RAD17 checkpoint clamp loader component

Overview
NameRAD17 checkpoint clamp loader component
Smed IDSMED30021612
Length (bp)1786
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of RAD17 checkpoint clamp loader component (SMED30021612) t-SNE clustered cells

Violin plots show distribution of expression levels for RAD17 checkpoint clamp loader component (SMED30021612) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RAD17 checkpoint clamp loader component (SMED30021612) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RAD17 checkpoint clamp loader component (SMED30021612) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30021612

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30021612h1SMcG0003515 SmedASXL_012250SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30021612h1SMcG0003515 SmedASXL_012250SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
parenchymaSMED30021612h1SMcG0003515 dd_Smed_v4_20423_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Human
Match: RAD17 (RAD17 checkpoint clamp loader component [Source:HGNC Symbol;Acc:HGNC:9807])

HSP 1 Score: 177.178 bits (448), Expect = 1.431e-47
Identity = 154/486 (31.69%), Postives = 225/486 (46.30%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWI-----QKQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W+     ++QP    S  ++  TGPPG GKT TL  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI IISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  +ID +    E  S++D L   W      L  +Y  S  TR +++ N +R         +++ PL K     I K   +  L       LFP F      L +  L ++ LPYL 
Sbjct:    6 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLI--TGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDW--NTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLA---AKALFPDF-----CLPALCLQTQLLPYLA 469
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Human
Match: RAD17 (RAD17 checkpoint clamp loader component [Source:HGNC Symbol;Acc:HGNC:9807])

HSP 1 Score: 176.407 bits (446), Expect = 2.171e-47
Identity = 154/486 (31.69%), Postives = 222/486 (45.68%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSET--LPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT TL  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI IISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  +ID +    E  S++D L   W      L  +Y  S  TR +++ N +R         +++ PL K     I K   +  L       LFP F       C  +L  +T  LPYL 
Sbjct:    6 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDW--NTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLA---AKALFPDF-------CLPALCRQTQLLPYLA 469
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Human
Match: RAD17 (RAD17 checkpoint clamp loader component [Source:HGNC Symbol;Acc:HGNC:9807])

HSP 1 Score: 176.792 bits (447), Expect = 4.849e-47
Identity = 153/484 (31.61%), Postives = 222/484 (45.87%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT TL  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI IISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  +ID +    E  S++D L   W      L  +Y  S  TR +++ N +R         +++ PL K     I K   +  L       LFP F      L +  L ++ LPYL 
Sbjct:   92 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDW--NTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLA---AKALFPDF-----CLPALCLQTQLLPYLA 555
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Human
Match: RAD17 (RAD17 checkpoint clamp loader component [Source:HGNC Symbol;Acc:HGNC:9807])

HSP 1 Score: 176.792 bits (447), Expect = 4.849e-47
Identity = 153/484 (31.61%), Postives = 222/484 (45.87%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT TL  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI IISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  +ID +    E  S++D L   W      L  +Y  S  TR +++ N +R         +++ PL K     I K   +  L       LFP F      L +  L ++ LPYL 
Sbjct:   92 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDW--NTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLA---AKALFPDF-----CLPALCLQTQLLPYLA 555
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Human
Match: RAD17 (RAD17 checkpoint clamp loader component [Source:HGNC Symbol;Acc:HGNC:9807])

HSP 1 Score: 176.792 bits (447), Expect = 4.849e-47
Identity = 153/484 (31.61%), Postives = 222/484 (45.87%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT TL  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI IISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  +ID +    E  S++D L   W      L  +Y  S  TR +++ N +R         +++ PL K     I K   +  L       LFP F      L +  L ++ LPYL 
Sbjct:   92 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDW--NTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLA---AKALFPDF-----CLPALCLQTQLLPYLA 555
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Celegans
Match: hpr-17 (Homolog of S. Pombe Rad; Rad17-like protein [Source:UniProtKB/TrEMBL;Acc:G5EGG2])

HSP 1 Score: 100.908 bits (250), Expect = 2.410e-22
Identity = 103/433 (23.79%), Postives = 200/433 (46.19%), Query Frame = 3 
Query:  171 DYLAVHSKKIQEVLNWIQKQ-PXXXXXXXXXXXTGPPGSGKTATLYAL---PNICIHCWNDETQSNIVEDF----------TDFLLKASRYSELNDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDS-QTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITM----QDNNSALVDMNKDNPIFIFRALGKILYSKRETDEL-EIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPK-NTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            D L +H+KKI EV +W++     ++    ++ LTGP GSGK+ T+  +    NI I  ++ E   N  EDF            FLL+  R+  L       + K+L+L+   P+Q YS+       L         P+I  +++S      +P  L  K       ID ++F P+A + MKKA+ +        +++ +L    N I E + GD+R A+N L++        + + ++++  +K N    F  +G+ILY+KR    + + +++ ++ ++ + + +   R +L  +P  +     +     +++L +N   F  +I +   + E FS  D+L   W   ++ L   Y+    TRS++++N   P+  T   + +   S ++K   +T+L +  +            ++ +K   + T PY+  I
Sbjct:   19 DELQIHNKKIAEVDHWLKNVFSESNKQLGVMYLTGPAGSGKSTTVEVMCTEQNIEIIEYSPEYLHN--EDFECEKPDFTQLRRFLLR--RHGSLR---GGGLKKRLLLVTELPDQAYSDAEKFREDLSEVLQHIWHPVIFCLTNSIACWNLNPDRLFTKDFNIMNGIDTVTFNPVADSFMKKALVRASNCLSSPLSDAKL----NVIGEEAGGDLRIAMNMLQMNSIGPNADRRSGNSVICASKANREEAFHMIGRILYAKRVNPNVPKPSRFSKRRRKSAPIPEPLVRTELEHDPTDIITMSSMTSEKLLDFLFQNEPIFCSNISKYRYVAETFSMCDFLTGDWTTRKS-LPEDYVAQMATRSVMWNNYKEPRPGTLFAVGRPLRSSLEKHTARTKLELQRLP-----------MIGAKDYAALTCPYITII 428
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Celegans
Match: hpr-17 (Homolog of S. Pombe Rad; Rad17-like protein [Source:UniProtKB/TrEMBL;Acc:G5EGG2])

HSP 1 Score: 100.908 bits (250), Expect = 2.410e-22
Identity = 103/433 (23.79%), Postives = 200/433 (46.19%), Query Frame = 3 
Query:  171 DYLAVHSKKIQEVLNWIQKQ-PXXXXXXXXXXXTGPPGSGKTATLYAL---PNICIHCWNDETQSNIVEDF----------TDFLLKASRYSELNDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDS-QTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITM----QDNNSALVDMNKDNPIFIFRALGKILYSKRETDEL-EIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPK-NTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            D L +H+KKI EV +W++     ++    ++ LTGP GSGK+ T+  +    NI I  ++ E   N  EDF            FLL+  R+  L       + K+L+L+   P+Q YS+       L         P+I  +++S      +P  L  K       ID ++F P+A + MKKA+ +        +++ +L    N I E + GD+R A+N L++        + + ++++  +K N    F  +G+ILY+KR    + + +++ ++ ++ + + +   R +L  +P  +     +     +++L +N   F  +I +   + E FS  D+L   W   ++ L   Y+    TRS++++N   P+  T   + +   S ++K   +T+L +  +            ++ +K   + T PY+  I
Sbjct:   19 DELQIHNKKIAEVDHWLKNVFSESNKQLGVMYLTGPAGSGKSTTVEVMCTEQNIEIIEYSPEYLHN--EDFECEKPDFTQLRRFLLR--RHGSLR---GGGLKKRLLLVTELPDQAYSDAEKFREDLSEVLQHIWHPVIFCLTNSIACWNLNPDRLFTKDFNIMNGIDTVTFNPVADSFMKKALVRASNCLSSPLSDAKL----NVIGEEAGGDLRIAMNMLQMNSIGPNADRRSGNSVICASKANREEAFHMIGRILYAKRVNPNVPKPSRFSKRRRKSAPIPEPLVRTELEHDPTDIITMSSMTSEKLLDFLFQNEPIFCSNISKYRYVAETFSMCDFLTGDWTTRKS-LPEDYVAQMATRSVMWNNYKEPRPGTLFAVGRPLRSSLEKHTARTKLELQRLP-----------MIGAKDYAALTCPYITII 428
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Fly
Match: Rad17 (gene:FBgn0025808 transcript:FBtr0082974)

HSP 1 Score: 124.405 bits (311), Expect = 6.873e-30
Identity = 119/462 (25.76%), Postives = 207/462 (44.81%), Query Frame = 3 
Query:  168 NDYLAVHSKKIQEVLNWIQ--KQPXXXXXXXXXXXTGPPGSGKTATLYAL-------------PNIC--IHCWNDETQS-----NIVEDFTDFLLKASRYSELNDEFNNNVGKKLILIKNFPNQFYSEPS-SLHHLLVRYKASGKCPLIIIISDSQTLKYSPHW-LIPKPLQEELKIDNISFAPIAVTLMKKAISKI--IRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEI--------FITMQDN------------NSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDI--QKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKIL 1409
            +D LAVH KK+ E+ +W++  +       + + LLTGP G+GKT TL  L             P  C  ++   D+T       + +E F  FLL+ASRY  L D  N    K+L+L+++FPN   S+   +   LL  Y A GK PL+ I++D+++   +  + L P  L+ + +I++ISF  IA T+M+K++     + Q+     +     + + I+  + GDIR+A+ NL +          T Q N             S L  + +D  I +  ALG++L  K   D+                        +L +PE + +        FVN+++ N+L   ++ID +     D   SD + N +  +   + G   ++   R ++  N   P + W P+R     +I  Q T+ + RL            +G+     +++L +     LVK++
Sbjct:   98 SDDLAVHPKKVGELRDWLRHCEAVRKKFPAQMCLLTGPTGAGKTTTLRVLAKEFGYQLQEWINPIDCEVVNTLGDQTTGASYVGSHLEAFKSFLLRASRYKSLLDSQN----KRLLLVEDFPNVLLSDKEVNFEELLEEYTAYGKSPLVFIVADAKSRGLNISYRLFPDQLKAKHRIEHISFNAIASTIMQKSMKTFCSVMQQNKATYKVPSTAVVDSIVVGAQGDIRNALINLHLSSLKGVSSMPTKQLNVSVSAKGRKKKMQSTLKSIGRDESITLMHALGRVLNPKFNEDK-----------------------TMLHSPEEITEAFNTEPRNFVNFVYANYLPHFKEIDDVVTAINDLGLSDCMLNEYRDDNLSVMG---LNVAIRGVMMSNTC-PVSGWMPVRGPKRINIQPQATLAEQRL------------VGVGYAGIARTLYATEYSSLVKLI 516
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Zebrafish
Match: rad17 (RAD17 checkpoint clamp loader component [Source:ZFIN;Acc:ZDB-GENE-040718-409])

HSP 1 Score: 175.252 bits (443), Expect = 1.180e-46
Identity = 153/486 (31.48%), Postives = 225/486 (46.30%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQ-PXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCWNDETQSN--IVED-----------------------FTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEK---ELQTITNQIIESSNGDIRSAINNLEI-------------------------------------FITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPK------NTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV +W++     +     ILLLTGP G GKTAT+  L       +  W++ + ++    ED                       F +FLL+A++YS L          +K+ILI+ FPNQFY +P  LH +L ++   G+CPL++I+SDS +   +   L    LQ EL I NISF P+A T M K +S+I+  E    + +     +   + +   S+GDIRSAIN+L+                                         MQD + A+    KD  +F+FRALGKILY KRE+ E   A       +L     +  R +LL +PE V +   ++   F  YLH+N+ DF  D++ +++  E  S +D+L   W      L  +Y  S  TR +++ N SR +        + PL K     I KT    R        LF SF      L   SLL+E +PYL K+
Sbjct:   92 LAVHKKKVEEVESWLRVHLDKSKKGGAILLLTGPSGCGKTATVQVLAKELGFQVQEWSNPSTTSQYKTEDLFTQSFDPDSRFNRFHGSSQTGLFQEFLLRANKYSRLRMSGETWTEDRKIILIEEFPNQFYRQPGCLHDILRQFIKCGRCPLVLIVSDSLSGDKNSRLLFKDVLQ-ELGIHNISFNPVAPTSMMKVLSRIVTIEAVKSSGRISVPDKAALDLLCSGSSGDIRSAINSLQFSSFTDNSLERRLWASKKGKSSSAKPAVKAKGRSKSSKSKDMQDESPAI--GGKDASLFLFRALGKILYCKRESYEGTQAP------KLPPHLTDHQRDRLLVDPELVIERSHMSGEFFNLYLHQNYPDFFSDVEDIAQASEYLSDADFLTAEWSSRSTML--EYGSSVATRGLMHSNSSRAQANSQSTAGFKPLHKPHWLHINKTY---RENYQTAQSLFLSF-----CLTPVSLLTEMVPYLAKL 558
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Zebrafish
Match: rad17 (RAD17 checkpoint clamp loader component [Source:ZFIN;Acc:ZDB-GENE-040718-409])

HSP 1 Score: 175.252 bits (443), Expect = 1.180e-46
Identity = 153/486 (31.48%), Postives = 225/486 (46.30%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQ-PXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCWNDETQSN--IVED-----------------------FTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEK---ELQTITNQIIESSNGDIRSAINNLEI-------------------------------------FITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPK------NTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV +W++     +     ILLLTGP G GKTAT+  L       +  W++ + ++    ED                       F +FLL+A++YS L          +K+ILI+ FPNQFY +P  LH +L ++   G+CPL++I+SDS +   +   L    LQ EL I NISF P+A T M K +S+I+  E    + +     +   + +   S+GDIRSAIN+L+                                         MQD + A+    KD  +F+FRALGKILY KRE+ E   A       +L     +  R +LL +PE V +   ++   F  YLH+N+ DF  D++ +++  E  S +D+L   W      L  +Y  S  TR +++ N SR +        + PL K     I KT    R        LF SF      L   SLL+E +PYL K+
Sbjct:   92 LAVHKKKVEEVESWLRVHLDKSKKGGAILLLTGPSGCGKTATVQVLAKELGFQVQEWSNPSTTSQYKTEDLFTQSFDPDSRFNRFHGSSQTGLFQEFLLRANKYSRLRMSGETWTEDRKIILIEEFPNQFYRQPGCLHDILRQFIKCGRCPLVLIVSDSLSGDKNSRLLFKDVLQ-ELGIHNISFNPVAPTSMMKVLSRIVTIEAVKSSGRISVPDKAALDLLCSGSSGDIRSAINSLQFSSFTDNSLERRLWASKKGKSSSAKPAVKAKGRSKSSKSKDMQDESPAI--GGKDASLFLFRALGKILYCKRESYEGTQAP------KLPPHLTDHQRDRLLVDPELVIERSHMSGEFFNLYLHQNYPDFFSDVEDIAQASEYLSDADFLTAEWSSRSTML--EYGSSVATRGLMHSNSSRAQANSQSTAGFKPLHKPHWLHINKTY---RENYQTAQSLFLSF-----CLTPVSLLTEMVPYLAKL 558
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Zebrafish
Match: rad17 (RAD17 checkpoint clamp loader component [Source:ZFIN;Acc:ZDB-GENE-040718-409])

HSP 1 Score: 175.252 bits (443), Expect = 1.312e-46
Identity = 153/486 (31.48%), Postives = 225/486 (46.30%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQ-PXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCWNDETQSN--IVED-----------------------FTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEK---ELQTITNQIIESSNGDIRSAINNLEI-------------------------------------FITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPK------NTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV +W++     +     ILLLTGP G GKTAT+  L       +  W++ + ++    ED                       F +FLL+A++YS L          +K+ILI+ FPNQFY +P  LH +L ++   G+CPL++I+SDS +   +   L    LQ EL I NISF P+A T M K +S+I+  E    + +     +   + +   S+GDIRSAIN+L+                                         MQD + A+    KD  +F+FRALGKILY KRE+ E   A       +L     +  R +LL +PE V +   ++   F  YLH+N+ DF  D++ +++  E  S +D+L   W      L  +Y  S  TR +++ N SR +        + PL K     I KT    R        LF SF      L   SLL+E +PYL K+
Sbjct:   91 LAVHKKKVEEVESWLRVHLDKSKKGGAILLLTGPSGCGKTATVQVLAKELGFQVQEWSNPSTTSQYKTEDLFTQSFDPDSRFNRFHGSSQTGLFQEFLLRANKYSRLRMSGETWTEDRKIILIEEFPNQFYRQPGCLHDILRQFIKCGRCPLVLIVSDSLSGDKNSRLLFKDVLQ-ELGIHNISFNPVAPTSMMKVLSRIVTIEAVKSSGRISVPDKAALDLLCSGSSGDIRSAINSLQFSSFTDNSLERRLWASKKGKSSSAKPAVKAKGRSKSSKSKDMQDESPAI--GGKDASLFLFRALGKILYCKRESYEGTQAP------KLPPHLTDHQRDRLLVDPELVIERSHMSGEFFNLYLHQNYPDFFSDVEDIAQASEYLSDADFLTAEWSSRSTML--EYGSSVATRGLMHSNSSRAQANSQSTAGFKPLHKPHWLHINKTY---RENYQTAQSLFLSF-----CLTPVSLLTEMVPYLAKL 557
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Xenopus
Match: bnip3l (BCL2/adenovirus E1B 19kDa interacting protein 3-like [Source:Xenbase;Acc:XB-GENE-949760])

HSP 1 Score: 183.726 bits (465), Expect = 1.162e-49
Identity = 136/394 (34.52%), Postives = 203/394 (51.52%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQ---KQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCWND-------------ETQSNIVEDFTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEI---------------------FITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR 1226
            LAVH KKI+EV  W++   ++       +ILLLTGPPG GKTAT+  L     I +  W +             ++Q+++   F DFLL+A++Y++L     + +  +K+I++ + PNQFY +PSSLH +L R+  +G+CPL+ IISDS + +     L PK +QEEL ++NISF P+A T M K +++I   E      K +   +   + I   S+GDIRSAIN+L+                          ++ +       KD  +F+FRALGKILY KRE    +   Y+     LS+ N    R  LL  PEAV +   +N   F  YLH+N+L+F  +ID +    E  S +D L   W          Y  S  +R +I+ N +R
Sbjct:   96 LAVHKKKIEEVEAWLKTHVEKRTQKQGGHILLLTGPPGCGKTATIQVLTKEMGIQVQEWINPLMWANTHNSPHFQSQTSL---FHDFLLRANKYNKLQMVGESLSTDRKIIVVDDMPNQFYRDPSSLHDILRRFVKTGRCPLVFIISDSLSGESHQRRLFPKEIQEELVVNNISFNPVAPTTMTKVLTRIASTEANMSGGKIIIPDKASLDLICTGSSGDIRSAINSLQFSAQKATKSGKTSSKSKSKKGSQKGGENIDEVQAIGGKDASLFLFRALGKILYCKREPLPEDTDIYRLP-AHLSEYN----RDLLLVLPEAVVEKSHMNGDLFNLYLHQNYLEFFMEIDDVVRASEYLSSADVLSGDW--NSRSTLSVYSSSVASRGIIHSNSAR 479
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Xenopus
Match: bnip3l (BCL2/adenovirus E1B 19kDa interacting protein 3-like [Source:Xenbase;Acc:XB-GENE-949760])

HSP 1 Score: 181.415 bits (459), Expect = 7.874e-49
Identity = 138/395 (34.94%), Postives = 198/395 (50.13%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQ---KQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCW-NDETQSNIVED--------------------FTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEI-----------------FITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR 1226
            LAVH KKI+EV  W++   ++       +ILLLTGPPG GKTAT+  L     I +  W N   Q    ED                    F DFLL+A++Y++L     + +  +K+I++ + PNQFY +PSSLH +L R+  +G+CPL+ IISDS + +     L PK +QEEL ++NISF P+A T M K +++I   E         +   + I   S+GDIRSAIN+L+                      ++ +       KD  +F+FRALGKILY KRE    +   Y+     LS+ N    R  LL  PEAV +   +N   F  YLH+N+L+F  +ID +    E  S +D L   W          Y  S  +R +I+ N +R
Sbjct:   98 LAVHKKKIEEVEAWLKTHVEKRTQKQGGHILLLTGPPGCGKTATIQVLTKEMGIQVQEWINPLMQEYRQEDRPEAKQGFVLVPYNSSQTSLFHDFLLRANKYNKLQMVGESLSTDRKIIVVDDMPNQFYRDPSSLHDILRRFVKTGRCPLVFIISDSLSGESHQRRLFPKEIQEELVVNNISFNPVAPTTMTKVLTRIASTESGGKIIIPDKASLDLICTGSSGDIRSAINSLQFSAQKGKTSSKSKSKKGSQKGGENIDEVQAIGGKDASLFLFRALGKILYCKREPLPEDTDIYRLPA-HLSEYN----RDLLLVLPEAVVEKSHMNGDLFNLYLHQNYLEFFMEIDDVVRASEYLSSADVLSGDW--NSRSTLSVYSSSVASRGIIHSNSAR 485
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Xenopus
Match: bnip3l (BCL2/adenovirus E1B 19kDa interacting protein 3-like [Source:Xenbase;Acc:XB-GENE-949760])

HSP 1 Score: 179.874 bits (455), Expect = 3.447e-48
Identity = 139/408 (34.07%), Postives = 201/408 (49.26%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQ---KQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCW-NDETQSNIVED----------------------FTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFI-------------------------TMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR 1226
            LAVH KKI+EV  W++   ++       +ILLLTGPPG GKTAT+  L     I +  W N   Q    ED                      F DFLL+A++Y++L     + +  +K+I++ + PNQFY +PSSLH +L R+  +G+CPL+ IISDS + +     L PK +QEEL ++NISF P+A T M K +++I   E      K +   +   + I   S+GDIRSAIN+L+  +                           ++ +       KD  +F+FRALGKILY KRE    +   Y+     LS+ N    R  LL  PEAV +   +N   F  YLH+N+L+F  +ID +    E  S +D L   W          Y  S  +R +I+ N +R
Sbjct:   98 LAVHKKKIEEVEAWLKTHVEKRTQKQGGHILLLTGPPGCGKTATIQVLTKEMGIQVQEWINPLMQEYRQEDRPEVFDRDMGFQPFTSQSQTSLFHDFLLRANKYNKLQMVGESLSTDRKIIVVDDMPNQFYRDPSSLHDILRRFVKTGRCPLVFIISDSLSGESHQRRLFPKEIQEELVVNNISFNPVAPTTMTKVLTRIASTEANMSGGKIIIPDKASLDLICTGSSGDIRSAINSLQFSLLHKKPRFLWPRKTSSKSKSKKGSQKGGENIDEVQAIGGKDASLFLFRALGKILYCKREPLPEDTDIYRLPA-HLSEYN----RDLLLVLPEAVVEKSHMNGDLFNLYLHQNYLEFFMEIDDVVRASEYLSSADVLSGDW--NSRSTLSVYSSSVASRGIIHSNSAR 498
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Xenopus
Match: bnip3l (BCL2/adenovirus E1B 19kDa interacting protein 3-like [Source:Xenbase;Acc:XB-GENE-949760])

HSP 1 Score: 176.792 bits (447), Expect = 5.707e-47
Identity = 141/419 (33.65%), Postives = 203/419 (48.45%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQ---KQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCW-NDETQSNIVED----------------------FTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEI------FIT------------------------------MQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR 1226
            LAVH KKI+EV  W++   ++       +ILLLTGPPG GKTAT+  L     I +  W N   Q    ED                      F DFLL+A++Y++L     + +  +K+I++ + PNQFY +PSSLH +L R+  +G+CPL+ IISDS + +     L PK +QEEL ++NISF P+A T M K +++I   E      K +   +   + I   S+GDIRSAIN+L+       F+T                               ++ +       KD  +F+FRALGKILY KRE    +   Y+     LS+ N    R  LL  PEAV +   +N   F  YLH+N+L+F  +ID +    E  S +D L   W          Y  S  +R +I+ N +R
Sbjct:   99 LAVHKKKIEEVEAWLKTHVEKRTQKQGGHILLLTGPPGCGKTATIQVLTKEMGIQVQEWINPLMQEYRQEDRPEVFDRDMGFQPFTSQSQTSLFHDFLLRANKYNKLQMVGESLSTDRKIIVVDDMPNQFYRDPSSLHDILRRFVKTGRCPLVFIISDSLSGESHQRRLFPKEIQEELVVNNISFNPVAPTTMTKVLTRIASTEANMSGGKIIIPDKASLDLICTGSSGDIRSAINSLQFSAQKGSFLTNNTCSKSRGKATKSGKTSSKSKSKKGSQKGGENIDEVQAIGGKDASLFLFRALGKILYCKREPLPEDTDIYRLPA-HLSEYN----RDLLLVLPEAVVEKSHMNGDLFNLYLHQNYLEFFMEIDDVVRASEYLSSADVLSGDW--NSRSTLSVYSSSVASRGIIHSNSAR 510
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Xenopus
Match: bnip3l (BCL2/adenovirus E1B 19kDa interacting protein 3-like [Source:Xenbase;Acc:XB-GENE-949760])

HSP 1 Score: 176.407 bits (446), Expect = 6.681e-47
Identity = 141/419 (33.65%), Postives = 203/419 (48.45%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQ---KQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCW-NDETQSNIVED----------------------FTDFLLKASRYSELNDEFNN-NVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEI------FIT------------------------------MQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR 1226
            LAVH KKI+EV  W++   ++       +ILLLTGPPG GKTAT+  L     I +  W N   Q    ED                      F DFLL+A++Y++L     + +  +K+I++ + PNQFY +PSSLH +L R+  +G+CPL+ IISDS + +     L PK +QEEL ++NISF P+A T M K +++I   E      K +   +   + I   S+GDIRSAIN+L+       F+T                               ++ +       KD  +F+FRALGKILY KRE    +   Y+     LS+ N    R  LL  PEAV +   +N   F  YLH+N+L+F  +ID +    E  S +D L   W          Y  S  +R +I+ N +R
Sbjct:   85 LAVHKKKIEEVEAWLKTHVEKRTQKQGGHILLLTGPPGCGKTATIQVLTKEMGIQVQEWINPLMQEYRQEDRPEVFDRDMGFQPFTSQSQTSLFHDFLLRANKYNKLQMVGESLSTDRKIIVVDDMPNQFYRDPSSLHDILRRFVKTGRCPLVFIISDSLSGESHQRRLFPKEIQEELVVNNISFNPVAPTTMTKVLTRIASTEANMSGGKIIIPDKASLDLICTGSSGDIRSAINSLQFSAQKGSFLTNNTCSKSRGKATKSGKTSSKSKSKKGSQKGGENIDEVQAIGGKDASLFLFRALGKILYCKREPLPEDTDIYRLP-AHLSEYN----RDLLLVLPEAVVEKSHMNGDLFNLYLHQNYLEFFMEIDDVVRASEYLSSADVLSGDW--NSRSTLSVYSSSVASRGIIHSNSAR 496
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Mouse
Match: Rad17 (RAD17 checkpoint clamp loader component [Source:MGI Symbol;Acc:MGI:1333807])

HSP 1 Score: 179.489 bits (454), Expect = 4.917e-48
Identity = 147/482 (30.50%), Postives = 222/482 (46.06%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCWNDETQSNIVED-----------------------FTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDN--------------------------NSALVDM------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT T+  L     I +  W +    +  +D                       F DFLL+A++YS+L    D+   +  KK+IL++  PNQFY +P++LH +L ++   G+CPL+ I+SDS +   +   L P+ +QEE  + NISF P+A T+M K +++I+  E     EK +   +T    + +  +GDIRSAIN+L+   +  +N                          NS L +        KD  +F+FRALGKILY KR    EL+  +    L +         R  LL  PE + +   +    F  YLH+N++DF  ++D L    E  S++D L   W      L  +Y  S  TR +++ N +R        +++ PL K     IQK   +  L       LF  F      L +  L ++ LPYL 
Sbjct:  102 LAVHKKKIEEVETWLKAQVLEVKPKQGGSVLLITGPPGCGKTTTIKILSKELGIQVQEWVNPILPDFQKDDYKELLSLESNFSVVPYQSQIAVFNDFLLRATKYSKLQMLGDDLTTD--KKIILVEELPNQFYRDPNALHEILRKHVQIGRCPLVFIVSDSVSGDNNQRLLFPRNIQEECSVSNISFNPVAPTIMMKFLNRIVTIEASKNGEKIIVPNKTSLELLCQGCSGDIRSAINSLQFSSSKGENSSWSKKKRMSLKSDAAISKSKQKKKHNSTLENQEIQAIGGKDVSLFLFRALGKILYCKRAPLTELDSPRLPAHLSEHD-------RDTLLVQPEEIVEMSHMPGDFFNLYLHQNYIDFFAEVDDLVPASEFLSFADILGGDW--NTRSLLREYSTSVATRGVMHSNKARGFAHCQGGSSFRPLHKPQWFLIQKKYRENCLA---AKALFVDF-----CLPALCLQTQLLPYLA 564
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Mouse
Match: Rad17 (RAD17 checkpoint clamp loader component [Source:MGI Symbol;Acc:MGI:1333807])

HSP 1 Score: 179.489 bits (454), Expect = 4.917e-48
Identity = 147/482 (30.50%), Postives = 222/482 (46.06%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCWNDETQSNIVED-----------------------FTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDN--------------------------NSALVDM------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT T+  L     I +  W +    +  +D                       F DFLL+A++YS+L    D+   +  KK+IL++  PNQFY +P++LH +L ++   G+CPL+ I+SDS +   +   L P+ +QEE  + NISF P+A T+M K +++I+  E     EK +   +T    + +  +GDIRSAIN+L+   +  +N                          NS L +        KD  +F+FRALGKILY KR    EL+  +    L +         R  LL  PE + +   +    F  YLH+N++DF  ++D L    E  S++D L   W      L  +Y  S  TR +++ N +R        +++ PL K     IQK   +  L       LF  F      L +  L ++ LPYL 
Sbjct:  102 LAVHKKKIEEVETWLKAQVLEVKPKQGGSVLLITGPPGCGKTTTIKILSKELGIQVQEWVNPILPDFQKDDYKELLSLESNFSVVPYQSQIAVFNDFLLRATKYSKLQMLGDDLTTD--KKIILVEELPNQFYRDPNALHEILRKHVQIGRCPLVFIVSDSVSGDNNQRLLFPRNIQEECSVSNISFNPVAPTIMMKFLNRIVTIEASKNGEKIIVPNKTSLELLCQGCSGDIRSAINSLQFSSSKGENSSWSKKKRMSLKSDAAISKSKQKKKHNSTLENQEIQAIGGKDVSLFLFRALGKILYCKRAPLTELDSPRLPAHLSEHD-------RDTLLVQPEEIVEMSHMPGDFFNLYLHQNYIDFFAEVDDLVPASEFLSFADILGGDW--NTRSLLREYSTSVATRGVMHSNKARGFAHCQGGSSFRPLHKPQWFLIQKKYRENCLA---AKALFVDF-----CLPALCLQTQLLPYLA 564
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. UniProt/SwissProt
Match: sp|Q6NXW6|RAD17_MOUSE (Cell cycle checkpoint protein RAD17 OS=Mus musculus OX=10090 GN=Rad17 PE=1 SV=2)

HSP 1 Score: 179.489 bits (454), Expect = 3.439e-47
Identity = 147/482 (30.50%), Postives = 222/482 (46.06%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCWNDETQSNIVED-----------------------FTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDN--------------------------NSALVDM------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT T+  L     I +  W +    +  +D                       F DFLL+A++YS+L    D+   +  KK+IL++  PNQFY +P++LH +L ++   G+CPL+ I+SDS +   +   L P+ +QEE  + NISF P+A T+M K +++I+  E     EK +   +T    + +  +GDIRSAIN+L+   +  +N                          NS L +        KD  +F+FRALGKILY KR    EL+  +    L +         R  LL  PE + +   +    F  YLH+N++DF  ++D L    E  S++D L   W      L  +Y  S  TR +++ N +R        +++ PL K     IQK   +  L       LF  F      L +  L ++ LPYL 
Sbjct:  102 LAVHKKKIEEVETWLKAQVLEVKPKQGGSVLLITGPPGCGKTTTIKILSKELGIQVQEWVNPILPDFQKDDYKELLSLESNFSVVPYQSQIAVFNDFLLRATKYSKLQMLGDDLTTD--KKIILVEELPNQFYRDPNALHEILRKHVQIGRCPLVFIVSDSVSGDNNQRLLFPRNIQEECSVSNISFNPVAPTIMMKFLNRIVTIEASKNGEKIIVPNKTSLELLCQGCSGDIRSAINSLQFSSSKGENSSWSKKKRMSLKSDAAISKSKQKKKHNSTLENQEIQAIGGKDVSLFLFRALGKILYCKRAPLTELDSPRLPAHLSEHD-------RDTLLVQPEEIVEMSHMPGDFFNLYLHQNYIDFFAEVDDLVPASEFLSFADILGGDW--NTRSLLREYSTSVATRGVMHSNKARGFAHCQGGSSFRPLHKPQWFLIQKKYRENCLA---AKALFVDF-----CLPALCLQTQLLPYLA 564
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. UniProt/SwissProt
Match: sp|Q9XT62|RAD17_CHLAE (Cell cycle checkpoint protein RAD17 OS=Chlorocebus aethiops OX=9534 GN=RAD17 PE=1 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 1.560e-46
Identity = 153/484 (31.61%), Postives = 222/484 (45.87%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT T+  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI +ISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  DID +    E  S++D L   W      L  +Y  S  TR +I+ N +R         +++ PL K     I K   +  L       LFP F      L +  L ++ LPYL 
Sbjct:   92 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTIKVLSKEHGIQVQEWINPVLPDFQKDDFREIFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFVISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLWPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMDIDDIVRASEFLSFADILSGDW--NTCSLLREYSTSIATRGVIHSNKARGYAHCQGGGSSFRPLHKPQWFLIYKKYRENCLA---AKALFPDF-----CLPALCLQTQLLPYLA 555
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. UniProt/SwissProt
Match: sp|O75943|RAD17_HUMAN (Cell cycle checkpoint protein RAD17 OS=Homo sapiens OX=9606 GN=RAD17 PE=1 SV=2)

HSP 1 Score: 177.178 bits (448), Expect = 2.381e-46
Identity = 153/484 (31.61%), Postives = 222/484 (45.87%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT TL  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI IISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  +ID +    E  S++D L   W      L  +Y  S  TR +++ N +R         +++ PL K     I K   +  L       LFP F      L +  L ++ LPYL 
Sbjct:  103 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDW--NTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLA---AKALFPDF-----CLPALCLQTQLLPYLA 566
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. UniProt/SwissProt
Match: sp|Q5R652|RAD17_PONAB (Cell cycle checkpoint protein RAD17 OS=Pongo abelii OX=9601 GN=RAD17 PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 4.664e-46
Identity = 152/484 (31.40%), Postives = 222/484 (45.87%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXX---TGPPGSGKTATLYALP---NICIHCW-----------------NDET-------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN------------SALVDM--------------------NKDNPIFIFRALGKILYSKRET-DELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISR-------PKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KKI+EV  W++ Q L         +   TGPPG GKT T+  L     I +  W                 N E+       QS I   F +FLL+A++Y++L    D+   +  KK+IL+++ PNQFY +  +LH +L +Y   G+CPLI IISDS +   +   L PK +QEE  I NISF P+A T+M K +++I+  E      K     +T    + +  +GDIRSAIN+L+   +  +NN             A++                       KD  +F+FRALGKILY KR +  EL+  +    L +         R  LL  PE V +   +    F  YLH+N++DF  +ID +    E  S++D L   W      L  +Y  S  TR +++ N +R         +++ PL K     I K   +  L       LFP F      L +  L ++ LPYL 
Sbjct:  103 LAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTIKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAV-FKEFLLRATKYNKLQMLGDDLRTD--KKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLWPRKKGMSLKSDAVLSKSKQRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYE-------RDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDW--NTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENYLA---AKALFPDF-----CLPALCLQTQLLPYLA 566
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. UniProt/SwissProt
Match: sp|Q9MBA3|RAD17_ARATH (Cell cycle checkpoint protein RAD17 OS=Arabidopsis thaliana OX=3702 GN=RAD17 PE=1 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 4.655e-20
Identity = 111/451 (24.61%), Postives = 191/451 (42.35%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQP---XXXXXXXXXXXTGPPGSGKTATLYALPNI---CIHCWN-----------DETQSNI-----VEDFTDFLLKASRYSELNDEFNNNVGKKLIL-IKNFPNQFYSEP-SSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQ------EELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEIFIT---------------MQDN------------NSALVD-MNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFR--PQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDY----------LFNRWLPEQN--DLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDIQKTILQTRLGIDE 1313
            L+VH KK+ EV  W Q+      N   +N+LL+TG  G GK+AT++ + +I    +H WN             T S +     +++F +F+    +Y  +     + +   ++L I + P          L + L+    S + P +I+I+D      S      + ++      E      ++F PI    +KK + +I R+E   +   E+    +Q+  +S GDIR AI +L++F                 M+DN            +S +     +D  + +F ALGK L++KRETD + I++        S L  +EF   P  +  PE V           V++LHEN LDF+ D      I + +  S Y          +  +     N  D+      S   R ++Y N     + WH +RK  L  ++++ +QT+  + E
Sbjct:  105 LSVHKKKVDEVKLWFQESLDFLKNGLRNNVLLVTGQAGVGKSATIHLITSILGVTVHEWNAPIPTIWQEHVHNTSSGLKYTSKLDEFENFVESTRKYGVMASSSTDGIKAPVVLLIDDLPLANGRHAFERLQNCLLLLVKSTQIPTVILITDHNNADSSDQ--TARRMEDTQSSLERAGALKVAFNPITKNSIKKVLQRICREEHCKVTTMEI----DQMATASGGDIRHAITSLQLFSVKPELNHTKIKSPRPGMEDNYHGNEQTMYSGLDSGISSCFGRDETLSLFHALGKFLHNKRETDNVIISECS------SSLVHDEFARLPLKMDAPEKVLSQAHGQAGRVVDFLHENVLDFVSD----GAIEDAWCVSSYLADADLLLADIRGKMSGHNNTEDVPQSAGASVAVRGVLYGNKQPCSSRWHVIRKPKLWQVEQSSIQTKKNLRE 539

HSP 2 Score: 27.335 bits (59), Expect = 4.655e-20
Identity = 9/25 (36.00%), Postives = 20/25 (80.00%), Query Frame = 1 
Query:  124 KNKSSVLWIEKYKPKTIT*LFIVKR 198
            ++K++ LW++KY+P+T+  L + K+
Sbjct:   86 RSKNTDLWVDKYRPRTLEELSVHKK 110
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. TrEMBL
Match: A0A2D0PLZ5 (cell cycle checkpoint protein RAD17 isoform X4 OS=Ictalurus punctatus OX=7998 GN=rad17 PE=4 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 1.729e-52
Identity = 156/488 (31.97%), Postives = 232/488 (47.54%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQ---PXXXXXXXXXXXTGPPGSGKTATLYALP-NICIHC--WNDETQSNIVED------------------------FTDFLLKASRYSELNDEFNNNVG-KKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQE------RFYMNEKELQTITNQIIESSNGDIRSAINNLEIFI--------------------------TMQDNNSALVDM--------NKDNPIFIFRALGKILYSKRETDEL-EIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNT------WHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV +W++       +     +LLLTGP G GKTAT++ L  + C H   W + T +    D                        F +FLL+A++Y+ L    ++    +K+IL+++FPNQFY +P  LH +L R+  +G+CPL+ I+SDS +   S   L PK +QEEL I NISF P+A T M K +S+I   E      R  + +K       Q+   S+GDIRSA+N+L+                               +  ++   D+         KD  +F+FRALGKILY KRE+ E  ++ K    L +         R +LL +PE V +   ++   F  YLH+N+LDF  D++ ++   E  S +D+L   W      L  +Y  S  TR +IY N +R +        + PL K     I KT  +  L       LF SF      L   SL++E +PYL K+
Sbjct:  124 LAVHKKKVEEVESWLKVHLDLKSSKKGGALLLLTGPSGCGKTATVHVLSKDACCHIQEWTNPTTAEYRTDDSFRESFNPASRFNIFHSSSQTAAFQEFLLRANKYNRLQMSGDDEAQERKIILVEDFPNQFYRQPGCLHDILRRFVRTGRCPLVFIVSDSLSGDTSSRLLFPKGVQEELSIHNISFNPVAPTSMMKVLSRIATAEASKSAGRISVPDK---AALEQLCSGSSGDIRSAVNSLQFSCFTDFSLEKSLWASKKGKSASKPSARPKGRGRSAKSTDVPEESPAIGGKDASLFLFRALGKILYCKRESCETGDVPKLPAHLSE-------HQRDKLLVDPELVIERSHMSGEFFNLYLHQNYLDFFSDVEDVARASEYLSDADFLTAEWTSRSTML--EYGSSVATRGLIYSNSARARAAHQSSVGFKPLHKPHWLHINKTYRENYL---TAQSLFVSF-----CLTPISLVTELVPYLAKL 591
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. TrEMBL
Match: A0A3B4D1X8 (RAD17 checkpoint clamp loader component OS=Pygocentrus nattereri OX=42514 PE=4 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 2.132e-51
Identity = 157/488 (32.17%), Postives = 240/488 (49.18%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQ---PXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCWNDETQSNIVED------------------------FTDFLLKASRYSELNDEFNNNV-GKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEK---ELQTITNQIIESSNGDIRSAINNLE------------IFITMQDNNSA--------------------LVD-----MNKDNPIFIFRALGKILYSKRETDEL-EIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNT------WHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV +W++      + +    ILLLTGP GSGKTAT++ L    +  I  W++ T S    D                        F +FLL+A++Y+ L    ++    +++IL+++FPNQFY +P SLH +L R+  +GKCP+I I+SDS +   S   L PK +Q+EL I +ISF P+A T M K +S+I   E      K     +   +Q+   S+GDIRSAIN+L+            ++ + +  ++A                    ++D       KD  +F+FRALGKILY KRE+ E+ E+ K    L +         R +LL +PE V +   ++   F  YLH+N+LDF  ++D ++   E  S +D+L   W      L  +Y  S  TR +I+ N SR +        + PL K     I +T  +  L       LF     +N  L   SL++E +PYL K+
Sbjct:  103 LAVHKKKVEEVESWLRVHLDLKIANKGGAILLLTGPSGSGKTATVHVLAKDVDFQIQEWSNPTTSEYRMDDSFRQSFDPDSRFNSFHGSSQTGAFQEFLLRANKYNRLQMSGDSQAQSRRIILVEDFPNQFYRQPGSLHDILRRFVKTGKCPVIFIVSDSLSGDTSSRLLFPKEIQQELGIHHISFNPVAPTSMLKVLSRIAAVEASKSAGKISVPDKAALDQLCSGSSGDIRSAINSLQFSSFTDFSLEKGLWASKKGKSAASSFKAPVRAKGRSRSVKSTDVLDDSPAIGGKDASLFLFRALGKILYCKRESYEVSEVPKLPAHLAE-------HQRDRLLVDPELVIERSHMSGEFFNLYLHQNYLDFYLNVDDVARASEYLSDADFLTAEWTSRSTML--EYGSSVATRGLIHSNSSRAQAACQSSVGFKPLHKPHWLQINRTYRENYL---SAQSLF-----INFCLAPISLVTELVPYLAKL 573
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. TrEMBL
Match: A0A3B4U1P3 (RAD17 checkpoint clamp loader component OS=Seriola dumerili OX=41447 PE=4 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 5.483e-51
Identity = 165/475 (34.74%), Postives = 225/475 (47.37%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXXTGPPGSGKTATLYALPNICIHCW---------------------------NDETQSNIVEDFTDFLLKASRYSELNDEFNNNVG-KKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQDNN--SALVDMNKDNPI-----------------------------FIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDF---IEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KKI+EV NW++    NS+   ILLLTGP G GKTAT+  L      CW                           N  + S+ V  F +FLL+A++Y+ L    +     KKLIL+++ PNQFY +P SLH  L R+  +G+CPL+ I+SDS +   S  +L P+ +QEEL I +ISF P+A T M K +S+I   E      +     Q +   +   S+GDIRSAIN+L+ F ++ D +    L  M KD P+                             F+FRALGKIL+ KR T E   A        LS    +  R  LL +PE V D   ++   F  YLH+N+LDF   +ED+D+ SE   D   +D L   W        G Y  S  TR +++ N  +    + PL K     I K   +  L       LF SF      L   SL +E LPYL K+
Sbjct:   94 LAVHKKKIEEVENWMRVH-TNSSKGGILLLTGPSGCGKTATVQVL------CWELGLRIQEWINPTNVEPYSSSQHEWRMNGLSCSSQVAQFQEFLLRANKYNCLRMAGDGGAADKKLILVEDLPNQFYRQPGSLHDNLRRFVKTGRCPLVFIVSDSLSGDSSSRFLFPREIQEELDICSISFNPVAPTTMMKVLSRISALEAGKSCGRICVPDQAVLETLCSGSSGDIRSAINSLQ-FSSLPDTSLEKRLWTMKKDRPVTSRTNRRKRSKMTKEHEEEKAIGGKDASLFLFRALGKILHCKRGTHEGGEAAEGASGPGLSSHLSHHHREALLVDPELVVDRSHMSGEFFNLYLHQNYLDFFSEVEDVDRASEYLSD---ADLLTADWTSRST--MGDYGCSVATRGLLHSNSQQVSVGFRPLHKPSWLLISKKHRENCLA---AQSLFRSF-----CLAPVSLQTELLPYLAKL 547
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. TrEMBL
Match: A0A3Q4AZM9 (RAD17 checkpoint clamp loader component OS=Mola mola OX=94237 PE=4 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 1.700e-50
Identity = 160/478 (33.47%), Postives = 233/478 (48.74%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCWNDET------QSNIVED-------------------FTDFLLKASRYSELNDEFNNNVG-KKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQE------RFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQDNN--SALVDMNKDNP----------------------------IFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDF---IEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV NW++     ++   ILLLTGP G GKTAT+  L     + I  W + T      +S   E+                   F DFLL+A++Y+ L    +     KKLIL+++FPNQFY +P +LH +L  +  + +CPL+ I+SDS +   S  +L P+ +QEEL I NISF P+A T M K +++I   E      R  +++   QT+   +   S+GDIRSAIN+L+ F ++ D +    L  M KD P                            +F+FRALGKIL+ KR+ +  E A        LS    + +R  LL NPE V +   ++   F+ YLH+N+LDF   +ED+D  SE   D   +D L   W+       G Y  S  TR +++ N  +    + PL K     + K   +  L       LF SF      +   SL +E LPYL K+
Sbjct:   99 LAVHKKKMEEVENWLRNH--ITSEGGILLLTGPSGCGKTATVQVLAQELGLRIQEWTNPTNLEPYSRSQHGENGTLKEPTPPSYGPVSQFAQFQDFLLRANKYNCLKMVGDGGATEKKLILVEDFPNQFYRQPGNLHDILRHFVKTSRCPLVFIVSDSLSGDGSLRFLFPRDIQEELDISNISFNPVAPTTMLKVLTRISTLEAGKSCGRVPISD---QTVLETLCSGSSGDIRSAINSLQ-FFSLPDMSLEKGLWRMRKDRPASRTSRTKKSKQTKQLEDEHAFGGKDASLFLFRALGKILHCKRDPEAGECASGPGLPPHLS----HHYRNMLLVNPELVVERSHMSGEFFILYLHQNYLDFFSEVEDVDHASEYLSD---ADLLSADWM--NRSTMGDYGSSVATRGLLFSNSQQVSVGFRPLHKPNWLLVNKNHKENCLA---AQSLFRSF-----CMMPVSLQTELLPYLAKL 553
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. TrEMBL
Match: W5KBU7 (RAD17 checkpoint clamp loader component OS=Astyanax mexicanus OX=7994 PE=4 SV=2)

HSP 1 Score: 194.897 bits (494), Expect = 3.877e-50
Identity = 160/494 (32.39%), Postives = 240/494 (48.58%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQ---PXXXXXXXXXXXTGPPGSGKTATLYAL-------------PNICIHCWNDET---------------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQE------RFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQDNN------------------------------SALVDMNKDNP--------IFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNT------WHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV +W++ Q      +    +LLLTGP G GKTAT++ L             PN       D++                S+    F +FLL+A++Y+ L    D  + N  +K IL+++FPNQFY +P  LH +L R+  +GKCPL+ I+SDS +   S   L PK +Q+EL I +ISF P+A T M K +S+I   E      R  + +K   T+ +Q+   S+GDIRSAIN+L+ F +  DN+                              +   D  +D+P        +F+FRALGKILY KRE  E        K+   + L +++ R +LL +PE V +   ++   F  YLH+N+LDF  +++ ++   E  S +D+    W      L  QY  S  TR +I+ N SR + T      + PL K     I KT  +  L       LF     +N  L   +L++E +PYL K+
Sbjct:  103 LAVHKKKVEEVESWLRVQLDLRSENKGGAVLLLTGPSGCGKTATVHVLAKDLGFQIQEWSNPNTASEYRTDDSFRQSFDPASRFNSFHGSSQTGAFQEFLLRANKYNRLQMTGDTQSQN--RKAILVEDFPNQFYRQPGCLHDILRRFVKTGKCPLVFIVSDSLSGDTSSRLLFPKDIQQELGIHHISFNPVAPTSMMKVLSRIASIEASKNAGRISVPDK---TVLDQLCSGSSGDIRSAINSLQ-FSSFTDNSLEKGLWASKKGKSAASAAKAPTRARGRSRATKSTDKLEDSPAIGGKDASLFLFRALGKILYCKREGYEGYEGSEVPKIP--AHLAEHQ-RDRLLVDPEIVIERSHMSGEFFNLYLHQNYLDFFSNVEDVARASEYLSDADFFTAEWSSRSTML--QYGSSVATRGLIHSNSSRAQATSQSSVGFKPLHKPHWLQINKTYRENYL---SAQSLF-----INFCLAPINLVTELVPYLAKL 577
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Cavefish
Match: rad17 (RAD17 checkpoint clamp loader component [Source:NCBI gene;Acc:103031875])

HSP 1 Score: 194.897 bits (494), Expect = 1.469e-53
Identity = 160/494 (32.39%), Postives = 240/494 (48.58%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQ---PXXXXXXXXXXXTGPPGSGKTATLYAL-------------PNICIHCWNDET---------------QSNIVEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQE------RFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQDNN------------------------------SALVDMNKDNP--------IFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNT------WHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK++EV +W++ Q      +    +LLLTGP G GKTAT++ L             PN       D++                S+    F +FLL+A++Y+ L    D  + N  +K IL+++FPNQFY +P  LH +L R+  +GKCPL+ I+SDS +   S   L PK +Q+EL I +ISF P+A T M K +S+I   E      R  + +K   T+ +Q+   S+GDIRSAIN+L+ F +  DN+                              +   D  +D+P        +F+FRALGKILY KRE  E        K+   + L +++ R +LL +PE V +   ++   F  YLH+N+LDF  +++ ++   E  S +D+    W      L  QY  S  TR +I+ N SR + T      + PL K     I KT  +  L       LF     +N  L   +L++E +PYL K+
Sbjct:  103 LAVHKKKVEEVESWLRVQLDLRSENKGGAVLLLTGPSGCGKTATVHVLAKDLGFQIQEWSNPNTASEYRTDDSFRQSFDPASRFNSFHGSSQTGAFQEFLLRANKYNRLQMTGDTQSQN--RKAILVEDFPNQFYRQPGCLHDILRRFVKTGKCPLVFIVSDSLSGDTSSRLLFPKDIQQELGIHHISFNPVAPTSMMKVLSRIASIEASKNAGRISVPDK---TVLDQLCSGSSGDIRSAINSLQ-FSSFTDNSLEKGLWASKKGKSAASAAKAPTRARGRSRATKSTDKLEDSPAIGGKDASLFLFRALGKILYCKREGYEGYEGSEVPKIP--AHLAEHQ-RDRLLVDPEIVIERSHMSGEFFNLYLHQNYLDFFSNVEDVARASEYLSDADFFTAEWSSRSTML--QYGSSVATRGLIHSNSSRAQATSQSSVGFKPLHKPHWLQINKTYRENYL---SAQSLF-----INFCLAPINLVTELVPYLAKL 577
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Sea Lamprey
Match: rad17 (RAD17 checkpoint clamp loader component [Source:ZFIN;Acc:ZDB-GENE-040718-409])

HSP 1 Score: 170.244 bits (430), Expect = 3.451e-47
Identity = 131/424 (30.90%), Postives = 199/424 (46.93%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXX--XXXXXXXXXTGPPGSGKTATLYALP---NICIHCW-----------NDET----------QSNI---VEDFTDFLLKASRYSEL---NDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITMQD--NN--------------------------------SALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWL--PEQNDLFGQYLISTVTRSMIYHNISRPKN 1235
            LAVH KK++EV  W++   L   +   +ILL+TGP G+GKTATL  L     I IH W             ET           S +   V  F +FLL+A+RY  L     E +     +LIL+++ PNQFY EPSSLH +L ++   G+CPL+++ SD    +YS   L+P+ +QE+L +  ISF P++ T + K + ++   E   +  +     QT+   +  SS GDIR AIN L+     +   NN                                S  V   +D  +F+F ALGKIL+ KRE   +      E     + L   + R  L  NPE V +   ++   F  YLH+N+L F+ D + +S   +  S +D L   W    E  D    Y+ +  TR +++ ++ R ++
Sbjct:    5 LAVHKKKVEEVETWLRAHALQYNARKGSILLITGPAGAGKTATLQVLCKQLGIIIHEWINPLEIGGNGFGKETFGWEREPRGFGSGVQTQVALFREFLLRANRYRALCLNGGEASVEPEARLILLEDIPNQFYREPSSLHDVLRKFSICGRCPLVLVASDCSGSEYSLRTLLPRGIQEDLGVHVISFNPVSPTSLLKVLMRVASAETAQVRGRPCVLNQTVLQDLCASSAGDIRGAINALQFAWQCESLLNNDSTSKKWGKLNKTDLPGRRGRRGTESPTSPEESRTVFGGRDVSLFLFHALGKILHCKRENGPI-----SESHALPTHLTHQQ-RNMLAVNPEDVVERSQMSPELFTLYLHQNYLGFLHDAEDVSAAAQHLSDADCLTADWAVRVEMRD----YVAAVATRGLLHSHVGRARS 418
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Yeast
Match: RAD24 (Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein [Source:SGD;Acc:S000000975])

HSP 1 Score: 54.299 bits (129), Expect = 3.460e-8
Identity = 66/288 (22.92%), Postives = 125/288 (43.40%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXXTGPPGSGKTATLYALPNICIHCWNDETQSNI------------------------VEDFTDFLLKASRYSELNDEFNNNVGKKLILIKNFPNQFYSEPSS-LHHLLVRYKASGKC---PLIIIISDSQT-------------LKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIE---SSNGDIRSAINNLEIFITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETD 908
            +A+H +K+++V   +    L +    ILLL+GP G  K+  +  L  I +  +   +                            +E F++FL K +RY  +++         LILI++ PN F+ +       L++++  S +    PL+I I++ +                +S   ++ K +    ++  I F PI  TL+KK +  I  Q    + EK       ++I+      GDIRSAI  L+ + T   ++ +L    +++ I  F A+GK+++    T+
Sbjct:   78 VAIHKRKLKDVQEALDAMFLPNAKHRILLLSGPSGCSKSTVIKELSKILVPKYRQNSNGTSFRSTPNEHKVTEFRGDCIVNDLPQMESFSEFL-KGARYLVMSN-------LSLILIEDLPNVFHIDTRRRFQQLILQWLYSSEPLLPPLVICITECEIPENDNNYRKFGIDYTFSAETIMNKEILMHPRLKRIKFNPINSTLLKKHLKFICVQNMKMLKEKNKWNKRQEVIDYIAQETGDIRSAITTLQFWAT---SSGSLPISTRESTISYFHAIGKVIHGSHSTN 354
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Nematostella
Match: EDO34493 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP76])

HSP 1 Score: 166.392 bits (420), Expect = 5.885e-45
Identity = 150/489 (30.67%), Postives = 227/489 (46.42%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCW------NDET---------QSNI--------VEDFTDFLLKASRYSELN--DEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQE------RFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQDNNSALVDMNK-------------------------------------DNPIFIFRALGKILYSKRETDELEIAKYKEKL-QQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHN--------ISRP-KNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLV 1400
            LAVH KK+ EV  W+ +Q  +  S+ ILLLTGP G+GKTAT++ L    +  I  W       DE          +SN         +  F +FLL+A++Y  L+      +   +K+IL+++ PN F+ +    H +L  Y+++G+ P+++IISDS     + H L+PK +QE L + +ISF PIA T + K +++I   E       F +  KE       + +SS GDIR+AIN L+ F  ++ NN +L+   K                                     D+ IF+FRALGKILY KR+     +   K+ L   L+   +N   PQ  F PE V +   L+      YLH+N+LDF  D+D +    E  S +D +   W+     L   Y+ S  TR +++ N         S P +  W PL K    +I K +  ++        LFP       +L      +E LPYL 
Sbjct:   16 LAVHKKKVAEVRCWLLEQLTSKPSNAILLLTGPVGAGKTATIHMLQTELSFEIQEWINPLVTTDEVDYVKLVQCLRSNFNFMSYQSQLSQFKEFLLRANKYPTLSIFGTSTDAKPRKVILVEDLPNIFFRDSKKFHEVLQSYQSAGRSPVVLIISDSHHGDSNVHRLLPKSVQESLGVTSISFNPIARTSLVKTLNRIASSENQKAGREFVLPGKE---AIEGLAQSSTGDIRTAINALQ-FSCLRGNNLSLLKKTKSGTSVQKTSWQNRLSEDSSRAGQDSGEPSVLSAIGGRDSSIFLFRALGKILYCKRD----PVTSDKDLLPPHLTHCARNT--PQ--FCPEEVAERAHLSSELLQLYLHQNYLDFFTDLDSVVIALEYMSDADMMSAVWM--HRSLLVPYMASIATRGLMFANARYGPTPGFSAPSRGGWRPLHKPQWFEINKRMRDSK---QAARALFPDHASNATVLH-----TEILPYLA 482
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Medaka
Match: rad17 (RAD17 checkpoint clamp loader component [Source:NCBI gene;Acc:101168964])

HSP 1 Score: 172.555 bits (436), Expect = 4.476e-46
Identity = 143/472 (30.30%), Postives = 206/472 (43.64%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXXTGPPGSGKTATLYALP---NICIHCWNDE-------------------TQSNIVEDFTDFLLKASRYSELNDEFNNNV-GKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKEL---QTITNQIIESSNGDIRSAINNLEIFITM-QDNNSALVDMNKDNP-----------------------------------IFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKI 1406
            LAVH KK+++V  W+       +   IL+LTGP GSGKTAT+  L     + +  W +                    T S+ V  F +FLL+A +Y  L+   +    GKKLIL+++FPNQF  +PSSLH LL R+    +CPL+ ++S+S +   S   L P+ +QEEL I+ ISF P+A T M K +  I  QE      +     Q     +   S GDIRSAIN+L+    +    NS +    KD P                                   +F+FRALGKIL+ KR     E+         L     +  R  LL  PE V +   ++   F  YLH+N+LDF  +++ +    E  S +D L   W      +   Y  S   R +++ N  +    + PL K     +     +  L       LF  F      L +  L  E LPYL K+
Sbjct:   80 LAVHKKKVEDVQTWMNAH---RSKGGILVLTGPSGSGKTATVRVLSQELGLKVQEWTNPSNVEPYSSWQNADWRIDGLTYSSQVTQFREFLLRAYKYGCLSMAGDGGAAGKKLILVEDFPNQFSRQPSSLHDLLRRFVRIARCPLVFVVSESVSGDGSVRSLFPREIQEELHINCISFNPVAPTSMMKVLGSISAQEAVKSGGRTSVPGQAELEALCSGSAGDIRSAINSLQFLCLLGSSGNSGVSRTKKDRPASGRRGASRSNQKTKKTKTVKDQEEERAIGGKDAALFLFRALGKILHCKR-GGHTEVPSPALPPHLL-----HHCREDLLVEPEVVVERSHVSAEMFNLYLHQNYLDFFSEVEDVERASEYLSDADLLAADWT--NRIILRDYSSSVAVRGILHSNSHQVSVGFRPLHKPAWLQVSTKHRENCLA---ARHLFRDF-----CLPAACLQVELLPYLAKL 532
[Close]
BLAST of RAD17 checkpoint clamp loader component vs. Planmine SMEST
Match: SMESG000009809.1 (SMESG000009809.1)

HSP 1 Score: 969.918 bits (2506), Expect = 0.000e+0
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 3 
Query:  177 LAVHSKKIQEVLNWIQKQPXXXXXXXXXXXTGPPGSGKTATLYALPNICIHCWNDETQSNIVEDFTDFLLKASRYSELNDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKILPKDYRYSGKWKNFNFLKELEFNKNKSSKYEKLDDKMPVESPDFSQLSFHRKKRDNDEWIVCKNANEDNVSIEIDD 1634
            LAVHSKKIQEVLNWIQKQPLNSNSSNILLLTGPPGSGKTATLYALPNICIHCWNDETQSNIVEDFTDFLLKASRYSELNDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKILPKDYRYSGKWKNFNFLKELEFNKNKSSKYEKLDDKMPVESPDFSQLSFHRKKRDNDEWIVCKNANEDNVSIEIDD
Sbjct:   38 LAVHSKKIQEVLNWIQKQPLNSNSSNILLLTGPPGSGKTATLYALPNICIHCWNDETQSNIVEDFTDFLLKASRYSELNDEFNNNVGKKLILIKNFPNQFYSEPSSLHHLLVRYKASGKCPLIIIISDSQTLKYSPHWLIPKPLQEELKIDNISFAPIAVTLMKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQDNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLNQNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICEDFSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSCLSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKILPKDYRYSGKWKNFNFLKELEFNKNKSSKYEKLDDKMPVESPDFSQLSFHRKKRDNDEWIVCKNANEDNVSIEIDD 523

HSP 2 Score: 73.559 bits (179), Expect = 1.768e-13
Identity = 36/40 (90.00%), Postives = 38/40 (95.00%), Query Frame = 1 
Query:   70 MNKKRKLDTCASSNSLPFKNKSSVLWIEKYKPKTIT*LFI 189
            MNKKRKLDTCASSNSLPFKNKSSVLWIEKYKPKTI+ L +
Sbjct:    1 MNKKRKLDTCASSNSLPFKNKSSVLWIEKYKPKTISDLAV 40
The following BLAST results are available for this feature:
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RAD171.431e-4731.69RAD17 checkpoint clamp loader component [Source:HG... [more]
RAD172.171e-4731.69RAD17 checkpoint clamp loader component [Source:HG... [more]
RAD174.849e-4731.61RAD17 checkpoint clamp loader component [Source:HG... [more]
RAD174.849e-4731.61RAD17 checkpoint clamp loader component [Source:HG... [more]
RAD174.849e-4731.61RAD17 checkpoint clamp loader component [Source:HG... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hpr-172.410e-2223.79Homolog of S. Pombe Rad; Rad17-like protein [Sour... [more]
hpr-172.410e-2223.79Homolog of S. Pombe Rad; Rad17-like protein [Sour... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rad176.873e-3025.76gene:FBgn0025808 transcript:FBtr0082974[more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rad171.180e-4631.48RAD17 checkpoint clamp loader component [Source:ZF... [more]
rad171.180e-4631.48RAD17 checkpoint clamp loader component [Source:ZF... [more]
rad171.312e-4631.48RAD17 checkpoint clamp loader component [Source:ZF... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
bnip3l1.162e-4934.52BCL2/adenovirus E1B 19kDa interacting protein 3-li... [more]
bnip3l7.874e-4934.94BCL2/adenovirus E1B 19kDa interacting protein 3-li... [more]
bnip3l3.447e-4834.07BCL2/adenovirus E1B 19kDa interacting protein 3-li... [more]
bnip3l5.707e-4733.65BCL2/adenovirus E1B 19kDa interacting protein 3-li... [more]
bnip3l6.681e-4733.65BCL2/adenovirus E1B 19kDa interacting protein 3-li... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rad174.917e-4830.50RAD17 checkpoint clamp loader component [Source:MG... [more]
Rad174.917e-4830.50RAD17 checkpoint clamp loader component [Source:MG... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6NXW6|RAD17_MOUSE3.439e-4730.50Cell cycle checkpoint protein RAD17 OS=Mus musculu... [more]
sp|Q9XT62|RAD17_CHLAE1.560e-4631.61Cell cycle checkpoint protein RAD17 OS=Chlorocebus... [more]
sp|O75943|RAD17_HUMAN2.381e-4631.61Cell cycle checkpoint protein RAD17 OS=Homo sapien... [more]
sp|Q5R652|RAD17_PONAB4.664e-4631.40Cell cycle checkpoint protein RAD17 OS=Pongo abeli... [more]
sp|Q9MBA3|RAD17_ARATH4.655e-2024.61Cell cycle checkpoint protein RAD17 OS=Arabidopsis... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2D0PLZ51.729e-5231.97cell cycle checkpoint protein RAD17 isoform X4 OS=... [more]
A0A3B4D1X82.132e-5132.17RAD17 checkpoint clamp loader component OS=Pygocen... [more]
A0A3B4U1P35.483e-5134.74RAD17 checkpoint clamp loader component OS=Seriola... [more]
A0A3Q4AZM91.700e-5033.47RAD17 checkpoint clamp loader component OS=Mola mo... [more]
W5KBU73.877e-5032.39RAD17 checkpoint clamp loader component OS=Astyana... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rad171.469e-5332.39RAD17 checkpoint clamp loader component [Source:NC... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rad173.451e-4730.90RAD17 checkpoint clamp loader component [Source:ZF... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RAD243.460e-822.92Checkpoint protein; involved in the activation of ... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO344935.885e-4530.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rad174.476e-4630.30RAD17 checkpoint clamp loader component [Source:NC... [more]
back to top
BLAST of RAD17 checkpoint clamp loader component vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000009809.11.768e-13100.00SMESG000009809.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021612 ID=SMED30021612|Name=RAD17 checkpoint clamp loader component|organism=Schmidtea mediterranea sexual|type=transcript|length=1786bp
ATATACATTTTGTTAAATTATTATATTTAATTTAAATTTATTGAGGTTGA
GAAATATTTATTTTGGTTCATGAATAAAAAAAGAAAATTGGATACGTGTG
CCTCATCGAATTCCTTACCTTTTAAAAATAAGTCTAGTGTATTATGGATC
GAAAAATATAAACCTAAAACGATTACCTAGCTGTTCATAGTAAAAAGATT
CAAGAAGTTCTGAATTGGATTCAAAAACAACCTCTTAATTCCAATTCTTC
AAATATTTTGTTATTAACTGGACCTCCTGGTTCAGGTAAAACTGCTACAT
TATACGCGTTGCCTAATATATGTATACATTGTTGGAATGATGAAACTCAA
AGTAATATAGTAGAAGATTTTACAGACTTTCTGCTAAAAGCTTCACGTTA
TTCTGAACTAAATGATGAATTTAATAATAATGTGGGCAAGAAGCTTATAT
TGATTAAAAACTTTCCAAATCAGTTTTACAGTGAACCTAGTTCACTTCAT
CATCTGTTGGTTAGATATAAAGCCTCTGGAAAATGCCCTTTAATAATAAT
CATATCCGATTCCCAAACATTGAAATATTCTCCCCATTGGCTTATTCCAA
AACCTCTGCAAGAGGAACTGAAGATTGATAATATTTCATTTGCTCCTATT
GCTGTAACACTAATGAAGAAAGCCATTTCCAAAATAATCAGACAAGAAAG
ATTTTATATGAACGAAAAAGAGCTGCAAACAATAACGAATCAAATAATCG
AGTCTAGCAACGGCGATATACGAAGTGCCATTAATAACCTAGAGATTTTC
ATTACCATGCAAGATAATAATTCAGCTTTAGTCGACATGAATAAAGATAA
TCCAATTTTCATTTTTCGAGCTCTTGGAAAAATTCTTTATAGTAAAAGAG
AAACCGATGAGCTAGAAATCGCAAAATACAAGGAGAAATTACAACAATTA
TCACAATTAAATCAAAACGAATTTCGACCGCAGCTTCTATTTAATCCTGA
AGCTGTTTTCGATTTTTGCGGCTTGAATTATTCAACATTTGTTAATTATC
TGCACGAAAACCATCTTGACTTTATCGAAGACATCGATCAATTATCTGAA
ATATGTGAAGATTTTTCGTATAGTGACTATCTGTTCAATCGTTGGCTTCC
CGAACAGAATGATTTGTTTGGTCAATATTTAATAAGTACCGTAACTAGGT
CAATGATATATCACAATATTTCCCGACCTAAAAATACTTGGCATCCATTA
CGTAAATCATGTTTAAGTGATATTCAGAAAACGATTTTGCAAACACGACT
TGGTATCGATGAAGTCACTGATTTGTTTCCGAGTTTCATTGGCTTGAACA
ATATTTTGTGTTCGAAATCATTGCTCTCTGAGACATTACCCTACTTAGTG
AAGATTCTCCCCAAAGATTATCGATATTCGGGTAAATGGAAAAATTTTAA
TTTTTTAAAAGAATTAGAATTTAATAAAAACAAAAGTAGTAAATATGAGA
AATTAGATGATAAAATGCCTGTAGAATCACCCGATTTCTCTCAGCTTTCG
TTTCATAGAAAGAAAAGAGACAACGATGAATGGATTGTATGTAAAAATGC
CAATGAGGATAACGTGTCTATTGAGATAGATGATTAGAAATTCCATCTTT
CTATCGGAAAATAGTTTATATTTATTAATATAAATTTCTTTCCAATTATA
TTTATCCTCTGGATTCTCAACATTTTGCTAATGGCAATTTTACTCTCAGT
TCGCAAAATTTAATGAATATAAAAATTTCTTGTATT
back to top

protein sequence of SMED30021612-orf-1

>SMED30021612-orf-1 ID=SMED30021612-orf-1|Name=SMED30021612-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=325bp
MKKAISKIIRQERFYMNEKELQTITNQIIESSNGDIRSAINNLEIFITMQ
DNNSALVDMNKDNPIFIFRALGKILYSKRETDELEIAKYKEKLQQLSQLN
QNEFRPQLLFNPEAVFDFCGLNYSTFVNYLHENHLDFIEDIDQLSEICED
FSYSDYLFNRWLPEQNDLFGQYLISTVTRSMIYHNISRPKNTWHPLRKSC
LSDIQKTILQTRLGIDEVTDLFPSFIGLNNILCSKSLLSETLPYLVKILP
KDYRYSGKWKNFNFLKELEFNKNKSSKYEKLDDKMPVESPDFSQLSFHRK
KRDNDEWIVCKNANEDNVSIEIDD*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0042025host cell nucleus
GO:0005634nucleus
GO:0031389Rad17 RFC-like complex
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR004582Checkpoint_prot_Rad17_Rad24
IPR027417P-loop_NTPase
Vocabulary: biological process
TermDefinition
GO:0000077DNA damage checkpoint
GO:0006281DNA repair
GO:0007049cell cycle
Vocabulary: molecular function
TermDefinition
GO:0003689DNA clamp loader activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 79..99
NoneNo IPR availableGENE3DG3DSA:1.10.8.60coord: 1..64
e-value: 4.9E-7
score: 31.3
NoneNo IPR availablePANTHERPTHR12172:SF0CELL CYCLE CHECKPOINT PROTEIN RAD17coord: 1..284
NoneNo IPR availableCDDcd18140HLD_clamp_RFCcoord: 1..44
e-value: 3.99398E-9
score: 50.2201
IPR004582Checkpoint protein Rad17/Rad24PANTHERPTHR12172CELL CYCLE CHECKPOINT PROTEIN RAD17coord: 1..284