Zinc finger MYM-type protein 1

Overview
NameZinc finger MYM-type protein 1
Smed IDSMED30021676
Length (bp)1041
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Zinc finger MYM-type protein 1 (SMED30021676) t-SNE clustered cells

Violin plots show distribution of expression levels for Zinc finger MYM-type protein 1 (SMED30021676) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Zinc finger MYM-type protein 1 (SMED30021676) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Zinc finger MYM-type protein 1 (SMED30021676) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021676

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30021676 SmedASXL_071165SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000043220.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:36411169:36451375:1 gene:ENSXETG00000018966.1 transcript:ENSXETT00000043220.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 3.011e-7
Identity = 24/44 (54.55%), Postives = 34/44 (77.27%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRAS 263
            PNI+ A + FLT P+TVA+  RSFSK K+IKN+L S++ ++R S
Sbjct:  645 PNIDTAFRIFLTSPVTVASCKRSFSKLKLIKNYLRSSMEQERLS 688          
BLAST of Zinc finger MYM-type protein 1 vs. TrEMBL
Match: A0A2H8TX80 (Zinc finger MYM-type protein 1 OS=Melanaphis sacchari OX=742174 GN=ZMYM1_24 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.136e-7
Identity = 30/42 (71.43%), Postives = 37/42 (88.10%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQR 257
            PNIEIAL+ FLT P+TVA+ +RSFSK KIIKN+L STLG++R
Sbjct:  754 PNIEIALRIFLTLPVTVASCERSFSKLKIIKNYLRSTLGQER 795          
BLAST of Zinc finger MYM-type protein 1 vs. TrEMBL
Match: J9LV19 (Dimer_Tnp_hAT domain-containing protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 1.603e-7
Identity = 26/44 (59.09%), Postives = 35/44 (79.55%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRAS 263
            PN+EI  + FLT P+T AT +RSFSK K+IKN+L ST+G++R S
Sbjct:   57 PNVEIVFRIFLTMPVTAATCERSFSKLKLIKNYLRSTMGQERLS 100          
BLAST of Zinc finger MYM-type protein 1 vs. TrEMBL
Match: A0A2S2PFL8 (Zinc finger MYM-type protein 1 (Fragment) OS=Schizaphis graminum OX=13262 GN=ZMYM1_81 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.438e-7
Identity = 27/44 (61.36%), Postives = 35/44 (79.55%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRAS 263
            PNIEI  + FLT P+T AT +RSFSK K+IKN+L ST+G++R S
Sbjct:  145 PNIEIVFRIFLTMPVTAATCERSFSKLKLIKNYLRSTMGQERLS 188          
BLAST of Zinc finger MYM-type protein 1 vs. TrEMBL
Match: A0A026WET7 (Zinc finger MYM-type protein (Fragment) OS=Ooceraea biroi OX=2015173 GN=X777_05687 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.830e-7
Identity = 25/45 (55.56%), Postives = 38/45 (84.44%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRASA 266
            PNI +AL+ FLT P+++A+ +RSFSK K+IKN+L ST+G++R S+
Sbjct:   11 PNINVALRIFLTLPVSIASCERSFSKLKLIKNYLRSTMGQERLSS 55          
BLAST of Zinc finger MYM-type protein 1 vs. TrEMBL
Match: J9LQW5 (Dimer_Tnp_hAT domain-containing protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 6.926e-7
Identity = 31/70 (44.29%), Postives = 47/70 (67.14%), Query Frame = 3
Query:   63 GTVANEAGFS--SFKKAPLNDQVFNPNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRASA 266
            G+V++E      S  K    D+   PNIEIA++ FL+KP+T A+ +RSFSK K+IK +L ST+ ++R S+
Sbjct:   71 GSVSDEKSVELVSNLKEYQEDKESYPNIEIAIRIFLSKPVTTASRERSFSKLKLIKTYLRSTMAQERLSS 140          
BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Cavefish
Match: ENSAMXT00000036272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:11935414:11938921:1 gene:ENSAMXG00000041670.1 transcript:ENSAMXT00000036272.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 6.918e-8
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 3
Query:  120 QVFNPNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRASA 266
            Q   PN+ IAL+  LT P+TVA+ +RSFSK K+IK +L ST+G++R S 
Sbjct:  740 QEVYPNLWIALRIALTLPVTVASAERSFSKLKLIKTYLRSTMGQERLSG 788          
BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Cavefish
Match: ENSAMXT00000042930.1 (pep primary_assembly:Astyanax_mexicanus-2.0:12:25471427:25473738:-1 gene:ENSAMXG00000037442.1 transcript:ENSAMXT00000042930.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 9.621e-7
Identity = 24/44 (54.55%), Postives = 33/44 (75.00%), Query Frame = 3
Query:  135 NIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRASA 266
            ++  ALK FLT P+TVA+ +RSFSK K+IKN+L ST+ + R S 
Sbjct:  560 DVATALKLFLTIPVTVASAERSFSKLKMIKNYLRSTMSQDRLSG 603          
BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Cavefish
Match: ENSAMXT00000035789.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000101.1:2843018:2852765:1 gene:ENSAMXG00000037827.1 transcript:ENSAMXT00000035789.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 1.182e-6
Identity = 24/44 (54.55%), Postives = 33/44 (75.00%), Query Frame = 3
Query:  135 NIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRASA 266
            ++  ALK FLT P+TVA+ +RSFSK K+IKN+L ST+ + R S 
Sbjct:  544 DVATALKLFLTIPVTVASAERSFSKLKMIKNYLRSTMSQDRLSG 587          
BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Cavefish
Match: ENSAMXT00000055782.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001476.1:1798:4262:-1 gene:ENSAMXG00000042376.1 transcript:ENSAMXT00000055782.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 2.141e-6
Identity = 23/41 (56.10%), Postives = 32/41 (78.05%), Query Frame = 3
Query:  135 NIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQR 257
            N+ IAL+  LT P+TVA+ +RSFS  K+IKNFL ST+ ++R
Sbjct:  668 NLSIALRLLLTLPITVASGERSFSSLKLIKNFLRSTMSQER 708          
BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Cavefish
Match: ENSAMXT00000033195.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:11277158:11279506:-1 gene:ENSAMXG00000030798.1 transcript:ENSAMXT00000033195.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.6766 bits (117), Expect = 3.815e-6
Identity = 22/41 (53.66%), Postives = 31/41 (75.61%), Query Frame = 3
Query:  135 NIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQR 257
             I IAL+ F T P+TVA  +R+FSK K++KN+L ST+G+ R
Sbjct:  703 EICIALRIFCTLPITVAGGERAFSKLKLVKNYLRSTMGQDR 743          
BLAST of Zinc finger MYM-type protein 1 vs. Planmine SMEST
Match: SMESG000078916.1 (SMESG000078916.1)

HSP 1 Score: 52.7582 bits (125), Expect = 3.024e-8
Identity = 24/46 (52.17%), Postives = 34/46 (73.91%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRASAP 269
            PN+  ALK  LT P++VA+ +RSFS+ K+IKN+L S+L + R S P
Sbjct:   74 PNLYFALKILLTMPVSVASGERSFSRLKLIKNYLRSSLTQNRLSGP 119          
BLAST of Zinc finger MYM-type protein 1 vs. Planmine SMEST
Match: SMESG000004944.1 (SMESG000004944.1)

HSP 1 Score: 48.1358 bits (113), Expect = 4.034e-7
Identity = 22/52 (42.31%), Postives = 37/52 (71.15%), Query Frame = 3
Query:  108 PLNDQVFNPNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRAS 263
            P  D VF PN++IAL+  LT  +++A+ +RSFS+ K+I  +L +++G+ R S
Sbjct:   23 PYGDDVF-PNLQIALQVLLTISVSIASGERSFSRLKLILYYLRASMGQDRLS 73          
BLAST of Zinc finger MYM-type protein 1 vs. Planmine SMEST
Match: SMESG000026726.1 (SMESG000026726.1)

HSP 1 Score: 49.6766 bits (117), Expect = 5.810e-7
Identity = 22/45 (48.89%), Postives = 34/45 (75.56%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRASA 266
            PN+ IALK  LT P++VA+ +RSFS+ K+IK++L S++ + R S 
Sbjct:  113 PNLYIALKILLTMPVSVASGERSFSRLKLIKDYLRSSMTQNRLSG 157          
BLAST of Zinc finger MYM-type protein 1 vs. Planmine SMEST
Match: SMESG000077250.1 (SMESG000077250.1)

HSP 1 Score: 48.1358 bits (113), Expect = 8.880e-7
Identity = 23/44 (52.27%), Postives = 32/44 (72.73%), Query Frame = 3
Query:  132 PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRAS 263
            PN+ IALK  LT P+ VA+ +RSFS  K+IKN+L S++ + R S
Sbjct:   63 PNLYIALKILLTMPVNVASGERSFSLLKLIKNYLRSSMTQNRLS 106          
BLAST of Zinc finger MYM-type protein 1 vs. Planmine SMEST
Match: SMESG000048892.1 (SMESG000048892.1)

HSP 1 Score: 47.3654 bits (111), Expect = 1.518e-6
Identity = 30/76 (39.47%), Postives = 46/76 (60.53%), Query Frame = 3
Query:   63 GTVANEAGFSSFKKAPLND--QVFN-------PNIEIALKTFLTKPMTVATTDRSFSKPKIIKNFLMSTLGKQRAS 263
            G + N A + S +   LN+   +F        PN+ I+++ FLT P+TVA+  +SFSK KIIKN+  ST+ ++R S
Sbjct:   30 GEIDNVASYISSEMNALNNINYLFTNNLTYLFPNLVISIRIFLTLPVTVASGKKSFSKLKIIKNYPRSTIRQERLS 105          
The following BLAST results are available for this feature:
BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000043220.13.011e-754.55pep primary_assembly:Xenopus_tropicalis_v9.1:7:364... [more]
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2H8TX801.136e-771.43Zinc finger MYM-type protein 1 OS=Melanaphis sacch... [more]
J9LV191.603e-759.09Dimer_Tnp_hAT domain-containing protein OS=Acyrtho... [more]
A0A2S2PFL84.438e-761.36Zinc finger MYM-type protein 1 (Fragment) OS=Schiz... [more]
A0A026WET74.830e-755.56Zinc finger MYM-type protein (Fragment) OS=Oocerae... [more]
J9LQW56.926e-744.29Dimer_Tnp_hAT domain-containing protein OS=Acyrtho... [more]
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000036272.16.918e-853.06pep primary_assembly:Astyanax_mexicanus-2.0:20:119... [more]
ENSAMXT00000042930.19.621e-754.55pep primary_assembly:Astyanax_mexicanus-2.0:12:254... [more]
ENSAMXT00000035789.11.182e-654.55pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000055782.12.141e-656.10pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000033195.13.815e-653.66pep primary_assembly:Astyanax_mexicanus-2.0:16:112... [more]
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger MYM-type protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078916.13.024e-852.17SMESG000078916.1[more]
SMESG000004944.14.034e-742.31SMESG000004944.1[more]
SMESG000026726.15.810e-748.89SMESG000026726.1[more]
SMESG000077250.18.880e-752.27SMESG000077250.1[more]
SMESG000048892.11.518e-639.47SMESG000048892.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021676 ID=SMED30021676|Name=Zinc finger MYM-type protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1041bp
ATATTGGTATTAATATTAAAATGAATCCAATTAAGTTACTCACCTAAGAA
ATCTTAAGGATTGGGACAGTTGCAAATGAAGCCGGATTCTCCAGTTTCAA
AAAAGCGCCGCTAAATGACCAAGTATTTAACCCGAATATTGAAATAGCTC
TGAAAACATTTCTCACAAAGCCAATGACAGTAGCAACCACCGATAGGAGT
TTCAGCAAACCAAAAATTATTAAAAACTTCTTGATGTCAACGTTAGGCAA
ACAACGCGCATCAGCTCCAGTTCTTCTTTCCAGTTAAAAAACTTCGGTGA
ACTGATCTCTAAGTTTGTAAAGGTTAAAGCAAGAAAAGTTAAGCTTTAAA
CGCTTGATATAAGTCTAGAAAGTTACTTGAATTACTTTAAATTGCATGTT
TCTATCTTTATGCATGAGTGACACACTGACAATATTGTTTATTATACATC
AAGTGTTACTGAATTAACTCAACAATATGCTTCAATTAATTTAGGTTGTT
TAGTATTTCCTTATCTTCAATTTTTGATATTCAAAGACGATTATCAGACC
ATGTAAATATTGTCATAAATAAGAAAAACTCAAATATTTTTTTTGTAACG
ATTGGAAAAATTGCAAAGCTGCAATTGTTAAGGGTACCAAAAATAAATTA
GCCAGAGTCGCTGAAAAACCAGTTATCTTTTCATTGCTGGTAATTTTTGT
TTTCTTTTATACATGATTTGAATTTTTTTTCTCTATTTCTGGATACAGTT
TAAAATAGGAAGTTTTCTAGGTAATAATTTGCAATAGCGTAAATAAAAAC
TTCTTCAACCGCTCTGGCTTGTAAATTCTTCTCGTCATTTTCAGTTTCAC
ACGCTGCTTCAATAATATCATCAATTGCTTCAAACTTGCTTTTTATTTCT
AATGTTTCGCGTTTGTTCTGTTCATCTAATTCTTAAATTTGACCATCCGT
ATTGAAATCGTTGTAATTTTTACAAGTTATGTTCTCGTCATGTTTATAAA
TTATCACTTATTCATATTTTAATGCGTTGTTGAACAGCTTT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046983protein dimerization activity