SMED30021601
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021601 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30021601 aligns in the following genomic locations:
Homology
BLAST of SMED30021601 vs. Planmine SMEST
Match: SMESG000003951.1 (SMESG000003951.1) HSP 1 Score: 401.364 bits (1030), Expect = 2.351e-142 Identity = 219/236 (92.80%), Postives = 219/236 (92.80%), Query Frame = 3 Query: 60 MKFVGIIFILFCIKDSCSILSCANFSLKIKENYIIKYHESIANGAELLHVFNNFTACETECCSNPKCSVIVNNFKLHHCYLIHCPKGYICKFVPHANYSIVLKLKRNSEIMEKHFLFTTESISKKIMEKHFLFTTESISKKIMEKHFLFTTESISKNSSGFDSKINGKASTEDVFDSLVNWQXXXXXXXXXXXXXXXXXYGIVYRRKCTMRNKRGIIPIKTKKDGQSDYLINGMYT 767 MKFVGIIFILFCIKDSCSILSCANFSLKIKENYIIKYHESIANGAELLHVFNNFTACETECCSNPKCSVIVNNFKLHHCYLIHCPKGYICKFVPHANYSIVLKLKRNSEIMEKHFLFTTESISKKIMEKHFL FTTESISKNSSG DSKINGKASTEDVFDSLVNWQSVSSVVALVLALFIVLSYGIVYRRKCTMRNKRGIIPIKTKKDGQSDYLINGMYT Sbjct: 1 MKFVGIIFILFCIKDSCSILSCANFSLKIKENYIIKYHESIANGAELLHVFNNFTACETECCSNPKCSVIVNNFKLHHCYLIHCPKGYICKFVPHANYSIVLKLKRNSEIMEKHFLFTTESISKKIMEKHFL----------------FTTESISKNSSGLDSKINGKASTEDVFDSLVNWQSVSSVVALVLALFIVLSYGIVYRRKCTMRNKRGIIPIKTKKDGQSDYLINGMYT 220
BLAST of SMED30021601 vs. Planmine SMEST
Match: SMESG000068862.1 (SMESG000068862.1) HSP 1 Score: 53.5286 bits (127), Expect = 3.951e-8 Identity = 32/90 (35.56%), Postives = 50/90 (55.56%), Query Frame = 3 Query: 129 NFSLKIKENYIIKYHESIANGAELLH--VFNNFTACETECCSNPKCSVIVNNFKLHH-CYLIHCPKGYICKFVPHANYSIVLKLKRNSEI 389 N + I++N II S A GA +H V + F C CCSN C+V V K++H CY+ +C + +C F H+NY ++ +K S++ Sbjct: 22 NRNFSIRKNAIILTKSSKAKGAIFIHKQVISRFADCYALCCSNRDCTVAVYENKVNHKCYIFNCKQPNVCSFTEHSNYISMMYVKPESKV 111
BLAST of SMED30021601 vs. Planmine SMEST
Match: SMESG000068862.1 (SMESG000068862.1) HSP 1 Score: 53.5286 bits (127), Expect = 4.302e-8 Identity = 32/90 (35.56%), Postives = 50/90 (55.56%), Query Frame = 3 Query: 129 NFSLKIKENYIIKYHESIANGAELLH--VFNNFTACETECCSNPKCSVIVNNFKLHH-CYLIHCPKGYICKFVPHANYSIVLKLKRNSEI 389 N + I++N II S A GA +H V + F C CCSN C+V V K++H CY+ +C + +C F H+NY ++ +K S++ Sbjct: 15 NRNFSIRKNAIILTKSSKAKGAIFIHKQVISRFADCYALCCSNRDCTVAVYENKVNHKCYIFNCKQPNVCSFTEHSNYISMMYVKPESKV 104
BLAST of SMED30021601 vs. Planmine SMEST
Match: SMESG000068862.1 (SMESG000068862.1) HSP 1 Score: 54.6842 bits (130), Expect = 4.592e-8 Identity = 39/110 (35.45%), Postives = 59/110 (53.64%), Query Frame = 3 Query: 81 FILFCIKDSCSILSCA----NFSLKIKENYIIKYHESIANGAELLH--VFNNFTACETECCSNPKCSVIVNNFKLHH-CYLIHCPKGYICKFVPHANYSIVLKLKRNSEI 389 I F I S ++LS A N + I++N II S A GA +H V + F C CCSN C+V V K++H CY+ +C + +C F H+NY ++ +K S++ Sbjct: 4 LIFFMI--SLNVLSNADEECNRNFSIRKNAIILTKSSKAKGAIFIHKQVISRFADCYALCCSNRDCTVAVYENKVNHKCYIFNCKQPNVCSFTEHSNYISMMYVKPESKV 111
BLAST of SMED30021601 vs. Planmine SMEST
Match: SMESG000068862.1 (SMESG000068862.1) HSP 1 Score: 53.9138 bits (128), Expect = 8.091e-8 Identity = 36/102 (35.29%), Postives = 56/102 (54.90%), Query Frame = 3 Query: 105 SCSILSCA----NFSLKIKENYIIKYHESIANGAELLH--VFNNFTACETECCSNPKCSVIVNNFKLHH-CYLIHCPKGYICKFVPHANYSIVLKLKRNSEI 389 S ++LS A N + I++N II S A GA +H V + F C CCSN C+V V K++H CY+ +C + +C F H+NY ++ +K S++ Sbjct: 3 SLNVLSNADEECNRNFSIRKNAIILTKSSKAKGAIFIHKQVISRFADCYALCCSNRDCTVAVYENKVNHKCYIFNCKQPNVCSFTEHSNYISMMYVKPESKV 104 The following BLAST results are available for this feature:
BLAST of SMED30021601 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30021601 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30021601 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30021601 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30021601 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021601 ID=SMED30021601|Name=SMED30021601|organism=Schmidtea mediterranea sexual|type=transcript|length=973bpback to top protein sequence of SMED30021601-orf-1 >SMED30021601-orf-1 ID=SMED30021601-orf-1|Name=SMED30021601-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=237bp MKFVGIIFILFCIKDSCSILSCANFSLKIKENYIIKYHESIANGAELLHVback to top Annotated Terms
The following terms have been associated with this transcript:
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