SMED30021475

Overview
NameSMED30021475
Smed IDSMED30021475
Length (bp)1020
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30021475 (SMED30021475) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021475 (SMED30021475) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021475 (SMED30021475) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021475 (SMED30021475) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021475

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30021475h1SMcG0021275 SmedASXL_005544SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30021475h1SMcG0021275 dd_Smed_v4_18258_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021475h1SMcG0021275 dd_Smed_v6_18258_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30021475 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003395:22320..25318 +4971
Homology
BLAST of SMED30021475 vs. Ensembl Human
Match: HSD17B12 (hydroxysteroid 17-beta dehydrogenase 12 [Source:HGNC Symbol;Acc:HGNC:18646])

HSP 1 Score: 173.711 bits (439), Expect = 3.017e-51
Identity = 84/231 (36.36%), Postives = 139/231 (60.17%), Query Frame = -3
Query:  164 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHS 856
            G W VVTG+TDGIGK + E+LAK    ++LISR+++KL   S E+ EKF++  + I  DF    IYD I   + GL +G+L+NNVG +Y  P+++      D     M++ N LS   MT+++L   + +  G I+N+SS S  L +P+LT+Y++ K+ ++ F+ CL  E  ++G    +FVQ+  P++V +KL+ I+      P+ E F  S ++ +    ++ GY+ H+
Sbjct:   50 GEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKG----VFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHA 276          
BLAST of SMED30021475 vs. Ensembl Human
Match: HSDL1 (hydroxysteroid dehydrogenase like 1 [Source:HGNC Symbol;Acc:HGNC:16475])

HSP 1 Score: 163.31 bits (412), Expect = 4.894e-47
Identity = 89/263 (33.84%), Postives = 152/263 (57.79%), Query Frame = -3
Query:  122 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSG----IKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL 895
            P   S+ D IK YG W VV+G TDGIGK + E+LA R  N+ILISRNEEKL + ++++ + +++    I ADF   + IY  I E+++   VG+L+NNVG  YP P ++T   LS+    D+++ N  +  LM  ++L   + ++ G I+ +SS S     P L  +++ K+ L+ F+  LQ E  ++G    IFVQ+  PF+V + ++     +    +LVP+ + +    +  L  + ++ GY +HS + +   + +P++L
Sbjct:   54 PRLGSRADLIKQYGRWAVVSGATDGIGKAYAEELASRGLNIILISRNEEKLQVVAKDIADTYKVETDIIVADFSSGREIYLPIREALKDKDVGILVNNVGVFYPYPQYFT--QLSEDKLWDIINVNIAAASLMVHVVLPGMVERKKGAIVTISSGSCCKPTPQLAAFSASKAYLDHFSRALQYEYASKG----IFVQSLIPFYVATSMTAPSNFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWSHSIQ-FLFAQYMPEWL 309          
BLAST of SMED30021475 vs. Ensembl Human
Match: HSD17B3 (hydroxysteroid 17-beta dehydrogenase 3 [Source:HGNC Symbol;Acc:HGNC:5212])

HSP 1 Score: 152.91 bits (385), Expect = 2.841e-43
Identity = 89/249 (35.74%), Postives = 138/249 (55.42%), Query Frame = -3
Query:  125 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFID-MLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKF 868
            ++S G W V+TG  DGIGK +  +LAKR  N++LISR  EKL   + E+      SVK I ADF K  IY+HI E + GL +G+L+NNVG     P+   +  L+  D I  ++HCN  S V MT+++LK    +Q G+I+N+SS  +    P+ +MY++ K+ +  F+  LQ E  A+     + +Q  TP+ V + ++       +  TA+EF    L  +    ++ G + H     FL+ LIP +
Sbjct:   44 LRSMGQWAVITGAGDGIGKAYSFELAKRGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGML---PNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLILNISSGIALFPWPLYSMYSASKAFVCAFSKALQEEYKAK----EVIIQVLTPYAVSTAMTKYLNTNVITKTADEFVKESLNYVTIGGETCGCLAHEILAGFLS-LIPAW 284          
BLAST of SMED30021475 vs. Ensembl Human
Match: HSD17B3 (hydroxysteroid 17-beta dehydrogenase 3 [Source:HGNC Symbol;Acc:HGNC:5212])

HSP 1 Score: 149.828 bits (377), Expect = 2.692e-42
Identity = 84/235 (35.74%), Postives = 131/235 (55.74%), Query Frame = -3
Query:  167 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFID-MLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITH 868
            ++S G W V+TG  DGIGK +  +LAKR  N++LISR  EKL   + E+      SVK I ADF K  IY+HI E + GL +G+L+NNVG     P+   +  L+  D I  ++HCN  S V MT+++LK    +Q G+I+N+SS  +    P+ +MY++ K+ +  F+  LQ E  A+     + +Q  TP+ V + ++       +  TA+EF    L  +    ++ G + H
Sbjct:   44 LRSMGQWAVITGAGDGIGKAYSFELAKRGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGML---PNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLILNISSGIALFPWPLYSMYSASKAFVCAFSKALQEEYKAK----EVIIQVLTPYAVSTAMTKYLNTNVITKTADEFVKESLNYVTIGGETCGCLAH 271          
BLAST of SMED30021475 vs. Ensembl Human
Match: HSD17B3 (hydroxysteroid 17-beta dehydrogenase 3 [Source:HGNC Symbol;Acc:HGNC:5212])

HSP 1 Score: 137.502 bits (345), Expect = 6.754e-38
Identity = 75/194 (38.66%), Postives = 112/194 (57.73%), Query Frame = -3
Query:  290 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFID-MLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFW 868
            ++S G W V+TG  DGIGK +  +LAKR  N++LISR  EKL   + E+      SVK I ADF K  IY+HI E + GL +G+L+NNVG     P+   +  L+  D I  ++HCN  S V MT+++LK    +Q G+I+N+SS  +    P+ +MY++ K+ +  F+  LQ E  A+    +    +  P W
Sbjct:   44 LRSMGQWAVITGAGDGIGKAYSFELAKRGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGML---PNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLILNISSGIALFPWPLYSMYSASKAFVCAFSKALQEEYKAKEVIIQAGFLSLIPAW 234          
BLAST of SMED30021475 vs. Ensembl Celegans
Match: stdh-1 (Putative steroid dehydrogenase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q17703])

HSP 1 Score: 142.124 bits (357), Expect = 1.588e-39
Identity = 83/275 (30.18%), Postives = 142/275 (51.64%), Query Frame = -3
Query:   56 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFR-ISVKYITADFRKTKI--YDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 871
            K K+  +W VVTG TDGIGK +  +LAKR FN+ ++SR + KL    +E++E    I V++ T DF    +  Y+ +   +  + +G+LINNVG  +  P+     +       ++   N L   L++  +L   + ++ G+I+N+ S +    +   ++Y++ K  +   T CLQ E   QG    I  Q  TP  V +K++G     F  P ++ F  S L  +    Q+ GYITH  E   L KL+P F+    ++++  +LR+  ++  +K
Sbjct:   42 KKKAGASWAVVTGATDGIGKSYSFELAKRGFNVYIVSRTQSKLEHTKKEILEVHPDIEVRFATFDFTNPSVSDYEKLLSKLNEVSIGILINNVGMFFDYPEMLHKINGGIDSIANVTIINTLPATLLSAGILPQMVPRKAGIIVNIGSVAGLATMAEWSVYSATKKYVEWITGCLQKEYGHQG----IIFQAITPAMVATKMAGNPNTSFFTPDSDTFAKSALNTIGHASQTTGYITHQIECEML-KLLPDFVIDRSIKQTSAQLREALAKNENK 311          
BLAST of SMED30021475 vs. Ensembl Celegans
Match: let-767 (Very-long-chain 3-oxooacyl-coA reductase let-767 [Source:UniProtKB/Swiss-Prot;Acc:Q09517])

HSP 1 Score: 141.739 bits (356), Expect = 2.584e-39
Identity = 84/276 (30.43%), Postives = 145/276 (52.54%), Query Frame = -3
Query:   56 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKF-RISVKYITADFRKT--KIYDHIAESVEGLPVGLLINNVGAAYPSPD-FYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 871
            K ++  +W VVTG TDGIGK +  +LA+R FN++L+SR + KL    +E++EK+  I V+    DF       Y  +  ++  + +G+LINNVG +Y  PD  +  D   +R   ++   N L   L++  +L   + ++ GVI+N+ S++   ++ +  +Y++ K  ++  T  L+ E + QG    I VQ   P  V +K+S +K   F  P    F  S L  +     + GYITH  +   ++ LIP F+   ++    +  R  A RK+++
Sbjct:   42 KKRAGASWAVVTGATDGIGKAYAFELARRGFNVLLVSRTQSKLDETKKEILEKYSSIEVRTAAFDFTNAAPSAYKDLLATLNQVEIGVLINNVGMSYEYPDVLHKVDGGIER-LANITTINTLPPTLLSAGILPQMVARKAGVIVNVGSSAGANQMALWAVYSATKKYVSWLTAILRKEYEHQG----ITVQTIAPMMVATKMSKVKRTSFFTPDGAVFAKSALNTVGNTSDTTGYITHQLQLELMD-LIPTFIRDKILTNMSVGTRAAALRKKER 311          
BLAST of SMED30021475 vs. Ensembl Celegans
Match: let-767 (Very-long-chain 3-oxooacyl-coA reductase let-767 [Source:UniProtKB/Swiss-Prot;Acc:Q09517])

HSP 1 Score: 141.739 bits (356), Expect = 2.584e-39
Identity = 84/276 (30.43%), Postives = 145/276 (52.54%), Query Frame = -3
Query:   56 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKF-RISVKYITADFRKT--KIYDHIAESVEGLPVGLLINNVGAAYPSPD-FYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 871
            K ++  +W VVTG TDGIGK +  +LA+R FN++L+SR + KL    +E++EK+  I V+    DF       Y  +  ++  + +G+LINNVG +Y  PD  +  D   +R   ++   N L   L++  +L   + ++ GVI+N+ S++   ++ +  +Y++ K  ++  T  L+ E + QG    I VQ   P  V +K+S +K   F  P    F  S L  +     + GYITH  +   ++ LIP F+   ++    +  R  A RK+++
Sbjct:   42 KKRAGASWAVVTGATDGIGKAYAFELARRGFNVLLVSRTQSKLDETKKEILEKYSSIEVRTAAFDFTNAAPSAYKDLLATLNQVEIGVLINNVGMSYEYPDVLHKVDGGIER-LANITTINTLPPTLLSAGILPQMVARKAGVIVNVGSSAGANQMALWAVYSATKKYVSWLTAILRKEYEHQG----ITVQTIAPMMVATKMSKVKRTSFFTPDGAVFAKSALNTVGNTSDTTGYITHQLQLELMD-LIPTFIRDKILTNMSVGTRAAALRKKER 311          
BLAST of SMED30021475 vs. Ensembl Celegans
Match: let-767 (Very-long-chain 3-oxooacyl-coA reductase let-767 [Source:UniProtKB/Swiss-Prot;Acc:Q09517])

HSP 1 Score: 141.354 bits (355), Expect = 4.568e-39
Identity = 84/276 (30.43%), Postives = 145/276 (52.54%), Query Frame = -3
Query:   56 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKF-RISVKYITADFRKT--KIYDHIAESVEGLPVGLLINNVGAAYPSPD-FYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 871
            K ++  +W VVTG TDGIGK +  +LA+R FN++L+SR + KL    +E++EK+  I V+    DF       Y  +  ++  + +G+LINNVG +Y  PD  +  D   +R   ++   N L   L++  +L   + ++ GVI+N+ S++   ++ +  +Y++ K  ++  T  L+ E + QG    I VQ   P  V +K+S +K   F  P    F  S L  +     + GYITH  +   ++ LIP F+   ++    +  R  A RK+++
Sbjct:   59 KKRAGASWAVVTGATDGIGKAYAFELARRGFNVLLVSRTQSKLDETKKEILEKYSSIEVRTAAFDFTNAAPSAYKDLLATLNQVEIGVLINNVGMSYEYPDVLHKVDGGIER-LANITTINTLPPTLLSAGILPQMVARKAGVIVNVGSSAGANQMALWAVYSATKKYVSWLTAILRKEYEHQG----ITVQTIAPMMVATKMSKVKRTSFFTPDGAVFAKSALNTVGNTSDTTGYITHQLQLELMD-LIPTFIRDKILTNMSVGTRAAALRKKER 328          
BLAST of SMED30021475 vs. Ensembl Celegans
Match: stdh-3 (Putative steroid dehydrogenase 3 [Source:UniProtKB/Swiss-Prot;Acc:Q17704])

HSP 1 Score: 135.961 bits (341), Expect = 3.001e-37
Identity = 83/274 (30.29%), Postives = 141/274 (51.46%), Query Frame = -3
Query:   59 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKF-RISVKYITADFRKTKIYDH--IAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRD 871
            K K+  +W V+TG TDGIGK F  +LAKR FN+ ++SR + KL    +E+MEK+  + V++ T DF    I D+  +   +  + +G+LINNVG  +  P+             ++   N L   L++  +L   + ++ G+I+N+ S +   ++   ++Y++ K  +   T CL+ E + QG    I +Q  TP  V +KLSG        P +  F  S L  +    Q+ GYI H  +   L  L P+      V++S ++ R++A  K++
Sbjct:   42 KKKAGASWAVITGGTDGIGKSFSFELAKRGFNIYIVSRTQSKLEQTKKEIMEKYSNVEVRFATFDFTNPSISDYKKLLSQLNEVSIGMLINNVGMLFEYPENLHKTVGGIDVVANVTILNTLPVTLLSAGILPQMVSRKTGIIVNIGSVAGAAKMAEWSVYSASKKYVEWLTGCLRKEYEHQG----IIIQAITPALVATKLSGHTETSLFCPDSATFAKSALNTVGHTSQTTGYINHQIQCEML-ALFPECFLDSFVKKSSVETREKALAKKE 310          
BLAST of SMED30021475 vs. Ensembl Fly
Match: spidey (gene:FBgn0029975 transcript:FBtr0071091)

HSP 1 Score: 164.851 bits (416), Expect = 5.352e-48
Identity = 91/272 (33.46%), Postives = 155/272 (56.99%), Query Frame = -3
Query:   56 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKT-KIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFI-DMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENW---FLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 856
            G W VVTG+TDGIGK + ++LA+R   L+LISR+ EKL + ++E+ +K+ + V+ I  DF    +IYD I E   GL VG+L+NNVG +Y  P+++   + +D  F+ +++  N  S   MT + L   + ++ GVIIN+SS +  +  P+L++Y+S K+ +N F++ LQ E    G    I +Q+  P +V + +S I+      P+ E +  S L  L    Q+ GY+ H+       F   +  +   + +V ++++  R +A R+  K
Sbjct:   52 GEWAVVTGSTDGIGKAYAKELARRGLKLVLISRSLEKLNVVAKEIGDKYGVEVRVIDVDFTGGDEIYDKIREKTTGLNVGVLVNNVGISYGHPEYFLDCYKADPPFLRNIVAANIHSVTHMTALFLPGMISQRRGVIINVSSTAGVIPNPLLSVYSSTKAFVNKFSDDLQTEYKEHG----ILIQSVQPGFVATNMSKIRKASVFAPSPETYVRSALSTLGIATQTAGYLPHALLQLVIHFTEAVFGEQFARNIVMKNILGTRKRALRRLAK 319          
BLAST of SMED30021475 vs. Ensembl Fly
Match: CG13284 (gene:FBgn0032614 transcript:FBtr0345747)

HSP 1 Score: 157.532 bits (397), Expect = 3.643e-45
Identity = 88/276 (31.88%), Postives = 153/276 (55.43%), Query Frame = -3
Query:   56 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTR----KFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 868
            +  +G W VVTG TDGIGK +  +LA++  NL+LISR +EKL+  + E+  ++++  K+I ADF K + +YD I + + G+ VG+L+NNVG  Y  P+  + D +S+    ++L  N  S  ++TR +L   + ++ G I+N+ S+S    +P +T+YA+ K  +  F+  L+ E+    A   I VQ   P +V++K++    R        P A  F  S +  L +  ++ G+ TH  + + + KL P  +   L  +   +LR +A  ++ K
Sbjct:   52 VDKFGQWAVVTGATDGIGKEYARELARQGINLVLISRTKEKLIAVTNEIESQYKVKTKWIAADFAKGREVYDQIEKELAGIDVGILVNNVGMMYEHPE--SLDLVSEDLLWNLLTVNMGSVTMLTRKILPQMIGRRKGAIVNLGSSSELQPLPNMTVYAASKKFVTYFSKALELEV----AEHNIHVQLVMPNFVVTKMNAYTDRVMQGGLFFPNAYTFARSAVFTLGKTSETNGFWTHGIQ-YAIMKLAPLPIRTYLGHQLFKRLRIEALEQKQK 320          
BLAST of SMED30021475 vs. Ensembl Fly
Match: CG13284 (gene:FBgn0032614 transcript:FBtr0080926)

HSP 1 Score: 157.532 bits (397), Expect = 3.643e-45
Identity = 88/276 (31.88%), Postives = 153/276 (55.43%), Query Frame = -3
Query:   56 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTR----KFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 868
            +  +G W VVTG TDGIGK +  +LA++  NL+LISR +EKL+  + E+  ++++  K+I ADF K + +YD I + + G+ VG+L+NNVG  Y  P+  + D +S+    ++L  N  S  ++TR +L   + ++ G I+N+ S+S    +P +T+YA+ K  +  F+  L+ E+    A   I VQ   P +V++K++    R        P A  F  S +  L +  ++ G+ TH  + + + KL P  +   L  +   +LR +A  ++ K
Sbjct:   52 VDKFGQWAVVTGATDGIGKEYARELARQGINLVLISRTKEKLIAVTNEIESQYKVKTKWIAADFAKGREVYDQIEKELAGIDVGILVNNVGMMYEHPE--SLDLVSEDLLWNLLTVNMGSVTMLTRKILPQMIGRRKGAIVNLGSSSELQPLPNMTVYAASKKFVTYFSKALELEV----AEHNIHVQLVMPNFVVTKMNAYTDRVMQGGLFFPNAYTFARSAVFTLGKTSETNGFWTHGIQ-YAIMKLAPLPIRTYLGHQLFKRLRIEALEQKQK 320          
BLAST of SMED30021475 vs. Ensembl Fly
Match: CG13284 (gene:FBgn0032614 transcript:FBtr0332333)

HSP 1 Score: 157.147 bits (396), Expect = 6.281e-45
Identity = 88/276 (31.88%), Postives = 153/276 (55.43%), Query Frame = -3
Query:   56 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTR----KFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 868
            +  +G W VVTG TDGIGK +  +LA++  NL+LISR +EKL+  + E+  ++++  K+I ADF K + +YD I + + G+ VG+L+NNVG  Y  P+  + D +S+    ++L  N  S  ++TR +L   + ++ G I+N+ S+S    +P +T+YA+ K  +  F+  L+ E+    A   I VQ   P +V++K++    R        P A  F  S +  L +  ++ G+ TH  + + + KL P  +   L  +   +LR +A  ++ K
Sbjct:   65 VDKFGQWAVVTGATDGIGKEYARELARQGINLVLISRTKEKLIAVTNEIESQYKVKTKWIAADFAKGREVYDQIEKELAGIDVGILVNNVGMMYEHPE--SLDLVSEDLLWNLLTVNMGSVTMLTRKILPQMIGRRKGAIVNLGSSSELQPLPNMTVYAASKKFVTYFSKALELEV----AEHNIHVQLVMPNFVVTKMNAYTDRVMQGGLFFPNAYTFARSAVFTLGKTSETNGFWTHGIQ-YAIMKLAPLPIRTYLGHQLFKRLRIEALEQKQK 333          
BLAST of SMED30021475 vs. Ensembl Fly
Match: CG13284 (gene:FBgn0032614 transcript:FBtr0332334)

HSP 1 Score: 157.147 bits (396), Expect = 6.638e-45
Identity = 88/276 (31.88%), Postives = 153/276 (55.43%), Query Frame = -3
Query:   56 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTR----KFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 868
            +  +G W VVTG TDGIGK +  +LA++  NL+LISR +EKL+  + E+  ++++  K+I ADF K + +YD I + + G+ VG+L+NNVG  Y  P+  + D +S+    ++L  N  S  ++TR +L   + ++ G I+N+ S+S    +P +T+YA+ K  +  F+  L+ E+    A   I VQ   P +V++K++    R        P A  F  S +  L +  ++ G+ TH  + + + KL P  +   L  +   +LR +A  ++ K
Sbjct:   66 VDKFGQWAVVTGATDGIGKEYARELARQGINLVLISRTKEKLIAVTNEIESQYKVKTKWIAADFAKGREVYDQIEKELAGIDVGILVNNVGMMYEHPE--SLDLVSEDLLWNLLTVNMGSVTMLTRKILPQMIGRRKGAIVNLGSSSELQPLPNMTVYAASKKFVTYFSKALELEV----AEHNIHVQLVMPNFVVTKMNAYTDRVMQGGLFFPNAYTFARSAVFTLGKTSETNGFWTHGIQ-YAIMKLAPLPIRTYLGHQLFKRLRIEALEQKQK 334          
BLAST of SMED30021475 vs. Ensembl Zebrafish
Match: hsd17b12b (hydroxysteroid (17-beta) dehydrogenase 12b [Source:NCBI gene;Acc:322626])

HSP 1 Score: 182.185 bits (461), Expect = 1.163e-54
Identity = 97/253 (38.34%), Postives = 150/253 (59.29%), Query Frame = -3
Query:  116 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP-KFLNK 871
            +  S G W VVTG TDGIGK + E+LA+R F ++LISR +EKL   S+ +  K+++  K I+ADF    IY  I   + GL +G+L+NNVG +Y  P+F+      D    +M++ N +S   MTR++L   + +  GVI+N++SAS    +P+LT+Y+S K+ ++ F+  L AE  ++G    I +Q+  PF+V +KLS I+     +PT E +  + L  +    QS GY+ H+   W    L+P K LNK
Sbjct:   43 RASSLGKWAVVTGATDGIGKAYAEELARRGFAIVLISRTQEKLDEVSKAIESKYKVETKTISADFGSVDIYPKIESGLAGLEIGVLVNNVGVSYSYPEFFLNIPDVDSFINNMININIMSVCQMTRLVLPRMVDRSKGVILNVASASGMYPVPLLTLYSSTKAFVDFFSRGLDAEYKSKG----IIIQSVLPFYVTTKLSKIRKPTLDIPTPERYVKAQLSTIGLQTQSNGYLPHAIMGWVTASLLPAKLLNK 291          
BLAST of SMED30021475 vs. Ensembl Zebrafish
Match: hsd17b12a (hydroxysteroid (17-beta) dehydrogenase 12a [Source:NCBI gene;Acc:327417])

HSP 1 Score: 174.481 bits (441), Expect = 1.155e-51
Identity = 90/244 (36.89%), Postives = 143/244 (58.61%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSD-RDFI-DMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LA+R F+++LISR++EKL   ++ L   +++  K I  DF +  +Y  I + + GL +G+L+NNVG +Y  P+F+   H+ D  +FI  M++ N  S   MTR++L     +  GVI+N+SSAS    +P+LT+Y+S K+ ++ F+  LQ E   +G    I +Q+  PF+V +K++ I+      PT E +  + L  +    Q+ GY  H+   W    L P
Sbjct:   56 GKWAVVTGATDGIGKSYAEELARRGFSMMLISRSQEKLDDVAKSLESTYKVETKTIAVDFSQIDVYPKIEKGLAGLEIGILVNNVGISYSYPEFFL--HIPDLENFITTMINVNITSVCQMTRLVLPRMEARAKGVILNISSASGMFPVPLLTIYSSTKAFVDFFSRGLQTEYKCKG----IIIQSVLPFFVATKMTKIRKPTLDKPTPERYVAAELNTVGLQDQTNGYFPHAVMGWVTTILAP 293          
BLAST of SMED30021475 vs. Ensembl Zebrafish
Match: hsd17b3 (hydroxysteroid (17-beta) dehydrogenase 3 [Source:ZFIN;Acc:ZDB-GENE-040426-1339])

HSP 1 Score: 166.007 bits (419), Expect = 1.750e-48
Identity = 100/283 (35.34%), Postives = 160/283 (56.54%), Query Frame = -3
Query:   56 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVG---AAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 895
            P+ E+ F    S G W V+TG +DGIG+ + E+L+K+  ++I+ISRN+EKL   ++++       VK I ADF K  IY HI E++EGL +G+L+NNVG   +  P     T+D L +R + D+++CN  S V M RI+L     ++ GVI+N+SS  + +  P+ T+YA+ K  +  F+  LQAE  ++G    I +Q   PF V + ++G +    +  TAEEF  S L+ L    Q++G ITH+     L +++      VL   +      +  + RD+
Sbjct:   37 PLPEAFF---TSLGKWAVITGGSDGIGRAYAEELSKQGMSVIIISRNQEKLDRAAKKIELNTGGKVKVIAADFTKDDIYGHITENIEGLDIGVLVNNVGILPSQIPCKLLETSD-LEERIY-DIVNCNVKSMVKMCRIVLPGMQQRRRGVILNVSSGIAKIPCPIYTLYAASKVFVERFSQGLQAEYISKG----IIIQTVAPFGVSTAMTGHQKPDMVTFTAEEFVRSSLKYLKTGDQTYGSITHT----LLGRIVQSIPTWVLQSETFQHHFQEYVKNRDR 306          
BLAST of SMED30021475 vs. Ensembl Zebrafish
Match: hsd17b3 (hydroxysteroid (17-beta) dehydrogenase 3 [Source:ZFIN;Acc:ZDB-GENE-040426-1339])

HSP 1 Score: 165.236 bits (417), Expect = 3.350e-48
Identity = 99/283 (34.98%), Postives = 160/283 (56.54%), Query Frame = -3
Query:   56 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVG---AAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 895
            P+ E+ F    S G W V+TG +DGIG+ + E+L+K+  ++I+ISRN+EKL   ++++       VK I ADF K  IY HI E++EGL +G+L+NNVG   +  P     T+D L +R + D+++CN  S V M RI+L     ++ GVI+N+SS  + +  P+ T+YA+ K  +  F+  LQAE  ++G    + +Q   PF V + ++G +    +  TAEEF  S L+ L    Q++G ITH+     L +++      VL   +      +  + RD+
Sbjct:   37 PLPEAFF---TSLGKWAVITGGSDGIGRAYAEELSKQGMSVIIISRNQEKLDRAAKKIELNTGGKVKVIAADFTKDDIYGHITENIEGLDIGVLVNNVGILPSQIPCKLLETSD-LEERIY-DIVNCNVKSMVKMCRIVLPGMQQRRRGVILNVSSGIAKIPCPIYTLYAASKVFVERFSQGLQAEYISKG----VIIQTVAPFGVSTAMTGHQKPDMVTFTAEEFVRSSLKYLKTGDQTYGSITHT----LLGRIVQSIPTWVLQSETFQHHFQEYVKNRDR 306          
BLAST of SMED30021475 vs. Ensembl Zebrafish
Match: hsd20b2 (hydroxysteroid (20-beta) dehydrogenase 2 [Source:ZFIN;Acc:ZDB-GENE-030804-21])

HSP 1 Score: 150.214 bits (378), Expect = 3.586e-42
Identity = 89/273 (32.60%), Postives = 148/273 (54.21%), Query Frame = -3
Query:  122 YKLKPVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKT-KIYDHIAESVEGLPVGLLINNVGAAY----------PSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL 907
            +K+  +SE     +++YG W VVTG T GIG+ + E+LAKR  N++LISR+EEKL   ++E+ +K+      I ADF +   IY  I + +EGL +G+L+NNVG  Y          P P         D+    +L+CN LS   M R++L   + +  G+IIN+SS + +  +P++++Y++ K+ +  F+  L AE  ++G    I VQ   PF V + ++       LV +A  F    L  +     + G +TH+ ++  L+ + P +L
Sbjct:   37 FKVYVISEIWRTDLRTYGRWAVVTGATSGIGRAYAEELAKRGLNIVLISRSEEKLHRVAKEIEDKYNQKTHVIQADFTEGHSIYSTITKQLEGLEIGILVNNVGMNYIGVLANFLDVPDP---------DQRITQVLNCNTLSVTQMCRVILPGMVERGKGLIINISSEAGYQPVPMVSLYSATKAFVTYFSLGLNAEYRSKG----ITVQCVAPFMVSTNMTHNVPVNPLVKSAASFARDALNTVGYTTYTSGCLTHALQHIVLSIVFPGWL 296          
BLAST of SMED30021475 vs. Ensembl Xenopus
Match: hsd17b12 (hydroxysteroid (17-beta) dehydrogenase 12 [Source:NCBI gene;Acc:549988])

HSP 1 Score: 182.956 bits (463), Expect = 1.011e-54
Identity = 92/243 (37.86%), Postives = 146/243 (60.08%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRK-TKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LAKR  N++LISR+ EKL   ++++ EKF++  K I ADF K T+IY  I   +  L +G+L+NNVG +Y  P+++      +     M++ N  S   MTR++L   + +  GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQAE  ++G    + VQ+  PF+V +KL+ I+   +  P+ E +  S L  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELAKRGMNIVLISRSPEKLEEVAKQIKEKFKVETKIIAADFGKPTEIYGRIESGLRDLEIGVLVNNVGVSYEHPEYFLEIPDLENTLDKMININITSVCQMTRLVLPGMLGRGRGVILNISSASGMYPVPLLTVYSATKAFVDFFSRGLQAEYRSKG----VTVQSVLPFYVATKLAKIRKPTWDKPSPETYVQSALNTVGLQTQTNGYLPHAIMGWISTSLVP 294          
BLAST of SMED30021475 vs. Ensembl Xenopus
Match: hsd17b12 (hydroxysteroid (17-beta) dehydrogenase 12 [Source:NCBI gene;Acc:549988])

HSP 1 Score: 177.563 bits (449), Expect = 1.071e-52
Identity = 91/243 (37.45%), Postives = 145/243 (59.67%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRK-TKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LAKR  N++LISR+ EKL   ++++ EKF++  K I ADF K T+IY  I   +  L +G+L+NNVG +Y  P+++    + D + +   H        MTR++L   + +  GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQAE  ++G    + VQ+  PF+V +KL+ I+   +  P+ E +  S L  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELAKRGMNIVLISRSPEKLEEVAKQIKEKFKVETKIIAADFGKPTEIYGRIESGLRDLEIGVLVNNVGVSYEHPEYFL--EIPDLENVSTNHT-------MTRLVLPGMLGRGRGVILNISSASGMYPVPLLTVYSATKAFVDFFSRGLQAEYRSKG----VTVQSVLPFYVATKLAKIRKPTWDKPSPETYVQSALNTVGLQTQTNGYLPHAIMGWISTSLVP 285          
BLAST of SMED30021475 vs. Ensembl Xenopus
Match: hsd17b12 (hydroxysteroid (17-beta) dehydrogenase 12 [Source:NCBI gene;Acc:549988])

HSP 1 Score: 169.088 bits (427), Expect = 1.830e-49
Identity = 91/243 (37.45%), Postives = 146/243 (60.08%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRK-TKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LAKR  N++LISR+ EKL   ++++ EKF++  K I ADF K T+IY  I   +  L +G+L+NNVG +Y  P+++      +     M++ N +++V   RI+     L + GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQAE  ++G    + VQ+  PF+V +KL+ I+   +  P+ E +  S L  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELAKRGMNIVLISRSPEKLEEVAKQIKEKFKVETKIIAADFGKPTEIYGRIESGLRDLEIGVLVNNVGVSYEHPEYFLEIPDLENTLDKMININ-ITSVCQVRIV---GFLGR-GVILNISSASGMYPVPLLTVYSATKAFVDFFSRGLQAEYRSKG----VTVQSVLPFYVATKLAKIRKPTWDKPSPETYVQSALNTVGLQTQTNGYLPHAIMGWISTSLVP 289          
BLAST of SMED30021475 vs. Ensembl Xenopus
Match: hsd17b12 (hydroxysteroid (17-beta) dehydrogenase 12 [Source:NCBI gene;Acc:549988])

HSP 1 Score: 166.007 bits (419), Expect = 2.631e-48
Identity = 88/243 (36.21%), Postives = 138/243 (56.79%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRK-TKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LAKR  N++LISR+ EKL   ++++ EKF++  K I ADF K T+IY  I   +  L +G+L+NNVG +Y  P+++             L    L  V     + +  +    GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQAE  ++G    + VQ+  PF+V +KL+ I+   +  P+ E +  S L  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELAKRGMNIVLISRSPEKLEEVAKQIKEKFKVETKIIAADFGKPTEIYGRIESGLRDLEIGVLVNNVGVSYEHPEYF-------------LEIPDLENVSTNHTVQQLPLTDPVGVILNISSASGMYPVPLLTVYSATKAFVDFFSRGLQAEYRSKG----VTVQSVLPFYVATKLAKIRKPTWDKPSPETYVQSALNTVGLQTQTNGYLPHAIMGWISTSLVP 281          
BLAST of SMED30021475 vs. Ensembl Xenopus
Match: hsd17b12 (hydroxysteroid (17-beta) dehydrogenase 12 [Source:NCBI gene;Acc:549988])

HSP 1 Score: 161.384 bits (407), Expect = 1.223e-46
Identity = 86/243 (35.39%), Postives = 133/243 (54.73%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRK-TKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LAKR  N++LISR+ EKL   ++++ EKF++  K I ADF K T+IY  I   +  L +G+L+NNVG +Y  P+++      +     M++ N  S   MTR++L   + +  GVI+N+SSAS    +P+LT+Y++ KS+L                          PF+V +KL+ I+   +  P+ E +  S L  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELAKRGMNIVLISRSPEKLEEVAKQIKEKFKVETKIIAADFGKPTEIYGRIESGLRDLEIGVLVNNVGVSYEHPEYFLEIPDLENTLDKMININITSVCQMTRLVLPGMLGRGRGVILNISSASGMYPVPLLTVYSATKSVL--------------------------PFYVATKLAKIRKPTWDKPSPETYVQSALNTVGLQTQTNGYLPHAIMGWISTSLVP 272          
BLAST of SMED30021475 vs. Ensembl Mouse
Match: Hsd17b12 (hydroxysteroid (17-beta) dehydrogenase 12 [Source:MGI Symbol;Acc:MGI:1926967])

HSP 1 Score: 183.341 bits (464), Expect = 4.017e-55
Identity = 95/267 (35.58%), Postives = 157/267 (58.80%), Query Frame = -3
Query:   56 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 856
            G W VVTG TDGIGK + E+LAKR   ++LISR+++KL   S  + EKF +  + I  DF    IYD I   + GL +G+L+NNVG +Y  P+++      D     +++ N LS   +TR++L   + +  GVI+N+SSAS  L +P+LT+Y++ K+ ++ F+ CL  E  ++G    IFVQ+  P+ V +KL+ I+      P+AE F  S ++ +    ++ GY+ HS     +N ++P+++   ++      LR++  +KR K
Sbjct:   50 GEWAVVTGGTDGIGKAYAEELAKRGMKIVLISRSQDKLNQVSNNIKEKFNVETRTIAVDFSLDDIYDKIKTGLSGLEIGVLVNNVGMSYEYPEYFLEIPDLDNTIKKLININVLSVCKVTRLVLPGMVERSKGVILNISSASGMLPVPLLTIYSATKAFVDFFSQCLHEEYKSKG----IFVQSVMPYLVATKLAKIQKPTLDKPSAETFVKSAIKTVGLQTRTTGYVIHSLMG-SINSIMPRWMYFKIIMGFSKSLRNRYLKKRKK 311          
BLAST of SMED30021475 vs. Ensembl Mouse
Match: Hsdl1 (hydroxysteroid dehydrogenase like 1 [Source:MGI Symbol;Acc:MGI:1919802])

HSP 1 Score: 153.295 bits (386), Expect = 1.401e-43
Identity = 91/279 (32.62%), Postives = 151/279 (54.12%), Query Frame = -3
Query:   89 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADF-RKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKF------LVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL---NKVLVRRSMMK 895
            P   S+ D IK YG W V++G TDGIGK + E+LA    N+ILIS+ EEKL   ++ + + +R+    + ADF R  +IY  I E++    +G+L+N+VGA YP P +++   + +    D+++ N  +  LM  I+L   + ++ G I+ +SS S     P L  +++ K+ L+ F+  LQ E  ++G    IFVQ+  PF+V S  SG     F      L P+   +    +  L  + ++ GY +HS +  F  + +P++L      L+ RS+ K
Sbjct:   33 PRLGSRPDLIKQYGRWAVISGATDGIGKAYAEELASHGLNVILISQEEEKLQAAAKHIADTYRVETLVLVADFSRGREIYAPIREALRDRDIGILVNDVGAFYPYPQYFS--QVPEDTLWDIVNVNIAAASLMVHIVLPGMVERKKGAIVTVSSGSCCKPTPQLAAFSASKAYLDHFSRALQYEYASKG----IFVQSLIPFYVTS--SGAAPASFLHRCPWLAPSPRVYAQHAVSTLGISKRTTGYWSHSIQFLF-AQYMPEWLWVWGANLLNRSLRK 302          
BLAST of SMED30021475 vs. Ensembl Mouse
Match: Hsdl1 (hydroxysteroid dehydrogenase like 1 [Source:MGI Symbol;Acc:MGI:1919802])

HSP 1 Score: 152.525 bits (384), Expect = 4.519e-43
Identity = 91/279 (32.62%), Postives = 151/279 (54.12%), Query Frame = -3
Query:   89 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADF-RKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKF------LVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL---NKVLVRRSMMK 895
            P   S+ D IK YG W V++G TDGIGK + E+LA    N+ILIS+ EEKL   ++ + + +R+    + ADF R  +IY  I E++    +G+L+N+VGA YP P +++   + +    D+++ N  +  LM  I+L   + ++ G I+ +SS S     P L  +++ K+ L+ F+  LQ E  ++G    IFVQ+  PF+V S  SG     F      L P+   +    +  L  + ++ GY +HS +  F  + +P++L      L+ RS+ K
Sbjct:   54 PRLGSRPDLIKQYGRWAVISGATDGIGKAYAEELASHGLNVILISQEEEKLQAAAKHIADTYRVETLVLVADFSRGREIYAPIREALRDRDIGILVNDVGAFYPYPQYFS--QVPEDTLWDIVNVNIAAASLMVHIVLPGMVERKKGAIVTVSSGSCCKPTPQLAAFSASKAYLDHFSRALQYEYASKG----IFVQSLIPFYVTS--SGAAPASFLHRCPWLAPSPRVYAQHAVSTLGISKRTTGYWSHSIQFLF-AQYMPEWLWVWGANLLNRSLRK 323          
BLAST of SMED30021475 vs. Ensembl Mouse
Match: Hsd17b3 (hydroxysteroid (17-beta) dehydrogenase 3 [Source:MGI Symbol;Acc:MGI:107177])

HSP 1 Score: 147.902 bits (372), Expect = 1.475e-41
Identity = 83/260 (31.92%), Postives = 143/260 (55.00%), Query Frame = -3
Query:   92 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDR-DFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMM 868
            ++S G W V+TG  DGIGK +  +LA+   N++LISR  EKL   ++E+       VK + ADF +  IYDHI E +EGL +G+L+NNVG     P F+ +  LS   +  +++HCN  S V MT+++LK    ++ G+I+N+SS ++    P+ ++Y++ K+ +  F+  L  E   +G    I +Q  TP+ + + ++     K +  TA+EF    L+ +    +S G + H      LN++  +       +R ++
Sbjct:   40 LRSMGQWAVITGAGDGIGKAYSFELARHGLNVVLISRTLEKLQTIAEEIERTTGSCVKIVQADFTREDIYDHIKEHLEGLEIGILVNNVGML---PSFFPSHFLSTSGESQNLIHCNITSVVKMTQLVLKHMESRRKGLILNISSGAALRPWPLYSLYSASKAFVYTFSKALSVEYRDKG----IIIQVLTPYSISTPMTKYLNNK-MTKTADEFVKESLKYVTIGAESCGCLAHEIIAIILNRIPSRIFYSSTAQRFLL 291          
BLAST of SMED30021475 vs. Ensembl Mouse
Match: Hsd17b3 (hydroxysteroid (17-beta) dehydrogenase 3 [Source:MGI Symbol;Acc:MGI:107177])

HSP 1 Score: 147.902 bits (372), Expect = 1.475e-41
Identity = 83/260 (31.92%), Postives = 143/260 (55.00%), Query Frame = -3
Query:   92 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDR-DFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMM 868
            ++S G W V+TG  DGIGK +  +LA+   N++LISR  EKL   ++E+       VK + ADF +  IYDHI E +EGL +G+L+NNVG     P F+ +  LS   +  +++HCN  S V MT+++LK    ++ G+I+N+SS ++    P+ ++Y++ K+ +  F+  L  E   +G    I +Q  TP+ + + ++     K +  TA+EF    L+ +    +S G + H      LN++  +       +R ++
Sbjct:   40 LRSMGQWAVITGAGDGIGKAYSFELARHGLNVVLISRTLEKLQTIAEEIERTTGSCVKIVQADFTREDIYDHIKEHLEGLEIGILVNNVGML---PSFFPSHFLSTSGESQNLIHCNITSVVKMTQLVLKHMESRRKGLILNISSGAALRPWPLYSLYSASKAFVYTFSKALSVEYRDKG----IIIQVLTPYSISTPMTKYLNNK-MTKTADEFVKESLKYVTIGAESCGCLAHEIIAIILNRIPSRIFYSSTAQRFLL 291          
BLAST of SMED30021475 vs. UniProt/SwissProt
Match: sp|O70503|DHB12_MOUSE (Very-long-chain 3-oxoacyl-CoA reductase OS=Mus musculus OX=10090 GN=Hsd17b12 PE=1 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 2.810e-54
Identity = 95/267 (35.58%), Postives = 157/267 (58.80%), Query Frame = -3
Query:   56 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 856
            G W VVTG TDGIGK + E+LAKR   ++LISR+++KL   S  + EKF +  + I  DF    IYD I   + GL +G+L+NNVG +Y  P+++      D     +++ N LS   +TR++L   + +  GVI+N+SSAS  L +P+LT+Y++ K+ ++ F+ CL  E  ++G    IFVQ+  P+ V +KL+ I+      P+AE F  S ++ +    ++ GY+ HS     +N ++P+++   ++      LR++  +KR K
Sbjct:   50 GEWAVVTGGTDGIGKAYAEELAKRGMKIVLISRSQDKLNQVSNNIKEKFNVETRTIAVDFSLDDIYDKIKTGLSGLEIGVLVNNVGMSYEYPEYFLEIPDLDNTIKKLININVLSVCKVTRLVLPGMVERSKGVILNISSASGMLPVPLLTIYSATKAFVDFFSQCLHEEYKSKG----IFVQSVMPYLVATKLAKIQKPTLDKPSAETFVKSAIKTVGLQTRTTGYVIHSLMG-SINSIMPRWMYFKIIMGFSKSLRNRYLKKRKK 311          
BLAST of SMED30021475 vs. UniProt/SwissProt
Match: sp|Q28IU1|DHB12_XENTR (Very-long-chain 3-oxoacyl-CoA reductase OS=Xenopus tropicalis OX=8364 GN=hsd17b12 PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 5.356e-54
Identity = 92/243 (37.86%), Postives = 146/243 (60.08%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRK-TKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LAKR  N++LISR+ EKL   ++++ EKF++  K I ADF K T+IY  I   +  L +G+L+NNVG +Y  P+++      +     M++ N  S   MTR++L   + +  GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQAE  ++G    + VQ+  PF+V +KL+ I+   +  P+ E +  S L  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELAKRGMNIVLISRSPEKLEEVAKQIKEKFKVETKIIAADFGKPTEIYGRIESGLRDLEIGVLVNNVGVSYEHPEYFLEIPDLENTLDKMININITSVCQMTRLVLPGMLGRGRGVILNISSASGMYPVPLLTVYSATKAFVDFFSRGLQAEYRSKG----VTVQSVLPFYVATKLAKIRKPTWDKPSPETYVQSALNTVGLQTQTNGYLPHAIMGWISTSLVP 294          
BLAST of SMED30021475 vs. UniProt/SwissProt
Match: sp|Q6QA33|DH12B_DANRE (Very-long-chain 3-oxoacyl-CoA reductase-B OS=Danio rerio OX=7955 GN=hsd17b12b PE=2 SV=2)

HSP 1 Score: 182.185 bits (461), Expect = 8.778e-54
Identity = 97/253 (38.34%), Postives = 150/253 (59.29%), Query Frame = -3
Query:  116 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP-KFLNK 871
            +  S G W VVTG TDGIGK + E+LA+R F ++LISR +EKL   S+ +  K+++  K I+ADF    IY  I   + GL +G+L+NNVG +Y  P+F+      D    +M++ N +S   MTR++L   + +  GVI+N++SAS    +P+LT+Y+S K+ ++ F+  L AE  ++G    I +Q+  PF+V +KLS I+     +PT E +  + L  +    QS GY+ H+   W    L+P K LNK
Sbjct:   43 RASSLGKWAVVTGATDGIGKAYAEELARRGFAIVLISRTQEKLDEVSKAIESKYKVETKTISADFGSVDIYPKIESGLAGLEIGVLVNNVGVSYSYPEFFLNIPDVDSFINNMININIMSVCQMTRLVLPRMVDRSKGVILNVASASGMYPVPLLTLYSSTKAFVDFFSRGLDAEYKSKG----IIIQSVLPFYVTTKLSKIRKPTLDIPTPERYVKAQLSTIGLQTQSNGYLPHAIMGWVTASLLPAKLLNK 291          
BLAST of SMED30021475 vs. UniProt/SwissProt
Match: sp|Q6P7R8|DHB12_RAT (Very-long-chain 3-oxoacyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Hsd17b12 PE=2 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 3.862e-53
Identity = 90/245 (36.73%), Postives = 148/245 (60.41%), Query Frame = -3
Query:  122 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL 856
            G W VVTG TDGIGK + E+LAKR   ++LISR+++KL   S  + EKF +  + I  DF    IYD I   + GL +G+L+NNVG +Y  P+++      D     +++ N LS   +TR++L   + +  GVI+N+SSAS  L +P+LT+Y++ K+ ++ F+ CL  E  ++G    IFVQ+  PF+V +KL+ I+      P+AE F  S ++ +    ++ GY+ H+     +N ++P+++
Sbjct:   50 GEWAVVTGGTDGIGKSYAEELAKRGMKIVLISRSQDKLKEVSNNIKEKFNVETRTIAVDFSLDDIYDKIKTGLSGLEIGVLVNNVGMSYEYPEYFLEIPDLDNTIKKLININVLSICKVTRLVLPGMVERSKGVILNISSASGMLPVPLLTVYSATKAFVDFFSQCLHEEYKSKG----IFVQSVLPFFVATKLAKIRKPTLDKPSAETFVKSAIKTVGLQTRTTGYVIHAIMG-SINSILPRWI 289          
BLAST of SMED30021475 vs. UniProt/SwissProt
Match: sp|Q4R5G7|DHB12_MACFA (Very-long-chain 3-oxoacyl-CoA reductase OS=Macaca fascicularis OX=9541 GN=HSD17B12 PE=2 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.320e-52
Identity = 88/240 (36.67%), Postives = 141/240 (58.75%), Query Frame = -3
Query:  137 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKL 856
            G W VVTG TDGIGK + E+LAKR   ++LISR+++KL   S E+ EKF++  + I  DF    IYD I   + GL +G+L+NNVG +Y  P+++      D     M++ N LS   MT+++L   + +  G I+N+SS S    +P+LT+Y++ K+ ++ F+ CL  E  ++G    IFVQ+  P++V +KL+ I+      PT E F  S ++ +    ++ GY+ H    W ++ L
Sbjct:   50 GEWAVVTGGTDGIGKSYAEELAKRGMKVVLISRSQDKLDQVSSEIKEKFKVETRTIAVDFTLEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMFPVPLLTIYSATKTFVDFFSQCLHEEYRSKG----IFVQSVLPYFVATKLAKIRKPTLDKPTPETFVKSAIKTVGLQSRTNGYLIHVLMGWIISNL 285          
BLAST of SMED30021475 vs. TrEMBL
Match: A0A3M7SUC4 (Very-long-chain 3-oxoacyl-reductase OS=Brachionus plicatilis OX=10195 GN=BpHYR1_039333 PE=3 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 2.946e-59
Identity = 104/274 (37.96%), Postives = 165/274 (60.22%), Query Frame = -3
Query:   50 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADF-RKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIK 868
            +K  G+W VVTG TDGIGK + EKLA    NL+LISR E+KL  Q++E+ EKF++  K I ADF  +  IY  I + + GL +G+L+NNVG +YP P+F+ A   +++   DM++CN  S   MT I+L + + K+ G+IIN +S S  +  P+LT+Y++ K+ ++ F+  L  E  ++G    I VQ+  P++V +KLS ++ R F+ PT  EF  S L  +     + G + H+ + W    ++P  L   +    M+ +R +A RK  K++
Sbjct:   47 LKKIGSWAVVTGCTDGIGKAYAEKLANLGLNLVLISRTEQKLKNQAKEIEEKFKVDTKIIAADFCEQNSIYSEIKQQLTGLDIGILVNNVGVSYPYPEFFDAFAKNEKAVSDMINCNITSVTKMTAIVLPTMIEKRKGLIINNASGSGRIPTPLLTVYSASKAYVDFFSRALNTEYKSKG----IVVQSLCPYFVSTKLSALR-RNFMTPTPAEFVSSALATVNSQPVTNGCLIHNLQGWLYEDVVPSALVDKMTFNMMLGVRAKAIRKAKKLE 315          
BLAST of SMED30021475 vs. TrEMBL
Match: C3Y616 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_267231 PE=3 SV=1)

HSP 1 Score: 200.29 bits (508), Expect = 2.367e-58
Identity = 106/273 (38.83%), Postives = 163/273 (59.71%), Query Frame = -3
Query:   50 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIK 868
            IK+ G+W VVTG+TDGIGK + E+LA R  N++LISR E+KL   + E+  K  +  K + ADF    IYD+I+  +EGL +  L+NNVG  Y  PDFY A+  +D D + ML+ N LS V MTRI+L   + ++ GVI+N+SSAS  +  P+L++Y++ K+ ++ F+ CL  E  ++G    I +Q+ TP +V +KLSGI+      P+   +  S L  +     +FGY TH+   W  + L+P+ L   +     + +R  A +K  + K
Sbjct:   40 IKNCGSWAVVTGSTDGIGKAYAEELAARGLNIVLISRTEDKLKAVAAEIEGKAGVQTKIVVADFSSADIYDNISLQLEGLDIARLVNNVGVGYKFPDFY-ANTPADMDEM-MLNVNSLSVVKMTRIVLPGMVERKKGVILNISSASGVIPTPLLSLYSASKAFVDYFSRCLAQEYRSKG----IIIQSVTPNFVATKLSGIRKTSLFAPSPTSYVRSALNTVGLADHTFGYSTHALGGWLFD-LLPQRLYMSVSMSIFLGVRKAALKKLQRKK 305          
BLAST of SMED30021475 vs. TrEMBL
Match: A0A3Q3KAP3 (Hydroxysteroid (17-beta) dehydrogenase 12a OS=Monopterus albus OX=43700 PE=3 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 2.723e-57
Identity = 106/272 (38.97%), Postives = 160/272 (58.82%), Query Frame = -3
Query:   77 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSD-RDFI-DMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPK----FLNKVLVRRSMM------KLRDQ 856
            G W VVTG TDGIGK + E+LA+R F ++LISR+++KL   ++ L E++++  K I  DF KT IY  I E + GL +G+L+NNVG +YP P++Y   H+ D  DFI +M++ N  S   MTR++L   + +  GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQ E   QG    I +Q+  PF+V +K++ IK      PT E +  + L  +    Q+ GY  H+   W   KL+P     +L   + RR         KLRDQ
Sbjct:   56 GKWAVVTGATDGIGKSYAEELARRGFAMVLISRSQDKLDDVAKSLKEQYKVETKTIAVDFGKTDIYSKINEGLAGLEIGVLVNNVGVSYPYPEYYL--HIPDLDDFITNMINVNMTSVCQMTRLVLPRMVERSKGVILNISSASGMYPLPLLTVYSATKAFVDFFSRGLQEEYRRQG----IIIQSVLPFYVATKMTRIKKPTLDKPTPERYVAAELNTVGLQSQTNGYFPHAVMGWVTTKLVPSSIVIYLGAGMNRRQRTGYLQRRKLRDQ 321          
BLAST of SMED30021475 vs. TrEMBL
Match: A0A3P8QX52 (Hydroxysteroid (17-beta) dehydrogenase 12a OS=Astatotilapia calliptera OX=8154 PE=3 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 4.634e-56
Identity = 102/271 (37.64%), Postives = 163/271 (60.15%), Query Frame = -3
Query:   50 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRD-FI-DMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIK 856
            G W VVTG TDGIGK + E+LA+R F ++LISR++EKL   ++ + E+F++  + I  DF KT IY  I E + GL +G+L+NNVG +YP P++Y   H+ D D FI +M++ N  S   MTR++L     +  GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQ E   QG    I +Q+  PF+V++K++ IK      PT E +  + L  +    Q+ GY  H+   W   KL+P  +  + +  +M +L+     +R K++
Sbjct:   53 GKWAVVTGATDGIGKSYAEELARRGFAMMLISRSQEKLDDVARSIEEQFKVETRTIAVDFGKTDIYSKIEEGLAGLEIGVLVNNVGVSYPYPEYYL--HIPDLDNFITNMINVNMTSVCQMTRLVLPRMCERSKGVILNISSASGMYPLPLLTVYSATKAFVDFFSRGLQEEYRRQG----ILIQSVLPFFVVTKMTRIKKPTLDKPTPERYVAAELTTVGLQSQTNGYFPHAVMGWVTTKLVPTSI-VIFLGATMNRLQRTGYLQRRKLR 316          
BLAST of SMED30021475 vs. TrEMBL
Match: A0A3B4FSS6 (Hydroxysteroid (17-beta) dehydrogenase 12a OS=Pundamilia nyererei OX=303518 PE=3 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 4.634e-56
Identity = 102/271 (37.64%), Postives = 163/271 (60.15%), Query Frame = -3
Query:   50 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRD-FI-DMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIK 856
            G W VVTG TDGIGK + E+LA+R F ++LISR++EKL   ++ + E+F++  + I  DF KT IY  I E + GL +G+L+NNVG +YP P++Y   H+ D D FI +M++ N  S   MTR++L     +  GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQ E   QG    I +Q+  PF+V++K++ IK      PT E +  + L  +    Q+ GY  H+   W   KL+P  +  + +  +M +L+     +R K++
Sbjct:   53 GKWAVVTGATDGIGKSYAEELARRGFAMMLISRSQEKLDDVARSIEEQFKVETRTIAVDFGKTDIYSKIEEGLAGLEIGVLVNNVGVSYPYPEYYL--HIPDLDNFITNMINVNMTSVCQMTRLVLPRMCERSKGVILNISSASGMYPLPLLTVYSATKAFVDFFSRGLQEEYRRQG----ILIQSVLPFFVVTKMTRIKKPTLDKPTPERYVAAELTTVGLQSQTNGYFPHAVMGWVTTKLVPTSI-VIFLGATMNRLQRTGYLQRRKLR 316          
BLAST of SMED30021475 vs. Ensembl Cavefish
Match: hsd17b12b (very-long-chain 3-oxoacyl-CoA reductase-B-like [Source:NCBI gene;Acc:103024613])

HSP 1 Score: 186.422 bits (472), Expect = 2.151e-56
Identity = 96/267 (35.96%), Postives = 154/267 (57.68%), Query Frame = -3
Query:   56 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDK 856
            G W VVTG TDGIGK + E+LA+R F+++LISR +EKL   S+ +  K+++  K I+ADF    IY  I   +  L +G+L+NNVG +Y  P+F+      D     M++ N  S   MTR++L   + +  GVI+N+SSAS    +P+LT+Y+S K+ ++ F+  L AE  ++G    + +Q+  PF+V +KLS IK     +PT E +  S +  +   +Q+ GY+ H+   W    L+P  L   LV +  +  R +  +K+ +
Sbjct:   51 GAWAVVTGATDGIGKAYAEELARRGFSIVLISRTQEKLDEVSKAIESKYKVQTKTISADFGCVDIYPKIQAGLANLQIGILVNNVGVSYSYPEFFLNVPNLDNVISSMININISSVCQMTRLVLPGMVERSKGVILNISSASGMYPVPLLTLYSSSKAFVDFFSRALDAEYKSKG----VIIQSVLPFYVTTKLSKIKRASLDIPTPERYVRSQISTVGLQNQTNGYLPHAVMGWVTTALLPARLVSNLVMKMGLSQRARYLKKQKQ 313          
BLAST of SMED30021475 vs. Ensembl Cavefish
Match: hsd17b12a (hydroxysteroid 17-beta dehydrogenase 12 [Source:NCBI gene;Acc:103027081])

HSP 1 Score: 186.422 bits (472), Expect = 2.533e-56
Identity = 93/244 (38.11%), Postives = 148/244 (60.66%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRD-FID-MLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LA+R F+++LISR +EKL   +Q L  ++++  K +  DF +  IY  I  ++ GL +G+L+NNVG +YP P+F+   H+ D D FI+ M++ N  S   MTR++L     +  GVI+N+SSAS    +P+LT+Y+S K+ ++ F+  LQAE  ++G    I +Q+  PF+V +K++ I+      PT + +  + L  +    Q+ GY  H+   W    L+P
Sbjct:   48 GKWAVVTGATDGIGKSYAEELARRGFSMMLISRTQEKLDDVAQSLERRYKVETKTVAVDFGQVDIYPKIETALSGLEIGVLVNNVGISYPYPEFFL--HIPDLDNFINTMINVNITSVCQMTRLVLPRMEARAKGVILNISSASGMYPVPLLTVYSSTKAFVDFFSRGLQAEYKSKG----IIIQSVLPFFVATKMTKIRKPTLDKPTPDRYVAAELTTVGLQDQTNGYFPHAVMGWLTTVLVP 285          
BLAST of SMED30021475 vs. Ensembl Cavefish
Match: hsd17b12a (hydroxysteroid 17-beta dehydrogenase 12 [Source:NCBI gene;Acc:103027081])

HSP 1 Score: 172.555 bits (436), Expect = 4.831e-51
Identity = 89/243 (36.63%), Postives = 144/243 (59.26%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRD-FIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LA+R F+++LISR +EKL   +Q L  ++++  K +  DF +  IY  I  ++ GL +G+L+NNVG +YP P+F+   H+ D D FI+ +    +++V   + L      K  GVI+N+SSAS    +P+LT+Y+S K+ ++ F+  LQAE  ++G    I +Q+  PF+V +K++ I+      PT + +  + L  +    Q+ GY  H+   W    L+P
Sbjct:   48 GKWAVVTGATDGIGKSYAEELARRGFSMMLISRTQEKLDDVAQSLERRYKVETKTVAVDFGQVDIYPKIETALSGLEIGVLVNNVGISYPYPEFFL--HIPDLDNFINTMINVNITSVCQFKPLFPHFRAK--GVILNISSASGMYPVPLLTVYSSTKAFVDFFSRGLQAEYKSKG----IIIQSVLPFFVATKMTKIRKPTLDKPTPDRYVAAELTTVGLQDQTNGYFPHAVMGWLTTVLVP 282          
BLAST of SMED30021475 vs. Ensembl Cavefish
Match: hsd17b3 (hydroxysteroid 17-beta dehydrogenase 3 [Source:NCBI gene;Acc:103031519])

HSP 1 Score: 149.058 bits (375), Expect = 4.603e-42
Identity = 86/256 (33.59%), Postives = 140/256 (54.69%), Query Frame = -3
Query:  137 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVG---AAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKL 895
            P+ E+ F    S G W V+TG +DGIGK + ++LA+R  N+++ISR +EKL   ++E+  K    V+ I ADF K  IY  I E++EGL +GLL+NNVG      P     TA+   +     +++CN  + V M  I+L   + +  G+++N+SS  + +  P   +YA+ K+ +  F+  L AE  + G    I +Q+ +PF V + ++G      +  TAEEF  + L  L    Q++G + HS   W +  +
Sbjct:   37 PLPENFFT---SKGKWAVITGGSDGIGKAYAQELARRGLNVVIISRTKEKLDGVAREIELKTEAKVRAIAADFTKDDIYRDIEENIEGLDIGLLVNNVGILPTQLPCILLETAN--LEEKINQVINCNVKTMVKMCHIVLPGMVERGRGIVLNVSSGIAKIPCPTYALYAASKAFVERFSQGLLAEYRSSG----ILIQSVSPFGVSTTMTGHPKPDLITFTAEEFVRNSLMYLKTGEQTYGSVRHSILGWVIQAI 283          
BLAST of SMED30021475 vs. Ensembl Cavefish
Match: hsd20b2 (very-long-chain 3-oxoacyl-CoA reductase-like [Source:NCBI gene;Acc:103033448])

HSP 1 Score: 149.443 bits (376), Expect = 5.527e-42
Identity = 86/264 (32.58%), Postives = 148/264 (56.06%), Query Frame = -3
Query:  122 YKLKPVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTAD-HLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL 907
            +K+  +SE     ++ +G W VVTG T GIG+ +  +LA+R  +++LISR+EEKLL  ++E+  ++R   + I ADF   + IY  IA+ ++GL +G+L+NNV   Y     +  D   S++    +++CN LS   MTR++L   + +  G+IIN+SS ++    P+L +Y++ K  +  F+ CL +E  ++G    I VQ   PF V + ++       LV +AE F    L  +  +  + G +TH+ +N  L+   P + 
Sbjct:   36 FKVYMLSEVWRTDVRKFGQWAVVTGATSGIGRAYALELARRGLDIVLISRSEEKLLQTAKEIESQYRRQTRIIQADFTGGESIYPAIAQQLKGLEIGILVNNVAMNYAEEFAHFLDVPDSEQRITQVINCNILSVTQMTRVILPHMVERGSGLIINLSSEAASKPQPMLALYSATKIFVTYFSRCLHSEYRSRG----ITVQCVAPFVVSTNMTNNVPVSPLVKSAESFARDALNTVGYSSFTSGCLTHALQNIALSIFFPAWF 295          
BLAST of SMED30021475 vs. Ensembl Sea Lamprey
Match: hsd17b12b (hydroxysteroid (17-beta) dehydrogenase 12b [Source:NCBI gene;Acc:322626])

HSP 1 Score: 155.221 bits (391), Expect = 1.241e-45
Identity = 86/262 (32.82%), Postives = 150/262 (57.25%), Query Frame = -3
Query:   62 VVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKR 844
            VVTG TDGIGK + E++ K    L L+  +        +E+ E+F++  K +  DF  ++ IY  +  ++  LPVG+L+NNVG +Y  P+++      D    ++L+ N LS + MT+I+L   + ++GGVIIN+SS S+   +P+L++Y++ K  ++ F+  L  E  ++G    I VQ+  PF+V S +S I+     +P AE F  S L  +    +++GY+ H+ + W L+ ++P      L  ++++KL +Q  R R
Sbjct:    2 VVTGATDGIGKAYVEQMLKTDLPLRLVVCSSHNCAQIVREMSERFKVETKTVAVDFTASEEIYPKVLAALRDLPVGVLVNNVGISYTYPEYFLDIPNLDDMIKNILNVNVLSVLKMTQIILPGMLERKGGVIINISSGSALFPVPLLSLYSATKVFVDFFSRGLNTEYKSKG----IIVQSVLPFYVKSNMSRIQRATLNMPEAETFACSALATVGRQSRTYGYLPHALQAW-LSNMVP----TCLAMKTILKL-NQGIRAR 253          
BLAST of SMED30021475 vs. Ensembl Sea Lamprey
Match: hsd17b12b (hydroxysteroid (17-beta) dehydrogenase 12b [Source:NCBI gene;Acc:322626])

HSP 1 Score: 150.214 bits (378), Expect = 9.458e-44
Identity = 84/263 (31.94%), Postives = 152/263 (57.79%), Query Frame = -3
Query:   62 VVTGTTDGIGKCFCEKLAKRKFNLILISRN-EEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKR 844
            VVTG TDGIGK + E+++ R   +   +++   +L +    + E+F++  K +  DF  ++ IY  +  ++  LPVG+L+NNVG +Y  P+++      D    ++L+ N LS + MT+I+L   + ++GGVIIN+SS S+   +P+L++Y++ K  ++ F+  L  E  ++G    I VQ+  PF+V S +S I+     +P AE F  S L  +    +++GY+ H+ + W L+ ++P      L  ++++KL +Q  R R
Sbjct:    2 VVTGATDGIGKAYVEQVSSRGRIMSPTAKSLPTRLGVAHVYVCERFKVETKTVAVDFTASEEIYPKVLAALRDLPVGVLVNNVGISYTYPEYFLDIPNLDDMIKNILNVNVLSVLKMTQIILPGMLERKGGVIINISSGSALFPVPLLSLYSATKVFVDFFSRGLNTEYKSKG----IIVQSVLPFYVKSNMSRIQRATLNMPEAETFACSALATVGRQSRTYGYLPHALQAW-LSNMVP----TCLAMKTILKL-NQGIRAR 254          
BLAST of SMED30021475 vs. Ensembl Sea Lamprey
Match: dhrs7b (dehydrogenase/reductase (SDR family) member 7B [Source:NCBI gene;Acc:550454])

HSP 1 Score: 53.5286 bits (127), Expect = 2.288e-8
Identity = 53/208 (25.48%), Postives = 87/208 (41.83%), Query Frame = -3
Query:  272 YGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRI---------SVKYITADFRKTKIYDHIAESVEGL--PVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQG-GVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLS 859
            +G  VVVTG + G+G             L+L  R+  +L     EL E+ RI         + + +  D          AE V      V +L++  G +Y      TA  +  +    ++  N    + +   LL S MLKQG G I+ +SS    + IP  + YA+ K     + + L+AE+   G    + V   +P ++ + LS
Sbjct:   54 HGKVVVVTGASSGLGLACARAFHAEGARLVLCGRDLRRLESLRDELSERVRILAPQGPEDRTPRVVPFDLGSPGEVARAAELVVAAHGRVDVLVSGAGISYRGEAALTAMDVHRQ----LMEVNYFGAIALLTALLPS-MLKQGEGHIVAISSVQGRIAIPFRSAYAASKHATQAYFDSLRAEVADSG----LKVTVVSPGYIRTNLS 252          
BLAST of SMED30021475 vs. Ensembl Sea Lamprey
Match: kdsr (3-ketodihydrosphingosine reductase [Source:ZFIN;Acc:ZDB-GENE-040426-853])

HSP 1 Score: 49.2914 bits (116), Expect = 4.225e-7
Identity = 43/174 (24.71%), Postives = 78/174 (44.83%), Query Frame = -3
Query:  344 VTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFR--------ISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEI 841
            VTG + GIGK    +  ++   + L++RNE KLL   +E  E           ISV  ++ DF + +     A+   G PV +L+N  G +         + +  ++F  ++  N L +V  +  ++     ++ G ++ +SS +  L +   T Y+  K  L      LQ E+
Sbjct:    4 VTGGSSGIGKAIAIECVRQGACVTLVARNEAKLLQAKREAEEHVVNESQAVLCISVD-VSNDFNQVESVIKQAQEKLG-PVDMLVNAAGTSVAG----RFEDIKPQEFKRLMEVNYLGSVFTSLAVVPGMKERRKGRVVYVSSQAGQLGLYGFTAYSPTKFALRGLAEALQMEV 171          
BLAST of SMED30021475 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010315.1 (pep scaffold:Pmarinus_7.0:GL479994:4152:14615:1 gene:ENSPMAG00000009341.1 transcript:ENSPMAT00000010315.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 6.242e-7
Identity = 45/183 (24.59%), Postives = 83/183 (45.36%), Query Frame = -3
Query:  341 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRI---SVKYITADFRKTKIYDHIAESVEGL--PVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCML--KQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEID 868
            +K  G+ V VTG + GIG+    +LA+    L+L +R E +L    +  +E   +    +  +  D      ++   ++V      V +L+NN G +  S  F   D    R   ++   N L+ V + R +L   M     GG+++ +SS +    +P+   Y + K  +    N L++E+D
Sbjct:   46 LKLRGHVVWVTGASSGIGEALARELARCGCQLVLSARREAELQRVRKNCLEDSSLKESDILVLPMDVLDLSTHEACTKAVLDRFGRVDVLVNNAGRSQRSL-FMDTDLCVYRTLFEL---NVLAPVSLARCVLPGMMAPGSHGGILLAVSSIAGLAGVPLSAGYCASKHAVQGAFNALRSELD 224          
BLAST of SMED30021475 vs. Ensembl Yeast
Match: IFA38 (Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides [Source:SGD;Acc:S000000363])

HSP 1 Score: 117.857 bits (294), Expect = 6.231e-31
Identity = 78/253 (30.83%), Postives = 132/253 (52.17%), Query Frame = -3
Query:  170 FDKIKS-YGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKI--YDHIAESVEGLPVGLLINNVGAAY--PSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRIL-------LKSCMLKQG--GVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQL---CENHQSFGYIT 877
            FDK  +  G +  +TG +DGIGK F  ++AKR FNL+LISR + KL    +EL ++  + VK +  D  + K   Y+ I E    LP+ +L+NNVG ++  P P   T     +++  +++  N  +T+L+T+I+       +K+   K G  G+I+ M S    +  P+L  Y+  KS L  ++N L  E+      D I V+    + V S +S I+    ++P  ++F  S LR +   C + + +  +T
Sbjct:   54 FDKYGAKTGKYCAITGASDGIGKEFARQMAKRGFNLVLISRTQSKLEALQKELEDQHHVVVKILAIDIAEDKESNYESIKELCAQLPITVLVNNVGQSHSIPVPFLET----EEKELRNIITINNTATLLITQIIAPKIVETVKAENKKSGTRGLILTMGSFGGLIPTPLLATYSGSKSFLQGWSNSLAGELSK----DAIDVELIISYLVTSSMSKIRRSSLMIPNPQQFVKSTLRSVGRRCGSQERYATMT 298          
BLAST of SMED30021475 vs. Ensembl Yeast
Match: YMR226C (NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments [Source:SGD;Acc:S000004839])

HSP 1 Score: 51.6026 bits (122), Expect = 3.991e-8
Identity = 44/182 (24.18%), Postives = 79/182 (43.41%), Query Frame = -3
Query:  344 VVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIY---------DHIAESVEGLP-----VGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEI 847
            V++TG + GIGK             +  S  + KL++ ++ L EK     K I  +F   K++         + I   +E LP     + +L+NN G A  S        ++  D  D+   N  + + +T+ +L     K  G I+N+ S +     P  ++Y + K  +  FT+ L+ E+
Sbjct:   16 VLITGASAGIGKATA-------LEYLEASNGDMKLILAARRL-EKLEELKKTIDQEFPNAKVHVAQLDITQAEKIKPFIENLPQEFKDIDILVNNAGKALGSD---RVGQIATEDIQDVFDTNVTALINITQAVLPIFQAKNSGDIVNLGSIAGRDAYPTGSIYCASKFAVGAFTDSLRKEL 186          
BLAST of SMED30021475 vs. Ensembl Nematostella
Match: EDO41444 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4B0])

HSP 1 Score: 163.31 bits (412), Expect = 8.334e-48
Identity = 90/252 (35.71%), Postives = 147/252 (58.33%), Query Frame = -3
Query:  122 IKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADF-RKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTR--KFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL 868
            +K YG+W VVTG TDGIG+C+ EKLA R  N++LISR+ EKL    Q +  +F +  K I  DF    ++Y  + E +  L +G+L+NNVG A     F  AD L       ML+ N LS V+MT I+L   + +Q GV++N+SS      +P++++Y + K+ L+ F++CL +E  ++G    IFVQ   P +V +K++G++ +      PTA+++ +  L  +    ++ G+ +HS   W    +IP ++
Sbjct:   47 LKRYGSWAVVTGCTDGIGRCYAEKLAGRGLNIVLISRSLEKLKQVQQHIESQFSVQTKIIVKDFGGNAEVYQDLDEQLSNLDIGILVNNVGMASMINRF--AD-LKIEVCWKMLNVNALSAVMMTHIVLPGMLSRQRGVVVNVSSLVGSDPMPLMSVYCATKAFLDFFSSCLHSEYSSKG----IFVQCVRPAFVATKMTGMRNKPGTAFTPTADQYVEQALGTIGVEQRTSGFWSHSLMAWVTFNIIPAWV 291          
BLAST of SMED30021475 vs. Ensembl Nematostella
Match: EDO41442 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4A6])

HSP 1 Score: 142.51 bits (358), Expect = 7.912e-41
Identity = 78/233 (33.48%), Postives = 135/233 (57.94%), Query Frame = -3
Query:  152 VVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTK-IYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENW 847
            +VVTG TDGIGK F  +LA +  NLILISR +EKL     E+   +++  + I  DF  +  IY+ +   + GL +G+L+NNVG ++  P+F+T  ++   D   M++ N LS ++MT I+L   + +  G+++N+SS +     P+L++Y+SCK+ ++ F+ CL  E   +G    I VQ+  P +V +K+S I+     VP  +E+    L  +    ++ G  +H+ + +
Sbjct:    5 LVVTGCTDGIGKSFAYQLAAQGINLILISRTKEKLENMEAEIKSAYKVDTRIIALDFSGSADIYEGLDVKLGGLDIGILVNNVGVSH-YPEFFT--NMKREDCWKMINVNDLSVIMMTHIILPGMVSRGKGLVLNLSSGAGLEPRPLLSVYSSCKAFVDFFSCCLHDEYSGKG----IIVQSVMPLYVATKMSRIRKPNLFVPGPDEYVKQVLGTVGVQSRTNGCWSHALQVY 230          
BLAST of SMED30021475 vs. Ensembl Nematostella
Match: EDO41496 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4C5])

HSP 1 Score: 56.6102 bits (135), Expect = 3.901e-9
Identity = 63/274 (22.99%), Postives = 111/274 (40.51%), Query Frame = -3
Query:  104 LKPVSESKFDKIKSY-GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLP--VGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEF-----TDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVR 901
             + +++  F + KS  G  V++TG   GIG+     LAK+   L+L   N E L   +QE+ +       Y   D RK    + +A +VE     V +LINN G            + S+ D +     N L+ +   +  + S M K  G I++++S + +  +     Y + K         L+ EI   G     F      F      +G+K +   +P   E       +  +  +  N ++      S  +++L  +IP   + +LVR
Sbjct:   19 FQALAQFVFPRRKSLRGETVLITGAASGIGRLTALILAKKGCKLVLWDINLEALKAVAQEI-QDLGAETHYFECDVRKKDEVEKVANAVEDQAGNVTILINNAGVVTGKKFL----NCSESDVMRTFQVNSLAHIWTIQRFIPSMMEKNRGHIVSIASVAGYFGLVGCVDYCASKFAAVGLIEALRREIHGLGKMGIEFTTICPHFTATGMFAGVKLKYPNLPGLGELKPEFVAEKIVDGIETNSRTVFIPFMSRISYYLQTVIPTHSSDMLVR 287          
BLAST of SMED30021475 vs. Ensembl Nematostella
Match: EDO31654 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXC4])

HSP 1 Score: 53.9138 bits (128), Expect = 1.797e-8
Identity = 47/200 (23.50%), Postives = 91/200 (45.50%), Query Frame = -3
Query:  260 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGL-PVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKT 856
            G   +VTG + GIG    + LA+    + L +R EE+L     E++    +++   T    K ++   +  +   L PV +L++  G  Y    +    + + +D+  M+  NC   +     +L   + +  G I+ +SS++     P L +Y+  K  +   +  L+ EI    AG  + V    P  V++++ G+KT
Sbjct:    3 GQVAIVTGASSGIGAAVAKALAEAGVKVALAARREERLTQLRDEIIANGDVAISVKTDVTIKEQMESLVKRTDSTLGPVDILVSCAGVMY----YQMMKNCAMKDWEQMVDINCKGVMFGIGAVLPGMLERAKGHIVTISSSAGRKAFPGLAIYSGTKFFVEALSQGLRQEI----AGSGVKVTTIQPGDVVTEI-GLKT 193          
BLAST of SMED30021475 vs. Ensembl Nematostella
Match: EDO35908 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK77])

HSP 1 Score: 51.9878 bits (123), Expect = 1.072e-7
Identity = 51/190 (26.84%), Postives = 85/190 (44.74%), Query Frame = -3
Query:  344 KIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYD-HIAESVEGL------------PVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQG-GVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEI 871
            K +  G+  +VTG + GIG+    +LA++  ++ L++RN+ KL        E  R  V+      RK  ++   + +SVE +            PV LL N  G A         +     DF +M++ N L +V  T  ++K  M K+G G ++  SS      I  ++ Y+  K  +  F  CL  E+
Sbjct:   26 KTQINGSHAIVTGGSSGIGRALAVQLAQQGASVTLLARNKAKL--------EGARQRVEKHLQPGRKVSVFSVDLCQSVEAVQKVIDEACLALGPVNLLFNCAGYAVCG----VFEDTRPEDFKNMINTNYLGSVYPTYAVMKG-MKKRGCGHVVFTSSIGGQFGIFGMSAYSGSKFAVRGFAECLYMEV 202          
BLAST of SMED30021475 vs. Ensembl Medaka
Match: hsd17b12a (very-long-chain 3-oxoacyl-CoA reductase-A [Source:NCBI gene;Acc:101163971])

HSP 1 Score: 186.808 bits (473), Expect = 2.842e-56
Identity = 98/273 (35.90%), Postives = 157/273 (57.51%), Query Frame = -3
Query:   38 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIKNL*N 856
            G W VVTG TDGIGK + E+LA+R F ++LISR++EKL   ++ L E+F +  K I  DF KT IY  I   + GL +G+L+NNVG +Y  P++Y      D    +M++ N  S   MTR++L   + +  GVI+N+SSAS    +P+LT+Y++ K+ ++ F+  LQ E   QG    I +Q+  PF+V +K++ I+      PT E +  + L  +   +Q+ GY  H+   W   KL+P  +  + +  SM +++      R K++ + N
Sbjct:   56 GKWAVVTGATDGIGKSYAEELARRGFAMMLISRSQEKLDDVAKSLEEQFGVETKTIAVDFGKTDIYPKIEAGLVGLEIGVLVNNVGVSYHYPEYYLNIPDLDNFLTNMINVNMTSVCQMTRLVLPGMVSRAKGVILNISSASGMYPLPLLTVYSATKAFMDFFSRGLQEEYRRQG----IIIQSVLPFFVATKMTRIRKPTLDKPTPERYVAAELTTVGLQNQTNGYFPHAVMGWVTTKLVPTSI-VIFLGASMNRVQRSGYLHRRKLREMKN 323          
BLAST of SMED30021475 vs. Ensembl Medaka
Match: hsd17b12b (very-long-chain 3-oxoacyl-CoA reductase-B [Source:NCBI gene;Acc:101160884])

HSP 1 Score: 172.555 bits (436), Expect = 5.296e-51
Identity = 90/244 (36.89%), Postives = 145/244 (59.43%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRD-FID-MLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LA+R F+++LISR++EKL   S+ +  K  +  K I  DF    IY  I   + GL +G+L+NNVG +Y  P+F+   ++ + D FID M++ N  S   MTR++L   + ++ G I+N+SSAS    +P+LT+Y++ K+ ++ F+  LQAE  ++G    I +Q+  PF+V +KLS I+      P  + +  + +  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELARRGFSIVLISRSQEKLDEVSKAIAGKCGVETKTIAVDFSSVDIYPKIEAGLAGLEIGVLVNNVGISYSHPEFFL--NVPNLDTFIDTMVNINITSVCQMTRLVLPRMVERKKGAILNISSASGMYPVPLLTIYSASKAFVDFFSRGLQAEYKSKG----IIIQSVLPFFVATKLSKIRRATLDKPNPDRYVAAEINTVGLQTQTNGYLPHAVMGWVTTALLP 293          
BLAST of SMED30021475 vs. Ensembl Medaka
Match: hsd17b12b (very-long-chain 3-oxoacyl-CoA reductase-B [Source:NCBI gene;Acc:101160884])

HSP 1 Score: 163.696 bits (413), Expect = 8.775e-48
Identity = 86/242 (35.54%), Postives = 137/242 (56.61%), Query Frame = -3
Query:  131 GNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIP 856
            G W VVTG TDGIGK + E+LA+R F+++LISR++EKL   S+ +  K  +  K I  DF    IY  I   + GL +G+L+NNVG +Y  P+F+             L+   L T  MTR++L   + ++ G I+N+SSAS    +P+LT+Y++ K+ ++ F+  LQAE  ++G    I +Q+  PF+V +KLS I+      P  + +  + +  +    Q+ GY+ H+   W    L+P
Sbjct:   56 GKWAVVTGATDGIGKAYAEELARRGFSIVLISRSQEKLDEVSKAIAGKCGVETKTIAVDFSSVDIYPKIEAGLAGLEIGVLVNNVGISYSHPEFF-------------LNVPNLDT--MTRLVLPRMVERKKGAILNISSASGMYPVPLLTIYSASKAFVDFFSRGLQAEYKSKG----IIIQSVLPFFVATKLSKIRRATLDKPNPDRYVAAEINTVGLQTQTNGYLPHAVMGWVTTALLP 278          
BLAST of SMED30021475 vs. Ensembl Medaka
Match: hsd17b3 (hydroxysteroid 17-beta dehydrogenase 3 [Source:NCBI gene;Acc:100125485])

HSP 1 Score: 133.65 bits (335), Expect = 8.707e-36
Identity = 82/261 (31.42%), Postives = 139/261 (53.26%), Query Frame = -3
Query:  119 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPS--PDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLN 895
            P+SE+ F    S G W VVTG ++GIG+ +   LA+R  N++++SR +  L   ++E+       V+ I ADF    I+  I E ++ L VG+L+NNVG   PS  P  +      D+    +++CN  + V M R++L     ++ GVI+N+SS  + +  P+ T+YA+ K  +  F+  LQAE + +G    I +Q   PF V ++++G +    +    E+F  + L+ L   +++ G I H    W L  +  K L+
Sbjct:   69 PISETFF---TSMGEWAVVTGASEGIGRAYAFALAERGMNIVIMSRTKSTLDQVAKEISNSTGQKVRVIIADFTDENIFSEIEEQLKDLCVGILVNNVG-ILPSFIPYRFLESEDLDQTITKVINCNVKTMVKMCRMILPQMDKRRKGVIVNISSGIASVPFPLYTLYAASKIFVERFSQGLQAEYENRG----ILIQAVAPFGVCTRMAGYQPPNRVTFLPEDFVKNSLQYLRAGNKTHGSICHIVLGWILQCIPLKVLH 321          
BLAST of SMED30021475 vs. Ensembl Medaka
Match: hsd17b3 (hydroxysteroid 17-beta dehydrogenase 3 [Source:NCBI gene;Acc:100125485])

HSP 1 Score: 130.183 bits (326), Expect = 7.313e-35
Identity = 78/250 (31.20%), Postives = 133/250 (53.20%), Query Frame = -3
Query:  119 SYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPS--PDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLN 862
            S G W VVTG ++GIG+ +   LA+R  N++++SR +  L   ++E+       V+ I ADF    I+  I E ++ L VG+L+NNVG   PS  P  +      D+    +++CN  + V M R++L     ++ GVI+N+SS  + +  P+ T+YA+ K  +  F+  LQAE + +G    I +Q   PF V ++++G +    +    E+F  + L+ L   +++ G I H    W L  +  K L+
Sbjct:   49 SMGEWAVVTGASEGIGRAYAFALAERGMNIVIMSRTKSTLDQVAKEISNSTGQKVRVIIADFTDENIFSEIEEQLKDLCVGILVNNVG-ILPSFIPYRFLESEDLDQTITKVINCNVKTMVKMCRMILPQMDKRRKGVIVNISSGIASVPFPLYTLYAASKIFVERFSQGLQAEYENRG----ILIQAVAPFGVCTRMAGYQPPNRVTFLPEDFVKNSLQYLRAGNKTHGSICHIVLGWILQCIPLKVLH 293          
BLAST of SMED30021475 vs. Planmine SMEST
Match: SMESG000062708.1 (SMESG000062708.1)

HSP 1 Score: 625.55 bits (1612), Expect = 0.000e+0
Identity = 319/324 (98.46%), Postives = 321/324 (99.07%), Query Frame = -3
Query:   44 NCLVYLDYNLILYLCLIXXXXXXXXXXXXXXXVLWTYKLKPVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIKNL 1015
            N LVYLDYNLILYLCLI CVSF+FYHVVYFAAVLWTYKLKPVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIA SVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVI+NMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIKNL
Sbjct:    4 NFLVYLDYNLILYLCLIACVSFIFYHVVYFAAVLWTYKLKPVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAASVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIVNMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKIKNL 327          
BLAST of SMED30021475 vs. Planmine SMEST
Match: SMESG000077527.1 (SMESG000077527.1)

HSP 1 Score: 224.557 bits (571), Expect = 3.039e-71
Identity = 122/281 (43.42%), Postives = 171/281 (60.85%), Query Frame = -3
Query:   53 PVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLSGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLNKVLVRRSMMKLRDQASRKRDKI 895
            P   S   +I  YGNWVVVTG TDGIGK F E LAK KFNL+LISR+ EKL     E++EK  + ++ I+ADF K  IYD I   ++ L +GLLINNVG +   PD+ T++  S    I++L+CNCLSTVLMTR+LLK  M  +GG I+N+SSASS   IP L++Y S K+ +N FT  + +E       +++  QN  PF+V +K+S ++      PT   + +S L +L  +  ++G   H  + WF+  L+P F+ + LV       R +A RK  KI
Sbjct:   39 PFVFSSRQEINKYGNWVVVTGATDGIGKAFAELLAKDKFNLVLISRSFEKLEKVKNEILEKHNVDIRIISADFSKLDIYDKIESEIKDLEIGLLINNVGMSSKYPDYLTSEINSPDSVINILNCNCLSTVLMTRLLLKIRMADKGGFILNVSSASSMFPIPFLSVYGSSKAFINSFTRSMISEFR-----NKVHFQNVAPFFVSTKMSKLRP-SLTAPTPRNYANSVLNRLGTSTFTYGCFVHELQGWFIG-LLPSFVIEKLVYNVNYGTRIRAIRKATKI 312          
BLAST of SMED30021475 vs. Planmine SMEST
Match: SMESG000075064.1 (SMESG000075064.1)

HSP 1 Score: 147.517 bits (371), Expect = 1.014e-41
Identity = 105/287 (36.59%), Postives = 161/287 (56.10%), Query Frame = -3
Query:   80 LWTYKLKPVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSP-DFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKL-SGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLN----KVLVR--RSMMKLRD 916
            ++ YK    S      IK  G W VVTG TDGIG+ +C +LAK   N+ LISR+E+KL I S+EL + ++I V Y  ADF    IY+ + E ++GL +G+L+NNVG   P   DF   D         +++ N LS V+M++I+      ++ G+IIN+SSASS   +P+  +Y++ KS L  F+  L  E+   G    I VQ   PF+V +KL +  +  KF +   EEF +S +R +     +FG + HS +   +   IP F++    K++ R  +S +KLR+
Sbjct:   25 IFKYKFLLQSNKHVKLIKESGKWAVVTGATDGIGREYCRQLAKIGLNVFLISRDEKKLEITSKELKKCYKIDVMYFQADFASNSIYESLKERMKGLDIGVLVNNVGIQQPFVCDFIDIDE--SFSLSGIININILSAVMMSKIISAEMSQRKRGIIINISSASSLSPLPMAAIYSATKSFLTHFSLALNEELSKYG----ILVQTVRPFFVQTKLIAAYRLPKFFMSPVEEFVESAIRTVGTEGDTFGSLQHSIQGIIVRS-IPLFISIKIGKMIFRYFQSGLKLRE 304          
BLAST of SMED30021475 vs. Planmine SMEST
Match: SMESG000075064.1 (SMESG000075064.1)

HSP 1 Score: 147.517 bits (371), Expect = 1.077e-41
Identity = 105/287 (36.59%), Postives = 161/287 (56.10%), Query Frame = -3
Query:   80 LWTYKLKPVSESKFDKIKSYGNWVVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKLLIQSQELMEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSP-DFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKL-SGIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFLN----KVLVR--RSMMKLRD 916
            ++ YK    S      IK  G W VVTG TDGIG+ +C +LAK   N+ LISR+E+KL I S+EL + ++I V Y  ADF    IY+ + E ++GL +G+L+NNVG   P   DF   D         +++ N LS V+M++I+      ++ G+IIN+SSASS   +P+  +Y++ KS L  F+  L  E+   G    I VQ   PF+V +KL +  +  KF +   EEF +S +R +     +FG + HS +   +   IP F++    K++ R  +S +KLR+
Sbjct:   24 IFKYKFLLQSNKHVKLIKESGKWAVVTGATDGIGREYCRQLAKIGLNVFLISRDEKKLEITSKELKKCYKIDVMYFQADFASNSIYESLKERMKGLDIGVLVNNVGIQQPFVCDFIDIDE--SFSLSGIININILSAVMMSKIISAEMSQRKRGIIINISSASSLSPLPMAAIYSATKSFLTHFSLALNEELSKYG----ILVQTVRPFFVQTKLIAAYRLPKFFMSPVEEFVESAIRTVGTEGDTFGSLQHSIQGIIVRS-IPLFISIKIGKMIFRYFQSGLKLRE 303          
BLAST of SMED30021475 vs. Planmine SMEST
Match: SMESG000079254.1 (SMESG000079254.1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.127e-8
Identity = 53/198 (26.77%), Postives = 92/198 (46.46%), Query Frame = -3
Query:  275 VVVTGTTDGIGKCFCEKLAKRKFNLILISRNEEKL-----LIQSQELMEKFRISVKYITADFRKTKIYDHIAES--VEGLPVGLLINNVGAAYPSPDFYTADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFLEIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKL 847
             ++TG + GIGK   E   K   N+++  RN E L     L +  ++ E  RI     TAD    +    +AE   +E   + +LINN G    S D  T +++   +F  +++ N  S + +T  LL   ++ + G I+N+SS +     P +  Y   K+ L  FT C   ++ ++G    + V   +P   ++ L
Sbjct:   12 ALITGASSGIGKSTAELFFKLGANILITGRNLENLNDTIKLCELSKVNENQRIVA--FTADLTNEEDVKKLAEKTLLEFPKLDILINNAGVI--SND--TIENVEMENFDSVMNMNLRSVIQLTN-LLSPSLIDRKGSIVNVSSVAGTRAFPNVMSYCISKAALEQFTKCTALDLASKG----VRVNAVSPGVTVTNL 198          
The following BLAST results are available for this feature:
BLAST of SMED30021475 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HSD17B123.017e-5136.36hydroxysteroid 17-beta dehydrogenase 12 [Source:HG... [more]
HSDL14.894e-4733.84hydroxysteroid dehydrogenase like 1 [Source:HGNC S... [more]
HSD17B32.841e-4335.74hydroxysteroid 17-beta dehydrogenase 3 [Source:HGN... [more]
HSD17B32.692e-4235.74hydroxysteroid 17-beta dehydrogenase 3 [Source:HGN... [more]
HSD17B36.754e-3838.66hydroxysteroid 17-beta dehydrogenase 3 [Source:HGN... [more]
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BLAST of SMED30021475 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
stdh-11.588e-3930.18Putative steroid dehydrogenase 1 [Source:UniProtK... [more]
let-7672.584e-3930.43Very-long-chain 3-oxooacyl-coA reductase let-767 ... [more]
let-7672.584e-3930.43Very-long-chain 3-oxooacyl-coA reductase let-767 ... [more]
let-7674.568e-3930.43Very-long-chain 3-oxooacyl-coA reductase let-767 ... [more]
stdh-33.001e-3730.29Putative steroid dehydrogenase 3 [Source:UniProtK... [more]
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BLAST of SMED30021475 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spidey5.352e-4833.46gene:FBgn0029975 transcript:FBtr0071091[more]
CG132843.643e-4531.88gene:FBgn0032614 transcript:FBtr0345747[more]
CG132843.643e-4531.88gene:FBgn0032614 transcript:FBtr0080926[more]
CG132846.281e-4531.88gene:FBgn0032614 transcript:FBtr0332333[more]
CG132846.638e-4531.88gene:FBgn0032614 transcript:FBtr0332334[more]
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BLAST of SMED30021475 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hsd17b12b1.163e-5438.34hydroxysteroid (17-beta) dehydrogenase 12b [Source... [more]
hsd17b12a1.155e-5136.89hydroxysteroid (17-beta) dehydrogenase 12a [Source... [more]
hsd17b31.750e-4835.34hydroxysteroid (17-beta) dehydrogenase 3 [Source:Z... [more]
hsd17b33.350e-4834.98hydroxysteroid (17-beta) dehydrogenase 3 [Source:Z... [more]
hsd20b23.586e-4232.60hydroxysteroid (20-beta) dehydrogenase 2 [Source:Z... [more]
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BLAST of SMED30021475 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hsd17b121.011e-5437.86hydroxysteroid (17-beta) dehydrogenase 12 [Source:... [more]
hsd17b121.071e-5237.45hydroxysteroid (17-beta) dehydrogenase 12 [Source:... [more]
hsd17b121.830e-4937.45hydroxysteroid (17-beta) dehydrogenase 12 [Source:... [more]
hsd17b122.631e-4836.21hydroxysteroid (17-beta) dehydrogenase 12 [Source:... [more]
hsd17b121.223e-4635.39hydroxysteroid (17-beta) dehydrogenase 12 [Source:... [more]
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BLAST of SMED30021475 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Hsd17b124.017e-5535.58hydroxysteroid (17-beta) dehydrogenase 12 [Source:... [more]
Hsdl11.401e-4332.62hydroxysteroid dehydrogenase like 1 [Source:MGI Sy... [more]
Hsdl14.519e-4332.62hydroxysteroid dehydrogenase like 1 [Source:MGI Sy... [more]
Hsd17b31.475e-4131.92hydroxysteroid (17-beta) dehydrogenase 3 [Source:M... [more]
Hsd17b31.475e-4131.92hydroxysteroid (17-beta) dehydrogenase 3 [Source:M... [more]
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BLAST of SMED30021475 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O70503|DHB12_MOUSE2.810e-5435.58Very-long-chain 3-oxoacyl-CoA reductase OS=Mus mus... [more]
sp|Q28IU1|DHB12_XENTR5.356e-5437.86Very-long-chain 3-oxoacyl-CoA reductase OS=Xenopus... [more]
sp|Q6QA33|DH12B_DANRE8.778e-5438.34Very-long-chain 3-oxoacyl-CoA reductase-B OS=Danio... [more]
sp|Q6P7R8|DHB12_RAT3.862e-5336.73Very-long-chain 3-oxoacyl-CoA reductase OS=Rattus ... [more]
sp|Q4R5G7|DHB12_MACFA1.320e-5236.67Very-long-chain 3-oxoacyl-CoA reductase OS=Macaca ... [more]
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BLAST of SMED30021475 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3M7SUC42.946e-5937.96Very-long-chain 3-oxoacyl-reductase OS=Brachionus ... [more]
C3Y6162.367e-5838.83Uncharacterized protein OS=Branchiostoma floridae ... [more]
A0A3Q3KAP32.723e-5738.97Hydroxysteroid (17-beta) dehydrogenase 12a OS=Mono... [more]
A0A3P8QX524.634e-5637.64Hydroxysteroid (17-beta) dehydrogenase 12a OS=Asta... [more]
A0A3B4FSS64.634e-5637.64Hydroxysteroid (17-beta) dehydrogenase 12a OS=Pund... [more]
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BLAST of SMED30021475 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hsd17b12b2.151e-5635.96very-long-chain 3-oxoacyl-CoA reductase-B-like [So... [more]
hsd17b12a2.533e-5638.11hydroxysteroid 17-beta dehydrogenase 12 [Source:NC... [more]
hsd17b12a4.831e-5136.63hydroxysteroid 17-beta dehydrogenase 12 [Source:NC... [more]
hsd17b34.603e-4233.59hydroxysteroid 17-beta dehydrogenase 3 [Source:NCB... [more]
hsd20b25.527e-4232.58very-long-chain 3-oxoacyl-CoA reductase-like [Sour... [more]
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BLAST of SMED30021475 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hsd17b12b1.241e-4532.82hydroxysteroid (17-beta) dehydrogenase 12b [Source... [more]
hsd17b12b9.458e-4431.94hydroxysteroid (17-beta) dehydrogenase 12b [Source... [more]
dhrs7b2.288e-825.48dehydrogenase/reductase (SDR family) member 7B [So... [more]
kdsr4.225e-724.713-ketodihydrosphingosine reductase [Source:ZFIN;Ac... [more]
ENSPMAT00000010315.16.242e-724.59pep scaffold:Pmarinus_7.0:GL479994:4152:14615:1 ge... [more]
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BLAST of SMED30021475 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
IFA386.231e-3130.83Microsomal beta-keto-reductase; contains oleate re... [more]
YMR226C3.991e-824.18NADP(+)-dependent serine dehydrogenase and carbony... [more]
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BLAST of SMED30021475 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO414448.334e-4835.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414427.912e-4133.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414963.901e-922.99Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO316541.797e-823.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359081.072e-726.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30021475 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hsd17b12a2.842e-5635.90very-long-chain 3-oxoacyl-CoA reductase-A [Source:... [more]
hsd17b12b5.296e-5136.89very-long-chain 3-oxoacyl-CoA reductase-B [Source:... [more]
hsd17b12b8.775e-4835.54very-long-chain 3-oxoacyl-CoA reductase-B [Source:... [more]
hsd17b38.707e-3631.42hydroxysteroid 17-beta dehydrogenase 3 [Source:NCB... [more]
hsd17b37.313e-3531.20hydroxysteroid 17-beta dehydrogenase 3 [Source:NCB... [more]
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BLAST of SMED30021475 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062708.10.000e+098.46SMESG000062708.1[more]
SMESG000077527.13.039e-7143.42SMESG000077527.1[more]
SMESG000075064.11.014e-4136.59SMESG000075064.1[more]
SMESG000075064.11.077e-4136.59SMESG000075064.1[more]
SMESG000079254.12.127e-826.77SMESG000079254.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021475 ID=SMED30021475|Name=SMED30021475|organism=Schmidtea mediterranea sexual|type=transcript|length=1020bp
AAAGAAATAAGCTAATGATAAAAACCAATTATTAACAGTTTCAAAGGTTT
TTAATTTTATCTCTTTTCCTCGAAGCTTGGTCTCTTAATTTCATCATGCT
CCGACGCACTAAAACTTTATTGAGGAATTTCGGGATGAGCTTGTTCAAAA
ACCAGTTTTCATAAGAGTGAGTAATGTATCCAAACGATTGGTGATTTTCG
CATAGTTGTCGTAGACAAGAGTCTGTAAACTCCTCAGCGGTAGGTACCAA
AAATTTCCTCGTTTTTATGCCACTTAATTTTGATATCACCCAAAACGGGG
TGAAGTTCTGAACAAAAATCCGGTCTCCGGCTCCTTGGGCGTCAATTTCA
GCTTGTAAGCAGTTGGTAAAGCAGTTTAATAACGATTTGCATGAGGCGTA
CATGGTTAGTACTGGTATTTCTAGAAACGATGAAGCGGAAGACATATTAA
TAATAACGCCTCCTTGTTTGAGCATACATGATTTCAATAAAATTCTTGTC
ATTAATACAGTTGAAAGACAGTTGCAGTGAAGCATGTCAATGAAATCCCG
GTCCGACAAATGGTCAGCAGTGTAAAAATCTGGAGAAGGATATGCAGCTC
CGACATTGTTTATTAACAATCCAACGGGTAAACCTTCAACTGATTCTGCG
ATGTGATCGTAAATTTTCGTTTTTCTGAAGTCAGCAGTAATGTACTTTAC
TGAGATCCTGAATTTCTCCATTAGTTCCTGCGATTGAATGAGTAATTTTT
CTTCATTTCGACTTATTAGAATTAAATTGAACTTTCGTTTAGCGAGCTTT
TCACAGAAACACTTCCCAATTCCGTCAGTAGTTCCAGTAACGACGACCCA
ATTTCCATAGGATTTAATTTTGTCAAATTTGGATTCGGAAACCGGTTTAA
GTTTATAAGTCCACAAAACAGCAGCAAAATAGACAACGTGGTAAAATACA
AAACTTACACAGACAATCAAGCACAAGTAAAGAATTAAATTATAATCTAA
ATAAACTAGGCAATTTTAAA
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protein sequence of SMED30021475-orf-1

>SMED30021475-orf-1 ID=SMED30021475-orf-1|Name=SMED30021475-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=227bp
MEKFRISVKYITADFRKTKIYDHIAESVEGLPVGLLINNVGAAYPSPDFY
TADHLSDRDFIDMLHCNCLSTVLMTRILLKSCMLKQGGVIINMSSASSFL
EIPVLTMYASCKSLLNCFTNCLQAEIDAQGAGDRIFVQNFTPFWVISKLS
GIKTRKFLVPTAEEFTDSCLRQLCENHQSFGYITHSYENWFLNKLIPKFL
NKVLVRRSMMKLRDQASRKRDKIKNL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR036291NAD(P)-bd_dom_sf
IPR019774Aromatic-AA_hydroxylase_C
IPR002347SDR_fam
IPR016040NAD(P)-bd_dom
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: molecular function
TermDefinition
GO:0016714oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 307..324
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 49..294
e-value: 1.2E-36
score: 128.3
NoneNo IPR availablePIRSFPIRSF00012611-beta-HSD1coord: 4..320
e-value: 9.4E-72
score: 239.1
NoneNo IPR availablePANTHERPTHR43899:SF13RH59310Pcoord: 14..319
NoneNo IPR availablePANTHERPTHR43899FAMILY NOT NAMEDcoord: 14..319
NoneNo IPR availableCDDcd0535617beta-HSD1_like_SDR_ccoord: 54..298
e-value: 2.11934E-83
score: 250.213
NoneNo IPR availableTMHMMTMhelixcoord: 10..32
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 56..73
score: 33.95
coord: 206..225
score: 24.19
coord: 129..140
score: 32.45
coord: 180..196
score: 30.46
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 56..240
e-value: 3.1E-29
score: 101.8
IPR036291NAD(P)-binding domain superfamilySUPERFAMILYSSF51735NAD(P)-binding Rossmann-fold domainscoord: 54..306