Cystinosin

Overview
NameCystinosin
Smed IDSMED30021721
Length (bp)285
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cystinosin (SMED30021721) t-SNE clustered cells

Violin plots show distribution of expression levels for Cystinosin (SMED30021721) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cystinosin (SMED30021721) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cystinosin (SMED30021721) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021721

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30021721h1SMcG0012444 dd_Smed_v4_4571_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021721h1SMcG0012444 dd_Smed_v4_4571_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cystinosin vs. Ensembl Human
Match: CTNS (cystinosin, lysosomal cystine transporter [Source:HGNC Symbol;Acc:HGNC:2518])

HSP 1 Score: 55.0694 bits (131), Expect = 1.037e-9
Identity = 27/52 (51.92%), Postives = 32/52 (61.54%), Query Frame = 1 
Query:  130 HVVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
             V+GL+FDF+  N  GF +YSVFNIGL W+  IK  F    P   NPV  ND
Sbjct:  154 SVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSND 205
[Close]
BLAST of Cystinosin vs. Ensembl Human
Match: CTNS (cystinosin, lysosomal cystine transporter [Source:HGNC Symbol;Acc:HGNC:2518])

HSP 1 Score: 54.299 bits (129), Expect = 1.310e-9
Identity = 27/52 (51.92%), Postives = 32/52 (61.54%), Query Frame = 1 
Query:  130 HVVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
             V+GL+FDF+  N  GF +YSVFNIGL W+  IK  F    P   NPV  ND
Sbjct:    7 SVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSND 58
[Close]
BLAST of Cystinosin vs. Ensembl Human
Match: CTNS (cystinosin, lysosomal cystine transporter [Source:HGNC Symbol;Acc:HGNC:2518])

HSP 1 Score: 54.299 bits (129), Expect = 1.464e-9
Identity = 27/51 (52.94%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GL+FDF+  N  GF +YSVFNIGL W+  IK  F    P   NPV  ND
Sbjct:  155 VIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSND 205
[Close]
BLAST of Cystinosin vs. Ensembl Human
Match: CTNS (cystinosin, lysosomal cystine transporter [Source:HGNC Symbol;Acc:HGNC:2518])

HSP 1 Score: 54.299 bits (129), Expect = 1.464e-9
Identity = 27/51 (52.94%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GL+FDF+  N  GF +YSVFNIGL W+  IK  F    P   NPV  ND
Sbjct:  155 VIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSND 205
[Close]
BLAST of Cystinosin vs. Ensembl Human
Match: CTNS (cystinosin, lysosomal cystine transporter [Source:HGNC Symbol;Acc:HGNC:2518])

HSP 1 Score: 53.5286 bits (127), Expect = 2.195e-9
Identity = 27/51 (52.94%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GL+FDF+  N  GF +YSVFNIGL W+  IK  F    P   NPV  ND
Sbjct:    8 VIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSND 58
[Close]
BLAST of Cystinosin vs. Ensembl Celegans
Match: ctns-1 (Cystinosin homolog [Source:UniProtKB/Swiss-Prot;Acc:Q09500])

HSP 1 Score: 53.1434 bits (126), Expect = 1.886e-9
Identity = 25/51 (49.02%), Postives = 35/51 (68.63%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGLNFDFL  N +GF +Y++FN+ +++   +KN +   NPR   PV LND
Sbjct:  157 VVGLNFDFLSLNLVGFCAYAIFNLLMYYNSHVKNEYNIVNPRSPPPVLLND 207
[Close]
BLAST of Cystinosin vs. Ensembl Celegans
Match: ctns-1 (Cystinosin homolog [Source:UniProtKB/Swiss-Prot;Acc:Q09500])

HSP 1 Score: 52.7582 bits (125), Expect = 2.378e-9
Identity = 25/51 (49.02%), Postives = 35/51 (68.63%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGLNFDFL  N +GF +Y++FN+ +++   +KN +   NPR   PV LND
Sbjct:  157 VVGLNFDFLSLNLVGFCAYAIFNLLMYYNSHVKNEYNIVNPRSPPPVLLND 207
[Close]
BLAST of Cystinosin vs. Ensembl Fly
Match: CG17119 (gene:FBgn0039045 transcript:FBtr0084357)

HSP 1 Score: 53.5286 bits (127), Expect = 1.730e-9
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V GLNFDFL  N +GF  YS+FN GL++I+ ++N ++   P   NPV LND
Sbjct:  160 VEGLNFDFLALNIVGFTLYSMFNCGLYFIEDLQNEYEVRYPLGVNPVMLND 210
[Close]
BLAST of Cystinosin vs. Ensembl Fly
Match: CG17119 (gene:FBgn0039045 transcript:FBtr0344299)

HSP 1 Score: 53.5286 bits (127), Expect = 1.730e-9
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V GLNFDFL  N +GF  YS+FN GL++I+ ++N ++   P   NPV LND
Sbjct:  160 VEGLNFDFLALNIVGFTLYSMFNCGLYFIEDLQNEYEVRYPLGVNPVMLND 210
[Close]
BLAST of Cystinosin vs. Ensembl Zebrafish
Match: ctns (cystinosin, lysosomal cystine transporter [Source:ZFIN;Acc:ZDB-GENE-050522-352])

HSP 1 Score: 57.3806 bits (137), Expect = 7.946e-11
Identity = 28/51 (54.90%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGLNFDFL  N  GF +YSVFN+GLFW+  I+  F   +P    PV  ND
Sbjct:  160 VVGLNFDFLALNLTGFIAYSVFNVGLFWVTYIQEEFLKKDPNGVIPVDAND 210
[Close]
BLAST of Cystinosin vs. Ensembl Xenopus
Match: klhl24 (kelch like family member 24 [Source:Xenbase;Acc:XB-GENE-1000313])

HSP 1 Score: 51.2174 bits (121), Expect = 1.684e-8
Identity = 26/51 (50.98%), Postives = 31/51 (60.78%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGL+FDFL  N  G  +Y VFN+GLFWI  +K  F    P    PV+ ND
Sbjct:  256 VVGLSFDFLALNLTGHIAYGVFNLGLFWIPYVKEQFLELYPNGVFPVEAND 306
[Close]
BLAST of Cystinosin vs. Ensembl Mouse
Match: Ctns (cystinosis, nephropathic [Source:MGI Symbol;Acc:MGI:1932872])

HSP 1 Score: 54.299 bits (129), Expect = 1.210e-9
Identity = 27/51 (52.94%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GL+FDFL  N  GF +YSVFNIGL W+  I+  F    P   NPV  ND
Sbjct:  155 VIGLSFDFLALNLTGFVAYSVFNIGLLWVPYIQEEFLLKYPNGVNPVDSND 205
[Close]
BLAST of Cystinosin vs. Ensembl Mouse
Match: Ctns (cystinosis, nephropathic [Source:MGI Symbol;Acc:MGI:1932872])

HSP 1 Score: 54.299 bits (129), Expect = 1.210e-9
Identity = 27/51 (52.94%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GL+FDFL  N  GF +YSVFNIGL W+  I+  F    P   NPV  ND
Sbjct:  155 VIGLSFDFLALNLTGFVAYSVFNIGLLWVPYIQEEFLLKYPNGVNPVDSND 205
[Close]
BLAST of Cystinosin vs. UniProt/SwissProt
Match: sp|A7MB63|CTNS_BOVIN (Cystinosin OS=Bos taurus OX=9913 GN=CTNS PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.652e-11
Identity = 30/51 (58.82%), Postives = 36/51 (70.59%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGL+FDF++ N +GF +YSVFNIGLFW+ SIK  F    P   NPV  ND
Sbjct:  155 VVGLSFDFVVLNLMGFVAYSVFNIGLFWVPSIKEQFLLKYPNGVNPVDSND 205
[Close]
BLAST of Cystinosin vs. UniProt/SwissProt
Match: sp|A8WN56|CTNS_CAEBR (Cystinosin homolog OS=Caenorhabditis briggsae OX=6238 GN=ctns-1 PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 5.185e-9
Identity = 25/51 (49.02%), Postives = 36/51 (70.59%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGLNFDFL  N +GF +Y++FN+ +++   +KN++   NPR   PV LND
Sbjct:  156 VVGLNFDFLSLNLVGFGAYAMFNLLMYYNSHVKNIYSMENPRSPPPVLLND 206
[Close]
BLAST of Cystinosin vs. UniProt/SwissProt
Match: sp|O60931|CTNS_HUMAN (Cystinosin OS=Homo sapiens OX=9606 GN=CTNS PE=1 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 7.032e-9
Identity = 27/51 (52.94%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GL+FDF+  N  GF +YSVFNIGL W+  IK  F    P   NPV  ND
Sbjct:  155 VIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSND 205
[Close]
BLAST of Cystinosin vs. UniProt/SwissProt
Match: sp|P57757|CTNS_MOUSE (Cystinosin OS=Mus musculus OX=10090 GN=Ctns PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 8.467e-9
Identity = 27/51 (52.94%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GL+FDFL  N  GF +YSVFNIGL W+  I+  F    P   NPV  ND
Sbjct:  155 VIGLSFDFLALNLTGFVAYSVFNIGLLWVPYIQEEFLLKYPNGVNPVDSND 205
[Close]
BLAST of Cystinosin vs. UniProt/SwissProt
Match: sp|Q9VCR7|CTNS_DROME (Cystinosin homolog OS=Drosophila melanogaster OX=7227 GN=CG17119 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 1.734e-8
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V GLNFDFL  N +GF  YS+FN GL++I+ ++N ++   P   NPV LND
Sbjct:  160 VEGLNFDFLALNIVGFTLYSMFNCGLYFIEDLQNEYEVRYPLGVNPVMLND 210
[Close]
BLAST of Cystinosin vs. TrEMBL
Match: V3ZZQ3 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_219532 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.430e-13
Identity = 36/67 (53.73%), Postives = 48/67 (71.64%), Query Frame = 1 
Query:   85 VTFRNVIFNAYPFNLHVVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+F   +F+ Y F   VVGLNFD++ YN +GF +Y  FN+GL+WI S+KN +K  +PR  NPVQLND
Sbjct:   38 VSFYPQVFSNY-FRKSVVGLNFDYIAYNLLGFLAYGFFNVGLYWIGSVKNEYKRQHPRGINPVQLND 103
[Close]
BLAST of Cystinosin vs. TrEMBL
Match: W4YIC8 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 6.714e-12
Identity = 30/52 (57.69%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:  130 HVVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
             V+GLNFDFL YN  GF +Y VFN+G++WI+ IK+ +K  NP   NPV LND
Sbjct:  152 SVIGLNFDFLAYNITGFVAYGVFNVGMYWIEPIKDAYKAKNPYGVNPVLLND 203
[Close]
BLAST of Cystinosin vs. TrEMBL
Match: A0A210R5V9 (Cystinosin OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT08272 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 8.351e-12
Identity = 32/51 (62.75%), Postives = 40/51 (78.43%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GLNFDFL YN  GF +YS FN+GL+WI SI++ +K  +PR  NPVQLND
Sbjct:  164 VIGLNFDFLAYNITGFIAYSFFNVGLYWIKSIEDDYKSLHPRGINPVQLND 214
[Close]
BLAST of Cystinosin vs. TrEMBL
Match: A0A1S3IZU2 (cystinosin OS=Lingula unguis OX=7574 GN=LOC106168859 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.139e-11
Identity = 32/51 (62.75%), Postives = 39/51 (76.47%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GLNFDFL YN  GF +Y VFN+G+FWI SI+  +K  +PR  NPVQLND
Sbjct:  174 VIGLNFDFLSYNITGFVAYGVFNVGMFWIPSIEAEYKDLHPRGVNPVQLND 224
[Close]
BLAST of Cystinosin vs. TrEMBL
Match: A0A0D2WRH9 (Cystinosin-like protein OS=Capsaspora owczarzaki (strain ATCC 30864) OX=595528 GN=CAOG_004575 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.623e-11
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGLNFDFL YN  GF  YS FNIGLFW+ SI++L+  ++P   NPVQ ND
Sbjct:  155 VVGLNFDFLAYNITGFLCYSAFNIGLFWVTSIQDLYFADHPDGVNPVQAND 205
[Close]
BLAST of Cystinosin vs. Ensembl Cavefish
Match: ctns (cystinosin, lysosomal cystine transporter [Source:NCBI gene;Acc:103043999])

HSP 1 Score: 63.1586 bits (152), Expect = 6.698e-13
Identity = 32/52 (61.54%), Postives = 34/52 (65.38%), Query Frame = 1 
Query:  130 HVVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
             VVGLNFDFL  N+ GF +YSVFNIGLFWI  IK  F    P   NPV  ND
Sbjct:  159 SVVGLNFDFLALNFTGFIAYSVFNIGLFWIPYIKEEFIQTEPNGVNPVDAND 210
[Close]
BLAST of Cystinosin vs. Ensembl Cavefish
Match: ctns (cystinosin, lysosomal cystine transporter [Source:NCBI gene;Acc:103043999])

HSP 1 Score: 63.1586 bits (152), Expect = 6.698e-13
Identity = 32/52 (61.54%), Postives = 34/52 (65.38%), Query Frame = 1 
Query:  130 HVVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
             VVGLNFDFL  N+ GF +YSVFNIGLFWI  IK  F    P   NPV  ND
Sbjct:  159 SVVGLNFDFLALNFTGFIAYSVFNIGLFWIPYIKEEFIQTEPNGVNPVDAND 210
[Close]
BLAST of Cystinosin vs. Ensembl Nematostella
Match: EDO46894 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNQ1])

HSP 1 Score: 60.077 bits (144), Expect = 1.860e-12
Identity = 26/51 (50.98%), Postives = 36/51 (70.59%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GLNFDF+ YN  GF +Y +FNIG+FWI  ++  ++  +P   NPVQ ND
Sbjct:   50 VIGLNFDFVCYNLTGFVAYGLFNIGMFWIPLVQQQYEAKHPNGVNPVQTND 100
[Close]
BLAST of Cystinosin vs. Ensembl Medaka
Match: ctns (cystinosin, lysosomal cystine transporter [Source:NCBI gene;Acc:101155673])

HSP 1 Score: 64.3142 bits (155), Expect = 2.634e-13
Identity = 30/51 (58.82%), Postives = 34/51 (66.67%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGLNFDFL  N  GF +YSVFNIGLFW+  ++  F   NP   NPV  ND
Sbjct:  284 VVGLNFDFLALNLTGFIAYSVFNIGLFWVPYVQEEFLKTNPNGINPVNSND 334
[Close]
BLAST of Cystinosin vs. Planmine SMEST
Match: SMESG000079054.1 (SMESG000079054.1)

HSP 1 Score: 107.842 bits (268), Expect = 1.538e-29
Identity = 51/51 (100.00%), Postives = 51/51 (100.00%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND
Sbjct:  120 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 170
[Close]
BLAST of Cystinosin vs. Planmine SMEST
Match: SMESG000016911.1 (SMESG000016911.1)

HSP 1 Score: 46.2098 bits (108), Expect = 4.042e-7
Identity = 25/51 (49.02%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  133 VVGLNFDFLIYNWIGFFSYSVFNIGLFWIDSIKNLFKYNNPRVANPVQLND 285
            V+GLNFDFL YN  GF +YS+FN  L++  +++  F        NPVQLND
Sbjct:   38 VIGLNFDFLGYNITGFIAYSIFNSALYFNKNLQQEFYKTYKGKFNPVQLND 88
The following BLAST results are available for this feature:
BLAST of Cystinosin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CTNS1.037e-951.92cystinosin, lysosomal cystine transporter [Source:... [more]
CTNS1.310e-951.92cystinosin, lysosomal cystine transporter [Source:... [more]
CTNS1.464e-952.94cystinosin, lysosomal cystine transporter [Source:... [more]
CTNS1.464e-952.94cystinosin, lysosomal cystine transporter [Source:... [more]
CTNS2.195e-952.94cystinosin, lysosomal cystine transporter [Source:... [more]
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BLAST of Cystinosin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ctns-11.886e-949.02Cystinosin homolog [Source:UniProtKB/Swiss-Prot;A... [more]
ctns-12.378e-949.02Cystinosin homolog [Source:UniProtKB/Swiss-Prot;A... [more]
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BLAST of Cystinosin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG171191.730e-950.98gene:FBgn0039045 transcript:FBtr0084357[more]
CG171191.730e-950.98gene:FBgn0039045 transcript:FBtr0344299[more]
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BLAST of Cystinosin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ctns7.946e-1154.90cystinosin, lysosomal cystine transporter [Source:... [more]
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BLAST of Cystinosin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
klhl241.684e-850.98kelch like family member 24 [Source:Xenbase;Acc:XB... [more]
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BLAST of Cystinosin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Ctns1.210e-952.94cystinosis, nephropathic [Source:MGI Symbol;Acc:MG... [more]
Ctns1.210e-952.94cystinosis, nephropathic [Source:MGI Symbol;Acc:MG... [more]
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BLAST of Cystinosin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A7MB63|CTNS_BOVIN1.652e-1158.82Cystinosin OS=Bos taurus OX=9913 GN=CTNS PE=2 SV=1[more]
sp|A8WN56|CTNS_CAEBR5.185e-949.02Cystinosin homolog OS=Caenorhabditis briggsae OX=6... [more]
sp|O60931|CTNS_HUMAN7.032e-952.94Cystinosin OS=Homo sapiens OX=9606 GN=CTNS PE=1 SV... [more]
sp|P57757|CTNS_MOUSE8.467e-952.94Cystinosin OS=Mus musculus OX=10090 GN=Ctns PE=1 S... [more]
sp|Q9VCR7|CTNS_DROME1.734e-850.98Cystinosin homolog OS=Drosophila melanogaster OX=7... [more]
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BLAST of Cystinosin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V3ZZQ32.430e-1353.73Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
W4YIC86.714e-1257.69Uncharacterized protein OS=Strongylocentrotus purp... [more]
A0A210R5V98.351e-1262.75Cystinosin OS=Mizuhopecten yessoensis OX=6573 GN=K... [more]
A0A1S3IZU21.139e-1162.75cystinosin OS=Lingula unguis OX=7574 GN=LOC1061688... [more]
A0A0D2WRH91.623e-1162.75Cystinosin-like protein OS=Capsaspora owczarzaki (... [more]
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BLAST of Cystinosin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ctns6.698e-1361.54cystinosin, lysosomal cystine transporter [Source:... [more]
ctns6.698e-1361.54cystinosin, lysosomal cystine transporter [Source:... [more]
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BLAST of Cystinosin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cystinosin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cystinosin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO468941.860e-1250.98Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cystinosin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ctns2.634e-1358.82cystinosin, lysosomal cystine transporter [Source:... [more]
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BLAST of Cystinosin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000079054.11.538e-29100.00SMESG000079054.1[more]
SMESG000016911.14.042e-749.02SMESG000016911.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021721 ID=SMED30021721|Name=Cystinosin|organism=Schmidtea mediterranea sexual|type=transcript|length=285bp
ATGAAGGTTGATGTACAGATTGTACAAAAAATGATGCTTTCAAAAATGCC
TGAATGTTCAGAATATGAGACGGAAACCGAATATGTGACTTTTAGAAACG
TAATATTTAATGCTTATCCATTCAATTTACATGTTGTTGGATTGAATTTT
GATTTCTTAATTTACAATTGGATTGGATTTTTTTCATATAGTGTTTTCAA
TATTGGATTATTTTGGATAGATTCGATTAAGAACTTGTTTAAATATAATA
ATCCAAGAGTTGCTAATCCAGTGCAACTGAATGAC
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR005282LC_transporter
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005282Lysosomal cystine transporterPANTHERPTHR13131CYSTINOSINcoord: 44..95
NoneNo IPR availablePANTHERPTHR13131:SF5CYSTINOSINcoord: 44..95
NoneNo IPR availableTMHMMTMhelixcoord: 52..74