGFTFSFSQSMIYFAYAGCFRFGAWLIVQGRMDVEG-VFLVISAVLFGAMAVGEANSFAPNYAKAKMSAAHLMMLINKVPDIDNLSNEGETPDKFNGNVIFKDVRFNYPSRPDVPILKGLNLKVKKGETLALVGSSGCGKSTTIQLLERFYDTKEGKVVLDDVDIKQLNIHWLRSQIGIVSQEPVLFDCTLAENIAYGDNSRTVTMEEIEAAAKAANIHKFIEELPQRYNTQAGDKGTQLSGGQKQRIAIARAILRNPQVLLLDEATSALDTESEKVVQDALDQASKGRTCIIVAHRLSTIRNADRIAVFQGGVVVEQGTHQQLLAKKGVYHMLVTTQ 501
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Medaka
Match: abcb11b (bile salt export pump [Source:NCBI gene;Acc:101163554]) HSP 1 Score: 481.485 bits (1238), Expect = 1.708e-141 Identity = 283/637 (44.43%), Postives = 387/637 (60.75%), Query Frame = 1
Query: 301 GVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSS-------TSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMS--------------QQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
G +LF +ATC D++MM+ + ++ G PL++LVFG T Y + L+ + E + ++ T DM + + S+ +FT +S MS + GI +F++ Y ++ +V + QI IR +F VMR IGWFD + G L +L DDIN ++ I D++ +Q + F+ G + FV+GWKLTLV ++ SPLI I +++ + KLT +AY KAG +A+EVL SIRT+ +F GE EV RY NL A + GIRK ++ G G + ++F YAL FWYG++L+ + + G +L VFF L+A +GQA+P + +F R AA IFE IDR P+ID ++E G KL +G I F V F YP+R + IL LS+ ++ G+T A VG SG GKS+ IQL+QRFYD EG V +D +I+ LN+ WLR IG+V QEPVLF +I ENIRYGR V+ ++I A ANA FI LP K DTLVG+ G Q+SGGQKQRIAIARALVRNP+ILLLD ATSALD+ESE +VQ+ALDKV GRTTI +AHRLST++NA VI G + G+ E+G H EL+ G+Y LV Q
Sbjct: 56 GFFQLFRFATCKDVLMMVLGSVCAVLHGSAQPLMLLVFGLLTDTFIDYDIELQELSD---DRKECVNNTIQWKRNYTGTLDMTLPLNQSFGSLINSTLEMFTPLSNMSCGILDIEHEMTLFALYYVGIGAGVFLLGYFQISLWVTAAARQIQLIRKLYFTKVMRMEIGWFDCTSVGELNTRLSDDINKINDAIADQVSIFVQRFTTFVCGFCIGFVKGWKLTLVIVAASPLIGIGAGLMALFVAKLTGMELQAYAKAGAVADEVLSSIRTVAAFGGEIKEVERYDRNLVSAQRWGIRKGLIMGFFTGYMWLIIFLCYALAFWYGSTLVVDTAEYTPGTLLQVFFGVLVAAMSLGQASPCLEAFAAGRGAATIIFETIDREPQIDCLSESGYKLERVKGDIEFHNVTFHYPSRPEVKILDQLSVQVKSGETTAFVGPSGAGKSTAIQLIQRFYDPLEGMVTLDGHDIRGLNIQWLRSLIGIVEQEPVLFATTIAENIRYGRPGVSMDDIINAAKEANAYNFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKVRMGRTTISIAHRLSTIKNADVIVGFEHGRAVERGKHNELLERKGVYFTLVTLQ 689 HSP 2 Score: 476.478 bits (1225), Expect = 7.327e-140 Identity = 251/575 (43.65%), Postives = 374/575 (65.04%), Query Frame = 2
Query: 2312 KVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMIS 4036
+++ NAPE Y+ G + A NG V P +S+LF +L + + Q +I + A + VV+F + +LQ F SGE LT+R R I F AML Q+I WFD+ N+ GALTTRL+ +A+Q++G +G +G ++ ++ ++G AV +SF F W+++L++L F+P + + G Q +++ GF+ DK A+E +G+I+ E + NIRT++ L +E F++ Y+ + KKA+ G YG Q +F +A++ GYL+ + VF A++ LG++ + P++ K KI+AA F+LLDR+P I+ +S G + N G+I+ + KF+YP RP+I +LN ++ ++ QT+A VG SGCGKST + LL+RFYD +G +LID + K +N+ LR IVSQEP LF SI ENI YGD+++ + D++I AAK + +HDF+ LP+QY T VG +GS S GQKQRIAIARA++R+P+ILLLDEATSALDTESEK VQ+ALDKA+EGRTC+VIAHRLSTIQN+D I V G + E G+H +L+ G Y ++++
Sbjct: 785 RILKYNAPEWPYMLFGSLGAAINGGVNPVYSLLFSQILATFSVQDPAAQRREIDGICVFFAMVGVVSFFTQMLQGYAFSKSGELLTRRLRRIGFHAMLGQEIGWFDDHRNSPGALTTRLATDASQVQGATGSQIGMIVNSLTNIGVAVLMSFYFSWKLTLLILCFLPFLALSGGFQAKMLTGFAKQDKQAMETAGRISGEALNNIRTIAGLGKEKNFVEMYEFQLDAPYQAALKKANVYGACYGFAQCVVFMTNSASYRFGGYLVKQEGLHFSLVFRVISAIVTSGTALGRASSYTPDYAKAKISAARFFQLLDRIPTISVYSDKGDKWN--NFQGNIEFIDCKFTYPTRPDIQVLNGLTVSVKPGQTLAFVGSSGCGKSTSVQLLERFYDPDHGKVLIDGHDSKHVNVPYLRSKIGIVSQEPILFDCSIAENIKYGDNSREISMDEVILAAKKAQLHDFVTALPEQYNTNVGSQGSQLSRGQKQRIAIARAIIRDPKILLLDEATSALDTESEKTVQEALDKAREGRTCIVIAHRLSTIQNSDIIAVMSRGILIEQGSHDQLMGLKGAYYKLVT 1357 HSP 3 Score: 360.533 bits (924), Expect = 1.557e-101 Identity = 210/521 (40.31%), Postives = 319/521 (61.23%), Query Frame = 1
Query: 589 MSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQ--NSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYG--RMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLV 2139
+ G+ FA + + F + F + + +R F A++ Q IGWFD + GALT +L D + V G +IG ++ ++++F WKLTL+ L P + + +K + ++ +A AG I+ E L +IRTI EK V Y LD + ++K+ + G G ++F + + + +G L+++ G + V + + + T +G+A+ + + +A+ +AA F+++DR+P I +++GDK N+FQG I F KFTYPTR D +L GL++S++PGQT+A VG+SGCGKS+ +QL++RFYD + G+VLID + K +NV +LR +IG+VSQEP+LF CSI ENI+YG +++ +E+ A A F+ ALP + +T VG +G+QLS GQKQRIAIARA++R+PKILLLDEATSALD+ESEK VQ+ALDK EGRT I++AHRLST++N+ +I + G + EQG+H +LM L G Y LV
Sbjct: 837 IDGICVFFAMVGVVSFFTQMLQGYAFSKSGELLTRRLRRIGFHAMLGQEIGWFDDHRNSPGALTTRLATDASQVQGATGSQIGMIVNSLTNIGVAVLMSFYFSWKLTLLILCFLPFLALSGGFQAKMLTGFAKQDKQAMETAGRISGEALNNIRTIAGLGKEKNFVEMYEFQLDAPYQAALKKANVYGACYGFAQCVVFMTNSASYRFGGYLVKQ-EGLHFSLVFRVISAIVTSGTALGRASSYTPDYAKAKISAARFFQLLDRIPTISVYSDKGDKWNNFQGNIEFIDCKFTYPTRPDIQVLNGLTVSVKPGQTLAFVGSSGCGKSTSVQLLERFYDPDHGKVLIDGHDSKHVNVPYLRSKIGIVSQEPILFDCSIAENIKYGDNSREISMDEVILAAKKAQLHDFVTALPEQYNTNVGSQGSQLSRGQKQRIAIARAIIRDPKILLLDEATSALDTESEKTVQEALDKAREGRTCIVIAHRLSTIQNSDIIAVMSRGILIEQGSHDQLMGLKGAYYKLV 1356 HSP 4 Score: 320.857 bits (821), Expect = 1.212e-88 Identity = 209/532 (39.29%), Postives = 300/532 (56.39%), Query Frame = 2
Query: 2465 DIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSH 4048
++ + + GI F+ Q + + + + R + F +++ +I WFD ++G L TRLSD+ +I + + + + I F W+++LV++A P+I +G L + + + A ++G +A E + +IRTV+ E ++ Y + +K GF G IF YA F L++ Y + VF FG L+ AM+LG++ F G+ AA +FE +DR P I+ S G L E + GDI+ NV F YP RP + IL+ +S + S +T A VGPSG GKST I L+QRFYD L G + +D + I+ +NIQ LR L IV QEP LF+ +I ENI YG + D II AAK +N ++FI++LP +++T VGE G SGGQKQRIAIARAL+RNPRILLLD ATSALD ESE +VQ+ALDK + GRT + IAHRLSTI+NAD I+ F+ G+ E G H ELL + G+Y +++LQS
Sbjct: 169 EMTLFALYYVGIGAGVFLLGYFQISLWVTAAARQIQLIRKLYFTKVMRMEIGWFD--CTSVGELNTRLSDDINKINDAIADQVSIFVQRFTTFVCGFCIGFVKGWKLTLVIVAASPLIGIGAGLMALFVAKLTGMELQAYAKAGAVADEVLSSIRTVAAFGGEIKEVERYDRNLVSAQRWGIRKGLIMGFFTGYMWLIIFLCYALAFWYGSTLVVDTAEYTPGTLLQVF--FGVLV-AAMSLGQASPCLEAFAAGRGAATIIFETIDREPQIDCLSESGYKL--ERVKGDIEFHNVTFHYPSRPEVKILDQLSVQVKSGETTAFVGPSGAGKSTAIQLIQRFYDPLEGMVTLDGHDIRGLNIQWLRSLIGIVEQEPVLFATTIAENIRYGRPG--VSMDDIINAAKEANAYNFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKVRMGRTTISIAHRLSTIKNADVIVGFEHGRAVERGKHNELLERKGVYFTLVTLQSQ 691
BLAST of Multidrug resistance protein 1-like protein vs. Planmine SMEST
Match: SMESG000004996.1 (SMESG000004996.1) HSP 1 Score: 1287.32 bits (3330), Expect = 0.000e+0 Identity = 685/685 (100.00%), Postives = 685/685 (100.00%), Query Frame = 1
Query: 97 MTISKVKVSNDETEKTDIPTRENAIYRAYDLENPGFERSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXGVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQK 2151
MTISKVKVSNDETEKTDIPTRENAIYRAYDLENPGFERSDSVHSKISKHSIEKIKKSKNKKKAEIKSVGVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICMLMLSKTMMKLTQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSILTGTLLGLVMGLMFGSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQK
Sbjct: 1 MTISKVKVSNDETEKTDIPTRENAIYRAYDLENPGFERSDSVHSKISKHSIEKIKKSKNKKKAEIKSVGVGKLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICMLMLSKTMMKLTQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSILTGTLLGLVMGLMFGSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQK 685 HSP 2 Score: 1218.37 bits (3151), Expect = 0.000e+0 Identity = 634/635 (99.84%), Postives = 635/635 (100.00%), Query Frame = 2
Query: 2147 KKIDHGQKGEKEEIYVNVDEEIIKESPTKRLSXXXXXXXXXXXXXXXXXSSAGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 4051
+KIDHGQKGEKEEIYVNVDEEIIKESPTKRLSVAKIEEVIEENKEIVKKSSAGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTLNGNILIDNNKIKDINIQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK
Sbjct: 684 QKIDHGQKGEKEEIYVNVDEEIIKESPTKRLSVAKIEEVIEENKEIVKKSSAGFFKVMGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLYNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTLNGNILIDNNKIKDINIQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 1318 HSP 3 Score: 355.525 bits (911), Expect = 3.731e-100 Identity = 206/522 (39.46%), Postives = 312/522 (59.77%), Query Frame = 1
Query: 610 FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLN--DFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQKNR 2157
AGIA+ F+ S + F + + R+ F A+++Q+I WFD + N GALT +L D+ + G+ +G ++ + ++F W+++LV L+ P+I + ++ + M + A ++G IA E + +IRT+ + E + Y E + + +K+ G L GL +F +YA F L+ N + ++ I F + + +G++ +F + + AAA +FE++DRVP I+ + +G L+ + G I + VKF+YP R + IL +S +I QT+ALVG SGCGKS++I L+QRFYD+ G +LID+ IKD+N+ LR+ +VSQEP LF SI+ENI YG TD++I A +N FI LP++ +T VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD+ESEKIVQDALDK EGRT +++AHRLST++NA I KDGK+ E GTH EL++ NG+Y ++ Q ++
Sbjct: 798 LAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLY-NMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTLNGNILIDNNKIKDINIQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQSHK 1318 HSP 4 Score: 348.591 bits (893), Expect = 6.279e-98 Identity = 206/519 (39.69%), Postives = 310/519 (59.73%), Query Frame = 2
Query: 2489 LAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLLY-NMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDKAQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
AGIA+ F+ S + F + + R+ F A+++Q+I WFD + N GALT +L D+ + G+ +G ++ + ++F W+++LV L+ P+I + ++ + M + A ++G IA E + +IRT+ + E + Y E + + +K+ G L GL +F +YA F L+ N + ++ I F + + +G++ +F + + AAA +FE++DRVP I+ + +G L+ + G I + VKF+YP R + IL +S +I QT+ALVG SGCGKS++I L+QRFYD+ G +LID+ IKD+N+ LR+ +VSQEP LF SI+ENI YG TD++I A +N FI LP++ +T VG++G+ SGGQKQRIAIARAL+RNP+ILLLDEATSALD+ESEKIVQDALDK EGRT +++AHRLST++NA I KDGK+ E GTH EL++ NG+Y ++ Q
Sbjct: 172 FAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFD--AQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICMLMLSKTMMKLTQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSILTGTLLGLVMGLMFGSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLN--DFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMD--VTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVVEGRTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 684
BLAST of Multidrug resistance protein 1-like protein vs. Planmine SMEST
Match: SMESG000030111.1 (SMESG000030111.1) HSP 1 Score: 672.929 bits (1735), Expect = 0.000e+0 Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = -1
Query: 6413 MNVKKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLESICEVLHLRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDELVHWYEGXXXXXXXXXXXXXXYKNDSVYQIVVKLLPISKRKLDPEKKPLFYENDSLAKSLIXXXXXXXXXXXXXXXNFQTNKRIHGCDNEDSIEGDKLESMFAIPDSFVSRLHWVTMLPVTVLCFLTIPDCRRPGIYKKLFFLTFLMSLVWITICIYLLVWMICITG 7510
MNVKKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLESICEVLHLRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDELVHWYEGLIMLIIYSLYILLLYKNDSVYQIVVKLLPISKRKLDPEKKPLFYENDSLAKSLIDSENDDDLEKEDLNSNFQTNKRIHGCDNEDSIEGDKLESMFAIPDSFVSRLHWVTMLPVTVLCFLTIPDCRRPGIYKKLFFLTFLMSLVWITICIYLLVWMICITG
Sbjct: 1 MNVKKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLESICEVLHLRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDELVHWYEGLIMLIIYSLYILLLYKNDSVYQIVVKLLPISKRKLDPEKKPLFYENDSLAKSLIDSENDDDLEKEDLNSNFQTNKRIHGCDNEDSIEGDKLESMFAIPDSFVSRLHWVTMLPVTVLCFLTIPDCRRPGIYKKLFFLTFLMSLVWITICIYLLVWMICITG 366
BLAST of Multidrug resistance protein 1-like protein vs. Planmine SMEST
Match: SMESG000030111.1 (SMESG000030111.1) HSP 1 Score: 408.683 bits (1049), Expect = 9.688e-131 Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = -1
Query: 6905 MNVKKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLESICEVLHLRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDELVH 7510
MNVKKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLESICEVLHLRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDELVH
Sbjct: 1 MNVKKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSNCTKRSVEEFPDDFMTRVDKKNGGVLFHIVMSAYLFMAIAIVCDDYFVSSLESICEVLHLRHDVAGATFMALGGSAPELCTAVIAVFITDSDVGVSTIIGSALFNVLCVTAICGIFAGKVIQLTWWPVFRDCMIYCITIITLTIVLNDELVH 202
BLAST of Multidrug resistance protein 1-like protein vs. Planmine SMEST
Match: SMESG000027395.1 (SMESG000027395.1) HSP 1 Score: 421.394 bits (1082), Expect = 5.474e-129 Identity = 247/615 (40.16%), Postives = 358/615 (58.21%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALD-KVVEG-RTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQ 2148
+LF + DI+++ +I SIG+GV PL +L+F + + + +N F ++ PI FA +A I S+V +T + Q ++ +F A++RQ I WFD Q +G + + DDI+++ I DK+ Q +Q + F II+A GWKLTLV P++II + + K AY AGGIAEE+L S+RT+V+F ++ EV RY++NL +A +GI+KSI+ G G V +F +L F+ G LI N + G ++ VF + ++ + IG A P + +F A++AA IFE+IDRVP ID + G + F I F+ V F Y TR IL + +I G+TVA +G SG GKS++I L+QRFYD GEV++ D N+KD+++ W R IGVV QEP LF SI+ENI+ G++D T++EI ++ +NA FI A DT +GDRGT SGGQKQRIAIA AL+R+P++LLLDEATSALD++SEK+VQ LD + EG RT ++VAHRL TVRNA +I +K+GK+ EQG H+ L+A +GLY +++ Q
Sbjct: 54 RLFRFMNSMDILLISLTVISSIGIGVALPLNMLIFKDVVNLLVLPTGNIN----FSSAIRNPI-----------------------------------NYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDKQQTGQMITRFSDDIDSIEVAISDKLAQFIQQLSLFFSSIIIAATSGWKLTLVGCMAIPIMIIILYSNLHFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYVGLLLINSEN-YDPGTVIQVFLTIVLGSISIGYAAPLIENFMTAQAAAYSIFEVIDRVPSIDH-RKLGIIPDTFAKDIKFQNVTFQYSTRPGMTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEVILGDQNVKDIDLNWYRSHIGVVQQEPPLFSGSIRENIQMGKLDATEDEIIKSTQYSNAYDFIMATKETFDTKLGDRGTGFSGGQKQRIAIATALIRDPQLLLLDEATSALDNKSEKLVQKTLDTTIAEGKRTIVMVAHRLQTVRNADIIVVMKNGKIVEQGNHSSLLAQHGLYWEMLGTQ 627 HSP 2 Score: 325.479 bits (833), Expect = 8.223e-96 Identity = 209/581 (35.97%), Postives = 340/581 (58.52%), Query Frame = 2
Query: 2324 LNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANP--NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDK--AQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
+N+ + I L +S+I G P ++F ++ + P NI SA IR + A +A A ++S +Q++ +G++ ++R +S F A+L+Q+I+WFD+ G + TR SD+ I + L I + +++ I+ W+++LV +PI+ + + ++ + + A +G IA E + ++RTV + + + Y + + K KK+ QG G ++F + F + G LL+ Y+ + VF++ ++ G++++G + + NF + AA +FE++DRVP I+ G++ D + DIK +NV F Y RP + IL++ +F I +T+A +GPSG GKSTII LLQRFYD L+G +++ + +KDI++ R +V QEP LFS SIRENI G T+D+II + + SN +DFI+ + ++TK+G++G+ FSGGQKQRIAIA AL+R+P++LLLDEATSALD +SEK+VQ LD A+ RT V++AHRL T++NAD I+V K+GKI E G H LL+++G+Y +M+ Q
Sbjct: 59 MNSMDILLISLTVISSIGIGVALPLNMLIFKDVVNLLVLPTGNINFSSA-IRNPINYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDK--QQTGQMITRFSDDIDSIEVAISDKLAQFIQQLSLFFSSIIIAATSGWKLTLVGCMAIPIMIIILYSNLHFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYV-GLLLINSENYDPGTVIQVFLT---IVLGSISIGYAAPLIENFMTAQAAAYSIFEVIDRVPSIDHRKL-GIIPD--TFAKDIKFQNVTFQYSTRPGMTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEVILGDQNVKDIDLNWYRSHIGVVQQEPPLFSGSIRENIQMGKLDA--TEDEIIKSTQYSNAYDFIMATKETFDTKLGDRGTGFSGGQKQRIAIATALIRDPQLLLLDEATSALDNKSEKLVQKTLDTTIAEGKRTIVMVAHRLQTVRNADIIVVMKNGKIVEQGNHSSLLAQHGLYWEMLGTQ 627
BLAST of Multidrug resistance protein 1-like protein vs. Planmine SMEST
Match: SMESG000027395.1 (SMESG000027395.1) HSP 1 Score: 421.779 bits (1083), Expect = 2.376e-127 Identity = 247/616 (40.10%), Postives = 359/616 (58.28%), Query Frame = 1
Query: 310 KLFSYATCSDIVMMICAIIMSIGMGVTWPLIILVFGNTTSQMATYQYYMNSLTQFLTSQNEPITSSTSIPDMEEIVRNKKYNFTLSSSNIFTLMSGMSQQFAGIALAMFIMSYVSVTFFVITSQHQISAIRNAFFRAVMRQNIGWFDAQNSGALTNKLVDDINTVHAGIGDKIGQMMQWYACFIGGIILAFVRGWKLTLVTLSISPLIIICXXXXXXXXXXXXQKASEAYGKAGGIAEEVLGSIRTIVSFRGEKYEVARYTENLDEACKIGIRKSIXXXXXXXXXXXXXXXSYALGFWYGASLIREGNGFLVGDMLIVFFSCLMATTGIGQATPHMGSFNEARSAAAEIFEIIDRVPEIDKMNEEGDKLNDFQGKITFKYVKFTYPTRTDCPILTGLSLSIQPGQTVALVGASGCGKSSLIQLMQRFYDSNEGEVLIDDTNIKDLNVMWLREQIGVVSQEPVLFGCSIKENIRYGRMDVTDEEIDQALVMANAKTFINALPNKLDTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEKIVQDALDKVV-EG-RTTIIVAHRLSTVRNAHVIFGIKDGKVQEQGTHAELMALNGLYKDLVDRQK 2151
+LF + DI+++ +I SIG+GV PL +L+F + + + +N F ++ PI FA +A I S+V +T + Q ++ +F A++RQ I WFD Q +G + + DDI+++ I DK+ Q +Q + F II+A GWKLTLV P++II + + K AY AGGIAEE+L S+RT+V+F ++ EV RY++NL +A +GI+KSI+ G G V +F +L F+ G LI N + G ++ VF + ++ + IG A P + +F A++AA IFE+IDRVP ID + G + F I F+ V F Y TR IL + +I G+TVA +G SG GKS++I L+QRFYD GEV++ D N+KD+++ W R IGVV QEP LF SI+ENI+ G++D T++EI ++ +NA FI A DT +GDRGT SGGQKQRIAIA AL+R+P++LLLDEATSALD++SEK+VQ LD + EG RT ++VAHRL TVRNA +I +K+GK+ EQG H+ L+A +GLY +++ Q+
Sbjct: 54 RLFRFMNSMDILLISLTVISSIGIGVALPLNMLIFKDVVNLLVLPTGNIN----FSSAIRNPI-----------------------------------NYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDKQQTGQMITRFSDDIDSIEVAISDKLAQFIQQLSLFFSSIIIAATSGWKLTLVGCMAIPIMIIILYSNLHFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYVGLLLINSEN-YDPGTVIQVFLTIVLGSISIGYAAPLIENFMTAQAAAYSIFEVIDRVPSIDH-RKLGIIPDTFAKDIKFQNVTFQYSTRPGMTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEVILGDQNVKDIDLNWYRSHIGVVQQEPPLFSGSIRENIQMGKLDATEDEIIKSTQYSNAYDFIMATKETFDTKLGDRGTGFSGGQKQRIAIATALIRDPQLLLLDEATSALDNKSEKLVQKTLDTTIAEGKRTIVMVAHRLQTVRNADIIVVMKNGKIVEQGNHSSLLAQHGLYWEMLGTQE 628 HSP 2 Score: 325.094 bits (832), Expect = 3.146e-94 Identity = 209/581 (35.97%), Postives = 340/581 (58.52%), Query Frame = 2
Query: 2324 LNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANP--NIEQQSADIRTYVIALAGIAVVAFVSSVLQNVGFGNSGEQLTKRCRSITFEAMLKQDIAWFDEPSNNIGALTTRLSDEATQIRGVSGESLGAVITAICSMGAAVAISFGFCWQISLVVLAFVPIIFVGGILQIRIMQGFSNSDKGALEESGKIAVECIENIRTVSNLTREHTFIQHYKEAIKKLDGEVSKKAHAQGFLYGLTQAAIFCAYAATFMLAGYLLL----YNMSKMTNVFISFGALIFGAMNLGKSMAMAPNFGKGKIAAAHMFELLDRVPPINSFSTDGVVLDVENMSGDIKIENVKFSYPLRPNIIILNDVSFNINSNQTIALVGPSGCGKSTIIGLLQRFYDTXXXXXXXXXXXXXXXXXQNLRQLFSIVSQEPTLFSYSIRENIAYGDSAKIFTDDQIIAAAKASNIHDFIVNLPDQYETKVGEKGSNFSGGQKQRIAIARALLRNPRILLLDEATSALDTESEKIVQDALDK--AQEGRTCVVIAHRLSTIQNADKILVFKDGKIAESGTHFELLSKNGIYSQMISLQ 4042
+N+ + I L +S+I G P ++F ++ + P NI SA IR + A +A A ++S +Q++ +G++ ++R +S F A+L+Q+I+WFD+ G + TR SD+ I + L I + +++ I+ W+++LV +PI+ + + ++ + + A +G IA E + ++RTV + + + Y + + K KK+ QG G ++F + F + G LL+ Y+ + VF++ ++ G++++G + + NF + AA +FE++DRVP I+ G++ D + DIK +NV F Y RP + IL++ +F I +T+A +GPSG GKSTII LLQRFYD L+G +++ + +KDI++ R +V QEP LFS SIRENI G T+D+II + + SN +DFI+ + ++TK+G++G+ FSGGQKQRIAIA AL+R+P++LLLDEATSALD +SEK+VQ LD A+ RT V++AHRL T++NAD I+V K+GKI E G H LL+++G+Y +M+ Q
Sbjct: 59 MNSMDILLISLTVISSIGIGVALPLNMLIFKDVVNLLVLPTGNINFSSA-IRNPINYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDK--QQTGQMITRFSDDIDSIEVAISDKLAQFIQQLSLFFSSIIIAATSGWKLTLVGCMAIPIMIIILYSNLHFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYV-GLLLINSENYDPGTVIQVFLT---IVLGSISIGYAAPLIENFMTAQAAAYSIFEVIDRVPSIDHRKL-GIIPD--TFAKDIKFQNVTFQYSTRPGMTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEVILGDQNVKDIDLNWYRSHIGVVQQEPPLFSGSIRENIQMGKLDA--TEDEIIKSTQYSNAYDFIMATKETFDTKLGDRGTGFSGGQKQRIAIATALIRDPQLLLLDEATSALDNKSEKLVQKTLDTTIAEGKRTIVMVAHRLQTVRNADIIVVMKNGKIVEQGNHSSLLAQHGLYWEMLGTQ 627 The following BLAST results are available for this feature:
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Multidrug resistance protein 1-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021731 ID=SMED30021731|Name=Multidrug resistance protein 1-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=7813bpback to top protein sequence of SMED30021731-orf-1 >SMED30021731-orf-1 ID=SMED30021731-orf-1|Name=SMED30021731-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=579bp MGLNAPEKCYIFLGCVSAIFNGAVQPSFSILFGFLLPAMANPNIEQQSADback to top protein sequence of SMED30021731-orf-2 >SMED30021731-orf-2 ID=SMED30021731-orf-2|Name=SMED30021731-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=368bp MNVKKHKSFLKIILCYFSLIFGSIFFLKVNDIGGKDANFDGKSRKLLSLSback to top protein sequence of SMED30021731-orf-3 >SMED30021731-orf-3 ID=SMED30021731-orf-3|Name=SMED30021731-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=147bp MELILVDDTTKSTGFVNINECEYLHIENHKNPFFALNLLKLLSVLFVEIFback to top protein sequence of SMED30021731-orf-4 >SMED30021731-orf-4 ID=SMED30021731-orf-4|Name=SMED30021731-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=700bp MTISKVKVSNDETEKTDIPTRENAIYRAYDLENPGFERSDSVHSKISKHSback to top Annotated Terms
The following terms have been associated with this transcript:
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