Agrin, putative
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021589 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14Note: Hover over icons to view figure legend
Homology
BLAST of Agrin, putative vs. Ensembl Human
Match: AGRN (agrin [Source:HGNC Symbol;Acc:HGNC:329]) HSP 1 Score: 315.464 bits (807), Expect = 2.455e-86 Identity = 256/907 (28.22%), Postives = 391/907 (43.11%), Query Frame = 1
Query: 205 DLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIM-------YLKKTEQNNFF--------ELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNI----QVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPED--CPLGKMCR-----------NGQCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIRVA-------------PSVI---------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
++++ G V + N + + V V R LKG DL+ + N +++ + C + +G+ I YL +N L +E + +K + F T D C + C FGA C+ P A C C + C + VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TC + C CP + +CG+D Y C++ + +C + +++ G CD C+ + + C + + P+ L Q PVCG D VTY+++C + + + L + G+CQ + D C C + A C+ +CSCD + C +R PVC DG YD+ C+ + C +Q + + C+ +PC GV+C + NG++ CEC + C + PVCG+D VTY S C L TAC + + + G C+ RC + C +C+++ +C CP+ C L + VCG+DG TY SEC + +HAC T+ I+L +A+ G C+ C + C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED C ++CR +G CVC C V VCG+DG Y ECELK+ C + + VA P V GC N A +C CN GS CDP+T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+CKP G C CP G ++ GC+ ++
Sbjct: 44 NVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNK---VVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHF----TPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPS--VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDP------------CSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNP-------RTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGR---DQCPE-PCRFNAVCLSRRGR-PRCSCDRVTCDGAYR-PVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCA--FGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCD---RCGQ----CRFGALCEAE--TGRCVCPSEC--VALAQPVCGSDGHTYPSECMLHVHAC-THQISLHVASAGPCETCGDAVCAFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQ-ELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADA 895 HSP 2 Score: 160.614 bits (405), Expect = 1.308e-38 Identity = 179/652 (27.45%), Postives = 255/652 (39.11%), Query Frame = 1
Query: 493 SVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATC-VTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP------YQYCRI--------DNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEE------PRCKEQGKDCPSYQICKSDLVQAK----CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRF------LEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE-DCPLGKMC----RNGQCVCTD-KCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
+ L S Q Q D C + C+F A C G P C C C + VC DG YDS C ++ EC Q++I ++ G C C ATC V N C+C C S +CG+D +TY + CE++ +C R I+ G CD C +C F +CE E G C CP+ C Q PVCGSD TY SEC L + +C +ISL V G C+T C + C +GA C + QC C C++ PVCG+DG+ Y + C L + +C +Q ++ + CE C + CR +G C+ + P PVCG+D VTY + C L + C +++ L++ G C P P Y C+ ++ A+ CP+ Q C G +Y C C S + + CD+C+ +F I R +PQ DC + +C +C C + R + GP + C E C G C + CVC C + +VCG+DG Y NEC+LK AC I +
Sbjct: 378 GLLLQKVRSGQCQGRDQCPE-PCRFNAVCLSRRGRP---RCSCDRVTCDGAYR--PVCAQDGRTYDSDCWRQQAECRQQRAI-----------PSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCG--QCRFGALCEA-ETGR---CVCPS---ECVALAQ-PVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCET------CGDAVCAFGAVC-----SAGQCVCPR--CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPG--SPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTP-YGCCQDNITAARGVGLAGCPSACQ-------CNPHG-SYGGTCDPATGQC---SCRPGVGGL-RCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTG-LCSCKPGVAGPKC----GQCPDGRAL-----GPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISI 963 HSP 3 Score: 137.117 bits (344), Expect = 1.537e-31 Identity = 157/733 (21.42%), Postives = 275/733 (37.52%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLS---------SLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQS-PCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G C L G CS CP G + ++ E + A F SF ++ + A + L + + G++ Y+ + R D+L + L + V+ R D GSG L S V + G+ H + + R R L +DG G +P L+ +G V G GC+ L I G T+ + + + P + + + P + K CQ + PC C+ E C+C G +G C+ S + N F L L +++ LE+ FL +GLL + D F+S+ L LE G + S E +G W V + + + L + P+ + + +P LN+ +P++ GG F+ + ++ F GAIQ + + + + ++T + G PC PC CVP +C C + + + + + G++ +Y + +K+ N+ + +T + G+ +L + + ++ +AI +G ++++ NLG + VLR T ++ N+W V R R L + + A + ++ L TDG +WLGG + PV P Y T F GC+ ++ V +L+ DA
Sbjct: 1342 GTCQDWALGGGFTCS-CPAG--------RGGAVCEKVLGAPVPAFEGRSFL-AFPTLRAYHTLRLALEFRALEPQGLLLYNGNA-RGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEK--SPCQPN-PCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNL--------------KEPLYVGGAP--DFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLN----GASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTL-AFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGL-VLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRST--VPVNTNRWLRVVAHREQREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDA 2033 HSP 4 Score: 126.331 bits (316), Expect = 3.386e-28 Identity = 147/576 (25.52%), Postives = 211/576 (36.63%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQ---ICKSDLVQAKC-ECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC + C FGA C G A C C VCGSDG+ Y S C ++ C L + IQV+ C R + G C+ ++ C CP++ C + + +CG+D TY + C + +C + G C+ C C F +C + C CP C PVCGSD VTY S C L+ +C ++ + G C+ + C C + C CD+ C++ PVCG+DG+ Y +C L K CE Q ++V C P + + C PY C+ +N + CP Q C N +Y C+ C + +P + + GI + C QG DC CK + KC +CP LG + C D + MR CE + +C + S + +C P CP VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 463 QAPSPCLGVQCAFGATCAVKNGQ---AACECLQA---CSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGA------LCEAETG------------RCVCPSE-CVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDF-SCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQ--DNITAARGVGLAGCPSACQ--C-NPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPD--GRALGPAGCEADASAPATCAEMR---CEFGA------------RCVEESGSAHCVC-------PMLTCPEANATK---VCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQ 970 HSP 5 Score: 64.3142 bits (155), Expect = 2.193e-9 Identity = 84/431 (19.49%), Postives = 161/431 (37.35%), Query Frame = 1
Query: 3367 KPCLNGGLCQT-SSEEMYSCQCLPGYQGITCEEVV-SHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-------------------------NSDIILNNITQWQ----------GPPCGPLQSPCSKTDPEKGFC----VPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PC +GG CQ + ++C C G G CE+V+ + + F+ SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L +++ T G L S G+W+ + + + + L+++ + + LN+++ +F GGV + + G I+ + +N++ + + N+ + GP C +SPC + +P G +PE C C E + + D + F + G S + + K + + F G+L+ + K F+ +A+R+ +E +LG V+R + ++ W V + RN R L + D
Sbjct: 1336 QPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFP--TLRAYHTLRLALEFRALEPQGLLLY--NGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDT--------------DLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPC-QPNPCHGAAPCRVLPEGGAQ-CECPLGREGTFCQTASGQDG--SGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIR--SREPVTLGAWTRVSLERNGRKGALRVGD 1742
BLAST of Agrin, putative vs. Ensembl Human
Match: AGRN (agrin [Source:HGNC Symbol;Acc:HGNC:329]) HSP 1 Score: 311.612 bits (797), Expect = 3.382e-85 Identity = 233/774 (30.10%), Postives = 347/774 (44.83%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNI----QVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPED--CPLGKMCR-----------NGQCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIRVA-------------PSVI---------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
D C + C FGA C+ P A C C + C + VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TC + C CP + +CG+D Y C++ + +C + +++ G CD C+ + + C + + P+ L Q PVCG D VTY+++C + + + L + G+CQ + D C C + A C+ +CSCD + C +R PVC DG YD+ C+ + C +Q + + C+ +PC GV+C + NG++ CEC + C + PVCG+D VTY S C L TAC + + + G C+ RC + C +C+++ +C CP+ C L + VCG+DG TY SEC + +HAC T+ I+L +A+ G C+ C + C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED C ++CR +G CVC C V VCG+DG Y ECELK+ C + + VA P V GC N A +C CN GS CDP+T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+CKP G C CP G ++ GC+ ++
Sbjct: 65 DACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPS--VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDP------------CSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNP-------RTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGR---DQCPE-PCRFNAVCLSRRGR-PRCSCDRVTCDGAYR-PVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCA--FGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCD---RCGQ----CRFGALCEAE--TGRCVCPSEC--VALAQPVCGSDGHTYPSECMLHVHAC-THQISLHVASAGPCETCGDAVCAFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQ-ELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADA 790 HSP 2 Score: 159.844 bits (403), Expect = 1.889e-38 Identity = 179/652 (27.45%), Postives = 255/652 (39.11%), Query Frame = 1
Query: 493 SVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATC-VTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP------YQYCRI--------DNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEE------PRCKEQGKDCPSYQICKSDLVQAK----CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRF------LEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE-DCPLGKMC----RNGQCVCTD-KCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
+ L S Q Q D C + C+F A C G P C C C + VC DG YDS C ++ EC Q++I ++ G C C ATC V N C+C C S +CG+D +TY + CE++ +C R I+ G CD C +C F +CE E G C CP+ C Q PVCGSD TY SEC L + +C +ISL V G C+T C + C +GA C + QC C C++ PVCG+DG+ Y + C L + +C +Q ++ + CE C + CR +G C+ + P PVCG+D VTY + C L + C +++ L++ G C P P Y C+ ++ A+ CP+ Q C G +Y C C S + + CD+C+ +F I R +PQ DC + +C +C C + R + GP + C E C G C + CVC C + +VCG+DG Y NEC+LK AC I +
Sbjct: 273 GLLLQKVRSGQCQGRDQCPE-PCRFNAVCLSRRGRP---RCSCDRVTCDGAYRP--VCAQDGRTYDSDCWRQQAECRQQRAI-----------PSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCG--QCRFGALCEA-ETGR---CVCPS---ECVALAQ-PVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCET------CGDAVCAFGAVC-----SAGQCVCPR--CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPG--SPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTP-YGCCQDNITAARGVGLAGCPSACQ-------CNPHG-SYGGTCDPATGQC---SCRPGVGGL-RCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTG-LCSCKPGVAGPKC----GQCPDGRAL-----GPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISI 858 HSP 3 Score: 137.117 bits (344), Expect = 1.526e-31 Identity = 157/733 (21.42%), Postives = 275/733 (37.52%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLS---------SLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQS-PCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G C L G CS CP G + ++ E + A F SF ++ + A + L + + G++ Y+ + R D+L + L + V+ R D GSG L S V + G+ H + + R R L +DG G +P L+ +G V G GC+ L I G T+ + + + P + + + P + K CQ + PC C+ E C+C G +G C+ S + N F L L +++ LE+ FL +GLL + D F+S+ L LE G + S E +G W V + + + L + P+ + + +P LN+ +P++ GG F+ + ++ F GAIQ + + + + ++T + G PC PC CVP +C C + + + + + G++ +Y + +K+ N+ + +T + G+ +L + + ++ +AI +G ++++ NLG + VLR T ++ N+W V R R L + + A + ++ L TDG +WLGG + PV P Y T F GC+ ++ V +L+ DA
Sbjct: 1237 GTCQDWALGGGFTCS-CPAG--------RGGAVCEKVLGAPVPAFEGRSFL-AFPTLRAYHTLRLALEFRALEPQGLLLYNGNA-RGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCAD--EKSPCQPN-PCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNL--------------KEPLYVGGAP--DFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLN----GASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTL-AFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGL-VLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRST--VPVNTNRWLRVVAHREQREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDA 1928 HSP 4 Score: 125.946 bits (315), Expect = 4.457e-28 Identity = 147/576 (25.52%), Postives = 211/576 (36.63%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQ---ICKSDLVQAKC-ECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC + C FGA C G A C C VCGSDG+ Y S C ++ C L + IQV+ C R + G C+ ++ C CP++ C + + +CG+D TY + C + +C + G C+ C C F +C + C CP C PVCGSD VTY S C L+ +C ++ + G C+ + C C + C CD+ C++ PVCG+DG+ Y +C L K CE Q ++V C P + + C PY C+ +N + CP Q C N +Y C+ C + +P + + GI + C QG DC CK + KC +CP LG + C D + MR CE + +C + S + +C P CP VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 358 QAPSPCLGVQCAFGATCAVKNGQ---AACECLQA---CSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGA------LCEAETG------------RCVCPSE-CVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDF-SCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQ--DNITAARGVGLAGCPSACQ--C-NPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPD--GRALGPAGCEADASAPATCAEMR---CEFGA------------RCVEESGSAHCVC-------PMLTCPEANATK---VCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQ 865 HSP 5 Score: 64.3142 bits (155), Expect = 2.427e-9 Identity = 85/431 (19.72%), Postives = 161/431 (37.35%), Query Frame = 1
Query: 3367 KPCLNGGLCQT-SSEEMYSCQCLPGYQGITCEEVV-SHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-------------------------NSDIILNNITQWQ----------GPPCGPLQSPCSKTDPEKGFC----VPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PC +GG CQ + ++C C G G CE+V+ + + F+ SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L +++ T G L S G+W+ + + + + L+++ + + P G +N + +F GGV + + G I+ + +N++ + + N+ + GP C +SPC + +P G +PE C C E + + D + F + G S + + K + + F G+L+ + K F+ +A+R+ +E +LG V+R + ++ W V + RN R L + D
Sbjct: 1231 QPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFP--TLRAYHTLRLALEFRALEPQGLLLY--NGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGES--PSGTDGLN------------LDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPC-QPNPCHGAAPCRVLPEGGAQ-CECPLGREGTFCQTASGQDG--SGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIR--SREPVTLGAWTRVSLERNGRKGALRVGD 1637
BLAST of Agrin, putative vs. Ensembl Human
Match: AGRN (agrin [Source:HGNC Symbol;Acc:HGNC:329]) HSP 1 Score: 311.227 bits (796), Expect = 4.687e-85 Identity = 233/774 (30.10%), Postives = 347/774 (44.83%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNI----QVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPED--CPLGKMCR-----------NGQCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIRVA-------------PSVI---------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
D C + C FGA C+ P A C C + C + VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TC + C CP + +CG+D Y C++ + +C + +++ G CD C+ + + C + + P+ L Q PVCG D VTY+++C + + + L + G+CQ + D C C + A C+ +CSCD + C +R PVC DG YD+ C+ + C +Q + + C+ +PC GV+C + NG++ CEC + C + PVCG+D VTY S C L TAC + + + G C+ RC + C +C+++ +C CP+ C L + VCG+DG TY SEC + +HAC T+ I+L +A+ G C+ C + C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED C ++CR +G CVC C V VCG+DG Y ECELK+ C + + VA P V GC N A +C CN GS CDP+T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+CKP G C CP G ++ GC+ ++
Sbjct: 32 DACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPS--VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDP------------CSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNP-------RTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGR---DQCPE-PCRFNAVCLSRRGR-PRCSCDRVTCDGAYR-PVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCA--FGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCD---RCGQ----CRFGALCEAET--GRCVCPSEC--VALAQPVCGSDGHTYPSECMLHVHAC-THQISLHVASAGPCETCGDAVCAFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQ-ELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADA 757 HSP 2 Score: 159.458 bits (402), Expect = 2.826e-38 Identity = 179/652 (27.45%), Postives = 255/652 (39.11%), Query Frame = 1
Query: 493 SVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATC-VTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP------YQYCRI--------DNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEE------PRCKEQGKDCPSYQICKSDLVQAK----CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRF------LEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE-DCPLGKMC----RNGQCVCTD-KCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
+ L S Q Q D C + C+F A C G P C C C + VC DG YDS C ++ EC Q++I ++ G C C ATC V N C+C C S +CG+D +TY + CE++ +C R I+ G CD C +C F +CE E G C CP+ C Q PVCGSD TY SEC L + +C +ISL V G C+T C + C +GA C + QC C C++ PVCG+DG+ Y + C L + +C +Q ++ + CE C + CR +G C+ + P PVCG+D VTY + C L + C +++ L++ G C P P Y C+ ++ A+ CP+ Q C G +Y C C S + + CD+C+ +F I R +PQ DC + +C +C C + R + GP + C E C G C + CVC C + +VCG+DG Y NEC+LK AC I +
Sbjct: 240 GLLLQKVRSGQCQGRDQCPE-PCRFNAVCLSRRGRP---RCSCDRVTCDGAYR--PVCAQDGRTYDSDCWRQQAECRQQRAI-----------PSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCG--QCRFGALCEA-ETGR---CVCPS---ECVALAQ-PVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCET------CGDAVCAFGAVC-----SAGQCVCPR--CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPG--SPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTP-YGCCQDNITAARGVGLAGCPSACQ-------CNPHG-SYGGTCDPATGQC---SCRPGVGGL-RCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTG-LCSCKPGVAGPKC----GQCPDGRAL-----GPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISI 825 HSP 3 Score: 127.487 bits (319), Expect = 1.601e-28 Identity = 168/755 (22.25%), Postives = 282/755 (37.35%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLS---------SLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQS-PCSKTDPEKGFCVPEMNETICTC----SPKH--ENLCIRNSMQHDNPA-NTNFFRKYLGKSASKYAA----VKQKRD-------KSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G C L G CS CP G + ++ E + A F SF ++ + A + L + + G++ Y+ + R D+L + L + V+ R D GSG L S V + G+ H + + R R L +DG G +P L+ +G V G GC+ L I G T+ + + + P + + + P + K CQ + PC C+ E C+C G +G C+ S + N F L L +++ LE+ FL +GLL + D F+S+ L LE G + S E +G W V + + + L + P+ + K R +P LN+ +P++ GG F+ + ++ F GAIQ + + + + ++T + G PC PC CVP +C C S H + L +++ D A + F +YL A V + D K+ N+ + +T + G+ +L + + ++ +AI +G ++++ NLG + VLR T ++ N+W V R R L + + A + ++ L TDG +WLGG + PV P Y T F GC+ ++ V +L+ DA
Sbjct: 1204 GTCQDWALGGGFTCS-CPAG--------RGGAVCEKVLGAPVPAFEGRSFL-AFPTLRAYHTLRLALEFRALEPQGLLLYNGNA-RGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEK--SPCQPN-PCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNL--------------KEPLYVGGAP--DFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLN----GASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHSEKALQSNHFELSLRTEATQGL-VLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRST--VPVNTNRWLRVVAHREQREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDA 1918 HSP 4 Score: 125.176 bits (313), Expect = 6.557e-28 Identity = 147/576 (25.52%), Postives = 211/576 (36.63%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQ---ICKSDLVQAKC-ECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC + C FGA C G A C C VCGSDG+ Y S C ++ C L + IQV+ C R + G C+ ++ C CP++ C + + +CG+D TY + C + +C + G C+ C C F +C + C CP C PVCGSD VTY S C L+ +C ++ + G C+ + C C + C CD+ C++ PVCG+DG+ Y +C L K CE Q ++V C P + + C PY C+ +N + CP Q C N +Y C+ C + +P + + GI + C QG DC CK + KC +CP LG + C D + MR CE + +C + S + +C P CP VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 325 QAPSPCLGVQCAFGATCAVKNGQ---AACECLQA---CSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGA------LCEAETG------------RCVCPSE-CVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDF-SCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQ--DNITAARGVGLAGCPSACQ--C-NPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPD--GRALGPAGCEADASAPATCAEMR---CEFGA------------RCVEESGSAHCVC-------PMLTCPEANATK---VCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQ 832 HSP 5 Score: 64.3142 bits (155), Expect = 2.155e-9 Identity = 84/431 (19.49%), Postives = 161/431 (37.35%), Query Frame = 1
Query: 3367 KPCLNGGLCQT-SSEEMYSCQCLPGYQGITCEEVV-SHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-------------------------NSDIILNNITQWQ----------GPPCGPLQSPCSKTDPEKGFC----VPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PC +GG CQ + ++C C G G CE+V+ + + F+ SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L +++ T G L S G+W+ + + + + L+++ + + LN+++ +F GGV + + G I+ + +N++ + + N+ + GP C +SPC + +P G +PE C C E + + D + F + G S + + K + + F G+L+ + K F+ +A+R+ +E +LG V+R + ++ W V + RN R L + D
Sbjct: 1198 QPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFP--TLRAYHTLRLALEFRALEPQGLLLY--NGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDT--------------DLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPC-QPNPCHGAAPCRVLPEGGAQ-CECPLGREGTFCQTASGQDG--SGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIR--SREPVTLGAWTRVSLERNGRKGALRVGD 1604
BLAST of Agrin, putative vs. Ensembl Human
Match: AGRN (agrin [Source:HGNC Symbol;Acc:HGNC:329]) HSP 1 Score: 311.227 bits (796), Expect = 4.863e-85 Identity = 233/774 (30.10%), Postives = 347/774 (44.83%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNI----QVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPED--CPLGKMCR-----------NGQCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIRVA-------------PSVI---------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
D C + C FGA C+ P A C C + C + VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TC + C CP + +CG+D Y C++ + +C + +++ G CD C+ + + C + + P+ L Q PVCG D VTY+++C + + + L + G+CQ + D C C + A C+ +CSCD + C +R PVC DG YD+ C+ + C +Q + + C+ +PC GV+C + NG++ CEC + C + PVCG+D VTY S C L TAC + + + G C+ RC + C +C+++ +C CP+ C L + VCG+DG TY SEC + +HAC T+ I+L +A+ G C+ C + C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED C ++CR +G CVC C V VCG+DG Y ECELK+ C + + VA P V GC N A +C CN GS CDP+T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+CKP G C CP G ++ GC+ ++
Sbjct: 65 DACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPS--VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDP------------CSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNP-------RTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGR---DQCPE-PCRFNAVCLSRRGR-PRCSCDRVTCDGAYR-PVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCA--FGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCD---RCGQ----CRFGALCEAE--TGRCVCPSEC--VALAQPVCGSDGHTYPSECMLHVHAC-THQISLHVASAGPCETCGDAVCAFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQ-ELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADA 790 HSP 2 Score: 159.458 bits (402), Expect = 2.736e-38 Identity = 179/652 (27.45%), Postives = 255/652 (39.11%), Query Frame = 1
Query: 493 SVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATC-VTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP------YQYCRI--------DNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEE------PRCKEQGKDCPSYQICKSDLVQAK----CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRF------LEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE-DCPLGKMC----RNGQCVCTD-KCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
+ L S Q Q D C + C+F A C G P C C C + VC DG YDS C ++ EC Q++I ++ G C C ATC V N C+C C S +CG+D +TY + CE++ +C R I+ G CD C +C F +CE E G C CP+ C Q PVCGSD TY SEC L + +C +ISL V G C+T C + C +GA C + QC C C++ PVCG+DG+ Y + C L + +C +Q ++ + CE C + CR +G C+ + P PVCG+D VTY + C L + C +++ L++ G C P P Y C+ ++ A+ CP+ Q C G +Y C C S + + CD+C+ +F I R +PQ DC + +C +C C + R + GP + C E C G C + CVC C + +VCG+DG Y NEC+LK AC I +
Sbjct: 273 GLLLQKVRSGQCQGRDQCPE-PCRFNAVCLSRRGRP---RCSCDRVTCDGAYR--PVCAQDGRTYDSDCWRQQAECRQQRAI-----------PSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCG--QCRFGALCEA-ETGR---CVCPS---ECVALAQ-PVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCET------CGDAVCAFGAVC-----SAGQCVCPR--CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPG--SPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTP-YGCCQDNITAARGVGLAGCPSACQ-------CNPHG-SYGGTCDPATGQC---SCRPGVGGL-RCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTG-LCSCKPGVAGPKC----GQCPDGRAL-----GPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISI 858 HSP 3 Score: 126.716 bits (317), Expect = 2.338e-28 Identity = 168/755 (22.25%), Postives = 282/755 (37.35%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLS---------SLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQS-PCSKTDPEKGFCVPEMNETICTC----SPKH--ENLCIRNSMQHDNPA-NTNFFRKYLGKSASKYAA----VKQKRD-------KSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G C L G CS CP G + ++ E + A F SF ++ + A + L + + G++ Y+ + R D+L + L + V+ R D GSG L S V + G+ H + + R R L +DG G +P L+ +G V G GC+ L I G T+ + + + P + + + P + K CQ + PC C+ E C+C G +G C+ S + N F L L +++ LE+ FL +GLL + D F+S+ L LE G + S E +G W V + + + L + P+ + K R +P LN+ +P++ GG F+ + ++ F GAIQ + + + + ++T + G PC PC CVP +C C S H + L +++ D A + F +YL A V + D K+ N+ + +T + G+ +L + + ++ +AI +G ++++ NLG + VLR T ++ N+W V R R L + + A + ++ L TDG +WLGG + PV P Y T F GC+ ++ V +L+ DA
Sbjct: 1237 GTCQDWALGGGFTCS-CPAG--------RGGAVCEKVLGAPVPAFEGRSFL-AFPTLRAYHTLRLALEFRALEPQGLLLYNGNA-RGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCAD--EKSPCQPN-PCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNL--------------KEPLYVGGAP--DFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLN----GASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHSEKALQSNHFELSLRTEATQGL-VLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRST--VPVNTNRWLRVVAHREQREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDA 1951 HSP 4 Score: 125.561 bits (314), Expect = 5.577e-28 Identity = 147/576 (25.52%), Postives = 211/576 (36.63%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQ---ICKSDLVQAKC-ECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC + C FGA C G A C C VCGSDG+ Y S C ++ C L + IQV+ C R + G C+ ++ C CP++ C + + +CG+D TY + C + +C + G C+ C C F +C + C CP C PVCGSD VTY S C L+ +C ++ + G C+ + C C + C CD+ C++ PVCG+DG+ Y +C L K CE Q ++V C P + + C PY C+ +N + CP Q C N +Y C+ C + +P + + GI + C QG DC CK + KC +CP LG + C D + MR CE + +C + S + +C P CP VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 358 QAPSPCLGVQCAFGATCAVKNGQ---AACECLQA---CSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGA------LCEAETG------------RCVCPSE-CVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDF-SCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQ--DNITAARGVGLAGCPSACQ--C-NPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPD--GRALGPAGCEADASAPATCAEMR---CEFGA------------RCVEESGSAHCVC-------PMLTCPEANATK---VCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQ 865 HSP 5 Score: 64.3142 bits (155), Expect = 2.667e-9 Identity = 84/431 (19.49%), Postives = 161/431 (37.35%), Query Frame = 1
Query: 3367 KPCLNGGLCQT-SSEEMYSCQCLPGYQGITCEEVV-SHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-------------------------NSDIILNNITQWQ----------GPPCGPLQSPCSKTDPEKGFC----VPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PC +GG CQ + ++C C G G CE+V+ + + F+ SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L +++ T G L S G+W+ + + + + L+++ + + LN+++ +F GGV + + G I+ + +N++ + + N+ + GP C +SPC + +P G +PE C C E + + D + F + G S + + K + + F G+L+ + K F+ +A+R+ +E +LG V+R + ++ W V + RN R L + D
Sbjct: 1231 QPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFP--TLRAYHTLRLALEFRALEPQGLLLY--NGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDT--------------DLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPC-QPNPCHGAAPCRVLPEGGAQ-CECPLGREGTFCQTASGQDG--SGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIR--SREPVTLGAWTRVSLERNGRKGALRVGD 1637
BLAST of Agrin, putative vs. Ensembl Human
Match: EGFLAM (EGF like, fibronectin type III and laminin G domains [Source:HGNC Symbol;Acc:HGNC:26810]) HSP 1 Score: 135.191 bits (339), Expect = 2.454e-31 Identity = 144/661 (21.79%), Postives = 272/661 (41.15%), Query Frame = 1
Query: 2764 NAENYLSINISLVPYSSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-HIWKFKDN----SFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVD-EISYNLVLDA 4722
N+ I + + DG++ Y + + D++++ + R +++R + G+G + S ++ +G H++++ R + L ++ G + + + +G A P L GF GC+ SL + +D+ P + S + + C++GG C + Y C C G++G CE+ + I +F+++ + +P E ++ EITF P + +G+L + D ++ F+S+ L H+E G L S + +G W+ + + + AK L ++ Q I + + GF + N IF GGV N + N LKP F G+IQKI++ND I ++ D + PC +++PC+ G C P C C E L + ++ +++G+S Y + S +++N+ ++FKT + DG+L+ S + + +R+G + + NLG +GV I N + +D +WH V+ +R+ ++ + +DD R L +G +++GG K+ +Y GCIS + + +LV DA
Sbjct: 170 NSYQAFQITLEFRAEAEDGLLLYCGENEHGRGDFMSLAIIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA--PSAYWLVRATGTNRGFQGCVQSLAVNG--------------------------------RRIDMRPWPLGKALSGADVGECSSGICDEASCIHGGTCTAIKADSYICLCPLGFKGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFMEFEITFRPDSGDGVLLYSYDTGSKD--FLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDK--QKIVEGMAEGGFTQIKC------------NTDIFIGGVP-NYDDVKKNSGVLKP-FSGSIQKIILNDRTIHVKH-DFTSGVNVENAAHPC--VRAPCA----HGGSCRPRKEGYDCDCPLGFEGLHCQKAI-----IEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLG--SGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGK-SPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDA 763 HSP 2 Score: 87.8113 bits (216), Expect = 1.124e-16 Identity = 98/400 (24.50%), Postives = 161/400 (40.25%), Query Frame = 1
Query: 2605 CKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLK-----DSFAKSYWSGNAENYLSI---NISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV---SHIWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAK 3759
C + Y +CP+G+ C+ DA L + S+A + W ++YLS I+ P S DG++ Y DT S D+L+I L +VE+R D GSG L+S LT+G H + V R ++ L +D GM T + IG P + + V F G + + ++N +T + DF + V+V EN + PC +GG C+ +E Y C C G++G+ C++ + I +F S++ + + + R R + + F A +GLL + D R N FIS+ L L + GV ++ S G+W+ V ++ +
Sbjct: 346 CTAIKADSYICLCPLGFK--GRHCE---------DAFTLTIPQFRESLRSYAATPWPLEPQHYLSFMEFEITFRPDSGDGVLLYSYDTG-SKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGV--PNYDDVKKNSGVLKPFSGSIQKI-----------ILNDRTIHVKH-----------DFTSGVNV------------ENAA---HPCVRAPCAHGGSCR-PRKEGYDCDCPLGFEGLHCQKAIIEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQ 693
BLAST of Agrin, putative vs. Ensembl Celegans
Match: agr-1 (AGRin (Synaptic protein) homolog; AGRin (Synaptic protein) homolog family member [Source:UniProtKB/TrEMBL;Acc:G5EG68]) HSP 1 Score: 255.373 bits (651), Expect = 2.065e-68 Identity = 196/676 (28.99%), Postives = 308/676 (45.56%), Query Frame = 1
Query: 796 KSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTIC-----------------KCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE--NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLV------QAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETN-SIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVR----RPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSK---VKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYW--NFTENG---CTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVD 2709
++ + C+ CP + C C+C +C + +CG+D ++Y++ C + SC + + R + C+ CE + G G C +CPN + + PVC S V YQS CHL+ +C+ K ++TV + G+C D C +C G C + +C C N+ VCG DG Y N+CFL +C++Q ++ V C+ +PC ++C + C + + + CECP C +++PVC + T+++ C + + +C TK + + + G C C ++ CK V + KC CP+C + VCG+DGKTY +EC ++ AC +I +K + K K +KC FYS C + + +C CP DC + VCG+D TY +EC +K +C +++ + EG C +E C G+ CR+G CVC+ C + R+CG +G Y + C L+ +C I P SH + + SC CN +GS H CD T QC C+ G+ G CD C+ +W + G C C C+ FGS C TGKC CK A+G C +CP G ++ GC ++Y+ D
Sbjct: 147 RTKQAICEQSLCPFGSKCGLKT-GVCEC-KARCRVVTDVVCGSDHVSYSSFCHLSVRSC-----VLAKNGVRLRVATKGPCKKRNPCEDLRCGPGEDCVVNQINGILLAKCVCPTQCPNYGDSVESS---PVCSSHGVDYQSSCHLRHHACESKTNITVKFFGRC------DPCHGHKCPNGQTCQLGVDRRPECKCSEQCTMNSAH--VCGTDGKTYLNECFLKLAACKEQKDILVWKRGNCDEAGSPCEKMEC-GFWGSCVVKPDRTAECECPNR-CEDVMRPVCATNGETFDNECEMKKKSCETKSMIKVKHQGTCGIGV--------CATFDSCKKPQVCVVVDGKPKCVCPSCTDEF--KEVCGSDGKTYSNECRLQNAACMAQKNIFVKYNSACEACKLKKEKCDFYSACVVGENEKAECKCPDDCPSYEMEEGKEVCGTDGVTYSSECHMKKSACHQSKFVMTAFEGKC--------DECLHVQCRYGEECRSGVCVCSYNCPANPPLSARICGENGVLYPSLCHLQLASCQKGAPISEMPP-----SHCHSSKTSFPDSCQCNRVGSFGHTCD-ETGQCKCRPGVAGIKCDHCLPSFWGIHLIAQGALSCRPCGCSAFGSSRSDCEQTTGKCECKNGALGDKCDLCPNGSMMTAGGCVSPAVYKTPRD 778 HSP 2 Score: 219.935 bits (559), Expect = 2.101e-57 Identity = 225/815 (27.61%), Postives = 345/815 (42.33%), Query Frame = 1
Query: 193 IESSDLIIVG-------RVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAK-EFGNGLFEKRISVFLNGATSKQVQNDDP-CSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKS---IQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP-----YPYC----KCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQIC-KSDLVQAKCECP-TCPQSGL--GESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVY---VRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPC---KLQFIKPDEERCPEDCPLGKMC-RNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTA 2541
E+SD+II G RV +L + V RI KG + + E K I ++ I C + + + I L T Q F L+ +F+ ++ A K+ + C + C FG++C T VC C +C+ VCGSD + Y S C + + C L K+ ++V+ C ++ C+ C P CV N C +CPN SS +C + + Y + C ++ +C++ I ++ GRCD C KC C++ D CKC E CT N VCG+D TY +EC LK+ +CK++ + V +G C C ++C + C+ + A+C C C++ R PVC +G +DN+C + K SCE + + V + C C+ Q C + +GK C CP C + VCG+D TY + C L AC+ +K +++ YN C CK + + C Y C + +A+C+CP CP + G+ VCGTDG TY SECHM+ AC + + A G CD+C + +C + C+ C+C +C + +CG + Y + C L++ SC + I+ + C K F PD +C G C GQC C + +K C PS G H + R C C++ GS +C+ +T +C CK G G CD C +G T GC +
Sbjct: 38 FENSDVIIAGVITGISYRVDSLYIENITVIPRRIYKGREYVEENKNI---------VIGNILETEPCAHRLLASDVRIFSL--TNQQGVFLLDAPMIRVSLPIFDALFTISNKQAHRKRRRTKQAICEQSLCPFGSKC-----GLKTGVCEC-KARCRVVTD--VVCGSDHVSYSSFCHLSVRSCVLAKNGVRLRVATKGPCKKRNP------------CEDLRCGPGEDCVVNQINGILLAKCVCPTQCPNYGDSVESSPVCSSHGVDYQSSCHLRHHACESKTNITVKFFGRCDPCHGHKCPNGQTCQLGVDRRPE-CKC---SEQCTMN-SAHVCGTDGKTYLNECFLKLAACKEQKDILVWKRGNCDE--AGSPCEKMECGFWGSCVVKPDRTAECECPN-RCEDVMR-PVCATNGETFDNECEMKKKSCETKSMIKVKHQGTCGIGVCATFDSCKKPQVCVV-VDGKPKCVCPS--CTDEFKEVCGSDGKTYSNECRLQNAACMAQKNIFVKYNSACEA---CKLKKEKCDFYSACVVGENEKAECKCPDDCPSYEMEEGKEVCGTDGVTYSSECHMKKSACHQSKFVM-TAFEGKCDECLHVQCRYGEECRSG-----VCVCSYNCPANPPLSARICGENGVLYPSLCHLQLASCQKGAPISEMPPSHCHSSKTSF--PDSCQCNRVGSFGHTCDETGQCKCRPGVAGIKCDHC--------------------------LPSFWGI--HLIAQGALSCRPCGCSAFGSSRSDCEQTTGKCECKNGALGDKCDLCPNGSM-MTAGGCVS 769 HSP 3 Score: 133.65 bits (335), Expect = 5.780e-31 Identity = 146/547 (26.69%), Postives = 225/547 (41.13%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVK--CEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDP-----VCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPC-PKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGES--VCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDC------VYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPC 2133
PC K+ C F C P TA C C N +C+ + VC ++G +D+ C MK++ C + I+V CG G+ + + + Q C V + P C CP+ C ++CG+D TY+N C ++ +C A + I +Y C+ CK K C+F C + E+ CKCP+ VCG+D VTY SECH+K +C + + ++GKC D C ++QC YG +C C C Y N +P +CG +G++Y + C L SC+K + + + C + S PD Q R+ + G + E + C P + C + ++ I +L L+ +P C ++ +SD Q +C C LG+ +C C + A T C + +C + C + P CICP C + VCGSD TY N C LK+ +C + VS G C
Sbjct: 377 PCEKMECGFWGSCVVKPDR--TAECECPN-RCEDVMR--PVCATNGETFDNECEMKKKSCETKSMIKVKHQGTCGI------GVCATFDSCKKPQVC-----VVVDGKPKCVCPS--CTDEFKEVCGSDGKTYSNECRLQNAACMAQKNIFVKYNSACEACKLKKEKCDFYSACVVGENEKAE-CKCPDDCPSYEMEEGKEVCGTDGVTYSSECHMKKSACHQSKFVMTAFEGKC------DECLHVQCRYGEEC-----RSGVCVCSY----NCPANPPLSARICGENGVLYPSLCHLQLASCQKGAPISEMPPSHCHS---SKTSFPDSCQCNRVGSFGHTCDETGQCKCRPGVAGIKCDHCLPSFWGI-HLIAQGALSCRPC-------------------GCSAFGSSRSDCEQTTGKC-ECKNGALGDKCDLCPNGSMMTAGGC-------------VSPAVYKTPRDCHSLRCFHGAKCVPSPSSFPDCICPQSCNMNHLGIVANMTVCGSDGTTYSNLCELKMFACKHQIDVVPVSMGIC 852 HSP 4 Score: 58.151 bits (139), Expect = 6.317e-8 Identity = 37/121 (30.58%), Postives = 60/121 (49.59%), Query Frame = 1
Query: 4378 VIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGA--KKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNI 4734
++ I G + V +NLG + I+ N ++DN WH+V R R L +D + + D N L ++G ++LGGA KK + KF GC+ L + NL++D+ K+I
Sbjct: 1346 LVFIEEGKLYVGVNLGADVHLKPISTNVTVADNHWHSVSFRRKERKCELWVDSKKILHVVASPGDVN-LDSNGLVYLGGANPKKHKLLKSLNLSNKFVGCVKNLKIFGAEVNLLVDSLKSI 1465
BLAST of Agrin, putative vs. Ensembl Celegans
Match: lam-2 (Laminin-like protein lam-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18823]) HSP 1 Score: 81.2629 bits (199), Expect = 6.864e-15 Identity = 39/95 (41.05%), Postives = 54/95 (56.84%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC 2676
+ C C+ +GS + CD +T QC CK G+TGQ CDRC D ++ F+ NGC C C GSE+ C++ +G+C CK G+ C C GC
Sbjct: 944 QECNCDPLGSEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFSANGCQPCDCEYIGSENQQCDVNSGQCLCKENVEGRRCDQCAENRYGITQGC 1038 HSP 2 Score: 64.3142 bits (155), Expect = 7.768e-10 Identity = 28/57 (49.12%), Postives = 42/57 (73.68%), Query Frame = 1
Query: 2395 SCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGS 2565
+C CN IGSL+ +CD + +C CK G+TG++CD+C+DG+++F+ NGC C C GS
Sbjct: 402 ACGCNEIGSLSTQCD-NEGKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCGCETSGS 457 HSP 3 Score: 64.3142 bits (155), Expect = 8.890e-10 Identity = 32/85 (37.65%), Postives = 46/85 (54.12%), Query Frame = 1
Query: 2431 ECDPSTTQCICKTGITGQYCDRCIDGYWNFTEN-GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCP-IGYSISNHGCK 2679
EC+ QC C + G CD+C G++N T GC C+C+ GSE C++ TG+C CKP G+ C C + S +GC+
Sbjct: 908 ECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSGLGCQECNCDPLGSEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFSANGCQ 992 HSP 4 Score: 59.3066 bits (142), Expect = 2.589e-8 Identity = 33/75 (44.00%), Postives = 42/75 (56.00%), Query Frame = 1
Query: 2461 CKTGITGQYCDRCIDGYWNFT-ENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIG-YSISNHGCK 2679
C+ G +C++CI +W EN C AC CN GS S C+ E GKC CKP G++C C G Y S +GCK
Sbjct: 375 CQGNTQGVHCEQCIANHWRRPGENYCVACGCNEIGSLSTQCDNE-GKCQCKPGVTGRFCDQCLDGFYDFSTNGCK 448
BLAST of Agrin, putative vs. Ensembl Celegans
Match: lam-3 (LAMinin related. See also lmb; Laminin alpha chain [Source:UniProtKB/TrEMBL;Acc:G5ECE3]) HSP 1 Score: 75.8702 bits (185), Expect = 2.806e-13 Identity = 42/99 (42.42%), Postives = 56/99 (56.57%), Query Frame = 1
Query: 2395 SCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGS-ESIVCNIETGKCNCKPLAMGKYCSVCPIGYS--ISNHGCKE 2682
+C CN GS+ +CD + QC CK G+ G+ CD+C Y+NFT+ GC C CN +GS E C+ TGKC C+ G C C GY S GC++
Sbjct: 987 ACDCNDTGSIGSDCDQVSGQCNCKQGVFGKQCDQCRPSYFNFTDAGCQFCHCNIYGSIEDGKCDQTTGKCECRENVEGTMCEKCADGYFNITSGDGCED 1085 HSP 2 Score: 57.7658 bits (138), Expect = 9.719e-8 Identity = 27/75 (36.00%), Postives = 39/75 (52.00%), Query Frame = 1
Query: 2434 CDPSTTQCICKTGITGQYCDRCIDGYWNFTE-NGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGY 2655
CDP C+C G+ C+ C W++ NGC C C+ GS+ +CN TG+C CK +G C +C G+
Sbjct: 1144 CDPIDGSCVCPPNTVGEMCENCTTNAWDYHPLNGCKLCDCSDIGSDGGMCNTFTGQCKCKAAYVGLKCDLCTHGF 1218 HSP 3 Score: 55.0694 bits (131), Expect = 6.776e-7 Identity = 29/75 (38.67%), Postives = 42/75 (56.00%), Query Frame = 1
Query: 2461 CKTGITGQYCDRCIDGYWNFTEN---GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC 2676
CK G TGQYC+ C+ GY+ ++ C+ C C+ GS CN +G+C CKP G+ CS+C ++ N C
Sbjct: 1617 CKPGYTGQYCETCVAGYYGDPQHIGGTCSPCDCHPDGSLHGACNPLSGQCECKPGVTGRTCSMCQERHAFINRVC 1691 HSP 4 Score: 54.299 bits (129), Expect = 9.326e-7 Identity = 32/83 (38.55%), Postives = 43/83 (51.81%), Query Frame = 1
Query: 2398 CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACS-CNRFGSESIVCNIETGKCNCKPLAMGKYCSVC 2643
C C+ GS + C+ T QC+CK G+TG CD C+ ++ T GCT C C G VC+ G C C P +G+ C C
Sbjct: 1086 CGCDPTGSEDVSCNLVTGQCVCKPGVTGLKCDSCLPNFYGLTSEGCTECEPCPAPGQ---VCDPIDGSCVCPPNTVGEMCENC 1165
BLAST of Agrin, putative vs. Ensembl Celegans
Match: F29G6.1 (pep chromosome:WBcel235:X:11502267:11509432:1 gene:WBGene00009257.1 transcript:F29G6.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F29G6.1) HSP 1 Score: 74.7146 bits (182), Expect = 5.510e-13 Identity = 120/507 (23.67%), Postives = 190/507 (37.48%), Query Frame = 1
Query: 889 KCDSSSSQICGTDEITYTNICEMKKVSCK-ANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNI-QCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPY--QYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACL----TKKPLWIIYNGICNEEP-----------------RCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGES--VCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSC------AENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKAC 2310
+CD+S +CGT+ +T+TN C ++K C+ AN IE Y G C C+ N CP+ PVC S T+Q+ CH ++ C + + G T + ++C+ + +C+ C +Y PVC ++G N+C L K+ C ENN +G K Y + CRI+N S + PVC + VT+ ++C + + AC+ KK + + Y G C +P C+ + C + ++ K+ + EC P+ S VC ++G+T+ + C + C +I+ K NI +C C+ S C +PVC S RT+++ C C +N +T +G C CP G CTD+ S +C N Y N C KAC
Sbjct: 790 ECDNSYDPLCGTNGVTFTNACSLQKEICESANSTIEVAYTGMC-------CDTN---------------CPSDFS--------PVCDSKGSTHQNICHFGVKRCIAERTF-----GDVLTIDKFEVCNEVKECNNA------------CPKEY--------SPVCASNGQNIVNECELDKIRC------------LVENNVTTGDKLVKDYDGECCRIENCDIS-----------VFSPVCDTEGVTHANMCLMDQNACIQMKKNKKTIQVSYQGQCCNQPCDEDKTPVCDGTITHPNICRFRIAQCEAERVNKTLSIAYSGECCLLPKGECESSGAVCDSEGQTHMNHCVYQQRRCMAQTISQKTLNIVHTGEC----CALAS-----------------CPKTGQPVCDSRGRTHDSLCHFHNSKCIFDKIHTQNTTLTLDYQGKC---------------CPAG---------CTDELSV----ICDQHENIYRNSCYFNLKAC 1169 HSP 2 Score: 68.5514 bits (166), Expect = 4.702e-11 Identity = 138/560 (24.64%), Postives = 205/560 (36.61%), Query Frame = 1
Query: 892 CDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDIC---------------KFV---------KCEFNGICEMMEDGLGT------ICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCK-KKIS---LTVMYKGKCQTKIQS-DICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEK-QMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKP----LWIIYNGICNEEPRC-----KEQGKDCPS-----YQICKSDLVQAKCECPTCPQSGL-----GE-------------SVCGTDGKTYKSECHMRMHAC---ETNSIALKIANIGT-----CDKCKNKKC----SFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGK---MCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNK 2322
C IC +D TY N+C+ +K C + +E + G+C C FV CE+ + + E G G I C P E C PVC T+ +EC L IE+CK KK+ L V+ +G C+ + + D SN K NT CS ++C N++ DP+CG +G+ + N C L K CE + V + C D N CP PVC + T+++IC+ C+ ++ L I +CNE C KE C S C+ D ++ E L GE VC T+G T+ + C M +AC + N ++++ G CD+ K C + +IC RF QC + V + + Y EC L K E+ SEG + + RC K + G+C C K VC + G +++ C CI +K
Sbjct: 609 CTDDKHPICASDFSTYENLCQFRKQKC-LDSELEVLFKGKCSECLDSPCALPAENSPDESFVCLEDQSTKSLCEYQMLSCIFERGYGVNLTVQYIGVCCPPVESCDTEKPDPVCTDSGATFLTECELNIENCKLKKLDQPKLVVVSQGICEKDMLTDDFSSNRNFQKPNIEFKRENTF-NCS---MECDNSY-DPLCGTNGVTFTNACSLQKEICESANSTIEVAYTGMC------------------CDTN-----------CPSDFSPVCDSKGSTHQNICHFGVKRCIAERTFGDVLTIDKFEVCNEVKECNNACPKEYSPVCASNGQNIVNECELDKIRCLVENNVTTGDKLVKDYDGECCRIENCDISVFSPVCDTEGVTHANMCLMDQNACIQMKKNKKTIQVSYQGQCCNQPCDEDKTPVCDGTITHPNIC---RFRIAQC----EAERVNKTL----SIAYSGECCLLPKGECESSGAVCDSEGQTHMNHCVYQQRRCMAQTISQKTLNIVHTGECCALASCPKTGQPVCDSRGRTHDSLCHFHNSKCIFDK 1122 HSP 3 Score: 57.3806 bits (137), Expect = 1.018e-7 Identity = 108/443 (24.38%), Postives = 169/443 (38.15%), Query Frame = 1
Query: 1096 PNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-----TKIQSDICSNI------QCHYGA-KCIK--------TSNTVAQCS---CDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVI----NSNFC------ENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQC-----ICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKAC 2310
P PD C I+ PVCG+D+VTY + C L+ + L Y G C K+ + IC N CH+ +CI T + +CS C++ +C NT DPVC +G +Y N C CE Q+E I + FC + +GV+ Y D T E V P D T S ++ ++ +I N + + K + P + ++DL +A C C +S + VC T +T+K+ C + AC+ N I + +I C+ +K + + C + P C PT C +++ C + + E + +KSC E QF DE +C CS+ VC N ++N C ++ C
Sbjct: 34 PPPDCDCPSVIR-PVCGTDNVTYNNLCFLRCVQ-RTNEDLLFFYNGTCCDKKECEKVGTPICDNFGETHINDCHFAQFQCIMKKSMGLSLTKLHMGRCSSKDCNH-NCTNTEFDPVCDTNGSVYRNLCVFQMRRCELQLESQRIQLAEDRKFCLRSRETQRFKLAGVESYTSYA----DEPYLETTE------ASTVAP----DLTTTASTTIPMKSEKPSRNETFI--NKVIAKTSASKHRENHSP---LTRNDLNRA-CSIKECDKS--WDPVCDTRNRTHKNVCQFKFFACKINKIDGSVIDIAHSGACRARKSTCIT-CPKDEKKIPICDNRNMTHPTLCSFIQYN-CEARNNEDEERVLVHIKSCHER-----------SPQFTLKDE------------------ICPRTCSRDVKPVCDEANNTHQNLCHFQQYNC 420 HSP 4 Score: 56.6102 bits (135), Expect = 1.952e-7 Identity = 84/368 (22.83%), Postives = 130/368 (35.33%), Query Frame = 1
Query: 1363 RDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE---NNPCS--GVKCPDPYQYCRIDNNGKSTCEC-------------------------PKEPC-PKIVQPVCGNDKVTYESICNLTRTACLTKK----PLWIIYNGICNEEPRCKEQGKDC----PSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVI 2349
+ P+C +D Y+N C K C E+ V+ C ++PC+ PD C D + KS CE P E C + PVC + T+ + C L C KK L ++ GIC ++ + + P+ + + + EC + +CGT+G T+ + C ++ CE+ + +++A G C C T+C PVC S T++N C VK C R V L K E C K C N C K VC ++G NECEL + C+ + ++
Sbjct: 613 KHPICASDFSTYENLCQFRKQKCLDS-ELEVLFKGKCSECLDSPCALPAENSPDESFVCLEDQSTKSLCEYQMLSCIFERGYGVNLTVQYIGVCCPPVESCDTEKPDPVCTDSGATFLTECELNIENCKLKKLDQPKLVVVSQGICEKDMLTDDFSSNRNFQKPNIEFKRENTFNCSMEC-----DNSYDPLCGTNGVTFTNACSLQKEICESANSTIEVAYTGMC-------------------------CDTNCPSDFSPVCDSKGSTHQNICHFGVKRCIAERTFGDV------LTIDKF------EVCNEVKECNNA-------CPKEYSPVCASNGQNIVNECELDKIRCLVENNVTTGDKLV 930
BLAST of Agrin, putative vs. Ensembl Celegans
Match: epi-1 (Laminin-like protein epi-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21313]) HSP 1 Score: 74.7146 bits (182), Expect = 6.294e-13 Identity = 38/88 (43.18%), Postives = 47/88 (53.41%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGY 2655
+ C CN G++ C P QC CK G G +C+ C DGY N T GC C C+ GSE C+ TG+C CKP G C C +GY
Sbjct: 470 KPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTA-GCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGY 556 HSP 2 Score: 72.7886 bits (177), Expect = 2.628e-12 Identity = 36/97 (37.11%), Postives = 52/97 (53.61%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVC-PIGYSISNHGCK 2679
+ C C + + N +C+ QC C+ G TG C+ C GYWN+ E+GC C C S VC++ TG+C+C+ A G C C P I +GC+
Sbjct: 2035 QGCHCGT-AAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQCLPSYLRIPTYGCR 2130 HSP 3 Score: 67.781 bits (164), Expect = 9.837e-11 Identity = 50/200 (25.00%), Postives = 91/200 (45.50%), Query Frame = 1
Query: 2392 RSCLCNSIGSLN--HECDPSTTQCICKTGITGQYCDRCIDGYW---NFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDA----------VELGFLKDSFAKSYWSGNA-----ENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVE-YRVDLGSGAIF 2928
RSC CN G+++ + CD T QC+CK G+ CD+C DG++ ++ + GC +C C+ G+ C+I +G+C C+P G C I NH +++ N+ +A V + FA W G A ++ + I++ + + ++F + + T+ + R+ + V+ A F
Sbjct: 754 RSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCD-----QPIENHYFP--TLWHNQYEAEDAHTEDQKPVRFAVDPEQFADFSWRGYAVFSPIQDKILIDVDITKATVYRLLFRYRNPTSVPVTATVTINPRFTHTHDVEQTGKATF 946 HSP 4 Score: 65.4698 bits (158), Expect = 4.141e-10 Identity = 33/89 (37.08%), Postives = 47/89 (52.81%), Query Frame = 1
Query: 2392 RSCLCNSIGSL--NHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIG 2652
+ C C+ GS N CDP + QC+C+ + G+ CDRC+ G++ F C CSCNR G+ +C+ +C CK G C C G
Sbjct: 1504 QKCGCHPQGSEGGNLVCDPESGQCLCRESMGGRQCDRCLAGFYGFPH--CYGCSCNRAGTTEEICDATNAQCKCKENVYGGRCEACKAG 1590 HSP 5 Score: 59.6918 bits (143), Expect = 2.721e-8 Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = 1
Query: 2398 CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGY 2655
C C+ +G+ CD +T QC+CK G G CDRC ++ + C AC+C+ G S C+ +G+C C G+ C C G+
Sbjct: 564 CNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPN--CKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGF 647 HSP 6 Score: 58.5362 bits (140), Expect = 6.147e-8 Identity = 34/83 (40.96%), Postives = 46/83 (55.42%), Query Frame = 1
Query: 2395 SCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVC 2643
SC C + GS + +C+ QC CK G+ G+ C+RC GY+NF E C C CN G + C+ TG+C C P G+ C C
Sbjct: 1414 SCDCVAQGSESFQCEQYGGQCKCKPGVIGRRCERCAPGYYNFPE--CIKCQCNA-GQQ---CDERTGQCFCPPHVEGQTCDRC 1490 HSP 7 Score: 56.6102 bits (135), Expect = 2.062e-7 Identity = 32/89 (35.96%), Postives = 47/89 (52.81%), Query Frame = 1
Query: 2398 CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTEN-GCTACSCNRFGSE--SIVCNIETGKCNCKPLAMGKYCSVCPIGY 2655
C CN+ +CD T QC C + GQ CDRC+ + + GC C C+ GSE ++VC+ E+G+C C+ G+ C C G+
Sbjct: 1461 CQCNA----GQQCDERTGQCFCPPHVEGQTCDRCVSNAFGYDPLIGCQKCGCHPQGSEGGNLVCDPESGQCLCRESMGGRQCDRCLAGF 1545 HSP 8 Score: 53.9138 bits (128), Expect = 1.549e-6 Identity = 28/84 (33.33%), Postives = 45/84 (53.57%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVC 2643
++C C+ G + ECD ++ QC C TG+ CD+C G++N+ + C C C G++ C+ G+C CK G+ C C
Sbjct: 608 KACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYNYPD--CRGCECLLSGAKGQTCD-SNGQCYCKGNFEGERCDRC 688 HSP 9 Score: 52.7582 bits (125), Expect = 3.272e-6 Identity = 24/77 (31.17%), Postives = 44/77 (57.14%), Query Frame = 1
Query: 2785 INISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVID 3015
+ P S +GIIF T++ +D++ ++L++ V + D GSG + +KS + GR H+I V R + + L++D
Sbjct: 3336 FTFKIRPTSDNGIIFIA--TNKRTDHIAVMLEHGRVVFTYDTGSGQVIIKSDKSIIDGRWHTIKVSRRGKSAHLIVD 3410 HSP 10 Score: 52.373 bits (124), Expect = 3.649e-6 Identity = 27/72 (37.50%), Postives = 43/72 (59.72%), Query Frame = 1
Query: 2455 CICKTGITGQYCDRCIDGYWNFT---ENGCTACSCNRFGSESIV--CNIETGKCNCKPLAMGKYCSVCPIGY 2655
C C+ +TG+ CD+C +W+ E+GC +C CN G+ S + C+++TG+C CK A G+ C C G+
Sbjct: 724 CSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGF 795 HSP 11 Score: 51.6026 bits (122), Expect = 7.208e-6 Identity = 30/83 (36.14%), Postives = 40/83 (48.19%), Query Frame = 1
Query: 2434 CDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKE 2682
C T +C C G+ CD+C GY++ + C C CN G+ VC E G+C CK G +C C GY+ GC E
Sbjct: 438 CAEETGKCECLPRFVGEDCDQCASGYYDAPK--CKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVE 518
BLAST of Agrin, putative vs. Ensembl Fly
Match: SP2353 (gene:FBgn0034070 transcript:FBtr0087209) HSP 1 Score: 102.834 bits (255), Expect = 2.124e-21 Identity = 131/544 (24.08%), Postives = 220/544 (40.44%), Query Frame = 1
Query: 2575 VCNIETGK---CNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDR-SSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVID-GTDSQYGMNPPF--LTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHK----PCLNGGLCQTSSEEMYSCQCLPGYQGITCE-EVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQ----GPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE 4158
CN++ G C + A C +CP G + +GC+E+ + A F K S+ A ++ + I P S DGII + D + D++ ++L +VE+ D GSG ++SR + + +S+++ R D+ LV++ GT Q N F +T + F+ IG TG L+ + +GF GC+ + ++ N+ E P D N D+ + C T K PC +GG C S++ C C G+ G CE + + F +SF+ + L+L++T P +GL+ + G + FI++ LN +E G + S S +G+W+ + I + A+ L ++ + + + GF +++ +Q +F GGV+ KP F G IQ+I IN ++ + +NI PCGPL CVP+M C CS +E
Sbjct: 361 TCNLDCGSDGICALEATAASSRC-LCPFGKT--GNGCQED------IRAHVPRFAKRSWLAFPALHGAYKHVQLRIEFRPESFDGIILLSGERDDLTGDFMALLLNKGFVEFWFDCGSGVGSVRSRETILLNEWNSVIIYRHRWDAWLVLNHGTKVQGRSNGLFSRITFREPVFLGGIGNITG-----LAKRLPLAEGFAGCI-----------RRFVANEHDYKFTEH-------PLGDVINGFDIQD------------------CSTDKCVRYPCQHGGKC-LPSDQGAICLCPIGFVGDLCEIRMDLQVPAFNGSSFLRYAPLGDSALIWLELKVTLKPEQADGLILY--SGPEHRGDFIALYLNDGFVEFAFDLGSGPALVRSEHSLSLGQWHTIKISRTARLAVLKVDKHQEVLT--ISSNGFWHLS------------LDQNLFVGGVNHVDRLPLDLK--YKPFFVGCIQRIDINGHSLGIVAEALGGSNIGNCPHACVARPCGPLAE-----------CVPQMESYECRCSIHNE 824 HSP 2 Score: 100.138 bits (248), Expect = 1.172e-20 Identity = 120/470 (25.53%), Postives = 210/470 (44.68%), Query Frame = 1
Query: 3373 CLNGGLCQTSSEEMYS-CQCLPGYQGITCEE-VVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDI--ILN--NITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKH-ENLC-IRNSMQHDNPA-NTNFFRKY--LGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWE--YNTKFKGCISELYVDEISYNLVLDA--RKNIG 4737
C + G+C + S C C G G C+E + +H+ +F S++ FP+ + ++L I F P + +G++ + D F+++ LNK +E GV + S E+ L+ +WN V+I + +L LNH + ++ R N L SR FR +P+F GG+ + + L F G I++ + N+ + +F + ++N +I C ++ PC G C+P IC C +LC IR +Q PA N + F +Y LG SA + +K + K DG+ +L + F+ + + +G +E A +LG ++R ++S +WHT+++ R R VL +D + +N + L+ D +++GG V+ P + Y F GCI + ++ S +V +A NIG
Sbjct: 366 CGSDGICALEATAASSRCLCPFGKTGNGCQEDIRAHVPRFAKRSWLAFPALHGAYKH-VQLRIEFRPESFDGIILLSGERDDLTGDFMALLLNKGFVEFWFDCGSGVGSVRSRETILLNEWNSVIIYRHRWDAWLVLNHGTK-VQGR--------SNGLFSR--ITFR---EPVFLGGI--GNITGLAKRLPLAEGFAGCIRRFVANEHDYKFTEHPLGDVINGFDIQDCSTDKC--VRYPCQ----HGGKCLPSDQGAICLCPIGFVGDLCEIRMDLQ--VPAFNGSSFLRYAPLGDSALIWLELK-------------VTLKPEQADGL--ILYSGPEHRGDFIALYLNDGFVEFAFDLGSGPALVR--SEHSLSLGQWHTIKISRTARLAVLKVDKHQ-EVLTISSNGFWHLSLDQNLFVGGVNH-VDRLPLDLKYKPFFVGCIQRIDINGHSLGIVAEALGGSNIG 791
BLAST of Agrin, putative vs. Ensembl Fly
Match: SP2353 (gene:FBgn0034070 transcript:FBtr0340019) HSP 1 Score: 102.834 bits (255), Expect = 2.124e-21 Identity = 131/544 (24.08%), Postives = 220/544 (40.44%), Query Frame = 1
Query: 2575 VCNIETGK---CNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDR-SSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVID-GTDSQYGMNPPF--LTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHK----PCLNGGLCQTSSEEMYSCQCLPGYQGITCE-EVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQ----GPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE 4158
CN++ G C + A C +CP G + +GC+E+ + A F K S+ A ++ + I P S DGII + D + D++ ++L +VE+ D GSG ++SR + + +S+++ R D+ LV++ GT Q N F +T + F+ IG TG L+ + +GF GC+ + ++ N+ E P D N D+ + C T K PC +GG C S++ C C G+ G CE + + F +SF+ + L+L++T P +GL+ + G + FI++ LN +E G + S S +G+W+ + I + A+ L ++ + + + GF +++ +Q +F GGV+ KP F G IQ+I IN ++ + +NI PCGPL CVP+M C CS +E
Sbjct: 361 TCNLDCGSDGICALEATAASSRC-LCPFGKT--GNGCQED------IRAHVPRFAKRSWLAFPALHGAYKHVQLRIEFRPESFDGIILLSGERDDLTGDFMALLLNKGFVEFWFDCGSGVGSVRSRETILLNEWNSVIIYRHRWDAWLVLNHGTKVQGRSNGLFSRITFREPVFLGGIGNITG-----LAKRLPLAEGFAGCI-----------RRFVANEHDYKFTEH-------PLGDVINGFDIQD------------------CSTDKCVRYPCQHGGKC-LPSDQGAICLCPIGFVGDLCEIRMDLQVPAFNGSSFLRYAPLGDSALIWLELKVTLKPEQADGLILY--SGPEHRGDFIALYLNDGFVEFAFDLGSGPALVRSEHSLSLGQWHTIKISRTARLAVLKVDKHQEVLT--ISSNGFWHLS------------LDQNLFVGGVNHVDRLPLDLK--YKPFFVGCIQRIDINGHSLGIVAEALGGSNIGNCPHACVARPCGPLAE-----------CVPQMESYECRCSIHNE 824 HSP 2 Score: 100.138 bits (248), Expect = 1.172e-20 Identity = 120/470 (25.53%), Postives = 210/470 (44.68%), Query Frame = 1
Query: 3373 CLNGGLCQTSSEEMYS-CQCLPGYQGITCEE-VVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDI--ILN--NITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKH-ENLC-IRNSMQHDNPA-NTNFFRKY--LGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWE--YNTKFKGCISELYVDEISYNLVLDA--RKNIG 4737
C + G+C + S C C G G C+E + +H+ +F S++ FP+ + ++L I F P + +G++ + D F+++ LNK +E GV + S E+ L+ +WN V+I + +L LNH + ++ R N L SR FR +P+F GG+ + + L F G I++ + N+ + +F + ++N +I C ++ PC G C+P IC C +LC IR +Q PA N + F +Y LG SA + +K + K DG+ +L + F+ + + +G +E A +LG ++R ++S +WHT+++ R R VL +D + +N + L+ D +++GG V+ P + Y F GCI + ++ S +V +A NIG
Sbjct: 366 CGSDGICALEATAASSRCLCPFGKTGNGCQEDIRAHVPRFAKRSWLAFPALHGAYKH-VQLRIEFRPESFDGIILLSGERDDLTGDFMALLLNKGFVEFWFDCGSGVGSVRSRETILLNEWNSVIIYRHRWDAWLVLNHGTK-VQGR--------SNGLFSR--ITFR---EPVFLGGI--GNITGLAKRLPLAEGFAGCIRRFVANEHDYKFTEHPLGDVINGFDIQDCSTDKC--VRYPCQ----HGGKCLPSDQGAICLCPIGFVGDLCEIRMDLQ--VPAFNGSSFLRYAPLGDSALIWLELK-------------VTLKPEQADGL--ILYSGPEHRGDFIALYLNDGFVEFAFDLGSGPALVR--SEHSLSLGQWHTIKISRTARLAVLKVDKHQ-EVLTISSNGFWHLSLDQNLFVGGVNH-VDRLPLDLKYKPFFVGCIQRIDINGHSLGIVAEALGGSNIG 791
BLAST of Agrin, putative vs. Ensembl Fly
Match: CG32354 (gene:FBgn0052354 transcript:FBtr0076662) HSP 1 Score: 99.7525 bits (247), Expect = 8.237e-21 Identity = 121/521 (23.22%), Postives = 196/521 (37.62%), Query Frame = 1
Query: 823 QSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSC-KANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRD-PVCGNDGIIYDNKCFLMKMSCE----KQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNK--KCSFYSICQ-MNRFLEPQCICPTDCVYVRR---PVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLR---VCGTDGNYYENECELKRKACIDNKTIRVAP 2340
+SCPP T P +CG+D + Y NICE++K +C ++ + + C+ K C+ C +D PVCG+D TY + C L+++SC+ ++ + H G C TS C +DC + +D PVC +DG +Y++ C + +C K H ++ C +E C ++ +P CG+D Y S C + + C K ++ + C + R CP+ ECP + VCG+DG Y S C ++M C +++ ++ DKCKN+ +C C+ N ++ + +CG+D +TY NEC L +C R + GPC P ++ C D C++ L VCG+DGN + + CE KR+ C +RV P
Sbjct: 166 RSCPPSITVGAEP-----------------VCGSDGLIYANICELRKKTCSRSGVSLIKDVRDGCERSKGSDCKHR--CSTEKD---------------------PVCGTDGRTYLNRCMLRVQSCRVGLAAVKLS------------------HVGP-CSNTSAVRESCP---VDCNSAPKDGPVCSSDGNVYNSTCEMKLKTCGQGVVKTSRKHCQSTRMC------------------------------RESCWRVARPTCGSDGRLYASPCKMRSSNC--GKHVFEVPLSYCMSQER-HGASDACPT-------------ECPKSDTDSSSQYVCGSDGNIYSSLCELKMLNCGPQRKSIQKVSM---DKCKNRLTRCKQLPPCKDFNSLF--------GSIFSSKRNDKLCGTDAKTYNNECELAHATCL--RGVNLAHIGPC-TDLNSPTKD------------------CGDACTRADLEQQPVCGSDGNTFASMCEFKRRTC----DLRVVP 542 HSP 2 Score: 88.1965 bits (217), Expect = 3.612e-17 Identity = 134/569 (23.55%), Postives = 207/569 (36.38%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKAN-RFIEQQYVGRCDICKFVKCEFNGICEMM-EDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPK-----IVQPVCGNDKVTYESICNLTRTAC-LTKKPLWIIYNGIC-NEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAP 2340
VCGSDG+ Y + C ++++ C S + + K+ +DG +S C+H +C + +CGTD TY N C ++ SC+ ++ +VG C V+ C +DG PVC SD Y S C +K+++C + + T S C C ++ VA+ P CG+DG +Y + C + +C K HV P YC ++ CP E CPK Q VCG+D Y S+C L C +K + + C N RCK Q C + + +K + +CGTD KTY +EC + C + +A+IG C + C T ++PVCGSD T+ + C K ++C + + VS C L C DC ++ VCG+D N Y++EC ++++ C K + V P
Sbjct: 179 VCGSDGLIYANICELRKKTC--------SRSGVSLIKDV-RDGCERSKGSDCKH----------------------RCSTEKDPVCGTDGRTYLNRCMLRVQSCRVGLAAVKLSHVGPCSNTSAVRESCPVDCNSAPKDG--------------------PVCSSDGNVYNSTCEMKLKTCGQGVVKT----------------SRKHCQSTRMCRESCWRVAR--------------PTCGSDGRLYASPCKMRSSNCGK----HVFEV---------------PLSYCMSQERHGASDACPTE-CPKSDTDSSSQYVCGSDGNIYSSLCELKMLNCGPQRKSIQKVSMDKCKNRLTRCK-QLPPCKDFNSLFGSIFSSK----------RNDKLCGTDAKTYNNECELAHATCLR---GVNLAHIGPCTDLNSPTKDCGDAC-------------TRADLEQQPVCGSDGNTFASMCEFKRRTC--DLRVVPVSLKNCAL------TADCESDCD----------------AQPPSFVCGSDNNLYKSECHMRKENC--GKHVFVVP 593
BLAST of Agrin, putative vs. Ensembl Fly
Match: wb (gene:FBgn0261563 transcript:FBtr0302731) HSP 1 Score: 86.6557 bits (213), Expect = 2.071e-16 Identity = 64/192 (33.33%), Postives = 94/192 (48.96%), Query Frame = 1
Query: 2158 EERCPE-DC-PLGKMCRN------GQCVCTDKCSKVKLRVCGTDGNYYENE--------CELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTEN-GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIG-YSISNHGCK 2679
E RCP +C PLG + ++ GQC C + K C DG + N L++ + D+ + P S + +A E+ C C+S+GSL+ +CD T QC C +TG+ CD+C G+WN T GC C C+ GS CN TG+C+CK G++C+ C G + S GC+
Sbjct: 1059 ELRCPSCNCDPLGSLVQDRCDPHTGQCHCKEGVMGAKCHEC-QDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEE----CHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQ 1245 HSP 2 Score: 73.1738 bits (178), Expect = 2.228e-12 Identity = 37/87 (42.53%), Postives = 48/87 (55.17%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIG 2652
R C C+ GS HEC+P T QC CK G+ GQ+C+ C +G++ F+ GC CS R SE VC+ G+C C G C C G
Sbjct: 1197 RDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACR--SEGQVCDPHNGRCICPKFTRGLGCGQCVPG 1281 HSP 3 Score: 62.7734 bits (151), Expect = 4.126e-9 Identity = 31/76 (40.79%), Postives = 44/76 (57.89%), Query Frame = 1
Query: 2434 CDPSTTQCI-CKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYS 2658
C+ +T +CI C+ G +C+RC GYW GC C C+ GSES +C+ G+C CKP G+ C C +GY+
Sbjct: 981 CNVTTGECITCRGNTEGWHCERCKLGYWGDPAVGCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYA 1056 HSP 4 Score: 58.151 bits (139), Expect = 8.155e-8 Identity = 34/89 (38.20%), Postives = 46/89 (51.69%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGS-----ESIVCNIETGKCNCKPLAMGKYCSVC 2643
R C C+ IGS+ +C QC C+ G +G+ CD C GY+ + E C C C+ GS SI C+ G+C CK L +G C C
Sbjct: 1292 RECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPE--CRRCGCDAEGSFTQADGSIACD-SNGQCPCKSLVVGLKCDTC 1377
BLAST of Agrin, putative vs. Ensembl Fly
Match: wb (gene:FBgn0261563 transcript:FBtr0305063) HSP 1 Score: 86.6557 bits (213), Expect = 2.199e-16 Identity = 64/192 (33.33%), Postives = 94/192 (48.96%), Query Frame = 1
Query: 2158 EERCPE-DC-PLGKMCRN------GQCVCTDKCSKVKLRVCGTDGNYYENE--------CELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTEN-GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIG-YSISNHGCK 2679
E RCP +C PLG + ++ GQC C + K C DG + N L++ + D+ + P S + +A E+ C C+S+GSL+ +CD T QC C +TG+ CD+C G+WN T GC C C+ GS CN TG+C+CK G++C+ C G + S GC+
Sbjct: 1059 ELRCPSCNCDPLGSLVQDRCDPHTGQCHCKEGVMGAKCHEC-QDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEE----CHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQ 1245 HSP 2 Score: 73.1738 bits (178), Expect = 2.314e-12 Identity = 37/87 (42.53%), Postives = 48/87 (55.17%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIG 2652
R C C+ GS HEC+P T QC CK G+ GQ+C+ C +G++ F+ GC CS R SE VC+ G+C C G C C G
Sbjct: 1197 RDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACR--SEGQVCDPHNGRCICPKFTRGLGCGQCVPG 1281 HSP 3 Score: 62.3882 bits (150), Expect = 4.332e-9 Identity = 31/76 (40.79%), Postives = 44/76 (57.89%), Query Frame = 1
Query: 2434 CDPSTTQCI-CKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYS 2658
C+ +T +CI C+ G +C+RC GYW GC C C+ GSES +C+ G+C CKP G+ C C +GY+
Sbjct: 981 CNVTTGECITCRGNTEGWHCERCKLGYWGDPAVGCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYA 1056 HSP 4 Score: 58.151 bits (139), Expect = 8.569e-8 Identity = 34/89 (38.20%), Postives = 46/89 (51.69%), Query Frame = 1
Query: 2392 RSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGS-----ESIVCNIETGKCNCKPLAMGKYCSVC 2643
R C C+ IGS+ +C QC C+ G +G+ CD C GY+ + E C C C+ GS SI C+ G+C CK L +G C C
Sbjct: 1292 RECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPE--CRRCGCDAEGSFTQADGSIACD-SNGQCPCKSLVVGLKCDTC 1377
BLAST of Agrin, putative vs. Ensembl Zebrafish
Match: agrn (agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]) HSP 1 Score: 282.337 bits (721), Expect = 3.761e-76 Identity = 236/768 (30.73%), Postives = 334/768 (43.49%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPN----PDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNG----------QCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-----PSVIG----------CISHNYIADEKNKRS------------CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC 2676
D C C FGA C++ +P C C C + VCGSD Y + C +++ +C Q+ I+V C K+ C +C +TC+ + C CP + +CG+D + Y + CE+ +C + I + GRCD C + C + I P PDE+ P+C SD TY+S C ++ + K + L + G C+ K C N C + A C+ N +CSCD + C T++ P+CG DG Y N C C Q ++ V C+ N PC C + + N ++ CEC + CP+ PVCG+D TY S C + C +K + + + G C + E +C IC D +C CP C ++ + VCG+DG TY +EC + + AC T + LK+ G C C + CS+ S C N+ C C +PVCGSD TY N+C L++ SC + R I G C E C + R G +CVC C V VCG+DGN Y ++CELK+ C + V P+++ C Y E NK + C CN GS CDPST QC CK G+ G CDRC G+WNF TEN GCT C+C+ GS C +G C+CKP G C+VCP G + +GC
Sbjct: 167 DGCRGKLCGFGAVCERDQADPSKGECVCKKIVCTS--VVAPVCGSDSSTYSNECELEKAQCNTQRRIKVMRKGPCALKDP------------CSEVTCSFGSTCIQSSDGLSAKCMCPLSCENVPKHVVCGSDGLDYQSECELNMKACATQKNIRVHHQGRCDPCSDTLNSLSTACRVNPKTYQQITFAPAESCPPDEM-------PICASDGHTYESMCQMERTALKNNLELKKVSLGSCKEK---GGCPN-GCKFNAICL-LDNGEFRCSCDPIQCDGTYK-PLCGKDGKTYPNDCERKLQECRTQKDIPVKQQGPCDLNLESPCLKKTC--EFGAVCVVKNSEAVCEC-SDACPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPCAD-----ESCTNCSFGAIC--DAQTGRCVCPKGCLET--RQPVCGSDGTTYDNECKLNVQAC-TKQLDLKVVAHGECKTCGSTVCSWGSQCVNNK-----CECQQCTGQPVKPVCGSDGNTYNNDCELRLASCQKQRKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCELNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQSQGPCPTIVSPPPELVASQHCSQTVYGCCEDNKTAALGVGLAGCPSMCQCNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPCNCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSKMGVNGC 889 HSP 2 Score: 153.68 bits (387), Expect = 8.869e-37 Identity = 161/691 (23.30%), Postives = 290/691 (41.97%), Query Frame = 1
Query: 2725 FLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV-----KPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKP-----CLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFP---SESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEK-GFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
F+ KSY + + Y ++ I++ +S+ GI+ Y+ + D+L++ L YVE R + GSG + S+V + GR H ++V R R++ L +D S G +PP L+ IG + + V+ G VGC+ L + +Y + + ++ S + P P+ + +++ + H P C CQ E Y C+C G +G CE+V + I F +SF+ + + LRQ+ + I L + G++ + D FIS++LN LE G + S E + +WN V + +A++ +++N G V + T +K + +F GG F + ++K F GAIQKI + I ++ + +++ + PCSK E G C P++N C C ++++ + +T + G + +Y K +K+ N + +T + G+ IL + ++ +AI +G +++ +LG K VLR T ++ N W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GC+ ++ VD + +LV DA
Sbjct: 1359 FIPSFGGKSYMAFQTMKAYHTVRIAMEFRASEMTGILLYNGQKSKK-DFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDTDLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVN----NLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYKCECPMGREGRHCEKVSDKGGAFIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIRSKEKIKLNEWNTVNLERASRKGEISIN-------------GKDPVRGEAPNQHTDLNLK-ESLFVGGA--PDFRKVARAAAIKDGFKGAIQKITLMGIPILKADNALSSTDVSMYP-------NHPCSKDVCENGGRCNPQLNSYECACRHGFSGHHCQDTIFEKSAGDTEAI-AFDGHTFIEYHNGVTKSEKALLVNKFELSIRTEATHGL-ILWSGKGVERSDYIALAIVDGRVQMTYDLGSKPVVLRST--VRVNTNHWIRIKASRALRDGSLQVGN---EAAVTGSSPLAATQLDTDGALWLGGLEELSVARRLPKAYSTGFIGCVKDVIVDGVELHLVEDA 2014 HSP 3 Score: 122.479 bits (306), Expect = 3.595e-27 Identity = 154/567 (27.16%), Postives = 223/567 (39.33%), Query Frame = 1
Query: 535 DDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-------TKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP----YQYCR------IDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEE-PRCKEQGKDCPSYQICKSDLVQAKCECPTCPQ-SGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSIC-QMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ PC K C+FGA C AVC CS+ Q + VCGSDG Y S C MK C LQK IQ+ C A + + S C Q+ C CP C + +CG+D TY N C++ +C ++ G C C C + C + C+C + CT PVCGSD TY ++C L++ SC+K+ + V G C + S+ C +C +C C+ L+C+ PVCG+DG Y + C L K CEKQ+++ V + PC + P P Q+C ++N + CP + Q N +Y C+ + C K +P + + GI E C +C + D Q C P G+ +VC K M ++ C+ A K C C F S C ++N + C CP+ DC + VCGSD TY + C LK +C +++ I G CK
Sbjct: 463 ESPCLKKTCEFGAVCVV---KNSEAVCECSDA---CPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPC----ADESCTNCSFGAICDAQT------------GRCVCPKG-CLETRQPVCGSDGTTYDNECKLNVQACTKQLDLKVVAHGECKTCGSTVCSWGSQC------VNNKCEC----QQCTGQPVKPVCGSDGNTYNNDCELRLASCQKQRKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCE-LNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQS-----QGPCPTIVSPPPELVASQHCSQTVYGCCEDNKTAALGVGLAGCPSMCQ---CNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPC--NCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSK-------MGVNGCDKGPKAPK--------SCAELVCHFGSSCVEVNG--QAHCECPSPDCDEKNKTKVCGSDGVTYADRCQLKTIACRQDKEIKVEHLGQCK 968 HSP 4 Score: 121.324 bits (303), Expect = 6.712e-27 Identity = 119/417 (28.54%), Postives = 174/417 (41.73%), Query Frame = 1
Query: 1543 KSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNR------FLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMC--RNGQCVCT---DKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV-----------APSVIGCISHNYIADEKNKRS-CLCNSI---------GSLNH----ECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
K C C K C +V PVCG+D TY + C L + C T++ + ++ G C + C E S I SD + AKC CP ++ VCG+DG Y+SEC + M AC T +++ + G CD C + S + C++N P CP D P+C SD TYE+ C ++ + N + VS G CK ++ CP C +C NG+ C+ +C +CG DG Y N+CE K + C K I V +P + + KN + C C+ GS H C C + I Q+ C D CT CS FG+ +C+ +TG+C C + VC + ++ CK N
Sbjct: 189 KGECVCKKIVCTSVVAPVCGSDSSTYSNECELEKAQCNTQRRIKVMRKGPCALKDPCSEVTCSFGSTCIQSSDGLSAKCMCPLSCENVPKHVVCGSDGLDYQSECELNMKACATQK-NIRVHHQGRCDPCSDTLNSLSTACRVNPKTYQQITFAPAESCPPD----EMPICASDGHTYESMCQMERTALKNNLELKKVSLGSCK------EKGGCPNGCKFNAICLLDNGEFRCSCDPIQCDGTYKPLCGKDGKTYPNDCERKLQECRTQKDIPVKQQGPCDLNLESPCLKKTCEFGAVCVVKNSEAVCECSDACPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPCAD-------ESCTNCS---FGA---ICDAQTGRCVCPKGCLETRQPVCGSDGTTYDNECKLN 581 HSP 5 Score: 70.0922 bits (170), Expect = 2.994e-11 Identity = 90/457 (19.69%), Postives = 180/457 (39.39%), Query Frame = 1
Query: 3367 KPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-NSDIIL---------NN-------------------------ITQWQGPPCGPLQSPCSKTD---PEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDR-MFRAQMLDNNDYNTLATDGWMWLGGA 4617
+PC +GG C+ + ++C C G G CE+V+ + I F S++ F ++++ +++ + F G+L + +G F+S+TL + ++E+ T G +IS G+W+ +V+++ + L++++ P + LN+++ +F GGV + S+ G I+ + +N+ Q N +L NN + + GP C +PC P + +PE C C E R+ + + F + G S + + K ++ I + GM+ + K F+ +++ +G++E +LG V+R + I N+W+TV + R +R + I+ + R + N + L +++GGA
Sbjct: 1324 QPCRHGGTCEEDDND-FTCICPAGRGGAVCEKVIKYFIPSFGGKSYMAF--QTMKAYHTVRIAMEFRASEMTGILLY--NGQKSKKDFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDT--------------DLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVNNLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYK-CECPMGREG---RHCEKVSDKGGA-FIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIR--SKEKIKLNEWNTVNLERASRKGEISINGKDPVRGEA--PNQHTDLNLKESLFVGGA 1752
BLAST of Agrin, putative vs. Ensembl Zebrafish
Match: agrn (agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]) HSP 1 Score: 281.567 bits (719), Expect = 5.929e-76 Identity = 236/768 (30.73%), Postives = 334/768 (43.49%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPN----PDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNG----------QCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-----PSVIG----------CISHNYIADEKNKRS------------CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC 2676
D C C FGA C++ +P C C C + VCGSD Y + C +++ +C Q+ I+V C K+ C +C +TC+ + C CP + +CG+D + Y + CE+ +C + I + GRCD C + C + I P PDE+ P+C SD TY+S C ++ + K + L + G C+ K C N C + A C+ N +CSCD + C T++ P+CG DG Y N C C Q ++ V C+ N PC C + + N ++ CEC + CP+ PVCG+D TY S C + C +K + + + G C + E +C IC D +C CP C ++ + VCG+DG TY +EC + + AC T + LK+ G C C + CS+ S C N+ C C +PVCGSD TY N+C L++ SC + R I G C E C + R G +CVC C V VCG+DGN Y ++CELK+ C + V P+++ C Y E NK + C CN GS CDPST QC CK G+ G CDRC G+WNF TEN GCT C+C+ GS C +G C+CKP G C+VCP G + +GC
Sbjct: 67 DGCRGKLCGFGAVCERDQADPSKGECVCKKIVCTS--VVAPVCGSDSSTYSNECELEKAQCNTQRRIKVMRKGPCALKDP------------CSEVTCSFGSTCIQSSDGLSAKCMCPLSCENVPKHVVCGSDGLDYQSECELNMKACATQKNIRVHHQGRCDPCSDTLNSLSTACRVNPKTYQQITFAPAESCPPDEM-------PICASDGHTYESMCQMERTALKNNLELKKVSLGSCKEK---GGCPN-GCKFNAICL-LDNGEFRCSCDPIQCDGTYK-PLCGKDGKTYPNDCERKLQECRTQKDIPVKQQGPCDLNLESPCLKKTC--EFGAVCVVKNSEAVCEC-SDACPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPCAD-----ESCTNCSFGAIC--DAQTGRCVCPKGCLET--RQPVCGSDGTTYDNECKLNVQAC-TKQLDLKVVAHGECKTCGSTVCSWGSQCVNNK-----CECQQCTGQPVKPVCGSDGNTYNNDCELRLASCQKQRKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCELNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQSQGPCPTIVSPPPELVASQHCSQTVYGCCEDNKTAALGVGLAGCPSMCQCNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPCNCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSKMGVNGC 789 HSP 2 Score: 155.221 bits (391), Expect = 3.463e-37 Identity = 161/691 (23.30%), Postives = 290/691 (41.97%), Query Frame = 1
Query: 2725 FLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV-----KPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKP-----CLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFP---SESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEK-GFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
F+ KSY + + Y ++ I++ +S+ GI+ Y+ + D+L++ L YVE R + GSG + S+V + GR H ++V R R++ L +D S G +PP L+ IG + + V+ G VGC+ L + +Y + + ++ S + P P+ + +++ + H P C CQ E Y C+C G +G CE+V + I F +SF+ + + LRQ+ + I L + G++ + D FIS++LN LE G + S E + +WN V + +A++ +++N G V + T +K + +F GG F + ++K F GAIQKI + I ++ + +++ + PCSK E G C P++N C C ++++ + +T + G + +Y K +K+ N + +T + G+ IL + ++ +AI +G +++ +LG K VLR T ++ N W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GC+ ++ VD + +LV DA
Sbjct: 1259 FIPSFGGKSYMAFQTMKAYHTVRIAMEFRASEMTGILLYNGQKSKK-DFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDTDLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVN----NLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYKCECPMGREGRHCEKVSDKGGAFIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIRSKEKIKLNEWNTVNLERASRKGEISIN-------------GKDPVRGEAPNQHTDLNLK-ESLFVGGA--PDFRKVARAAAIKDGFKGAIQKITLMGIPILKADNALSSTDVSMYP-------NHPCSKDVCENGGRCNPQLNSYECACRHGFSGHHCQDTIFEKSAGDTEAI-AFDGHTFIEYHNGVTKSEKALLVNKFELSIRTEATHGL-ILWSGKGVERSDYIALAIVDGRVQMTYDLGSKPVVLRST--VRVNTNHWIRIKASRALRDGSLQVGN---EAAVTGSSPLAATQLDTDGALWLGGLEELSVARRLPKAYSTGFIGCVKDVIVDGVELHLVEDA 1914 HSP 3 Score: 121.709 bits (304), Expect = 5.754e-27 Identity = 154/567 (27.16%), Postives = 223/567 (39.33%), Query Frame = 1
Query: 535 DDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-------TKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP----YQYCR------IDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEE-PRCKEQGKDCPSYQICKSDLVQAKCECPTCPQ-SGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSIC-QMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ PC K C+FGA C AVC CS+ Q + VCGSDG Y S C MK C LQK IQ+ C A + + S C Q+ C CP C + +CG+D TY N C++ +C ++ G C C C + C + C+C + CT PVCGSD TY ++C L++ SC+K+ + V G C + S+ C +C +C C+ L+C+ PVCG+DG Y + C L K CEKQ+++ V + PC + P P Q+C ++N + CP + Q N +Y C+ + C K +P + + GI E C +C + D Q C P G+ +VC K M ++ C+ A K C C F S C ++N + C CP+ DC + VCGSD TY + C LK +C +++ I G CK
Sbjct: 363 ESPCLKKTCEFGAVCVVKNSE---AVCECSDA---CPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPC----ADESCTNCSFGAICDAQT------------GRCVCPKG-CLETRQPVCGSDGTTYDNECKLNVQACTKQLDLKVVAHGECKTCGSTVCSWGSQC------VNNKCEC----QQCTGQPVKPVCGSDGNTYNNDCELRLASCQKQRKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCE-LNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQS-----QGPCPTIVSPPPELVASQHCSQTVYGCCEDNKTAALGVGLAGCPSMCQ---CNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPC--NCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSK-------MGVNGCDKGPKAPK--------SCAELVCHFGSSCVEVNG--QAHCECPSPDCDEKNKTKVCGSDGVTYADRCQLKTIACRQDKEIKVEHLGQCK 868 HSP 4 Score: 120.939 bits (302), Expect = 9.960e-27 Identity = 119/417 (28.54%), Postives = 174/417 (41.73%), Query Frame = 1
Query: 1543 KSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNR------FLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMC--RNGQCVCT---DKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV-----------APSVIGCISHNYIADEKNKRS-CLCNSI---------GSLNH----ECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
K C C K C +V PVCG+D TY + C L + C T++ + ++ G C + C E S I SD + AKC CP ++ VCG+DG Y+SEC + M AC T +++ + G CD C + S + C++N P CP D P+C SD TYE+ C ++ + N + VS G CK ++ CP C +C NG+ C+ +C +CG DG Y N+CE K + C K I V +P + + KN + C C+ GS H C C + I Q+ C D CT CS FG+ +C+ +TG+C C + VC + ++ CK N
Sbjct: 89 KGECVCKKIVCTSVVAPVCGSDSSTYSNECELEKAQCNTQRRIKVMRKGPCALKDPCSEVTCSFGSTCIQSSDGLSAKCMCPLSCENVPKHVVCGSDGLDYQSECELNMKACATQK-NIRVHHQGRCDPCSDTLNSLSTACRVNPKTYQQITFAPAESCPPD----EMPICASDGHTYESMCQMERTALKNNLELKKVSLGSCK------EKGGCPNGCKFNAICLLDNGEFRCSCDPIQCDGTYKPLCGKDGKTYPNDCERKLQECRTQKDIPVKQQGPCDLNLESPCLKKTCEFGAVCVVKNSEAVCECSDACPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPCAD-------ESCTNCS---FGA---ICDAQTGRCVCPKGCLETRQPVCGSDGTTYDNECKLN 481 HSP 5 Score: 70.8626 bits (172), Expect = 1.632e-11 Identity = 90/457 (19.69%), Postives = 180/457 (39.39%), Query Frame = 1
Query: 3367 KPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-NSDIIL---------NN-------------------------ITQWQGPPCGPLQSPCSKTD---PEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDR-MFRAQMLDNNDYNTLATDGWMWLGGA 4617
+PC +GG C+ + ++C C G G CE+V+ + I F S++ F ++++ +++ + F G+L + +G F+S+TL + ++E+ T G +IS G+W+ +V+++ + L++++ P + LN+++ +F GGV + S+ G I+ + +N+ Q N +L NN + + GP C +PC P + +PE C C E R+ + + F + G S + + K ++ I + GM+ + K F+ +++ +G++E +LG V+R + I N+W+TV + R +R + I+ + R + N + L +++GGA
Sbjct: 1224 QPCRHGGTCEEDDND-FTCICPAGRGGAVCEKVIKYFIPSFGGKSYMAF--QTMKAYHTVRIAMEFRASEMTGILLY--NGQKSKKDFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDT--------------DLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVNNLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYK-CECPMGREG---RHCEKVSDKGGA-FIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIR--SKEKIKLNEWNTVNLERASRKGEISINGKDPVRGEA--PNQHTDLNLKESLFVGGA 1652
BLAST of Agrin, putative vs. Ensembl Zebrafish
Match: agrn (agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]) HSP 1 Score: 276.559 bits (706), Expect = 1.690e-74 Identity = 229/761 (30.09%), Postives = 345/761 (45.34%), Query Frame = 1
Query: 481 EKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCK---CPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVK--CEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCI-KTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDK-------------CKNKKCSFYSICQMNRFLEPQCICPTDC-VYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
++RI V G + + DPCS++ C FG+ C + + +A C C C+ K + VCGSDG+ Y S C + + C QK+I+V H GR + D ++ + C NP Y + P + C IC +D TY ++C+M++ + K N +++ +G C C+FN IC ++E+G C C D + P+CG D TY ++C K++ C+ + + V +G C ++S C C +GA C+ K S V +CS D +DPVCG+DG Y + C + M C Q ++ + + C + C+ + I + C CPK C + QPVCG+D TY++ C L AC + L ++ +G CK G S+ S V KCEC C + + VCG+DG TY ++C +R +C+ I +++A G CD+ C+ +C Y + + +C+C +C + + PVCGSD TY ++C LK C + ++ S+GPC P E V + CS+ C DNKT + + GC S C CN GS CDPST QC CK G+ G CDRC G+WNF TEN GCT C+C+ GS C +G C+CKP G C+VCP G + +GC++
Sbjct: 99 QRRIKVMRKGPCALK----DPCSEVTCSFGSTCIQ-SSDGLSAKCMCP-LSCENVPKHV-VCGSDGLDYQSECELNMKACATQKNIRV---HHQGRCDPCSDTLNSLS------------TACRVNPKTYQQITFAPAESCPPDEMPICASDGHTYESMCQMERTALKNNLELKKVSLGSCKEKGGCPNGCKFNAIC-LLENGE-FRCSC---DPIQCDGTYKPLCGKDGKTYPNDCERKLQECRTQKDIPVKQQGPCDLNLESP-CLKKTCEFGAVCVVKNSEAVCECS----DACPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPCADESCTNC------SFGAICDAQTGRCVCPKG-CLETRQPVCGSDGTTYDNECKLNVQACTKQLDLKVVAHG------ECKTCGSTVCSW---GSQCVNNKCECQQCTGQPV-KPVCGSDGNTYNNDCELRQASCQ-KQIKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCELNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQSQGPCPTIVSPPPE-----------------LVASQHCSQTVY------------------GCCEDNKTAALGVGLAGCPSM-----------CQCNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPCNCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSKMGVNGCEKG 763 HSP 2 Score: 155.221 bits (391), Expect = 3.026e-37 Identity = 161/691 (23.30%), Postives = 290/691 (41.97%), Query Frame = 1
Query: 2725 FLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV-----KPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNG-----GLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFP---SESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEK-GFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
F+ KSY + + Y ++ I++ +S+ GI+ Y+ + D+L++ L YVE R + GSG + S+V + GR H ++V R R++ L +D S G +PP L+ IG + + V+ G VGC+ L + +Y + + ++ S + P P+ + +++ + H PC CQ E Y C+C G +G CE+V + I F +SF+ + + LRQ+ + I L + G++ + D FIS++LN LE G + S E + +WN V + +A++ +++N G V + T +K + +F GG F + ++K F GAIQKI + I ++ + +++ + PCSK E G C P++N C C ++++ + +T + G + +Y K +K+ N + +T + G+ IL + ++ +AI +G +++ +LG K VLR T ++ N W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GC+ ++ VD + +LV DA
Sbjct: 1232 FIPSFGGKSYMAFQTMKAYHTVRIAMEFRASEMTGILLYNGQKSKK-DFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDTDLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVN----NLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYKCECPMGREGRHCEKVSDKGGAFIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIRSKEKIKLNEWNTVNLERASRKGEISIN-------------GKDPVRGEAPNQHTDLNLK-ESLFVGGA--PDFRKVARAAAIKDGFKGAIQKITLMGIPILKADNALSSTDVSMYP-------NHPCSKDVCENGGRCNPQLNSYECACRHGFSGHHCQDTIFEKSAGDTEAI-AFDGHTFIEYHNRVTKSEKALLVNKFELSIRTEATHGL-ILWSGKGVERSDYIALAIVDGRVQMTYDLGSKPVVLRST--VRVNTNHWIRIKASRALRDGSLQVGN---EAAVTGSSPLAATQLDTDGALWLGGLEELSVARRLPKAYSTGFIGCVKDVIVDGVELHLVEDA 1887 HSP 3 Score: 123.635 bits (309), Expect = 1.446e-27 Identity = 156/567 (27.51%), Postives = 223/567 (39.33%), Query Frame = 1
Query: 535 DDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-------TKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP----YQYCR------IDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEE-PRCKEQGKDCPSYQICKSDLVQAKCECPTCPQ-SGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSIC-QMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ PC K C+FGA C AVC CS+ Q + VCGSDG Y S C MK C LQK IQ+ C A + + S C Q+ C CP C + +CG+D TY N C++ +C ++ G C C C + C + C+C + CT PVCGSD TY ++C L+ SC+K+I + V G C + S+ C +C +C C+ L+C+ PVCG+DG Y + C L K CEKQ+++ V + PC + P P Q+C ++N + CP + Q N +Y C+ + C K +P + + GI E C +C + D Q C P G+ +VC K M ++ CE A K C C F S C ++N + C CP+ DC + VCGSD TY + C LK +C +++ I G CK
Sbjct: 334 ESPCLKKTCEFGAVCVV---KNSEAVCECSDA---CPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPC----ADESCTNCSFGAICDAQT------------GRCVCPKG-CLETRQPVCGSDGTTYDNECKLNVQACTKQLDLKVVAHGECKTCGSTVCSWGSQC------VNNKCEC----QQCTGQPVKPVCGSDGNTYNNDCELRQASCQKQIKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCE-LNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQS-----QGPCPTIVSPPPELVASQHCSQTVYGCCEDNKTAALGVGLAGCPSMCQ---CNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPC--NCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSK-------MGVNGCEKGPKAPK--------SCAELVCHFGSSCVEVNG--QAHCECPSPDCDEKNKTKVCGSDGVTYADRCQLKTIACRQDKEIKVEHLGQCK 839 HSP 4 Score: 121.324 bits (303), Expect = 7.008e-27 Identity = 119/417 (28.54%), Postives = 174/417 (41.73%), Query Frame = 1
Query: 1543 KSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNR------FLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMC--RNGQCVCT---DKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV-----------APSVIGCISHNYIADEKNKRS-CLCNSI---------GSLNH----ECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
K C C K C +V PVCG+D TY + C L + C T++ + ++ G C + C E S I SD + AKC CP ++ VCG+DG Y+SEC + M AC T +++ + G CD C + S + C++N P CP D P+C SD TYE+ C ++ + N + VS G CK ++ CP C +C NG+ C+ +C +CG DG Y N+CE K + C K I V +P + + KN + C C+ GS H C C + I Q+ C D CT CS FG+ +C+ +TG+C C + VC + ++ CK N
Sbjct: 60 KGECVCKKIVCTSVVAPVCGSDSSTYSNECELEKAQCNTQRRIKVMRKGPCALKDPCSEVTCSFGSTCIQSSDGLSAKCMCPLSCENVPKHVVCGSDGLDYQSECELNMKACATQK-NIRVHHQGRCDPCSDTLNSLSTACRVNPKTYQQITFAPAESCPPD----EMPICASDGHTYESMCQMERTALKNNLELKKVSLGSCK------EKGGCPNGCKFNAICLLENGEFRCSCDPIQCDGTYKPLCGKDGKTYPNDCERKLQECRTQKDIPVKQQGPCDLNLESPCLKKTCEFGAVCVVKNSEAVCECSDACPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPCAD-------ESCTNCS---FGA---ICDAQTGRCVCPKGCLETRQPVCGSDGTTYDNECKLN 452 HSP 5 Score: 70.8626 bits (172), Expect = 1.547e-11 Identity = 90/457 (19.69%), Postives = 180/457 (39.39%), Query Frame = 1
Query: 3367 KPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-NSDIIL---------NN-------------------------ITQWQGPPCGPLQSPCSKTD---PEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDR-MFRAQMLDNNDYNTLATDGWMWLGGA 4617
+PC +GG C+ + ++C C G G CE+V+ + I F S++ F ++++ +++ + F G+L + +G F+S+TL + ++E+ T G +IS G+W+ +V+++ + L++++ P + LN+++ +F GGV + S+ G I+ + +N+ Q N +L NN + + GP C +PC P + +PE C C E R+ + + F + G S + + K ++ I + GM+ + K F+ +++ +G++E +LG V+R + I N+W+TV + R +R + I+ + R + N + L +++GGA
Sbjct: 1197 QPCRHGGTCEEDDND-FTCICPAGRGGAVCEKVIKYFIPSFGGKSYMAF--QTMKAYHTVRIAMEFRASEMTGILLY--NGQKSKKDFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDT--------------DLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVNNLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYK-CECPMGREG---RHCEKVSDKGGA-FIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIR--SKEKIKLNEWNTVNLERASRKGEISINGKDPVRGEA--PNQHTDLNLKESLFVGGA 1625
BLAST of Agrin, putative vs. Ensembl Zebrafish
Match: agrn (agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]) HSP 1 Score: 276.559 bits (706), Expect = 1.759e-74 Identity = 254/852 (29.81%), Postives = 368/852 (43.19%), Query Frame = 1
Query: 307 KISHENDYHLILVRKIKY-IINCKISI-KSGEDFIMYLKKTEQN-NFFELNYAKEFGNGLFEKRISVFLNGA-TSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPN----PDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNG----------QCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-----PSVIG----------CISHNYIADEKNKRS------------CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
K+S + L L++K K II +S+ +S + ++ + + N + EL Y G L V N A ++ D C C FGA C++ +P C C C + VCGSD Y + C +++ +C Q+ I+V C K+ C +C +TC+ + C CP + +CG+D + Y + CE+ +C + I + GRCD C + C + I P PDE+ P+C SD TY+S C ++ + K + L + G C+ K C N C + A C+ N +CSCD + C T++ P+CG DG Y N C C Q ++ V C+ N PC C + + N ++ CEC + CP+ PVCG+D TY S C + C +K + + + G C + E +C IC D +C CP C ++ + VCG+DG TY +EC + + AC T + LK+ G C C + CS+ S C N+ C C +PVCGSD TY N+C L+ SC + I G C E C + R G +CVC C V VCG+DGN Y ++CELK+ C + V P+++ C Y E NK + C CN GS CDPST QC CK G+ G CDRC G+WNF TEN GCT C+C+ GS C +G C+CKP G C+VCP G + +GC++
Sbjct: 4 KLSARVETVLSLIQKKKAAIIILYVSVDRSSKGHVLNKRVFKLNADLHELLYK---GLLLCPHMTVVLFNAAFLLSKIDPDVCCRGKLCGFGAVCERDQADPSKGECVCKKIVCTS--VVAPVCGSDSSTYSNECELEKAQCNTQRRIKVMRKGPCALKDP------------CSEVTCSFGSTCIQSSDGLSAKCMCPLSCENVPKHVVCGSDGLDYQSECELNMKACATQKNIRVHHQGRCDPCSDTLNSLSTACRVNPKTYQQITFAPAESCPPDEM-------PICASDGHTYESMCQMERTALKNNLELKKVSLGSCKEK---GGCPN-GCKFNAICL-LENGEFRCSCDPIQCDGTYK-PLCGKDGKTYPNDCERKLQECRTQKDIPVKQQGPCDLNLESPCLKKTC--EFGAVCVVKNSEAVCEC-SDACPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPCAD-----ESCTNCSFGAIC--DAQTGRCVCPKGCLET--RQPVCGSDGTTYDNECKLNVQAC-TKQLDLKVVAHGECKTCGSTVCSWGSQCVNNK-----CECQQCTGQPVKPVCGSDGNTYNNDCELRQASCQKQIKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCELNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQSQGPCPTIVSPPPELVASQHCSQTVYGCCEDNKTAALGVGLAGCPSMCQCNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPCNCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSKMGVNGCEKG 807 HSP 2 Score: 154.451 bits (389), Expect = 5.979e-37 Identity = 161/691 (23.30%), Postives = 290/691 (41.97%), Query Frame = 1
Query: 2725 FLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV-----KPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKP-----CLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFP---SESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEK-GFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
F+ KSY + + Y ++ I++ +S+ GI+ Y+ + D+L++ L YVE R + GSG + S+V + GR H ++V R R++ L +D S G +PP L+ IG + + V+ G VGC+ L + +Y + + ++ S + P P+ + +++ + H P C CQ E Y C+C G +G CE+V + I F +SF+ + + LRQ+ + I L + G++ + D FIS++LN LE G + S E + +WN V + +A++ +++N G V + T +K + +F GG F + ++K F GAIQKI + I ++ + +++ + PCSK E G C P++N C C ++++ + +T + G + +Y K +K+ N + +T + G+ IL + ++ +AI +G +++ +LG K VLR T ++ N W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GC+ ++ VD + +LV DA
Sbjct: 1276 FIPSFGGKSYMAFQTMKAYHTVRIAMEFRASEMTGILLYNGQKSKK-DFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDTDLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVN----NLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYKCECPMGREGRHCEKVSDKGGAFIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIRSKEKIKLNEWNTVNLERASRKGEISIN-------------GKDPVRGEAPNQHTDLNLK-ESLFVGGA--PDFRKVARAAAIKDGFKGAIQKITLMGIPILKADNALSSTDVSMYP-------NHPCSKDVCENGGRCNPQLNSYECACRHGFSGHHCQDTIFEKSAGDTEAI-AFDGHTFIEYHNRVTKSEKALLVNKFELSIRTEATHGL-ILWSGKGVERSDYIALAIVDGRVQMTYDLGSKPVVLRST--VRVNTNHWIRIKASRALRDGSLQVGN---EAAVTGSSPLAATQLDTDGALWLGGLEELSVARRLPKAYSTGFIGCVKDVIVDGVELHLVEDA 1931 HSP 3 Score: 123.25 bits (308), Expect = 1.742e-27 Identity = 156/567 (27.51%), Postives = 223/567 (39.33%), Query Frame = 1
Query: 535 DDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-------TKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP----YQYCR------IDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEE-PRCKEQGKDCPSYQICKSDLVQAKCECPTCPQ-SGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSIC-QMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ PC K C+FGA C AVC CS+ Q + VCGSDG Y S C MK C LQK IQ+ C A + + S C Q+ C CP C + +CG+D TY N C++ +C ++ G C C C + C + C+C + CT PVCGSD TY ++C L+ SC+K+I + V G C + S+ C +C +C C+ L+C+ PVCG+DG Y + C L K CEKQ+++ V + PC + P P Q+C ++N + CP + Q N +Y C+ + C K +P + + GI E C +C + D Q C P G+ +VC K M ++ CE A K C C F S C ++N + C CP+ DC + VCGSD TY + C LK +C +++ I G CK
Sbjct: 378 ESPCLKKTCEFGAVCVV---KNSEAVCECSDA---CPQDQDPVCGSDGHTYSSSCQMKAMGCALQKQIQMQHKGPC----ADESCTNCSFGAICDAQT------------GRCVCPKG-CLETRQPVCGSDGTTYDNECKLNVQACTKQLDLKVVAHGECKTCGSTVCSWGSQC------VNNKCEC----QQCTGQPVKPVCGSDGNTYNNDCELRQASCQKQIKIEVAKPGVCDEDCGSGGSGSGSETCEQDRCRRYGGTWDDDMEDERCVCE-LNCQIVLHSPVCGSDGNTYSSDCELKKTRCEKQIDLSVQS-----QGPCPTIVSPPPELVASQHCSQTVYGCCEDNKTAALGVGLAGCPSMCQ---CNHYGSYGGTCDPSTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTENMSGCTPC--NCDGVGSVRDDCEQMSGLCSCKPGVKGMKCNVCPDGSK-------MGVNGCEKGPKAPK--------SCAELVCHFGSSCVEVNG--QAHCECPSPDCDEKNKTKVCGSDGVTYADRCQLKTIACRQDKEIKVEHLGQCK 883 HSP 4 Score: 70.4774 bits (171), Expect = 2.158e-11 Identity = 90/457 (19.69%), Postives = 180/457 (39.39%), Query Frame = 1
Query: 3367 KPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ-NSDIIL---------NN-------------------------ITQWQGPPCGPLQSPCSKTD---PEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDR-MFRAQMLDNNDYNTLATDGWMWLGGA 4617
+PC +GG C+ + ++C C G G CE+V+ + I F S++ F ++++ +++ + F G+L + +G F+S+TL + ++E+ T G +IS G+W+ +V+++ + L++++ P + LN+++ +F GGV + S+ G I+ + +N+ Q N +L NN + + GP C +PC P + +PE C C E R+ + + F + G S + + K ++ I + GM+ + K F+ +++ +G++E +LG V+R + I N+W+TV + R +R + I+ + R + N + L +++GGA
Sbjct: 1241 QPCRHGGTCEEDDND-FTCICPAGRGGAVCEKVIKYFIPSFGGKSYMAF--QTMKAYHTVRIAMEFRASEMTGILLY--NGQKSKKDFLSLTLVEGYVELRFNTGSGTASVISKVLVKPGRWHQLVVVRNRRNAMLSVDNEPSVEGQSPPGTDGLNLDT--------------DLFIGGVLDDMSADVKERTSVSAGLVGCIRMLDVNNLVYNLQENGGSVLYGSAIGECGNNPCLPNPCKNGASCQVKEAEIFHCKCVNGFSGPTCADAHNPCDPNKCHPPSRCQVLPEGGYK-CECPMGREG---RHCEKVSDKGGA-FIPYFTGDSFLELKGLHTYNQDLRQKFSMTIVLLANDSKGMIFYNGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIR--SKEKIKLNEWNTVNLERASRKGEISINGKDPVRGEA--PNQHTDLNLKESLFVGGA 1669
BLAST of Agrin, putative vs. Ensembl Zebrafish
Match: EGFLAM (EGF like, fibronectin type III and laminin G domains [Source:NCBI gene;Acc:799405]) HSP 1 Score: 125.176 bits (313), Expect = 2.904e-28 Identity = 145/664 (21.84%), Postives = 269/664 (40.51%), Query Frame = 1
Query: 2764 NAENYLSINISLVPYSSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEE-VVSHIWKFKDNSF----VIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNL-NHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCS----PKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDN-NDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVL 4716
N+ I++ + DG++ Y + + D+ ++ L + YR + G+GA + S + IG+ H++ + R + L +D + T + +G A P L GF GC+ SL + + ++D+ P S + + + C NGG+C + + + C C GY+G CEE + + +F + F +P S ++ E+TF P + +G L + D R+ F+S++L ++E G T + S E+ + W+ + + + AK L + N P V + TQ + + P++ GGV N + + L+P + G++QKI +ND I N+ PC + +PC+ G C + +E C C+ KH + S++ ++ G+S Y + S ++ N+ ++FK+ S DG+L+ + S + + +++G + + NLG +G + N SD KWH V+ +R+ ++ L +DD + AQ + L +G +++GG K+ +Y GCIS + Y+L L
Sbjct: 324 NSYQSFQISLEFKAEAEDGLLLYCGENEHGRGDFTSLALIRGKLHYRFNCGTGAAQIISDSSIVIGQWHTVTIFRDGMNGWLRLDNDTPVSSRSQGQYTKITFRTALFLGGA--PNGYWLVRAVGTNRGFQGCMQSLTVNS--------------------------------KAIDIRPWPKGNALSGADIGECSNSVCEKVTCANGGICFANRADGFICLCPLGYKGFLCEESFLLSLPQFNQSMFSYASAPWPQSSHNYLSFMEFEVTFQPTSPDGTLLYTDDATSRD--FLSISLVAGYVEFRFDCGSGATVIRSEEAIAMNVWHELRVSRTAKNGILQVDNQRP--------------VEGMAEGAFTQIKCSS-PLYIGGV-PNYDITKPSAGILRP-YSGSVQKITLNDRGIELNPGQAFGVNVLN-AAHPC--VDNPCAN----GGTCRAKWDEYDCDCALGYDGKHCQKAVTESIE---------IPQFTGRSYLTYDNRDILKRISGSRTNLFMRFKSTSKDGLLLWRGESPMRANSDYLSLGLQDGSLIFSYNLG--SGAATVVINGTFSDGKWHRVKAVRDGQSGKLTVDD--YGAQTGRSPGKMRQLNINGDLYIGGMKEIALHTNRQYMRGLVGCISHFTL-ATDYHLSL 913 HSP 2 Score: 81.6481 bits (200), Expect = 6.506e-15 Identity = 91/384 (23.70%), Postives = 156/384 (40.62%), Query Frame = 1
Query: 2638 VCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSI---NISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS---HIWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAK 3759
+CP+GY C+E+ + + + S+A + W ++ NYLS ++ P S DG + Y +D S D+L+I L YVE+R D GSGA ++S + + H + V R ++ L +D GM A FT + + P+ D+T G L+ +G KI L ++ + P Q+ N+ + PC NGG C+ +E Y C C GY G C++ V+ I +F S++ + + + R R L + F + +GLL + + R N ++S+ L L + G ++ + GKW+ V ++ +
Sbjct: 511 LCPLGYK--GFLCEESFL----LSLPQFNQSMFSYASAPWPQSSHNYLSFMEFEVTFQPTSPDGTLLY-TDDATSRDFLSISLVAGYVEFRFDCGSGATVIRSEEAIAMNVWHELRVSRTAKNGILQVDNQRPVEGM---------AEGAFTQIKCSSPLYIGGVPNYDITKPSAGI---LRPYSGSVQKITLNDRGI-------ELNPG--------------------QAFGVNVLNAAHPCVDNPCANGGTCRAKWDE-YDCDCALGYDGKHCQKAVTESIEIPQFTGRSYLTYDNRDILKRISGSRTNLFMRFKSTSKDGLLLWRGESPMRANSDYLSLGLQDGSLIFSYNLGSGAATVVINGTFSDGKWHRVKAVRDGQ 847
BLAST of Agrin, putative vs. Ensembl Xenopus
Match: sos1 (SOS Ras/Rac guanine nucleotide exchange factor 1 [Source:Xenbase;Acc:XB-GENE-1011399]) HSP 1 Score: 308.916 bits (790), Expect = 2.889e-84 Identity = 270/935 (28.88%), Postives = 407/935 (43.53%), Query Frame = 1
Query: 202 SDLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFE----------------KRISVFLNGAT-SKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQV------------PVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE---NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQ-------FIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVAP--------------------SVIGCISHNYIAD-----EKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGF 2727
+++++ G V + N + + V V R LKG +++ + N ++V C + +G+ I ++ ++ + + L +R+S G + Q D C + C FGA C+K +P A C C T C + VCGSD Y + C ++R +C Q+ I+V CG K+ C SC +TCV + C CP + +CG+D Y N C++ K +C + +++ GRCD C+ N C + NP T+ +++ PVC D VTY ++C + + I L ++ G+CQ + D C C + + C+ V +CSCD + C +T++ PVCG D Y N C K C ++ + V + C+ +PC C + + N K+ CEC ++ C + PVCG+DK TY + C L TAC KK + I++ G C+ RC + C IC+++ +C CPT C S + VCGTDG TY+SEC + + AC T I L++A G C C N C+F + C N QC+C T PVCGS+ TY+N+C LK SC + +LI G C+ + ++ C +D CR + +CVC C V ++ VCG+DG Y NECELK+ C + + VA +V GC N A SC C+ GS CDP+T QC CK G+ G CDRC G+WNF ++GCT C+C+ G+ C TG C+CK G CS CP G GC+++ DA++ F
Sbjct: 42 ANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNK---VMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACAS--IVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDP------------CSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRV------------NPR---TRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQ---DKCKE-DCKHNSVCLNRRG-VTRCSCDRVTCDSTYK-PVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCS--FGAACVVKNKKAVCEC-QQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCD---RCGK----CQFGAICEAET--GRCVCPTECVPSA--QPVCGTDGNTYESECELHVRAC-TQQINLQVAAQGDCKTCGNTLCTFGAKCVNN-----QCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQD-----RCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQF 913 HSP 2 Score: 155.221 bits (391), Expect = 5.044e-37 Identity = 175/759 (23.06%), Postives = 301/759 (39.66%), Query Frame = 1
Query: 2725 FLKDSFAKSYWSGNA-ENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITC---------------------------------------EEVVSHIWKF----KDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKI------LINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTC-----SPKHENLCIRNSMQHDNPANTN---FFRKYLGKSASKY-------AAVKQKRDKSSTK---NNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLI--DDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGF--PWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ KSY + + Y ++ I++ SS+ G++ Y+ + D++++ + N +VE R + GSG + S+V + G+ H ++V R R L +DG G +P L+ IG A + +S ++ G GC+ L + ++Y + + + ++ S V + +P CQ + PC NG CQ EM+ CQCL GY G TC E+V + + N + + LQ L+ +L +E+ FL NGL+ + D F+S++L+ +LE G + S E + W V + ++ + + +N+ Q K R P LN+ +P+F GG + + + S +F GAIQKI ++ +ENIR S I I+ ++ PC PC G C P C C + E + I + + + + ++ + S+ V D S N + KT + G+++ K +A+ G +E+ +LG K VLR T ++ N+W ++ +R+NR+ +L + +D + + + L TDG +WLGG +K + P Y+T F GC+ ++ VD+ +LV DA N PIL C
Sbjct: 1411 FIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQS-LGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVN----NQLYNLQEGGADVLYGSGVG------------ECGNNP----------------CQPN-PCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLK--------------EPLFVGGAPDFSKLARAAAAST--SFEGAIQKISIKGLPVLREENIR---SAI---EISTFRSHPCTKKPFPCQN----GGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYI-ALAVVGGYVEMTYDLGSKPVVLRST--VPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKE---LDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDAL-NKPPILYC 2102 HSP 3 Score: 117.857 bits (294), Expect = 1.325e-25 Identity = 154/568 (27.11%), Postives = 215/568 (37.85%), Query Frame = 1
Query: 526 VQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK----------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE----NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPRCKEQGK---DCPSYQICKSDLVQAK--CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ-MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ + PC C FGA C AVC C CQA VCGSD Y + C ++ C L+K I + C R Q G C+ ++ C CP + C S+ +CGTD TY + CE+ +C ++ G C C C F C + C C C + +PVCGS+ VTY ++C LK SCK+ + V G C+ + + C +C +C CD+ C R VCG+DGI Y N+C L K CEK+ +++V C ++P C C DN + CP Q + +Y C+ T C K +P + + GI + K+ G +C + D Q C C T SGL S C DG+ + CE + A K C KC F + C + F +C P VCGSDD TY + C LK +C + R IT GPC+
Sbjct: 468 LHSPSPCLNTTCSFGAACVV---KNKKAVCECQQV-CQAVYD--PVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGA------ICEAET------------GRCVCPTE-CVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKC------VNNQCVCLT----CERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFT-CHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNI-TAALGVGLAGCPSSCQ---CHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTD----KKSGCTPCNCDPVGAVRDDCEQMTGLCSCKT-GISGLKCSQC-PDGR------KPGLTGCEKDPSAPK--------SCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQ 975 HSP 4 Score: 69.3218 bits (168), Expect = 7.358e-11 Identity = 88/437 (20.14%), Postives = 175/437 (40.05%), Query Frame = 1
Query: 3355 CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDE--NIRFQNSDIILNN---------------------------------ITQWQGPPCGPLQSPCSKTDP------EKGFCVPEMNETICTCSPKHEN-LCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
C++H PCL+GG C+ + ++ ++C C G G CE+ + + I F S++ F + ++ +++ + F NGLL + +G FIS+ +N +E+ T G + S GKW+ +V+ + + L+++ G +VN + +F GG + S SL G I+++ +N++ N++ +D++ + ++ + GP C ++PC DP +PE C C E C + S Q N F ++ G S + ++ K + + F ++ +G++ + K F+ +++ +G +E +LG G I + I N W +V + R+ R ++ I++
Sbjct: 1373 CESH-PCLHGGTCEDNGKD-FTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAF--KMMKAYHTVRIAMEFRSSEQNGLLLY--NGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVD-------------GESHVNGESPSGTDGLNLDTD-LFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPC---DPNPCHVSATCLVLPEGGAK-CECPMGREGEFCEKVSNQD---LNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLG--KGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINN 1780 HSP 5 Score: 67.0106 bits (162), Expect = 3.211e-10 Identity = 88/443 (19.86%), Postives = 161/443 (36.34%), Query Frame = 1
Query: 2560 GSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVD--AVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFL----TNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIF------------------------PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILK 3750
G C E C+ P + C V P G + + KV + L FL SY ++ + ++ L++ + + + +G+IFY+ TD D++++ L + +EYR DLG GA +KS+ ++ + S+ +ER R + I+ + G +P T L+ +G A P + L+ + F G + + I + V+ + + E + + PC NGGLC + ++ Y C C G+ G CE+V S F +++ + PSE L + +L I A GL+ + G +R +I++ + ++E+T L S +W H+ ++
Sbjct: 1617 GYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGA--PDFSKLARAAAASTSFEGAIQKISI----------------------------------KGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLC-SPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKT--EATQGLILWSGKGTERA-YYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVR 2019 HSP 6 Score: 56.9954 bits (136), Expect = 3.945e-7 Identity = 30/77 (38.96%), Postives = 44/77 (57.14%), Query Frame = 1
Query: 1021 CKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKI 1251
C +KC+F C ++ G C+CP+P LC + VCGSDDVTY C LK +C++ ++TV + G CQ +
Sbjct: 906 CDAIKCQFGATCVVI--GGFAHCECPSP--LCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETV 978
BLAST of Agrin, putative vs. Ensembl Xenopus
Match: sos1 (SOS Ras/Rac guanine nucleotide exchange factor 1 [Source:Xenbase;Acc:XB-GENE-1011399]) HSP 1 Score: 308.531 bits (789), Expect = 3.786e-84 Identity = 270/935 (28.88%), Postives = 407/935 (43.53%), Query Frame = 1
Query: 202 SDLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFE----------------KRISVFLNGAT-SKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQV------------PVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE---NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQ-------FIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVAP--------------------SVIGCISHNYIAD-----EKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGF 2727
+++++ G V + N + + V V R LKG +++ + N ++V C + +G+ I ++ ++ + + L +R+S G + Q D C + C FGA C+K +P A C C T C + VCGSD Y + C ++R +C Q+ I+V CG K+ C SC +TCV + C CP + +CG+D Y N C++ K +C + +++ GRCD C+ N C + NP T+ +++ PVC D VTY ++C + + I L ++ G+CQ + D C C + + C+ V +CSCD + C +T++ PVCG D Y N C K C ++ + V + C+ +PC C + + N K+ CEC ++ C + PVCG+DK TY + C L TAC KK + I++ G C+ RC + C IC+++ +C CPT C S + VCGTDG TY+SEC + + AC T I L++A G C C N C+F + C N QC+C T PVCGS+ TY+N+C LK SC + +LI G C+ + ++ C +D CR + +CVC C V ++ VCG+DG Y NECELK+ C + + VA +V GC N A SC C+ GS CDP+T QC CK G+ G CDRC G+WNF ++GCT C+C+ G+ C TG C+CK G CS CP G GC+++ DA++ F
Sbjct: 42 ANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNK---VMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACAS--IVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDP------------CSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRV------------NPR---TRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQ---DKCKE-DCKHNSVCLNRRG-VTRCSCDRVTCDSTYK-PVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCS--FGAACVVKNKKAVCEC-QQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCD---RCGK----CQFGAICEAET--GRCVCPTECVPSA--QPVCGTDGNTYESECELHVRAC-TQQINLQVAAQGDCKTCGNTLCTFGAKCVNN-----QCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQD-----RCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQF 913 HSP 2 Score: 160.229 bits (404), Expect = 1.497e-38 Identity = 179/753 (23.77%), Postives = 307/753 (40.77%), Query Frame = 1
Query: 2725 FLKDSFAKSYWSGNA-ENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITC---------------------------------------EEVVSHIWKF----KDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKI------LINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE-NLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKR-----------DKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLI--DDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGF--PWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ KSY + + Y ++ I++ SS+ G++ Y+ + D++++ + N +VE R + GSG + S+V + G+ H ++V R R L +DG G +P L+ IG A + +S ++ G GC+ L + ++Y + + + ++ S V E N + CQ + PC NG CQ EM+ CQCL GY G TC E+V + + N + + LQ L+ +L +E+ FL NGL+ + D F+S++L+ +LE G + S E + W V + ++ + + +N+ Q K R P LN+ +P+F GG + + + S +F GAIQKI ++ +ENIR S I I+ ++ PC PC G C P C C + C + +++ A + + GK+ +Y KR +K+ N + KT + G+ IL + + ++ +A+ G +E+ +LG K VLR T ++ N+W ++ +R+NR+ +L + +D + + + L TDG +WLGG +K + P Y+T F GC+ ++ VD+ +LV DA N PIL C
Sbjct: 1411 FIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQS-LGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVN----NQLYNLQEGGADVLYGSGV------------------------GECGN----NPCQPN-PCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNL--------------KEPLFVGGAPDFSKLARAAAAST--SFEGAIQKISIKGLPVLREENIR---SAI---EISTFRSHPCTKKPFPCQN----GGLCSPNKGNYECVCQRGFSGSQCEKVTIE--KAAGESEAVSFDGKTYLEYHIAVTKRTGPVDNPDDPSEKALLVNQFELSIKTEATQGL-ILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRST--VPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKE---LDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDAL-NKPPILYC 2094 HSP 3 Score: 117.857 bits (294), Expect = 1.323e-25 Identity = 154/568 (27.11%), Postives = 215/568 (37.85%), Query Frame = 1
Query: 526 VQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK----------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE----NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPRCKEQGK---DCPSYQICKSDLVQAK--CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ-MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ + PC C FGA C AVC C CQA VCGSD Y + C ++ C L+K I + C R Q G C+ ++ C CP + C S+ +CGTD TY + CE+ +C ++ G C C C F C + C C C + +PVCGS+ VTY ++C LK SCK+ + V G C+ + + C +C +C CD+ C R VCG+DGI Y N+C L K CEK+ +++V C ++P C C DN + CP Q + +Y C+ T C K +P + + GI + K+ G +C + D Q C C T SGL S C DG+ + CE + A K C KC F + C + F +C P VCGSDD TY + C LK +C + R IT GPC+
Sbjct: 468 LHSPSPCLNTTCSFGAACVV---KNKKAVCECQQV-CQAVYD--PVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGA------ICEAET------------GRCVCPTE-CVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKC------VNNQCVCLT----CERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFT-CHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNI-TAALGVGLAGCPSSCQ---CHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTD----KKSGCTPCNCDPVGAVRDDCEQMTGLCSCKT-GISGLKCSQC-PDGR------KPGLTGCEKDPSAPK--------SCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQ 975 HSP 4 Score: 70.0922 bits (170), Expect = 4.437e-11 Identity = 88/435 (20.23%), Postives = 161/435 (37.01%), Query Frame = 1
Query: 2560 GSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVD--AVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFL----TNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIF----------------PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILK 3750
G C E C+ P + C V P G + + KV + L FL SY ++ + ++ L++ + + + +G+IFY+ TD D++++ L + +EYR DLG GA +KS+ ++ + S+ +ER R + I+ + G +P T L+ +G A P + L+ + F G + + I + V+ + + E + + PC NGGLC + ++ Y C C G+ G CE+V S F +++ + PSE L + +L I A GL+ + G +R +I++ + ++E+T L S +W H+ ++
Sbjct: 1617 GYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGA--PDFSKLARAAAASTSFEGAIQKISI----------------------------------KGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLC-SPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKRTGPVDNPDDPSEKALLVNQFELSIKT--EATQGLILWSGKGTERA-YYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVR 2011 HSP 5 Score: 68.9366 bits (167), Expect = 8.557e-11 Identity = 88/437 (20.14%), Postives = 175/437 (40.05%), Query Frame = 1
Query: 3355 CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDE--NIRFQNSDIILNN---------------------------------ITQWQGPPCGPLQSPCSKTDP------EKGFCVPEMNETICTCSPKHEN-LCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
C++H PCL+GG C+ + ++ ++C C G G CE+ + + I F S++ F + ++ +++ + F NGLL + +G FIS+ +N +E+ T G + S GKW+ +V+ + + L+++ G +VN + +F GG + S SL G I+++ +N++ N++ +D++ + ++ + GP C ++PC DP +PE C C E C + S Q N F ++ G S + ++ K + + F ++ +G++ + K F+ +++ +G +E +LG G I + I N W +V + R+ R ++ I++
Sbjct: 1373 CESH-PCLHGGTCEDNGKD-FTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAF--KMMKAYHTVRIAMEFRSSEQNGLLLY--NGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVD-------------GESHVNGESPSGTDGLNLDTD-LFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPC---DPNPCHVSATCLVLPEGGAK-CECPMGREGEFCEKVSNQD---LNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLG--KGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINN 1780 HSP 6 Score: 56.9954 bits (136), Expect = 4.010e-7 Identity = 30/77 (38.96%), Postives = 44/77 (57.14%), Query Frame = 1
Query: 1021 CKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKI 1251
C +KC+F C ++ G C+CP+P LC + VCGSDDVTY C LK +C++ ++TV + G CQ +
Sbjct: 906 CDAIKCQFGATCVVI--GGFAHCECPSP--LCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETV 978
BLAST of Agrin, putative vs. Ensembl Xenopus
Match: sos1 (SOS Ras/Rac guanine nucleotide exchange factor 1 [Source:Xenbase;Acc:XB-GENE-1011399]) HSP 1 Score: 307.76 bits (787), Expect = 6.413e-84 Identity = 270/935 (28.88%), Postives = 407/935 (43.53%), Query Frame = 1
Query: 202 SDLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFE----------------KRISVFLNGAT-SKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQV------------PVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE---NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQ-------FIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVAP--------------------SVIGCISHNYIAD-----EKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGF 2727
+++++ G V + N + + V V R LKG +++ + N ++V C + +G+ I ++ ++ + + L +R+S G + Q D C + C FGA C+K +P A C C T C + VCGSD Y + C ++R +C Q+ I+V CG K+ C SC +TCV + C CP + +CG+D Y N C++ K +C + +++ GRCD C+ N C + NP T+ +++ PVC D VTY ++C + + I L ++ G+CQ + D C C + + C+ V +CSCD + C +T++ PVCG D Y N C K C ++ + V + C+ +PC C + + N K+ CEC ++ C + PVCG+DK TY + C L TAC KK + I++ G C+ RC + C IC+++ +C CPT C S + VCGTDG TY+SEC + + AC T I L++A G C C N C+F + C N QC+C T PVCGS+ TY+N+C LK SC + +LI G C+ + ++ C +D CR + +CVC C V ++ VCG+DG Y NECELK+ C + + VA +V GC N A SC C+ GS CDP+T QC CK G+ G CDRC G+WNF ++GCT C+C+ G+ C TG C+CK G CS CP G GC+++ DA++ F
Sbjct: 42 ANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNK---VMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACAS--IVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDP------------CSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRV------------NPR---TRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQ---DKCKE-DCKHNSVCLNRRG-VTRCSCDRVTCDSTYK-PVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCS--FGAACVVKNKKAVCEC-QQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCD---RCGK----CQFGAICEAET--GRCVCPTECVPSA--QPVCGTDGNTYESECELHVRAC-TQQINLQVAAQGDCKTCGNTLCTFGAKCVNN-----QCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQD-----RCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQF 913 HSP 2 Score: 166.392 bits (420), Expect = 1.953e-40 Identity = 178/742 (23.99%), Postives = 306/742 (41.24%), Query Frame = 1
Query: 2725 FLKDSFAKSYWSGNA-ENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITC---------------------------------------EEVVSHIWKF----KDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKI------LINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE-NLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLI--DDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGF--PWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ KSY + + Y ++ I++ SS+ G++ Y+ + D++++ + N +VE R + GSG + S+V + G+ H ++V R R L +DG G +P L+ IG A + +S ++ G GC+ L + ++Y + + + ++ S V E N + CQ + PC NG CQ EM+ CQCL GY G TC E+V + + N + + LQ L+ +L +E+ FL NGL+ + D F+S++L+ +LE G + S E + W V + ++ + + +N+ Q K R P LN+ +P+F GG + + + S +F GAIQKI ++ +ENIR S I I+ ++ PC PC G C P C C + C + +++ A + + GK+ +Y K +K+ N + KT + G+ IL + + ++ +A+ G +E+ +LG K VLR T ++ N+W ++ +R+NR+ +L + +D + + + L TDG +WLGG +K + P Y+T F GC+ ++ VD+ +LV DA N PIL C
Sbjct: 1411 FIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQS-LGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVN----NQLYNLQEGGADVLYGSGV------------------------GECGN----NPCQPN-PCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNL--------------KEPLFVGGAPDFSKLARAAAAST--SFEGAIQKISIKGLPVLREENIR---SAI---EISTFRSHPCTKKPFPCQN----GGLCSPNKGNYECVCQRGFSGSQCEKVTIE--KAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGL-ILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRST--VPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKE---LDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDAL-NKPPILYC 2083 HSP 3 Score: 117.087 bits (292), Expect = 1.882e-25 Identity = 154/568 (27.11%), Postives = 215/568 (37.85%), Query Frame = 1
Query: 526 VQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK----------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE----NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPRCKEQGK---DCPSYQICKSDLVQAK--CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ-MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ + PC C FGA C AVC C CQA VCGSD Y + C ++ C L+K I + C R Q G C+ ++ C CP + C S+ +CGTD TY + CE+ +C ++ G C C C F C + C C C + +PVCGS+ VTY ++C LK SCK+ + V G C+ + + C +C +C CD+ C R VCG+DGI Y N+C L K CEK+ +++V C ++P C C DN + CP Q + +Y C+ T C K +P + + GI + K+ G +C + D Q C C T SGL S C DG+ + CE + A K C KC F + C + F +C P VCGSDD TY + C LK +C + R IT GPC+
Sbjct: 468 LHSPSPCLNTTCSFGAACVV---KNKKAVCECQQV-CQAVYD--PVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGA------ICEAET------------GRCVCPTE-CVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKC------VNNQCVCLT----CERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFT-CHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNI-TAALGVGLAGCPSSCQ---CHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTD----KKSGCTPCNCDPVGAVRDDCEQMTGLCSCKT-GISGLKCSQC-PDGR------KPGLTGCEKDPSAPK--------SCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQ 975 HSP 4 Score: 71.633 bits (174), Expect = 1.243e-11 Identity = 84/422 (19.91%), Postives = 160/422 (37.91%), Query Frame = 1
Query: 2560 GSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVD--AVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFL----TNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIFPSESLQLRQRL---KLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILK 3750
G C E C+ P + C V P G + + KV + L FL SY ++ + ++ L++ + + + +G+IFY+ TD D++++ L + +EYR DLG GA +KS+ ++ + S+ +ER R + I+ + G +P T L+ +G A P + L+ + F G + + I + V+ + + E + + PC NGGLC + ++ Y C C G+ G CE+V S F +++ + + + L + E++ A GL+ + G +R +I++ + ++E+T L S +W H+ ++
Sbjct: 1617 GYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGA--PDFSKLARAAAASTSFEGAIQKISI----------------------------------KGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLC-SPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGLILWSGKGTERA-YYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVR 2000 HSP 5 Score: 68.9366 bits (167), Expect = 8.479e-11 Identity = 88/437 (20.14%), Postives = 175/437 (40.05%), Query Frame = 1
Query: 3355 CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDE--NIRFQNSDIILNN---------------------------------ITQWQGPPCGPLQSPCSKTDP------EKGFCVPEMNETICTCSPKHEN-LCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
C++H PCL+GG C+ + ++ ++C C G G CE+ + + I F S++ F + ++ +++ + F NGLL + +G FIS+ +N +E+ T G + S GKW+ +V+ + + L+++ G +VN + +F GG + S SL G I+++ +N++ N++ +D++ + ++ + GP C ++PC DP +PE C C E C + S Q N F ++ G S + ++ K + + F ++ +G++ + K F+ +++ +G +E +LG G I + I N W +V + R+ R ++ I++
Sbjct: 1373 CESH-PCLHGGTCEDNGKD-FTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAF--KMMKAYHTVRIAMEFRSSEQNGLLLY--NGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVD-------------GESHVNGESPSGTDGLNLDTD-LFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPC---DPNPCHVSATCLVLPEGGAK-CECPMGREGEFCEKVSNQD---LNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLG--KGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINN 1780 HSP 6 Score: 58.5362 bits (140), Expect = 1.173e-7 Identity = 53/219 (24.20%), Postives = 96/219 (43.84%), Query Frame = 1
Query: 2623 GKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWS-GNAENYLSIN---ISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDV----TDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP 3255
G Y VC G+S S C++ +I + ++ + F ++ + + + +E L +N +S+ ++ G+I + + Y+ + + YVE DLGS + L+S V + I R NRD L + D +G +P + LD +G I LS + + + GF+GC+ K L++QQ ++E + KP
Sbjct: 1878 GNYECVCQRGFSGSQ--CEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGG-----IEKLSASYKLPKAYSTGFIGCM-----------KDVLVDQQDLHLVEDALNKP 2078 HSP 7 Score: 56.6102 bits (135), Expect = 4.323e-7 Identity = 30/77 (38.96%), Postives = 44/77 (57.14%), Query Frame = 1
Query: 1021 CKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKI 1251
C +KC+F C ++ G C+CP+P LC + VCGSDDVTY C LK +C++ ++TV + G CQ +
Sbjct: 906 CDAIKCQFGATCVVI--GGFAHCECPSP--LCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETV 978
BLAST of Agrin, putative vs. Ensembl Xenopus
Match: sos1 (SOS Ras/Rac guanine nucleotide exchange factor 1 [Source:Xenbase;Acc:XB-GENE-1011399]) HSP 1 Score: 307.76 bits (787), Expect = 6.481e-84 Identity = 266/918 (28.98%), Postives = 402/918 (43.79%), Query Frame = 1
Query: 202 SDLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQV------------PVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE---NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQ-------FIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVAP--------------------SVIGCISHNYIAD-----EKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGF 2727
+++++ G V + N + + V V R LKG +++ + N ++V C + +G+ I ++ ++ + + L + +V C + C FGA C+K +P A C C T C + VCGSD Y + C ++R +C Q+ I+V CG K+ C SC +TCV + C CP + +CG+D Y N C++ K +C + +++ GRCD C+ N C + NP T+ +++ PVC D VTY ++C + + I L ++ G+CQ + D C C + + C+ V +CSCD + C +T++ PVCG D Y N C K C ++ + V + C+ +PC C + + N K+ CEC ++ C + PVCG+DK TY + C L TAC KK + I++ G C+ RC + C IC+++ +C CPT C S + VCGTDG TY+SEC + + AC T I L++A G C C N C+F + C N QC+C T PVCGS+ TY+N+C LK SC + +LI G C+ + ++ C +D CR + +CVC C V ++ VCG+DG Y NECELK+ C + + VA +V GC N A SC C+ GS CDP+T QC CK G+ G CDRC G+WNF ++GCT C+C+ G+ C TG C+CK G CS CP G GC+++ DA++ F
Sbjct: 42 ANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNK---VMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEV-----CRGMLCGFGAVCEKSSTDPSQATCICKKTACAS--IVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDP------------CSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRV------------NPR---TRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQ---DKCKE-DCKHNSVCLNRRG-VTRCSCDRVTCDSTYK-PVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCS--FGAACVVKNKKAVCEC-QQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCD---RCGK----CQFGAICEAET--GRCVCPTECVPSA--QPVCGTDGNTYESECELHVRAC-TQQINLQVAAQGDCKTCGNTLCTFGAKCVNN-----QCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQD-----RCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQF 891 HSP 2 Score: 157.147 bits (396), Expect = 1.112e-37 Identity = 172/743 (23.15%), Postives = 299/743 (40.24%), Query Frame = 1
Query: 2725 FLKDSFAKSYWSGNA-ENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEE----------------------------VVSHIWKF------KDNSFVIFPS-------ESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKI------LINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE-NLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTK------------NNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLI--DDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGF--PWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ KSY + + Y ++ I++ SS+ G++ Y+ + D++++ + N +VE R + GSG + S+V + G+ H ++V R R L +DG G +P L+ IG A + +S ++ G GC+ L + ++Y + + + ++ S V + +P CQ + PC NG CQ EM+ CQCL GY G TC + + +F +D + P E L+ L +E+ FL NGL+ + D F+S++L+ +LE G + S E + W V + ++ + + +N+ Q + P LN+ +P+F GG + + + S +F GAIQKI ++ +ENIR S I I+ ++ PC PC G C P C C + C + +++ A + + GK+ +Y K + + N + KT + G+++ K +A+ G +E+ +LG K VLR T ++ N+W ++ +R+NR+ +L + +D + + + L TDG +WLGG +K + P Y+T F GC+ ++ VD+ +LV DA N PIL C
Sbjct: 1362 FIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQS-LGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVN----NQLYNLQEGGADVLYGSGVG------------ECGNNP----------------CQPN-PCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPAPHTALNLK--------------EPLFVGGAPDFSKLARAAAAST--SFEGAIQKISIKGLPVLREENIR---SAI---EISTFRSHPCTKKPFPCQN----GGLCSPNKGNYECVCQRGFSGSQCEKVTIE--KAAGESEAVSFDGKTYLEYHIAVTKSTQIPSPFIHFLGSLALLVNQFELSIKTEATQGLILWSGKGTERAYYI-ALAVVGGYVEMTYDLGSKPVVLRST--VPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKE---LDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDAL-NKPPILYC 2035 HSP 3 Score: 117.857 bits (294), Expect = 1.052e-25 Identity = 154/568 (27.11%), Postives = 215/568 (37.85%), Query Frame = 1
Query: 526 VQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK----------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE----NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPRCKEQGK---DCPSYQICKSDLVQAK--CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ-MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ + PC C FGA C AVC C CQA VCGSD Y + C ++ C L+K I + C R Q G C+ ++ C CP + C S+ +CGTD TY + CE+ +C ++ G C C C F C + C C C + +PVCGS+ VTY ++C LK SCK+ + V G C+ + + C +C +C CD+ C R VCG+DGI Y N+C L K CEK+ +++V C ++P C C DN + CP Q + +Y C+ T C K +P + + GI + K+ G +C + D Q C C T SGL S C DG+ + CE + A K C KC F + C + F +C P VCGSDD TY + C LK +C + R IT GPC+
Sbjct: 446 LHSPSPCLNTTCSFGAACVV---KNKKAVCECQQV-CQAVYD--PVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGA------ICEAET------------GRCVCPTE-CVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKC------VNNQCVCLT----CERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFT-CHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNI-TAALGVGLAGCPSSCQ---CHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTD----KKSGCTPCNCDPVGAVRDDCEQMTGLCSCKT-GISGLKCSQC-PDGR------KPGLTGCEKDPSAPK--------SCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQ 953 HSP 4 Score: 77.7962 bits (190), Expect = 1.645e-13 Identity = 87/423 (20.57%), Postives = 158/423 (37.35%), Query Frame = 1
Query: 2560 GSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVD--AVELGFLKDSFAKSYWSGNA-ENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIF----------PSESLQLRQRLKL-----EITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILK 3750
G C E C+ P + C V P G + + KV + L FL SY + +L++ + + + +G+IFY+ TD D++++ L + +EYR DLG GA +KS+ ++ + S+ +ER R + I+ + G +P T L+ +G A P + L+ + F G + + I + V+ + + E + + PC NGGLC + ++ Y C C G+ G CE+V S F +++ + PS + L L E++ A GL+ + G +R +I++ + ++E+T L S +W H+ ++
Sbjct: 1568 GYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPAPHTALNLKEPLFVGGA--PDFSKLARAAAASTSFEGAIQKISI----------------------------------KGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLC-SPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSTQIPSPFIHFLGSLALLVNQFELSIKTEATQGLILWSGKGTER-AYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVR 1952 HSP 5 Score: 67.0106 bits (162), Expect = 3.142e-10 Identity = 97/489 (19.84%), Postives = 195/489 (39.88%), Query Frame = 1
Query: 3355 CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDE--NIRFQNSDIILNN---------------------------------ITQWQGPPCGPLQSPCSKTDP------EKGFCVPEMNETICTCSPKHEN-LCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVE-GFPWEYNTKFKGCISELYV 4689
C++H PCL+GG C+ + ++ ++C C G G CE+ + + I F S++ F + ++ +++ + F NGLL + +G FIS+ +N +E+ T G + S GKW+ +V+ + + L+++ G +VN + +F GG + S SL G I+++ +N++ N++ +D++ + ++ + GP C ++PC DP +PE C C E C + S Q N F ++ G S + ++ T + + F ++ +G++ + K F+ +++ +G +E +LG G I + I N W +V + R+ R ++ I++ M + + L +++GGA + +T F+G I ++ +
Sbjct: 1324 CESH-PCLHGGTCEDNGKD-FTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAF--KMMKAYHTVRIAMEFRSSEQNGLLLY--NGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVD-------------GESHVNGESPSGTDGLNLDTD-LFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPC---DPNPCHVSATCLVLPEGGAK-CECPMGREGEFCEKVSNQD---LNLPFLPQFNGYSYLEMKGLQ-------TFLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLG--KGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINN-MEQVTGESPAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISI 1775 HSP 6 Score: 56.9954 bits (136), Expect = 3.963e-7 Identity = 30/77 (38.96%), Postives = 44/77 (57.14%), Query Frame = 1
Query: 1021 CKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKI 1251
C +KC+F C ++ G C+CP+P LC + VCGSDDVTY C LK +C++ ++TV + G CQ +
Sbjct: 884 CDAIKCQFGATCVVI--GGFAHCECPSP--LCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETV 956
BLAST of Agrin, putative vs. Ensembl Xenopus
Match: sos1 (SOS Ras/Rac guanine nucleotide exchange factor 1 [Source:Xenbase;Acc:XB-GENE-1011399]) HSP 1 Score: 307.76 bits (787), Expect = 6.709e-84 Identity = 271/926 (29.27%), Postives = 410/926 (44.28%), Query Frame = 1
Query: 202 SDLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQN------NFFELNYA--KEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQV------------PVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE---NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQ-------FIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVAP--------------------SVIGCISHNYIAD-----EKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGF 2727
+++++ G V + N + + V V R LKG +++ + N ++V C + +G+ I ++ ++ N LN + + L E + V N + + D+ C + C FGA C+K +P A C C T C + VCGSD Y + C ++R +C Q+ I+V CG++ C SC +TCV + C CP + +CG+D Y N C++ K +C + +++ GRCD C+ N C + NP T+ +++ PVC D VTY ++C + + I L ++ G+CQ + D C C + + C+ V +CSCD + C +T++ PVCG D Y N C K C ++ + V + C+ +PC C + + N K+ CEC ++ C + PVCG+DK TY + C L TAC KK + I++ G C+ RC + C IC+++ +C CPT C S + VCGTDG TY+SEC + + AC T I L++A G C C N C+F + C N QC+C T PVCGS+ TY+N+C LK SC + +LI G C+ + ++ C +D CR + +CVC C V ++ VCG+DG Y NECELK+ C + + VA +V GC N A SC C+ GS CDP+T QC CK G+ G CDRC G+WNF ++GCT C+C+ G+ C TG C+CK G CS CP G GC+++ DA++ F
Sbjct: 42 ANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNK---VMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEGNFMFIQFMPIDNMCRGMLCGFGAVCEKSSTDPSQATCICKKTACAS--IVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGKRCDP-----------CSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRV------------NPR---TRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQ---DKCKE-DCKHNSVCLNRRG-VTRCSCDRVTCDSTYK-PVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCS--FGAACVVKNKKAVCEC-QQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCD---RCGK----CQFGAICEAET--GRCVCPTECVPSA--QPVCGTDGNTYESECELHVRAC-TQQINLQVAAQGDCKTCGNTLCTFGAKCVNN-----QCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQD-----RCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQF 905 HSP 2 Score: 165.622 bits (418), Expect = 3.283e-40 Identity = 169/732 (23.09%), Postives = 298/732 (40.71%), Query Frame = 1
Query: 2725 FLKDSFAKSYWSGNA-ENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITC---------------------------------------EEVVSHIWKF----KDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE-NLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLI--DDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGF--PWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ KSY + + Y ++ I++ SS+ G++ Y+ + D++++ + N +VE R + GSG + S+V + G+ H ++V R R L +DG G +P L+ IG A + +S ++ G GC+ L + ++Y + + + ++ S V + +P CQ + PC NG CQ EM+ CQCL GY G TC E+V + + N + + LQ L+ +L +E+ FL NGL+ + D F+S++L+ +LE G + S E + W V + ++ + + +N+ Q + P LN+ +P+F GG + + + S +F GAIQK+ + N I+ ++ PC PC G C P C C + C + +++ A + + GK+ +Y K +K+ N + KT + G+ IL + + ++ +A+ G +E+ +LG K VLR T ++ N+W ++ +R+NR+ +L + +D + + + L TDG +WLGG +K + P Y+T F GC+ ++ VD+ +LV DA N PIL C
Sbjct: 1378 FIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQS-LGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVN----NQLYNLQEGGADVLYGSGVG------------ECGNNP----------------CQPN-PCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPAPHTALNL--------------KEPLFVGGAPDFSKLARAAAAST--SFEGAIQKV-----------GSLFRNVISTFRSHPCTKKPFPCQN----GGLCSPNKGNYECVCQRGFSGSQCEKVTIE--KAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGL-ILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRST--VPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKE---LDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDAL-NKPPILYC 2035 HSP 3 Score: 117.857 bits (294), Expect = 1.035e-25 Identity = 154/568 (27.11%), Postives = 215/568 (37.85%), Query Frame = 1
Query: 526 VQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK----------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE----NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPRCKEQGK---DCPSYQICKSDLVQAK--CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ-MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
+ + PC C FGA C AVC C CQA VCGSD Y + C ++ C L+K I + C R Q G C+ ++ C CP + C S+ +CGTD TY + CE+ +C ++ G C C C F C + C C C + +PVCGS+ VTY ++C LK SCK+ + V G C+ + + C +C +C CD+ C R VCG+DGI Y N+C L K CEK+ +++V C ++P C C DN + CP Q + +Y C+ T C K +P + + GI + K+ G +C + D Q C C T SGL S C DG+ + CE + A K C KC F + C + F +C P VCGSDD TY + C LK +C + R IT GPC+
Sbjct: 460 LHSPSPCLNTTCSFGAACVV---KNKKAVCECQQV-CQAVYD--PVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGA------ICEAET------------GRCVCPTE-CVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKC------VNNQCVCLT----CERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFT-CHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNI-TAALGVGLAGCPSSCQ---CHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTD----KKSGCTPCNCDPVGAVRDDCEQMTGLCSCKT-GISGLKCSQC-PDGR------KPGLTGCEKDPSAPK--------SCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQ 967 HSP 4 Score: 70.8626 bits (172), Expect = 2.329e-11 Identity = 84/418 (20.10%), Postives = 158/418 (37.80%), Query Frame = 1
Query: 2560 GSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVD--AVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIFPSESLQLRQRL---KLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILK 3750
G C E C+ P + C V P G + + KV + L FL SY ++ + ++ L++ + + + +G+IFY+ TD D++++ L + +EYR DLG GA +KS+ ++ + S+ +ER R + I+ + G +P T L+ +G A P + L+ + F G + + F+N + + K+ PC NGGLC + ++ Y C C G+ G CE+V S F +++ + + + L + E++ A GL+ + G +R +I++ + ++E+T L S +W H+ ++
Sbjct: 1584 GYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPAPHTALNLKEPLFVGGA--PDFSKLARAAAASTSFEGAIQKVGSL-------------------------------FRNVISTF----RSHPCTKKPF----------PCQNGGLC-SPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGLILWSGKGTERA-YYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVR 1952 HSP 5 Score: 70.4774 bits (171), Expect = 2.733e-11 Identity = 93/464 (20.04%), Postives = 185/464 (39.87%), Query Frame = 1
Query: 3355 CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDE--NIRFQNSDIILNN---------------------------------ITQWQGPPCGPLQSPCSKTDP------EKGFCVPEMNETICTCSPKHEN-LCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGA 4617
C++H PCL+GG C+ + ++ ++C C G G CE+ + + I F S++ F + ++ +++ + F NGLL + +G FIS+ +N +E+ T G + S GKW+ +V+ + + L+++ G +VN + +F GG + S SL G I+++ +N++ N++ +D++ + ++ + GP C ++PC DP +PE C C E C + S Q N F ++ G S + ++ K + + F ++ +G++ + K F+ +++ +G +E +LG G I + I N W +V + R+ R ++ I++ M + + L +++GGA
Sbjct: 1340 CESH-PCLHGGTCEDNGKD-FTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAF--KMMKAYHTVRIAMEFRSSEQNGLLLY--NGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVD-------------GESHVNGESPSGTDGLNLDTD-LFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPC---DPNPCHVSATCLVLPEGGAK-CECPMGREGEFCEKVSNQD---LNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLG--KGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINN-MEQVTGESPAPHTALNLKEPLFVGGA 1773 HSP 6 Score: 58.5362 bits (140), Expect = 1.140e-7 Identity = 53/219 (24.20%), Postives = 96/219 (43.84%), Query Frame = 1
Query: 2623 GKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWS-GNAENYLSIN---ISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDV----TDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP 3255
G Y VC G+S S C++ +I + ++ + F ++ + + + +E L +N +S+ ++ G+I + + Y+ + + YVE DLGS + L+S V + I R NRD L + D +G +P + LD +G I LS + + + GF+GC+ K L++QQ ++E + KP
Sbjct: 1830 GNYECVCQRGFSGSQ--CEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGG-----IEKLSASYKLPKAYSTGFIGCM-----------KDVLVDQQDLHLVEDALNKP 2030 HSP 7 Score: 56.9954 bits (136), Expect = 4.133e-7 Identity = 30/77 (38.96%), Postives = 44/77 (57.14%), Query Frame = 1
Query: 1021 CKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKI 1251
C +KC+F C ++ G C+CP+P LC + VCGSDDVTY C LK +C++ ++TV + G CQ +
Sbjct: 898 CDAIKCQFGATCVVI--GGFAHCECPSP--LCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETV 970
BLAST of Agrin, putative vs. Ensembl Mouse
Match: Agrn (agrin [Source:MGI Symbol;Acc:MGI:87961]) HSP 1 Score: 315.079 bits (806), Expect = 2.401e-86 Identity = 255/907 (28.11%), Postives = 393/907 (43.33%), Query Frame = 1
Query: 205 DLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKT------EQNNFFELNYAK-----------EFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKN----IQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGK-MCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-------PSVI---------------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFT------ENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
++++ G V + N + + V V R LKG D++ + + N +++ + C + +G+ I ++ N LN + EF E + + A S D C + C FGA C+ +P A C C C A VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TCV + C CP + +CG+D + Y + C++ + +C I +++ G CD C+ + N IC + + +P+ L P+CG D VTY+++C + + + L + G+CQT+ D C C + + C+ CSCD + C +R PVC DG YDN C+ + C +Q + + C+ +PC G +C + NGK+ CEC + C PVCG+D VTY S+C L AC + + + G C+ RC + C +C+ + +C CP+ C +S + VCG+DG TY SEC + +HAC T+ I+L +A+ G C C C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED + +CR +G CVC C V K VCG+DG Y EC LK+ C + + VA P++ GC N A + +C CN GS + CDP T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+C+P G C CP G ++ + GC+ +
Sbjct: 46 NVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNK---VVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEF---CVEDKPGIHFTAAPS---MPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPA--MVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDP------------CANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNP-------RTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTR---DQCPET-CQFNSVCLSRRGR-PHCSCDRVTCDGAYR-PVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCA--FGATCTVKNGKAVCEC-QRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCD---RCGQ----CRFGSLCEVE--TGRCVCPSECVESA--QPVCGSDGHTYASECELHVHAC-THQISLYVASAGHCQTCGETVCTFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEAD 896 HSP 2 Score: 160.229 bits (404), Expect = 1.078e-38 Identity = 162/718 (22.56%), Postives = 293/718 (40.81%), Query Frame = 1
Query: 2650 GYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV--------KPNCPHID-----FKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCG-PLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G+S S + ++ E KV + K ++ + A + L + + ++G++ Y+ + R D+L + L + +V++R D GSG L S V + GR H + + R R L +DG G +P L+ +G + T+ V G GC+ L I ++ L + Q + +++SS V PN H + V + + P + K + CQ + PC C S C C G G CE V+ S + N F + L L +++ LE+ FL +GLL + D F+S+ L+ HLE G + S E +G W V + + + L + P+ + + +P LN+ +P++ GG F+ + ++ F GAIQ + + + Q + ++ + G PC + +PC G C+P C C L + + + + G++ +Y + +K+ N+ + +T + G+ +L + ++ +AI +G ++++ +LG + VLR T ++ N+W V+ R +R L + + A + ++ L TDG +WLGG +K PV + P Y T F GC+ ++ V +L+ DA
Sbjct: 1341 GFSCSCTAGRAGTVCE-KVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNA-RGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDIN---NQQLELSDWQRA-VVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEK--NPCQPN-PCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNL--------------KEPLYVGGAP--DFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLN----GGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETL-AFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGL-VLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRST--VKVNTNRWLRVRAHREHREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDA 2022 HSP 3 Score: 138.658 bits (348), Expect = 4.640e-32 Identity = 150/586 (25.60%), Postives = 217/586 (37.03%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGK---------STCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGI----------CNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCG--TDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC C FGA C G AVC C C G VCGSDG+ Y S C ++ C L + I+V+ C R + G C+ ++ C CP++ C S+ +CG+D TY + CE+ +C + G C C C F +C + C CP C PVCGSD VTY S C L+ +C++++ + G C+ + + C C + C CD+ C++ + PVCG+DG+ Y +C L K CE + E++V C P + C PY C+ + STC C N +Y C+ C + +P + + GI C+ +PR + DC + + C C + G+ CG DG+ H+ A T + C C F + C QC+CPT C VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 465 QTPSPCRGAQCAFGATCTVKNGK---AVCECQRV-CSGGYD--PVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFG------SLCEVET------------GRCVCPSE-CVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDF-SCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHC--------------NPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVR-DDC--------EQMTGLCSC----RPGVAGPKCGQCPDGQALG---HLGCEADPTTPVT-----------CVEMHCEFGASCVEEAGFA-QCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCR 972 HSP 4 Score: 85.5001 bits (210), Expect = 5.917e-16 Identity = 84/377 (22.28%), Postives = 152/377 (40.32%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-------HIWKFKDNSFVIF---PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVT 3666
G C L+ G CP+G S G ++ EN A FL D SY + + +++ + + G++ Y+ TD D++++ L NR++E+R DLG GA ++S+ + +G + +ER R L + G +P T L+ +G A P + L+ V GF G + + + + L+ Q+ ++ + +V P H +VD PCLNGG C E Y C C G+ G+ CE+ + F +++ + +ES + Q E++ A GL+ +I +R ++++ + HL+++
Sbjct: 1588 GSAPCHVLSRGGAKCACPLGRS----GSFCETVLEN---AGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGA--PDFSKLARGAAVASGFDGAIQLVSLRGHQ-----LLTQE--HVLRAVDVAPFAGH-PCTQAVD-------------------------NPCLNGGSC-IPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERA-DYMALAIVDGHLQLS 1920 HSP 5 Score: 73.9442 bits (180), Expect = 1.924e-12 Identity = 88/432 (20.37%), Postives = 167/432 (38.66%), Query Frame = 1
Query: 3367 KPCLNGGLCQ-TSSEEMYSCQCLPGYQGITCEEV-VSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIR---FQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPK-------------HENLCIRNSMQH------------------------DNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PCL+GG CQ S + +SC C G G CE+V + + FK +SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L H++ T G L S+ G+W+ + + + + L+++ + + LN+++ ++ GG+ + + S+ G I+ + IN++ + +Q + + + + + PC P +PC + E +C C P N C ++ H +N + F + G S + + K + + F G+L+ + K F+ +A+ N +E +LG ++R + I+ W V + RN R L + D
Sbjct: 1325 QPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFP--TLRAYHTLRLALEFRALETEGLLLY--NGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTK--------------LYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSP--NPCHGGALCQAL---EAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIR--SKEPIALGTWVRVFLERNGRKGALQVGD 1731 HSP 6 Score: 59.3066 bits (142), Expect = 4.993e-8 Identity = 65/288 (22.57%), Postives = 110/288 (38.19%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTN-----PYPYCKCPND-KCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIES--CKKKISLTVMYKGKCQTKIQSDI-CSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGV 1497
VCGSDG+ Y + C +K+ C ++ + V+ C R + ++ Q PY C N CP+ C+ S Y+ C+ C + RCD C+ F GI G T C C +P + + +T C + C + + C+ + + C + C +GA C++ + AQC C L C VCG+DG+ Y N+C L ++C +++++ + + C + GV
Sbjct: 719 VCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-RGPTLAPLLPMASPHCAQT----PYGCCQDNVTAAQGVGLAGCPSTCHCNPHGS---------YSGTCDPVTGQCSCRPGVGGL---RCDRCEPGFWNFRGIVTDGHSGC-TPCSC-DPRGAVRDDCE-------QMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAG-FAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGV 979
BLAST of Agrin, putative vs. Ensembl Mouse
Match: Agrn (agrin [Source:MGI Symbol;Acc:MGI:87961]) HSP 1 Score: 310.457 bits (794), Expect = 6.037e-85 Identity = 234/780 (30.00%), Postives = 350/780 (44.87%), Query Frame = 1
Query: 514 TSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKN----IQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGK-MCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-------PSVI---------------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFT------ENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
T+ D C + C FGA C+ +P A C C C A VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TCV + C CP + +CG+D + Y + C++ + +C I +++ G CD C+ + N IC + + +P+ L P+CG D VTY+++C + + + L + G+CQT+ D C C + + C+ CSCD + C +R PVC DG YDN C+ + C +Q + + C+ +PC G +C + NGK+ CEC + C PVCG+D VTY S+C L AC + + + G C+ RC + C +C+ + +C CP+ C +S + VCG+DG TY SEC + +HAC T+ I+L +A+ G C C C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED + +CR +G CVC C V K VCG+DG Y EC LK+ C + + VA P++ GC N A + +C CN GS + CDP T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+C+P G C CP G ++ + GC+ +
Sbjct: 57 TAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPA--MVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDP------------CANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNP-------RTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTR---DQCPET-CQFNSVCLSRRGR-PHCSCDRVTCDGAYR-PVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCA--FGATCTVKNGKAVCEC-QRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCD---RCGQ----CRFGSLCEVE--TGRCVCPSECVESA--QPVCGSDGHTYASECELHVHAC-THQISLYVASAGHCQTCGETVCTFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEAD 789 HSP 2 Score: 160.229 bits (404), Expect = 1.128e-38 Identity = 162/718 (22.56%), Postives = 293/718 (40.81%), Query Frame = 1
Query: 2650 GYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV--------KPNCPHID-----FKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCG-PLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G+S S + ++ E KV + K ++ + A + L + + ++G++ Y+ + R D+L + L + +V++R D GSG L S V + GR H + + R R L +DG G +P L+ +G + T+ V G GC+ L I ++ L + Q + +++SS V PN H + V + + P + K + CQ + PC C S C C G G CE V+ S + N F + L L +++ LE+ FL +GLL + D F+S+ L+ HLE G + S E +G W V + + + L + P+ + + +P LN+ +P++ GG F+ + ++ F GAIQ + + + Q + ++ + G PC + +PC G C+P C C L + + + + G++ +Y + +K+ N+ + +T + G+ +L + ++ +AI +G ++++ +LG + VLR T ++ N+W V+ R +R L + + A + ++ L TDG +WLGG +K PV + P Y T F GC+ ++ V +L+ DA
Sbjct: 1234 GFSCSCTAGRAGTVCE-KVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNA-RGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDIN---NQQLELSDWQRA-VVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEK--NPCQPN-PCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNL--------------KEPLYVGGAP--DFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLN----GGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETL-AFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGL-VLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRST--VKVNTNRWLRVRAHREHREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDA 1915 HSP 3 Score: 137.887 bits (346), Expect = 6.455e-32 Identity = 150/586 (25.60%), Postives = 217/586 (37.03%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGK---------STCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGI----------CNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCG--TDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC C FGA C G AVC C C G VCGSDG+ Y S C ++ C L + I+V+ C R + G C+ ++ C CP++ C S+ +CG+D TY + CE+ +C + G C C C F +C + C CP C PVCGSD VTY S C L+ +C++++ + G C+ + + C C + C CD+ C++ + PVCG+DG+ Y +C L K CE + E++V C P + C PY C+ + STC C N +Y C+ C + +P + + GI C+ +PR + DC + + C C + G+ CG DG+ H+ A T + C C F + C QC+CPT C VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 358 QTPSPCRGAQCAFGATCTVKNGK---AVCECQRV-CSGGYD--PVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFG------SLCEVET------------GRCVCPSE-CVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDF-SCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHC--------------NPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVR-DDC--------EQMTGLCSC----RPGVAGPKCGQCPDGQALG---HLGCEADPTTPVT-----------CVEMHCEFGASCVEEAGFA-QCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCR 865 HSP 4 Score: 85.1149 bits (209), Expect = 7.719e-16 Identity = 84/377 (22.28%), Postives = 152/377 (40.32%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-------HIWKFKDNSFVIF---PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVT 3666
G C L+ G CP+G S G ++ EN A FL D SY + + +++ + + G++ Y+ TD D++++ L NR++E+R DLG GA ++S+ + +G + +ER R L + G +P T L+ +G A P + L+ V GF G + + + + L+ Q+ ++ + +V P H +VD PCLNGG C E Y C C G+ G+ CE+ + F +++ + +ES + Q E++ A GL+ +I +R ++++ + HL+++
Sbjct: 1481 GSAPCHVLSRGGAKCACPLGRS----GSFCETVLEN---AGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGA--PDFSKLARGAAVASGFDGAIQLVSLRGHQ-----LLTQE--HVLRAVDVAPFAGH-PCTQAVD-------------------------NPCLNGGSC-IPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERA-DYMALAIVDGHLQLS 1813 HSP 5 Score: 73.559 bits (179), Expect = 2.274e-12 Identity = 88/432 (20.37%), Postives = 167/432 (38.66%), Query Frame = 1
Query: 3367 KPCLNGGLCQ-TSSEEMYSCQCLPGYQGITCEEV-VSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIR---FQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPK-------------HENLCIRNSMQH------------------------DNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PCL+GG CQ S + +SC C G G CE+V + + FK +SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L H++ T G L S+ G+W+ + + + + L+++ + + LN+++ ++ GG+ + + S+ G I+ + IN++ + +Q + + + + + PC P +PC + E +C C P N C ++ H +N + F + G S + + K + + F G+L+ + K F+ +A+ N +E +LG ++R + I+ W V + RN R L + D
Sbjct: 1218 QPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFP--TLRAYHTLRLALEFRALETEGLLLY--NGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTK--------------LYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSP--NPCHGGALCQAL---EAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIR--SKEPIALGTWVRVFLERNGRKGALQVGD 1624 HSP 6 Score: 59.3066 bits (142), Expect = 5.965e-8 Identity = 65/288 (22.57%), Postives = 110/288 (38.19%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYP-----YCKCPND-KCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIES--CKKKISLTVMYKGKCQTKIQSDI-CSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGV 1497
VCGSDG+ Y + C +K+ C ++ + V+ C R + ++ Q PY C N CP+ C+ S Y+ C+ C + RCD C+ F GI G T C C +P + + +T C + C + + C+ + + C + C +GA C++ + AQC C L C VCG+DG+ Y N+C L ++C +++++ + + C + GV
Sbjct: 612 VCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-RGPTLAPLLPMASPHCAQT----PYGCCQDNVTAAQGVGLAGCPSTCHCNPHGS---------YSGTCDPVTGQCSCRPGVGGL---RCDRCEPGFWNFRGIVTDGHSGC-TPCSC-DPRGAVRDDCE-------QMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAG-FAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGV 872
BLAST of Agrin, putative vs. Ensembl Mouse
Match: Agrn (agrin [Source:MGI Symbol;Acc:MGI:87961]) HSP 1 Score: 310.457 bits (794), Expect = 6.875e-85 Identity = 234/780 (30.00%), Postives = 350/780 (44.87%), Query Frame = 1
Query: 514 TSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKN----IQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGK-MCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-------PSVI---------------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFT------ENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
T+ D C + C FGA C+ +P A C C C A VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TCV + C CP + +CG+D + Y + C++ + +C I +++ G CD C+ + N IC + + +P+ L P+CG D VTY+++C + + + L + G+CQT+ D C C + + C+ CSCD + C +R PVC DG YDN C+ + C +Q + + C+ +PC G +C + NGK+ CEC + C PVCG+D VTY S+C L AC + + + G C+ RC + C +C+ + +C CP+ C +S + VCG+DG TY SEC + +HAC T+ I+L +A+ G C C C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED + +CR +G CVC C V K VCG+DG Y EC LK+ C + + VA P++ GC N A + +C CN GS + CDP T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+C+P G C CP G ++ + GC+ +
Sbjct: 57 TAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPA--MVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDP------------CANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNP-------RTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTR---DQCPET-CQFNSVCLSRRGR-PHCSCDRVTCDGAYR-PVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCA--FGATCTVKNGKAVCEC-QRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCD---RCGQ----CRFGSLCEVE--TGRCVCPSECVESA--QPVCGSDGHTYASECELHVHAC-THQISLYVASAGHCQTCGETVCTFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEAD 789 HSP 2 Score: 147.902 bits (372), Expect = 5.848e-35 Identity = 166/741 (22.40%), Postives = 296/741 (39.95%), Query Frame = 1
Query: 2650 GYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV--------KPNCPHID-----FKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCG-PLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYA-AVKQKR------------------DKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G+S S + ++ E KV + K ++ + A + L + + ++G++ Y+ + R D+L + L + +V++R D GSG L S V + GR H + + R R L +DG G +P L+ +G + T+ V G GC+ L I ++ L + Q + +++SS V PN H + V + + P + K + CQ + PC C S C C G G CE V+ S + N F + L L +++ LE+ FL +GLL + D F+S+ L+ HLE G + S E +G W V + + + L + P+ + K R +P LN+ +P++ GG F+ + ++ F GAIQ + + + Q + ++ + G PC + +PC G C+P C C L + + + + G++ +Y AV + +K+ N+ + +T + G+ +L + ++ +AI +G ++++ +LG + VLR T ++ N+W V+ R +R L + + A + ++ L TDG +WLGG +K PV + P Y T F GC+ ++ V +L+ DA
Sbjct: 1234 GFSCSCTAGRAGTVCE-KVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNA-RGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDIN---NQQLELSDWQRA-VVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEK--NPCQPN-PCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNL--------------KEPLYVGGAP--DFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLN----GGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETL-AFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGL-VLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRST--VKVNTNRWLRVRAHREHREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDA 1938 HSP 3 Score: 137.887 bits (346), Expect = 6.714e-32 Identity = 150/586 (25.60%), Postives = 217/586 (37.03%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGK---------STCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGI----------CNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCG--TDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC C FGA C G AVC C C G VCGSDG+ Y S C ++ C L + I+V+ C R + G C+ ++ C CP++ C S+ +CG+D TY + CE+ +C + G C C C F +C + C CP C PVCGSD VTY S C L+ +C++++ + G C+ + + C C + C CD+ C++ + PVCG+DG+ Y +C L K CE + E++V C P + C PY C+ + STC C N +Y C+ C + +P + + GI C+ +PR + DC + + C C + G+ CG DG+ H+ A T + C C F + C QC+CPT C VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 358 QTPSPCRGAQCAFGATCTVKNGK---AVCECQRV-CSGGYD--PVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFG------SLCEVET------------GRCVCPSE-CVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDF-SCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHC--------------NPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVR-DDC--------EQMTGLCSC----RPGVAGPKCGQCPDGQALG---HLGCEADPTTPVT-----------CVEMHCEFGASCVEEAGFA-QCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCR 865 HSP 4 Score: 75.8702 bits (185), Expect = 4.483e-13 Identity = 92/426 (21.60%), Postives = 163/426 (38.26%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFL----TNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-------HIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIG-KWNHVVILKAAKYIYLNLNHSPQPIKKR 3807
G C L+ G CP+G S G ++ EN A FL D SY + + +++ + + G++ Y+ TD D++++ L NR++E+R DLG GA ++S+ + +G + +ER R L + G +P T L+ +G A P + L+ V GF G + + + + L+ Q+ ++ + +V P H +VD PCLNGG C E Y C C G+ G+ CE+ + F +++ + L + E+T A L D F L NH E++ RT T+ + + +G + +++ + ++ L+ + QP+ R
Sbjct: 1481 GSAPCHVLSRGGAKCACPLGRS----GSFCETVLEN---AGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGA--PDFSKLARGAAVASGFDGAIQLVSLRGHQ-----LLTQE--HVLRAVDVAPFAGH-PCTQAVD-------------------------NPCLNGGSC-IPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEY------LNAVTESELTNEIPAPETL--------DSRALFSEKALQSNHFELSLRTE--ATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLR 1847 HSP 5 Score: 73.9442 bits (180), Expect = 2.222e-12 Identity = 88/432 (20.37%), Postives = 167/432 (38.66%), Query Frame = 1
Query: 3367 KPCLNGGLCQ-TSSEEMYSCQCLPGYQGITCEEV-VSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIR---FQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPK-------------HENLCIRNSMQH------------------------DNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PCL+GG CQ S + +SC C G G CE+V + + FK +SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L H++ T G L S+ G+W+ + + + + L+++ + + LN+++ ++ GG+ + + S+ G I+ + IN++ + +Q + + + + + PC P +PC + E +C C P N C ++ H +N + F + G S + + K + + F G+L+ + K F+ +A+ N +E +LG ++R + I+ W V + RN R L + D
Sbjct: 1218 QPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFP--TLRAYHTLRLALEFRALETEGLLLY--NGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTK--------------LYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSP--NPCHGGALCQAL---EAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIR--SKEPIALGTWVRVFLERNGRKGALQVGD 1624 HSP 6 Score: 58.9214 bits (141), Expect = 6.610e-8 Identity = 67/298 (22.48%), Postives = 109/298 (36.58%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTN-----PYPYCKCPND-KCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDEL------------CTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDI-CSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGV 1497
VCGSDG+ Y + C +K+ C ++ + V+ C R + ++ Q PY C N CP+ C+ S Y+ C+ C + RCD C+ F GI G T C C + C + P CG C +L + C+ + + C + C +GA C++ + AQC C L C VCG+DG+ Y N+C L ++C +++++ + + C + GV
Sbjct: 612 VCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-RGPTLAPLLPMASPHCAQT----PYGCCQDNVTAAQGVGLAGCPSTCHCNPHGS---------YSGTCDPVTGQCSCRPGVGGL---RCDRCEPGFWNFRGIVTDGHSGC-TPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQ---------------CPDGQALGHL---GCEADPTTPVTCVEMHCEFGASCVEEAG-FAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGV 872
BLAST of Agrin, putative vs. Ensembl Mouse
Match: Agrn (agrin [Source:MGI Symbol;Acc:MGI:87961]) HSP 1 Score: 298.901 bits (764), Expect = 8.274e-83 Identity = 248/851 (29.14%), Postives = 372/851 (43.71%), Query Frame = 1
Query: 337 ILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKN----IQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGK-MCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-------PSVI---------------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFT------ENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVEL 2721
+LVR ++I C I + L T F L + + G+ T+ D C + C FGA C+ +P A C C C A VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TCV + C CP + +CG+D + Y + C++ + +C I +++ G CD C+ + N IC + + +P+ L P+CG D VTY+++C + + + L + G+CQT+ D C C + + C+ CSCD + C +R PVC DG YDN C+ + C +Q + + C+ +PC G +C + NGK+ CEC + C PVCG+D VTY S+C L AC + + + G C+ RC + C +C+ + +C CP+ C +S + VCG+DG TY SEC + +HAC T+ I+L +A+ G C C C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED + +CR +G CVC C V K VCG+DG Y EC LK+ C + + VA P++ GC N A + +C CN GS + CDP T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+C+P G C CP G ++ + GC+ + V VE+
Sbjct: 18 VLVRY--FMIPCNI--------CLILLATSTLGFAVLLFLSNYKPGIH----------FTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPA--MVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDP------------CANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNP-------RTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTR---DQCPET-CQFNSVCLSRRGR-PHCSCDRVTCDGAYR-PVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCA--FGATCTVKNGKAVCEC-QRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCD---RCGQ----CRFGSLCEVET--GRCVCPSECVESA--QPVCGSDGHTYASECELHVHAC-THQISLYVASAGHCQTCGETVCTFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPT--TPVTCVEM 799 HSP 2 Score: 129.413 bits (324), Expect = 1.847e-29 Identity = 150/586 (25.60%), Postives = 217/586 (37.03%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGK---------STCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGI----------CNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCG--TDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC C FGA C G AVC C C G VCGSDG+ Y S C ++ C L + I+V+ C R + G C+ ++ C CP++ C S+ +CG+D TY + CE+ +C + G C C C F +C + C CP C PVCGSD VTY S C L+ +C++++ + G C+ + + C C + C CD+ C++ + PVCG+DG+ Y +C L K CE + E++V C P + C PY C+ + STC C N +Y C+ C + +P + + GI C+ +PR + DC + + C C + G+ CG DG+ H+ A T + C C F + C QC+CPT C VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 358 QTPSPCRGAQCAFGATCTVKNGK---AVCECQRV-CSGGYD--PVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFG------SLCEVET------------GRCVCPSE-CVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDF-SCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHC--------------NPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVR-DDC--------EQMTGLCSC----RPGVAGPKCGQCPDGQALG---HLGCEADPTTPVT-----------CVEMHCEFGASCVEEAGFA-QCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCR 865 HSP 3 Score: 55.4546 bits (132), Expect = 6.525e-7 Identity = 65/289 (22.49%), Postives = 110/289 (38.06%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYP-----YCKCPND-KCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIES--CKKKISLTVMYKGKCQTKIQSDI-CSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVK 1500
VCGSDG+ Y + C +K+ C ++ + V+ C R + ++ Q PY C N CP+ C+ S Y+ C+ C + RCD C+ F GI G T C C +P + + +T C + C + + C+ + + C + C +GA C++ + AQC C L C VCG+DG+ Y N+C L ++C +++++ + + C + GV
Sbjct: 612 VCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-RGPTLAPLLPMASPHCAQT----PYGCCQDNVTAAQGVGLAGCPSTCHCNPHGS---------YSGTCDPVTGQCSCRPGVGGL---RCDRCEPGFWNFRGIVTDGHSGC-TPCSC-DPRGAVRDDCE-------QMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAG-FAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVS 873
BLAST of Agrin, putative vs. Ensembl Mouse
Match: Egflam (EGF-like, fibronectin type III and laminin G domains [Source:MGI Symbol;Acc:MGI:2146149]) HSP 1 Score: 127.487 bits (319), Expect = 7.132e-29 Identity = 148/668 (22.16%), Postives = 271/668 (40.57%), Query Frame = 1
Query: 2764 NAENYLSINISLVPYSSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQT----HKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-HIWKFKDN----SFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENL-CIRNSMQH--DNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVD-EISYNLVLDA 4722
N+ + + + DG++ Y +++ D++++ L R + +R + G+G + S ++ +G H++ + R + L ++ G + + + +G A P L GF GC+ SL + + +++P P N DV E C + C++GG C + Y C C G++G CE+ + I +F+++ + +P E + EITF P + +G+L + D ++ F+S+ + H+E G L S +G+W+ + + + AK L ++ Q + + + GF + N IF GGV N + N L P F G+IQKI++ND I ++ N+ P G +PC+ G C P C C E L C + H + +++G+S Y + S +++N ++FKT + DG+L+ S + + +R+G + + NLG +GV I N + SD +WH V+ +R+ ++ + +DD R L +G +++GG K+ +Y GCIS + + +LV DA
Sbjct: 404 NSYQAFQVTLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA--PSAYWLVRATGTNRGFQGCVQSLSVNG-----------------KKIDMRP-WPLGKALNGADVGE------------------CSSGICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTGSKD--FLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQVDK--QKVVEGMAEGGFTQIKC------------NTDIFIGGVP-NYDDVKKNSGILHP-FSGSIQKIILNDRTIHVKHDFTSGVNVENAAHPCVG---APCA----HGGSCRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLG--SGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGK-SPGLMRQLNINGALYVGGMKEIALHTNRQYLRGLVGCISHFTLSTDYHISLVEDA 1005 HSP 2 Score: 82.4185 bits (202), Expect = 3.718e-15 Identity = 98/405 (24.20%), Postives = 162/405 (40.00%), Query Frame = 1
Query: 2605 CKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLK-----DSFAKSYWSGNAENYLSIN---ISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCE-EVVSH----------IWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILK 3750
C + Y +CP+G+ C+ DA L + S+A + W ++YLS I+ P S DG++ Y DT S D+L+I + +VE+R D GSG L+S LT+G+ H + V R ++ L +D GM T + IG P + + + F G + + ++N +T + DF + V+V EN + PC +GG C+ +E Y C C G++G+ C+ E +H I +F S++ + + ++ R R + F A +GLL + D R N FIS+ L L + GV ++ S G+W+ V ++
Sbjct: 580 CAAIKADSYICLCPLGFR--GRHCE---------DAFALTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTG-SKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGV--PNYDDVKKNSGILHPFSGSIQKI-----------ILNDRTIHVKH-----------DFTSGVNV------------ENAA---HPCVGAPCAHGGSCR-PRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSGVASIMVNGSFSDGRWHRVKAVR 932
BLAST of Agrin, putative vs. UniProt/SwissProt
Match: sp|P25304|AGRIN_RAT (Agrin OS=Rattus norvegicus OX=10116 GN=Agrn PE=1 SV=2) HSP 1 Score: 317.775 bits (813), Expect = 2.364e-86 Identity = 232/779 (29.78%), Postives = 357/779 (45.83%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKN----IQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK------LQFIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-------PSVI---------------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFT------ENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSI 2691
D C + C FGA C+ +P A C C C A VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TCV + C CP + +CG+D + Y + C++ +C + I +++ G CD C+ + N IC + + +P+ L P+CG D VTY+++C + + + L + G+CQT+ D C C + + C+ CSCD + C ++R PVC DG Y+N C+ + C +Q + + C+ +PC GV+C + NGK+ CEC + C I PVCG+D VTY S+C L AC + + + G C+ +C+ G C ++ +C CP+ C +S + VCG+DG TY SEC + +HAC T+ I+L +A+ G C C K C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ +++ C ++ +CR +G CVC C V + VCG+DG Y EC+LK+ C + + VA P++ GC N+ A + +C CN GS + CDP+T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+C+P G C CP G + + GC+ + +
Sbjct: 65 DVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPA--TVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDP------------CANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNP-------RTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTR---DQCPET-CQFNSVCLSRRGR-PHCSCDRVTCDGSYR-PVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCA--FGAVCTVKNGKAECEC-QRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRF-GSLC--------EVETGRCVCPSECVESA--QPVCGSDGHTYASECELHVHAC-THQISLYVASAGHCQTCGEKVCTFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQE-----LCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPM 791 HSP 2 Score: 141.354 bits (355), Expect = 4.651e-32 Identity = 161/742 (21.70%), Postives = 294/742 (39.62%), Query Frame = 1
Query: 2650 GYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSV------DVIE--------DPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCG-PLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKY--AAVKQK-----------------RDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G++ S + S+ E KV + K ++ + A + L + + ++G++ Y+ + R D+L + L + V++R D GSG L S V + GR H + + R R L +DG G +P L+ +G + + V G GC+ L I ++ L + Q + ++SS V H N +E P + + + K CQ + PC C+ S C+C G G C+ V+ S + N F + L L +++ LE+ FL +GLL + D F+S+ L+ HLE G + S E +G W V + + + L + P+ + K R +P LN+ +P++ GG F+ + ++ F G IQ + + + Q + +++ + PC L +PC G CVP C C L + + + + G++ +Y A ++ + +K+ N+ + +T + G+ +L + + ++ +AI +G ++++ +LG + VLR T ++ N+W ++ R +R L + + A + ++ L TDG +WLGG +K PV + P Y T F GC+ ++ V +L+ DA
Sbjct: 1243 GFTCSCTAGRGGSVCE-KVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNA-RGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDIN---NQQLELSDWQRA-AVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADE---KSPCQPN-PCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNL--------------KEPLYIGGAP--DFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLN----GGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETL-AFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGL-VLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRST--VKVNTNRWLRIRAHREHREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDA 1947 HSP 3 Score: 134.035 bits (336), Expect = 6.289e-30 Identity = 155/589 (26.32%), Postives = 221/589 (37.52%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVS---PNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN---NPCSGVKCPD----PYQYCRIDNNGK---------STCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGI----------CNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCG--TDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC + C FGA C G A C C C VCGSDG+ Y S C ++ C L + IQV+ P CG+ C+ + + C CP++ C S+ +CG+D TY + CE+ +C + G C C C F +C + C CP C PVCGSD VTY S C L+ +C++++ + + G C+ + D C C + C CD+ C++ R PVCG+DG+ Y +C L K CE Q E++V C P V P PY C+ + STC C N +Y C+ C + +P + + GI C+ +PR + DC + + C C + G+ CG DG+ H+ CE + + T C C F + C + + QCICPT C VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 358 QTPSPCHGVQCAFGAVCTVKNGK---AECECQRV-CSGIYD--PVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQ---------------CR------FGSLCEVETGRCVCPSE-CVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDF-SCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHC--------------NPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVR-DDC--------EQMTGLCSC----RPGVAGPKCGQCPDGQVLG---HL---GCEADPM--------TPVTCVEIHCEFGASC-VEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQ 865 HSP 4 Score: 68.9366 bits (167), Expect = 4.156e-10 Identity = 88/425 (20.71%), Postives = 158/425 (37.18%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFL----TNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-------HIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKR 3807
G C+ L+ G CP+G S G ++ E A FL D SY + + +++ + + G++ Y+ TD D++++ L NR++E+ DLG GA ++S+ + +G + +ER R L + G +P T L+ IG A P + L+ V+ GF G + + + + L+ Q+ ++ + +V P H + PCLNGG C E Y C C G+ G+ CE+ + F +++ + L ++ E+T A L D F L NH E++ RT L+ + +++ + ++ L+ + QP+ R
Sbjct: 1490 GAAPCRVLSSGGAKCECPLGRS----GTFCQTVLET---AGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGA--PDFSKLARGAAVSSGFSGVIQLVSLRGHQ-----LLTQE--HVLRAVDVSPFADHPCTQ--------------------------ALGNPCLNGGSC-VPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEY------LNAVIESELTNEIPAPETL--------DSRALFSEKALQSNHFELSLRTE-ATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLR 1856 HSP 5 Score: 65.0846 bits (157), Expect = 5.950e-9 Identity = 86/432 (19.91%), Postives = 162/432 (37.50%), Query Frame = 1
Query: 3367 KPCLNGGLCQ-TSSEEMYSCQCLPGYQGITCEEV-VSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIR---FQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSP--------KHENLCIRNSMQHDNP-----------------------------ANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PCL+GG CQ S + ++C C G G CE+V + FK +SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L ++ T G L S+ G+W+ + + + + L+++ + + LN+++ ++ GG+ + S+ G I+ + IN++ + +Q + + + + + PC L +PC + E +C C P ++ C N P + F + G S + + K + + F G+L+ + K F+ +A+ N +E +LG V+R + I+ W V + RN R L + D
Sbjct: 1227 QPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFP--TLRAYHTLRLALEFRALETEGLLLY--NGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTN--------------LYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPC--LPNPCHGGALCQAL---EAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIR--SKEPIALGTWVRVFLERNGRKGALQVGD 1633 HSP 6 Score: 59.6918 bits (143), Expect = 2.936e-7 Identity = 63/285 (22.11%), Postives = 110/285 (38.60%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHIC-GRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEI-------TYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIES--CKKKISLTVMYKGKCQTKIQSDI-CSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN 1482
VCGSDG+ Y + C +K+ C Q+ + V+ C G A ++ + C PY C+ ++ + + G +Y+ C+ C + RCD C+ F GI G T C C +P + + +T C + C + V+ C+ + + C I C +GA C++ + AQC C L C VCG+DG+ Y N+C L ++C +++++ + C+ +
Sbjct: 612 VCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVA--------------FPHCAQTPYGCCQ--DNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGL---RCDRCEPGFWNFRGIVTDGHSGC-TPCSC-DPRGAVRDDCE-------QMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAG-FAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQES 867
BLAST of Agrin, putative vs. UniProt/SwissProt
Match: sp|O00468|AGRIN_HUMAN (Agrin OS=Homo sapiens OX=9606 GN=AGRN PE=1 SV=6) HSP 1 Score: 315.079 bits (806), Expect = 1.673e-85 Identity = 256/907 (28.22%), Postives = 391/907 (43.11%), Query Frame = 1
Query: 205 DLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIM-------YLKKTEQNNFF--------ELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNI----QVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPED--CPLGKMCR-----------NGQCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIRVA-------------PSVI---------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
++++ G V + N + + V V R LKG DL+ + N +++ + C + +G+ I YL +N L +E + +K + F T D C + C FGA C+ P A C C + C + VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TC + C CP + +CG+D Y C++ + +C + +++ G CD C+ + + C + + P+ L Q PVCG D VTY+++C + + + L + G+CQ + D C C + A C+ +CSCD + C +R PVC DG YD+ C+ + C +Q + + C+ +PC GV+C + NG++ CEC + C + PVCG+D VTY S C L TAC + + + G C+ RC + C +C+++ +C CP+ C L + VCG+DG TY SEC + +HAC T+ I+L +A+ G C+ C + C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED C ++CR +G CVC C V VCG+DG Y ECELK+ C + + VA P V GC N A +C CN GS CDP+T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+CKP G C CP G ++ GC+ ++
Sbjct: 44 NVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNK---VVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHF----TPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPS--VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDP------------CSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNP-------RTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGR---DQCPE-PCRFNAVCLSRRGR-PRCSCDRVTCDGAYR-PVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCA--FGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCD---RCGQ----CRFGALCEAE--TGRCVCPSEC--VALAQPVCGSDGHTYPSECMLHVHAC-THQISLHVASAGPCETCGDAVCAFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQ-ELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADA 895 HSP 2 Score: 160.229 bits (404), Expect = 7.940e-38 Identity = 179/652 (27.45%), Postives = 255/652 (39.11%), Query Frame = 1
Query: 493 SVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATC-VTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP------YQYCRI--------DNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEE------PRCKEQGKDCPSYQICKSDLVQAK----CECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRF------LEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE-DCPLGKMC----RNGQCVCTD-KCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
+ L S Q Q D C + C+F A C G P C C C + VC DG YDS C ++ EC Q++I ++ G C C ATC V N C+C C S +CG+D +TY + CE++ +C R I+ G CD C +C F +CE E G C CP+ C Q PVCGSD TY SEC L + +C +ISL V G C+T C + C +GA C + QC C C++ PVCG+DG+ Y + C L + +C +Q ++ + CE C + CR +G C+ + P PVCG+D VTY + C L + C +++ L++ G C P P Y C+ ++ A+ CP+ Q C G +Y C C S + + CD+C+ +F I R +PQ DC + +C +C C + R + GP + C E C G C + CVC C + +VCG+DG Y NEC+LK AC I +
Sbjct: 378 GLLLQKVRSGQCQGRDQCPE-PCRFNAVCLSRRGRP---RCSCDRVTCDGAYR--PVCAQDGRTYDSDCWRQQAECRQQRAI-----------PSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACEC-LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCG--QCRFGALCEA-ETGR---CVCPS---ECVALAQ-PVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCET------CGDAVCAFGAVC-----SAGQCVCPR--CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPG--SPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTP-YGCCQDNITAARGVGLAGCPSACQ-------CNPHG-SYGGTCDPATGQC---SCRPGVGGL-RCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTG-LCSCKPGVAGPKC----GQCPDGRAL-----GPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISI 963 HSP 3 Score: 127.102 bits (318), Expect = 1.027e-27 Identity = 168/755 (22.25%), Postives = 282/755 (37.35%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLS---------SLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQS-PCSKTDPEKGFCVPEMNETICTC----SPKH--ENLCIRNSMQHDNPA-NTNFFRKYLGKSASKYAA----VKQKRD-------KSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G C L G CS CP G + ++ E + A F SF ++ + A + L + + G++ Y+ + R D+L + L + V+ R D GSG L S V + G+ H + + R R L +DG G +P L+ +G V G GC+ L I G T+ + + + P + + + P + K CQ + PC C+ E C+C G +G C+ S + N F L L +++ LE+ FL +GLL + D F+S+ L LE G + S E +G W V + + + L + P+ + K R +P LN+ +P++ GG F+ + ++ F GAIQ + + + + ++T + G PC PC CVP +C C S H + L +++ D A + F +YL A V + D K+ N+ + +T + G+ +L + + ++ +AI +G ++++ NLG + VLR T ++ N+W V R R L + + A + ++ L TDG +WLGG + PV P Y T F GC+ ++ V +L+ DA
Sbjct: 1342 GTCQDWALGGGFTCS-CPAG--------RGGAVCEKVLGAPVPAFEGRSFL-AFPTLRAYHTLRLALEFRALEPQGLLLYNGNA-RGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEK--SPCQPN-PCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNL--------------KEPLYVGGAP--DFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLN----GASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHSEKALQSNHFELSLRTEATQGL-VLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRST--VPVNTNRWLRVVAHREQREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDA 2056 HSP 4 Score: 125.946 bits (315), Expect = 1.885e-27 Identity = 147/576 (25.52%), Postives = 211/576 (36.63%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQ---ICKSDLVQAKC-ECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC + C FGA C G A C C VCGSDG+ Y S C ++ C L + IQV+ C R + G C+ ++ C CP++ C + + +CG+D TY + C + +C + G C+ C C F +C + C CP C PVCGSD VTY S C L+ +C ++ + G C+ + C C + C CD+ C++ PVCG+DG+ Y +C L K CE Q ++V C P + + C PY C+ +N + CP Q C N +Y C+ C + +P + + GI + C QG DC CK + KC +CP LG + C D + MR CE + +C + S + +C P CP VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 463 QAPSPCLGVQCAFGATCAVKNGQ---AACECLQA---CSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGA------LCEAETG------------RCVCPSE-CVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDF-SCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQ--DNITAARGVGLAGCPSACQ--C-NPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPD--GRALGPAGCEADASAPATCAEMR---CEFGA------------RCVEESGSAHCVC-------PMLTCPEANATK---VCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQ 970
BLAST of Agrin, putative vs. UniProt/SwissProt
Match: sp|A2ASQ1|AGRIN_MOUSE (Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1) HSP 1 Score: 311.227 bits (796), Expect = 2.263e-84 Identity = 234/780 (30.00%), Postives = 350/780 (44.87%), Query Frame = 1
Query: 514 TSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKN----IQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN--NPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGK-MCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVA-------PSVI---------------GCISHNYIADEKN-----KRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFT------ENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
T+ D C + C FGA C+ +P A C C C A VCGSD Y + C ++R +C Q+ I++ CG ++ C + +C +TCV + C CP + +CG+D + Y + C++ + +C I +++ G CD C+ + N IC + + +P+ L P+CG D VTY+++C + + + L + G+CQT+ D C C + + C+ CSCD + C +R PVC DG YDN C+ + C +Q + + C+ +PC G +C + NGK+ CEC + C PVCG+D VTY S+C L AC + + + G C+ RC + C +C+ + +C CP+ C +S + VCG+DG TY SEC + +HAC T+ I+L +A+ G C C C+F ++C QC+CP PVCGSD TY + C L+ +C + I GPC+ ED + +CR +G CVC C V K VCG+DG Y EC LK+ C + + VA P++ GC N A + +C CN GS + CDP T QC C+ G+ G CDRC G+WNF +GCT CSC+ G+ C TG C+C+P G C CP G ++ + GC+ +
Sbjct: 57 TAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPA--MVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDP------------CANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNP-------RTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTR---DQCPET-CQFNSVCLSRRGR-PHCSCDRVTCDGAYR-PVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCA--FGATCTVKNGKAVCEC-QRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCD---RCGQ----CRFGSLCEVE--TGRCVCPSECVESA--QPVCGSDGHTYASECELHVHAC-THQISLYVASAGHCQTCGETVCTFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEAD 789 HSP 2 Score: 147.902 bits (372), Expect = 4.022e-34 Identity = 166/741 (22.40%), Postives = 296/741 (39.95%), Query Frame = 1
Query: 2650 GYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV--------KPNCPHID-----FKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESLQ-----LRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCG-PLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYA-AVKQKR------------------DKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK-PV-EGFPWEYNTKFKGCISELYVDEISYNLVLDA 4722
G+S S + ++ E KV + K ++ + A + L + + ++G++ Y+ + R D+L + L + +V++R D GSG L S V + GR H + + R R L +DG G +P L+ +G + T+ V G GC+ L I ++ L + Q + +++SS V PN H + V + + P + K + CQ + PC C S C C G G CE V+ S + N F + L L +++ LE+ FL +GLL + D F+S+ L+ HLE G + S E +G W V + + + L + P+ + K R +P LN+ +P++ GG F+ + ++ F GAIQ + + + Q + ++ + G PC + +PC G C+P C C L + + + + G++ +Y AV + +K+ N+ + +T + G+ +L + ++ +AI +G ++++ +LG + VLR T ++ N+W V+ R +R L + + A + ++ L TDG +WLGG +K PV + P Y T F GC+ ++ V +L+ DA
Sbjct: 1234 GFSCSCTAGRAGTVCE-KVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNA-RGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDIN---NQQLELSDWQRA-VVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEK--NPCQPN-PCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNL--------------KEPLYVGGAP--DFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLN----GGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETL-AFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGL-VLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRST--VKVNTNRWLRVRAHREHREGSLQVGN---EAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDA 1938 HSP 3 Score: 138.658 bits (348), Expect = 3.027e-31 Identity = 150/586 (25.60%), Postives = 217/586 (37.03%), Query Frame = 1
Query: 529 QNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK---------IQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN------PCSGVKCPD-PYQYCRIDNNGK---------STCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGI----------CNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCG--TDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK 2136
Q PC C FGA C G AVC C C G VCGSDG+ Y S C ++ C L + I+V+ C R + G C+ ++ C CP++ C S+ +CG+D TY + CE+ +C + G C C C F +C + C CP C PVCGSD VTY S C L+ +C++++ + G C+ + + C C + C CD+ C++ + PVCG+DG+ Y +C L K CE + E++V C P + C PY C+ + STC C N +Y C+ C + +P + + GI C+ +PR + DC + + C C + G+ CG DG+ H+ A T + C C F + C QC+CPT C VCGSD TY NEC LK +C + I+ S GPC+
Sbjct: 358 QTPSPCRGAQCAFGATCTVKNGK---AVCECQRV-CSGGYD--PVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFG------SLCEVET------------GRCVCPSE-CVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQ------CVCPR----CEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDF-SCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHC--------------NPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVR-DDC--------EQMTGLCSC----RPGVAGPKCGQCPDGQALG---HLGCEADPTTPVT-----------CVEMHCEFGASCVEEAGFA-QCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCR 865 HSP 4 Score: 75.8702 bits (185), Expect = 3.136e-12 Identity = 92/426 (21.60%), Postives = 163/426 (38.26%), Query Frame = 1
Query: 2593 GKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSY--------WSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFL----TNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-------HIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIG-KWNHVVILKAAKYIYLNLNHSPQPIKKR 3807
G C L+ G CP+G S G ++ EN A FL D SY + + +++ + + G++ Y+ TD D++++ L NR++E+R DLG GA ++S+ + +G + +ER R L + G +P T L+ +G A P + L+ V GF G + + + + L+ Q+ ++ + +V P H +VD PCLNGG C E Y C C G+ G+ CE+ + F +++ + L + E+T A L D F L NH E++ RT T+ + + +G + +++ + ++ L+ + QP+ R
Sbjct: 1481 GSAPCHVLSRGGAKCACPLGRS----GSFCETVLEN---AGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGA--PDFSKLARGAAVASGFDGAIQLVSLRGHQ-----LLTQE--HVLRAVDVAPFAGH-PCTQAVD-------------------------NPCLNGGSC-IPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEY------LNAVTESELTNEIPAPETL--------DSRALFSEKALQSNHFELSLRTE--ATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLR 1847 HSP 5 Score: 73.559 bits (179), Expect = 1.649e-11 Identity = 88/432 (20.37%), Postives = 167/432 (38.66%), Query Frame = 1
Query: 3367 KPCLNGGLCQ-TSSEEMYSCQCLPGYQGITCEEV-VSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIR---FQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPK-------------HENLCIRNSMQH------------------------DNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
+PCL+GG CQ S + +SC C G G CE+V + + FK +SF+ FP +L+ L+L + F L GLL + +G+ R F+++ L H++ T G L S+ G+W+ + + + + L+++ + + LN+++ ++ GG+ + + S+ G I+ + IN++ + +Q + + + + + PC P +PC + E +C C P N C ++ H +N + F + G S + + K + + F G+L+ + K F+ +A+ N +E +LG ++R + I+ W V + RN R L + D
Sbjct: 1218 QPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFP--TLRAYHTLRLALEFRALETEGLLLY--NGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTK--------------LYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSP--NPCHGGALCQAL---EAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIR--SKEPIALGTWVRVFLERNGRKGALQVGD 1624 HSP 6 Score: 59.3066 bits (142), Expect = 3.746e-7 Identity = 65/288 (22.57%), Postives = 110/288 (38.19%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTN-----PYPYCKCPND-KCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIES--CKKKISLTVMYKGKCQTKIQSDI-CSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGV 1497
VCGSDG+ Y + C +K+ C ++ + V+ C R + ++ Q PY C N CP+ C+ S Y+ C+ C + RCD C+ F GI G T C C +P + + +T C + C + + C+ + + C + C +GA C++ + AQC C L C VCG+DG+ Y N+C L ++C +++++ + + C + GV
Sbjct: 612 VCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-RGPTLAPLLPMASPHCAQT----PYGCCQDNVTAAQGVGLAGCPSTCHCNPHGS---------YSGTCDPVTGQCSCRPGVGGL---RCDRCEPGFWNFRGIVTDGHSGC-TPCSC-DPRGAVRDDCE-------QMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAG-FAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGV 872
BLAST of Agrin, putative vs. UniProt/SwissProt
Match: sp|P31696|AGRIN_CHICK (Agrin OS=Gallus gallus OX=9031 GN=AGRN PE=1 SV=3) HSP 1 Score: 299.671 bits (766), Expect = 1.222e-80 Identity = 260/914 (28.45%), Postives = 396/914 (43.33%), Query Frame = 1
Query: 202 SDLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQ------NNFFELNYA----------------KEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEM-----MEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE---NNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT--CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRV---------------------APSVIGCISHNY-----IADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF------TENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKEN 2685
+++++ G V + N + + V V R LKG D++ + N +++ + C + +G+ I ++ Q N LN + +E L +K S F + + D C + C FGA C++ P +P A C C T C VCGSD Y + C +++ +C Q+ I+V CG K+ C +C +TCV + C CP + S +CG+D Y + C++ K +C + +++ G CD CK + + N +C + + L CP+ E PVCG D VTY SEC + + + + + G+CQ D C + +C + A C+K + A+CSCD + C T+R PVC D Y N C K C ++ + V +S C+ +PC V+C + + N + CEC ++ C PVCG+D TY + C L AC+ K+ + + + G C+ RC + C IC+++ +C CPT P S + VCGTDG TY SEC + + AC T + +A G C C CSF S C + QC+CP VCG+D TY+N C L+ SC + + I GPC+ + CR + +CVC C V + VCG+D Y NECELK+ C + + V + ++ GC N + +C CN GS CDP+T QC CK G+ G CDRC G+WNF +++GCT C+C+ GS C TG C+CK G C+ CP G + GC+++
Sbjct: 38 ANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGKDIVTHEILLDGGNK---VVIGGFGDPLICDNQVSTGDTRIFFVNPAPQYMWPAHRNELMLNSSLMRITLRNLEEVEHCVEEHRKLLADKPNSYFTQTPPTPR----DACRGMLCGFGAVCERSPTDPSQASCVCKKTACPV--VVAPVCGSDYSTYSNECELEKAQCNQQRRIKVISKGPCGSKDP------------CAEVTCSFGSTCVRSADGQTAGCVCPASCSGVAESIVCGSDGKDYRSECDLNKHACDKQENVFKKFDGACDPCKGILNDMNRVCRVNPRTRRVELLSRPENCPSKRE--------PVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQ---HQDKCKD-ECKFNAVCLKRWH--ARCSCDRITCDGTYR-PVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVEC--TFGATCVVKNREPVCEC-QQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCD---RCGK----CQFGAICEAET--GRCVCPTECVPSS---QPVCGTDGNTYGSECELHVRAC-TQQKNILVAAQGDCKSCGTTVCSFGSTC-----VGGQCVCPRCEQQPLAQVCGTDGLTYDNRCELRAASCQQQKSIEVAKMGPCEDECGSGGSGSGDGSECEQDRCRHYGGWWDEDAEDDRCVCDFTCLAVPRSPVCGSDDVTYANECELKKTRCEKRQNLYVTSQGACRALTTTPPPLPVVHCSQTIYGCCPDNMTLALGVGAAGCPSTCQCNPYGSYGGTCDPATGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDSKSGCTPCNCDPVGSVRDDCEQMTGLCSCKTGITGMKCNQCPNGSKMGMAGCEKD 894 HSP 2 Score: 145.976 bits (367), Expect = 1.932e-33 Identity = 167/726 (23.00%), Postives = 295/726 (40.63%), Query Frame = 1
Query: 2725 FLKDSFAKSYWSGNA-ENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNV--------KPNCPHIDFKNSVDVIEDPDKE---EQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS--HIWKF--KDNSFVIFPSESLQLR----QRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPI----KKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTC----SPKH-ENLCI--------------RNSMQHDNPANTNFFRKYL-GKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ KSY + + Y ++ I++ +++ G++ Y+ +R D++++ L +VE R + GSG + S+V++ G+ H ++V R R L +DG + G +P L+ +G A + ++ T G G + L + ++Y + ++ S ++ S V PN H V E E + ++ C PC C E C C G +G CE V H F + N F LQ +++ +E+ FL + +G++ + D F+S+ L+ +LE G L S E + W V++ ++ + + +N+ + + K R +P FLN+ +P + GG F+ + ++ +FYGA+Q+I I + + I+ ++ PC +PC G C P + C C S H E + I R M++ N + + A Y A + +K+ N+ + KT + G+ IL + ++ +AI +G +++ +LG K VLR T I+ N W ++ R R L + + A + ++ L TDG +WLGG ++ P Y+T F GCI ++ VD +LV DA N IL C
Sbjct: 1389 FIPSFGGKSYLAFKMMKAYHTVRIAMEFRATELSGLLLYNGQ-NRGKDFISLALVGGFVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVDG-EHVSGESPTGTDGLNLDTDLFVGGAPEDQMAVVAERTAATVGLKGSIRLLDVN----NQMYDLREKGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEMFHCECLHSYTGPTCADERNPCDP-TPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTLFLTCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNL--------------KEPFYVGGAP--DFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFRAHPCTQKPNPCQN----GGTCSPRLESYECACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSHLSNEIPAPDALDYPA--EPSEKALQSNHFELSIKTEATQGL-ILWSGKGLERSDYIALAIVDGFVQMMYDLGSKPVVLRST--VPINTNHWTHIKAYRVQREGSLQVGN---EAPITGSSPLGATQLDTDGALWLGGMERLSVAHKLPKAYSTGFIGCIRDVIVDRQELHLVEDALNNP-TILHC 2078 HSP 3 Score: 124.02 bits (310), Expect = 7.537e-27 Identity = 154/587 (26.24%), Postives = 216/587 (36.80%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKI-------------QSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN-----NPCSGVKCPDPYQYCRIDNNG----------KSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGI----------CNEEP------RCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSIC-QMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPC 2133
PC + C FGA C P VC C CQ + VCGSD Y + C + C L++ I+V C R Q G C+ ++ C CP + C SS +CGTD TY + CE+ +C + I G C C C F C +G C CP C + VCG+D +TY + C L+ SC+++ S+ V G C+ + + D C HYG + + +C CD+ C R PVCG+D + Y N+C L K CEK+ ++V + C P V C C DN STC+C N +Y C+ C K +P + + GI CN +P C++ C CK+ + KC CP M M CE + A K C+ C F + C ++N F +C P VCGSD TY ++C LK +C + +LIT G C
Sbjct: 469 PCLSVECTFGATCVVKNREP---VCECQQV-CQG--RYDPVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDRCGKCQFGA------ICEAET------------GRCVCPTE-CVPSSQPVCGTDGNTYGSECELHVRACTQQKNILVAAQGDCKSCGTTVCSFGSTC------VGGQCVCPR----CEQQPLAQVCGTDGLTYDNRCELRAASCQQQKSIEVAKMGPCEDECGSGGSGSGDGSECEQDRCR----HYGGWWDEDAED-DRCVCDFT-CLAVPRSPVCGSDDVTYANECELKKTRCEKRQNLYVTSQGACRALTTTPPPLPVVHCSQTIYGCCPDNMTLALGVGAAGCPSTCQC--------------NPYGSYGGTCDPATGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDSKSGCTPCNCDPVGSVRDDCEQMTGLCS----CKTGITGMKCN--QCPNGS-----------------KMGMAGCEKDPSAPK--------SCEEMSCEFGATCVEVNGFAHCECPSPLCSEANMTKVCGSDGVTYGDQCQLKTIACRQGQLITVKHVGQC 969 HSP 4 Score: 105.145 bits (261), Expect = 4.314e-21 Identity = 107/391 (27.37%), Postives = 160/391 (40.92%), Query Frame = 1
Query: 1543 KSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECP-TCPQSGLGES-VCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMN-RFLEPQCIC-PTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCR---NGQCVCTD-KCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSI 2691
+++C C K CP +V PVCG+D TY + C L + C ++ + +I G C + C E S + +D A C CP +C SG+ ES VCG+DGK Y+SEC + HAC+ K + G CD CK +C++N R + + P +C R PVCG D TY +EC + I V G C+ Q ++C ++C +C + +C C C VC D Y N+C E+ K C H+ K I ++ C G T + C + C FG+ +V N E C C+ + G+Y VC + C+ N++
Sbjct: 194 QASCVCKKTACPVVVAPVCGSDYSTYSNECELEKAQCNQQRRIKVISKGPCGSKDPCAEVTCSFGSTCVRSADGQTAGCVCPASC--SGVAESIVCGSDGKDYRSECDLNKHACDKQENVFKKFD-GACDPCKGILNDMNRVCRVNPRTRRVELLSRPENCPSKREPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQ------DKCKDECKFNAVCLKRWHARCSCDRITCDGTYRPVCARDSRTYSNDC------------------------------ERQKAEC---------HQ----------KAAIPVKHSGPCDLG----TPSPCLSVECT-FGATCVVKNREP-VCECQQVCQGRYDPVCGSDNRTYGNPCELNAM 520 HSP 5 Score: 78.5666 bits (192), Expect = 6.093e-13 Identity = 93/432 (21.53%), Postives = 167/432 (38.66%), Query Frame = 1
Query: 2575 VCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVE--LGFLKDSFAKSYWSGNAENYL-------SINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFI-----FTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFIS------DGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKR 3807
C E C+ P + C V P G ++ + +V + + FL + SY N L S+ + + S G+IFY+ TD D++++ L + Y+EYR DLG GA L+S+ + + S+L+ER R + I+ + G +P + F+ F +G A P + L+ ++ F G + + I + L+ +Q HI +++V++ + Q PC NGG C + E Y C C G+ G CE+V+ I K + +E++ R T++ N S +S D D L NH E++ +T LI + + +++ + ++ + + +P+ R
Sbjct: 1599 TCADERNPCDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTLFLTCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKS-RKVPHAFLNLKEPFYVGGA--PDFSKLARAAAISTSFYGAVQRISIKG-----VPLLKEQ---------------HI--RSAVEI------------STFRAHPCTQKPNPCQNGGTC-SPRLESYECACQRGFSGAHCEKVI--IEKAAGD------AEAIAFDGR-----TYMEYHNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTE-ATQGLILWSGKGLERSDYIALAIVDGFVQMMYDLGSKPVVLR 1978 HSP 6 Score: 70.4774 bits (171), Expect = 1.607e-10 Identity = 91/435 (20.92%), Postives = 175/435 (40.23%), Query Frame = 1
Query: 3355 CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDE--NIRFQNSDIILNN---------------------------------ITQWQGPPCGPLQSPCSKTD---PEKGFCVPEMNETICTCSPKHEN-LCIRNSMQ-HDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDD 4536
C +H PCL+GG C+ E ++C+C G G CE+ + + I F S++ F + ++ +++ + F +GLL + +G +R FIS+ L +E+ T G + S GKW+ +V+ + + L ++ + P G +N + +F GG + + + G+I+ + +N++ ++R + SD++ + + + GP C ++PC T +PE +C C E C R + Q H P F ++ G S + + Q + + ++ + F S GM+ + K F+ +A+ +G +E +LG VLR + + N W +V + R+ R V+ I++
Sbjct: 1351 CDSH-PCLHGGTCEDDGRE-FTCRCPAGKGGAVCEKPIRYFIPSFGGKSYLAF--KMMKAYHTVRIAMEFRATELSGLLLY--NGQNRGKDFISLALVGGFVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVDGEHVSGES---PTGTDGLN------------LDTDLFVGGAPEDQMAVVAERTAATVGLKGSIRLLDVNNQMYDLREKGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEMFHCECLHSYTGPTCADERNPCDPTPCHISATCLVLPE-GGAMCACPMGREGEFCERVTEQDHTMP----FLPEFNGFSYLELNGL-QTLFLTCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLR--SKEPVPLNTWISVLLERSGRKGVMRINN 1756
BLAST of Agrin, putative vs. UniProt/SwissProt
Match: sp|A3KN33|EGFLA_BOVIN (Pikachurin OS=Bos taurus OX=9913 GN=EGFLAM PE=2 SV=1) HSP 1 Score: 134.035 bits (336), Expect = 4.615e-30 Identity = 146/661 (22.09%), Postives = 270/661 (40.85%), Query Frame = 1
Query: 2764 NAENYLSINISLVPYSSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS-HIWKFKDN----SFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVD-EISYNLVLDA 4722
N+ I + + DG++ Y + + D++++ + R +++R + G+G + S ++ +G H++ + R + L ++ G + + + +G A P L GF GC+ +L + +D+ P + S + + C+NGG C S + Y C C G++G CE+ + I +FK++ + +P E ++ E+TF P + +G+L + D ++ F+S+ + H+E G L S E +G W+ + + + AK L ++ K++ V + TQ + N IF GGV N + N LKP F G+IQKI++ND I ++ N+ P G SPC+ G C P C C E L + ++ +++G+S Y + S +++N ++FKT + DG+L+ S + + +R+G + + NLG +GV I N + +D +WH V+ +R+ ++ + +DD R L +G +++GG K+ +Y GCIS + + +LV DA
Sbjct: 413 NSYQAFQITLEFRAEAEDGLLLYCGENEHGRGDFMSLAVIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA--PSAYWLVRATGTNRGFQGCVQALTVNG--------------------------------KRLDLRPWPLGKALSGADVGECSSGICDEASCINGGTCMASKADSYICLCPLGFRGRHCEDAFTLTIPQFKESLRSYAATPWPLEPRHYLSFMEFEVTFRPDSEDGVLLYSYDTGSKD--FLSINMAGGHVEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVD------KQKA-------VEGMAEGGFTQIKC-NSDIFIGGVP-NYDDVKKNSGILKP-FSGSIQKIILNDRTIHVKHDFTWGVNVENAAHPCVG---SPCA----HGGSCRPRKEGYECDCPLGFEGLHCQKAI-----TEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLG--SGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGK-SPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDA 1006 HSP 2 Score: 85.8853 bits (211), Expect = 2.895e-15 Identity = 86/351 (24.50%), Postives = 143/351 (40.74%), Query Frame = 1
Query: 2737 SFAKSYWSGNAENYLSI---NISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS---HIWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAK 3759
S+A + W +YLS ++ P S DG++ Y DT S D+L+I + +VE+R D GSG L+S LT+G H + V R ++ L +D + GM T + IG P + + + F G + KI + T I++ + T W + N C PC +GG C+ +E Y C C G++G+ C++ ++ I +F S++ + + + R R + F A +GLL + D R N FIS+ L L + GV ++ S G+W+ V ++ +
Sbjct: 627 SYAATPWPLEPRHYLSFMEFEVTFRPDSEDGVLLYSYDTG-SKDFLSINMAGGHVEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGV--PNYDDVKKNSGILKPFSGSIQ--KIILNDRT-IHVKHDFT-WGVNVENAAHPC---------------------------------VGSPCAHGGSCR-PRKEGYECDCPLGFEGLHCQKAITEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQ 936
BLAST of Agrin, putative vs. TrEMBL
Match: A0A4D6G2W8 (Agrin (Fragment) OS=Schmidtea mediterranea OX=79327 GN=agrin PE=2 SV=1) HSP 1 Score: 627.091 bits (1616), Expect = 0.000e+0 Identity = 305/310 (98.39%), Postives = 309/310 (99.68%), Query Frame = 1
Query: 1177 HLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRL 2106
HLKIESCKKKISLTVMYKG+CQTKIQSDI SNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICN++PRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHAC+TNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRL
Sbjct: 1 HLKIESCKKKISLTVMYKGRCQTKIQSDIXSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNDKPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACDTNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRL 310 HSP 2 Score: 105.916 bits (263), Expect = 9.155e-21 Identity = 95/323 (29.41%), Postives = 144/323 (44.58%), Query Frame = 1
Query: 490 ISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCP-PYATCV--TNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVK-----CEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESC-KKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSC 1431
++V G ++Q+D S + C +GA+C K A C C C+ ++ VCG+DGI YD+ C + + C Q + V ++ C C CP PY C N C+CP + C +CG D++TY +IC + + +C + + Y G C+ K C IC+ D + C+CP + + VCG+D TY+SECH+++ +C I+L + G C D C N +C + + C QC C DC R PVCG+D Y+N+CFL SC
Sbjct: 13 LTVMYKGRCQTKIQSDIX-SNIQCHYGAKCIKTSNT--VAQCSCDYLDCKNTWRD-PVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNP-------------CSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNDKPRCKEQGKDCPSYQICK--SDLVQAKCECPTCPQ---SGLGESVCGTDGKTYKSECHMRMHACDTNSIALKIANIGTC------DKCKNKKCSFYSICQMNRFLEPQCICP-TDCVYV-RRPVCGSDDRTYENECFLKVKSC 305 HSP 3 Score: 105.916 bits (263), Expect = 9.329e-21 Identity = 98/319 (30.72%), Postives = 155/319 (48.59%), Query Frame = 1
Query: 1426 SCEKQMEMHVINSNFCENNPCSGVKCPDPYQY---CRIDNNGKSTCECPKEPCPKIVQ-PVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPS-YQICKSDL-VQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCD---KCK--NKKCSFYSICQMNRFLEPQCICPTDCVY--VRRPVCGSDDRTYENECFLKVKSCAENRLITAVSE-GPCKLQFIKPDEERCP-EDCPLGKMCR-----NGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNK 2322
SC+K++ + V+ C+ S + Y C +N + C C C + PVCGND + Y++ C L + +C + + +I + C P G CP YQ C+ D ++ CECP P + + VCG D TY+S C++ AC T I N G C+ +CK K C Y IC+ + ++ +C CPT C + VCG+D +TY++EC +++ +C N + ++ G C ++C + C +C+ QC+C C V+ VCG+D YENEC LK K+C +N+
Sbjct: 6 SCKKKISLTVMYKGRCQTKIQSDIXSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPC---SGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYN-GICNDKPRCKEQGKDCPSYQICKSD-LVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACDTNSIALKIANIGTC---------DKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENR 309
BLAST of Agrin, putative vs. TrEMBL
Match: A0A4S2KB71 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011105 PE=4 SV=1) HSP 1 Score: 591.267 bits (1523), Expect = 1.959e-178 Identity = 436/1482 (29.42%), Postives = 670/1482 (45.21%), Query Frame = 1
Query: 1135 PVCGSDDVTYQSECHLKIESC----------KKKISLTVMYKGKC-------QTKIQSDIC-SNIQCHYGAKC--IKTSNTVAQ--------CSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQIC----------------------KSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVR--RPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKC----SKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGC-----------ISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENG--CTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKS------------------------------YWSGNAENYLSINISLVPYSSDG-IIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAI---FLKSRV--QLTIGRS---------HSILVERLNRDSRLVIDGTDSQYGMNPPFL--------TNLDAGF-IFTIGQATGPIINTL--SHTGDVTDGFVGCLSSLKITAG---EETKIYLMN---QQTSWI-----IESSNVKPNC-PHIDFKNSVDVIEDPDKEEQSEKENLKIKDY------CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFP---SESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLN-KNHLEVTCRT---------PIGVTR-----LISMESAL-IGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLN-VNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRF-----QNSDIILN----------NITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE----------------------------NLCIRNSMQHDNPANTNFFRK-------------------YLGKSASKYAAVKQKRD--------------------KSSTKNNIRIKFKTASNDGMXXX-------------XXXXXXXXXXXXVIAIRNGVIEVAINLGGK------------------NGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
PVCG+D V YQ+EC L+ SC + + ++V Y KC QT+ +C N C+YGAKC I +N+ C C +++C N W DPVCG DG Y N+C+L K C Q+ ++ C +NPC C P + C ID G+ C CP + CPK++ PVCG+D TYES C+L TAC+ + +W++Y+G C++EP+C+ G C Y++C K + A C CPTCP+ GLG+ VCGTDG+TY+SECH+R C+ +K+ +IG CD C NK+C +Y+ICQ N EPQCICPTDC YV+ + VCG+D TYE+EC LKV+SCAE R I + EGPCK CP CPLG CRNGQCVC D C S + VCGTDG Y +ECELKR+AC+ K I PS C ++ AD C CN +G+L+ CD +C CK + G CD+C+ GYW +NG C ACSC+ GS S C+ +G+C+C+P +G+ CS+CP G ++ GC ++ L ++ +KS + E ++I + +V DG ++ Y L LK+ ++R+ + G + +L R+ +L + ++ H I V N L +DG + P L T G+ IG+ P I + DGF GCL + + G + I L++ + +WI + P C + +F + +V+ + D+ + + + ++ C +PC NGG+C ++ Y C C PG+QG CE+VV+ I +F +SF+ P ++L++ +TFL GLL F+ P F+ V ++ K ++ V CRT P V R L+ L + +W+ + I K + + L ++ + Q + RL+P + L F + + PI+ GG D NS + + ++ GAIQ++ IN +I N ++ N N++QWQGPPCGP SPC + + C P C CS + + N NS DN + + +G+ +AA + R+ KS T +R++ +TAS DG++++ L+ ++ +IA+R G +E I + + +L+I ++D K HTV V+R+ R L++D +M + + ++ D ++ +GGA + G PWEY F GCI +L+++E +++ A + GPI+ C
Sbjct: 220 PVCGTDGVMYQNECLLRKRSCAGGSWSPYSPSRPVMVSVDYTNKCLNQYHARQTRPSYALCDGNNVCNYGAKCPAINPANSWRHVAPAPFNGCDCQHINCLNEWPDPVCGTDGETYSNECYLRKSMCVAQLPKRILYRGNCASNPCLSHTCRWPGERCEIDETGQPKCVCP-DSCPKVMLPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQEPQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCPTCPEKGLGDQVCGTDGQTYRSECHLRASVCQRQLYGVKVRSIGPCDACNNKECKYYAICQKNADGEPQCICPTDCPYVQGGKTVCGNDGNTYEDECVLKVRSCAEQREIYVIHEGPCKA---------CPNGCPLGYQCRNGQCVCRDACPPTSSLLDAEVCGTDGLLYRSECELKRQACLQGKEINADPSGASCRKRLDSNSEDVVAVRPQADGVQVSQCTCNKVGALSEFCD-HRGRCRCKWYVQGDKCDQCVAGYWGL-QNGHECIACSCHPDGSVSTNCDQTSGQCDCRPGVVGRQCSICPDGGHVTKSGCNVENVTSGYRATESLSLKSEAKSKSDNNTPPPPDSLQTNEIITGRQFSPETTLLIPAPQSFEQPITIELDVVLSGQDGNLLQYRIAPTEQEQMLVKNLKDH--QFRLGISGGRVECSYLNGRIPDRLYVVQAPETIRPNTLHQIRVGVNNNKPWLQLDGVQTTLVTEAPILQPSTAENITQPVGGYKTIVIGRPLFPAIQKTWPPPSARENDGFGGCLLRVNLITGSNKQAQPIDLLSGAGTELAWIGIKRMTSGLSEAPLCSANEEFSSPPEVLHEDDRTKSIQSDGSTVEQLQAPATICTREEPCQNGGICVSTRPNEYDCICQPGWQGQRCEQVVTVIPQFGGDSFIRLPGPKGPQAMNGKKLRVGLTFLVTQVPGLLFFVPPKTRMGP-FLGVYIDPKGYVVVACRTELRALDAKFPDSVGRSSDTVLLQYRIPLQLRRWHVLTIDKRPRAMSLKIDEN-QSQRIRLVPAALRRYLKKWRGLSLPSFDLSSSPIYLGGYD--FHNSPTPPMDIRTGLVGAIQQMNINGADIFLVGPPSPNVEVTQNSLSDKSEYWLNVSQWQGPPCGPAYSPC-RIGELQSVCRPLGTTASCACSTQSQLIRFMREHGTNDANWAETLACQRKQAEINAMRENSQTSDNETPQKPWSQDDVQYDALPRTVESPGTDPKIGQGKDAFAATPETRNCAQLAVRFSGNTIFKYFNLLKSMTDYYMRLQLQTASQDGLILMIGDTLPETKRPGYLQSKLYDQRELTMIALRKGRVEFGIFIVSEITTAASDRRIALITGRTSTNLLQIQSKMELNDGKCHTVNVVRSGRRATLIVDHQMASGEF--SVPSQSMHQDNYVLMGGAMLNITGIPWEYQHNFTGCIGDLFLNEQKVHMLTSAFEIRGPIMSC 1680 HSP 2 Score: 149.443 bits (376), Expect = 3.765e-32 Identity = 139/461 (30.15%), Postives = 205/461 (44.47%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTN----------------------PYPY--CKCPNDKC-DSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRC--DICKFVKCEFNG-ICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQ----------SDICSNI------QCH----YGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINS----NFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIV--QPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGES-VCGTDGKTYKSECHMRMHAC 1878
VCG+DG+ Y + CL++++ C SP+ D +K Q+ Q+ P YA C N P P+ C C + C + +CGTD TY+N C ++K C A Y G C + C C + G CE+ E G C CP+ C K + +PVCGSD TY+S CHL++ +C K + V+Y G+C + Q ++C+ I Q H G++ K VA C C K D VCG DG Y ++C L C++Q+ + S + C N C Y C+ + +G+ C CP + CP + + VCGND TYE C L +C ++ +++I+ G C P CP C++ + CP P S L ++ VCGTDG Y+SEC ++ AC
Sbjct: 221 VCGTDGVMYQNECLLRKRSCAGGSWSPYSPSRPV---MVSVDYTNKCLNQYHARQTRPSYALCDGNNVCNYGAKCPAINPANSWRHVAPAPFNGCDCQHINCLNEWPDPVCGTDGETYSNECYLRKSMCVAQLPKRILYRGNCASNPCLSHTCRWPGERCEIDETGQPK-CVCPDS---CPK-VMLPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQEPQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCPTCPEKGL-GDQVCGTDGQTYRSECHLRASVCQRQLYGVKVRSIGPCDACNNKECKY------YAICQKNADGEPQCICPTD-CPYVQGGKTVCGNDGNTYEDECVLKVRSCAEQREIYVIHEGPCKACP------NGCPLGYQCRNGQCVCRDACP--PTSSLLDAEVCGTDGLLYRSECELKRQAC 657 HSP 3 Score: 97.4413 bits (241), Expect = 3.079e-16 Identity = 89/325 (27.38%), Postives = 143/325 (44.00%), Query Frame = 1
Query: 538 DPCSKLNCKF-GAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRK-EAQQDGISKSNEQFCQHQSCPPYATC------------VTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRF-IEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPN--PDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSC-DYLDCKNTWRDP-VCGNDGIIYDNKCFLMKMSCEKQMEMHV 1455
+PC C++ G +C+ P VC S + + VCGSDG Y+SHC ++ C + I V + C ++ + Q G+ + C PP +C CP Q+CGTD TY + C ++ C+ + ++ + +G CD C +C++ IC+ DG C CP P K VCG+D TY+ EC LK+ SC ++ + V+++G C+ C N C G +C QC C D ++ D VCG DG++Y ++C L + +C + E++
Sbjct: 363 NPCLSHTCRWPGERCEIDETGQPKCVCPDSCPKVM-----LPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQEPQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCPTCPEKGLGDQVCGTDGQTYRSECHLRASVCQRQLYGVKVRSIGPCDACNNKECKYYAICQKNADGEPQ-CICPTDCPYVQGGKT----VCGNDGNTYEDECVLKVRSCAEQREIYVIHEGPCKA------CPN-GCPLGYQCRN-----GQCVCRDACPPTSSLLDAEVCGTDGLLYRSECELKRQACLQGKEINA 665 HSP 4 Score: 90.8929 bits (224), Expect = 2.562e-14 Identity = 99/369 (26.83%), Postives = 148/369 (40.11%), Query Frame = 1
Query: 1558 CPKEPCPKIVQPVCGNDKVTYESICNLTRTACL--------TKKPLWII--YNGICNEEPRCKEQGKDCPSYQICKSDLV---QAKC----------ECPTCPQSG-----------LGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTC--DKCKNKKCSFYS-ICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRN--------------------------GQCVCTDKCSKVKL--RVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKT 2469
C PC ++PVCG D V Y++ C L + +C +P+ + Y C + ++ PSY +C + V AKC P +G + VCGTDG+TY +EC++R C + +I G C + C + C + C+++ +P+C+CP C V PVCGSD TYE+ C L++ +C + R I V G C + P + C ++C CVC C + L +VCGTDG Y +EC L+ C S+ C D N + C +I N + +P QCIC T
Sbjct: 209 CDPIPCSLELRPVCGTDGVMYQNECLLRKRSCAGGSWSPYSPSRPVMVSVDYTNKCLNQYHARQTR---PSYALCDGNNVCNYGAKCPAINPANSWRHVAPAPFNGCDCQHINCLNEWPDPVCGTDGETYSNECYLRKSMC-VAQLPKRILYRGNCASNPCLSHTCRWPGERCEIDETGQPKCVCPDSCPKVMLPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQE---PQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCP-TCPEKGLGDQVCGTDGQTYRSECHLRASVCQRQLYGVKVRSIGPC-------DACNNKECKYYAICQKNADGEP---QCICPT 559
BLAST of Agrin, putative vs. TrEMBL
Match: A0A4E0RI86 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_002192 PE=4 SV=1) HSP 1 Score: 564.688 bits (1454), Expect = 7.908e-168 Identity = 418/1394 (29.99%), Postives = 625/1394 (44.84%), Query Frame = 1
Query: 532 NDDPCSKLNCKFGAQCQKWPG---NPPTAVCHCSNT---------QCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQ----------VSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESC----KKKISLTVMYKGKC-----QTKIQSD--IC-SNIQCHYGAKC-------------IKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICK------------------------SDLVQ--AKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSK---VKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCI---------------------SHNYI----ADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENG--CTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC--KENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDG-IIFYHSDTDRSSDYLTIVLKNR-----YVEYRVDL------GSGAIF-LKSRVQLTIGRSHSI---------LVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTD---GFVGCLSSL-------------KITAGEETKIYLM--NQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTH-------KPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSES---LQLRQRLKLEITFLPLANNGLLSFISD----GHDRNPAFISVTLN-KNHLEVTCR------------TPIGVTRLISMESALIG---KWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNI--------------TQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCS 4146
+ PC ++ C FGA C+ WP VC C Q+G VC G YD+ C + C Q ++ V+ +C + A++ + K+ + F T P P K + D + +VG+ D ++ED + C D L N PVCG+D VTY +E L+ SC K + + Y C + K Q D +C N C +G C +S +CSC++++C DPVC DG Y N+CFL + CE Q+ V+ C +NPC G C + C++D G+ C CP +PCP +V PVCG+D+VTY+S C+L RTAC+ + ++I+Y G C+EEP C+ G C Y+IC S VQ A+C CPTCP+ G+G+ VCGTDG+TY+SECH+R AC+ LK+ G+CD CK K C +++IC++N EP+CICPTDC+YVR+PVCG+D + YENECF+KV +CAE R I EGPC CP CPLG C G+CVC D C K ++ VCG+DG Y +ECELKR+AC+ +RV PS C NY+ ADE SC CN++G+L+ CD +C CK G+ G+ CD+C+ YW +NG C ACSC+ G+ S C+ TG+C+C+P G+ CS+CP ++ +GC E Y++++ F D+ + ++ + L + +G ++FY R L R V R+DL SG I +++ QL +GR H++ ++ V D + ++ + + IG+ +++T + +T GF GCL L + +G+ T++ + NQ S + E+ P C D N V I+ D++++ E L ++H +PC NGG C+ +S Y+C CLPG+QG C++V + + +F SFV P S R+RL +EI FL GL+ I N FIS+ ++ K L CR P RLI+ ++ +W+ +++ K + + + L++ P+ + R +P F ++ + ++LT F + PI+ GG + V ++ GA+QK+ IN + I N I TQWQG PCGP SPC PE C P+ C CS
Sbjct: 175 TERPCDRVRCHFGAICETWPSPSNEKQVGVCVCPQIDVLHRRGLCHIQSG----PVCAHTGRLYDNDCALTLAICVQQSILEKIELPRTAAGVALRELCTQMAAERLKVQKTEKSFELENLATRGNVIATVPITTTARPTPKQKENFEDKPQQDAAVPYGL----------------PFVGQQDF-------------VLEDNVNAASDC---DGLPCPNTLKPVCGTDGVTYLNEYLLRERSCLVTSSGKPPIRIHYVSHCISQKLENKNQPDALLCPGNNVCQFGGLCSIRRTASSHEAISFTSSALFDRCSCEHINCLGRGSDPVCAADGQTYSNECFLRRRICETQIPRAVLYRGNCASNPCLGYTCRWAGEQCQVDETGQPKCVCP-QPCPNVVSPVCGSDRVTYDSHCHLERTACMKMRDIFILYAGQCSEEPHCELLGLQCQGYEICSRLPTPHVHVPMSRYSNPWSNVISSSQPVQLVARCLCPTCPERGIGDQVCGTDGQTYRSECHLRASACQRQIRQLKVRARGSCDACKTKDCPYHAICRLNSLGEPECICPTDCLYVRKPVCGTDGKMYENECFMKVAACAEQREIHVAQEGPCAT---------CPFGCPLGYQCLKGECVCRDACPKPTALEAEVCGSDGQIYPSECELKRQACLRQTNVRVDPSGARCRDLANRIQTNGTLSGQDGFQAGGQNYLVQPQADENVPASCSCNTVGALSEHCD-YRGRCHCKWGVRGERCDQCMPNYWGI-QNGHECIACSCHPDGALSTTCDAMTGQCHCRPGIFGRQCSICPNDGRLTKNGCVSPEGIPYDDRLRVSGRRFSADTTLLVRAPFPLKEPFAVQLILQATAPNGSLLFYQLAPSRQEQMLLENPTGRQFFLDIVNGRIDLRYVSNGPSGRILSVQTSQQLQLGRQHTVRAGVTHGRPWIKLDTGPETTVTDIEERKHEASLSSSLSSGGHGTIVIGRHV--LVSTAQYVQPITKGSVGFSGCLLQLVLRSESTMQEQNVDLISGDPTELAWIGVNQLPSGLAEA----PLCQSTD-NNPVSSIDQIDRDDKQETVQLDTVSQDKSHSKNPCDRQPCGNGGSCELTSNVGYTCICLPGWQGHHCDQVATIVPQFHRGSFVRLPGPSGRQAMQRKRLHIEIVFLLTDFEGLILLIPPSMRFAKSNNGPFISIYVDAKGFLFTHCRVGHPMEDGQDVLAPNSRARLITFRYSVPAQRNQWHTLIVEKRTRALIIQLDNGPKE-RVRTVPTNFRRLSRYIRQQLTAFDLSESPIYVGGFS--PTDQSVPPVPIRNGMMGAVQKLSINGAEVVLAGP-IASNTIGPSAAEPTELWFKVTQWQGKPCGPNYSPCLPDKPES-ICRPQGALATCACS 1508
BLAST of Agrin, putative vs. TrEMBL
Match: A0A4S2KBC1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011105 PE=4 SV=1) HSP 1 Score: 548.125 bits (1411), Expect = 1.954e-164 Identity = 410/1418 (28.91%), Postives = 640/1418 (45.13%), Query Frame = 1
Query: 1258 DICSNIQCHYGAKCIKTSN-TVAQCSCDYLDC----KNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQIC----------------------KSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVR--RPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKC----SKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGC-----------ISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENG--CTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKS---------------YWSGNA---------------ENYLSINISLVPYSSDG-IIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAI---FLKSRV--QLTIGRS---------HSILVERLNRDSRLVIDGTDSQYGMNPPFL--------TNLDAGF-IFTIGQATGPIINTL--SHTGDVTDGFVGCLSSLKITAG---EETKIYLMN---QQTSWI-----IESSNVKPNC-PHIDFKNSVDVIEDPDKEEQSEKENLKIKDY------CQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFP---SESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLN-KNHLEVTCRT---------PIGVTR-----LISMESAL-IGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLN-VNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRF-----QNSDIILN----------NITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHE----------------------------NLCIRNSMQHDNPANTNFFRK-------------------YLGKSASKYAAVKQKRD--------------------KSSTKNNIRIKFKTASNDGMXXX-------------XXXXXXXXXXXXVIAIRNGVIEVAINLGGK------------------NGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
D C+N +C + A C+ + + C C Y C +T + P+CG+DG Y + C L + +C M++ + C +NPC C P + C ID G+ C CP + CPK++ PVCG+D TYES C+L TAC+ + +W++Y+G C++EP+C+ G C Y++C K + A C CPTCP+ GLG+ VCGTDG+TY+SECH+R C+ +K+ +IG CD C NK+C +Y+ICQ N EPQCICPTDC YV+ + VCG+D TYE+EC LKV+SCAE R I + EGPCK CP CPLG CRNGQCVC D C S + VCGTDG Y +ECELKR+AC+ K I PS C ++ AD C CN +G+L+ CD +C CK + G CD+C+ GYW +NG C ACSC+ GS S C+ +G+C+C+P +G+ CS+CP G ++ GC ++ L ++ +KS +G E ++I + +V DG ++ Y L LK+ ++R+ + G + +L R+ +L + ++ H I V N L +DG + P L T G+ IG+ P I + DGF GCL + + G + I L++ + +WI + P C + +F + +V+ + D+ + + + ++ C +PC NGG+C ++ Y C C PG+QG CE+VV+ I +F +SF+ P ++L++ +TFL GLL F+ P F+ V ++ K ++ V CRT P V R L+ L + +W+ + I K + + L ++ + Q + RL+P + L F + + PI+ GG D NS + + ++ GAIQ++ IN +I N ++ N N++QWQGPPCGP SPC + + C P C CS + + N NS DN + + +G+ +AA + R+ KS T +R++ +TAS DG++++ L+ ++ +IA+R G +E I + + +L+I ++D K HTV V+R+ R L++D +M + + ++ D ++ +GGA + G PWEY F GCI +L+++E +++ A + GPI+ C
Sbjct: 66 DPCTNYRCAFQAWCVPSKDFKRPTCVC-YNTCYDVGDSTDKGPICGSDGREYSSVCQLRREACSMMMDIEIKYRGKCASNPCLSHTCRWPGERCEIDETGQPKCVCP-DSCPKVMLPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQEPQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCPTCPEKGLGDQVCGTDGQTYRSECHLRASVCQRQLYGVKVRSIGPCDACNNKECKYYAICQKNADGEPQCICPTDCPYVQGGKTVCGNDGNTYEDECVLKVRSCAEQREIYVIHEGPCKA---------CPNGCPLGYQCRNGQCVCRDACPPTSSLLDAEVCGTDGLLYRSECELKRQACLQGKEINADPSGASCRKRLDSNSEDVVAVRPQADGVQVSQCTCNKVGALSEFCD-HRGRCRCKWYVQGDKCDQCVAGYWGL-QNGHECIACSCHPDGSVSTNCDQTSGQCDCRPGVVGRQCSICPDGGHVTKSGCNVENVTSGYRATESLSLKSEAKSKSDNNTPPPPDSLQTNEIITGRQFSPETTLLIPAPQSFEQPITIELDVVLSGQDGNLLQYRIAPTEQEQMLVKNLKDH--QFRLGISGGRVECSYLNGRIPDRLYVVQAPETIRPNTLHQIRVGVNNNKPWLQLDGVQTTLVTEAPILQPSTAENITQPVGGYKTIVIGRPLFPAIQKTWPPPSARENDGFGGCLLRVNLITGSNKQAQPIDLLSGAGTELAWIGIKRMTSGLSEAPLCSANEEFSSPPEVLHEDDRTKSIQSDGSTVEQLQAPATICTREEPCQNGGICVSTRPNEYDCICQPGWQGQRCEQVVTVIPQFGGDSFIRLPGPKGPQAMNGKKLRVGLTFLVTQVPGLLFFVPPKTRMGP-FLGVYIDPKGYVVVACRTELRALDAKFPDSVGRSSDTVLLQYRIPLQLRRWHVLTIDKRPRAMSLKIDEN-QSQRIRLVPAALRRYLKKWRGLSLPSFDLSSSPIYLGGYD--FHNSPTPPMDIRTGLVGAIQQMNINGADIFLVGPPSPNVEVTQNSLSDKSEYWLNVSQWQGPPCGPAYSPC-RIGELQSVCRPLGTTASCACSTQSQLIRFMREHGTNDANWAETLACQRKQAEINAMRENSQTSDNETPQKPWSQDDVQYDALPRTVESPGTDPKIGQGKDAFAATPETRNCAQLAVRFSGNTIFKYFNLLKSMTDYYMRLQLQTASQDGLILMIGDTLPETKRPGYLQSKLYDQRELTMIALRKGRVEFGIFIVSEITTAASDRRIALITGRTSTNLLQIQSKMELNDGKCHTVNVVRSGRRATLIVDHQMASGEF--SVPSQSMHQDNYVLMGGAMLNITGIPWEYQHNFTGCIGDLFLNEQKVHMLTSAFEIRGPIMSC 1461 HSP 2 Score: 137.117 bits (344), Expect = 2.059e-28 Identity = 119/372 (31.99%), Postives = 179/372 (48.12%), Query Frame = 1
Query: 865 PYCKCPN---DKCDSSS-SQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRC--DICKFVKCEFNG-ICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQ----------SDICSNI------QCH----YGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINS----NFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIV--QPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGES-VCGTDGKTYKSECHMRMHAC 1878
P C C N D DS+ ICG+D Y+++C++++ +C IE +Y G+C + C C + G CE+ E G C CP+ C K + +PVCGSD TY+S CHL++ +C K + V+Y G+C + Q ++C+ I Q H G++ K VA C C K D VCG DG Y ++C L C++Q+ + S + C N C Y C+ + +G+ C CP + CP + + VCGND TYE C L +C ++ +++I+ G C P CP C++ + CP P S L ++ VCGTDG Y+SEC ++ AC
Sbjct: 88 PTCVCYNTCYDVGDSTDKGPICGSDGREYSSVCQLRREACSMMMDIEIKYRGKCASNPCLSHTCRWPGERCEIDETGQPK-CVCPDS---CPK-VMLPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQEPQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCPTCPEKGLG-DQVCGTDGQTYRSECHLRASVCQRQLYGVKVRSIGPCDACNNKECKY------YAICQKNADGEPQCICPTD-CPYVQGGKTVCGNDGNTYEDECVLKVRSCAEQREIYVIHEGPCKACP------NGCPLGYQCRNGQCVCRDACP--PTSSLLDAEVCGTDGLLYRSECELKRQAC 438 HSP 3 Score: 126.716 bits (317), Expect = 3.043e-25 Identity = 121/413 (29.30%), Postives = 176/413 (42.62%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPG-NPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCP-PYATCVTNP--YPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCD---ICKFV--KCEFNGICEMME--------------------DGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQC----SCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPK----IVQPVCGNDKVTYESICNLTRTACLTKK 1665
DPC+ C F A C PT VC+ + + +CGSDG Y S C ++R+ C + I++ C C +C P C + P C CP D C +CG+D TY + C ++ +C R I Y G+C C+ + C+ +C ++ + C CP E K + VCG+D TY+SECHL+ C+++ +Y K ++ D C+N +C Y A C K ++ QC C Y+ T VCGNDG Y+++C L SC +Q E++VI+ C+ P CP YQ CR NG+ C ++ CP + VCG D + Y S C L R ACL K
Sbjct: 66 DPCTNYRCAFQAWCVPSKDFKRPTCVCYNTCYDVGDSTDKGPICGSDGREYSSVCQLRREACSMMMDIEIKYRGKCASNP-------------CLSHTCRWPGERCEIDETGQPKCVCP-DSCPKVMLPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQEPQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCPTCPE---KGLGDQVCGTDGQTYRSECHLRASVCQRQ-----LYGVKVRSIGPCDACNNKECKYYAICQKNADGEPQCICPTDCPYVQGGKT----VCGNDGNTYEDECVLKVRSCAEQREIYVIHEGPCKACPNG---CPLGYQ-CR---NGQCVC---RDACPPTSSLLDAEVCGTDGLLYRSECELKRQACLQGK 442 HSP 4 Score: 97.0561 bits (240), Expect = 3.864e-16 Identity = 89/325 (27.38%), Postives = 143/325 (44.00%), Query Frame = 1
Query: 538 DPCSKLNCKF-GAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRK-EAQQDGISKSNEQFCQHQSCPPYATC------------VTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRF-IEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPN--PDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSC-DYLDCKNTWRDP-VCGNDGIIYDNKCFLMKMSCEKQMEMHV 1455
+PC C++ G +C+ P VC S + + VCGSDG Y+SHC ++ C + I V + C ++ + Q G+ + C PP +C CP Q+CGTD TY + C ++ C+ + ++ + +G CD C +C++ IC+ DG C CP P K VCG+D TY+ EC LK+ SC ++ + V+++G C+ C N C G +C QC C D ++ D VCG DG++Y ++C L + +C + E++
Sbjct: 144 NPCLSHTCRWPGERCEIDETGQPKCVCPDSCPKVM-----LPVCGSDGTTYESHCHLELTACMKMRQIWVVYSGQCSQEPQCQAIGLHCQGYEVCTRIKAPPVYQIHSQQYSGSQSSKFQPMVAHCVCPTCPEKGLGDQVCGTDGQTYRSECHLRASVCQRQLYGVKVRSIGPCDACNNKECKYYAICQKNADGEPQ-CICPTDCPYVQGGKT----VCGNDGNTYEDECVLKVRSCAEQREIYVIHEGPCKA------CPN-GCPLGYQCRN-----GQCVCRDACPPTSSLLDAEVCGTDGLLYRSECELKRQACLQGKEINA 446 HSP 5 Score: 77.7962 bits (190), Expect = 2.875e-10 Identity = 53/154 (34.42%), Postives = 74/154 (48.05%), Query Frame = 1
Query: 1915 GTCDKCKNKKCSFYSICQMNR-FLEPQCICPTDCVYV-----RRPVCGSDDRTYENECFLKVKSCA-----ENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQ--CVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVA 2337
G D C N +C+F + C ++ F P C+C C V + P+CGSD R Y + C L+ ++C+ E + + PC + ERC D GQ CVC D C KV L VCG+DG YE+ C L+ AC+ + I V
Sbjct: 63 GIKDPCTNYRCAFQAWCVPSKDFKRPTCVCYNTCYDVGDSTDKGPICGSDGREYSSVCQLRREACSMMMDIEIKYRGKCASNPCLSHTCRWPGERCEID-------ETGQPKCVCPDSCPKVMLPVCGSDGTTYESHCHLELTACMKMRQIWVV 209
BLAST of Agrin, putative vs. TrEMBL
Match: A0A5K4EU74 (Agrin, putative OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 545.428 bits (1404), Expect = 1.659e-160 Identity = 383/1188 (32.24%), Postives = 569/1188 (47.90%), Query Frame = 1
Query: 1135 PVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQ-----SDICSNIQ-CHYGAKCI--------------KTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQIC---KSDLVQA----------------------KCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKC---SKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGC--------------ISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENG-CTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCK-----------ENSIYENKVDAVELG----FLKDSFAKSYWSGNAE------NYLSINISLVPYSSD---------------GIIFYHS----------DTDRSSDYLTIVLKNRYVEYRVDLGS---GAIFLKSRVQLTIGRSHSIL-----------VERLNRDSR--LVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTD--GFVGCLSSLKITAGEETKIY------LMNQQTSWI-IE-------------SSNVKP-NCPHIDFKNSVDVIEDPD--KEEQSEKENLKIKDYCQT------HKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQL---RQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLN-KNHLEVTCRTPIG------VTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDI--ILN----------------NITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCS 4146
P+C S+ Y ++C LK SC+K + + C + +Q +++C N C YG KC K + C C++++C N W DPVC +DG Y N+CFL + CE Q ++ C +NPC KC + C++D NG++ C CP EPCP V PVCG+D VTY+SIC+L RTAC + + +IY+G C+E C + C Y+IC K+ +V +C CPTCP+ GLG VCG+DG+TY+SECH+R AC+ +S L + + G CD C+ K+C +Y+ICQ++ F EPQCICPTDC+YVR+PVCG+D +TYENECFLKVKSCA+ R + EG C+ C CPLG CRNGQCVC D C +L VCGTDG Y +ECELKR+ACI I V + + C S N AD SC CN +G+L+ ECD C CK G+ G CD+C+ YW N C CSC+ GS CN TG+CNC+ +G+ CS+CP G ++ +GC E++ + A EL F+ K N E + + +P+ D G++ ++ D ++ + N V + GS + + S+ LT H+I +++ +R + ++ D+ N P +L+ I + G I+ + D+ D GF GCL+ + I AG TK + +N + +W+ IE SS++K + P D NS + P + +Q ++ + I QT K C N G+ S+ Y+C C PG+QG CE+ V+ I +F N F+ + + R++L +E+ FL +GLL FI N FI+ L+ +N+L V CRT G + +L E + +WN ++I K K + + +N + + + LIP FL++ + + ++LT F + + PI+ GG S N + ++ FYGAIQ+I IN+ + + ++N N++QWQG PCGP SPC P C P + C C+
Sbjct: 402 PICDSEGTIYLNDCLLKKYSCRKIGTGELPRIISCNSTLQKYKPKAELCGNGNLCLYGGKCYDPLDHYYRSKMIMKKILPLYSNCICEHINCLNDWPDPVCADDGETYTNQCFLKRAICETQSLKRILYRGNCASNPCLHHKCRWQGEKCQVDVNGQAKCTCP-EPCPSAVSPVCGSDGVTYDSICHLERTACQKMREIRVIYSGECSEPKDCILLNQPCQGYEICSRIKNPMVYTSINRKLDASVYDLSNSFEDTIPECICPTCPEYGLGGQVCGSDGQTYRSECHLRSSACQRHSTDLTVKSRGKCDACQTKQCKYYAICQLSAFGEPQCICPTDCLYVRKPVCGTDGKTYENECFLKVKSCADQREVHVAQEGYCR---------PCTAGCPLGFQCRNGQCVCRDACPPKHPTELDVCGTDGKLYSSECELKRQACIHQTNIDVDLTGVRCRSKHPTVLQDTNMKTSGNIQADGVRLSSCTCNKLGALSEECD-YLGNCRCKWGVGGLKCDQCLANYWGIQNNHECIPCSCHPLGSTDTTCNQATGQCNCRTGVVGRQCSMCPDGSQVTENGCSVKRQSEADIKFESNEQQYSPGATELNKTNTFISQLPDKLTAENNKEEDGRLFTETTTLLVRIPFHIDQPTEIQMILGLDKGNGMLLHYRAPPSEQEQMISRDTQDHQFSMAISNWRVVLKYIDGSVPNRILLVYSKHNLTRNTKHNIQAGIISGTPWIKIDKFSRTTNQDVIEKWADTHQSDNQPTAGSLETIIIGRQIKIGGQSISDMKERADLHDDYGFSGCLNKMIINAGSPTKHFSLDLLEAINTKLAWLGIEPNPSTGHSEAPLCSSSIKALSSPTNDISNSNTIENIPPLLQSDQVQRHDNIINQNVQTLSSHIQTKKCENYGIESIGSDGKYTCICQPGWQGGVCEQAVTIIPQFSGNGFIRLAGPTGKYAIKRRKLHIELIFLVTQPSGLLFFIPPKSPHNGPFIAAYLDEQNYLNVVCRTGKGKLVNENLIQLRYNEPVQLNQWNTLLIDKLQKVLRVKVNGNKRQ-RVSLIPSRFLHIQNHLLKELTNFDLSSSPIYLGGYG-FYDQSILNYIPIRHGFYGAIQRININEAEVVLAGPSLADMMNTPEEQGKLPHLFEKWANVSQWQGLPCGPQYSPCQLNQP-YSICRPNIQHATCACT 1575 HSP 2 Score: 131.339 bits (329), Expect = 1.510e-26 Identity = 119/377 (31.56%), Postives = 157/377 (41.64%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYA---TCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRC----------------DICKFVK-------------CEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKK-KISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGI 1692
VC DG Y + C +KR C Q K G SN C H C N C CP + C S+ S +CG+D +TY +IC +++ +C+ R I Y G C +IC +K + ED + C CP E + VCGSD TY+SECHL+ +C++ LTV +GKC D C QC Y A C ++ QC C DC R PVCG DG Y+N+CFL SC Q E+HV +C PC+ CP +Q CR NG+ C P VCG D Y S C L R AC+ + + + G+
Sbjct: 501 VCADDGETYTNQCFLKRAICETQ-----------SLKRILYRGNCASNP--CLHHKCRWQGEKCQVDVNGQAKCTCP-EPCPSAVSPVCGSDGVTYDSICHLERTACQKMREIRVIYSGECSEPKDCILLNQPCQGYEICSRIKNPMVYTSINRKLDASVYDLSNSFEDTIPE-CICPTCPEY---GLGGQVCGSDGQTYRSECHLRSSACQRHSTDLTVKSRGKC------DACQTKQCKYYAICQLSAFGEPQCICP-TDCLYV-RKPVCGTDGKTYENECFLKVKSCADQREVHVAQEGYCR--PCTA-GCPLGFQ-CR---NGQCVCRDACPPKHPTELDVCGTDGKLYSSECELKRQACIHQTNIDVDLTGV 844 HSP 3 Score: 68.5514 bits (166), Expect = 1.741e-7 Identity = 63/199 (31.66%), Postives = 82/199 (41.21%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPY--PYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKG-KCQTK 1248
VCGSDG Y R EC L+ S C R S+ CQ + C YA C + + P C CP D C +CGTD TY N C +K SC R + G C C C C + C +P EL VCG+D Y SEC LK ++C + ++ V G +C++K
Sbjct: 674 VCGSDGQTY-------RSECHLRSS-------ACQRHSTDLTVKSRGKCDACQTKQCKYYAICQLSAFGEPQCICPTD-CLYVRKPVCGTDGKTYENECFLKVKSCADQREVHVAQEGYCRPCT-AGCPLGFQCRNGQCVCRDACPPKHPTEL-------DVCGTDGKLYSSECELKRQACIHQTNIDVDLTGVRCRSK 849 HSP 4 Score: 65.4698 bits (158), Expect = 1.736e-6 Identity = 36/145 (24.83%), Postives = 65/145 (44.83%), Query Frame = 1
Query: 4378 VIAIRNGVIEVAINLGGK--------------------NGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
+I +RNG +E+ +NL N ++ I ++D +WH +Q + N + L +D+ M + DY+T ++LGG + G +Y F GCI+++ + E ++ +A + GPI RC
Sbjct: 1729 LIVLRNGRVELLLNLVTSRKKKDQSWDVNNNDNGNPEYNRLMPIQARHKVNDGEWHMIQAMGNKQRATLFVDNHMVSGEFAGVADYDTPPVRD-VYLGGTIFNIVGLSADYQQNFTGCIADMLIQEKIIPILSEATEIHGPIERC 1872
BLAST of Agrin, putative vs. Ensembl Cavefish
Match: agrn (agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]) HSP 1 Score: 254.218 bits (648), Expect = 1.318e-67 Identity = 249/892 (27.91%), Postives = 380/892 (42.60%), Query Frame = 1
Query: 202 SDLIIVGRVLNLANSEVI-------VSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQ------NNFFELNYA--KEFGNGLFEKRISVFLN-GATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQ-ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ---MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAE------NRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKAC-------------IDNKTIRVAPSVIGCISHNYIADEKNKRS------------CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
+++++ G V + N + + V V R LKG ++N + N +++ C + +G+ I ++ + N LN + + L E + V G + Q D C C FGA C++ P +P C C C VCGSD Y + C +++ +C +Q+ I+V C K+ C +C +TC+ + C CP C+ Q +CG+D + Y + CE+ +C + I Q+ G CD C+ ++ N C + + + P + C + P+C SD +Y SEC ++ + K+ L ++ G CQ Q C +C + A C+ N A+CSCD + C T++ P+CG DG Y N C C Q + V C+ N PC C D C + NG++ CECP + CP+ PVCGND TY S C++ C +K + I + G C+ + +C IC D +C CPT G + VCG+DG TY +EC + + AC T + L++ G C+K +IC N ++E C+C D V V +C N + V AE + A + C+ DE+ + +CVC C VCG+DG Y++ECELK+ C ++K + + + C Y E K + C CN GS CDP++ QC CK G+ G CDRC G+WNF TEN GCT C+C+ GS C TG C+CK G C VC G + +GC + +
Sbjct: 37 ANIVLTGTVEEIMNMDPVHNTYSCKVRVWRYLKGKTMVNGEVLLDGGNK---VMIGGFGDPGICDNQVATGDTRIFFVNLAPEYMWPAHKNELMLNSSLMRITLRNLEEVELCVEDKPGHITHQPPPPDGCRGKLCGFGAVCERDPADPSKGDCLCKKVPCPL--VVAPVCGSDSSTYTNECELEKAQCSMQRRIKVMRKGPCALKDP------------CNDVTCSYGSTCIQSSDGLSAKCMCPL-SCERVPKQVVCGSDGLDYPSECELNMKACSTQKNIRLQHSGSCDPCRDIQSGLNFTC--LVNAQTRKMETSAPPDSCPPGAE-PLCASDGRSYSSECQMQRTAVLKERHLKKVHSGPCQP--QGKECKE-ECRFSAVCL-LENGRARCSCDPIHCDGTYK-PLCGKDGHTYPNDCERRNAECRTQTHIPVKQQGTCDLNAPSPCLKKIC-DFGGTCVV-KNGEAVCECP-DACPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPCD------KACTNCSFGAIC--DAQTGRCVCPT-ECVGSRQPVCGSDGTTYPNECMLNVKAC-TKQLELRVVAQGECNK--------ITICSKGCWNLYVE-MCVCVFDQVCVYMCIC--------NVLIVCVHVSAECGSGGSGSGVEACEQERCRKYGGSWDEDT-----------EDDRCVCDFTCQN----VCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSETVYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGGLRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGLCSCKTGVKGMKCDVCSDGSKMPANGCHKGA 857 HSP 2 Score: 146.362 bits (368), Expect = 1.281e-34 Identity = 166/745 (22.28%), Postives = 300/745 (40.27%), Query Frame = 1
Query: 2590 TGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP----NCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYC-QTHKPC-----LNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESL---QLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
G C+ +P G C +S + + ++ E ++ ++ KSY + Y ++ I++ +SD GI+ Y+S + D+L++ L VE + D GSG + SRVQ+ G+ H ++V R R++ L +D G +P L+ IG ++ + V+ G VGC+ L + +Y + + ++ + V C KN + + N C +H PC C E Y C+C G +G C +V ++I F S++ L L+Q++ + + L +NG++ + D FIS+ L LE G + S E + WN V + +A + +N+N G V + +K + ++ GG + + + LK F GAIQKI + I + + ++++ + PC G CVP ++ C C +++ ++ A + + G++ +Y K +K+ N + KT + G+++ K +AI +G +++ +LG K VLR T ++ N+W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GC+ ++ VD + +LV DA N IL C
Sbjct: 1290 AGPCDSQPCGHGGTCEEIRDDFSCTCPAGRGGAVCEKMINY----YIPSFGGKSYLAFPTMRAYHTVRIAMEFRASDMSGILLYNSQNGKK-DFLSLALVGGKVELKFDTGSGICSVVSRVQIKPGQWHHLVVNRNRRNAALSVDNEPPVEGQSPTGTDGLNLETHLYIGGVPEDMVQDVKERTSVSAGLVGCIRQLDVN----NMVYNLQENGGNVLFGTAVGECGNNPCSPNPCKNGASCHTKGAEMFHCKCVNAFSGPTCADSHNPCEPNPCHPSSQCAILPEGGYKCECPMGREGKHCRKVSEKKGAYIPYFDGESYLELKGLHLYNHDLKQKVSMTVVLLANDSNGMIFYNGQKTDGKGDFISLALIGGILEFRYDLGKGPAVIRSKEKIKLNVWNTVNLERAQRKGEINVN-------------GKDPVRGESPNQHNDLNLK-ESLYVGGAPDFSKVARAAS--LKSGFKGAIQKITVMGTQILKPENALHSSDVSMYSHHPCS------GDVCHNGGECVPMLDSYECVCPYGFTGAQCQHAT-YEKAAGESEAIAFDGRTFIEYHNGVTKSEKALLVNKFELSIKTEATHGLILWSGKGVERSDYI-ALAIVDGRVQMTYDLGSKPVVLRST--VRVNTNRWIRIKASRALRDGSLQVGNE---AAVTGSSPLAATQLDTDGALWLGGLEELSVARRLPKAYSTGFMGCVKDVIVDGVDLHLVEDAL-NSPKILHC 1995 HSP 3 Score: 104.375 bits (259), Expect = 8.141e-22 Identity = 168/634 (26.50%), Postives = 227/634 (35.80%), Query Frame = 1
Query: 550 KLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVT-NPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKC-QTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEP--------RCKEQGKDCPSYQICKSDLVQA----------KCEC-------PTC-PQSGLGESVCGTDGKTYKSECHMRMHACET---NSIALKIANIGTCDKCK-NKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKV---KSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVC----TDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
K C+F A C G A C C C K + CG DG Y + C + EC Q I V QQ + C + C TCV N C+CP D C S +CG D TY++ C M+ + C + I Q+ G CD C F IC D C CP C + Q PVCGSD TY +EC L +++C K++ L V+ +G+C + I S C N+ C+ V C C+ L I+ C + C + + CE C + + E + C Q VCG+D TY+S C L +T C + L + G C +P C E Y C+ A KC C TC P SG G G +R CE N + N C C + S C+ L C C T ++ VC + N C KSC E + + S C E+ NGQ C D K K +VCG+DG Y ++C+L+ AC +K I V
Sbjct: 389 KEECRFSAVCLLENGR---ARCSCDPIHCDGTYKPL--CGKDGHTYPNDCERRNAECRTQTHIPVK----------QQGTCDLNAPSPCLKKICDFGGTCVVKNGEAVCECP-DACPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPCDK-ACTNCSFGAIC----DAQTGRCVCPTE---CVGSRQ-PVCGSDGTTYPNECMLNVKACTKQLELRVVAQGECNKITICSKGCWNLYVEM-CVCVFDQVCVYMCICNVL---------------IV----CVHVSAECGSGGSGSGVEA--CEQERCR-----------KYGGSWDEDTEDDRCVCDFTCQNVCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSET-----VYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGG--------LRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGL---CSCKTGVKGMKCDVCSDGSKMPANGCHKGASAPKSCTELKCLFGAS---------------CVEE--------NGQAHCECPSPDCDQKNKTKVCGSDGVTYADQCQLRTIACRQDKEIIV 925 HSP 4 Score: 80.4925 bits (197), Expect = 1.489e-14 Identity = 123/507 (24.26%), Postives = 173/507 (34.12%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKAN---RFIEQQYVGRCDIC---------KFVKCEFNGICEMM-----------------------------------------EDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQC------------------------------HYGAKCIKTSNTVAQCSC----DYLDCKN------TWRDPV-----------CGNDGIIYDN----------KCFLMKMSCEKQMEMHVINSNFCENNP-----CSGVKCPDPYQYCRIDNNGKSTCECPKEPC-PKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNE 1701
PC K C FG C G AVC C + Q + VCG+DG Y S C M+ C LQK I + C ++ C + S + C CP + C S +CG+D TY N C + +C R + Q + IC + C F+ +C M ED C C D C VCGSD TY SEC LK C+K++ L + +G C++K S + + + C HYG+ T QCSC L C +R V C + G + D+ K + M C+ + + +N C C+ +KC + ++ NG++ CECP C K VCG+D VTY C L AC K + + + G C E
Sbjct: 461 PCLKKICDFGGTCVVKNGE---AVCECPDA---CPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPC--------------DKACTNCS---FGAICDAQTGRCVCPTE-CVGSRQPVCGSDGTTYPNECMLNVKACTKQLELRVVAQGECNKITICSKGCWNLYVEMCVCVFDQVCVYMCICNVLIVCVHVSAECGSGGSGSGVEACEQERCRKYGGSWDEDTEDDRCVC---DFTCQN-----VCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSETVYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGGLRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGLCSCKTGVKGMKCDVCSDGSKMPANGCHKGASAPKSCTELKCL--FGASCVEENGQAHCECPSPDCDQKNKTKVCGSDGVTYADQCQLRTIACRQDKEIIVKHLGQCTE 933
BLAST of Agrin, putative vs. Ensembl Cavefish
Match: agrn (agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]) HSP 1 Score: 251.136 bits (640), Expect = 1.264e-66 Identity = 225/757 (29.72%), Postives = 332/757 (43.86%), Query Frame = 1
Query: 559 CKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQ-ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ---MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAE------NRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKAC-------------IDNKTIRVAPSVIGCISHNYIADEKNKRS------------CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
C FGA C++ P +P C C C VCGSD Y + C +++ +C +Q+ I+V C K+ C +C +TC+ + C CP C+ Q +CG+D + Y + CE+ +C + I Q+ G CD C+ ++ N C + + + P + C + P+C SD +Y SEC ++ + K+ L ++ G CQ Q C +C + A C+ N A+CSCD + C T++ P+CG DG Y N C C Q + V C+ N PC C D C + NG++ CECP + CP+ PVCGND TY S C++ C +K + I + G C+ + +C IC D +C CPT G + VCG+DG TY +EC + + AC T + L++ G C+K +IC N ++E C+C D V V +C N + V AE + A + C+ DE+ + +CVC C VCG+DG Y++ECELK+ C ++K + + + C Y E K + C CN GS CDP++ QC CK G+ G CDRC G+WNF TEN GCT C+C+ GS C TG C+CK G C VC G + +GC + +
Sbjct: 60 CGFGAVCERDPADPSKGDCLCKKVPCPL--VVAPVCGSDSSTYTNECELEKAQCSMQRRIKVMRKGPCALKDP------------CNDVTCSYGSTCIQSSDGLSAKCMCPL-SCERVPKQVVCGSDGLDYPSECELNMKACSTQKNIRLQHSGSCDPCRDIQSGLNFTC--LVNAQTRKMETSAPPDSCPPGAE-PLCASDGRSYSSECQMQRTAVLKERHLKKVHSGPCQP--QGKECKE-ECRFSAVCL-LENGRARCSCDPIHCDGTYK-PLCGKDGHTYPNDCERRNAECRTQTHIPVKQQGTCDLNAPSPCLKKIC-DFGGTCVV-KNGEAVCECP-DACPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPCD------KACTNCSFGAIC--DAQTGRCVCPT-ECVGSRQPVCGSDGTTYPNECMLNVKAC-TKQLELRVVAQGECNK--------ITICSKGCWNLYVE-MCVCVFDQVCVYMCIC--------NVLIVCVHVSAECGSGGSGSGVEACEQERCRKYGGSWDEDT-----------EDDRCVCDFTCQN----VCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSETVYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGGLRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGLCSCKTGVKGMKCDVCSDGSKMPANGCHKGA 748 HSP 2 Score: 147.132 bits (370), Expect = 8.160e-35 Identity = 166/745 (22.28%), Postives = 300/745 (40.27%), Query Frame = 1
Query: 2590 TGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP----NCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYC-QTHKPC-----LNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESL---QLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
G C+ +P G C +S + + ++ E ++ ++ KSY + Y ++ I++ +SD GI+ Y+S + D+L++ L VE + D GSG + SRVQ+ G+ H ++V R R++ L +D G +P L+ IG ++ + V+ G VGC+ L + +Y + + ++ + V C KN + + N C +H PC C E Y C+C G +G C +V ++I F S++ L L+Q++ + + L +NG++ + D FIS+ L LE G + S E + WN V + +A + +N+N G V + +K + ++ GG + + + LK F GAIQKI + I + + ++++ + PC G CVP ++ C C +++ ++ A + + G++ +Y K +K+ N + KT + G+++ K +AI +G +++ +LG K VLR T ++ N+W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GC+ ++ VD + +LV DA N IL C
Sbjct: 1181 AGPCDSQPCGHGGTCEEIRDDFSCTCPAGRGGAVCEKMINY----YIPSFGGKSYLAFPTMRAYHTVRIAMEFRASDMSGILLYNSQNGKK-DFLSLALVGGKVELKFDTGSGICSVVSRVQIKPGQWHHLVVNRNRRNAALSVDNEPPVEGQSPTGTDGLNLETHLYIGGVPEDMVQDVKERTSVSAGLVGCIRQLDVN----NMVYNLQENGGNVLFGTAVGECGNNPCSPNPCKNGASCHTKGAEMFHCKCVNAFSGPTCADSHNPCEPNPCHPSSQCAILPEGGYKCECPMGREGKHCRKVSEKKGAYIPYFDGESYLELKGLHLYNHDLKQKVSMTVVLLANDSNGMIFYNGQKTDGKGDFISLALIGGILEFRYDLGKGPAVIRSKEKIKLNVWNTVNLERAQRKGEINVN-------------GKDPVRGESPNQHNDLNLK-ESLYVGGAPDFSKVARAAS--LKSGFKGAIQKITVMGTQILKPENALHSSDVSMYSHHPCS------GDVCHNGGECVPMLDSYECVCPYGFTGAQCQHAT-YEKAAGESEAIAFDGRTFIEYHNGVTKSEKALLVNKFELSIKTEATHGLILWSGKGVERSDYI-ALAIVDGRVQMTYDLGSKPVVLRST--VRVNTNRWIRIKASRALRDGSLQVGNE---AAVTGSSPLAATQLDTDGALWLGGLEELSVARRLPKAYSTGFMGCVKDVIVDGVDLHLVEDAL-NSPKILHC 1886 HSP 3 Score: 103.99 bits (258), Expect = 1.241e-21 Identity = 168/634 (26.50%), Postives = 227/634 (35.80%), Query Frame = 1
Query: 550 KLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVT-NPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKC-QTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEP--------RCKEQGKDCPSYQICKSDLVQA----------KCEC-------PTC-PQSGLGESVCGTDGKTYKSECHMRMHACET---NSIALKIANIGTCDKCK-NKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKV---KSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVC----TDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
K C+F A C G A C C C K + CG DG Y + C + EC Q I V QQ + C + C TCV N C+CP D C S +CG D TY++ C M+ + C + I Q+ G CD C F IC D C CP C + Q PVCGSD TY +EC L +++C K++ L V+ +G+C + I S C N+ C+ V C C+ L I+ C + C + + CE C + + E + C Q VCG+D TY+S C L +T C + L + G C +P C E Y C+ A KC C TC P SG G G +R CE N + N C C + S C+ L C C T ++ VC + N C KSC E + + S C E+ NGQ C D K K +VCG+DG Y ++C+L+ AC +K I V
Sbjct: 280 KEECRFSAVCLLENGR---ARCSCDPIHCDGTYKPL--CGKDGHTYPNDCERRNAECRTQTHIPVK----------QQGTCDLNAPSPCLKKICDFGGTCVVKNGEAVCECP-DACPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPCDK-ACTNCSFGAIC----DAQTGRCVCPTE---CVGSRQ-PVCGSDGTTYPNECMLNVKACTKQLELRVVAQGECNKITICSKGCWNLYVEM-CVCVFDQVCVYMCICNVL---------------IV----CVHVSAECGSGGSGSGVEA--CEQERCR-----------KYGGSWDEDTEDDRCVCDFTCQNVCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSET-----VYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGG--------LRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGL---CSCKTGVKGMKCDVCSDGSKMPANGCHKGASAPKSCTELKCLFGAS---------------CVEE--------NGQAHCECPSPDCDQKNKTKVCGSDGVTYADQCQLRTIACRQDKEIIV 816 HSP 4 Score: 80.4925 bits (197), Expect = 1.771e-14 Identity = 124/507 (24.46%), Postives = 173/507 (34.12%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKAN---RFIEQQYVGRCDIC---------KFVKCEFNGICEMM-----------------------------------------EDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQC------------------------------HYGAKCIKTSNTVAQCSC----DYLDCKN------TWRDPV-----------CGNDGIIYDN----------KCFLMKMSCEKQMEMHVINSNFCENNP-----CSGVKCPDPYQYCRIDNNGKSTCECPKEPC-PKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNE 1701
PC K C FG C G AVC C + Q + VCG+DG Y S C M+ C LQK I + C + + S C Q+ C CP + C S +CG+D TY N C + +C R + Q + IC + C F+ +C M ED C C D C VCGSD TY SEC LK C+K++ L + +G C++K S + + + C HYG+ T QCSC L C +R V C + G + D+ K + M C+ + + +N C C+ +KC + ++ NG++ CECP C K VCG+D VTY C L AC K + + + G C E
Sbjct: 352 PCLKKICDFGGTCVVKNGE---AVCECPDA---CPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPCDKA-----CTNCSFGAICDAQT------------GRCVCPTE-CVGSRQPVCGSDGTTYPNECMLNVKACTKQLELRVVAQGECNKITICSKGCWNLYVEMCVCVFDQVCVYMCICNVLIVCVHVSAECGSGGSGSGVEACEQERCRKYGGSWDEDTEDDRCVC---DFTCQN-----VCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSETVYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGGLRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGLCSCKTGVKGMKCDVCSDGSKMPANGCHKGASAPKSCTELKCL--FGASCVEENGQAHCECPSPDCDQKNKTKVCGSDGVTYADQCQLRTIACRQDKEIIVKHLGQCTE 824
BLAST of Agrin, putative vs. Ensembl Cavefish
Match: agrn (agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]) HSP 1 Score: 250.366 bits (638), Expect = 1.851e-66 Identity = 225/757 (29.72%), Postives = 332/757 (43.86%), Query Frame = 1
Query: 559 CKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQ-ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQ---MNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAE------NRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKAC-------------IDNKTIRVAPSVIGCISHNYIADEKNKRS------------CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENS 2688
C FGA C++ P +P C C C VCGSD Y + C +++ +C +Q+ I+V C K+ C +C +TC+ + C CP C+ Q +CG+D + Y + CE+ +C + I Q+ G CD C+ ++ N C + + + P + C + P+C SD +Y SEC ++ + K+ L ++ G CQ Q C +C + A C+ N A+CSCD + C T++ P+CG DG Y N C C Q + V C+ N PC C D C + NG++ CECP + CP+ PVCGND TY S C++ C +K + I + G C+ + +C IC D +C CPT G + VCG+DG TY +EC + + AC T + L++ G C+K +IC N ++E C+C D V V +C N + V AE + A + C+ DE+ + +CVC C VCG+DG Y++ECELK+ C ++K + + + C Y E K + C CN GS CDP++ QC CK G+ G CDRC G+WNF TEN GCT C+C+ GS C TG C+CK G C VC G + +GC + +
Sbjct: 35 CGFGAVCERDPADPSKGDCLCKKVPCPL--VVAPVCGSDSSTYTNECELEKAQCSMQRRIKVMRKGPCALKDP------------CNDVTCSYGSTCIQSSDGLSAKCMCPL-SCERVPKQVVCGSDGLDYPSECELNMKACSTQKNIRLQHSGSCDPCRDIQSGLNFTC--LVNAQTRKMETSAPPDSCPPGAE-PLCASDGRSYSSECQMQRTAVLKERHLKKVHSGPCQP--QGKECKE-ECRFSAVCL-LENGRARCSCDPIHCDGTYK-PLCGKDGHTYPNDCERRNAECRTQTHIPVKQQGTCDLNAPSPCLKKIC-DFGGTCVV-KNGEAVCECP-DACPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPCD------KACTNCSFGAIC--DAQTGRCVCPT-ECVGSRQPVCGSDGTTYPNECMLNVKAC-TKQLELRVVAQGECNK--------ITICSKGCWNLYVE-MCVCVFDQVCVYMCIC--------NVLIVCVHVSAECGSGGSGSGVEACEQERCRKYGGSWDEDT-----------EDDRCVCDFTCQN----VCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSETVYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGGLRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGLCSCKTGVKGMKCDVCSDGSKMPANGCHKGA 723 HSP 2 Score: 147.132 bits (370), Expect = 7.382e-35 Identity = 166/745 (22.28%), Postives = 300/745 (40.27%), Query Frame = 1
Query: 2590 TGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP----NCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYC-QTHKPC-----LNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESL---QLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
G C+ +P G C +S + + ++ E ++ ++ KSY + Y ++ I++ +SD GI+ Y+S + D+L++ L VE + D GSG + SRVQ+ G+ H ++V R R++ L +D G +P L+ IG ++ + V+ G VGC+ L + +Y + + ++ + V C KN + + N C +H PC C E Y C+C G +G C +V ++I F S++ L L+Q++ + + L +NG++ + D FIS+ L LE G + S E + WN V + +A + +N+N G V + +K + ++ GG + + + LK F GAIQKI + I + + ++++ + PC G CVP ++ C C +++ ++ A + + G++ +Y K +K+ N + KT + G+++ K +AI +G +++ +LG K VLR T ++ N+W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GC+ ++ VD + +LV DA N IL C
Sbjct: 1156 AGPCDSQPCGHGGTCEEIRDDFSCTCPAGRGGAVCEKMINY----YIPSFGGKSYLAFPTMRAYHTVRIAMEFRASDMSGILLYNSQNGKK-DFLSLALVGGKVELKFDTGSGICSVVSRVQIKPGQWHHLVVNRNRRNAALSVDNEPPVEGQSPTGTDGLNLETHLYIGGVPEDMVQDVKERTSVSAGLVGCIRQLDVN----NMVYNLQENGGNVLFGTAVGECGNNPCSPNPCKNGASCHTKGAEMFHCKCVNAFSGPTCADSHNPCEPNPCHPSSQCAILPEGGYKCECPMGREGKHCRKVSEKKGAYIPYFDGESYLELKGLHLYNHDLKQKVSMTVVLLANDSNGMIFYNGQKTDGKGDFISLALIGGILEFRYDLGKGPAVIRSKEKIKLNVWNTVNLERAQRKGEINVN-------------GKDPVRGESPNQHNDLNLK-ESLYVGGAPDFSKVARAAS--LKSGFKGAIQKITVMGTQILKPENALHSSDVSMYSHHPCS------GDVCHNGGECVPMLDSYECVCPYGFTGAQCQHAT-YEKAAGESEAIAFDGRTFIEYHNGVTKSEKALLVNKFELSIKTEATHGLILWSGKGVERSDYI-ALAIVDGRVQMTYDLGSKPVVLRST--VRVNTNRWIRIKASRALRDGSLQVGNE---AAVTGSSPLAATQLDTDGALWLGGLEELSVARRLPKAYSTGFMGCVKDVIVDGVDLHLVEDAL-NSPKILHC 1861 HSP 3 Score: 103.605 bits (257), Expect = 1.578e-21 Identity = 168/634 (26.50%), Postives = 227/634 (35.80%), Query Frame = 1
Query: 550 KLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVT-NPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKC-QTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEP--------RCKEQGKDCPSYQICKSDLVQA----------KCEC-------PTC-PQSGLGESVCGTDGKTYKSECHMRMHACET---NSIALKIANIGTCDKCK-NKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKV---KSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVC----TDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
K C+F A C G A C C C K + CG DG Y + C + EC Q I V QQ + C + C TCV N C+CP D C S +CG D TY++ C M+ + C + I Q+ G CD C F IC D C CP C + Q PVCGSD TY +EC L +++C K++ L V+ +G+C + I S C N+ C+ V C C+ L I+ C + C + + CE C + + E + C Q VCG+D TY+S C L +T C + L + G C +P C E Y C+ A KC C TC P SG G G +R CE N + N C C + S C+ L C C T ++ VC + N C KSC E + + S C E+ NGQ C D K K +VCG+DG Y ++C+L+ AC +K I V
Sbjct: 255 KEECRFSAVCLLENGR---ARCSCDPIHCDGTYKPL--CGKDGHTYPNDCERRNAECRTQTHIPVK----------QQGTCDLNAPSPCLKKICDFGGTCVVKNGEAVCECP-DACPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPCDK-ACTNCSFGAIC----DAQTGRCVCPTE---CVGSRQ-PVCGSDGTTYPNECMLNVKACTKQLELRVVAQGECNKITICSKGCWNLYVEM-CVCVFDQVCVYMCICNVL---------------IV----CVHVSAECGSGGSGSGVEA--CEQERCR-----------KYGGSWDEDTEDDRCVCDFTCQNVCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSET-----VYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGG--------LRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGL---CSCKTGVKGMKCDVCSDGSKMPANGCHKGASAPKSCTELKCLFGAS---------------CVEE--------NGQAHCECPSPDCDQKNKTKVCGSDGVTYADQCQLRTIACRQDKEIIV 791 HSP 4 Score: 80.1073 bits (196), Expect = 2.038e-14 Identity = 123/507 (24.26%), Postives = 173/507 (34.12%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKAN---RFIEQQYVGRCDIC---------KFVKCEFNGICEMM-----------------------------------------EDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQC------------------------------HYGAKCIKTSNTVAQCSC----DYLDCKN------TWRDPV-----------CGNDGIIYDN----------KCFLMKMSCEKQMEMHVINSNFCENNP-----CSGVKCPDPYQYCRIDNNGKSTCECPKEPC-PKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNE 1701
PC K C FG C G AVC C + Q + VCG+DG Y S C M+ C LQK I + C ++ C + S + C CP + C S +CG+D TY N C + +C R + Q + IC + C F+ +C M ED C C D C VCGSD TY SEC LK C+K++ L + +G C++K S + + + C HYG+ T QCSC L C +R V C + G + D+ K + M C+ + + +N C C+ +KC + ++ NG++ CECP C K VCG+D VTY C L AC K + + + G C E
Sbjct: 327 PCLKKICDFGGTCVVKNGE---AVCECPDA---CPQSQDPVCGNDGHTYSSQCHMRAMGCALQKHISIQHKGPC--------------DKACTNCS---FGAICDAQTGRCVCPTE-CVGSRQPVCGSDGTTYPNECMLNVKACTKQLELRVVAQGECNKITICSKGCWNLYVEMCVCVFDQVCVYMCICNVLIVCVHVSAECGSGGSGSGVEACEQERCRKYGGSWDEDTEDDRCVC---DFTCQN-----VCGSDGQTYDSECELKKTRCEKQVDLLLQSQGPCESKPVSGLITPLHCSETVYGCCEDGKTAALGVELAGCPSKCHCNHYGSYGGTCDPTSGQCSCKPGVGGLRCDRCEPGFWNFRGIVTENTSGCTPCNCDSVGSVRDDCEQMTGLCSCKTGVKGMKCDVCSDGSKMPANGCHKGASAPKSCTELKCL--FGASCVEENGQAHCECPSPDCDQKNKTKVCGSDGVTYADQCQLRTIACRQDKEIIVKHLGQCTE 799 HSP 5 Score: 73.559 bits (179), Expect = 2.065e-12 Identity = 54/149 (36.24%), Postives = 75/149 (50.34%), Query Frame = 1
Query: 1933 KNKKCSFYSICQMNRFLEPQ---CIC-PTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE-DCPLGKMC------RNGQCVCTDKCSKV-KLRVCGTDGNYYENECELKRKACIDNKTIRVAPS 2343
+ K C F ++C+ + +P C+C C V PVCGSD TY NEC L+ C+ R I + +GPC L+ + C + C G C + +C+C C +V K VCG+DG Y +ECEL KAC K IR+ S
Sbjct: 31 RGKLCGFGAVCERDP-ADPSKGDCLCKKVPCPLVVAPVCGSDSSTYTNECELEKAQCSMQRRIKVMRKGPCALK------DPCNDVTCSYGSTCIQSSDGLSAKCMCPLSCERVPKQVVCGSDGLDYPSECELNMKACSTQKNIRLQHS 172
BLAST of Agrin, putative vs. Ensembl Cavefish
Match: EGFLAM (EGF like, fibronectin type III and laminin G domains [Source:HGNC Symbol;Acc:HGNC:26810]) HSP 1 Score: 130.183 bits (326), Expect = 6.500e-30 Identity = 138/633 (21.80%), Postives = 252/633 (39.81%), Query Frame = 1
Query: 2809 SSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIF-----PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTD-PEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISEL 4683
S DG++ Y + D+ D+ ++ L + +R + G+GA + ++ + +G +++ V R L +D G + T + +G + P + L+ + GF GC+ SL + +D+ P S + + D T C NGG+C + + Y C C GY+G C+E S + + + + P + + E+TFLP A +G L + D R+ F+SV + + H+E G + S E + +W+ + + + A+ L L++ L P V + TQ R + P++ GGV + S V L F G+IQK+ +ND +R + + N+ G PC+ + G C P+ + C C L R + ++ G+S Y + + S + +++++F++++ DG+L+ + S + +A+ G + + NLG LR+ G D +WHTV+ +R+ + L + R L T G +++GG K+ F Y GC+S L
Sbjct: 295 SEDGLLLYCGENDQGEGDFASLALIRGKLHFRYNCGTGAAQIAAQSPVKLGVWYTVTVYREGLSGWLRVDNDTPVTGRSQGEFTKITFRTPLYLGGS--PAVYWLARAAGTSRGFQGCVQSLAVNG--------------------------------RMIDMRPWPLGRALSGADVGECSDGVCTDVSCENGGVCYANRADGYICLCPLGYRGPLCQETFSLFLPHFNAALMPYLSAPWPRPAQYYLSFTEFEMTFLPEAFDGTLLYSEDLDSRD--FLSVVMVEGHVEFRFDCGSGTATIKSEEPVSLFRWHELRVSRTARRGILQLDN--------LRP-----VEGMAEGAFTQIRCSS-PLYIGGVPNYNLVKNSASVLLP--FTGSIQKVTVNDRVVRLTAASVKGVNV--------GNAVHPCADSLCANAGVCRPKHDGYECDCP-----LGYRGKHCQSADSGAVEIPQFTGRSYLMYDSKDILKRISGPRTHVQLRFRSSAQDGLLMWIGDTNMRHNSDYMFLALHGGTVVFSYNLGSGTNTLRVNG--TFIDGRWHTVRAVRDGQMGKLSVGSSTTRVGK-SPGRMRQLNTSGALYIGGIKE--ASFHIPYLRGLVGCMSHL 857 HSP 2 Score: 85.1149 bits (209), Expect = 4.170e-16 Identity = 94/403 (23.33%), Postives = 169/403 (41.94%), Query Frame = 1
Query: 2629 YCSVCPIGYSISNHGCKEN-SIYENKVDAVELGFLKDSFAKSYWSGNAENYLSIN---ISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS---HIWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKK 3804
Y +CP+GY C+E S++ +A + +L + W A+ YLS ++ +P + DG + Y D D S D+L++V+ +VE+R D GSG +KS +++ R H + V R R L +D GM T + IG P N + ++ V F G + K+T + + +++VK ++ N+V D C N G+C+ + Y C C GY+G C+ S I +F S++++ S+ + R R +++ F A +GLL +I D + R N ++ + L+ + + G L + + G+W+ V ++ + L++ S + K
Sbjct: 464 YICLCPLGYR--GPLCQETFSLFLPHFNAALMPYLS-----APWPRPAQYYLSFTEFEMTFLPEAFDGTLLYSEDLD-SRDFLSVVMVEGHVEFRFDCGSGTATIKSEEPVSLFRWHELRVSRTARRGILQLDNLRPVEGMAEGAFTQIRCSSPLYIGGV--PNYNLVKNSASVLLPFTGSIQ--KVTVNDRVV----------RLTAASVK----GVNVGNAVHPCAD---------------------SLCANAGVCR-PKHDGYECDCPLGYRGKHCQSADSGAVEIPQFTGRSYLMYDSKDILKRISGPRTHVQLRFRSSAQDGLLMWIGDTNMRHNSDYMFLALHGGTVVFSYNLGSGTNTLRVNGTFIDGRWHTVRAVRDGQMGKLSVGSSTTRVGK 818
BLAST of Agrin, putative vs. Ensembl Cavefish
Match: EGFLAM (EGF like, fibronectin type III and laminin G domains [Source:HGNC Symbol;Acc:HGNC:26810]) HSP 1 Score: 130.183 bits (326), Expect = 7.824e-30 Identity = 138/633 (21.80%), Postives = 252/633 (39.81%), Query Frame = 1
Query: 2809 SSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIF-----PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTD-PEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISEL 4683
S DG++ Y + D+ D+ ++ L + +R + G+GA + ++ + +G +++ V R L +D G + T + +G + P + L+ + GF GC+ SL + +D+ P S + + D T C NGG+C + + Y C C GY+G C+E S + + + + P + + E+TFLP A +G L + D R+ F+SV + + H+E G + S E + +W+ + + + A+ L L++ L P V + TQ R + P++ GGV + S V L F G+IQK+ +ND +R + + N+ G PC+ + G C P+ + C C L R + ++ G+S Y + + S + +++++F++++ DG+L+ + S + +A+ G + + NLG LR+ G D +WHTV+ +R+ + L + R L T G +++GG K+ P Y GC+S L
Sbjct: 383 SEDGLLLYCGENDQGEGDFASLALIRGKLHFRYNCGTGAAQIAAQSPVKLGVWYTVTVYREGLSGWLRVDNDTPVTGRSQGEFTKITFRTPLYLGGS--PAVYWLARAAGTSRGFQGCVQSLAVNG--------------------------------RMIDMRPWPLGRALSGADVGECSDGVCTDVSCENGGVCYANRADGYICLCPLGYRGPLCQETFSLFLPHFNAALMPYLSAPWPRPAQYYLSFTEFEMTFLPEAFDGTLLYSEDLDSRD--FLSVVMVEGHVEFRFDCGSGTATIKSEEPVSLFRWHELRVSRTARRGILQLDN--------LRP-----VEGMAEGAFTQIRCSS-PLYIGGVPNYNLVKNSASVLLP--FTGSIQKVTVNDRVVRLTAASVKGVNV--------GNAVHPCADSLCANAGVCRPKHDGYECDCP-----LGYRGKHCQSADSGAVEIPQFTGRSYLMYDSKDILKRISGPRTHVQLRFRSSAQDGLLMWIGDTNMRHNSDYMFLALHGGTVVFSYNLGSGTNTLRVNG--TFIDGRWHTVRAVRDGQMGKLSVGSSTTRVGK-SPGRMRQLNTSGALYIGGIKEASFHIP--YLRGLVGCMSHL 945 HSP 2 Score: 85.1149 bits (209), Expect = 5.039e-16 Identity = 94/403 (23.33%), Postives = 169/403 (41.94%), Query Frame = 1
Query: 2629 YCSVCPIGYSISNHGCKEN-SIYENKVDAVELGFLKDSFAKSYWSGNAENYLSIN---ISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS---HIWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKK 3804
Y +CP+GY C+E S++ +A + +L + W A+ YLS ++ +P + DG + Y D D S D+L++V+ +VE+R D GSG +KS +++ R H + V R R L +D GM T + IG P N + ++ V F G + K+T + + +++VK ++ N+V D C N G+C+ + Y C C GY+G C+ S I +F S++++ S+ + R R +++ F A +GLL +I D + R N ++ + L+ + + G L + + G+W+ V ++ + L++ S + K
Sbjct: 552 YICLCPLGYR--GPLCQETFSLFLPHFNAALMPYLS-----APWPRPAQYYLSFTEFEMTFLPEAFDGTLLYSEDLD-SRDFLSVVMVEGHVEFRFDCGSGTATIKSEEPVSLFRWHELRVSRTARRGILQLDNLRPVEGMAEGAFTQIRCSSPLYIGGV--PNYNLVKNSASVLLPFTGSIQ--KVTVNDRVV----------RLTAASVK----GVNVGNAVHPCAD---------------------SLCANAGVCR-PKHDGYECDCPLGYRGKHCQSADSGAVEIPQFTGRSYLMYDSKDILKRISGPRTHVQLRFRSSAQDGLLMWIGDTNMRHNSDYMFLALHGGTVVFSYNLGSGTNTLRVNGTFIDGRWHTVRAVRDGQMGKLSVGSSTTRVGK 906
BLAST of Agrin, putative vs. Ensembl Sea Lamprey
Match: FST (follistatin [Source:HGNC Symbol;Acc:HGNC:3971]) HSP 1 Score: 93.9745 bits (232), Expect = 1.423e-21 Identity = 74/236 (31.36%), Postives = 107/236 (45.34%), Query Frame = 1
Query: 1258 DICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCE-CPKEPCPK---------IVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECP----TCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTC 1923
+ C N+ C G +C + +C C DC N R VCG DG Y + C L+K C+ Q + + C+ N C V+CP +C +D N + C C PCP Q VCG D VTY S+C+L R CL K + + Y G C++ C + C S + C D + C C + E VC T+ TY + C M AC ++ + L++ + G C
Sbjct: 1 ETCDNVDCGPGKQCRMSRRNKPRCVC-APDCSNASRASVCGTDGKTYRDGCALLKARCKGQPNLEMQYHGPCQKN-CKDVQCPS-GTFCVVDQNNNAHCVLCNLRPCPAESGQGEQQQQQQHVCGKDGVTYASVCHLRRATCLLGKSIGVAYQGRCSKSKSCDDV--LCGSGKKCLWDPAEGGPHCAQCNDICRDAKRMEPVCATNNNTYPNACAMGNAAC-SSGVYLEVKHTGYC 230 HSP 2 Score: 83.5741 bits (205), Expect = 5.959e-18 Identity = 67/215 (31.16%), Postives = 99/215 (46.05%), Query Frame = 1
Query: 865 PYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDI-CKFVKCEFNGICEMMEDGLGTICKC---PNPDE---LCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCI----KTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCE 1476
P C C D ++S + +CGTD TY + C + K CK +E QY G C CK V+C C + ++ C P P E + Q VCG D VTY S CHL+ +C S+ V Y+G+C +S C ++ C G KC+ + AQC+ D K +PVC + Y N C + +C + + V ++ +C
Sbjct: 22 PRCVCAPDCSNASRASVCGTDGKTYRDGCALLKARCKGQPNLEMQYHGPCQKNCKDVQCPSGTFCVVDQNNNAHCVLCNLRPCPAESGQGEQQQQQQHVCGKDGVTYASVCHLRRATCLLGKSIGVAYQGRCS---KSKSCDDVLCGSGKKCLWDPAEGGPHCAQCNDICRDAKRM--EPVCATNNNTYPNACAMGNAACSSGVYLEVKHTGYCS 231 HSP 3 Score: 62.003 bits (149), Expect = 1.277e-10 Identity = 65/250 (26.00%), Postives = 100/250 (40.00%), Query Frame = 1
Query: 544 CSKLNCKFGAQCQKWPGNPPTAVC--HCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVT--NPYPYC------KCPNDK----CDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCD---ICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQV-PVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKC 1239
C ++C G QC+ N P VC CSN SVCG+DG Y C + + C Q ++ E Q G + N C+ CP CV N +C CP + +CG D +TY ++C +++ +C + I Y GRC C V C C + D C +++C ++ PVC +++ TY + C + +C + L V + G C
Sbjct: 3 CDNVDCGPGKQCRMSRRNKPRCVCAPDCSNAS------RASVCGTDGKTYRDGCALLKARCKGQPNL-----------EMQYHGPCQKN---CKDVQCPSGTFCVVDQNNNAHCVLCNLRPCPAESGQGEQQQQQQHVCGKDGVTYASVCHLRRATCLLGKSIGVAYQGRCSKSKSCDDVLCGSGKKC--LWDPAEGGPHCAQCNDICRDAKRMEPVCATNNNTYPNACAMGNAACSSGVYLEVKHTGYC 230 HSP 4 Score: 61.2326 bits (147), Expect = 2.572e-10 Identity = 57/211 (27.01%), Postives = 87/211 (41.23%), Query Frame = 1
Query: 1102 PDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCS-CDYLDCK--------NTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFC-ENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPC--PKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICN 1698
PD C+ + VCG+D TY+ C L CK + +L + Y G CQ C ++QC G C+ N A C C+ C + VCG DG+ Y + C L + +C + V C ++ C V C + G C + C K ++PVC + TY + C + AC + L + + G C+
Sbjct: 28 PD--CSNASRASVCGTDGKTYRDGCALLKARCKGQPNLEMQYHGPCQKN-----CKDVQCPSGTFCVVDQNNNAHCVLCNLRPCPAESGQGEQQQQQQHVCGKDGVTYASVCHLRRATCLLGKSIGVAYQGRCSKSKSCDDVLCGSGKKCLWDPAEGGPHCAQCNDICRDAKRMEPVCATNNNTYPNACAMGNAACSSGVYLEVKHTGYCS 231 HSP 5 Score: 56.225 bits (134), Expect = 1.145e-8 Identity = 66/234 (28.21%), Postives = 89/234 (38.03%), Query Frame = 1
Query: 1486 CSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAK----CECPTCPQSGLGES-------VCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDK---CKNKKCSFYSICQMNRFLE-PQCI-CPTDCVYVRR--PVCGSDDRTYENECFLKVKSCAENRLITAVSEGPC 2133
C V C P + CR+ K C C + VCG D TY C L + C + L + Y+G C + CK+ CPS C D C CP VCG DG TY S CH+R C ++ +A G C K C + C C + P C C C +R PVC +++ TY N C + +C+ + G C
Sbjct: 3 CDNVDC-GPGKQCRMSRRNKPRCVCAPDCSNASRASVCGTDGKTYRDGCALLKARCKGQPNLEMQYHGPCQKN--CKDV--QCPSGTFCVVDQNNNAHCVLCNLRPCPAESGQGEQQQQQQHVCGKDGVTYASVCHLRRATCLLGK-SIGVAYQGRCSKSKSCDDVLCGSGKKCLWDPAEGGPHCAQCNDICRDAKRMEPVCATNNNTYPNACAMGNAACSSGVYLEVKHTGYC 230 HSP 6 Score: 56.225 bits (134), Expect = 1.200e-8 Identity = 46/156 (29.49%), Postives = 67/156 (42.95%), Query Frame = 1
Query: 1924 DKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRP-VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE-DCPLGKMC-----RNGQCVCTD-----------KCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVA 2337
+ C N C C+M+R +P+C+C DC R VCG+D +TY + C L C + GPC ++ C + CP G C N CV + + + + VCG DG Y + C L+R C+ K+I VA
Sbjct: 1 ETCDNVDCGPGKQCRMSRRNKPRCVCAPDCSNASRASVCGTDGKTYRDGCALLKARCKGQPNLEMQYHGPC--------QKNCKDVQCPSGTFCVVDQNNNAHCVLCNLRPCPAESGQGEQQQQQQHVCGKDGVTYASVCHLRRATCLLGKSIGVA 148 HSP 7 Score: 48.521 bits (114), Expect = 3.677e-6 Identity = 53/192 (27.60%), Postives = 80/192 (41.67%), Query Frame = 1
Query: 1816 SVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDK-CKNKKCSFYSICQMNRFLEPQCI------CPTDCVYVRRP-----VCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQ-----FIKPDEERCPEDCPL--GKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
SVCGTDGKTY+ C + C+ L++ G C K CK+ +C + C +++ C+ CP + + VCG D TY + C L+ +C + I +G C + ++C D P G C +C D +K VC T+ N Y N C + AC + V
Sbjct: 37 SVCGTDGKTYRDGCALLKARCKGQP-NLEMQYHGPCQKNCKDVQCPSGTFCVVDQNNNAHCVLCNLRPCPAESGQGEQQQQQQHVCGKDGVTYASVCHLRRATCLLGKSIGVAYQGRCSKSKSCDDVLCGSGKKCLWD-PAEGGPHCAQCNDICRD--AKRMEPVCATNNNTYPNACAMGNAACSSGVYLEV 224
BLAST of Agrin, putative vs. Ensembl Sea Lamprey
Match: tmeff2a (transmembrane protein with EGF-like and two follistatin-like domains 2a [Source:ZFIN;Acc:ZDB-GENE-070912-622]) HSP 1 Score: 67.3958 bits (163), Expect = 4.798e-12 Identity = 40/125 (32.00%), Postives = 65/125 (52.00%), Query Frame = 1
Query: 1132 VPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQ-----------------CHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHV 1455
VPVCGSD ++YQ+ECHL+ +C+ + +T G C T++ S + C YG++C + + + C C+ +DC +PVC +DG+ Y+N C + + SC KQ + V
Sbjct: 7 VPVCGSDGLSYQNECHLRQTACRLQRIITQAAPGPCYTELGSGSSNGADYEGSGSEHYRKSTKCGICLYGSECDEDAEDIG-CVCN-IDCTGHNSNPVCASDGVSYENPCLVKEASCMKQQLIEV 129 HSP 2 Score: 54.6842 bits (130), Expect = 6.069e-8 Identity = 56/202 (27.72%), Postives = 80/202 (39.60%), Query Frame = 1
Query: 2029 PVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIK-----PDEERCPED----------CPLGKMCRNGQ----CVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIRVA------PSVIGCISHNYIA-------DEKNKRSCLCNSIGSL----------NHECDPSTTQ----CICKTGITGQYCD 2493
PVCGSD +Y+NEC L+ +C R+IT + GPC + D E + C G C CVC C+ VC +DG YEN C +K +C+ + I V P++ C ++I +++ + G L N +C S TQ CIC G GQ C+
Sbjct: 8 PVCGSDGLSYQNECHLRQTACRLQRIITQAAPGPCYTELGSGSSNGADYEGSGSEHYRKSTKCGICLYGSECDEDAEDIGCVCNIDCTGHNSNPVCASDGVSYENPCLVKEASCMKQQLIEVRFLGKCNPALCVCTVWDHIYLMSTDYNSTRDQEEPVVRDRGELCPESLQNYCRNGQCHYSHTQRHASCICDPGFLGQQCE 209 HSP 3 Score: 51.2174 bits (121), Expect = 9.703e-7 Identity = 43/137 (31.39%), Postives = 61/137 (44.53%), Query Frame = 1
Query: 913 ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRC--------------------DICKFVKCEFNGIC----EMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKI 1251
+CG+D ++Y N C +++ +C+ R I Q G C K KC GIC E ED C C N D CT + PVC SD V+Y++ C +K SC K+ + V + GKC +
Sbjct: 9 VCGSDGLSYQNECHLRQTACRLQRIITQAAPGPCYTELGSGSSNGADYEGSGSEHYRKSTKC---GICLYGSECDEDAEDIGCVC-NID--CTGHNSNPVCASDGVSYENPCLVKEASCMKQQLIEVRFLGKCNPAL 139
BLAST of Agrin, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007784.1 (pep scaffold:Pmarinus_7.0:GL477336:382472:417638:1 gene:ENSPMAG00000007035.1 transcript:ENSPMAT00000007784.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 66.2402 bits (160), Expect = 5.766e-11 Identity = 50/163 (30.67%), Postives = 77/163 (47.24%), Query Frame = 1
Query: 2374 ADEKNKRSCLCNSIGSLNHECDPSTTQCICKTG-ITGQYCDRCIDGYWNFTE--NGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYS---ISN-HGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSD 2841
D R C C+ GS + CD ++ QC C + + G+ C RC Y+ F + C C+C+ GS C+ +TG+C+CK LAMG+ C C G S SN GC + + A LG + ++ +A N S ++ + Y +D I+ D
Sbjct: 681 GDASGCRPCDCHERGSAHAACDGNSGQCACTSHTVGGRRCHRCAHSYYGFAQELGRCRECACSTAGSLDTSCHPDTGQCSCKALAMGRRCDACTHGASHLRASNPFGCSKTPSQQPPPVARALG--SSAIGLAWSPPDAPN--SDLLTYILYRNDSQIYSARD 839
BLAST of Agrin, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000128.1 (pep scaffold:Pmarinus_7.0:GL482156:314:14887:1 gene:ENSPMAG00000000114.1 transcript:ENSPMAT00000000128.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 60.077 bits (144), Expect = 3.957e-9 Identity = 27/78 (34.62%), Postives = 43/78 (55.13%), Query Frame = 1
Query: 2410 SIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVC 2643
++ +L +CDP + +C C +TG+ C+ C GYWN+ NGC C C + C+ +G+C+CK G+ C C
Sbjct: 547 AVAALGSQCDPRSGRCRCPPSVTGRRCELCQPGYWNYGPNGCQRCECT-----TGSCDPHSGRCSCKGGVSGEQCDQC 619 HSP 2 Score: 53.5286 bits (127), Expect = 3.870e-7 Identity = 32/90 (35.56%), Postives = 44/90 (48.89%), Query Frame = 1
Query: 2395 SCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTE-NGCTACSCN--RFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGY 2655
+C C+ G CD + +CICK G+ G C C GY+ F GC C C GS+ C+ +G+C C P G+ C +C GY
Sbjct: 496 ACRCDKCGMKG--CDGRSGKCICKLGVMGHSCSTCEPGYYGFDSCKGCEPCECAVAALGSQ---CDPRSGRCRCPPSVTGRRCELCQPGY 580
BLAST of Agrin, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009682.1 (pep scaffold:Pmarinus_7.0:GL479835:738:11671:-1 gene:ENSPMAG00000008750.1 transcript:ENSPMAT00000009682.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.151 bits (139), Expect = 6.619e-9 Identity = 31/79 (39.24%), Postives = 45/79 (56.96%), Query Frame = 1
Query: 2398 CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYC 2634
C CN +GS + +CD +C CK G+ G+ CD+C+ G+ + E GC C+CN G+ C +TG+C CK G C
Sbjct: 259 CNCNPVGSYSRQCD-EQGRCSCKQGVMGERCDQCLPGFHSMDEAGCRPCTCNPAGTLED-CQPDTGRCVCKDNVEGYNC 335
BLAST of Agrin, putative vs. Ensembl Nematostella
Match: EDO40386 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7E5]) HSP 1 Score: 207.994 bits (528), Expect = 2.950e-56 Identity = 189/606 (31.19%), Postives = 268/606 (44.22%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATC-VTNPYPYCKCPNDK-CDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQ-CHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPK-EPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTD-CVYVRRPVCGSDDRTYENECFLKVKSCAEN---RLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIR 2331
DPC + C AQC T VC S C VCG+DG Y++ CL++ C +IQV+ CG A+ G S C C + C V P C C + + C SS +C T T+ C M+ +C +R + G CD C VKC+ + C + D + C CP+ E C + VCG+D +Y +EC +K +C+K+ S+TV ++G C CS + C + AKCI ++ C+C + D VCG+DG Y +C + +C + ++ V + C S + Y C +G C CPK E CP + PVCG D TY S C + +AC KK + + + G C ++ CP ++ C D VCG+D +TY + C +++ C+T A+ + G CD C KC + S C C CPTD C PVCGSD +TYENEC L+V+SC N R+I+ C L C N C+C C VCG DG Y+N C L R+AC + IR
Sbjct: 11 DPCFAVVCANHAQCVTLSDGRTTCVCP-SAIDCPGVPSP--VCGTDGKTYNNDCLLRATACHNGSNIQVAGLGQCG--TAKFLGFSSLGP--CASLRCKSPSRCQVIKGKPQCVCRDVRECPSSMDPVCSTTGETFITKCHMEVEACTESRSMMVARRGECDPCSRVKCKEDRHCVVDMD-MKPKCICPSESE-CPLTVDT-VCGTDKSSYLNECVMKARACRKEKSVTVAHRGFC------GACSLAKPCEHRAKCISNTDGTLTCTCRKEENCPGRADYVCGSDGNSYFTECHMDATACRESRDITVKHKGPCGKTTHSTQQIRTFYGVCVGVEDGSMKCVCPKPEECPYVNAPVCGTDDRTYPSECIMKTSACADKKAVRVKHAGECGLT---RQAVCACPRFEDCPRDF----------------RPVCGSDLRTYVNLCRLQVEVCQTGR-AVTVLRQGACDPCSVSKCKYNSECVKRADGSTTCQCPTDRCPKEASPVCGSDGKTYENECKLRVESCKANQNVRIISRTKCNACTLSTCNLVYGTC------SASSANASCICPTNCPSDWDPVCGDDGVTYQNLCHLLREACTSGRIIR 574 HSP 2 Score: 201.06 bits (510), Expect = 6.929e-54 Identity = 170/542 (31.37%), Postives = 246/542 (45.39%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCP---NDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCE---FNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCD-YLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTC---------DKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLIT 2112
DPCS++ CK C P +C S ++C +VCG+D Y + C+MK + C +KS+ V+ CG + S + C+H+ A C++N C + C + +CG+D +Y C M +C+ +R I ++ G C + F G+C +EDG C CP P+E + PVCG+DD TY SEC +K +C K ++ V + G+C Q A C+C + DC +R PVCG+D Y N C L C+ + V+ C+ PCS KC + C +G +TC+CP + CPK PVCG+D TYE+ C L +C + + II CN C + Y C + A C CPT CP + VCG DG TY++ CH+ AC + I ++ G C D C KKC +Y C++ +C CP C PVC SD RTY+NEC K +C + R +T
Sbjct: 165 DPCSRVKCKEDRHCVVDMDMKPKCICP-SESECPLTVD--TVCGTDKSSYLNECVMKARACRKEKSVTVAHRGFCG---------ACSLAKPCEHR-----AKCISNTDGTLTCTCRKEENCPGRADYVCGSDGNSYFTECHMDATACRESRDITVKHKGPCGKTTHSTQQIRTFYGVCVGVEDG-SMKCVCPKPEEC--PYVNAPVCGTDDRTYPSECIMKTSACADKKAVRVKHAGECGLTRQ----------------------AVCACPRFEDCPRDFR-PVCGSDLRTYVNLCRLQVEVCQTGRAVTVLRQGACD--PCSVSKCKYNSE-CVKRADGSTTCQCPTDRCPKEASPVCGSDGKTYENECKLRVESCKANQNVRIISRTKCNA---CTLSTCN-LVYGTCSASSANASCICPTNCPSDW--DPVCGDDGVTYQNLCHLLREACTSGRIIRRLYR-GVCGKAVVSPRPDACAAKKCRYYGQCRVGSDGIAECACPLSCPSTADPVCASDGRTYQNECLAKKYACEKKRDLT 653 HSP 3 Score: 167.162 bits (422), Expect = 9.321e-43 Identity = 151/510 (29.61%), Postives = 223/510 (43.73%), Query Frame = 1
Query: 1015 DICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTK-----IQSDICSNIQCHYGAKCIKTSNTVAQCSC-DYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEP-CPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDL-VQAKCEC---PTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKC---SFYSICQMNRFLEPQCICPT--DCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIK----PDEERCPED---------------------------------------CPLGKMCRNGQCV-----------CTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
D C V C + C + DG T C CP+ + + PVCG+D TY ++C L+ +C ++ V G+C T C++++C ++C + QC C D +C ++ DPVC G + KC + +C + M V C+ PCS VKC + ++C +D + K C CP E CP V VCG DK +Y + C + AC +K + + + G C K C C S+ C C CP G + VCG+DG +Y +ECHM AC S + + + G C K + +FY +C +C+CP +C YV PVCG+DDRTY +EC +K +CA+ + + G C L P E CP D C + K N +CV TD+C K VCG+DG YENEC+L+ ++C N+ +R+
Sbjct: 11 DPCFAVVCANHAQCVTLSDGR-TTCVCPSAIDC--PGVPSPVCGTDGKTYNNDCLLRATACHNGSNIQVAGLGQCGTAKFLGFSSLGPCASLRCKSPSRC-QVIKGKPQCVCRDVRECPSSM-DPVCSTTGETFITKCHMEVEACTESRSMMVARRGECD--PCSRVKCKED-RHCVVDMDMKPKCICPSESECPLTVDTVCGTDKSSYLNECVMKARACRKEKSVTVAHRGFCGA----CSLAKPCEHRAKCISNTDGTLTCTCRKEENCP--GRADYVCGSDGNSYFTECHMDATACR-ESRDITVKHKGPCGKTTHSTQQIRTFYGVCVGVEDGSMKCVCPKPEECPYVNAPVCGTDDRTYPSECIMKTSACADKKAVRVKHAGECGLTRQAVCACPRFEDCPRDFRPVCGSDLRTYVNLCRLQVEVCQTGRAVTVLRQGACDPCSVSKCKYNSECVKRADGSTTCQCPTDRCPKEASPVCGSDGKTYENECKLRVESCKANQNVRI 505 HSP 4 Score: 125.176 bits (313), Expect = 3.513e-29 Identity = 117/391 (29.92%), Postives = 173/391 (44.25%), Query Frame = 1
Query: 1222 MYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSC-DYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCEN---------NPCSGVKCPDPYQYCRIDNNGKSTCECPK-EPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDL-VQAKCECPT---CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCK-NKKCSFYSICQMNRFLEPQCICPTD--CVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVC--TDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
M+ +C I D C + C A+C+ S+ C C +DC PVCG DG Y+N C L +C + V C PC+ ++C P + C++ GK C C CP + PVC T+ + C++ AC + + + G C+ R K C + C D+ ++ KC CP+ CP + ++VCGTD +Y +EC M+ AC ++ +A+ G C C K C + C N C C + C VCGSD +Y EC + +C E+R IT +GPC + R +G + +CVC ++C V VCGTD Y +EC +K AC D K +RV
Sbjct: 1 MFYAQCVLPI--DPCFAVVCANHAQCVTLSDGRTTCVCPSAIDCPGVP-SPVCGTDGKTYNNDCLLRATACHNGSNIQVAGLGQCGTAKFLGFSSLGPCASLRCKSPSR-CQV-IKGKPQCVCRDVRECPSSMDPVCSTTGETFITKCHMEVEACTESRSMMVARRGECDPCSRVK-----CKEDRHCVVDMDMKPKCICPSESECPLT--VDTVCGTDKSSYLNECVMKARACRKEK-SVTVAHRGFCGACSLAKPCEHRAKCISNTDGTLTCTCRKEENCPGRADYVCGSDGNSYFTECHMDATACRESRDITVKHKGPCGKTTHSTQQIRTFYGVCVGVEDGSMKCVCPKPEECPYVNAPVCGTDDRTYPSECIMKTSACADKKAVRV 378
BLAST of Agrin, putative vs. Ensembl Nematostella
Match: EDO40367 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7B8]) HSP 1 Score: 137.887 bits (346), Expect = 2.575e-34 Identity = 144/545 (26.42%), Postives = 211/545 (38.72%), Query Frame = 1
Query: 649 KEISVCGSDGIFYDSHCLMKRQECFLQKSIQVS------------------PNHICGRKEAQQDGISKSNEQFCQHQSC----PPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFI---EQQYVGR-------CD-----ICKFVKCEFNGICEMMEDG-----LGTICKCPNPD-------ELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECP-TCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPC 2133
K VCGSD + Y S C ++R C I P ICG + C++ +C P V + C CP +C +CG+D TY N C M++ +C N++I +Q+ G+ CD +C +F C+M +D T C D E C K ++ PV G+D+ Y +EC LK+ +CK + + G + S + C+C +C +T DPVCG D YDN+C + + +C + V C +GK+TCEC E CPK ++PVCG+D Y++ C + AC T P + CK+ +A+C C CP+S + VCGTD TY +EC ++ CET NR + P C+CP DC VCGSD+ TY NEC +K ++C N + +G C
Sbjct: 7 KSDPVCGSDNVTYASECQLRRAACLNDTWITTQRKGDCACSCPSSCGDESLPQPICGSNNKTYANECELRMDSCKNNNCVVHLGPRCANVPSGKISCTCP--ECSKREDPVCGSDSKTYPNECRMRQEACWNNKWIIVAQQEECGQIYCVCPECDNTESPVCTNDGKKFPNECQMRQDACFNKQWTTPISCAQDDQPTCVCVEPCPKTLK-PVYGTDNKNYDNECLLKLAACKSNTRILIAGFG-----------------------RYSKQIPVCACPE-NCSSTV-DPVCGTDNNTYDNECLMRQQACVANATVAVRRKGHCA-------------------KDGKATCEC-SEDCPKTLKPVCGSDNNDYDNECLMQARACST-------------------------PPHSTCKAVGDKAECVCSKVCPRS--LDLVCGTDNITYNNECFLKRQGCET-----------------------------NRTITPMCVCPKDCPASLDLVCGSDNITYSNECLMKYQACRTNSALKVKRKGDC 447 HSP 2 Score: 127.487 bits (319), Expect = 8.089e-31 Identity = 136/515 (26.41%), Postives = 203/515 (39.42%), Query Frame = 1
Query: 877 CPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNP--DELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFC-------------ENNPCS--GVKCPDPYQYCRID--------------NNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTI 2328
CP+ C S +CG+D +TY + C++++ +C + +I Q G C C CP+ DE ++ P+CGS++ TY +EC L+++SCK +N H G +C + C+C + DPVCG+D Y N+C + + +C + V C E+ C+ G K P+ Q R D + + TC C EPCPK ++PV G D Y++ C L AC + + I G R +Q C + C S + + VCGTD TY +EC MR AC N+ + + G C K + C C DC +PVCGSD+ Y+NEC ++ ++C+ T + G +CVC+ C + VCGTD Y NEC LKR+ C N+TI
Sbjct: 1 CPD--CKDKSDPVCGSDNVTYASECQLRRAACLNDTWITTQRKGDC-----------------------ACSCPSSCGDE----SLPQPICGSNNKTYANECELRMDSCKN---------------------NNCVVHLGPRCANVPSGKISCTCPEC---SKREDPVCGSDSKTYPNECRMRQEACWNNKWIIVAQQEECGQIYCVCPECDNTESPVCTNDGKKFPNECQM-RQDACFNKQWTTPISCAQDDQPTCVC-VEPCPKTLKPVYGTDNKNYDNECLLKLAACKSNTRILIAGFG------RYSKQIPVCACPENCSSTV----------------DPVCGTDNNTYDNECLMRQQACVANAT-VAVRRKGHCAK----------------DGKATCECSEDCPKTLKPVCGSDNNDYDNECLMQARACSTPPHSTCKAVG------------------------DKAECVCSKVCPRSLDLVCGTDNITYNNECFLKRQGCETNRTI 397 HSP 3 Score: 119.783 bits (299), Expect = 2.861e-28 Identity = 116/405 (28.64%), Postives = 157/405 (38.77%), Query Frame = 1
Query: 1120 KNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
K+ PVCGSD+VTY SEC L+ +C +T KG C CSC + P+CG++ Y N+C L SC K V C N P +GK +C CP+ C K PVCG+D TY + C + + AC K WII Q ++C Q C CP C VC DGK + +EC MR AC I+ C + +P C+C C +PV G+D++ Y+NEC LK+ +C N I G Q P+ C C + CS VCGTD N Y+NEC ++++AC+ N T+ V
Sbjct: 5 KDKSDPVCGSDNVTYASECQLRRAACLNDTWITTQRKGDCA----------------------------CSCPSSCGDESLPQPICGSNNKTYANECELRMDSC-KNNNCVVHLGPRCANVP-----------------SGKISCTCPE--CSKREDPVCGSDSKTYPNECRMRQEACWNNK--WIIV-----------AQQEEC----------GQIYCVCPEC--DNTESPVCTNDGKKFPNECQMRQDACFNKQWTTPIS------------------CAQDD--QPTCVCVEPCPKTLKPVYGTDNKNYDNECLLKLAACKSNTRILIAGFGRYSKQI------------PV--------CACPENCSSTVDPVCGTDNNTYDNECLMRQQACVANATVAV 296 HSP 4 Score: 102.449 bits (254), Expect = 1.587e-22 Identity = 129/484 (26.65%), Postives = 189/484 (39.05%), Query Frame = 1
Query: 538 DPCSKLNC--KFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGR-------------KEAQQDGISKSNEQFCQHQSC-----PPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDK 1929
D C NC G +C N P+ C+ +C ++E VCGSD Y + C M+++ C+ K I V+ CG+ DG NE + +C +C + P C C + C + + GTD Y N C +K +CK+N I GR + K +C CP E C+ + PVCG+D+ TY +EC ++ ++C ++ V KG C K +CS DC T + PVCG+D YDN+C + +C P+ C+ + K+ C C K CP+ + VCG D +TY + C L R C T + + + +C KDCP+ DL VCG+D TY +EC M+ AC TNS ALK+ G C +
Sbjct: 78 DSCKNNNCVVHLGPRC----ANVPSGKISCTCPEC--SKREDPVCGSDSKTYPNECRMRQEACWNNKWIIVAQQEECGQIYCVCPECDNTESPVCTNDGKKFPNECQMRQDACFNKQWTTPISCAQDDQPTCVCV-EPCPKTLKPVYGTDNKNYDNECLLKLAACKSNTRILIAGFGR-----YSK-------------QIPVCACP---ENCSSTVD-PVCGTDNNTYDNECLMRQQACVANATVAVRRKG--------------------HCAKDGKATCECS---EDCPKTLK-PVCGSDNNDYDNECLMQARACSTP-----------------------PHSTCKAVGD-KAECVCSKV-CPRSLDLVCGTDNITYNNECFLKRQGCETNRTITPMC--VC---------PKDCPA----SLDL------------------VCGSDNITYSNECLMKYQACRTNS-ALKVKRKGDCGR 449
BLAST of Agrin, putative vs. Ensembl Nematostella
Match: EDO40374 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7C5]) HSP 1 Score: 120.168 bits (300), Expect = 1.225e-29 Identity = 106/339 (31.27%), Postives = 151/339 (44.54%), Query Frame = 1
Query: 1321 AQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVA 2337
A C+C L C N DPVCG+DG YDN C L + +C+ + +I+ + C + GK TC C EPCPKI++PV G+D Y++ C L AC +K + I +G +++P C +C CP+ + + V G+DGK Y +EC +++ AC++ S L IA G DK P C+C C + +PV GSD + Y+NEC LK+ +C I G LQ KP CVC + C ++ V G+DG Y+NEC LK AC I +A
Sbjct: 7 AVCTC--LSCPNML-DPVCGSDGKNYDNVCKLRQNACKTNTLITLISRDACLQD-------------------GKPTCVC-VEPCPKILKPVYGSDGKNYDNECLLKLAACKSKSRILIAGSGRYHDKPTC-----------VCVE----------PCPE--ILKPVYGSDGKDYDNECLLKLAACKSKSRIL-IAGFGH-DK-------------------PTCVCVEPCPEILKPVYGSDGKDYDNECLLKLAACKSKSRILIAGFG---LQDDKP------------------TCVCVEPCPEILKPVYGSDGKDYDNECLLKLAACKSKSRILIA 257 HSP 2 Score: 93.2041 bits (230), Expect = 1.896e-20 Identity = 105/400 (26.25%), Postives = 166/400 (41.50%), Query Frame = 1
Query: 892 CDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSC 2091
C + +CG+D Y N+C++++ +CK N I + R D C ++DG T C C P C K ++ PV GSD Y +EC LK+ + C++K + I + + H C+ V C PV G+DG YDN+C L +C+ + + + + F + P TC C EPCP+I++PV G+D Y++ C L AC +K + I G+ +++P C +C CP+ + + V G+DGK Y +EC +++ AC++ S L IA G C + +PV GSD + Y+NEC LK+ +C
Sbjct: 14 CPNMLDPVCGSDGKNYDNVCKLRQNACKTNTLI--TLISR-DAC-------------LQDGKPT-CVCVEP---CPKILK-PVYGSDGKNYDNECLLKLAA--------------CKSKSRILIAGSGRYHDKPTCV----CVEPCP--------EILKPVYGSDGKDYDNECLLKLAACKSKSRILI--AGFGHDKP---------------------TCVC-VEPCPEILKPVYGSDGKDYDNECLLKLAACKSKSRILIAGFGLQDDKPTC-----------VCVE----------PCPE--ILKPVYGSDGKDYDNECLLKLAACKSKSRIL-IAGFGQ-----------------------------PCPEILKPVYGSDGKDYDNECLLKLAAC 289 HSP 3 Score: 84.3445 bits (207), Expect = 1.514e-17 Identity = 59/191 (30.89%), Postives = 91/191 (47.64%), Query Frame = 1
Query: 1768 QAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSC-AENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVA 2337
+A C C +CP + + VCG+DGK Y + C +R +AC+TN++ I+ D C +P C+C C + +PV GSD + Y+NEC LK+ +C +++R++ A S G+ CVC + C ++ V G+DG Y+NEC LK AC I +A
Sbjct: 6 EAVCTCLSCPN--MLDPVCGSDGKNYDNVCKLRQNACKTNTLITLISR----DACLQDG-------------KPTCVCVEPCPKILKPVYGSDGKNYDNECLLKLAACKSKSRILIAGS----------------------GRYHDKPTCVCVEPCPEILKPVYGSDGKDYDNECLLKLAACKSKSRILIA 155 HSP 4 Score: 68.9366 bits (167), Expect = 2.708e-12 Identity = 98/406 (24.14%), Postives = 142/406 (34.98%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHAC 1878
VCGSDG YD+ C +++ C I + R QDG P CV + C + G+D Y N C +K +CK+ I GR D +C P P+ I PV GSD Y +EC LK+ +CK K + + G H C+ V C PV G+DG YDN+C L +C+ + + + ++ P TC C EPCP+I++PV G+D Y++ C L AC +K I+ G G+ CP + + V G+DGK Y +EC +++ AC
Sbjct: 21 VCGSDGKNYDNVCKLRQNACKTNTLIT-----LISRDACLQDG--------------KPTCVCV-----------EPCPKILKPVYGSDGKNYDNECLLKLAACKSKSRILIAGSGR-----------------YHDKPTCVCVEPCPE------ILKPVYGSDGKDYDNECLLKLAACKSKSRILIAGFG----------------HDKPTCV----CVEPCP--------EILKPVYGSDGKDYDNECLLKLAACKSKSRILIAGFGLQDDKP---------------------TCVC-VEPCPEILKPVYGSDGKDYDNECLLKLAACKSKS--RILIAGF----------GQPCPE----------------------ILKPVYGSDGKDYDNECLLKLAAC 289 HSP 5 Score: 62.003 bits (149), Expect = 5.422e-10 Identity = 45/120 (37.50%), Postives = 56/120 (46.67%), Query Frame = 1
Query: 1984 EPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPS 2343
E C C C + PVCGSD + Y+N C L+ +C N LIT +S C LQ KP CVC + C K+ V G+DG Y+NEC LK AC I +A S
Sbjct: 6 EAVCTC-LSCPNMLDPVCGSDGKNYDNVCKLRQNACKTNTLITLISRDAC-LQDGKP------------------TCVCVEPCPKILKPVYGSDGKNYDNECLLKLAACKSKSRILIAGS 105
BLAST of Agrin, putative vs. Ensembl Nematostella
Match: EDO38470 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCV7]) HSP 1 Score: 119.398 bits (298), Expect = 3.583e-29 Identity = 106/401 (26.43%), Postives = 161/401 (40.15%), Query Frame = 1
Query: 1132 VPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
+PVCG+D TY ++C ++ +C K +TV Y G+C++ + N AQC C + C + PVCG+DG +Y N+C + +C++Q + P + + CE CP I PVC ++ TY + C++ AC+ L ++ G A C+CP S + VCG+DGK YK +C +R ACE+ + +A+ +C K K +L C P C RPVC SD +TY N C + C ++ + V RNG C ++C K +VCG+DG Y+NEC LK C K ++V
Sbjct: 10 MPVCGTDGKTYGNKCEMRASACLKSTMVTVAYPGECESNV-------------------VNGSAQCVCPSI-CPLHY-SPVCGSDGNMYSNECAMRAAACKQQKMIT-------------------PSLPSKCSKYTQGECE---IACPDIYDPVCASNGKTYSNRCDMDADACIRDTKLTVVSQG---------------------------ALCKCPPSICSPVISPVCGSDGKIYKDDCELRKTACESKK-NIVVADKDSCSKWKRG------------YLF--CFSPRPCTADYRPVCASDGQTYPNVCTMDSAGCQKSMNLKVV---------------------------RNGTCCVVNECPKNSSKVCGSDGWTYDNECFLKLYTCRQGKDVKV 298 HSP 2 Score: 108.612 bits (270), Expect = 1.565e-25 Identity = 105/413 (25.42%), Postives = 164/413 (39.71%), Query Frame = 1
Query: 913 ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIK 2151
+CGTD TY N CEM+ +C + + Y G +CE N + C CP+ +C + PVCGSD Y +EC ++ +CK++ +T KC Q + + C + + DPVC ++G Y N+C + +C + ++ V++ + C+CP C ++ PVCG+D Y+ C L +TAC +KK + + C++ R Y C S P + VC +DG+TY + C M C+ S+ LK+ GTC C +N CP + VCGSD TY+NECFLK+ +C + + + G C + K
Sbjct: 12 VCGTDGKTYGNKCEMRASACLKSTMVTVAYPG--------ECESNVV------NGSAQCVCPS---ICPLHYS-PVCGSDGNMYSNECAMRAAACKQQKMITPSLPSKCSKYTQGEC-------------------------EIACPDIY-DPVCASNGKTYSNRCDMDADACIRDTKLTVVSQG--------------------------ALCKCPPSICSPVISPVCGSDGKIYKDDCELRKTACESKKNIVVADKDSCSKWKR---------GYLFCFSPR----------PCTADYRPVCASDGQTYPNVCTMDSAGCQ-KSMNLKVVRNGTC-------------CVVNE-------CPKN----SSKVCGSDGWTYDNECFLKLYTCRQGKDVKVQQMGECPAKISK 310 HSP 3 Score: 92.0485 bits (227), Expect = 6.110e-20 Identity = 102/423 (24.11%), Postives = 156/423 (36.88%), Query Frame = 1
Query: 655 ISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTC 1923
+ VCG+DG Y + C M+ C + V+ G +SN V N C CP+ C S +CG+D Y+N C M+ +CK + I +C K+ + G CE+ CP +I PVC S+ TY + C + ++C + LTV+ +G K ICS + PVCG+DG IY + C L K +CE + + V + + C G C P+ PC +PVC +D TY ++C + C L ++ NG C C CP++ VCG+DG TY +EC ++++ C +K+ +G C
Sbjct: 10 MPVCGTDGKTYGNKCEMRASACLKSTMVTVA-----------YPGECESN---------------VVNGSAQCVCPS-ICPLHYSPVCGSDGNMYSNECAMRAAACKQQKMITPSLPSKCS--KYTQ----GECEIA---------CP--------DIYDPVCASNGKTYSNRCDMDADACIRDTKLTVVSQGA-LCKCPPSICSPVI-----------------------------SPVCGSDGKIYKDDCELRKTACESKKNIVVADKDSCSKW-----------------KRGYLFCFSPR-PCTADYRPVCASDGQTYPNVCTMDSAGCQKSMNLKVVRNGTC---------------------------CVVNECPKN--SSKVCGSDGWTYDNECFLKLYTCRQGK-DVKVQQMGEC 304 HSP 4 Score: 82.8037 bits (203), Expect = 7.789e-17 Identity = 63/200 (31.50%), Postives = 92/200 (46.00%), Query Frame = 1
Query: 1819 VCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITA--------VSEGPCKLQ---FIKP---------------DEERCPEDCPLGKMCRNGQCVCTDK-CSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVA 2337
VCGTDGKTY ++C MR AC S + +A G C+ ++ + QC+CP+ C PVCGSD Y NEC ++ +C + ++IT ++G C++ P D + C D L + + C C CS V VCG+DG Y+++CEL++ AC K I VA
Sbjct: 12 VCGTDGKTYGNKCEMRASAC-LKSTMVTVAYPGECES---------NVVNGSA----QCVCPSICPLHYSPVCGSDGNMYSNECAMRAAACKQQKMITPSLPSKCSKYTQGECEIACPDIYDPVCASNGKTYSNRCDMDADACIRDTKLTVVSQGALCKCPPSICSPVISPVCGSDGKIYKDDCELRKTACESKKNIVVA 197 HSP 5 Score: 75.8702 bits (185), Expect = 1.584e-14 Identity = 84/332 (25.30%), Postives = 121/332 (36.45%), Query Frame = 1
Query: 1369 PVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVC--TDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCI 2358
PVCG DG Y NKC + +C K + V CE+N NG + C CP CP PVCG+D Y + C + AC K+Q PS S Q +CE CP + + VC ++GKTY + C M AC ++ L + + G KC P+ C V PVCGSD + Y+++C L+ +C + I + C + G C C+ VC +DG Y N C + C + ++V + C+
Sbjct: 11 PVCGTDGKTYGNKCEMRASACLKSTMVTVAYPGECESNVV----------------NGSAQCVCPSI-CPLHYSPVCGSDGNMYSNECAMRAAAC--------------------KQQKMITPSLPSKCSKYTQGECEI-ACPD--IYDPVCASNGKTYSNRCDMDADACIRDT-KLTVVSQGALCKCP----------------------PSICSPVISPVCGSDGKIYKDDCELRKTACESKKNIVVADKDSCSKW-------------------KRGYLFCFSPRPCTADYRPVCASDGQTYPNVCTMDSAGCQKSMNLKVVRNGTCCV 260 HSP 6 Score: 67.0106 bits (162), Expect = 1.478e-11 Identity = 36/110 (32.73%), Postives = 52/110 (47.27%), Query Frame = 1
Query: 1999 CPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTI 2328
CP C Y PVCG+D +TY N+C ++ +C ++ ++T G C+ + + QCVC C VCG+DGN Y NEC ++ AC K I
Sbjct: 1 CPIFCTYEYMPVCGTDGKTYGNKCEMRASACLKSTMVTVAYPGECESNVVN----------------GSAQCVCPSICPLHYSPVCGSDGNMYSNECAMRAAACKQQKMI 94
BLAST of Agrin, putative vs. Ensembl Nematostella
Match: EDO40383 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7E2]) HSP 1 Score: 115.931 bits (289), Expect = 3.227e-28 Identity = 94/345 (27.25%), Postives = 145/345 (42.03%), Query Frame = 1
Query: 892 CDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCEC-PTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTC 1923
C + +CG+D TY N+C + C++ FI + + G C C DG G IC+CP ++ PVCG D TY +EC L++ESC ++ S+T + G C ++ A+C CD + DP+CG DG Y+N L +C +Q + + C + CPDP CP + VCG D +TY+++C L +C +P+ N + +C G+ Y + +CEC TCP+S + VCG+D + Y +EC ++ AC L + N G C
Sbjct: 9 CTREYAPVCGSDGNTYNNLCLLTAARCQSQTFIYRAHFGTCGT----------RCVPSADGSGHICECPRS----CPSVNYPVCGDDGQTYDNECLLQLESCSRRRSITTVNYGSCG----------------------ADNYARCECDLR--PDPAYDPICGTDGKTYNNDKDLESAACAQQTSIVRWHKGPCTVG--AVCTCPDP------------------AACPLVKSRVCGTDGITYDNLCRLRAESCRRYQPV--------NVKVKCAFYGQCVWYYD------GRTQCECRRTCPRS--DQLVCGSDDRDYANECVLQARACTWRDSLLTVHNKGPC 279 HSP 2 Score: 110.153 bits (274), Expect = 3.173e-26 Identity = 99/345 (28.70%), Postives = 140/345 (40.58%), Query Frame = 1
Query: 1132 VPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQ-CSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECP---TCPQSGLGESVCGTDGKTYKSECHMRMHACET-NSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCA-ENRLITAVSEGPCKLQFI 2148
PVCGSD TY + C L C+ + + + G C G +C+ +++ C C C + PVCG+DG YDN+C L SC ++ + +N Y C DN + CEC P P P+CG D TY + +L AC + + + G C V A C CP CP + VCGTDG TY + C +R +C + +K+ KC+FY C QC C C + VCGSDDR Y NEC L+ ++C + L+T ++GPC + I
Sbjct: 14 APVCGSDGNTYNNLCLLTAARCQSQTFIYRAHFGTC----------------GTRCVPSADGSGHICECP-RSCPSV-NYPVCGDDGQTYDNECLLQLESCSRRRSITTVN-----------------YGSCGADNYAR--CECDLRPDPAY-DPICGTDGKTYNNDKDLESAACAQQTSIVRWHKGPCT----------------------VGAVCTCPDPAACPL--VKSRVCGTDGITYDNLCRLRAESCRRYQPVNVKV------------KCAFYGQCVWYYDGRTQCECRRTCPRSDQLVCGSDDRDYANECVLQARACTWRDSLLTVHNKGPCGGRRI 284 HSP 3 Score: 88.5817 bits (218), Expect = 8.021e-19 Identity = 74/257 (28.79%), Postives = 117/257 (45.53%), Query Frame = 1
Query: 1573 CPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECP-TCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIK--PDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRV 2334
C + PVCG+D TY ++C LT C ++ ++ + G C RC + +D CECP +CP + VCG DG+TY +EC +++ +C + ++ N G+C Y+ C+ + +P P+CG+D +TY N+ L+ +CA+ I +GPC + + PD P CPL VK RVCGTDG Y+N C L+ ++C + + V
Sbjct: 9 CTREYAPVCGSDGNTYNNLCLLTAARCQSQTFIYRAHFGTCGT--RC-----------VPSADGSGHICECPRSCPS--VNYPVCGDDGQTYDNECLLQLESC-SRRRSITTVNYGSCGA------DNYARCECDLRPDP----------AYDPICGTDGKTYNNDKDLESAACAQQTSIVRWHKGPCTVGAVCTCPD----PAACPL-----------------VKSRVCGTDGITYDNLCRLRAESCRRYQPVNV 212 HSP 4 Score: 74.3294 bits (181), Expect = 4.295e-14 Identity = 66/221 (29.86%), Postives = 99/221 (44.80%), Query Frame = 1
Query: 661 VCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQ-----------------DGISKSNEQFCQHQSCPPYATCVT------NPYPYCKCPND-KCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKIS-LTVMYKGKCQTK 1248
VCG DG YD+ CL++ + C ++SI CG + DG + +N++ + +C + V C CP+ C S++CGTD ITY N+C ++ SC+ + + + VKC F G C DG T C+C C ++ Q+ VCGSDD Y +EC L+ +C + S LTV KG C +
Sbjct: 77 VCGDDGQTYDNECLLQLESCSRRRSITTVNYGSCGADNYARCECDLRPDPAYDPICGTDGKTYNNDKDLESAACAQQTSIVRWHKGPCTVGAVCTCPDPAACPLVKSRVCGTDGITYDNLCRLRAESCRRYQPVNVK----------VKCAFYGQCVWYYDGR-TQCEC---RRTCPRSDQL-VCGSDDRDYANECVLQARACTWRDSLLTVHNKGPCGGR 282 HSP 5 Score: 55.8398 bits (133), Expect = 5.478e-8 Identity = 36/113 (31.86%), Postives = 47/113 (41.59%), Query Frame = 1
Query: 1990 QCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTI 2328
+C+C C PVCGSD TY N C L C I G C + + P G +C C C V VCG DG Y+NEC L+ ++C ++I
Sbjct: 2 KCVCSAACTREYAPVCGSDGNTYNNLCLLTAARCQSQTFIYRAHFGTCGTRCV-------PSADGSGHICE-----CPRSCPSVNYPVCGDDGQTYDNECLLQLESCSRRRSI 102
BLAST of Agrin, putative vs. Ensembl Medaka
Match: agrn (agrin [Source:NCBI gene;Acc:101156494]) HSP 1 Score: 316.62 bits (810), Expect = 5.754e-87 Identity = 249/767 (32.46%), Postives = 358/767 (46.68%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQ-ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIR------------VAPSVI-----------GCISHNYIADEKNKRS-----CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC 2676
D C + C FGA C++ P +P A C C C + VCGSD Y + C M+R +C Q+ I+V C K+ C +C +TCV + C CP CD + + +CG+D Y + CE+ + +CK + I Q++G CD CK + N +C + + L +P E C P+C SD TY +ECH+ +K I L ++ G+C+ + D C + +C + A C+ +CSCD L+C +T++ P+CG +G Y N C K CE + + + + C+ N PC C C + NN + CECP E C PVCG+D +Y S C + C +K + I + G+C+E +C IC DL +C CP+ C +S + VCG+DG TY SEC + + AC T L++ + G C C C++ ++C N+ QC+ P+CGSD +TY+NEC L+ SC +N+ I +G C E C + CR + +CVC C V VCG+DG Y NECELK+ C + + V P+ + GC S N A ++ C CN GS CDP+T QC CK G+ GQ CDRC G+WNF TEN GCT CSC+ GS C +G C+CK G C+VCP G + +GC
Sbjct: 67 DGCRGMLCGFGAVCERDPADPAKADCVCKKGDCPS--LVAPVCGSDSSTYSNECEMERAQCKAQRRIKVLRRGPCSLKDP------------CAEVTCSYGSTCVQSSDGLAAKCMCPLG-CDGKAGETVCGSDGNDYPSECELHQHACKNQKNIRVQHLGPCDPCKDAENSLNVLCRV--EALTRRPLLYSPPESCPPGDD-PLCASDGHTYPNECHMTATGVQKGIKLQKIHAGQCR---RLDECQD-ECLFNAVCVVEPEG-PRCSCDPLECDDTYK-PLCGRNGRTYTNDCLRRKAECEMKTLISIRSQGPCDLNTPSPCLDKVCKH-GAVCVVKNN-EPVCECP-EACQLTSDPVCGSDGHSYGSPCEMRAMGCAFQKTIHIQHKGLCDE------ACANCSFGAIC--DLQSKRCVCPSECVKSR--QPVCGSDGNTYDSECELHVKAC-TQQTDLQVVSQGACKTCGPTVCAWGALCVQNKCECQQCV-----GEALSPLCGSDGKTYDNECELRRSSCLQNKKIDVEKDGSCDEDCGSGGSGSGAESCEQDR-CRKYGGTWDEDAEDDRCVCDFSCESVPHNAVCGSDGKNYSNECELKKAGCDRRELVSIQNHGPCAALSAVTPTALTAAQHCSQSAHGCCSDNATAALGAGQAGCPSTCQCNLYGSYKGTCDPATGQCSCKPGVGGQKCDRCEPGFWNFRGIVTENMSGCTPCSCDPVGSIRDDCEQMSGLCSCKTAVKGLKCNVCPDGSKMGMNGC 789 HSP 2 Score: 142.124 bits (357), Expect = 2.504e-33 Identity = 163/716 (22.77%), Postives = 298/716 (41.62%), Query Frame = 1
Query: 2725 FLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP----NCPHIDFKNSVDVIEDPDKEEQSEKENLK------------IKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESL---QLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKH--ENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKR--------DKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ +SY + + Y ++ I++ +S+ G++ Y+ S D++++ L N VE R + GSG L S+V+++ G H + V R R+ L +DG G +P L+ IG T + ++ V+ G VGC+ L + ++ + + + S V C KN + +++E K ++D C K C CQ E Y C C G +G CE+V +++ F +S++ L LRQ++ + + + ++GL+ + D FIS+ LN LE G + S + WN + + ++ + + +N QP + V+S ++ K + +F GG ++ + L F G +QKI++ + + + + + +N+ +QG PC Q PC G C P + C+C P + +N + H+ A + G++ +Y K +K+ N + +T + G+L+ K +AI +G +++ +LG + VLR + ++ N+W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GCI ++ VD + +LV DA N IL C
Sbjct: 1274 FIPSFGGQSYLAFPTMKAYHTVRIAMAFRASEMNGVLLYNGQRG-SKDFISLTLVNGKVELRFNTGSGTGTLTSKVKVSQGHWHQVEVTRNRRNGVLSVDGEPDVQGESPHGTDGLNLDTELFIGGVTEDLKQDVTARTGVSAGLVGCIRMLDVN----NRMLNLQEDGGDSLFGSGVGECGNNPCEPNPCKNGAQC-----QVKEAEMFQCKCSKGFWGTLCADVRDPCAASK-CHATSQCQVLPEGGYKCVCPMGREGRHCEKVAERRGAYMPTFNGDSYLELKGLHLYGHDLRQKVSMMVVLMANDSDGLIFYNGQKTDGKGDFISLGLNNGILEFRYDLGKGPAIIRSKAPLPLKVWNTINLERSLRKGEIRINDQ-QPARGE-----SPAVHSDLNLK--------ESLFVGGA--PDYSKLARAAGLTEGFKGTVQKIMLMSDPVLKEENALSSSNVAMFQGHPCS--QEPCQN----GGRCNPMLATYECSCFPGYVGDNCSV---AIHEKSAGETEAVAFDGRTFIEYHNGVTKSQLTNEIPDEKALLVNKFELSIRTEATHGLLLWSGKGVERSDYI-ALAIVDGRVQMTYDLGSRPVVLR--SSVRVNTNRWIHIKASRALRDGFLQVGNE---AAVTGSSPLAATQLDTDGALWLGGLEELAVARRLPKAYSTGFVGCIKDVVVDGVELHLVEDAL-NSPKILHC 1946 HSP 3 Score: 110.153 bits (274), Expect = 1.425e-23 Identity = 147/584 (25.17%), Postives = 221/584 (37.84%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-------TKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP-----YQYCR------IDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DC-VYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCP 2172
PC CK GA C P VC C CQ VCGSDG Y S C M+ C QK+I + +C A + S C QS C CP++ C S +CG+D TY + CE+ +C ++ G C C C + +C + C+C + C P+CGSD TY +EC L+ SC + + V G C + ++ C +C +C CD+ C++ + VCG+DG Y N+C L K C+++ + + N + PC+ + P Q+C +N + + CP Q N +Y+ C+ C K +P + + GI E C G DC + + C C T + GL +VC K M M+ C++ A C + C F + C + + C CP+ DC V + VCGSD TY ++C LK +C +++ I G C +P + P
Sbjct: 365 PCLDKVCKHGAVCVVKNNEP---VCECPEA-CQLTSD--PVCGSDGHSYGSPCEMRAMGCAFQKTIHIQHKGLCDEACA-----NCSFGAICDLQS------------KRCVCPSE-CVKSRQPVCGSDGNTYDSECELHVKACTQQTDLQVVSQGACKTCGPTVCAWGALC------VQNKCEC----QQCVGEALSPLCGSDGKTYDNECELRRSSCLQNKKIDVEKDGSCDEDCGSGGSGSGAESCEQDRCRKYGGTWDEDAEDDRCVCDF-SCESVPHNAVCGSDGKNYSNECELKKAGCDRRELVSIQN-----HGPCAALSAVTPTALTAAQHCSQSAHGCCSDNATAALGAGQAGCPSTCQ---CNLYGSYKGTCDPATGQCSCKPGVGGQKCDRCEPGFWNFRGIVTENMSGCTPCSCDPVGSIRDDC--------EQMSGLCSCKTAVK-GLKCNVCPDGSK-------MGMNGCDSGPEAP--------GSCADLVCGFGATCIVVNG-QAHCECPSPDCDVKNKTKVCGSDGVTYADQCQLKTIACRQDKDILVAHTGQCTESIFEPADPHPP 880 HSP 4 Score: 88.5817 bits (218), Expect = 5.200e-17 Identity = 100/455 (21.98%), Postives = 179/455 (39.34%), Query Frame = 1
Query: 2494 RCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAK----SYWSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIF-------------PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNH 3783
+C G+W T C+ R + C+ + C+ L G Y VCP+G G + E + A F DS+ + + + +S+ + L+ SDG+IFY+ TD D++++ L N +E+R DLG G ++S+ L + ++I +ER R + I+ G +P ++L+ +G A P + L+ +T+GF G + KI LM+ P + +N++ S + + + +PC NGG C Y C C PGY G C + + F +F+ + P E L K E++ A +GLL + G +R+ +I++ + +++T L S +W H+ +A + +L + +
Sbjct: 1476 KCSKGFWG------TLCADVRDPCAASKCHATS---QCQVLPEGGYKCVCPMG----REGRHCEKVAERR-GAYMPTFNGDSYLELKGLHLYGHDLRQKVSMMVVLMANDSDGLIFYNGQKTDGKGDFISLGLNNGILEFRYDLGKGPAIIRSKAPLPLKVWNTINLERSLRKGEIRINDQQPARGESPAVHSDLNLKESLFVGGA--PDYSKLARAAGLTEGFKGTVQ----------KIMLMSD---------------PVLKEENAL-----------SSSNVAMFQGHPCSQEPCQNGGRCNPML-ATYECSCFPGYVGDNCSVAIHEKSAGETEAVAFDGRTFIEYHNGVTKSQLTNEIPDEKALLVN--KFELSIRTEATHGLLLWSGKGVERS-DYIALAIVDGRVQMTYDLGSRPVVLRSSVRVNTNRWIHIKASRALRDGFLQVGN 1874 HSP 5 Score: 69.707 bits (169), Expect = 3.225e-11 Identity = 58/251 (23.11%), Postives = 107/251 (42.63%), Query Frame = 1
Query: 3340 KIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ---NSDIILNNITQWQGPPCGPLQSPC 4080
K + C +H PCL+GG C+ YSC+C G G CE+V+ + I F S++ FP+ ++ +++ + F NG+L + +G + FIS+TL +E+ T G L S G W+ V + + + L+++ P + LN+++ +F GGV + + + G I+ + +N+ + Q + + + + PC P +PC
Sbjct: 1231 KTQRPCDSH-PCLHGGTCEDDGS-TYSCRCPAGRGGPVCEKVMKYFIPSFGGQSYLAFPT--MKAYHTVRIAMAFRASEMNGVLLY--NGQRGSKDFISLTLVNGKVELRFNTGSGTGTLTSKVKVSQGHWHQVEVTRNRRNGVLSVDGEPDVQGESPHGTDGLNLDT--------------ELFIGGVTEDLKQDVTARTGVSAGLVGCIRMLDVNNRMLNLQEDGGDSLFGSGVGECGNNPCEP--NPC 1459
BLAST of Agrin, putative vs. Ensembl Medaka
Match: agrn (agrin [Source:NCBI gene;Acc:101156494]) HSP 1 Score: 316.235 bits (809), Expect = 6.516e-87 Identity = 248/766 (32.38%), Postives = 357/766 (46.61%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQ-ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNG----------QCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIR------------VAPSVI-----------GCISHNYIADEKNKRS-----CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC 2676
D C + C FGA C++ P +P A C C C + VCGSD Y + C M+R +C Q+ I+V C K+ C +C +TCV + C CP CD + + +CG+D Y + CE+ + +CK + I Q++G CD CK + N +C + + L +P E C P+C SD TY +ECH+ +K I L ++ G+C+ + D C + +C + A C+ +CSCD L+C +T++ P+CG +G Y N C K CE + + + + C+ N PC C C + NN + CECP E C PVCG+D +Y S C + C +K + I + G+C+E +C IC DL +C CP+ C +S + VCG+DG TY SEC + + AC T L++ + G C C C++ ++C N+ QC+ P+CGSD +TY+NEC L+ SC +N+ I +G C E C + + G +CVC C V VCG+DG Y NECELK+ C + + V P+ + GC S N A ++ C CN GS CDP+T QC CK G+ GQ CDRC G+WNF TEN GCT CSC+ GS C +G C+CK G C+VCP G + +GC
Sbjct: 67 DGCRGMLCGFGAVCERDPADPAKADCVCKKGDCPS--LVAPVCGSDSSTYSNECEMERAQCKAQRRIKVLRRGPCSLKDP------------CAEVTCSYGSTCVQSSDGLAAKCMCPLG-CDGKAGETVCGSDGNDYPSECELHQHACKNQKNIRVQHLGPCDPCKDAENSLNVLCRV--EALTRRPLLYSPPESCPPGDD-PLCASDGHTYPNECHMTATGVQKGIKLQKIHAGQCR---RLDECQD-ECLFNAVCVVEPEG-PRCSCDPLECDDTYK-PLCGRNGRTYTNDCLRRKAECEMKTLISIRSQGPCDLNTPSPCLDKVCKH-GAVCVVKNN-EPVCECP-EACQLTSDPVCGSDGHSYGSPCEMRAMGCAFQKTIHIQHKGLCDE------ACANCSFGAIC--DLQSKRCVCPSECVKSR--QPVCGSDGNTYDSECELHVKAC-TQQTDLQVVSQGACKTCGPTVCAWGALCVQNKCECQQCV-----GEALSPLCGSDGKTYDNECELRRSSCLQNKKIDVEKDGSCDEDCGSGGSGSGAESCEQDRCRKYGGTWDEDAEDDRCVCDFSCESVPHNAVCGSDGKNYSNECELKKAGCDRRELVSIQNHGPCAALSAVTPTALTAAQHCSQSAHGCCSDNATAALGAGQAGCPSTCQCNLYGSYKGTCDPATGQCSCKPGVGGQKCDRCEPGFWNFRGIVTENMSGCTPCSCDPVGSIRDDCEQMSGLCSCKTAVKGLKCNVCPDGSKMGMNGC 789 HSP 2 Score: 144.436 bits (363), Expect = 5.840e-34 Identity = 162/716 (22.63%), Postives = 296/716 (41.34%), Query Frame = 1
Query: 2725 FLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP----NCPHIDFKNSVDVIEDPDKEEQSEKENLK------------IKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESL---QLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKH--ENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKR--------DKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ +SY + + Y ++ I++ +S+ G++ Y+ S D++++ L N VE R + GSG L S+V+++ G H + V R R+ L +DG G +P L+ IG T + ++ V+ G VGC+ L + ++ + + + S V C KN + +++E K ++D C K C CQ E Y C C G +G CE+V +++ F +S++ L LRQ++ + + + ++GL+ + D FIS+ LN LE G + S + WN + + ++ + + +N QP + P ++N + +F GG ++ + L F G +QKI++ + + + + + +N+ +QG PC Q PC G C P + C+C P + +N + H+ A + G++ +Y K +K+ N + +T + G+L+ K +AI +G +++ +LG + VLR + ++ N+W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GCI ++ VD + +LV DA N IL C
Sbjct: 1143 FIPSFGGQSYLAFPTMKAYHTVRIAMAFRASEMNGVLLYNGQRG-SKDFISLTLVNGKVELRFNTGSGTGTLTSKVKVSQGHWHQVEVTRNRRNGVLSVDGEPDVQGESPHGTDGLNLDTELFIGGVTEDLKQDVTARTGVSAGLVGCIRMLDVN----NRMLNLQEDGGDSLFGSGVGECGNNPCEPNPCKNGAQC-----QVKEAEMFQCKCSKGFWGTLCADVRDPCAASK-CHATSQCQVLPEGGYKCVCPMGREGRHCEKVAERRGAYMPTFNGDSYLELKGLHLYGHDLRQKVSMMVVLMANDSDGLIFYNGQKTDGKGDFISLGLNNGILEFRYDLGKGPAIIRSKAPLPLKVWNTINLERSLRKGEIRINDQ-QPARGE-SPAVHSDLN------------LKESLFVGGA--PDYSKLARAAGLTEGFKGTVQKIMLMSDPVLKEENALSSSNVAMFQGHPCS--QEPCQN----GGRCNPMLATYECSCFPGYVGDNCSV---AIHEKSAGETEAVAFDGRTFIEYHNGVTKSQLTNEIPDEKALLVNKFELSIRTEATHGLLLWSGKGVERSDYI-ALAIVDGRVQMTYDLGSRPVVLR--SSVRVNTNRWIHIKASRALRDGFLQVGNE---AAVTGSSPLAATQLDTDGALWLGGLEELAVARRLPKAYSTGFVGCIKDVVVDGVELHLVEDAL-NSPKILHC 1815 HSP 3 Score: 106.686 bits (265), Expect = 1.691e-22 Identity = 145/571 (25.39%), Postives = 217/571 (38.00%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-------TKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP-----YQYCR------IDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DC-VYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPC 2133
PC CK GA C P VC C CQ VCGSDG Y S C M+ C QK+I + +C A + S C QS C CP++ C S +CG+D TY + CE+ +C ++ G C C C + +C + C+C + C P+CGSD TY +EC L+ SC + + V G C + ++ C +C +C CD+ C++ + VCG+DG Y N+C L K C+++ + + N + PC+ + P Q+C +N + + CP Q N +Y+ C+ C K +P + + GI E C G DC + + C C T + GL +VC K M M+ C++ A C + C F + C + + C CP+ DC V + VCGSD TY ++C LK +C +++ I G C
Sbjct: 365 PCLDKVCKHGAVCVVKNNEP---VCECPEA-CQLTSD--PVCGSDGHSYGSPCEMRAMGCAFQKTIHIQHKGLCDEACA-----NCSFGAICDLQS------------KRCVCPSE-CVKSRQPVCGSDGNTYDSECELHVKACTQQTDLQVVSQGACKTCGPTVCAWGALC------VQNKCEC----QQCVGEALSPLCGSDGKTYDNECELRRSSCLQNKKIDVEKDGSCDEDCGSGGSGSGAESCEQDRCRKYGGTWDEDAEDDRCVCDF-SCESVPHNAVCGSDGKNYSNECELKKAGCDRRELVSIQN-----HGPCAALSAVTPTALTAAQHCSQSAHGCCSDNATAALGAGQAGCPSTCQ---CNLYGSYKGTCDPATGQCSCKPGVGGQKCDRCEPGFWNFRGIVTENMSGCTPCSCDPVGSIRDDC--------EQMSGLCSCKTAVK-GLKCNVCPDGSK-------MGMNGCDSGPEAP--------GSCADLVCGFGATCIVVNG-QAHCECPSPDCDVKNKTKVCGSDGVTYADQCQLKTIACRQDKDILVAHTGQC 867 HSP 4 Score: 89.3521 bits (220), Expect = 3.159e-17 Identity = 100/455 (21.98%), Postives = 179/455 (39.34%), Query Frame = 1
Query: 2494 RCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAK----SYWSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIF-------------PSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNH 3783
+C G+W T C+ R + C+ + C+ L G Y VCP+G G + E + A F DS+ + + + +S+ + L+ SDG+IFY+ TD D++++ L N +E+R DLG G ++S+ L + ++I +ER R + I+ G +P ++L+ +G A P + L+ +T+GF G + KI LM+ P + +N++ S + + + +PC NGG C Y C C PGY G C + + F +F+ + P E L K E++ A +GLL + G +R+ +I++ + +++T L S +W H+ +A + +L + +
Sbjct: 1345 KCSKGFWG------TLCADVRDPCAASKCHATS---QCQVLPEGGYKCVCPMG----REGRHCEKVAERR-GAYMPTFNGDSYLELKGLHLYGHDLRQKVSMMVVLMANDSDGLIFYNGQKTDGKGDFISLGLNNGILEFRYDLGKGPAIIRSKAPLPLKVWNTINLERSLRKGEIRINDQQPARGESPAVHSDLNLKESLFVGGA--PDYSKLARAAGLTEGFKGTVQ----------KIMLMSD---------------PVLKEENAL-----------SSSNVAMFQGHPCSQEPCQNGGRCNPML-ATYECSCFPGYVGDNCSVAIHEKSAGETEAVAFDGRTFIEYHNGVTKSQLTNEIPDEKALLVN--KFELSIRTEATHGLLLWSGKGVERS-DYIALAIVDGRVQMTYDLGSRPVVLRSSVRVNTNRWIHIKASRALRDGFLQVGN 1743 HSP 5 Score: 70.4774 bits (171), Expect = 1.722e-11 Identity = 58/251 (23.11%), Postives = 107/251 (42.63%), Query Frame = 1
Query: 3340 KIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ---NSDIILNNITQWQGPPCGPLQSPC 4080
K + C +H PCL+GG C+ YSC+C G G CE+V+ + I F S++ FP+ ++ +++ + F NG+L + +G + FIS+TL +E+ T G L S G W+ V + + + L+++ P + LN+++ +F GGV + + + G I+ + +N+ + Q + + + + PC P +PC
Sbjct: 1100 KTQRPCDSH-PCLHGGTCEDDGS-TYSCRCPAGRGGPVCEKVMKYFIPSFGGQSYLAFPT--MKAYHTVRIAMAFRASEMNGVLLY--NGQRGSKDFISLTLVNGKVELRFNTGSGTGTLTSKVKVSQGHWHQVEVTRNRRNGVLSVDGEPDVQGESPHGTDGLNLDT--------------ELFIGGVTEDLKQDVTARTGVSAGLVGCIRMLDVNNRMLNLQEDGGDSLFGSGVGECGNNPCEP--NPC 1328
BLAST of Agrin, putative vs. Ensembl Medaka
Match: agrn (agrin [Source:NCBI gene;Acc:101156494]) HSP 1 Score: 313.538 bits (802), Expect = 5.383e-86 Identity = 247/760 (32.50%), Postives = 355/760 (46.71%), Query Frame = 1
Query: 559 CKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNP---YPYCKCPNDKCDSSSSQ-ICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENN---PCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPT-CPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCR-----------NGQCVCTDKCSKVKLR-VCGTDGNYYENECELKRKACIDNKTIR------------VAPSVI-----------GCISHNYIADEKNKRS-----CLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNF----TEN--GCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGC 2676
C FGA C++ P +P A C C C + VCGSD Y + C M+R +C Q+ I+V C K+ C +C +TCV + C CP CD + + +CG+D Y + CE+ + +CK + I Q++G CD CK + N +C + + L +P E C P+C SD TY +ECH+ +K I L ++ G+C+ + D C + +C + A C+ +CSCD L+C +T++ P+CG +G Y N C K CE + + + + C+ N PC C C + NN + CECP E C PVCG+D +Y S C + C +K + I + G+C+E +C IC DL +C CP+ C +S + VCG+DG TY SEC + + AC T L++ + G C C C++ ++C N+ QC+ P+CGSD +TY+NEC L+ SC +N+ I +G C E C + CR + +CVC C V VCG+DG Y NECELK+ C + + V P+ + GC S N A ++ C CN GS CDP+T QC CK G+ GQ CDRC G+WNF TEN GCT CSC+ GS C +G C+CK G C+VCP G + +GC
Sbjct: 3 CGFGAVCERDPADPAKADCVCKKGDCPS--LVAPVCGSDSSTYSNECEMERAQCKAQRRIKVLRRGPCSLKDP------------CAEVTCSYGSTCVQSSDGLAAKCMCPLG-CDGKAGETVCGSDGNDYPSECELHQHACKNQKNIRVQHLGPCDPCKDAENSLNVLCRV--EALTRRPLLYSPPESCPPGDD-PLCASDGHTYPNECHMTATGVQKGIKLQKIHAGQCR---RLDECQD-ECLFNAVCVVEPEG-PRCSCDPLECDDTYK-PLCGRNGRTYTNDCLRRKAECEMKTLISIRSQGPCDLNTPSPCLDKVCKH-GAVCVVKNN-EPVCECP-EACQLTSDPVCGSDGHSYGSPCEMRAMGCAFQKTIHIQHKGLCDE------ACANCSFGAIC--DLQSKRCVCPSECVKSR--QPVCGSDGNTYDSECELHVKAC-TQQTDLQVVSQGACKTCGPTVCAWGALCVQNKCECQQCV-----GEALSPLCGSDGKTYDNECELRRSSCLQNKKIDVEKDGSCDEDCGSGGSGSGAESCEQDR-CRKYGGTWDEDAEDDRCVCDFSCESVPHNAVCGSDGKNYSNECELKKAGCDRRELVSIQNHGPCAALSAVTPTALTAAQHCSQSAHGCCSDNATAALGAGQAGCPSTCQCNLYGSYKGTCDPATGQCSCKPGVGGQKCDRCEPGFWNFRGIVTENMSGCTPCSCDPVGSIRDDCEQMSGLCSCKTAVKGLKCNVCPDGSKMGMNGC 718 HSP 2 Score: 150.214 bits (378), Expect = 9.998e-36 Identity = 163/708 (23.02%), Postives = 298/708 (42.09%), Query Frame = 1
Query: 2725 FLKDSFAKSYWS-GNAENYLSINISLVPYSSD--GIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKP----NCPHIDFKNSVDVIEDPDKEEQSEKENLK------------IKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV----SHIWKFKDNSFVIFPSESL---QLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKH--ENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNT--LATDGWMWLGGAKK--PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
F+ +SY + + Y ++ I++ +S+ G++ Y+ S D++++ L N VE R + GSG L S+V+++ G H + V R R+ L +DG G +P L+ IG T + ++ V+ G VGC+ L + ++ + + + S V C KN + +++E K ++D C K C CQ E Y C C G +G CE+V +++ F +S++ L LRQ++ + + + ++GL+ + D FIS+ LN LE G + S + WN + + ++ + + +N QP + V+S ++ K + +F GG ++ + L F G +QKI++ + + + + + +N+ +QG PC Q PC G C P + C+C P + +N + H+ A + G++ +Y K +K+ N + +T + G+L+ K +AI +G +++ +LG + VLR + ++ N+W ++ R R+ L + + A + ++ L TDG +WLGG ++ P Y+T F GCI ++ VD + +LV DA N IL C
Sbjct: 1203 FIPSFGGQSYLAFPTMKAYHTVRIAMAFRASEMNGVLLYNGQRG-SKDFISLTLVNGKVELRFNTGSGTGTLTSKVKVSQGHWHQVEVTRNRRNGVLSVDGEPDVQGESPHGTDGLNLDTELFIGGVTEDLKQDVTARTGVSAGLVGCIRMLDVN----NRMLNLQEDGGDSLFGSGVGECGNNPCEPNPCKNGAQC-----QVKEAEMFQCKCSKGFWGTLCADVRDPCAASK-CHATSQCQVLPEGGYKCVCPMGREGRHCEKVAERRGAYMPTFNGDSYLELKGLHLYGHDLRQKVSMMVVLMANDSDGLIFYNGQKTDGKGDFISLGLNNGILEFRYDLGKGPAIIRSKAPLPLKVWNTINLERSLRKGEIRINDQ-QPARGE-----SPAVHSDLNLK--------ESLFVGGA--PDYSKLARAAGLTEGFKGTVQKIMLMSDPVLKEENALSSSNVAMFQGHPCS--QEPCQN----GGRCNPMLATYECSCFPGYVGDNCSV---AIHEKSAGETEAVAFDGRTFIEYHNGVTKSEKALLVNKFELSIRTEATHGLLLWSGKGVERSDYI-ALAIVDGRVQMTYDLGSRPVVLR--SSVRVNTNRWIHIKASRALRDGFLQVGNE---AAVTGSSPLAATQLDTDGALWLGGLEELAVARRLPKAYSTGFVGCIKDVVVDGVELHLVEDAL-NSPKILHC 1867 HSP 3 Score: 109.768 bits (273), Expect = 1.795e-23 Identity = 147/584 (25.17%), Postives = 221/584 (37.84%), Query Frame = 1
Query: 541 PCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQ-------TKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDP-----YQYCR------IDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTK-----------KPLWIIYNGICNEEPR------CKEQGK---DCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPT-DC-VYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCP 2172
PC CK GA C P VC C CQ VCGSDG Y S C M+ C QK+I + +C A + S C QS C CP++ C S +CG+D TY + CE+ +C ++ G C C C + +C + C+C + C P+CGSD TY +EC L+ SC + + V G C + ++ C +C +C CD+ C++ + VCG+DG Y N+C L K C+++ + + N + PC+ + P Q+C +N + + CP Q N +Y+ C+ C K +P + + GI E C G DC + + C C T + GL +VC K M M+ C++ A C + C F + C + + C CP+ DC V + VCGSD TY ++C LK +C +++ I G C +P + P
Sbjct: 294 PCLDKVCKHGAVCVVKNNEP---VCECPEA-CQLTSD--PVCGSDGHSYGSPCEMRAMGCAFQKTIHIQHKGLCDEACA-----NCSFGAICDLQS------------KRCVCPSE-CVKSRQPVCGSDGNTYDSECELHVKACTQQTDLQVVSQGACKTCGPTVCAWGALC------VQNKCEC----QQCVGEALSPLCGSDGKTYDNECELRRSSCLQNKKIDVEKDGSCDEDCGSGGSGSGAESCEQDRCRKYGGTWDEDAEDDRCVCDF-SCESVPHNAVCGSDGKNYSNECELKKAGCDRRELVSIQN-----HGPCAALSAVTPTALTAAQHCSQSAHGCCSDNATAALGAGQAGCPSTCQ---CNLYGSYKGTCDPATGQCSCKPGVGGQKCDRCEPGFWNFRGIVTENMSGCTPCSCDPVGSIRDDC--------EQMSGLCSCKTAVK-GLKCNVCPDGSK-------MGMNGCDSGPEAPG--------SCADLVCGFGATCIVVNG-QAHCECPSPDCDVKNKTKVCGSDGVTYADQCQLKTIACRQDKDILVAHTGQCTESIFEPADPHPP 809 HSP 4 Score: 92.4337 bits (228), Expect = 3.267e-18 Identity = 98/445 (22.02%), Postives = 180/445 (40.45%), Query Frame = 1
Query: 2494 RCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAK----SYWSGNAENYLSINISLVPYSSDGIIFYHSD-TDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVV-------SHIWKFKDNSFVIFPSESLQLRQRL---KLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNH 3783
+C G+W T C+ R + C+ + C+ L G Y VCP+G G + E + A F DS+ + + + +S+ + L+ SDG+IFY+ TD D++++ L N +E+R DLG G ++S+ L + ++I +ER R + I+ G +P ++L+ +G A P + L+ +T+GF G + KI LM+ P + +N++ S + + + +PC NGG C Y C C PGY G C + + F +F+ + + + + L K E++ A +GLL + G +R+ +I++ + +++T L S +W H+ +A + +L + +
Sbjct: 1405 KCSKGFWG------TLCADVRDPCAASKCHATS---QCQVLPEGGYKCVCPMG----REGRHCEKVAERR-GAYMPTFNGDSYLELKGLHLYGHDLRQKVSMMVVLMANDSDGLIFYNGQKTDGKGDFISLGLNNGILEFRYDLGKGPAIIRSKAPLPLKVWNTINLERSLRKGEIRINDQQPARGESPAVHSDLNLKESLFVGGA--PDYSKLARAAGLTEGFKGTVQ----------KIMLMSD---------------PVLKEENAL-----------SSSNVAMFQGHPCSQEPCQNGGRCNPML-ATYECSCFPGYVGDNCSVAIHEKSAGETEAVAFDGRTFIEYHNGVTKSEKALLVNKFELSIRTEATHGLLLWSGKGVERS-DYIALAIVDGRVQMTYDLGSRPVVLRSSVRVNTNRWIHIKASRALRDGFLQVGN 1795 HSP 5 Score: 70.0922 bits (170), Expect = 2.229e-11 Identity = 58/251 (23.11%), Postives = 107/251 (42.63%), Query Frame = 1
Query: 3340 KIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSH-IWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQ---NSDIILNNITQWQGPPCGPLQSPC 4080
K + C +H PCL+GG C+ YSC+C G G CE+V+ + I F S++ FP+ ++ +++ + F NG+L + +G + FIS+TL +E+ T G L S G W+ V + + + L+++ P + LN+++ +F GGV + + + G I+ + +N+ + Q + + + + PC P +PC
Sbjct: 1160 KTQRPCDSH-PCLHGGTCEDDGS-TYSCRCPAGRGGPVCEKVMKYFIPSFGGQSYLAFPT--MKAYHTVRIAMAFRASEMNGVLLY--NGQRGSKDFISLTLVNGKVELRFNTGSGTGTLTSKVKVSQGHWHQVEVTRNRRNGVLSVDGEPDVQGESPHGTDGLNLDT--------------ELFIGGVTEDLKQDVTARTGVSAGLVGCIRMLDVNNRMLNLQEDGGDSLFGSGVGECGNNPCEP--NPC 1388 HSP 6 Score: 55.8398 bits (133), Expect = 4.504e-7 Identity = 49/208 (23.56%), Postives = 84/208 (40.38%), Query Frame = 1
Query: 2584 IETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSG--NAENYLSIN---ISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDA-GFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGE 3189
+ T +C+C P +G CSV +I+E E Y +G +E L +N +S+ ++ G++ + SDY+ + + + V+ DLGS + L+S V++ R I R RD L + + G +P T LD G ++ G + L + GFVGC+ + + E
Sbjct: 1661 LATYECSCFPGYVGDNCSV---------------AIHEKSAGETEAVAFDGRTFIEYHNGVTKSEKALLVNKFELSIRTEATHGLLLWSGKGVERSDYIALAIVDGRVQMTYDLGSRPVVLRSSVRVNTNRWIHIKASRALRDGFLQVGNEAAVTGSSPLAATQLDTDGALWLGGLEELAVARRLPKA--YSTGFVGCIKDVVVDGVE 1851
BLAST of Agrin, putative vs. Ensembl Medaka
Match: EGFLAM (EGF like, fibronectin type III and laminin G domains [Source:NCBI gene;Acc:101173710]) HSP 1 Score: 123.25 bits (308), Expect = 1.043e-27 Identity = 144/660 (21.82%), Postives = 261/660 (39.55%), Query Frame = 1
Query: 2764 NAENYLSINISLVPYSSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEE---VVSHIWKFKDNSFVI--FPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFR--KYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVL 4716
N+ I + S DG++ Y + + D+ ++ L + YR + G+GA + S Q+ +GR H++ V R L +D G + T + +G + P L GF GC+ SL I VD+ P + S + + D C NGG+C + + Y C C G++G+ CEE + S ++ S+ + +P ++ E+TF P +G+L + D + F+++ + ++E G + S E + W+ + + + AK L ++ Q + + F +N + P++ GGV + + LKP F G IQK+++ND I N+ PC + SPC+ G C P+ + C C ++ +H A T +++G+S Y + S ++ N+ ++F++ + DG+L+ S + + +++G I + NLG +GV I N SD KWH V+ +R+ ++ L +DD + L +G +++GG K+ +Y GC+S + Y+L L
Sbjct: 351 NSYQTFQITLDFKANSEDGLLLYCGENEHGRGDFASLALIRGKIHYRFNCGTGAAQIVSESQIVVGRWHTVTVFRDGMSGWLRLDNDTPISGRSQGQYTKITFRSQLYLGGS--PSTYWLVRATGTNRGFEGCVQSLTIN--------------------------------NKVVDLRPWPLGKALSGADIGECSDSVCNQVSCANGGVCFANRADGYICLCPLGFRGLLCEESFLLSSPLFNETVFSYAVIPWPQRPQNYLSFMEFEVTFWPSTPDGVLLYSDDAASGD--FLAINIVDKYVEFRFDCGSGEAVIRSDEQISLDSWHELRVTRTAKSGILQVDS--QRAVEGISEGAFTQINC------------SSPLYIGGV-PHYDKTKRTARVLKP-FTGIIQKLILNDRTISITAGSAGGVNVIN-SAHPC--VDSPCAN----GGTCRPKWDGYECDCPLGYDG-------RHCQKAVTEAIEIPQFIGRSYLTYDNRDILKRVSGSRTNLFMRFRSTAKDGLLLWRGDSPLRSNSDFLSMGLQDGNIVFSYNLG--SGVASIVVNGTFSDGKWHKVKAVRDGQSGKLTVDDYGAKTGR-SPGKMRQLNINGPLYVGGMKEIALHTNRQYFGGLVGCVSHFTL-STDYHLAL 940 HSP 2 Score: 90.5077 bits (223), Expect = 1.006e-17 Identity = 103/436 (23.62%), Postives = 177/436 (40.60%), Query Frame = 1
Query: 2629 YCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSI---NISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS---HIWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNS-LVSRKLTQFRIKNQPIFFGGV 3903
Y +CP+G+ C+E+ + + + E F S+A W +NYLS ++ P + DG++ Y SD S D+L I + ++YVE+R D GSG ++S Q+++ H + V R + L +D + G++ T ++ IG P + T V F G + L ++N +T I S V+VI S PC NGG C+ + Y C C GY G C++ V+ I +F S++ + + + R R L + F A +GLL + D R N F+S+ L ++ + GV ++ + GKW+ V ++ + L ++ + LN+N L + + + +FGG+
Sbjct: 535 YICLCPLGFR--GLLCEESFLLSSPL-FNETVF---SYAVIPWPQRPQNYLSFMEFEVTFWPSTPDGVLLY-SDDAASGDFLAINIVDKYVEFRFDCGSGEAVIRSDEQISLDSWHELRVTRTAKSGILQVDSQRAVEGISEGAFTQINCSSPLYIGGV--PHYDKTKRTARVLKPFTGIIQKL-----------ILNDRTISITAGS-----------AGGVNVINSAHPCVDS---------------PCANGGTCRPKWDG-YECDCPLGYDGRHCQKAVTEAIEIPQFIGRSYLTYDNRDILKRVSGSRTNLFMRFRSTAKDGLLLWRGDSPLRSNSDFLSMGLQDGNIVFSYNLGSGVASIVVNGTFSDGKWHKVKAVRDGQSGKLTVDDYGAKTGRSPGKMRQLNINGPLYVGGMKEIALHTNRQYFGGL 923
BLAST of Agrin, putative vs. Ensembl Medaka
Match: EGFLAM (EGF like, fibronectin type III and laminin G domains [Source:NCBI gene;Acc:101173710]) HSP 1 Score: 122.479 bits (306), Expect = 1.709e-27 Identity = 144/660 (21.82%), Postives = 261/660 (39.55%), Query Frame = 1
Query: 2764 NAENYLSINISLVPYSSDGIIFYHSDTDRS-SDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEE---VVSHIWKFKDNSFVI--FPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFR--KYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXV-IAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVL 4716
N+ I + S DG++ Y + + D+ ++ L + YR + G+GA + S Q+ +GR H++ V R L +D G + T + +G + P L GF GC+ SL I VD+ P + S + + D C NGG+C + + Y C C G++G+ CEE + S ++ S+ + +P ++ E+TF P +G+L + D + F+++ + ++E G + S E + W+ + + + AK L ++ Q + + F +N + P++ GGV + + LKP F G IQK+++ND I N+ PC + SPC+ G C P+ + C C ++ +H A T +++G+S Y + S ++ N+ ++F++ + DG+L+ S + + +++G I + NLG +GV I N SD KWH V+ +R+ ++ L +DD + L +G +++GG K+ +Y GC+S + Y+L L
Sbjct: 451 NSYQTFQITLDFKANSEDGLLLYCGENEHGRGDFASLALIRGKIHYRFNCGTGAAQIVSESQIVVGRWHTVTVFRDGMSGWLRLDNDTPISGRSQGQYTKITFRSQLYLGGS--PSTYWLVRATGTNRGFEGCVQSLTIN--------------------------------NKVVDLRPWPLGKALSGADIGECSDSVCNQVSCANGGVCFANRADGYICLCPLGFRGLLCEESFLLSSPLFNETVFSYAVIPWPQRPQNYLSFMEFEVTFWPSTPDGVLLYSDDAASGD--FLAINIVDKYVEFRFDCGSGEAVIRSDEQISLDSWHELRVTRTAKSGILQVDS--QRAVEGISEGAFTQINC------------SSPLYIGGV-PHYDKTKRTARVLKP-FTGIIQKLILNDRTISITAGSAGGVNVIN-SAHPC--VDSPCAN----GGTCRPKWDGYECDCPLGYDG-------RHCQKAVTEAIEIPQFIGRSYLTYDNRDILKRVSGSRTNLFMRFRSTAKDGLLLWRGDSPLRSNSDFLSMGLQDGNIVFSYNLG--SGVASIVVNGTFSDGKWHKVKAVRDGQSGKLTVDDYGAKTGR-SPGKMRQLNINGPLYVGGMKEIALHTNRQYFGGLVGCVSHFTL-STDYHLAL 1040 HSP 2 Score: 90.5077 bits (223), Expect = 1.128e-17 Identity = 103/436 (23.62%), Postives = 177/436 (40.60%), Query Frame = 1
Query: 2629 YCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSI---NISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVS---HIWKFKDNSFVIFPSESLQLR---QRLKLEITFLPLANNGLLSFISDGHDR-NPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNS-LVSRKLTQFRIKNQPIFFGGV 3903
Y +CP+G+ C+E+ + + + E F S+A W +NYLS ++ P + DG++ Y SD S D+L I + ++YVE+R D GSG ++S Q+++ H + V R + L +D + G++ T ++ IG P + T V F G + L ++N +T I S V+VI S PC NGG C+ + Y C C GY G C++ V+ I +F S++ + + + R R L + F A +GLL + D R N F+S+ L ++ + GV ++ + GKW+ V ++ + L ++ + LN+N L + + + +FGG+
Sbjct: 635 YICLCPLGFR--GLLCEESFLLSSPL-FNETVF---SYAVIPWPQRPQNYLSFMEFEVTFWPSTPDGVLLY-SDDAASGDFLAINIVDKYVEFRFDCGSGEAVIRSDEQISLDSWHELRVTRTAKSGILQVDSQRAVEGISEGAFTQINCSSPLYIGGV--PHYDKTKRTARVLKPFTGIIQKL-----------ILNDRTISITAGS-----------AGGVNVINSAHPCVDS---------------PCANGGTCR-PKWDGYECDCPLGYDGRHCQKAVTEAIEIPQFIGRSYLTYDNRDILKRVSGSRTNLFMRFRSTAKDGLLLWRGDSPLRSNSDFLSMGLQDGNIVFSYNLGSGVASIVVNGTFSDGKWHKVKAVRDGQSGKLTVDDYGAKTGRSPGKMRQLNINGPLYVGGMKEIALHTNRQYFGGL 1023
BLAST of Agrin, putative vs. Planmine SMEST
Match: SMESG000028865.1 (SMESG000028865.1) HSP 1 Score: 3066.56 bits (7949), Expect = 0.000e+0 Identity = 1541/1566 (98.40%), Postives = 1541/1566 (98.40%), Query Frame = 1
Query: 103 MMXXXXXXXXGFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEY----------------RVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
MMLLVLLLFLGFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEY RVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDSNTFNSFSNVVSLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC
Sbjct: 1 MMLLVLLLFLGFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK---------RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYSDKIIHIIIVHYYTYFRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDSNTFNSFSNVVSLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 1557
BLAST of Agrin, putative vs. Planmine SMEST
Match: SMESG000028865.1 (SMESG000028865.1) HSP 1 Score: 3065.02 bits (7945), Expect = 0.000e+0 Identity = 1531/1556 (98.39%), Postives = 1531/1556 (98.39%), Query Frame = 1
Query: 133 GFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEY----------------RVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
GFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEY RVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDSNTFNSFSNVVSLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC
Sbjct: 10 GFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK---------RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYSDKIIHIIIVHYYTYFRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDSNTFNSFSNVVSLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 1556
BLAST of Agrin, putative vs. Planmine SMEST
Match: SMESG000028865.1 (SMESG000028865.1) HSP 1 Score: 3027.27 bits (7847), Expect = 0.000e+0 Identity = 1526/1566 (97.45%), Postives = 1526/1566 (97.45%), Query Frame = 1
Query: 103 MMXXXXXXXXGFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEY----------------RVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
MMLLVLLLFLGFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEY RVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRL LTQFRIKNQPIFFGGVDSNTFNSFSNVVSLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC
Sbjct: 1 MMLLVLLLFLGFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVIVSVIRILKGFDLINEVKKISHENDYHLILVRKIKYIINCKISIKSGEDFIMYLKKTEQNNFFELNYAKEFGNGLFEKRISVFLNGATSKQVQNDDPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQKEISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDICKFVKCEFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK---------RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYSDKIIHIIIVHYYTYFRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRL---------------LTQFRIKNQPIFFGGVDSNTFNSFSNVVSLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 1542
BLAST of Agrin, putative vs. Planmine SMEST
Match: SMESG000028859.1 (SMESG000028859.1) HSP 1 Score: 2135.92 bits (5533), Expect = 0.000e+0 Identity = 1085/1207 (89.89%), Postives = 1117/1207 (92.54%), Query Frame = 1
Query: 1138 VCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFC--ENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
VCG++ Y + CHL+ E+C I + + Y G C CS + C + C +N +C C CK+ +D +CG+ G YDN C L C+ ++++N C ENNPC+GVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQ K+CPSYQICKSDLVQ KCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCIS NYIADEKNK+SCLCNSIGSLN+ECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCN ETGKCNCKPLAMGKYCSVCPIGYSISNHGCK+NSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSG LKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDV DGFVGCLSSLKITAGEETKIY MNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSS++MYSCQCLPGYQGI CEEVVSHIWKFKDNSFVIFPSES+QLRQRLKLEITFLPLANNGLLSFISDGHDRN AFISVTLNKN LEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRL LTQFRIKNQP+FFGGVDSNTFNSFSNVVSLKPNF GAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNP+NTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWH+VQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTL TDGWMWLGGAK PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPIL+C
Sbjct: 74 VCGTNLKQYATVCHLRREACTLMIDIGIKYHGLCNP------CSLVNCSHNM-CSLDANRKPKCDCPST-CKDDPKDLLCGSSGKTYDNLCLLNLDKCQTGQHIYIVNKGPCQFENNPCNGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQEKECPSYQICKSDLVQTKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISQNYIADEKNKKSCLCNSIGSLNNECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNTETGKCNCKPLAMGKYCSVCPIGYSISNHGCKKNSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGVNLLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVIDGFVGCLSSLKITAGEETKIYRMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSKKMYSCQCLPGYQGIICEEVVSHIWKFKDNSFVIFPSESVQLRQRLKLEITFLPLANNGLLSFISDGHDRNTAFISVTLNKNQLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRL---------------LTQFRIKNQPLFFGGVDSNTFNSFSNVVSLKPNFNGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPSNTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHSVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLETDGWMWLGGAKIPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILKC 1257 HSP 2 Score: 138.272 bits (347), Expect = 2.053e-32 Identity = 154/519 (29.67%), Postives = 233/519 (44.89%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQK--EISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDI----CKFVKC-EFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKC----QTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDC-KNTWRDPVCGNDGIIYDNKCFLMKMSCE-KQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICN------EEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETN-SIALKIANIG------TCDKCKNKKCSFYSICQMNRFLEP---QCICPT 2007
+PC +CK+ A C N +A C C + G SVCG++ Y + C ++R+ C L I + + +C + S N C H C A N P C CP+ D +CG+ TY N+C + C+ + I G C C VKC + C + +G T C+CP E C K +Q PVCG+D VTY+S C+L +C K L ++Y G C + K Q C + Q + + Q C+ C ++ + VCG DG Y ++C + +CE + + + N C+ C KC Y C+++ + C CP + C + +PVCG+D TYE+ C L +C + + + G C +E RC E DCP ++C++ +C C T S + VCGTDG Y++EC ++ AC N +I + + IG D+ K C SI +N +P QCIC T
Sbjct: 32 NPCLNHSCKYQAWCIP-NSNFTSANCICYTSCFDVGDSTDSESVCGTNLKQYATVCHLRREACTLMIDIGIKYHGLC-------NPCSLVN---CSHNMCSLDA----NRKPKCDCPSTCKDDPKDLLCGSSGKTYDNLCLLNLDKCQTGQHIYIVNKGPCQFENNPCNGVKCPDPYQYCRIDNNGKST-CECPK--EPCPKIVQ-PVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQEKECPSYQI--------CKSDLVQTKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDK--CKNKKC-SFYSICQMNRFLEPQCICPTD-CVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPE---DCPLGKMCRN----GQCVC-TDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISQNYIADEKNKKSCLCNSIGSLNNECDPSTTQCICKT 511
BLAST of Agrin, putative vs. Planmine SMEST
Match: SMESG000028859.1 (SMESG000028859.1) HSP 1 Score: 2110.11 bits (5466), Expect = 0.000e+0 Identity = 1076/1207 (89.15%), Postives = 1108/1207 (91.80%), Query Frame = 1
Query: 1138 VCGSDDVTYQSECHLKIESCKKKISLTVMYKGKCQTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDCKNTWRDPVCGNDGIIYDNKCFLMKMSCEKQMEMHVINSNFC--ENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCKLQFIKPDEERCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISHNYIADEKNKRSCLCNSIGSLNHECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNIETGKCNCKPLAMGKYCSVCPIGYSISNHGCKENSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGAIFLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVTDGFVGCLSSLKITAGEETKIYLMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSEEMYSCQCLPGYQGITCEEVVSHIWKFKDNSFVIFPSESLQLRQRLKLEITFLPLANNGLLSFISDGHDRNPAFISVTLNKNHLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRLIPKGFLNVNSLVSRKLTQFRIKNQPIFFGGVDXXXXXXXXXXXXLKPNFYGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPANTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMXXXXXXXXXXXXXXXVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHTVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLATDGWMWLGGAKKPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILRC 4752
VCG++ Y + CHL+ E+C I + + Y G C CS + C + C +N +C C CK+ +D +CG+ G YDN C L C+ ++++N C ENNPC+GVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQ K+CPSYQICKSDLVQ KCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCIS NYIADEKNK+SCLCNSIGSLN+ECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCN ETGKCNCKPLAMGKYCSVCPIGYSISNHGCK+NSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSG LKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDV DGFVGCLSSLKITAGEETKIY MNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSS++MYSCQCLPGYQGI CEEVVSHIWKFKDNSFVIFPSES+QLRQRLKLEITFLPLANNGLLSFISDGHDRN AFISVTLNKN LEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRL LTQFRIKNQP+FFGGVDSNTFNSFSNVVSLKPNF GAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNP+NTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWH+VQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTL TDGWMWLGGAK PVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPIL+C
Sbjct: 74 VCGTNLKQYATVCHLRREACTLMIDIGIKYHGLCNP------CSLVNCSHNM-CSLDANRKPKCDCPST-CKDDPKDLLCGSSGKTYDNLCLLNLDKCQTGQHIYIVNKGPCQFENNPCNGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQEKECPSYQICKSDLVQTKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDKCKNKKCSFYSICQMNRFLEPQCICPTDCVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK---------RCPEDCPLGKMCRNGQCVCTDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISQNYIADEKNKKSCLCNSIGSLNNECDPSTTQCICKTGITGQYCDRCIDGYWNFTENGCTACSCNRFGSESIVCNTETGKCNCKPLAMGKYCSVCPIGYSISNHGCKKNSIYENKVDAVELGFLKDSFAKSYWSGNAENYLSINISLVPYSSDGIIFYHSDTDRSSDYLTIVLKNRYVEYRVDLGSGVNLLKSRVQLTIGRSHSILVERLNRDSRLVIDGTDSQYGMNPPFLTNLDAGFIFTIGQATGPIINTLSHTGDVIDGFVGCLSSLKITAGEETKIYRMNQQTSWIIESSNVKPNCPHIDFKNSVDVIEDPDKEEQSEKENLKIKDYCQTHKPCLNGGLCQTSSKKMYSCQCLPGYQGIICEEVVSHIWKFKDNSFVIFPSESVQLRQRLKLEITFLPLANNGLLSFISDGHDRNTAFISVTLNKNQLEVTCRTPIGVTRLISMESALIGKWNHVVILKAAKYIYLNLNHSPQPIKKRL---------------LTQFRIKNQPLFFGGVDSNTFNSFSNVVSLKPNFNGAIQKILINDENIRFQNSDIILNNITQWQGPPCGPLQSPCSKTDPEKGFCVPEMNETICTCSPKHENLCIRNSMQHDNPSNTNFFRKYLGKSASKYAAVKQKRDKSSTKNNIRIKFKTASNDGMLILLKKIYSIKKSFLVIAIRNGVIEVAINLGGKNGVLRITGNKNISDNKWHSVQVIRNNRNVVLLIDDRMFRAQMLDNNDYNTLETDGWMWLGGAKIPVEGFPWEYNTKFKGCISELYVDEISYNLVLDARKNIGPILKC 1248 HSP 2 Score: 137.502 bits (345), Expect = 3.726e-32 Identity = 153/513 (29.82%), Postives = 231/513 (45.03%), Query Frame = 1
Query: 538 DPCSKLNCKFGAQCQKWPGNPPTAVCHCSNTQCQAGQK--EISVCGSDGIFYDSHCLMKRQECFLQKSIQVSPNHICGRKEAQQDGISKSNEQFCQHQSCPPYATCVTNPYPYCKCPNDKCDSSSSQICGTDEITYTNICEMKKVSCKANRFIEQQYVGRCDI----CKFVKC-EFNGICEMMEDGLGTICKCPNPDELCTKNIQVPVCGSDDVTYQSECHLKIESCKKKISLTVMYKGKC----QTKIQSDICSNIQCHYGAKCIKTSNTVAQCSCDYLDC-KNTWRDPVCGNDGIIYDNKCFLMKMSCE-KQMEMHVINSNFCENNPCSGVKCPDPYQYCRIDNNGKSTCECPKEPCPKIVQPVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQGKDCPSYQICKSDLVQAKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETN-SIALKIANIG------TCDKCKNKKCSFYSICQMNRFLEP---QCICPT 2007
+PC +CK+ A C N +A C C + G SVCG++ Y + C ++R+ C L I + + +C + S N C H C A N P C CP+ D +CG+ TY N+C + C+ + I G C C VKC + C + +G T C+CP E C K +Q PVCG+D VTY+S C+L +C K L ++Y G C + K Q C + Q + + Q C+ C ++ + VCG DG Y ++C + +CE + + + N C+ C KC Y C+++ + C CP + C + +PVCG+D TYE+ C L +C + + + G C RC E DCP ++C++ +C C T S + VCGTDG Y++EC ++ AC N +I + + IG D+ K C SI +N +P QCIC T
Sbjct: 32 NPCLNHSCKYQAWCIP-NSNFTSANCICYTSCFDVGDSTDSESVCGTNLKQYATVCHLRREACTLMIDIGIKYHGLC-------NPCSLVN---CSHNMCSLDA----NRKPKCDCPSTCKDDPKDLLCGSSGKTYDNLCLLNLDKCQTGQHIYIVNKGPCQFENNPCNGVKCPDPYQYCRIDNNGKST-CECPK--EPCPKIVQ-PVCGNDKVTYESICNLTRTACLTKKPLWIIYNGICNEEPRCKEQEKECPSYQI--------CKSDLVQTKCECPTCPQSGLGESVCGTDGKTYKSECHMRMHACETNSIALKIANIGTCDK--CKNKKC-SFYSICQMNRFLEPQCICPTD-CVYVRRPVCGSDDRTYENECFLKVKSCAENRLITAVSEGPCK---RCPE---DCPLGKMCRN----GQCVC-TDKCSKVKLRVCGTDGNYYENECELKRKACIDNKTIRVAPSVIGCISQNYIADEKNKKSCLCNSIGSLNNECDPSTTQCICKT 502 The following BLAST results are available for this feature:
BLAST of Agrin, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Agrin, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Agrin, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Agrin, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Agrin, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Agrin, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021589 ID=SMED30021589|Name=Agrin, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=4897bpback to top protein sequence of SMED30021589-orf-1 >SMED30021589-orf-1 ID=SMED30021589-orf-1|Name=SMED30021589-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1551bp MMLLVLLLFLGFQRNVLSTDCDLSNQFNQKIESSDLIIVGRVLNLANSEVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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