SMED30021504
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021504 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of SMED30021504 vs. Planmine SMEST
Match: SMESG000030479.1 (SMESG000030479.1) HSP 1 Score: 877.085 bits (2265), Expect = 0.000e+0 Identity = 448/451 (99.33%), Postives = 448/451 (99.33%), Query Frame = 3 Query: 108 MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDIXXXXXXXXXXXXXXXSQASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYIIDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDKTLRDRFSKEHNFIIERLENIRTNSSNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKFEHNGISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETISTD 1460 MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYI DFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSID TLRDRFSKEHNFIIERLENIRTNSSNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKF HNGISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETISTD Sbjct: 1 MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYINDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDNTLRDRFSKEHNFIIERLENIRTNSSNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKFAHNGISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETISTD 451
BLAST of SMED30021504 vs. Planmine SMEST
Match: SMESG000030479.1 (SMESG000030479.1) HSP 1 Score: 860.907 bits (2223), Expect = 0.000e+0 Identity = 441/444 (99.32%), Postives = 441/444 (99.32%), Query Frame = 3 Query: 129 MTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDIXXXXXXXXXXXXXXXSQASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYIIDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDKTLRDRFSKEHNFIIERLENIRTNSSNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKFEHNGISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETISTD 1460 MTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYI DFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSID TLRDRFSKEHNFIIERLENIRTNSSNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKF HNGISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETISTD Sbjct: 1 MTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDILRLLNLFLKTNKNLRSQASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYINDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDNTLRDRFSKEHNFIIERLENIRTNSSNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKFAHNGISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETISTD 444
BLAST of SMED30021504 vs. Planmine SMEST
Match: SMESG000030475.1 (SMESG000030475.1) HSP 1 Score: 260.381 bits (664), Expect = 4.706e-81 Identity = 168/463 (36.29%), Postives = 269/463 (58.10%), Query Frame = 3 Query: 108 MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDIXXXXXXXXXXXXXXXSQASKSQSIMQFLLKRLSSREKWETVDKIKY----------DVNLSSYLLLTRASQENTESIRRIVRQILEELKNDKNLQKRILYMIFYSSAEINAGFQKKELGLE-AENNGIMVKEILGDFVEMIRDYIIDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDKTLRDRFSKEHNFIIERLEN-IRTNS-SNLVRNGAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKFEHN--GISDWGSLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETI 1451 MA N ++ + ++ S ++ + +T T+ L++N LIYS +N+ FER GL L C+ L +Q +RD+LRLL+LF+KTN NL++QA S+ M++L L + W KY D N+ Y T +IR+IVRQ ++EL +N +++Y++FY+ + + + + + A+ + I+ KEI+G F++++RDY+ DFK FY + D +++L Y K+E++FDQIC+ I+Y+A +K+R ++++SG Y+L S IF +K +P FL + L+IF+IP+ID+ ++ +F + I +RL N IR S S L++N L + Y I K + N LITLP+ + N I WGSLDVD WLSE+NL+ R F+ +T RG Q+ L + DGK +I+LL++KL+I +I + E LGALET+ Sbjct: 1 MATQNLQLQATSLMDEIHNMSQSMNHSNMCKTIKKFTELLYSNRYLIYSNINEYFERIGLTGNLIKCLAQLPDQSKRDVLRLLHLFIKTNANLQNQAGMSEDFMKYLSDTLEKVDTWNNTKNFKYHIHIAAHIIIDSNVPRYF--------KTNTIRQIVRQKIDELMFSEN---KLIYIVFYACSIRISSLEDVSISPDDAKADLIVSKEIIGIFIDVVRDYLDDFKTFYKDADFASNQYLLYRVKSEFSFDQICRTIIYFAVQSEKLRMNIFESGFYDLKSNIFIIKYVPNEFLQNTLEIFKIPTIDQQIKQKFIAGYQRIFDRLSNMIRNGSVSTLIKNSVLNLFDLY---------GIPK---PSSNVLITLPSNTNSKDSNFLSIDRWGSLDVDNWLSENNLEEYRNKFSGLTGRGLKQMKKLTMDEDGKMLIIDLLSTKLDISIIMIFEFLGALETL 440
BLAST of SMED30021504 vs. Planmine SMEST
Match: SMESG000030473.1 (SMESG000030473.1) HSP 1 Score: 157.918 bits (398), Expect = 1.850e-42 Identity = 125/462 (27.06%), Postives = 232/462 (50.22%), Query Frame = 3 Query: 108 MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRVNDRFERSGLMLELFSCMDNLNEQFRRDIXXXXXXXXXXXXXXXSQASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTE--SIRRIVR-QILEELKNDKNLQKRILYM----IFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYIIDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDKTLRDRFSKEHNFIIERLENIRTNSSNLVRN--GAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKFEHNGISDWG----SLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETIS 1454 MA ++ + Y+ ++ S + + + +L++N ++YS +N F + GL+ L + ++ + +D+LRLL++F+KTNKN++ +S+ +++L L + W + +Y +++++YLLL + ++ + +IR I+R +I E++ ++ N + Y I S+A N + + +G F +I Y +FK FY N++N +++ + EY FDQIC I YY++ +++ +++ S +YE S I +++IPE F+ L I IPSI +R KE N I L + N S N + L KY +S D + + L+++ +E + I + SL VD+WL ++ L R PF ++ +G Q+ L+ D + +LIELLT+KL++D+I LE L L+ ++ Sbjct: 1 MALNSAQTKAIAYIDEIHSLSRSNRFTLMLSKINNLYIFLYSNKSILYSNINRYFYKIGLVPILTNYCHYISHETMKDVLRLLHIFIKTNKNIQEAVGQSEKFVKYLSAILCTESAWNNIGTFRYHIHIAAYLLLDQTCWQHFDIATIRAIIRGKIHEDIHSENNFLHTVCYACTMPITSSAASGNTSLTS------VTDEDLFSLSAMGGFSPLINSYFEEFKTFYKNSENTTNEYSLFLLDTEYAFDQICHSISYYSRLSERICANIFNSDIYESISGILAIRNIPEHFISRSLHILSIPSIYSHPDERIRKETNQISYALTSYFDNDSLPSDNQDSGKVLPNKYWSYESGSLYDTDDFSMKGKHTLLSI-ESENNLKSKSIGEKSTVKMSLTVDEWLIKNRLYYLRAPFDQLSEKGLYQMKTLITTPDNRIRLIELLTTKLDLDIIGTLEFLECLDRLT 455
BLAST of SMED30021504 vs. Planmine SMEST
Match: SMESG000030473.1 (SMESG000030473.1) HSP 1 Score: 142.51 bits (358), Expect = 1.862e-37 Identity = 101/366 (27.60%), Postives = 180/366 (49.18%), Query Frame = 3 Query: 396 ASKSQSIMQFLLKRLSSREKWETVDKIKYDVNLSSYLLLTRASQENTE--SIRRIVR-QILEELKNDKNLQKRILYM----IFYSSAEINAGFQKKELGLEAENNGIMVKEILGDFVEMIRDYIIDFKIFYDNTDNEQSKFLTYNYKAEYTFDQICQCIVYYAKTCKKVREDLYQSGLYELASTIFTMKSIPEVFLLSLLDIFEIPSIDKTLRDRFSKEHNFIIERLENIRTNSSNLVRN--GAERLIEKYRDCQSLKSKDISKQFPQTDNKLITLPNTEQKFEHNGISDWG----SLDVDQWLSEHNLQACRKPFTSMTSRGFTQLMFLMKENDGKFKLIELLTSKLNIDLISVLELLGALETIS 1454 +S+ +++L L + W + +Y +++++YLLL + ++ + +IR I+R +I E++ ++ N + Y I S+A N + + +G F +I Y +FK FY N++N +++ + EY FDQIC I YY++ +++ +++ S +YE S I +++IPE F+ L I IPSI +R KE N I L + N S N + L KY +S D + + L+++ +E + I + SL VD+WL ++ L R PF ++ +G Q+ L+ D + +LIELLT+KL++D+I LE L L+ ++ Sbjct: 22 VGQSEKFVKYLSAILCTESAWNNIGTFRYHIHIAAYLLLDQTCWQHFDIATIRAIIRGKIHEDIHSENNFLHTVCYACTMPITSSAASGNTSLTS------VTDEDLFSLSAMGGFSPLINSYFEEFKTFYKNSENTTNEYSLFLLDTEYAFDQICHSISYYSRLSERICANIFNSDIYESISGILAIRNIPEHFISRSLHILSIPSIYSHPDERIRKETNQISYALTSYFDNDSLPSDNQDSGKVLPNKYWSYESGSLYDTDDFSMKGKHTLLSI-ESENNLKSKSIGEKSTVKMSLTVDEWLIKNRLYYLRAPFDQLSEKGLYQMKTLITTPDNRIRLIELLTTKLDLDIIGTLEFLECLDRLT 380 The following BLAST results are available for this feature:
BLAST of SMED30021504 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30021504 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30021504 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30021504 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30021504 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021504 ID=SMED30021504|Name=SMED30021504|organism=Schmidtea mediterranea sexual|type=transcript|length=1602bpback to top protein sequence of SMED30021504-orf-1 >SMED30021504-orf-1 ID=SMED30021504-orf-1|Name=SMED30021504-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=452bp MAFSNCEMTGERYVSDLQEASSKKDYKALSETATTMTKYLFTNVDLIYSRback to top Annotated Terms
The following terms have been associated with this transcript:
|