Nuclear autoantigenic sperm protein

Overview
NameNuclear autoantigenic sperm protein
Smed IDSMED30021509
Length (bp)1148
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Nuclear autoantigenic sperm protein (SMED30021509) t-SNE clustered cells

Violin plots show distribution of expression levels for Nuclear autoantigenic sperm protein (SMED30021509) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nuclear autoantigenic sperm protein (SMED30021509) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nuclear autoantigenic sperm protein (SMED30021509) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30021509

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30021509h1SMcG0020925 SmedASXL_000706SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30021509h1SMcG0020925 SmedASXL_000706SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
cephalic gangliaSMED30021509h1SMcG0020925 dd_Smed_v4_5663_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30021509h1SMcG0020925 dd_Smed_v4_5663_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30021509h1SMcG0020925 SMED_00777_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30021509h1SMcG0020925 SMED_00777_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Human
Match: NASP (nuclear autoantigenic sperm protein [Source:HGNC Symbol;Acc:HGNC:7644])

HSP 1 Score: 55.8398 bits (133), Expect = 6.076e-8
Identity = 74/363 (20.39%), Postives = 154/363 (42.42%), Query Frame = 3 
Query:   87 LFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILEN--TEKEPKKNIEQSNEVKKDKES-LSYLQLAYETMECAKLILKKQINEKTGNET-ELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV----SVCQXXXXXXXXXX---------LEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSS--VPAN------------------------DVTHLLRRK 1046
            L   G+K  ++GD   A+    +A       +G    +  E +F+  K+    ++ E  +LG  LE     E +         V    N+                      +   SE+   EN  TE+ P  ++ ++  +++++E  +  L+LA++ ++ AK+I K+Q       ET E +L  A     +G   +E     +++ E++  L  ++++LEA DR +AE+++ LG+ Y  + +  EA+  + K   ++    +V     + +             L++++ +I E+I D  E  +   +A+ +++  L+ +S+S   P                          D++HL+R+K
Sbjct:   46 LLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNID---------------------ETEGSEEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQ-------ETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKK 380
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Human
Match: NASP (nuclear autoantigenic sperm protein [Source:HGNC Symbol;Acc:HGNC:7644])

HSP 1 Score: 52.7582 bits (125), Expect = 6.702e-7
Identity = 51/237 (21.52%), Postives = 114/237 (48.10%), Query Frame = 3 
Query:  459 TEKEPKKNIEQSNEVKKDKES-LSYLQLAYETMECAKLILKKQINEKTGNET-ELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV----SVCQXXXXXXXXXX---------LEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSS--VPAN------------------------DVTHLLRRK 1046
            TE+ P  ++ ++  +++++E  +  L+LA++ ++ AK+I K+Q       ET E +L  A     +G   +E     +++ E++  L  ++++LEA DR +AE+++ LG+ Y  + +  EA+  + K   ++    +V     + +             L++++ +I E+I D  E  +   +A+ +++  L+ +S+S   P                          D++HL+R+K
Sbjct:  124 TEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQ-------ETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKK 353
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Human
Match: NASP (nuclear autoantigenic sperm protein [Source:HGNC Symbol;Acc:HGNC:7644])

HSP 1 Score: 51.9878 bits (123), Expect = 7.661e-7
Identity = 46/196 (23.47%), Postives = 102/196 (52.04%), Query Frame = 3 
Query:  510 DKESLSYLQLAYETMECAKLILKKQINEKTGNET-ELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV-------------SVCQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSS--VPANDVTHL-LRRK 1046
            ++E +  L+LA++ ++ AK+I K+Q       ET E +L  A     +G   +E     +++ E++  L  ++++LEA DR +AE+++ LG+ Y  + +  EA+  + K   ++             +   ++E     +EL++++ +I E+I D  E  +   +A+ +++  L+ +S+S   P    + + ++RK
Sbjct:   33 EEEEIGNLELAWDMLDLAKIIFKRQ-------ETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMKRK 221
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Human
Match: NASP (nuclear autoantigenic sperm protein [Source:HGNC Symbol;Acc:HGNC:7644])

HSP 1 Score: 50.447 bits (119), Expect = 2.181e-6
Identity = 34/132 (25.76%), Postives = 75/132 (56.82%), Query Frame = 3 
Query:  459 TEKEPKKNIEQSNEVKKDKES-LSYLQLAYETMECAKLILKKQINEKTGNET-ELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV 848
            TE+ P  ++ ++  +++++E  +  L+LA++ ++ AK+I K+Q       ET E +L  A     +G   +E     +++ E++  L  ++++LEA DR +AE+++ LG+ Y  + +  EA+  + K   ++
Sbjct:   87 TEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQ-------ETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVI 211
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Human
Match: NASP (nuclear autoantigenic sperm protein [Source:HGNC Symbol;Acc:HGNC:7644])

HSP 1 Score: 51.2174 bits (121), Expect = 2.186e-6
Identity = 48/219 (21.92%), Postives = 103/219 (47.03%), Query Frame = 3 
Query:  510 DKESLSYLQLAYETMECAKLILKKQINEKTGNET-ELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV----SVCQXXXXXXXXXX---------LEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSS--VPAN------------------------DVTHLLRRK 1046
            ++E +  L+LA++ ++ AK+I K+Q       ET E +L  A     +G   +E     +++ E++  L  ++++LEA DR +AE+++ LG+ Y  + +  EA+  + K   ++    +V     + +             L++++ +I E+I D  E  +   +A+ +++  L+ +S+S   P                          D++HL+R+K
Sbjct:  508 EEEEIGNLELAWDMLDLAKIIFKRQ-------ETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKK 719
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Zebrafish
Match: nasp (nuclear autoantigenic sperm protein (histone-binding) [Source:ZFIN;Acc:ZDB-GENE-030131-5511])

HSP 1 Score: 59.3066 bits (142), Expect = 2.357e-9
Identity = 76/327 (23.24%), Postives = 143/327 (43.73%), Query Frame = 3 
Query:   87 LFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILENTEKEPKKNIEQSNEVKKDKESLSYLQLAYETMECAKLILKKQIN-EKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV----SVCQ-----------XXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFL--MVTSSSVP 1013
            L   G +  ++GD   A+    +AC      +G    +  E +F+  KA    ++ E  +LG  LE   +  S++     + K+    NL   ++  + E+++ +   +   K S                          +++ +  LQLA+E +E AK+I K++ + E      ++ LKLA+    VG   +E G   +++ ++ + L  + KHL +  R +AE+++ LG  YS   +  +AIE +     ++    ++ Q             EE    +EL+ ++ +I E+I D  E  +    A E+I Q L    TSS+ P
Sbjct:   37 LIGTGSRHLVMGDVVSAVSVFQEACAMLAEKYGDTADECGEAFFFCGKALLELARMENTVLGNALEGVPEESSEEGEKQDDSKIESADNLDGDDDGDEDEDDDEDAEGDAKDKES--------------------------EEDEVGNLQLAWEMLEVAKVIYKRKDDKEDQLMAAQIHLKLAE----VG---VESGNYSQALEDFNECLTLQLKHLPSHSRLLAETHYQLGTTYSYTTQYNQAIEHFSNSIKVIESRLAMLQEVIDKAEGEDSAKEEKGEFEELKQLLPEIKEKIEDAKESQRTAAAASEAIHQTLAGASTSSAFP 330
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Zebrafish
Match: nasp (nuclear autoantigenic sperm protein (histone-binding) [Source:ZFIN;Acc:ZDB-GENE-030131-5511])

HSP 1 Score: 54.299 bits (129), Expect = 1.459e-7
Identity = 58/191 (30.37%), Postives = 97/191 (50.79%), Query Frame = 3 
Query:  507 KDKES----LSYLQLAYETMECAKLILKKQIN-EKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKC----KSIVSVCQ-----------XXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFL--MVTSSSVP 1013
            KDKES    +  LQLA+E +E AK+I K++ + E      ++ LKLA+    VG   +E G   +++ ++ + L  + KHL +  R +AE+++ LG  YS   +  +AIE +       +S +++ Q             EE    +EL+ ++ +I E+I D  E  +    A E+I Q L    TSS+ P
Sbjct:  344 KDKESEEDEVGNLQLAWEMLEVAKVIYKRKDDKEDQLMAAQIHLKLAE----VG---VESGNYSQALEDFNECLTLQLKHLPSHSRLLAETHYQLGTTYSYTTQYNQAIEHFSNSIKVIESRLAMLQEVIDKAEGEDAAKEEKGEFEELKQLLPEIKEKIEDAKESQRTAAAASEAIHQTLAGASTSSAFP 527
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Zebrafish
Match: nasp (nuclear autoantigenic sperm protein (histone-binding) [Source:ZFIN;Acc:ZDB-GENE-030131-5511])

HSP 1 Score: 53.9138 bits (128), Expect = 2.134e-7
Identity = 58/191 (30.37%), Postives = 97/191 (50.79%), Query Frame = 3 
Query:  507 KDKES----LSYLQLAYETMECAKLILKKQIN-EKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKC----KSIVSVCQ-----------XXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFL--MVTSSSVP 1013
            KDKES    +  LQLA+E +E AK+I K++ + E      ++ LKLA+    VG   +E G   +++ ++ + L  + KHL +  R +AE+++ LG  YS   +  +AIE +       +S +++ Q             EE    +EL+ ++ +I E+I D  E  +    A E+I Q L    TSS+ P
Sbjct:  347 KDKESEEDEVGNLQLAWEMLEVAKVIYKRKDDKEDQLMAAQIHLKLAE----VG---VESGNYSQALEDFNECLTLQLKHLPSHSRLLAETHYQLGTTYSYTTQYNQAIEHFSNSIKVIESRLAMLQEVIDKAEGEDSAKEEKGEFEELKQLLPEIKEKIEDAKESQRTAAAASEAIHQTLAGASTSSAFP 530
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Xenopus
Match: slc39a3 (solute carrier family 39 member 3 [Source:Xenbase;Acc:XB-GENE-969593])

HSP 1 Score: 63.1586 bits (152), Expect = 2.078e-10
Identity = 53/218 (24.31%), Postives = 100/218 (45.87%), Query Frame = 3 
Query:  510 DKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV-------------SVCQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFL----------------------MVTSSSVP----ANDVTHLLRRK 1046
            ++E +  LQLA+E ++  KLI K+Q         E +LK A     +G   +E     +++ ++   L  +++HLE  DR +AE+ ++LG+ Y    +  +AI  + +   ++             SV +  E      EL+D++ DI E+I D  E  K+    + ++++ L                      M+  S+VP     +D++HL+R+K
Sbjct:  127 EEEDVGNLQLAWEMLDLCKLIFKRQ------QSKEAQLKAAQAHLKLGEVSVESENYSQAVEDFLACLNIQKEHLEEHDRLLAETNYHLGLAYQYSSKHEDAISHFTQSVGVIEKRMGVLTKQLETSVGEPDEVKKEMDELKDLLPDIKEKIEDSKEAQKNATATEIALKETLVSIAIILLSVTLGFIAACSWLEMIGDSTVPVTNCVSDISHLVRKK 338
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Xenopus
Match: slc39a3 (solute carrier family 39 member 3 [Source:Xenbase;Acc:XB-GENE-969593])

HSP 1 Score: 60.4622 bits (145), Expect = 2.370e-9
Identity = 52/214 (24.30%), Postives = 99/214 (46.26%), Query Frame = 3 
Query:  522 LSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV-------------SVCQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTS----------------------SSVP----ANDVTHLLRRK 1046
            +  LQLA+E ++  KLI K+Q ++      E +LK A     +G   +E     +++ ++   L  +++HLE  DR +AE+ ++LG+ Y    +  +AI  + +   ++             SV +  E      EL+D++ DI E+I D  E  K+    + ++++ L+  S                      S+VP     +D++HL+R+K
Sbjct:  337 VGNLQLAWEMLDLCKLIFKRQQSK------EAQLKAAQAHLKLGEVSVESENYSQAVEDFLACLNIQKEHLEEHDRLLAETNYHLGLAYQYSSKHEDAISHFTQSVGVIEKRMGVLTKQLETSVGEPDEVKKEMDELKDLLPDIKEKIEDSKEAQKNATATEIALKETLVGGSSGFSQENGSTSASSTTVEKSGDSTVPVTNCVSDISHLVRKK 544
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Xenopus
Match: slc39a3 (solute carrier family 39 member 3 [Source:Xenbase;Acc:XB-GENE-969593])

HSP 1 Score: 60.4622 bits (145), Expect = 2.578e-9
Identity = 52/214 (24.30%), Postives = 99/214 (46.26%), Query Frame = 3 
Query:  522 LSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV-------------SVCQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTS----------------------SSVP----ANDVTHLLRRK 1046
            +  LQLA+E ++  KLI K+Q ++      E +LK A     +G   +E     +++ ++   L  +++HLE  DR +AE+ ++LG+ Y    +  +AI  + +   ++             SV +  E      EL+D++ DI E+I D  E  K+    + ++++ L+  S                      S+VP     +D++HL+R+K
Sbjct:  342 VGNLQLAWEMLDLCKLIFKRQQSK------EAQLKAAQAHLKLGEVSVESENYSQAVEDFLACLNIQKEHLEEHDRLLAETNYHLGLAYQYSSKHEDAISHFTQSVGVIEKRMGVLTKQLETSVGEPDEVKKEMDELKDLLPDIKEKIEDSKEAQKNATATEIALKETLVGGSSGFSQENGSTSASSTTVEKSGDSTVPVTNCVSDISHLVRKK 549
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Mouse
Match: Nasp (nuclear autoantigenic sperm protein (histone-binding) [Source:MGI Symbol;Acc:MGI:1355328])

HSP 1 Score: 51.6026 bits (122), Expect = 9.501e-7
Identity = 57/258 (22.09%), Postives = 112/258 (43.41%), Query Frame = 3 
Query:   87 LFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILENTEKEPKKN---IEQSNEVKKDKESLSYLQLAYETMECAKLILKKQINEKTGNET-ELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV 848
            L   G+K  ++GD   A+    +A       +G    +  E +F+  K+    ++ E  +LG  LE     E +         V    N+                      +   SE+   EN + E   N   +E+ +  + ++E +  L+LA++ ++ AK+I K+Q       ET E +L  A     +G   +E     +++ E++  L  ++++LEA DR +AE+++ LG+ Y  + +  EA+  + K   ++
Sbjct:   46 LLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNVD---------------------ETEGSEEEDRENDKAEETPNESVLEKKSLQENEEEEIGNLELAWDMLDLAKIIFKRQ-------ETKEAQLYAAQAHLKLGEVSVESENYIQAVEEFQACLSLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVI 275
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Mouse
Match: Nasp (nuclear autoantigenic sperm protein (histone-binding) [Source:MGI Symbol;Acc:MGI:1355328])

HSP 1 Score: 48.9062 bits (115), Expect = 7.271e-6
Identity = 31/114 (27.19%), Postives = 64/114 (56.14%), Query Frame = 3 
Query:  510 DKESLSYLQLAYETMECAKLILKKQINEKTGNET-ELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV 848
            ++E +  L+LA++ ++ AK+I K+Q       ET E +L  A     +G   +E     +++ E++  L  ++++LEA DR +AE+++ LG+ Y  + +  EA+  + K   ++
Sbjct:  142 EEEEIGNLELAWDMLDLAKIIFKRQ-------ETKEAQLYAAQAHLKLGEVSVESENYIQAVEEFQACLSLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVI 248
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. UniProt/SwissProt
Match: sp|Q02508|HGV2_HALRO (Protein HGV2 OS=Halocynthia roretzi OX=7729 GN=HGV2 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.574e-10
Identity = 67/249 (26.91%), Postives = 108/249 (43.37%), Query Frame = 3 
Query:  510 DKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAY-------------------------EKCKSIVSVCQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYE-LAKESIRQ------------------------FLMVTSSSVP-----ANDVTHLLRRKLSAPATEEAEPPISK 1091
            D + +S +QLA+E +E AK++ KK    KT  +      +A C   +G   +E     ++I ++ + L  ++  L  +DR +AE+Y+NLG+ YS + R   A+E Y                         EK K I+S C+         EL+++I DI+ +I D +   K  + L     RQ                           V  +SVP      +D+THL+RRK  +P  E+ +P  SK
Sbjct:  225 DPDDISNMQLAWEMLELAKVLYKKHDKSKTNKQM-----VAQCHLKLGELGLEVENHPQAIGDFLECLVIQKDLLPETDRKLAETYYNLGLAYSFEKRYDNALEHYQSALDVLEARVDMLNELIESNEGNKEKEKEIISECK------EVGELKELIPDINSKIEDVILAKKQMQKLDGSPFRQASEGESSSGLGASTSDDKPCSTIPIRKVAPTSVPVAKDSPSDITHLVRRKRPSP-DEDNQPAESK 461
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. UniProt/SwissProt
Match: sp|P06180|HIBN_XENLA (Histone-binding protein N1/N2 OS=Xenopus laevis OX=8355 PE=1 SV=3)

HSP 1 Score: 61.2326 bits (147), Expect = 7.847e-9
Identity = 56/225 (24.89%), Postives = 105/225 (46.67%), Query Frame = 3 
Query:  522 LSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV-------------SVCQ-XXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTS---------------------SSVP----ANDVTHLLRRKLSAPATEEAEP 1079
            +  LQLA+E ++  K I K+Q ++      E +LK A     +G   +E     +++ ++   L  +++HLE  DR +AE++++LG+ Y    +  EAI  + +   ++             SV +   E      EL+D++ DI E+I D  E  K+  + ++++++ L+  S                     S+VP     +D++HL+R+K     TEE  P
Sbjct:  327 VGNLQLAWEMLDLCKTIFKRQQSK------EAQLKAAQAHQKLGEVCIESENYSQAVEDFLACLNIQKEHLEEHDRLLAETHYHLGLAYQYSSKHEEAISHFTQSIGVIEKRMDVLTKQLEASVGELVDEVKKEMDELKDLLPDIKEKIEDSKEAQKNATVTEKALKETLVGGSSGFSKENGSTSSSSAVEKSGDSTVPVTNCVSDISHLVRKKRK---TEEESP 542
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. TrEMBL
Match: A0A482VSH6 (Nuclear autoantigenic sperm protein OS=Asbolus verrucosus OX=1661398 GN=BDFB_006606 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 8.939e-14
Identity = 95/342 (27.78%), Postives = 162/342 (47.37%), Query Frame = 3 
Query:   75 SSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGH-LHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNAD--SESKDVPISIND--KVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILENTEKEPKKNIEQSNEVKKDKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIE----AYEKCKSIVSVCQXXXXXXXXXX-----LEDIIADIDERITDC----MEGAKDYEL----AKESIRQFLMVTSSSVPANDVTHL 1034
            +S +LF QG + Y++ DY  A+    K  +     HG+ +H  + + Y Y  K+    S+ E+  LGEG+ KN D  SES+++  S +D  K      L  V+ + D  +      + KS++++       +  E+  K++I    E ++ +E L+ LQ+A+E +E AK I   +  +   N       LA+   ++G   +E G    +I +  + LE ++       R IAE+Y+ LG   S++ +  EAIE    +YE   + V+  +  EE+          L ++I ++ E+I D     ME AK        A  S       +SSS  AND++HL
Sbjct:   12 NSKELFGQGVRAYVLQDYDTAVRAFSKVSELLSAEHGNDMHDSLGDVYLYYGKSLLELSRKESGPLGEGVLKNVDEGSESEEIQESDDDEDKTETTNKLEEVKANEDNNQETEKTEEAKSAEDAAKTDD--KPAEEASKEDISPKKEGEESEEELTDLQVAWEVLELAKRIFINRGADGKKN-------LAETLIVLGEISLESGNFSSAIEDMTEGLEIQKTLFGNDSRTIAETYYKLGGALSTNGQIDEAIENFYASYEYLSNKVTSLKKDEEHKEANAEEIEELNNLIPEVQEKIADMKNLKMEVAKKVTAPVAEAAPSTNGTPEASSSSKQANDISHL 344
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. TrEMBL
Match: I6TH51 (Nuclear autoantigenic sperm protein OS=Spirometra erinaceieuropaei OX=99802 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.038e-14
Identity = 73/270 (27.04%), Postives = 122/270 (45.19%), Query Frame = 3 
Query:   75 SSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQG--------ILENTEKEPKKNIEQSN-----------------------EVKKDKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYS 791
            ++ +L  +GK+  I  +   A+E   KAC+     HG  H  +  P      A    +++E  +L E +  NA+S+  D  +   D+  EE    A      L      +   +++KN+N E+G        + E+ E    +N E +N                       E K+  E +S LQLA+E +E AK + +K  +      +E +LK+ADC + +     E+   E++I +  + LE R KH   +DR IAE++F LG+ YS
Sbjct:   18 TAEELIAKGKRDLICLEISSAVENFQKACELIASEHGDFHESLATPNLLYGTALLELARSENHILSEAV-GNAESDESDSEV---DEDTEEDPPEAKNPPEVLTGEAKGNTQTRATKNTNGEKGASLPVGNVVQESREGSTTENGEVANDSDAVRVESGAPEPSCAAENDLPENKEADEDVSTLQLAWEVVEVAKALFRKHTD------SEHQLKVADCLEKLAEISCEKEDHEQAINDLLECLEIRMKHAGENDRLIAETHFQLGVTYS 277
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. TrEMBL
Match: A0A430QGA1 (Nuclear autoantigenic sperm protein OS=Schistosoma bovis OX=6184 GN=DC041_0006538 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 5.799e-13
Identity = 103/399 (25.81%), Postives = 175/399 (43.86%), Query Frame = 3 
Query:   66 FEQSSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLE------KNADSESKDVPI------------SINDKVI---------EEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILENT-----------EKEPKKNIEQSNEVKKDKES-LSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVY--SSDLRSAEA-IEAYEKC---------KSIVSVCQXXXX-----XXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQF-----LMVTSSSVPANDVTHLLRRKLSAPATEEAEP 1079
             E +  DL  QG++  + GD  KA++   KAC++  + HG  H  +  P      A    S+ E+ ++G  L+      KN DSES D  I             I+++VI         EE      +  S+  E  S +     +   ++ +G  ++            + E + N  +S   K D E  +S LQ+A+E +E AK +  ++ +E++      +L +A+C + +G    E+   ++++ + ++ LE R   L   DR +AES++ LG  Y  S DL +A +  +A  +C           I +V Q  EE         KE+E +I D+  R  D  E     +   ESI+Q           +SV  +D++HL+R+K     T   EP
Sbjct:  320 MEHAPEDLITQGRRDLVCGDINKAVDNFQKACEALSMIHGDFHEALAIPNLLYGTALLELSRIESSVIGNALDGIPDSDKN-DSESDDGIIEPGPELTTEEKEDISNQVIDAMCEDKKQEESETKEADTPSEDTEAVSGNDAATDNPTDDASEGAEDDESNGSEEEESENQDEVQNNPTESEAAKSDDEDEVSNLQIAWEVIEVAKKLFSQKDDEES------KLNVAECLEKLGEISREKEDYDQAVLDLKECLEIRTSILGQKDRRVAESHYQLGTTYAVSGDLANASSSFKASVECLKLLAEDIRSKIEAVDQKDEEEKELLQVTLKEIELLIPDVQSRCADIEE-----DRLAESIKQTESPMEPCSNGTSVSTDDISHLIRKKRPVTETTPVEP 706
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. TrEMBL
Match: A0A068XXK0 (Egf domain protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000438900 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.017e-12
Identity = 91/353 (25.78%), Postives = 158/353 (44.76%), Query Frame = 3 
Query:   75 SSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVP-ISINDKVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILENTEKE-PKKNIEQSN----------EVKKD--KESLSYLQLAYETMECA-KLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNL-------GMVYSSDLRSAEAIEAYEKCKSIVSV------CQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFLMV-TSSSVPANDVTHLLRRK 1046
            S  +L  +GK+  +      A+EC   AC+     +G LH D+  P      A    S++E  +LGE +EK A  ES D P  S +D+   E+N+                      K +N+E+G+  N   +    ++E+S           E  +D   E +S LQLA+E +E A KL L+        ++ E +L+ +DC + +     E G  ++++++  + L+ R  H   +DR IAE++F L       G V SSD    +A+   E CK  + +       +   + S   +LE  I ++   I +  E  K+   + +S+ +         VP  D++HL+++K
Sbjct:   14 SPEELIAKGKREMLCSQISNAVECFQMACQLLAEKNGDLHDDLATPNLLYGTALLELSRSENNILGESMEKRA--ESMDSPEDSGDDEGDRERNIKG------------------ELKQANAEKGVGVNEVGDVGTSSVEKSTASGEATFSPPEGSEDLGDEDISTLQLAWEVVEVARKLFLRH-------DDAEHQLRASDCLEKLAEIGQEIGNHQQAVSDLLECLKIRLIHAPNNDRLIAETHFQLAVSYSQVGNVISSDASFVDALRRLEGCKLKLELQLSAINGEGEGDGSKINQLEMQIREVVGLIGEVSERRKEGSQSIQSVEEPQATPVHKDVPIGDISHLIKKK 339
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. TrEMBL
Match: A0A0R3VWQ4 (SHNi-TPR domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS1849 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.115e-12
Identity = 80/345 (23.19%), Postives = 155/345 (44.93%), Query Frame = 3 
Query:   75 SSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILENTEKEPKKNIEQSNEVKKDKES-LSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVY-------SSDLRSAEAIEAYEKCKSIVSV-------------CQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSSVPANDVTHLLRRK 1046
            S  +L  +GK+  +      A++C   AC+     +G LH D+  P      A    S++E  +LGE +E+  +S +     S  D   E+ +  A  +                S+  N     +E +    ++ +  + E++  ++  +S LQLA+E +E AK +  +        + E +LK +DC + +     E G  +++I++  + L+ R +H   +DR IAE++F L + Y       SSD    +A+    +CKS + +              + +      +E+ D+I ++ ER     EG +  +  +E+  + +    S VP +D+THL+++K
Sbjct:   14 SPEELIAKGKREMLCSQLPNAVDCFQMACQLLAEKNGDLHDDLAIPNLLYGTALLELSRSENHVLGESVERCGESAT-----SSED---EDGDDEARAKDFKESGQFITKESADVSEFGNVGASGVEKSSAPEEETVPPATELEDSRDGDVSTLQLAWEVVEVAKKLFMRH------EDAEHQLKASDCLEKLAEIGQEIGNHQQAISDLLECLKIRSEHAPDNDRLIAETHFQLAVSYSQVGDATSSDASFGDALRHLGECKSKLELQLSALDEEDVGENTKVNHLQMQIREVADLIGEVSERRK---EGPEPVQTVEETQAKPMQ---SDVPVDDITHLIKKK 338
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Cavefish
Match: nasp (nuclear autoantigenic sperm protein (histone-binding) [Source:ZFIN;Acc:ZDB-GENE-030131-5511])

HSP 1 Score: 51.2174 bits (121), Expect = 1.140e-6
Identity = 50/180 (27.78%), Postives = 87/180 (48.33%), Query Frame = 3 
Query:  522 LSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIV--------------SVCQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFL--MVTSSSVP 1013
            +  LQLA+E +E AK+I KK+  +      ++ LKL +    VG     E    +++ ++++ L  + KHL    R +AE+++ LG  +S   +   AIE + K  S++                 +  EE +  +EL+ ++ +I E++ D  E  K    A E+I Q L    TSS+ P
Sbjct:  315 VGNLQLAWEMLEVAKVIYKKESKDDQLMAAQIFLKLGE----VGA----ESNYPQALEDFKECLALQLKHLPPHSRLLAETHYQLGTTFSYTAQYNLAIEHFTKSISVIESRLMLQEVINKAEGAEEAKEEKNEFEELKQLLPEITEKVEDAKESQKTAAAASEAIHQTLGGASTSSAFP 486
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Medaka
Match: NASP (nuclear autoantigenic sperm protein [Source:NCBI gene;Acc:101155799])

HSP 1 Score: 63.5438 bits (153), Expect = 1.149e-10
Identity = 56/210 (26.67%), Postives = 103/210 (49.05%), Query Frame = 3 
Query:  516 ESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEA-----------YEKCKSIVSVCQXXXX----XXXXXXLEDIIADIDERITDCMEGAKDYELA----------------KESIRQFLM--VTSSSVPANDVTHLLRRK 1046
            E +  LQLA+E +E AK I K++      +  E +L  A     +G    E G   +++ ++++ L+ + KHLEA+ R +AE+++ LG+ YS +L+ + AIEA            EK + ++   +  E         +EL+ ++ +I E++ D  EG K  + A                  S+   L     +S+VP ++++HL+R+K
Sbjct:  211 EEVGNLQLAWEMLEVAKSIYKRK------DTKEAQLLAAQAHLKLGEVSAESGNYTQALEDFQECLKLQVKHLEANSRLLAETHYQLGLTYSLNLQYSPAIEALNNSISVIKSRLEKLEELMKTAEGPEALPDERKEMEELKALLPEIQEKVEDATEGLKLVKAAVGGDQDGGSLVNGGASSSSVNGTLSNGGQTSAVPVSNISHLVRKK 414
[Close]
BLAST of Nuclear autoantigenic sperm protein vs. Planmine SMEST
Match: SMESG000016858.1 (SMESG000016858.1)

HSP 1 Score: 644.425 bits (1661), Expect = 0.000e+0
Identity = 348/350 (99.43%), Postives = 348/350 (99.43%), Query Frame = 3 
Query:   54 MNENFEQSSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVXXXXXXXXXXXXXXXXKSSKNSNSEQGILENTEKEPKKNIEQSNEVKKDKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYEKCKSIVSVCQXXXXXXXXXXLEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSSVPANDVTHLLRRKLSAPATEEAEPPISKRKLE 1103
            MNENFEQSSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVEESSDLEENESNDGDEKSSKNSNSEQGILEN EKEPKKNIEQSNEVKKDKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAY KCKSIVSVCQNSEENSNKKELEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSSVPANDVTHLLRRKLSAPATEEAEPPISKRKLE
Sbjct:    1 MNENFEQSSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADMFEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKNLSAVEESSDLEENESNDGDEKSSKNSNSEQGILENAEKEPKKNIEQSNEVKKDKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVGNTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLRSAEAIEAYAKCKSIVSVCQNSEENSNKKELEDIIADIDERITDCMEGAKDYELAKESIRQFLMVTSSSVPANDVTHLLRRKLSAPATEEAEPPISKRKLE 350
The following BLAST results are available for this feature:
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NASP6.076e-820.39nuclear autoantigenic sperm protein [Source:HGNC S... [more]
NASP6.702e-721.52nuclear autoantigenic sperm protein [Source:HGNC S... [more]
NASP7.661e-723.47nuclear autoantigenic sperm protein [Source:HGNC S... [more]
NASP2.181e-625.76nuclear autoantigenic sperm protein [Source:HGNC S... [more]
NASP2.186e-621.92nuclear autoantigenic sperm protein [Source:HGNC S... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nasp2.357e-923.24nuclear autoantigenic sperm protein (histone-bindi... [more]
nasp1.459e-730.37nuclear autoantigenic sperm protein (histone-bindi... [more]
nasp2.134e-730.37nuclear autoantigenic sperm protein (histone-bindi... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
slc39a32.078e-1024.31solute carrier family 39 member 3 [Source:Xenbase;... [more]
slc39a32.370e-924.30solute carrier family 39 member 3 [Source:Xenbase;... [more]
slc39a32.578e-924.30solute carrier family 39 member 3 [Source:Xenbase;... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Nasp9.501e-722.09nuclear autoantigenic sperm protein (histone-bindi... [more]
Nasp7.271e-627.19nuclear autoantigenic sperm protein (histone-bindi... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q02508|HGV2_HALRO1.574e-1026.91Protein HGV2 OS=Halocynthia roretzi OX=7729 GN=HGV... [more]
sp|P06180|HIBN_XENLA7.847e-924.89Histone-binding protein N1/N2 OS=Xenopus laevis OX... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A482VSH68.939e-1427.78Nuclear autoantigenic sperm protein OS=Asbolus ver... [more]
I6TH519.038e-1427.04Nuclear autoantigenic sperm protein OS=Spirometra ... [more]
A0A430QGA15.799e-1325.81Nuclear autoantigenic sperm protein OS=Schistosoma... [more]
A0A068XXK01.017e-1225.78Egf domain protein OS=Echinococcus multilocularis ... [more]
A0A0R3VWQ41.115e-1223.19SHNi-TPR domain-containing protein OS=Taenia asiat... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nasp1.140e-627.78nuclear autoantigenic sperm protein (histone-bindi... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
NASP1.149e-1026.67nuclear autoantigenic sperm protein [Source:NCBI g... [more]
back to top
BLAST of Nuclear autoantigenic sperm protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000016858.10.000e+099.43SMESG000016858.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021509 ID=SMED30021509|Name=Nuclear autoantigenic sperm protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1148bp
GTTTTTAAAGCTAACTTGTTTAAATAATTATTTAACTCAAAATTTAATTA
AAAATGAATGAAAACTTTGAACAATCGTCAACCGACTTGTTTAATCAAGG
GAAGAAATTATACATAATTGGAGATTATGGAAAAGCTTTAGAATGTCTTA
TTAAGGCCTGCAAATCTCGTGAACTAACTCATGGTCATTTACATGCTGAT
ATGTTTGAGCCTTATTTTTATCTGGCAAAAGCATATTATATGTATAGTAA
AAATGAAACTGAGTTGTTAGGAGAGGGATTAGAAAAAAATGCAGATTCAG
AATCAAAGGATGTTCCAATTTCGATAAACGATAAGGTCATAGAAGAAAAA
AATCTTAGTGCTGTCGAAGAATCAAGTGATTTAGAAGAGAATGAAAGTAA
TGATGGAGATGAAAAGAGTTCAAAAAATTCAAATTCTGAACAAGGAATTT
TGGAAAATACTGAAAAGGAACCCAAGAAAAATATCGAACAATCAAATGAA
GTGAAAAAAGACAAAGAAAGTTTATCATATTTGCAGCTGGCATACGAAAC
GATGGAGTGTGCAAAATTGATATTGAAAAAGCAAATCAATGAAAAAACCG
GAAACGAAACTGAATTGGAATTGAAGCTTGCCGATTGCTGTGATTTGGTG
GGCAACACGTACATGGAAGAAGGCGTGATGGAGGAATCAATAGCCGAATA
CCGAAAAGCTCTCGAATACCGACAGAAACATTTAGAAGCGAGCGATCGGG
CAATCGCTGAGAGCTATTTCAATCTGGGAATGGTTTACTCGTCCGATCTG
CGGTCTGCAGAGGCGATCGAAGCGTATGAGAAATGCAAATCGATTGTGAG
CGTTTGTCAGAATTCTGAGGAGAATTCAAACAAAAAAGAGTTGGAAGATA
TCATTGCCGATATCGATGAGAGAATTACCGATTGTATGGAGGGCGCCAAA
GATTACGAATTAGCCAAGGAGTCCATCAGGCAATTTCTGATGGTGACGTC
ATCATCGGTACCTGCCAATGACGTCACGCATTTGCTAAGGCGTAAACTCA
GCGCTCCCGCAACTGAGGAAGCAGAACCGCCAATCAGCAAGCGGAAATTG
GAATAAGATTTTGATGTTATATAAATAGATTTTTGTTTCTGTTAAAAA
back to top

protein sequence of SMED30021509-orf-1

>SMED30021509-orf-1 ID=SMED30021509-orf-1|Name=SMED30021509-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=351bp
MNENFEQSSTDLFNQGKKLYIIGDYGKALECLIKACKSRELTHGHLHADM
FEPYFYLAKAYYMYSKNETELLGEGLEKNADSESKDVPISINDKVIEEKN
LSAVEESSDLEENESNDGDEKSSKNSNSEQGILENTEKEPKKNIEQSNEV
KKDKESLSYLQLAYETMECAKLILKKQINEKTGNETELELKLADCCDLVG
NTYMEEGVMEESIAEYRKALEYRQKHLEASDRAIAESYFNLGMVYSSDLR
SAEAIEAYEKCKSIVSVCQNSEENSNKKELEDIIADIDERITDCMEGAKD
YELAKESIRQFLMVTSSSVPANDVTHLLRRKLSAPATEEAEPPISKRKLE
*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0032991macromolecular complex
GO:0005654nucleoplasm
GO:0005737cytoplasm
GO:0043234protein complex
GO:0000790nuclear chromatin
GO:0005634nucleus
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002056medial muscle cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR019734TPR_repeat
IPR011990TPR-like_helical_dom_sf
IPR013026TPR-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0032403protein complex binding
GO:0042393histone binding
GO:0051879Hsp90 protein binding
Vocabulary: biological process
TermDefinition
GO:0006260DNA replication
GO:0006334nucleosome assembly
GO:0006335DNA replication-dependent nucleosome assembly
GO:0006336DNA replication-independent nucleosome assembly
GO:0006810transport
GO:0007049cell cycle
GO:0008283cell proliferation
GO:0008584male gonad development
GO:0015031protein transport
GO:0033574response to testosterone
GO:0043486histone exchange
GO:0001824blastocyst development
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 270..290
NoneNo IPR availablePFAMPF13424TPR_12coord: 199..261
e-value: 1.6E-7
score: 31.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..150
NoneNo IPR availablePANTHERPTHR15081NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATEDcoord: 6..347
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 9..42
e-value: 30.0
score: 10.8
coord: 193..226
e-value: 8.9
score: 15.3
coord: 235..268
e-value: 12.0
score: 14.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3DG3DSA:1.25.40.10coord: 103..298
e-value: 1.3E-13
score: 52.7
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILYSSF48452TPR-likecoord: 13..259
IPR013026Tetratricopeptide repeat-containing domainPROSITEPS50293TPR_REGIONcoord: 200..268
score: 9.328