Reverse transcriptase

Overview
NameReverse transcriptase
Smed IDSMED30021552
Length (bp)3075
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021552

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30021552SMESG000050280.1 dd_Smed_v4_7330_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021552SMESG000050280.1 dd_Smed_v4_7330_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: K7JS44 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.413e-12
Identity = 49/181 (27.07%), Postives = 91/181 (50.28%), Query Frame = 2 
Query: 2366 KNNLDTKWRLCHLDNETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIP-TKLKPDMTIRSKRNKEIHVLDLKVPYDS-VKSFDKAREANYEKYKDLAI*IGEAFKERATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHIS 2902
            +  +D + R C  D ET  HV +HC     AR+ +H N+   +V    +    I V ++   ++ P   L+PD+ +R   N+ + ++D+ VP+++ +++FD  REA   KY  LA  + ++     TV A V+G L   D +N   L+ + +++     + ++     +     IY EH+S
Sbjct:  173 QRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDSGYT-VTVEAFVVGALGAWDPRNERVLSLLSISRYYAILMRRLMVSDTIRWSRDIYVEHVS 352
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: K7JWY8 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.439e-12
Identity = 49/181 (27.07%), Postives = 91/181 (50.28%), Query Frame = 2 
Query: 2366 KNNLDTKWRLCHLDNETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIP-TKLKPDMTIRSKRNKEIHVLDLKVPYDS-VKSFDKAREANYEKYKDLAI*IGEAFKERATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHIS 2902
            +  +D + R C  D ET  HV +HC     AR+ +H N+   +V    +    I V ++   ++ P   L+PD+ +R   N+ + ++D+ VP+++ +++FD  REA   KY  LA  + ++     TV A V+G L   D +N   L+ + +++     + ++     +     IY EH+S
Sbjct:  173 QRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDSGYT-VTVEAFVVGALGAWDPRNERVLSLLSISRYYAILMRRLMVSDTIRWSRDIYVEHVS 352
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: K7JS43 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.465e-12
Identity = 49/181 (27.07%), Postives = 91/181 (50.28%), Query Frame = 2 
Query: 2366 KNNLDTKWRLCHLDNETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIP-TKLKPDMTIRSKRNKEIHVLDLKVPYDS-VKSFDKAREANYEKYKDLAI*IGEAFKERATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHIS 2902
            +  +D + R C  D ET  HV +HC     AR+ +H N+   +V    +    I V ++   ++ P   L+PD+ +R   N+ + ++D+ VP+++ +++FD  REA   KY  LA  + ++     TV A V+G L   D +N   L+ + +++     + ++     +     IY EH+S
Sbjct:  173 QRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDSGYT-VTVEAFVVGALGAWDPRNERVLSLLSISRYYAILMRRLMVSDTIRWSRDIYVEHVS 352
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: B4P1Z7 (Uncharacterized protein OS=Drosophila yakuba OX=7245 GN=Dyak\GE12380 PE=4 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 1.576e-9
Identity = 49/188 (26.06%), Postives = 90/188 (47.87%), Query Frame = 2 
Query: 2378 DTKWRLCHLDNETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIG---FEIDVEKSPKYLKIPTKLKPDMTIRSKRNKEIHVLDLKVPYD-SVKSFDKAREANYEKYKDLAI*IGEAFKERATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHISYTKSAIVLP 2929
            D + R+C    E+  HV  HC    NA + +H  V+  +     ++G       VE   + +     L+PD+  R +R+++I ++D+ VP++   ++FD AR    EKY+ LA  +  A   +  + A ++G L + D KN   L  +G+++     + ++     +     IY EH+S  +    LP
Sbjct:  176 DKRCRVCGYQLESVPHVLCHCMHHSNAMQQRHNAVMDRLAKAGSRLGTPRVNCRVEGVAEDM---AALRPDLVWRDERSRKIVIVDVTVPFENGAEAFDNARGEKEEKYRPLAEAL-RAMGYQVKLEAFIVGALGSWDPKNERVLKTLGVSRFYAGLMRRLMVADTIRWSRDIYVEHVSGIRQ-FTLP 358
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: E2BTP9 (Uncharacterized protein (Fragment) OS=Harpegnathos saltator OX=610380 GN=EAI_12932 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.718e-9
Identity = 46/168 (27.38%), Postives = 80/168 (47.62%), Query Frame = 2 
Query: 2411 ETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIPTKLKPDMTIRSKRNKEIHVLDLKVPYDSVK-SFDKAREANYEKYKDLAI*IGEAFKE---RATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHIS 2902
            ET  H+F HC     A + +H+++L+ +       G     ++         +L+PD+ I+ + +K +   DL VP+++   +F++ARE  + KY  LA    EAF     R  V+A V+G L++ D  N P L  + +       + ++     +     IY EHIS
Sbjct:    1 ETLPHMFCHCRLHSAAIQWRHDSILLRLSRACRLPGHVTVNQRVEGLYGELGELRPDLVIKHEPSKSVATCDLMVPFENRPIAFEEARERKFAKYAPLA----EAFSRLGYRVIVTAFVVGALDSWDPCNEPVLGLLRIGSTYASMMRRLIVSDTIRWFRDIYVEHIS 164
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000050280.1 (SMESG000050280.1)

HSP 1 Score: 928.702 bits (2399), Expect = 0.000e+0
Identity = 471/472 (99.79%), Postives = 471/472 (99.79%), Query Frame = 2 
Query:  137 MKSMISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTXXXXXXXXXXXXXXCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 1552
            MK MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ
Sbjct:    1 MKFMISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 472
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000050280.1 (SMESG000050280.1)

HSP 1 Score: 925.62 bits (2391), Expect = 0.000e+0
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 2 
Query:  146 MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTXXXXXXXXXXXXXXCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 1552
            MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ
Sbjct:    1 MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 469
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 444.121 bits (1141), Expect = 1.390e-132
Identity = 241/330 (73.03%), Postives = 280/330 (84.85%), Query Frame = 2 
Query: 1925 SEARW*KNFEAGLKGMNGEVSKQFPNTSXXXXXXXXXXXXXXXXCICFKLNFVDDTFSLEMSYKKRISYINIQNLKTLSKELHDFVGLHYTEQWCQMHVRGHIATTIGDSITAKYLVASDTLNDAQYTFXXXXXXXXXXXXXXXXXMKNNLDTKWRLCHLDNETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIPTKLKPDMTIRSKRNKEIHVLDLKVPYDSVKSFDKAREANYEKYKDLAI*IGEAFKERATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHISYTKS 2914
            +++R   NFEAGL  MN E +K F  +SFF KFQK+A +LKRKFCI   L F++D F+LE++YKKRISYIN QNL TLSKELH+F+GLHY EQWC+M V+GHIA +IGDSITAKYL+ASDTLNDAQY FL+RARNN+L LNY  YR+K N++TK RLCHLD ETQ HVFNHC AKPNARR+KHENVL+ IV+FLEKIGFEIDVEKSPKY+ IPTKLKPDM IRSKRN++IHVLDLKVPYDS++ F+KARE NY KYKDLA+ IG+AF + AT+SA+VIGCL T DKKNN  L+KIGLTK EI  LAKIAC  AV ACYHIYREHIS+TKS
Sbjct: 2182 AQSRGNGNFEAGLSWMNCEFNKIFLKSSFFVKFQKSAQSLKRKFCIYVNLKFIEDNFTLEITYKKRISYINNQNLNTLSKELHNFIGLHYAEQWCKMRVQGHIAASIGDSITAKYLIASDTLNDAQYYFLVRARNNLLKLNYGAYRLKYNINTKCRLCHLDEETQAHVFNHCQAKPNARRVKHENVLLGIVSFLEKIGFEIDVEKSPKYVSIPTKLKPDMVIRSKRNRDIHVLDLKVPYDSIEGFEKAREDNYVKYKDLAMQIGKAFNQTATISAVVIGCLGTWDKKNNATLSKIGLTKTEIIALAKIACSDAVIACYHIYREHISFTKS 2511
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 444.121 bits (1141), Expect = 1.432e-132
Identity = 241/330 (73.03%), Postives = 280/330 (84.85%), Query Frame = 2 
Query: 1925 SEARW*KNFEAGLKGMNGEVSKQFPNTSXXXXXXXXXXXXXXXXCICFKLNFVDDTFSLEMSYKKRISYINIQNLKTLSKELHDFVGLHYTEQWCQMHVRGHIATTIGDSITAKYLVASDTLNDAQYTFXXXXXXXXXXXXXXXXXMKNNLDTKWRLCHLDNETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIPTKLKPDMTIRSKRNKEIHVLDLKVPYDSVKSFDKAREANYEKYKDLAI*IGEAFKERATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHISYTKS 2914
            +++R   NFEAGL  MN E +K F  +SFF KFQK+A +LKRKFCI   L F++D F+LE++YKKRISYIN QNL TLSKELH+F+GLHY EQWC+M V+GHIA +IGDSITAKYL+ASDTLNDAQY FL+RARNN+L LNY  YR+K N++TK RLCHLD ETQ HVFNHC AKPNARR+KHENVL+ IV+FLEKIGFEIDVEKSPKY+ IPTKLKPDM IRSKRN++IHVLDLKVPYDS++ F+KARE NY KYKDLA+ IG+AF + AT+SA+VIGCL T DKKNN  L+KIGLTK EI  LAKIAC  AV ACYHIYREHIS+TKS
Sbjct: 2203 AQSRGNGNFEAGLSWMNCEFNKIFLKSSFFVKFQKSAQSLKRKFCIYVNLKFIEDNFTLEITYKKRISYINNQNLNTLSKELHNFIGLHYAEQWCKMRVQGHIAASIGDSITAKYLIASDTLNDAQYYFLVRARNNLLKLNYGAYRLKYNINTKCRLCHLDEETQAHVFNHCQAKPNARRVKHENVLLGIVSFLEKIGFEIDVEKSPKYVSIPTKLKPDMVIRSKRNRDIHVLDLKVPYDSIEGFEKAREDNYVKYKDLAMQIGKAFNQTATISAVVIGCLGTWDKKNNATLSKIGLTKTEIIALAKIACSDAVIACYHIYREHISFTKS 2532
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 444.121 bits (1141), Expect = 1.465e-132
Identity = 241/330 (73.03%), Postives = 280/330 (84.85%), Query Frame = 2 
Query: 1925 SEARW*KNFEAGLKGMNGEVSKQFPNTSXXXXXXXXXXXXXXXXCICFKLNFVDDTFSLEMSYKKRISYINIQNLKTLSKELHDFVGLHYTEQWCQMHVRGHIATTIGDSITAKYLVASDTLNDAQYTFXXXXXXXXXXXXXXXXXMKNNLDTKWRLCHLDNETQTHVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIPTKLKPDMTIRSKRNKEIHVLDLKVPYDSVKSFDKAREANYEKYKDLAI*IGEAFKERATVSAIVIGCLETCDKKNNPALAKIGLTKPEIKTLAKIACPKAVTACYHIYREHISYTKS 2914
            +++R   NFEAGL  MN E +K F  +SFF KFQK+A +LKRKFCI   L F++D F+LE++YKKRISYIN QNL TLSKELH+F+GLHY EQWC+M V+GHIA +IGDSITAKYL+ASDTLNDAQY FL+RARNN+L LNY  YR+K N++TK RLCHLD ETQ HVFNHC AKPNARR+KHENVL+ IV+FLEKIGFEIDVEKSPKY+ IPTKLKPDM IRSKRN++IHVLDLKVPYDS++ F+KARE NY KYKDLA+ IG+AF + AT+SA+VIGCL T DKKNN  L+KIGLTK EI  LAKIAC  AV ACYHIYREHIS+TKS
Sbjct: 2211 AQSRGNGNFEAGLSWMNCEFNKIFLKSSFFVKFQKSAQSLKRKFCIYVNLKFIEDNFTLEITYKKRISYINNQNLNTLSKELHNFIGLHYAEQWCKMRVQGHIAASIGDSITAKYLIASDTLNDAQYYFLVRARNNLLKLNYGAYRLKYNINTKCRLCHLDEETQAHVFNHCQAKPNARRVKHENVLLGIVSFLEKIGFEIDVEKSPKYVSIPTKLKPDMVIRSKRNRDIHVLDLKVPYDSIEGFEKAREDNYVKYKDLAMQIGKAFNQTATISAVVIGCLGTWDKKNNATLSKIGLTKTEIIALAKIACSDAVIACYHIYREHISFTKS 2540
The following BLAST results are available for this feature:
BLAST of Reverse transcriptase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K7JS441.413e-1227.07C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JWY81.439e-1227.07C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JS431.465e-1227.07C2H2-type domain-containing protein OS=Nasonia vit... [more]
B4P1Z71.576e-926.06Uncharacterized protein OS=Drosophila yakuba OX=72... [more]
E2BTP92.718e-927.38Uncharacterized protein (Fragment) OS=Harpegnathos... [more]
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BLAST of Reverse transcriptase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000050280.10.000e+099.79SMESG000050280.1[more]
SMESG000050280.10.000e+0100.00SMESG000050280.1[more]
SMESG000002829.11.390e-13273.03SMESG000002829.1[more]
SMESG000002829.11.432e-13273.03SMESG000002829.1[more]
SMESG000002829.11.465e-13273.03SMESG000002829.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021552 ID=SMED30021552|Name=Reverse transcriptase|organism=Schmidtea mediterranea sexual|type=transcript|length=3075bp
TTTCAAACAAATCGTCAGATCTATGTTTAACAATAATTTAAACAAATCAT
AAAATGACATTAATATATCTTATTTGTTCGATGTATTAATTTAATTATAA
ATAAAAGTTTATTTAAACAAAAGTTAATATTTTATTATGAAGTCTATGAT
CTCATTTTTGTTAACAAATTTTCTCATTTCTTTTATCTCAGCAAACTATA
CTTACAGTGAAAGTCAGGGAGATCTTCAAAAGGAATTCAACTTTTCGCTG
TTCATTGAAACATTCAGTGGAATTACTCTCTATGCCGTCGATTCCAATAA
TAATAAATTTCTGATAGAAATGGAACTTGAAGAAAATTTGCAAATCGCGA
TAATAAAATACAACAAGTTGTTCAATCGAGTAAAATTAGAAAATTTATGC
GGGTGCCGATTTTGCTCCCATGCCAGCATGGCATTCGTTGTCCGGTTACG
TCGGTTGGAGGTGAATGCAAAATGTAAAGGAGTTGACAAATTGATAAGAT
TGGAAAATGTCAATGGTGACGGTCAATTTAACGGATACCCATTACCGAGG
AAACGTTCTAATAATACAAACAAAATATATTTCTGGATCGGAAATATTGG
TTATTACTTTGGATTGGGTAAAATTTTATATGTACCATTAAATAACCAAT
TAACGGGTTGCTTGGACAAATTTTATTTCAATAACCGATTTCTAACCTTT
GAACAGAAAGGTAAAATGATGTTGAACTCGTGCCCTAATGTGTTCAATGA
ATCGGTTTGCAATGAAACCTCTTGTATGGCTGATCAGATATCATACGGCA
TAAATGACTGCGCTCTTGCGTCCGTGAATCCAAGATATTGTAGAAAAGCT
TTGCCATTATATTCGAAATCAAAAGATTTATACGAAATCAAGATACGAGC
GAAATTCCCAATTACGATCCGATTAGAGTGGAAATCATTCTACTTGAAGA
ATTTCTGTTTAATTGACATCAAAATGGGAAAAAACAAGTTCATTACAATG
TATTTAAACAAAATGAATCAAATATCTTTTGCAATAAGTCACAACCGCTC
TATTGTTGTTGAGAGAAATTTAACGAAAATGAAAGAGCTGGACAATAATT
TGTTAATTATCATTTTTAACGTTACTCAATTCATGATTAGAGCGGCAAGT
GCGAATGAAATATACACGTTTATTGAACCGTTTAATGAACGACCAATGGA
AATGCGAATCGGAAGTGGTGGGAATATAAATATCTGTCAAACCTTTGAAG
GGGCCATTGATATTTTTTACGATCCAACTCAAATCAGCTTACGAACAGGT
CAAAACCATTTTCAACAATCAAAGTTTAATATAATTCAACTTACCATTGG
TGTTTCGGTTGTTTTTATTGGAATCTTTATTTTAGTATGTCTTTTCAGAG
GCAATCTTAAAAAATTAAATGACCATGTAACCATAAATGAGTATGAAAAT
GGCGATCACATTCTTGTAAGATGCTCTTCCATCAAAAATATATCAGACCC
GATTCGGAAAAATGACACAACGGAAGAATCTATTTACGAAACTATATCCC
AATAAATTCTTTGTCATTGGCTTAATTATTTAAAATTTTGAATGGTCAGC
TATGCGGTTAGCTTTACTTGAAATGGAGTTGATTAATGTTTTAACCTATT
CTTCAATATTTCTCCGATTAAATTATATACTATGTATATAATCTGCTATT
AACCAATGAGACATTTTTATGGTGCAAAGAAAATACACAAACGCTGAATT
AACTAATAATTGGCTGCCTATATTTTTCCTACTGAAATATATGAATTGAA
CCGGATGTTATTTGTAGTAAGTTAAAAAATTCTAAAACAAAACATGAATC
AGATTTGATATATTCCACAAACATAGAAATTAAATGATATTCCATTCGGT
TTTATTTTGAGGATTCATTTGTATTCTGAAGCACGATGGTAGAAAAACTT
TGAAGCAGGACTTAAAGGGATGAATGGCGAGGTATCCAAGCAATTTCCTA
ATACTTCTTTCTTCAAAAAATTTCAGAAAGCAGCACTTGCTCTTAAAAGA
AAATTCTGTATATGCTTTAAACTTAACTTTGTAGATGACACTTTTTCACT
TGAAATGTCCTACAAAAAACGCATCTCTTATATAAATATACAGAATTTAA
AAACTCTTTCTAAAGAACTTCACGACTTCGTGGGTCTTCATTATACTGAG
CAATGGTGTCAAATGCACGTGCGTGGACACATTGCAACTACGATTGGAGA
CAGCATTACTGCTAAGTACTTGGTTGCTAGCGACACGCTGAATGATGCGC
AATATACCTTCTTAATACGCGCTAGAAACAACATTCTAAACCTGAATTAC
AATCCTTACAGAATGAAGAATAACCTCGATACAAAATGGAGACTGTGCCA
TCTGGACAATGAAACTCAGACCCATGTGTTTAATCACTGCCCAGCCAAGC
CTAATGCTAGGAGAATCAAGCATGAAAATGTCCTAGTAAGCATAGTTACC
TTCTTAGAGAAAATTGGTTTTGAGATTGACGTGGAGAAATCGCCAAAATA
TCTCAAAATACCAACAAAGCTAAAACCTGACATGACAATTAGATCTAAAA
GGAACAAGGAAATACATGTTCTTGATCTAAAGGTGCCTTATGACTCAGTT
AAGAGCTTTGATAAAGCGCGAGAAGCTAACTATGAAAAATACAAAGACCT
GGCCATTTAAATAGGAGAGGCCTTCAAAGAAAGGGCAACGGTGTCTGCCA
TAGTGATTGGATGTTTGGAAACTTGTGACAAGAAAAATAATCCAGCATTG
GCAAAGATTGGACTTACTAAACCAGAAATCAAAACCCTTGCCAAGATAGC
ATGTCCAAAAGCAGTAACTGCTTGCTACCACATCTACCGTGAGCACATCT
CATATACAAAGAGTGCCATTGTACTACCCTTCAGTCTTACCTAATTCAGG
ATATGCAGCAATGCTGGTAATTGCATCGGCGTTGCAGACTTATGTTGAAA
AGATAAAAAACTAACAGGTTGAAATGCTGAGCCACGCTCGCATACATAAG
CCAAAAGGCCGTGATTTTTTCGTTT
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protein sequence of SMED30021552-orf-1

>SMED30021552-orf-1 ID=SMED30021552-orf-1|Name=SMED30021552-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=473bp
MKSMISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYA
VDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAF
VVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFW
IGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCP
NVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYE
IKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAI
SHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFN
ERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNII
QLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIK
NISDPIRKNDTTEESIYETISQ*
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protein sequence of SMED30021552-orf-2

>SMED30021552-orf-2 ID=SMED30021552-orf-2|Name=SMED30021552-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=247bp
MNGEVSKQFPNTSFFKKFQKAALALKRKFCICFKLNFVDDTFSLEMSYKK
RISYINIQNLKTLSKELHDFVGLHYTEQWCQMHVRGHIATTIGDSITAKY
LVASDTLNDAQYTFLIRARNNILNLNYNPYRMKNNLDTKWRLCHLDNETQ
THVFNHCPAKPNARRIKHENVLVSIVTFLEKIGFEIDVEKSPKYLKIPTK
LKPDMTIRSKRNKEIHVLDLKVPYDSVKSFDKAREANYEKYKDLAI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003964RNA-directed DNA polymerase activity
Vocabulary: biological process
TermDefinition
GO:0006278RNA-dependent DNA biosynthetic process
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell