SMED30021639

Overview
NameSMED30021639
Smed IDSMED30021639
Length (bp)3777
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30021639 (SMED30021639) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021639 (SMED30021639) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021639 (SMED30021639) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021639 (SMED30021639) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021639

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30021639SMESG000074722.1 SmedASXL_014757SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
germ cellSMED30021639SMESG000074722.1 Contig31883newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
germ cellSMED30021639SMESG000074722.1 Contig31883uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
zeta neoblastSMED30021639SMESG000074722.1 dd_Smed_v4_21438_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30021639SMESG000074722.1 SMED_09578_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30021639SMESG000074722.1 SMED_09578_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30021639 vs. Planmine SMEST
Match: SMESG000074722.1 (SMESG000074722.1)

HSP 1 Score: 493.041 bits (1268), Expect = 0.000e+0
Identity = 273/276 (98.91%), Postives = 275/276 (99.64%), Query Frame = 2
Query: 2165 KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMXXXXXXXXXXXXXXXDHCVLKNSVQTSHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSSMLEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCHLKNELQNELQNSRISFHDECENMEFYXXXXXXXXXXXXXXEKDFQPTKIRF 2992
            KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMENIKLKKNLKIKECCDHCVLKNSVQ SHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSS+LEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCHLKNELQNELQNSRISFHDECENMEFYTSTPFTSKTSSKNKEKDFQPTKI+F
Sbjct:  384 KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMENIKLKKNLKIKECCDHCVLKNSVQISHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSSILEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCHLKNELQNELQNSRISFHDECENMEFYTSTPFTSKTSSKNKEKDFQPTKIQF 659          

HSP 2 Score: 196.823 bits (499), Expect = 0.000e+0
Identity = 102/106 (96.23%), Postives = 104/106 (98.11%), Query Frame = 1
Query: 1849 LDDSSNSQNGSHINLSLEENNQQIKFLQQQNEDLKKQLINAQQIISTNILPEFVIKALSETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQ 2166
             DDSSNSQNGSHINLSL+ENNQQIKFLQQQNEDLKKQLINAQQIIST ILPEFVIKAL+ETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQ
Sbjct:  278 FDDSSNSQNGSHINLSLKENNQQIKFLQQQNEDLKKQLINAQQIISTKILPEFVIKALNETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQ 383          

HSP 3 Score: 539.265 bits (1388), Expect = 2.880e-177
Identity = 275/279 (98.57%), Postives = 276/279 (98.92%), Query Frame = 2
Query:  218 MSLISINNSYLLNKLTNTNANRETSLEMSFSSKHFQSTPIRKIFHKHVSFECPIRTSNDRQNHPKVLFHKPDNYQRNFEQSSEVEVLSKVDEFCDSMKDYVKSVLIDKEQYRTLAVQIDDAYMEIKDPHSVFTPPNDPISRFNYIINILQQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKIQCYXXXXXXXXXXXXPFDKEAIETIVTEYLEIFMNRENWNVSDIDNTLFHRYLLKVKQLIESLANANPNQFG 1054
            MSLISINNSYLLNKLTNTNANRETSLEMSFSSKHFQSTPIRKIFHKHVSFECPIRTSNDRQNHPKVLFHKPDNYQRNFEQSSEVEVLSKVDEFCDSMKDYVKSVLIDKEQYRTLAVQIDDAYME+KDPHSVFTPPNDPISRFNYIINI QQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKIQCYEIDEEDEMELIDPFDKEAIETIVTEYLEIFMNREN NVSDIDNTLFHRYLLKVKQLIESLANANPNQF 
Sbjct:    1 MSLISINNSYLLNKLTNTNANRETSLEMSFSSKHFQSTPIRKIFHKHVSFECPIRTSNDRQNHPKVLFHKPDNYQRNFEQSSEVEVLSKVDEFCDSMKDYVKSVLIDKEQYRTLAVQIDDAYMEVKDPHSVFTPPNDPISRFNYIINIFQQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKIQCYEIDEEDEMELIDPFDKEAIETIVTEYLEIFMNRENGNVSDIDNTLFHRYLLKVKQLIESLANANPNQFD 279          
BLAST of SMED30021639 vs. Planmine SMEST
Match: SMESG000074722.1 (SMESG000074722.1)

HSP 1 Score: 467.233 bits (1201), Expect = 0.000e+0
Identity = 247/251 (98.41%), Postives = 248/251 (98.80%), Query Frame = 2
Query: 2165 KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMXXXXXXXXXXXXXXXDHCVLKNSVQTSHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSSMLEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCHLKNELQNELQNSRISFHDECENMEFY 2917
            KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMENIKLKKNLKIKECCDHCVLKNSVQ SHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSS+LEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCH  NELQNELQNSRISFHDECENMEFY
Sbjct:  384 KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMENIKLKKNLKIKECCDHCVLKNSVQISHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSSILEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCH--NELQNELQNSRISFHDECENMEFY 632          

HSP 2 Score: 197.208 bits (500), Expect = 0.000e+0
Identity = 102/106 (96.23%), Postives = 104/106 (98.11%), Query Frame = 1
Query: 1849 LDDSSNSQNGSHINLSLEENNQQIKFLQQQNEDLKKQLINAQQIISTNILPEFVIKALSETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQ 2166
             DDSSNSQNGSHINLSL+ENNQQIKFLQQQNEDLKKQLINAQQIIST ILPEFVIKAL+ETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQ
Sbjct:  278 FDDSSNSQNGSHINLSLKENNQQIKFLQQQNEDLKKQLINAQQIISTKILPEFVIKALNETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQ 383          

HSP 3 Score: 538.11 bits (1385), Expect = 3.313e-176
Identity = 275/279 (98.57%), Postives = 276/279 (98.92%), Query Frame = 2
Query:  218 MSLISINNSYLLNKLTNTNANRETSLEMSFSSKHFQSTPIRKIFHKHVSFECPIRTSNDRQNHPKVLFHKPDNYQRNFEQSSEVEVLSKVDEFCDSMKDYVKSVLIDKEQYRTLAVQIDDAYMEIKDPHSVFTPPNDPISRFNYIINILQQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKIQCYXXXXXXXXXXXXPFDKEAIETIVTEYLEIFMNRENWNVSDIDNTLFHRYLLKVKQLIESLANANPNQFG 1054
            MSLISINNSYLLNKLTNTNANRETSLEMSFSSKHFQSTPIRKIFHKHVSFECPIRTSNDRQNHPKVLFHKPDNYQRNFEQSSEVEVLSKVDEFCDSMKDYVKSVLIDKEQYRTLAVQIDDAYME+KDPHSVFTPPNDPISRFNYIINI QQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKIQCYEIDEEDEMELIDPFDKEAIETIVTEYLEIFMNREN NVSDIDNTLFHRYLLKVKQLIESLANANPNQF 
Sbjct:    1 MSLISINNSYLLNKLTNTNANRETSLEMSFSSKHFQSTPIRKIFHKHVSFECPIRTSNDRQNHPKVLFHKPDNYQRNFEQSSEVEVLSKVDEFCDSMKDYVKSVLIDKEQYRTLAVQIDDAYMEVKDPHSVFTPPNDPISRFNYIINIFQQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKIQCYEIDEEDEMELIDPFDKEAIETIVTEYLEIFMNRENGNVSDIDNTLFHRYLLKVKQLIESLANANPNQFD 279          
BLAST of SMED30021639 vs. Planmine SMEST
Match: SMESG000074722.1 (SMESG000074722.1)

HSP 1 Score: 466.077 bits (1198), Expect = 1.313e-148
Identity = 247/251 (98.41%), Postives = 248/251 (98.80%), Query Frame = 2
Query: 2165 KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMXXXXXXXXXXXXXXXDHCVLKNSVQTSHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSSMLEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCHLKNELQNELQNSRISFHDECENMEFY 2917
            KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMENIKLKKNLKIKECCDHCVLKNSVQ SHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSS+LEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCH  NELQNELQNSRISFHDECENMEFY
Sbjct:  389 KTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEGNIEKCVMENIKLKKNLKIKECCDHCVLKNSVQISHKCVGISWQCIDSETASQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNATARLQSQIIAKRQQSPVSSILEKSNYFSTKSNFLQDNSVLCKTTDLAQSIEENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCH--NELQNELQNSRISFHDECENMEFY 637          

HSP 2 Score: 303.523 bits (776), Expect = 1.456e-88
Identity = 160/174 (91.95%), Postives = 164/174 (94.25%), Query Frame = 2
Query:  533 IDKEQYRTLAVQIDDAYMEIKDPHSVFTPPNDPISRFNYIINILQQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKIQCYXXXXXXXXXXXXPFDKEAIETIVTEYLEIFMNRENWNVSDIDNTLFHRYLLKVKQLIESLANANPNQFG 1054
            +++ +Y  L  QIDDAYMEIKDPHSVFTPPNDPISRFNYIINILQQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQK QCYEIDEEDEMELIDPFDKEAIETIVTEYLEIFMNREN NVSDIDNTLFHRYLLKVKQLIESLANANPN   
Sbjct:  111 LNENRYMKLVEQIDDAYMEIKDPHSVFTPPNDPISRFNYIINILQQPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETSNNLKQKFQCYEIDEEDEMELIDPFDKEAIETIVTEYLEIFMNRENGNVSDIDNTLFHRYLLKVKQLIESLANANPNHLD 284          

HSP 3 Score: 198.364 bits (503), Expect = 1.660e-52
Identity = 106/117 (90.60%), Postives = 110/117 (94.02%), Query Frame = 1
Query: 1849 LDDSSNSQNGSHINLSLEENNQQIKFLQQQNEDLKKQLINAQQIISTNILPEFVIKALSETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQNRK*IFSFKER 2199
            LDDSSNSQNGSHINLSL+ENNQQIKFLQQQNEDLKKQLINAQQIIST ILPEFVIKAL+ETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQ  +  FS  +R
Sbjct:  283 LDDSSNSQNGSHINLSLKENNQQIKFLQQQNEDLKKQLINAQQIISTKILPEFVIKALNETKELTLKEISEIDEKFSSERLSIEKHYEEKLDEYRQQLEKQDEEMQKTENEFSALKR 399          
The following BLAST results are available for this feature:
BLAST of SMED30021639 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021639 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000074722.12.880e-17798.91SMESG000074722.1[more]
SMESG000074722.13.313e-17698.41SMESG000074722.1[more]
SMESG000074722.11.313e-14898.41SMESG000074722.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021639 ID=SMED30021639|Name=SMED30021639|organism=Schmidtea mediterranea sexual|type=transcript|length=3777bp
ATGATCTTTAAAACTACGAATTTAATTTAGATACTATATTTCAGAAATAA
AAACAACATTTTTATGTAATCTATTGAAGCAAGTATTCCAATTTTACCAC
CAGTTTCACTATTTTTTATTACTTTATGTTAGTTACATAATAAAATTAAA
TACCCAAATTTTTATATTTAAAAATTGTTTGTTTAAAAATAACAAATTAT
GCATTCTGTTTTTACAAATGAGTTTGATTAGTATTAACAATTCATATCTT
TTAAATAAATTGACCAATACAAATGCTAACCGTGAAACTTCTTTGGAAAT
GTCATTTTCATCAAAACATTTTCAAAGTACGCCAATTAGAAAAATATTTC
ATAAGCATGTATCTTTTGAATGTCCAATACGAACATCAAATGATAGACAA
AACCACCCAAAAGTTTTATTTCATAAACCTGATAATTATCAAAGAAATTT
TGAACAATCTTCCGAAGTCGAAGTTTTATCCAAAGTTGATGAATTTTGTG
ATTCCATGAAAGATTATGTTAAAAGCGTACTTATAGACAAAGAACAATAT
AGAACGTTGGCTGTGCAAATTGATGATGCTTATATGGAAATAAAAGATCC
TCATTCTGTATTTACTCCTCCAAATGATCCAATTAGCCGCTTCAATTATA
TAATAAATATCCTTCAACAGCCAGTGGTTAAAGAACTGATGGTTAAATGT
TCAGAGAAAGAAGAAATTATTCAGAATTTACAAGGAGAACGAGGAAATTT
ATTGAAATGCACTGATAGTCTTTCTGTAATTTTTACATCTTTCGTTTCTT
GGGTAATTGAAACGTCGAACAATTTAAAACAGAAAATTCAATGTTATGAA
ATAGACGAGGAAGATGAAATGGAACTCATAGATCCATTTGATAAAGAAGC
TATTGAAACGATTGTGACGGAATACCTTGAAATATTTATGAATAGAGAAA
ATTGGAATGTATCAGACATTGATAACACTCTCTTTCACCGTTATCTATTG
AAAGTCAAACAGTTAATCGAATCACTGGCGAACGCAAATCCAAATCAGTT
TGGTAGTTAATTTTTGTGTCATCTTATCTTTGTGAATTCTTCTTTTGTCC
TTTTCTCATTTATTATCGAATGTTTCTAAAGAGCTTGGTAACATAAATGA
GCCAAACGATGTGATGACTTTAAAATGTAAAATATTCAATAGTATAGTTT
GGGTGATCCTAATGTAGCTCCATAAACATGAATCAGAACGGATTTTATCC
TTATATCTTGTTTGGAAATGTCACTTAAGAATCTTAAGTTAAGATGTAAG
CGGTAACAATCGCAATAAAACAAATCGGTGGTCATTATCTAGCAAGATTC
GTCAAAATTAATAATAAAAACATTCTGACCCACTCCACATTATGCGACTT
GCAAAATTATGCTTTTACATTTCCCTGGTCGATTAACATGCTAAATAATA
ATCCGTGTCTCCCAGTCTCTCAAAGCTCAAAACTTTGATAATTTAACTAA
TATATATATTACTGACAAATGAGAGTAGTTTTTTTTAATAATATTAAGAA
TGTTCAAAAAGGTTTGAAAATAAAATCATATTTCAAATCTTTATTGGAAA
TGCATAATTGGATTAAACAAAACTACTCTTGATAATCAGTAATATTTTCG
TGCCGCGACAGACAGACTAGCGGAATATAAGATGCATTCTGTCTATAATT
ATTCGATATCAATAAAATCCATAATTAATTTATAACTTATTAAATGTTAA
TTTTTATTTAAATTTAATCATTACTGCATATTATTATAACATGCATTAAA
TTTCAATGGATAGCATCGACGCAAACGGATTAAATATAACTATTTAATTT
AGATGATAGTTCAAATTCTCAAAATGGGTCTCATATAAATCTAAGTTTGG
AAGAAAACAATCAACAAATAAAATTTCTCCAACAACAAAACGAAGATCTT
AAAAAGCAACTGATAAATGCCCAGCAGATTATATCAACAAATATTCTTCC
CGAATTCGTTATAAAAGCACTGAGTGAGACCAAAGAATTGACTTTGAAAG
AAATATCAGAAATTGACGAAAAGTTTTCCAGTGAAAGATTGAGCATTGAA
AAACACTACGAAGAAAAACTAGATGAATATAGACAACAATTGGAAAAGCA
AGACGAGGAAATGCAAAACAGAAAATGAATTTTCAGCTTTAAAGAGAGAC
TTGAAATTTGTTGAAAAAAAATATGAAGATTTCGAAAAGTATCACAATGA
AATATTAAGGGATTTTGAGGGAAATATTGAAAAATGTGTTATGGAAAATA
TTAAATTGAAAAAGAATTTGAAAATCAAAGAATGCTGTGATCATTGTGTA
TTGAAAAATTCTGTACAAACATCTCATAAATGTGTCGGTATCAGCTGGCA
ATGCATCGATAGTGAAACCGCCAGTCAAATTGATGTGAATATAACGGACA
CTTGTCAAGAGAAAGTTGTTGAAGATTGTCTAGCTGAAATTGCAGATTTA
AAGAATTACGTACAAGTTCTGCAAGAAGTTATCACAAACCAACAAAATAC
AATTGATAGTTGCAATGCCACTGCGCGATTGCAGAGTCAAATAATAGCCA
AACGACAGCAGTCCCCAGTTAGCAGCATGCTTGAAAAATCGAATTATTTC
TCCACTAAATCGAATTTTCTGCAAGACAATTCTGTGCTATGTAAAACGAC
GGATCTGGCCCAATCTATAGAAGAGAATATATCTCCAGCAGATAATAATT
TTAATTCCTTTAAATTATTAAGAAACCCAAATTGTTCCAAGTTATCAACG
AGTCAAACTCACTATAATGCCGCTCAGAATCATTGCCATTTAAAGAATGA
ACTTCAAAATGAACTTCAAAATTCCAGAATTTCATTTCATGATGAATGTG
AAAATATGGAATTTTACACCTCAACGCCGTTCACAAGCAAAACCTCTAGT
AAAAATAAGGAAAAAGATTTTCAACCTACCAAGATTCGTTTTTGAGAATA
AATTTTTAGATGCCTTCTAGAAAGAAATTTCGAAATATATAGTCATTAAA
TAAACAAGTCAACGATGTCAGAAGGCTGCTTAAAATTTTCGGAACTGCTT
GTAGTTACACCCATTTGAAATAAAAAATAACATCCAATATCAGTAAGAAG
AATATTTGAAATTATATAGCAAAACAAGATGGACCCTTTAATCTATGCGG
AGATTTTAAAAAGAAACAACATAGTAGTTTCATTGTAACAATGTATAAGC
AGAATTTAATCCACTACAAGAAGTCTTGATTACATATTTAAATTATTGTA
GTTTTTTGCATTTTTCAGTTAACTTACTTTTATGAAGTTTTTGTTGAAAT
AAATTATACGTAACGCTTAATTCTCTAAAATATGGCTTTTTCACAAATTT
AAAATGTCAGGGTATGTGTGCTACACATTCTTAATTGTAATATTAGCTCT
TTATATAGTTTACATACTTTTACAAAAATTTCACCGTTATAAAGTATTAG
TAAAATTTTATTCTATATAATATGACTTGATTTGCTATTAAAAAATAGTG
AGTTGTGTGTGCTACGTATGATTTGTAGTGAATTATGAATTTTCAAGTAG
CTACCATAAGTTTATGTTTTCACTGTATCTAAGTTTAAGTATAAGTTAAC
TTCCTATAATATGGTTTGATTCGAAATTTAAAATAGTGTGTTGTGTGTAT
TACTACACATTTTCGTGTGAAATGTGTGATTTTTACATAGTTACTGTTAT
AGGATTTTATATGAAGATTTGAAAGGT
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protein sequence of SMED30021639-orf-1

>SMED30021639-orf-1 ID=SMED30021639-orf-1|Name=SMED30021639-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=281bp
MSLISINNSYLLNKLTNTNANRETSLEMSFSSKHFQSTPIRKIFHKHVSF
ECPIRTSNDRQNHPKVLFHKPDNYQRNFEQSSEVEVLSKVDEFCDSMKDY
VKSVLIDKEQYRTLAVQIDDAYMEIKDPHSVFTPPNDPISRFNYIINILQ
QPVVKELMVKCSEKEEIIQNLQGERGNLLKCTDSLSVIFTSFVSWVIETS
NNLKQKIQCYEIDEEDEMELIDPFDKEAIETIVTEYLEIFMNRENWNVSD
IDNTLFHRYLLKVKQLIESLANANPNQFGS*
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protein sequence of SMED30021639-orf-2

>SMED30021639-orf-2 ID=SMED30021639-orf-2|Name=SMED30021639-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=291bp
MNIDNNWKSKTRKCKTENEFSALKRDLKFVEKKYEDFEKYHNEILRDFEG
NIEKCVMENIKLKKNLKIKECCDHCVLKNSVQTSHKCVGISWQCIDSETA
SQIDVNITDTCQEKVVEDCLAEIADLKNYVQVLQEVITNQQNTIDSCNAT
ARLQSQIIAKRQQSPVSSMLEKSNYFSTKSNFLQDNSVLCKTTDLAQSIE
ENISPADNNFNSFKLLRNPNCSKLSTSQTHYNAAQNHCHLKNELQNELQN
SRISFHDECENMEFYTSTPFTSKTSSKNKEKDFQPTKIRF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000227germ cell
PLANA:0000429neoblast
PLANA:0002109X1 cell