SMED30021707

Overview
NameSMED30021707
Smed IDSMED30021707
Length (bp)1227
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30021707 (SMED30021707) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021707 (SMED30021707) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021707 (SMED30021707) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021707 (SMED30021707) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021707

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30021707SMESG000063964.1 dd_Smed_v4_11231_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021707SMESG000063964.1 dd_Smed_v4_11231_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021707SMESG000063964.1 dd_Smed_v4_11231_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
anterior sideSMED30021707SMESG000063964.1 dd_Smed_v4_11231_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30021707SMESG000063964.1 dd_Smed_v4_11231_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021707SMESG000063964.1 dd_Smed_v4_11231_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30021707SMESG000063964.1 dd_Smed_v6_11231_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30021707SMESG000063964.1 OX_Smed_1.0.11804ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30021707 vs. TrEMBL
Match: A0A4E0RIR9 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_000341 PE=4 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 7.238e-31
Identity = 79/239 (33.05%), Postives = 123/239 (51.46%), Query Frame = 2 
Query:  326 FCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTC----DLHFIR-------------EHSETD---SYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVILQIKDEQP-NMIDQEN 979
            FC++ VLL+SMQ+ LG + +GLG+YLR   P L  ++C++WA LP+  +G FG YFC       K   ++  ++ +   +S+++  L L ASI SG  G  I+ + + C     L+ I              +  +TD    +  +CC D             M++ +C C    F   + L +   Y  + CRY+FS++KDY ILQS+LM V TGVC+W  ++  +  QP    D EN
Sbjct:  537 FCQLIVLLVSMQISLGLAATGLGLYLRWRVPLLPLEECSFWAALPITITGLFGTYFCFQRRISRKNPQITLAIRRIAGALSLISCILCLVASIFSGQKGSLIATYGTDCIPSNPLYSIHNSSIKNNDNSNNNQEKKTDYDLGFEQQCCIDGVDR---------MSEPLCQC----FNVRNQLMW--SYRGIACRYLFSSVKDYLILQSALMAVGTGVCLWFWVVLTEQFQPVKRTDNEN 760
[Close]
BLAST of SMED30021707 vs. TrEMBL
Match: A0A504YEC9 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_08303 PE=4 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 5.938e-29
Identity = 76/239 (31.80%), Postives = 119/239 (49.79%), Query Frame = 2 
Query:  326 FCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTC-------DLH-------------FIREHSETDSYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVILQIKDEQP-NMIDQEN 979
            FC++ VLL+SMQ+ LG + +GLG+YLR   P L  ++C++WA LP+  +G  G YFC       K   ++  ++ +   +S+++  L L ASI SG  G  I+ + + C        +H               R+      +  +CC D             M++ +C C    F   + L +   Y  + CRY+FS++KDY ILQS+LM V TGVC+W  ++  +  QP    D EN
Sbjct:  538 FCQLIVLLVSMQISLGLAATGLGLYLRWRVPLLPLEECSFWAALPISITGFIGTYFCFQRRISRKNPQITLAIRRIAGVLSLISCILCLVASIFSGQKGSLIATYGTDCIPSNPLYSVHNSSIKNNNNSNNSLERKPDYDLGFEQQCCIDGVDR---------MSEPLCQC----FNVRNQLMW--SYRGIACRYLFSSVKDYLILQSALMAVGTGVCLWFWVVLTEQFQPVKRTDNEN 761
[Close]
BLAST of SMED30021707 vs. TrEMBL
Match: A0A419Q0G8 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_3134s PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 8.623e-24
Identity = 76/258 (29.46%), Postives = 126/258 (48.84%), Query Frame = 2 
Query:  194 DVFRSPNNNFKNTESYLSISRNFSIVSGRRLSSMEQNKEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDH-EIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSETDS---------YPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL 937
            D   +PN +FK   S + I        G  +   +  +    + FC+++VLL+ MQ+ LG S + LG YL    P L  ++C +WA +PL  +G  G  FC  +  +++ RS+ S  ++     +++++  L L ASI SG  G  I+     C +  +R  ++T +         Y  +CC D  +            D   VC+     N  +  + +    +TCRY+ S++KDY ILQS+LM VA GVC+W  ++
Sbjct:  428 DPTLAPNTSFK--PSGIDIEHGSPPSVGSPICPPDSQETARGLCFCQLHVLLVIMQIVLGMSTTALGFYLHWKIPLLPLEECAFWAAIPLSFTGIIGSLFCFENRCQLFNRSY-SVSMRRATGILTLISCVLCLVASIFSGQKGNVIASSGHPCQI--VRPTTQTKNATQSSGLPEYEHQCCSDTSSL-----------DGHNVCQCLNMRNQVIWSYHK----ITCRYLISSVKDYLILQSALMAVAAGVCLWFWVV 665
[Close]
BLAST of SMED30021707 vs. TrEMBL
Match: A0A4S2MH70 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000928 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 5.519e-22
Identity = 71/214 (33.18%), Postives = 110/214 (51.40%), Query Frame = 2 
Query:  326 FCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDH-EIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSETDS---------YPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL 937
            FC+++VLL+ MQ+ LG S + LG YL    P L  ++C +WA +PL  +G  G  FC  +  + + RS+ S  ++     +S+++  L L ASI SG  G  I+     C    IR  ++T +         Y  +CC D  T  +  H          VC+     N  +  + +    +TCRY+ S++KDY ILQS+LM VA GVC+W  ++
Sbjct:  470 FCQLHVLLVIMQIVLGVSTTALGFYLHWKIPLLPLEECAFWAAIPLSFTGMIGSLFCFENRCQRFNRSY-SVSMRRATGILSLISCVLCLVASIFSGQKGNVIATSGHPC--QSIRLTTQTKNATQSSGLPEYEHQCCSD--TSGLDGHN---------VCQCLNMRNQVIWSYHK----ITCRYLISSVKDYLILQSALMAVAAGVCLWFWVV 665
[Close]
BLAST of SMED30021707 vs. TrEMBL
Match: A0A1S8X932 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_01139 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 3.684e-21
Identity = 69/226 (30.53%), Postives = 110/226 (48.67%), Query Frame = 2 
Query:  326 FCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLP----LLC----------SGAFGLYFCLSDH-EIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSET-------DSYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL 937
            FC+++VLL+ MQ+ LG S + LG YL    P L  ++C +WA +P    L+C          +G  G  FC  +  +++ RSH S  ++     +++++  L L ASI SG  G  I+     C +     H++          Y  +CC D  +            D   VC+     N  +  + +    +TCRY+ S++KDY ILQS+LM VA GVC+W  ++
Sbjct:  470 FCQLHVLLVIMQIVLGMSTTALGFYLHWKIPLLPLEECAFWAAIPGTNLLVCFSYLTFQLSFTGIIGSVFCFENRCQLFNRSH-SVSMRRATGILTLISCVLCLVASIFSGQKGNVIASSGHPCQVVRPTTHTKNVTQSLALPEYEHQCCSDTSSL-----------DGHNVCQCLNMRNQVIWSYHK----ITCRYLISSVKDYLILQSALMAVAAGVCLWFWVV 679
[Close]
BLAST of SMED30021707 vs. Planmine SMEST
Match: SMESG000063964.1 (SMESG000063964.1)

HSP 1 Score: 770.77 bits (1989), Expect = 0.000e+0
Identity = 370/371 (99.73%), Postives = 370/371 (99.73%), Query Frame = 2 
Query:   26 METENNQLICNFPLSKPRVNSAPEVLAVINKKHKSRGPVEKHLKHKRKSSQSSISIDVFRSPNNNFKNTESYLSISRNFSIVSGRRLSSMEQNKEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSETDSYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVILQIKDEQPNMIDQENISRYQNHRQTGLDESPSPSYLRFFPNQNGPNYINKIISPSPKELQIPKVIVHE 1138
            METENNQLICNFPL KPRVNSAPEVLAVINKKHKSRGPVEKHLKHKRKSSQSSISIDVFRSPNNNFKNTESYLSISRNFSIVSGRRLSSMEQNKEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSETDSYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVILQIKDEQPNMIDQENISRYQNHRQTGLDESPSPSYLRFFPNQNGPNYINKIISPSPKELQIPKVIVHE
Sbjct:    1 METENNQLICNFPLKKPRVNSAPEVLAVINKKHKSRGPVEKHLKHKRKSSQSSISIDVFRSPNNNFKNTESYLSISRNFSIVSGRRLSSMEQNKEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSETDSYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVILQIKDEQPNMIDQENISRYQNHRQTGLDESPSPSYLRFFPNQNGPNYINKIISPSPKELQIPKVIVHE 371
[Close]
BLAST of SMED30021707 vs. Planmine SMEST
Match: SMESG000071184.1 (SMESG000071184.1)

HSP 1 Score: 181.415 bits (459), Expect = 4.758e-53
Identity = 115/331 (34.74%), Postives = 179/331 (54.08%), Query Frame = 2 
Query:   68 SKPRVNSAPEVLAVINKKHK--SRGPVEKHLKHKRKSSQSSISI--------DVFRSPNNNFKNTESYLSISRNFSIVSG---RRLSSMEQNKEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTC--DLHFIREHSETDSYP-DKCCEDKHTHPMPRHKLLPMNDTMCVCK-TAPFGNFSL-LKFER-----EYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL----QIKDEQPNMIDQEN 979
            ++PR +SAP V+  + K+    S+   +K+ K +     ++I++        D+  +  +   +   Y S  R  S++     RR S ++Q+KEK E+  CRINVLL+ MQLGLG SM+ LG+YL ++SP+LT KDC YWA  PLL +G  GLYFC ++ +++K+  +S +V+   A  S+V + L   AS  SG HG W++ +   C  +LH   E     ++P   CC+   T  +P+         +C C       N S+ + + +      Y  V C YVFS+LKDY ILQ +L  VA GV IW+ +L    ++ D+  N+I   N
Sbjct:    4 NRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQLGLGSSMAALGLYLHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQ---TLQIPK--------LICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRN 323
[Close]
BLAST of SMED30021707 vs. Planmine SMEST
Match: SMESG000071184.1 (SMESG000071184.1)

HSP 1 Score: 181.03 bits (458), Expect = 5.345e-53
Identity = 115/331 (34.74%), Postives = 179/331 (54.08%), Query Frame = 2 
Query:   68 SKPRVNSAPEVLAVINKKHK--SRGPVEKHLKHKRKSSQSSISI--------DVFRSPNNNFKNTESYLSISRNFSIVSG---RRLSSMEQNKEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTC--DLHFIREHSETDSYP-DKCCEDKHTHPMPRHKLLPMNDTMCVCK-TAPFGNFSL-LKFER-----EYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL----QIKDEQPNMIDQEN 979
            ++PR +SAP V+  + K+    S+   +K+ K +     ++I++        D+  +  +   +   Y S  R  S++     RR S ++Q+KEK E+  CRINVLL+ MQLGLG SM+ LG+YL ++SP+LT KDC YWA  PLL +G  GLYFC ++ +++K+  +S +V+   A  S+V + L   AS  SG HG W++ +   C  +LH   E     ++P   CC+   T  +P+         +C C       N S+ + + +      Y  V C YVFS+LKDY ILQ +L  VA GV IW+ +L    ++ D+  N+I   N
Sbjct:    7 NRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQLGLGSSMAALGLYLHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQ---TLQIPK--------LICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRN 326
[Close]
BLAST of SMED30021707 vs. Planmine SMEST
Match: SMESG000033408.1 (SMESG000033408.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.504e-42
Identity = 86/241 (35.68%), Postives = 130/241 (53.94%), Query Frame = 2 
Query:  305 KEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSETDSYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL-------------QIKDEQPNMIDQENISR 988
            ++  + + C+I VLL+ M++ LG +M+GLG++L   S S+  KDC +WAG PLL SG F ++     +++ KR  +  L+ I C  +S + + L L +SI SG HG WIS  S  C     ++ +  +     CC D HT  +       +N T C C+ A    +S       Y  + CRYVFSALKDY ILQ +LMGV +GVC+W++ +             Q +  QPN  DQ ++ +
Sbjct:  169 RDSLQHRLCKIRVLLVCMKILLGSTMTGLGIFLLMASKSIKVKDCAFWAGGPLLFSGLFEVFIYNWRNKLRKRMKICYLMTIACGVISFICSILCLLSSIFSGVHGNWISGHSDNC---LSKDDNNQNDMLLACCYDIHTKGINS----SINST-CFCREARENIYSKPIITMTYENLNCRYVFSALKDYLILQCALMGVGSGVCLWLLQMTIQRLIQDCSTTSQTRSGQPNTDDQNSVGQ 401
[Close]
BLAST of SMED30021707 vs. Planmine SMEST
Match: SMESG000033408.1 (SMESG000033408.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.546e-42
Identity = 86/241 (35.68%), Postives = 130/241 (53.94%), Query Frame = 2 
Query:  305 KEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTCDLHFIREHSETDSYPDKCCEDKHTHPMPRHKLLPMNDTMCVCKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL-------------QIKDEQPNMIDQENISR 988
            ++  + + C+I VLL+ M++ LG +M+GLG++L   S S+  KDC +WAG PLL SG F ++     +++ KR  +  L+ I C  +S + + L L +SI SG HG WIS  S  C     ++ +  +     CC D HT  +       +N T C C+ A    +S       Y  + CRYVFSALKDY ILQ +LMGV +GVC+W++ +             Q +  QPN  DQ ++ +
Sbjct:  170 RDSLQHRLCKIRVLLVCMKILLGSTMTGLGIFLLMASKSIKVKDCAFWAGGPLLFSGLFEVFIYNWRNKLRKRMKICYLMTIACGVISFICSILCLLSSIFSGVHGNWISGHSDNC---LSKDDNNQNDMLLACCYDIHTKGINS----SINST-CFCREARENIYSKPIITMTYENLNCRYVFSALKDYLILQCALMGVGSGVCLWLLQMTIQRLIQDCSTTSQTRSGQPNTDDQNSVGQ 402
The following BLAST results are available for this feature:
BLAST of SMED30021707 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RIR97.238e-3133.05Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A504YEC95.938e-2931.80Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A419Q0G88.623e-2429.46Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A4S2MH705.519e-2233.18Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A1S8X9323.684e-2130.53Uncharacterized protein OS=Opisthorchis viverrini ... [more]
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BLAST of SMED30021707 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021707 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000063964.10.000e+099.73SMESG000063964.1[more]
SMESG000071184.14.758e-5334.74SMESG000071184.1[more]
SMESG000071184.15.345e-5334.74SMESG000071184.1[more]
SMESG000033408.11.504e-4235.68SMESG000033408.1[more]
SMESG000033408.11.546e-4235.68SMESG000033408.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021707 ID=SMED30021707|Name=SMED30021707|organism=Schmidtea mediterranea sexual|type=transcript|length=1227bp
TAAATAGATAGATTTGCATTTTCTGATGGAAACAGAGAACAATCAACTGA
TTTGCAATTTTCCCTTGTCGAAGCCCCGTGTCAATTCAGCCCCCGAAGTG
CTCGCAGTCATAAACAAGAAACACAAATCCAGAGGTCCGGTTGAGAAGCA
TTTGAAGCACAAACGAAAATCATCTCAAAGTTCGATTTCGATTGACGTTT
TTCGGTCCCCGAATAATAATTTCAAAAACACTGAAAGTTACTTGTCAATT
TCTAGAAATTTTTCAATCGTTTCTGGCCGGCGCCTTTCTAGCATGGAACA
GAACAAGGAGAAATTCGAAATCCAATTTTGTCGGATAAATGTTTTATTAA
TAAGCATGCAGCTGGGGTTGGGATTTTCAATGTCAGGGCTTGGGGTTTAT
TTGAGATCAGTTTCCCCATCACTCACTTGGAAAGACTGCACATATTGGGC
TGGATTACCGCTACTCTGCAGTGGAGCATTCGGTCTTTATTTTTGTTTGT
CTGACCACGAAATTTACAAACGATCACATTTATCTAGACTTGTCAAGATT
GTCTGTGCTTGCATGTCTGTAGTGACAGCTGCCCTAACTTTATCAGCAAG
CATCATGTCTGGTACTCATGGAAAATGGATATCTGATTTTTCATCAACGT
GTGACCTCCATTTTATTAGGGAACACAGCGAAACGGACAGCTATCCGGAT
AAATGCTGTGAGGATAAACATACCCATCCAATGCCAAGGCACAAGTTGCT
ACCTATGAATGACACGATGTGTGTTTGCAAAACTGCTCCTTTTGGTAATT
TTTCGCTACTAAAGTTCGAACGAGAATATGCTGCAGTGACTTGCCGTTAT
GTGTTTTCAGCTCTCAAAGATTATTTCATTCTCCAAAGCTCATTAATGGG
AGTTGCCACTGGAGTTTGTATTTGGATAGTTATACTTCAAATAAAGGATG
AACAGCCTAATATGATAGATCAAGAAAATATTTCTCGTTATCAAAATCAC
AGACAAACCGGATTAGATGAATCTCCATCTCCAAGCTATTTAAGATTTTT
CCCAAACCAAAACGGCCCAAATTATATCAACAAAATTATTTCTCCTTCGC
CTAAAGAATTACAAATCCCGAAAGTTATTGTCCACGAAATTATAGATAAA
AATTAATTTTGATTTATTATTATAATAAAATTTTGCAATTTAAATTATTA
TTATTTGAATAAATAATTATTGCTTAA
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protein sequence of SMED30021707-orf-1

>SMED30021707-orf-1 ID=SMED30021707-orf-1|Name=SMED30021707-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=377bp
METENNQLICNFPLSKPRVNSAPEVLAVINKKHKSRGPVEKHLKHKRKSS
QSSISIDVFRSPNNNFKNTESYLSISRNFSIVSGRRLSSMEQNKEKFEIQ
FCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSG
AFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGK
WISDFSSTCDLHFIREHSETDSYPDKCCEDKHTHPMPRHKLLPMNDTMCV
CKTAPFGNFSLLKFEREYAAVTCRYVFSALKDYFILQSSLMGVATGVCIW
IVILQIKDEQPNMIDQENISRYQNHRQTGLDESPSPSYLRFFPNQNGPNY
INKIISPSPKELQIPKVIVHEIIDKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR030429Sarcospan
Vocabulary: cellular component
TermDefinition
GO:0005886plasma membrane
GO:0005887integral component of plasma membrane
GO:0016010dystrophin-associated glycoprotein complex
GO:0016020membrane
GO:0016021integral component of membrane
GO:0030054cell junction
GO:0030133transport vesicle
GO:0042383sarcolemma
GO:0045202synapse
GO:0045211postsynaptic membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR030429SarcospanPANTHERPTHR15260FAMILY NOT NAMEDcoord: 77..307
NoneNo IPR availableTMHMMTMhelixcoord: 141..159
NoneNo IPR availableTMHMMTMhelixcoord: 172..194
NoneNo IPR availableTMHMMTMhelixcoord: 104..126
NoneNo IPR availableTMHMMTMhelixcoord: 284..306