Conserved uncharacterized CXorf65-related protein

Overview
NameConserved uncharacterized CXorf65-related protein
Smed IDSMED30021615
Length (bp)565
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Conserved uncharacterized CXorf65-related protein (SMED30021615) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Conserved uncharacterized CXorf65-related protein (SMED30021615) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021615

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30021615SMESG000033459.1 SmedASXL_010458SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30021615SMESG000033459.1 dd_Smed_v4_15167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021615SMESG000033459.1 dd_Smed_v4_15167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021615SMESG000033459.1 dd_Smed_v4_15167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021615SMESG000033459.1 dd_Smed_v4_15167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021615SMESG000033459.1 Contig55846newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021615SMESG000033459.1 Contig55846uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30021615SMESG000033459.1 dd_Smed_v6_15167_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Conserved uncharacterized CXorf65-related protein vs. TrEMBL
Match: A0A2B4SMC2 (Uncharacterized protein CXorf65-like OS=Stylophora pistillata OX=50429 GN=Gm614 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 9.177e-15
Identity = 47/147 (31.97%), Postives = 79/147 (53.74%), Query Frame = 1 
Query:   67 FVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANP----YLPLLDNESLVTPSFLEQLXXXXXXXXXXXXELA---KKSHLKPSFEIKEESNRRKLS 486
            F+ V YG+ ++ + N +CR++ L++ +KR C       EN + DL D+ G +KN+ +    +G+E L+ RE  ++V+V  +        Y PLLD+E ++T  FLE L+R            +   ++  LKPS + K+ S  R LS
Sbjct:    2 FITVKYGDHREALFNPNCRIIHLLEDIKRRC----NCAENAVIDLSDLQGAVKNLTDHQASNGIEFLNERETFILVRVEKNIEGREFPLYTPLLDDEDVLTKDFLESLRRTPTDTLEVSGRGSRSMRRGSLKPSVKRKQSSGNRSLS 144
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BLAST of Conserved uncharacterized CXorf65-related protein vs. TrEMBL
Match: A7RXB3 (Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g203535 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.988e-10
Identity = 39/107 (36.45%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:   67 FVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTN--DAANP-YLPLLDNESLVTPSFLEQL 378
            F+ V YG  ++ + N +CR++ L+D +KR C    K TE +L DL DVNG ++N+ +    +  + L  RE  ++V+V        P Y PLL +   VT  FLE L
Sbjct:    2 FITVKYGEAEEALFNPNCRILHLLDDIKRRC----KCTEEVLVDLSDVNGSVQNLADNQSCNATDFLKGRETFILVRVEKIEGVERPIYTPLLRDSDYVTSEFLESL 104
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BLAST of Conserved uncharacterized CXorf65-related protein vs. TrEMBL
Match: V3ZYN8 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_204334 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.190e-8
Identity = 33/108 (30.56%), Postives = 57/108 (52.78%), Query Frame = 1 
Query:   64 TFVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTND---AANPYLPLLDNESLVTPSFLEQL 378
            +F+ V YG+ ++ I N  C    L++Y++R C       +  + DLVD  G ++N+   T++   E +  RE  ++V++  D    +N Y+PLLDN     P  L +L
Sbjct:   14 SFIRVKYGHDKEAIFNPWCSTYTLLEYIRRTC----DCDQEYVIDLVDQQGHVRNLSTGTEEYANELVSARETYVLVRIERDGEMGSNKYIPLLDNLDKFNPELLVKL 117
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BLAST of Conserved uncharacterized CXorf65-related protein vs. TrEMBL
Match: A0A2B4SYP5 (Uncharacterized protein CXorf65-like OS=Stylophora pistillata OX=50429 GN=Gm614 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.863e-8
Identity = 40/123 (32.52%), Postives = 65/123 (52.85%), Query Frame = 1 
Query:   67 FVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDA---ANPYLPLLDNESLVTPSFLEQLXXXXXXXXXXXXELAK 426
            F+ V YG+ ++ I N +CR   L++ +KR C      T++   DL D +G +K + N       E L  RE L++++V   +    + Y P+L++  +VTP FLE+L R +      K  LAK
Sbjct:    2 FITVRYGDGEESIFNPNCRTNILLEDIKRRC----NCTKDATVDLSDESGNLKYLANHPMSYATEILKERESLVLIRVEKKSEAEGDSYTPMLNDMIIVTPQFLERLSRCESDSLNAKNSLAK 120
[Close]
BLAST of Conserved uncharacterized CXorf65-related protein vs. TrEMBL
Match: A0A3M6UK83 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00013155 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.403e-8
Identity = 42/140 (30.00%), Postives = 72/140 (51.43%), Query Frame = 1 
Query:   88 NQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANP----YLPLLDNESLVTPSFLEQLXXXXXXXXXXXXELA---KKSHLKPSFEIKEESNRRKLS 486
            N ++ + N +CR++ L++ +KR C       EN + DL D+ G +KN+ +    +G E L+ RE  ++V+V  +        Y PLL++E ++T  FLE L+R            +   ++  LKPS + K+ S    LS
Sbjct:   10 NHEEALFNPNCRILHLLEDIKRRC----NCAENAVIDLSDLQGAVKNLTDHQASNGSEFLNERETFILVRVEKNIEGREFPLYTPLLNDEDVLTKDFLESLRRTPTDTLEVSGRGSRSMRRGSLKPSVKRKQSSGNLSLS 145
[Close]
BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Nematostella
Match: EDO43935 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXB3])

HSP 1 Score: 66.6254 bits (161), Expect = 2.750e-14
Identity = 39/107 (36.45%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:   67 FVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTN--DAANP-YLPLLDNESLVTPSFLEQL 378
            F+ V YG  ++ + N +CR++ L+D +KR C    K TE +L DL DVNG ++N+ +    +  + L  RE  ++V+V        P Y PLL +   VT  FLE L
Sbjct:    2 FITVKYGEAEEALFNPNCRILHLLDDIKRRC----KCTEEVLVDLSDVNGSVQNLADNQSCNATDFLKGRETFILVRVEKIEGVERPIYTPLLRDSDYVTSEFLESL 104
[Close]
BLAST of Conserved uncharacterized CXorf65-related protein vs. Planmine SMEST
Match: SMESG000033459.1 (SMESG000033459.1)

HSP 1 Score: 189.504 bits (480), Expect = 8.536e-63
Identity = 90/92 (97.83%), Postives = 91/92 (98.91%), Query Frame = 1 
Query:   61 MTFVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPYLPLL 336
            MTFVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPY+P L
Sbjct:    1 MTFVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPYVPFL 92
[Close]
BLAST of Conserved uncharacterized CXorf65-related protein vs. Planmine SMEST
Match: SMESG000033459.1 (SMESG000033459.1)

HSP 1 Score: 136.732 bits (343), Expect = 3.052e-42
Identity = 65/67 (97.01%), Postives = 66/67 (98.51%), Query Frame = 1 
Query:  136 MDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPYLPLL 336
            MDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPY+P L
Sbjct:    1 MDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPYVPFL 67
[Close]
BLAST of Conserved uncharacterized CXorf65-related protein vs. Planmine SMEST
Match: SMESG000023661.1 (SMESG000023661.1)

HSP 1 Score: 60.077 bits (144), Expect = 1.365e-11
Identity = 32/107 (29.91%), Postives = 57/107 (53.27%), Query Frame = 1 
Query:   67 FVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDVNGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPYL---PLLDNESLVTPSFLEQL 378
            FV++H+G+ +K I N DC  +  +DY+++NCL    I E+ +FD  D+NG +  I +   +S   +   R   + V +  D  N      PL++  + + PS  ++L
Sbjct:   11 FVWIHHGDNKKFIANTDCSALNFLDYIRKNCL----IDESDIFDFTDINGKLLQISDQFHESWHTQFENRSIYIPVSIDKDDHNEICDVRPLIEGINELYPSLQDKL 113
The following BLAST results are available for this feature:
BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2B4SMC29.177e-1531.97Uncharacterized protein CXorf65-like OS=Stylophora... [more]
A7RXB31.988e-1036.45Predicted protein OS=Nematostella vectensis OX=453... [more]
V3ZYN81.190e-830.56Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A2B4SYP51.863e-832.52Uncharacterized protein CXorf65-like OS=Stylophora... [more]
A0A3M6UK832.403e-830.00Uncharacterized protein OS=Pocillopora damicornis ... [more]
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO439352.750e-1436.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Conserved uncharacterized CXorf65-related protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000033459.18.536e-6397.83SMESG000033459.1[more]
SMESG000033459.13.052e-4297.01SMESG000033459.1[more]
SMESG000023661.11.365e-1129.91SMESG000023661.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021615 ID=SMED30021615|Name=Conserved uncharacterized CXorf65-related protein|organism=Schmidtea mediterranea sexual|type=transcript|length=565bp
TTAAACATTGAAAATAATTCTGTTAAATTCTGTTGGATAATTTGGAAGTA
TTCATAGAAAATGACATTCGTATTTGTGCACTATGGGAACCAACAGAAGA
TGATCCTCAACGCAGACTGCAGGCTCGTGTGTTTAATGGATTATTTGAAG
AGAAATTGTTTACAAAATTACAAAATAACAGAGAACATGCTCTTTGATCT
CGTGGACGTTAATGGAGGAATAAAGAATATCCGAAATATAACTGACCAAA
GTGGGTTAGAGGAATTACATACGAGAGAAGAATTATTAATTGTACAAGTA
ACAAACGATGCTGCTAATCCTTACTTACCTCTTCTTGACAATGAAAGTCT
TGTAACCCCCTCGTTTTTGGAACAACTGAAAAGAGCAGATAAAAGAAAAG
CAGCTCACAAAAAAGAATTAGCCAAAAAATCTCATTTAAAACCAAGTTTT
GAAATTAAAGAAGAATCAAATCGTCGTAAATTATCAGCTCACAAAAGAAA
GACCTAAAAATTTGACATCAAAAATATGTTAAAAAATATTCATTGTATTT
TAAATAATTGTTTAG
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protein sequence of SMED30021615-orf-1

>SMED30021615-orf-1 ID=SMED30021615-orf-1|Name=SMED30021615-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp
MTFVFVHYGNQQKMILNADCRLVCLMDYLKRNCLQNYKITENMLFDLVDV
NGGIKNIRNITDQSGLEELHTREELLIVQVTNDAANPYLPLLDNESLVTP
SFLEQLKRADKRKAAHKKELAKKSHLKPSFEIKEESNRRKLSAHKRKT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000418head
PLANA:0000420parapharyngeal region