SMED30021704

Overview
NameSMED30021704
Smed IDSMED30021704
Length (bp)359
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30021704 (SMED30021704) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021704 (SMED30021704) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021704 (SMED30021704) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021704 (SMED30021704) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021704

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30021704 dd_Smed_v4_33112_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021704 dd_Smed_v4_33112_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30021704 dd_Smed_v4_33112_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30021704 vs. Ensembl Zebrafish
Match: CABZ01030006.1 (pep scaffold:GRCz11:KN150258.1:33006:36502:-1 gene:ENSDARG00000114113.1 transcript:ENSDART00000182402.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01030006.1)

HSP 1 Score: 44.2838 bits (103), Expect = 8.161e-6
Identity = 17/39 (43.59%), Postives = 26/39 (66.67%), Query Frame = 2 
Query:   26 EKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTI 142
            E  +K W   LP++ F LN+ IQ++ G++PAE+  GR I
Sbjct:  154 EDNHKTWDTYLPELRFALNSAIQESIGMTPAELHLGRKI 192
[Close]
BLAST of SMED30021704 vs. UniProt/SwissProt
Match: sp|Q7LHG5|YI31B_YEAST (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 53.9138 bits (128), Expect = 2.594e-8
Identity = 37/134 (27.61%), Postives = 62/134 (46.27%), Query Frame = 2 
Query:   38 KEWSEILPDVEFMLNATIQQTTGLSPAEIVFG------------RTICREWTETEIDKRRKEIISKQNEEVNKIPI---TSKN-------CNIGDHILVKRE------NIKQMDECYQGPYEIIQKVYERSYKL 355
            + W   LP +EF+ N+T  +T G SP EI  G                R +T  E+ K  K +  +  E++    I   T+ N        NIGDH+LV R+         ++ + Y GP+ +++K+ + +Y+L
Sbjct: 1317 QNWHVYLPQIEFVYNSTPTRTLGKSPFEIDLGYLPNTPAIKSDDEVNARSFTAVELAKHLKALTIQTKEQLEHAQIEMETNNNQRRKPLLLNIGDHVLVHRDAYFKKGAYMKVQQIYVGPFRVVKKINDNAYEL 1450
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BLAST of SMED30021704 vs. UniProt/SwissProt
Match: sp|Q99315|YG31B_YEAST (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 53.9138 bits (128), Expect = 2.946e-8
Identity = 37/134 (27.61%), Postives = 62/134 (46.27%), Query Frame = 2 
Query:   38 KEWSEILPDVEFMLNATIQQTTGLSPAEIVFG------------RTICREWTETEIDKRRKEIISKQNEEVNKIPI---TSKN-------CNIGDHILVKRE------NIKQMDECYQGPYEIIQKVYERSYKL 355
            + W   LP +EF+ N+T  +T G SP EI  G                R +T  E+ K  K +  +  E++    I   T+ N        NIGDH+LV R+         ++ + Y GP+ +++K+ + +Y+L
Sbjct: 1291 QNWHVYLPQIEFVYNSTPTRTLGKSPFEIDLGYLPNTPAIKSDDEVNARSFTAVELAKHLKALTIQTKEQLEHAQIEMETNNNQRRKPLLLNIGDHVLVHRDAYFKKGAYMKVQQIYVGPFRVVKKINDNAYEL 1424
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BLAST of SMED30021704 vs. TrEMBL
Match: A0A0C2J0B9 (Uncharacterized protein OS=Thelohanellus kitauei OX=669202 GN=RF11_04774 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.111e-9
Identity = 39/132 (29.55%), Postives = 70/132 (53.03%), Query Frame = 2 
Query:    8 LAVTLNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFG------RTICREWTETEIDKRRKEIISKQNEE--------VNKIP-ITSKNC----NIGDHILVKRENIKQMDECYQGPYEIIQKVYERS 346
            L + ++E     W E L  V+F + A+I  +T  SPA++V+G        +CR  +E+ +D+    I  K+ ++        VN++  IT KNC     IGD +LVK  +  ++D  ++GP+E + ++  R+
Sbjct:   17 LTIAVSEVDIDRWDENLEQVQFAIRASISSSTKFSPAKLVYGCELRLPIDLCRPISESSLDQGNYHIFVKKFKKELEDTFNIVNQLSRITIKNCVTNFKIGDMVLVKELSRTKLDPLFEGPFEELSRLVRRT 148
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BLAST of SMED30021704 vs. TrEMBL
Match: A0A5N5QCT6 (Retrotransposable element Tf2 OS=Ceratobasidium theobromae OX=1582974 GN=CTheo_7144 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.666e-7
Identity = 39/132 (29.55%), Postives = 67/132 (50.76%), Query Frame = 2 
Query:   17 TLNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTE----TEI---DKRRKEIISKQNEEVN------KIPITSKNCNIGDHILVKRENIK------QMDECYQGPYEIIQKVYERSYKL 355
            T    R  +W+  LP  EF  N  + ++T  +P EIV+GRT+  EW +    TEI   D+    I + Q+E         + P +  + N+GD +++ R NI+      ++D    GP+ I++K+   ++KL
Sbjct:  841 TFGNTRQDDWAHWLPLAEFAYNNHVNRSTKKAPFEIVYGRTL--EWKDSGRTTEIIHADEWGTRIRAVQDEARACLEMGWRDPGSVSDLNVGDRVMLLRTNIRSDRPSQKLDSKKLGPFTILRKISSHAFKL 970
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BLAST of SMED30021704 vs. TrEMBL
Match: A0A5N5Q8Y6 (Retrotransposable element Tf2 OS=Ceratobasidium theobromae OX=1582974 GN=CTheo_8672 PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.012e-7
Identity = 39/131 (29.77%), Postives = 64/131 (48.85%), Query Frame = 2 
Query:   32 RYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTI-------------CREW----TETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENI------KQMDECYQGPYEIIQKVYERSYKL 355
            R  +W+E LP  EF+ N ++ + TG +P EIVFG+T+               EW    +E + + +     + Q EEV      S+    G+ +L+  ENI      K++D+   GP+EI  +V   +Y+L
Sbjct: 1301 RQSDWAEWLPMAEFVYNNSVNRMTGKTPFEIVFGKTMEWDVADKRTMNIRAEEWGKMISEAQAEAKAALKFNHQEEEV------SREFVPGEKVLLLAENIKGERESKKLDDKRMGPFEIKSRVSSHAYEL 1425
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BLAST of SMED30021704 vs. TrEMBL
Match: A0A0C2MGF5 (Retrovirus-related Pol polyprotein OS=Thelohanellus kitauei OX=669202 GN=RF11_15562 PE=4 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.011e-6
Identity = 35/129 (27.13%), Postives = 61/129 (47.29%), Query Frame = 2 
Query:   20 LNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTI----------------CREWTETEIDKRRKEIISK----QNEEVNKIPITSKN-----CNIGDHILVKRENIKQMDECYQGPYEIIQK 331
            + +  Y  W E LP++ F +  TI ++TG +P  +V+GR +                  +  E+ +DKR K++       +  + N   I  K       +IGD I+V++     +++ Y GPYEII+K
Sbjct:  110 IADNNYTRWDEKLPEILFSMRNTISKSTGFAPTSLVYGRKLRSRTEAIYDINKINSYNTQINESSMDKRIKQLTEARANIEKSQTNYKKIYDKGHIPCEFDIGDKIMVRKYKTNSLEQPYVGPYEIIEK 238
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BLAST of SMED30021704 vs. TrEMBL
Match: Q875I4 (TyB3p (Fragment) OS=Saccharomyces paradoxus OX=27291 PE=4 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.162e-6
Identity = 38/134 (28.36%), Postives = 63/134 (47.01%), Query Frame = 2 
Query:   38 KEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTI------------CREWTETEIDKRRKEII--SKQNEEVNKIPITSKN--------CNIGDHILVKRE------NIKQMDECYQGPYEIIQKVYERSYKL 355
            + W   LP +EF+ N+T  +T G SP EI  G T              R +T  E+ +  K I   +K+  E  +I + + N         NIGDH+LV R+         ++   Y GP+ +++K+ + +Y+L
Sbjct: 1014 QNWHTYLPQIEFVYNSTPTRTLGKSPFEIDLGYTPNAPTIKTECEINARSFTAVELARHLKAITIQTKERLESAQIEMETNNNQRRKTLLLNIGDHVLVHRDAYFKKGTYMKVQPIYVGPFRVVKKINDNAYEL 1147
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BLAST of SMED30021704 vs. Ensembl Cavefish
Match: ENSAMXT00000030217.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:12351169:12363564:1 gene:ENSAMXG00000035335.1 transcript:ENSAMXT00000030217.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.8246 bits (107), Expect = 2.387e-6
Identity = 20/45 (44.44%), Postives = 29/45 (64.44%), Query Frame = 2 
Query:    2 YNLAVTLNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGR 136
            ++L  TL  +R + WS  LP + F  N T+ Q+TG SP E++FGR
Sbjct: 1063 HDLLRTLPAERKRRWSHHLPQLLFAYNTTVHQSTGHSPYELMFGR 1107
[Close]
BLAST of SMED30021704 vs. Ensembl Cavefish
Match: ENSAMXT00000054717.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000181.1:337738:340615:1 gene:ENSAMXG00000038319.1 transcript:ENSAMXT00000054717.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 44.2838 bits (103), Expect = 7.097e-6
Identity = 32/139 (23.02%), Postives = 62/139 (44.60%), Query Frame = 2 
Query:   32 RYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICR-----------------------EWTETEIDKRRKEIISKQNEEVNKIPITSKNCNI----GDHILVKR----ENIKQMDECYQGPYEIIQKVYERSYKL 355
            ++  W + LP + F L  +  ++TG SPA++++ R +                         E T  +ID   + +  K + E +K     K C++    GD + +K     +   ++   Y GPY+++QK+ E +Y+L
Sbjct:  245 KHNAWDKYLPQICFALRTSPHKSTGFSPAKMLYRRELTTHLYLLTKPSTDGLVDHLSQPETPEETVCKIDDHAR-VSLKSSHERHKRHYNKKRCSVTYEPGDLVHLKNRPRSDAAAKLAPVYSGPYKVVQKLSEVNYRL 382
[Close]
BLAST of SMED30021704 vs. Planmine SMEST
Match: SMESG000052308.1 (SMESG000052308.1)

HSP 1 Score: 239.195 bits (609), Expect = 2.717e-79
Identity = 115/116 (99.14%), Postives = 116/116 (100.00%), Query Frame = 2 
Query:   11 AVTLNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR 358
            AVTLNEKRY+EWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR
Sbjct:  242 AVTLNEKRYEEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR 357
[Close]
BLAST of SMED30021704 vs. Planmine SMEST
Match: SMESG000052308.1 (SMESG000052308.1)

HSP 1 Score: 239.195 bits (609), Expect = 3.343e-79
Identity = 115/116 (99.14%), Postives = 116/116 (100.00%), Query Frame = 2 
Query:   11 AVTLNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR 358
            AVTLNEKRY+EWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR
Sbjct:  246 AVTLNEKRYEEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR 361
[Close]
BLAST of SMED30021704 vs. Planmine SMEST
Match: SMESG000022429.1 (SMESG000022429.1)

HSP 1 Score: 219.935 bits (559), Expect = 4.517e-72
Identity = 105/107 (98.13%), Postives = 106/107 (99.07%), Query Frame = 2 
Query:   38 KEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR 358
            KEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILV RENIKQMD+CYQGPYEIIQKVYERSYKLR
Sbjct:  245 KEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICREWTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVNRENIKQMDKCYQGPYEIIQKVYERSYKLR 351
[Close]
BLAST of SMED30021704 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 88.1965 bits (217), Expect = 2.245e-21
Identity = 48/119 (40.34%), Postives = 80/119 (67.23%), Query Frame = 2 
Query:    8 LAVTLNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICR-EW-TETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKLR 358
            +  +LNE   K W++I+P++++ LNAT+Q+TTG+SPAEI+FGR I R +W +  EI++   E   +      KI  T +N  + D +L+K+E   + D  ++GPY++I+K++ERSY L+
Sbjct: 3405 INASLNEGGRKNWADIVPEIKYTLNATVQKTTGVSPAEIIFGRKIDRMKWYSNKEINREDMEKRIEDKTLKPKISKTVRNFEMEDVVLIKQEIRNKDDARWEGPYKVIKKIHERSYLLK 3523
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BLAST of SMED30021704 vs. Planmine SMEST
Match: SMESG000005477.1 (SMESG000005477.1)

HSP 1 Score: 75.0998 bits (183), Expect = 7.944e-17
Identity = 41/107 (38.32%), Postives = 72/107 (67.29%), Query Frame = 2 
Query:   44 WSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICR-EW-TETEIDKRR-KEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDECYQGPYEIIQKVYERSYKL 355
            W+E++PD+E++LNAT+Q+ TG+S AEI+ GR I R +W    E+++ R  + +  + +   KI  T+++  +GD +L+K+E   +  E Y+GPY I+++ +ER Y L
Sbjct:  629 WAELIPDIEYVLNATVQKPTGVSSAEIILGRIIDRWQWNINNEVNRERIIQRLEAKRQNAKKIN-TTRSLEVGDKVLLKQEVKNKDQERYEGPYTILKRNHERRYLL 734
The following BLAST results are available for this feature:
BLAST of SMED30021704 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CABZ01030006.18.161e-643.59pep scaffold:GRCz11:KN150258.1:33006:36502:-1 gene... [more]
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BLAST of SMED30021704 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q7LHG5|YI31B_YEAST2.594e-827.61Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomy... [more]
sp|Q99315|YG31B_YEAST2.946e-827.61Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomy... [more]
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BLAST of SMED30021704 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0C2J0B92.111e-929.55Uncharacterized protein OS=Thelohanellus kitauei O... [more]
A0A5N5QCT63.666e-729.55Retrotransposable element Tf2 OS=Ceratobasidium th... [more]
A0A5N5Q8Y69.012e-729.77Retrotransposable element Tf2 OS=Ceratobasidium th... [more]
A0A0C2MGF51.011e-627.13Retrovirus-related Pol polyprotein OS=Thelohanellu... [more]
Q875I41.162e-628.36TyB3p (Fragment) OS=Saccharomyces paradoxus OX=272... [more]
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BLAST of SMED30021704 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000030217.12.387e-644.44pep primary_assembly:Astyanax_mexicanus-2.0:16:123... [more]
ENSAMXT00000054717.17.097e-623.02pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of SMED30021704 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021704 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052308.12.717e-7999.14SMESG000052308.1[more]
SMESG000052308.13.343e-7999.14SMESG000052308.1[more]
SMESG000022429.14.517e-7298.13SMESG000022429.1[more]
SMESG000029259.12.245e-2140.34SMESG000029259.1[more]
SMESG000005477.17.944e-1738.32SMESG000005477.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021704 ID=SMED30021704|Name=SMED30021704|organism=Schmidtea mediterranea sexual|type=transcript|length=359bp
ATACAATTTAGCCGTTACATTGAATGAAAAACGATATAAAGAGTGGTCAG
AAATATTACCAGATGTCGAATTCATGTTAAATGCAACAATACAGCAGACA
ACGGGATTATCACCTGCAGAAATAGTATTTGGTCGGACGATATGCCGCGA
ATGGACTGAAACAGAAATAGACAAACGGAGAAAAGAAATTATAAGTAAAC
AAAATGAAGAAGTAAATAAAATACCAATAACTTCTAAAAATTGCAATATT
GGAGATCATATTTTAGTAAAGCGAGAAAATATAAAACAAATGGATGAATG
CTATCAGGGACCGTATGAAATTATACAAAAGGTTTATGAAAGAAGCTATA
AACTGAGAG
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protein sequence of SMED30021704-orf-1

>SMED30021704-orf-1 ID=SMED30021704-orf-1|Name=SMED30021704-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=119bp
YNLAVTLNEKRYKEWSEILPDVEFMLNATIQQTTGLSPAEIVFGRTICRE
WTETEIDKRRKEIISKQNEEVNKIPITSKNCNIGDHILVKRENIKQMDEC
YQGPYEIIQKVYERSYKLR
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0003676nucleic acid binding
GO:0003677DNA binding
GO:0003723RNA binding
GO:0003824catalytic activity
GO:0003887DNA-directed DNA polymerase activity
GO:0003964RNA-directed DNA polymerase activity
GO:0004190aspartic-type endopeptidase activity
GO:0004518nuclease activity
GO:0004519endonuclease activity
GO:0004523RNA-DNA hybrid ribonuclease activity
GO:0004540ribonuclease activity
GO:0005524ATP binding
GO:0008233peptidase activity
GO:0008270zinc ion binding
GO:0016740transferase activity
GO:0016779nucleotidyltransferase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0000943retrotransposon nucleocapsid
GO:0005634nucleus
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0006278RNA-dependent DNA biosynthetic process
GO:0006310DNA recombination
GO:0006508proteolysis
GO:0008152metabolic process
GO:0015074DNA integration
GO:0032197transposition, RNA-mediated
GO:0071897DNA biosynthetic process
GO:0090305nucleic acid phosphodiester bond hydrolysis
GO:0090501RNA phosphodiester bond hydrolysis
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolytic