SMED30021638

Overview
NameSMED30021638
Smed IDSMED30021638
Length (bp)1152
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30021638 (SMED30021638) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021638 (SMED30021638) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021638 (SMED30021638) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021638 (SMED30021638) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021638

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30021638h1SMcG0017360 SmedASXL_009231SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30021638h1SMcG0017359 SmedASXL_009231SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30021638h1SMcG0017360 Contig18481newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30021638h1SMcG0017360 Contig18481uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30021638h1SMcG0017361 Contig18481newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30021638h1SMcG0017361 Contig18481uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30021638 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004240:17138..30552 -5507
Homology
[Close]
BLAST of SMED30021638 vs. Ensembl Celegans
Match: F13E6.2 (pep chromosome:WBcel235:X:10668280:10674293:-1 gene:WBGene00008746.1 transcript:F13E6.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F13E6.2)

HSP 1 Score: 53.5286 bits (127), Expect = 1.545e-7
Identity = 49/225 (21.78%), Postives = 102/225 (45.33%), Query Frame = 2 
Query:  419 SNAVMNAAQL-YIVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHYDFASI-----------QPFDICPVSRK-------KSKVNCCIVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQDPEKLSKQCIDVLRSVFY 1036
            S+ +++AA+L   V +P N        P+I + GAP   K  +++ I +   G+       I           + +D   VS+K        +K+   ++ + L   G+ + G ++E +PKS   L     ++ + DL I ID ++    +  +  +N + K+      +  +  V++ +EY ++  LP ++   DK     ++G+ DP+ + K+ + V+    Y
Sbjct:  494 SSEIVSAAELGEPVGLPNN-------APVILVLGAPGSQKNDISRRIAQKYDGFTMLSMGDILRKKINNEKNDEMWDK--VSKKMNNGDPIPTKMCRTVLYEELHSRGTSNWGYVIEGYPKSPDQLVDLEHSLQRTDLAILIDCTE-QFCLEVINKRNRENKR-----SDDDSDAVRSRMEYFKKNTLPMLKTLDDKGKLRVVDGDADPDTVFKEVVQVIDRTLY 703
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BLAST of SMED30021638 vs. TrEMBL
Match: A0A5J4NBT0 (Adenylate kinase OS=Paragonimus westermani OX=34504 GN=DEA37_0002617 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.050e-7
Identity = 50/225 (22.22%), Postives = 95/225 (42.22%), Query Frame = 2 
Query:  428 VMNAAQLYIVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHY---DFASIQPFDICPVSRKKS-----------------KVNCCIVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQDPEKLSKQCIDVLRSVFYGK 1042
            V N  ++    V K R  +L   P+I + G P  G+T  A+ +C+ LPG  H+   +F   +  D      +K                   V   I+ +  + +   +  +I+E FP     L  F++ IG  DL I +D  +  +  R L+    +  ++      ++ +L + +  + +   LP VR++ ++ +   I G++D E +    + V    F GK
Sbjct:  340 VFNIPRIVPTFVDKGRVADLHTCPVILLFGGPGSGRTEQAQALCERLPGLKHFSVTEFLRKRVLDHIEDDEQKDWDVVARRVHSSDPPMIKDVQVDILRQEFDSVAEDAKAVIIEGFPNDGNQLNTFNQHIGGADLAILLDCEESTLQKRLLK----RYARLSRTEDEETVALHRII--FFKHCTLPVVRHYDERSILVTIPGDRDRELVLNDVVTVA-EYFLGK 557
[Close]
BLAST of SMED30021638 vs. TrEMBL
Match: A0A4E0RMN6 (Adenylate kinase isoenzyme 5 OS=Fasciola hepatica OX=6192 GN=D915_007311 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.914e-7
Identity = 49/212 (23.11%), Postives = 94/212 (44.34%), Query Frame = 2 
Query:  440 AQLYIVNVP--------KNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHY---DFA-----------SIQPFDIC--------PVSRKKSKVNCCIVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQD 985
             Q  + N+P        K R  +L   P+I + G P  G+T  A+ +C  L G  H+   DF            S + +D+         P +++ ++V+  I+ +  E + + S  +++E FP  E  L  F++ IG  DL I +D  +  +  R L     +  ++G        SL + +  + +   LP VR++ ++ +   I G+++
Sbjct:  331 GQPPVFNIPRIVPQFPDKGRVADLYTCPVILLFGGPGSGRTEQAQALCGRLAGLKHFNVTDFLRQRVLYHIEEDSAKDWDVVARRVHSSDPPNKRDTQVD--IIRQEFEGVANKSRAVVIEGFPNDENQLNTFNQHIGGADLAILLDCEESTLQKRLLS----RYTRLGRMEDEDMVSLQRIL--FFKNCTLPVVRHYDERSILVTIPGDRE 534
[Close]
BLAST of SMED30021638 vs. TrEMBL
Match: H2KP93 (Adenylate kinase isoenzyme 5 OS=Clonorchis sinensis OX=79923 GN=CLF_101549 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 7.357e-7
Identity = 48/231 (20.78%), Postives = 98/231 (42.42%), Query Frame = 2 
Query:  440 AQLYIVNVPK--------NRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHYDFA--------------SIQPFDICPVSRK--------KSKVNCCIVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQDPEKLSKQCIDVLRSVFYGK 1042
             Q  + N+P+         R   L   P+I + G P  G++  A+ +C+ LPG  H++                S + +D+  V+R+           V   I+ +    +   +  +++E FP  ET L  F++ IG  DL I +D  +  +  R L+    +  ++      ++ +L + +  + +   LP +R++ ++     I  ++D E +    + V+   F GK
Sbjct:  195 GQPPVFNIPRITPAFADNGRVEGLPTCPIILLFGGPGSGRSEQARALCEKLPGLKHFNVTEFLRNRVLDHIEGDSQKDWDV--VARRVHSSDPPMPKNVQVDILRQEFNTVAENTKAVVIEGFPNDETQLNIFNQHIGGADLAILLDCEENTLQKRLLK----RYTRLSRTEDEETVALHRIL--FFKHCTLPVIRHYDERSTLVTIPADRDQELVLNDVVTVV-EYFLGK 416
[Close]
BLAST of SMED30021638 vs. TrEMBL
Match: A0A210QMB2 (Adenylate kinase isoenzyme 5 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT03819 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.823e-6
Identity = 49/212 (23.11%), Postives = 94/212 (44.34%), Query Frame = 2 
Query:  452 IVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHYDFASIQPFDICP--VSRKKSKVNCCIVGKALEVIGSFSV--------------GIILENFPKSETDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQDPEKLSKQCIDVLRSVFYG 1039
            IVN  + R   L   P+I + G P  GK T AK I +  PG  H     I   +I     + +K  +   +V +        +V               IILE +P+ +T ++ F++ IG ++  + ID+ ++ +  R L  +  + +++  NL     S + + + + R   LP ++++ D      + G++D +++      VL   F+G
Sbjct:  368 IVNKDEGRKPELPTCPIIVLAGGPGSGKGTQAKMIIERYPGTVHVSMGDILRTEISQKGTADQKWGMISSLVQQGEMAPEEVTVDLLKDNLAKHKDASAIILEGYPRDKTQVEEFNKYIGGLNFVLLIDSEEYYMQKR-LVDRGKETERIDDNL-----SAISSRINFFRNKTLPVLKHYDDNGKLVALYGDRDIQEVFFDVTQVLDKAFFG 573
[Close]
BLAST of SMED30021638 vs. Planmine SMEST
Match: SMESG000053924.1 (SMESG000053924.1)

HSP 1 Score: 220.705 bits (561), Expect = 2.346e-68
Identity = 109/110 (99.09%), Postives = 110/110 (100.00%), Query Frame = 2 
Query:  242 NETDILNIVKKITDLVSNESPEIINKYRNDLIKAEEFLNNLLITKSKTNDGSLHFVHSSSNAVMNAAQLYIVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALP 571
            NETDILNIVKKITDLVSNESPEIINKYRNDLIKAEEFLNNLLITKSKTNDGSLHFVHSSSNAVMNAAQLYIV+VPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALP
Sbjct:  264 NETDILNIVKKITDLVSNESPEIINKYRNDLIKAEEFLNNLLITKSKTNDGSLHFVHSSSNAVMNAAQLYIVDVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALP 373
[Close]
BLAST of SMED30021638 vs. Planmine SMEST
Match: SMESG000024490.1 (SMESG000024490.1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.443e-15
Identity = 57/232 (24.57%), Postives = 100/232 (43.10%), Query Frame = 2 
Query:  368 ITK-SKTNDGSLHFVHSSSNAVMNAAQLYIVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHYDFASIQPFDICPVSRKKSKVNCC--------------------------IVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQ 982
            ITK  KT D            V +  +++ ++    R  +L + P++ + G P CGKTT A EIC     W H +   I  + I     + +  +C                            + + L+ + S + GIILE FPK +  LQ F+  IG+ DL I +D  ++ ++ R L  +  ++ +   +L N     +K  + Y +   LP  RY+ ++ +   I G++
Sbjct:  272 ITKIKKTEDKYNEIPPPGVAQVQDIEEIFNIDPEIIRPSDLPQCPIVILLGGPGCGKTTAATEICTRYKDWVHINVNDIIRYRIFDKGVESTNWDCLAKLSLLNDVHLDQNQLVIEHWNINVEFIKEELKTLKSSTQGIILEGFPKDKVQLQQFNIEIGRADLVILLDCDEYYMTTRLL-ERGERLHRADDDLSN-----IKKRIIYYKTSGLPVCRYYDERHILNIIPGDR 497
[Close]
BLAST of SMED30021638 vs. Planmine SMEST
Match: SMESG000024490.1 (SMESG000024490.1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.839e-15
Identity = 50/196 (25.51%), Postives = 87/196 (44.39%), Query Frame = 2 
Query:  473 RSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHYDFASIQPFDICPVSRKKSKVNCC--------------------------IVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQ 982
            R  +L + P++ + G P CGKTT A EIC     W H +   I  + I     + +  +C                            + + L+ + S + GIILE FPK +  LQ F+  IG+ DL I +D  ++ ++ R L  +  ++ +   +L N     +K  + Y +   LP  RY+ ++ +   I G++
Sbjct:  292 RPSDLPQCPIVILLGGPGCGKTTAATEICTRYKDWVHINVNDIIRYRIFDKGVESTNWDCLAKLSLLNDVHLDQNQLVIEHWNINVEFIKEELKTLKSSTQGIILEGFPKDKVQLQQFNIEIGRADLVILLDCDEYYMTTRLL-ERGERLHRADDDLSN-----IKKRIIYYKTSGLPVCRYYDERHILNIIPGDR 481
[Close]
BLAST of SMED30021638 vs. Planmine SMEST
Match: SMESG000020480.1 (SMESG000020480.1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.080e-10
Identity = 54/211 (25.59%), Postives = 94/211 (44.55%), Query Frame = 2 
Query:  443 QLYIVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHYDFASI-----------QPFDICPVSRKKSK---------VNCCIVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSD----FNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYF----------SDKDVSFNIN 973
            +L  + +   R  +L +  LI + G P  GKT  A ++CK  P W H D   I            P DI   + + +          VN   + K L+ +   +  IILE +PK++ +L   +  IGKID  I +D+ +    + ++ R+ R  +   + +GSN  N+        ++Y +   LP  RY+          SD+D+++ I+
Sbjct:  175 ELTGIAIETARKDDLPQCTLILLLGGPGSGKTETAIQLCKRYPNWVHIDMNDILRRCMVQNGLSDP-DIAQTADENNPESFIVENLIVNINALKKHLKSVDDCAQVIILEGYPKNKIELNDLNTEIGKIDFVILLDSEEDVMKYRLNERWSRFGS--KEDIGSNSENR--------IQYFKTNGLPLCRYYDERCQLNIVNSDRDIAYVID 374
[Close]
BLAST of SMED30021638 vs. Planmine SMEST
Match: SMESG000020480.1 (SMESG000020480.1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.097e-10
Identity = 54/211 (25.59%), Postives = 94/211 (44.55%), Query Frame = 2 
Query:  443 QLYIVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWFHYDFASI-----------QPFDICPVSRKKSK---------VNCCIVGKALEVIGSFSVGIILENFPKSETDLQFFHETIGKIDLQIFIDTSD----FNISMRYLRSQNIKVKKVGSNLGNKSASLVKAVLEYNRQIILPTVRYF----------SDKDVSFNIN 973
            +L  + +   R  +L +  LI + G P  GKT  A ++CK  P W H D   I            P DI   + + +          VN   + K L+ +   +  IILE +PK++ +L   +  IGKID  I +D+ +    + ++ R+ R  +   + +GSN  N+        ++Y +   LP  RY+          SD+D+++ I+
Sbjct:  185 ELTGIAIETARKDDLPQCTLILLLGGPGSGKTETAIQLCKRYPNWVHIDMNDILRRCMVQNGLSDP-DIAQTADENNPESFIVENLIVNINALKKHLKSVDDCAQVIILEGYPKNKIELNDLNTEIGKIDFVILLDSEEDVMKYRLNERWSRFGS--KEDIGSNSENR--------IQYFKTNGLPLCRYYDERCQLNIVNSDRDIAYVID 384
The following BLAST results are available for this feature:
BLAST of SMED30021638 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
F13E6.21.545e-721.78pep chromosome:WBcel235:X:10668280:10674293:-1 gen... [more]
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BLAST of SMED30021638 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
A0A5J4NBT02.050e-722.22Adenylate kinase OS=Paragonimus westermani OX=3450... [more]
A0A4E0RMN64.914e-723.11Adenylate kinase isoenzyme 5 OS=Fasciola hepatica ... [more]
H2KP937.357e-720.78Adenylate kinase isoenzyme 5 OS=Clonorchis sinensi... [more]
A0A210QMB21.823e-623.11Adenylate kinase isoenzyme 5 OS=Mizuhopecten yesso... [more]
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BLAST of SMED30021638 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021638 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000053924.12.346e-6899.09SMESG000053924.1[more]
SMESG000024490.17.443e-1524.57SMESG000024490.1[more]
SMESG000024490.17.839e-1525.51SMESG000024490.1[more]
SMESG000020480.11.080e-1025.59SMESG000020480.1[more]
SMESG000020480.11.097e-1025.59SMESG000020480.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021638 ID=SMED30021638|Name=SMED30021638|organism=Schmidtea mediterranea sexual|type=transcript|length=1152bp
AATATATAAAAGGTATTGATACTAATCTGATACAATGAATTCTCAAATTT
AATCGCAATTCACAATTCTGAACTGATTTGATTTAAATTCATTTATTCCT
ATTCAAAGGCAGCGCCATACTATCTCATGTGCAGCAAGGGAAATTTCAAA
TGTGCAATATTTTTGATATAAAATACACTAGAAAATCTTCTTCAATAGTT
CATAAATATCACGAATTGAAAAAAAAATTTCACAGATAGAAAATGAAACA
GATATTTTAAATATCGTCAAGAAAATAACGGATTTAGTTAGCAATGAATC
GCCAGAAATCATAAATAAATACAGAAATGATTTGATTAAAGCTGAGGAAT
TTTTAAACAACCTGTTAATCACAAAATCAAAAACAAATGATGGCTCTCTC
CATTTTGTCCACAGTTCATCAAATGCTGTTATGAATGCTGCGCAACTTTA
CATAGTAAATGTACCGAAAAATAGGTCATATAATCTAAGCAGGGTTCCCT
TGATAACGATTCAAGGAGCCCCAAAATGCGGCAAGACTACTGTGGCAAAA
GAAATATGCAAAGCATTGCCAGGTTGGTTTCATTACGATTTTGCATCCAT
ACAGCCATTTGATATTTGCCCTGTCAGTAGAAAGAAGAGCAAAGTAAACT
GTTGCATCGTTGGAAAAGCTCTTGAAGTTATAGGGTCATTTTCTGTTGGG
ATTATTTTGGAAAATTTTCCGAAATCGGAAACTGATCTCCAGTTTTTTCA
CGAAACGATTGGGAAAATAGATCTCCAGATTTTTATCGACACATCCGACT
TCAATATCTCAATGCGATATCTTCGAAGCCAAAACATAAAAGTAAAAAAA
GTAGGAAGCAATTTAGGAAATAAAAGCGCTTCATTGGTGAAGGCAGTTTT
GGAATATAACAGACAAATAATATTGCCAACTGTCCGTTACTTTTCTGACA
AAGATGTGTCATTTAATATAAATGGAAATCAAGATCCAGAAAAGTTGTCT
AAGCAGTGCATCGATGTTCTTCGGTCAGTTTTCTATGGAAAATGACTTGA
ATATAATAAATCAGAATTCAAACAAACTAAACAAGTATTATCGAAACAGC
GACAGAAATAGGGAAATAATTTGCAGTGTTATTTGATAAAAAAATAATAA
AT
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protein sequence of SMED30021638-orf-1

>SMED30021638-orf-1 ID=SMED30021638-orf-1|Name=SMED30021638-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=205bp
MNAAQLYIVNVPKNRSYNLSRVPLITIQGAPKCGKTTVAKEICKALPGWF
HYDFASIQPFDICPVSRKKSKVNCCIVGKALEVIGSFSVGIILENFPKSE
TDLQFFHETIGKIDLQIFIDTSDFNISMRYLRSQNIKVKKVGSNLGNKSA
SLVKAVLEYNRQIILPTVRYFSDKDVSFNINGNQDPEKLSKQCIDVLRSV
FYGK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0016301kinase activity
GO:0016740transferase activity
GO:0019205nucleobase-containing compound kinase activity
Vocabulary: biological process
TermDefinition
GO:0006139nucleobase-containing compound metabolic process
GO:0016310phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 71..202
e-value: 4.4E-11
score: 44.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 23..70
e-value: 2.9E-5
score: 26.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 20..200