TNF receptor associated factor-2

Overview
NameTNF receptor associated factor-2
Smed IDSMED30021446
Length (bp)762
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TNF receptor associated factor-2 (SMED30021446) t-SNE clustered cells

Violin plots show distribution of expression levels for TNF receptor associated factor-2 (SMED30021446) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TNF receptor associated factor-2 (SMED30021446) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TNF receptor associated factor-2 (SMED30021446) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021446

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30021446SMESG000049696.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000049545.1 SMESG000049503.1 dd_Smed_v4_3528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30021446SMESG000049696.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000049545.1 SMESG000049503.1 dd_Smed_v4_3528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021446SMESG000049696.1 SMESG000049649.1 SMESG000049621.1 SMESG000049603.1 SMESG000049545.1 SMESG000017435.1 SMESG000072318.1 SMESG000058718.1 SMESG000048483.1 SMESG000017533.1 SMESG000016079.1 SMESG000014105.1 SMESG000006480.1 SMESG000005275.1 SMESG000001086.1 Contig42692uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021446SMESG000049696.1 SMESG000049649.1 SMESG000049621.1 SMESG000049603.1 SMESG000049545.1 SMESG000017435.1 SMESG000072318.1 SMESG000058718.1 SMESG000048483.1 SMESG000017533.1 SMESG000016079.1 SMESG000014105.1 SMESG000006480.1 SMESG000005275.1 SMESG000001086.1 Contig42692newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30021446SMESG000015027.1 SMESG000070285.1 SMESG000049676.1 SMESG000049621.1 SMESG000015001.1 SMESG000069962.1 dd_Smed_v6_4528_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30021446SMESG000049688.1 SMESG000049676.1 SMESG000049663.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000065606.1 SMESG000065602.1 SMESG000065577.1 SMESG000017419.1 SMESG000016032.1 SMESG000015894.1 SMESG000000571.1 SMESG000000563.1 SMESG000000559.1 SMESG000000493.1 SMESG000000302.1 SMESG000000294.1 dd_Smed_v6_4144_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021446SMESG000049688.1 SMESG000049676.1 SMESG000049663.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000065606.1 SMESG000065602.1 SMESG000065577.1 SMESG000017419.1 SMESG000016032.1 SMESG000015894.1 SMESG000000571.1 SMESG000000563.1 SMESG000000559.1 SMESG000000493.1 SMESG000000302.1 SMESG000000294.1 dd_Smed_v6_4144_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Human
Match: TRAF6 (TNF receptor associated factor 6 [Source:HGNC Symbol;Acc:HGNC:12036])

HSP 1 Score: 55.4546 bits (132), Expect = 2.735e-8
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP +       +I     +  D+     I  ++VKCP + C  K EL  +E+H   C+   + CP
Sbjct:   59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD------NEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Human
Match: TRAF6 (TNF receptor associated factor 6 [Source:HGNC Symbol;Acc:HGNC:12036])

HSP 1 Score: 55.4546 bits (132), Expect = 2.735e-8
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP +       +I     +  D+     I  ++VKCP + C  K EL  +E+H   C+   + CP
Sbjct:   59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD------NEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Human
Match: LNX2 (ligand of numb-protein X 2 [Source:HGNC Symbol;Acc:HGNC:20421])

HSP 1 Score: 48.9062 bits (115), Expect = 4.342e-6
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = 3 
Query:   42 RENK-FESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCP 200
            REN  +   NE+  DL+C IC   +++   TPCG  FC  C+  +L+E D FCP
Sbjct:   33 RENHLYNYQNEVDDDLVCHICLQPLLQPLDTPCGHTFCYKCLRNFLQEKD-FCP 85
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Human
Match: LNX2 (ligand of numb-protein X 2 [Source:HGNC Symbol;Acc:HGNC:20421])

HSP 1 Score: 48.9062 bits (115), Expect = 4.342e-6
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = 3 
Query:   42 RENK-FESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCP 200
            REN  +   NE+  DL+C IC   +++   TPCG  FC  C+  +L+E D FCP
Sbjct:   33 RENHLYNYQNEVDDDLVCHICLQPLLQPLDTPCGHTFCYKCLRNFLQEKD-FCP 85
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Zebrafish
Match: lnx1 (ligand of numb-protein X 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9439])

HSP 1 Score: 52.373 bits (124), Expect = 2.398e-7
Identity = 31/110 (28.18%), Postives = 54/110 (49.09%), Query Frame = 3 
Query:   36 MMREN-KFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCP-TQNCETKTELSMMEEHIR 359
            +  EN ++    ++  DL+C IC   +I+   TPCG  +C +C+  +L E+D FCP +     +QK   +  +      +  + +++V CP   +C        ME HIR
Sbjct:   38 LQEENHEYTYKEDVDDDLMCHICLQPLIRPLDTPCGHTYCQECLTNFLLESD-FCPVDRTPLMLQKCRKSSLL-----VHKLLDKLMVSCPFADHCTEVMPRGEMEGHIR 141
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Zebrafish
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 51.6026 bits (122), Expect = 3.993e-7
Identity = 32/111 (28.83%), Postives = 47/111 (42.34%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   +  A  TPCG RFC  CI + +++    CP +       ++     +  D+     I  + VKC    C  K EL  +E+H+  C      CP
Sbjct:   60 EFDPPLESKYECPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVD------NEVLLEEQLFPDNFAKREILSLTVKCSNFGCSEKMELRQLEKHLSQCRFATAPCP 164
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Zebrafish
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 51.6026 bits (122), Expect = 3.993e-7
Identity = 32/111 (28.83%), Postives = 47/111 (42.34%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   +  A  TPCG RFC  CI + +++    CP +       ++     +  D+     I  + VKC    C  K EL  +E+H+  C      CP
Sbjct:   60 EFDPPLESKYECPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVD------NEVLLEEQLFPDNFAKREILSLTVKCSNFGCSEKMELRQLEKHLSQCRFATAPCP 164
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Zebrafish
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 51.6026 bits (122), Expect = 3.993e-7
Identity = 32/111 (28.83%), Postives = 47/111 (42.34%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   +  A  TPCG RFC  CI + +++    CP +       ++     +  D+     I  + VKC    C  K EL  +E+H+  C      CP
Sbjct:   60 EFDPPLESKYECPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVD------NEVLLEEQLFPDNFAKREILSLTVKCSNFGCSEKMELRQLEKHLSQCRFATAPCP 164
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Zebrafish
Match: lnx2a (ligand of numb-protein X 2a [Source:ZFIN;Acc:ZDB-GENE-060228-2])

HSP 1 Score: 50.447 bits (119), Expect = 8.664e-7
Identity = 21/53 (39.62%), Postives = 33/53 (62.26%), Query Frame = 3 
Query:   69 EISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQ 227
            E+  DL+C IC   +++   TPCG  FC+ C+  +L+E D FCP +  + Q+Q
Sbjct:   42 EVDDDLVCHICLQPLVQPLDTPCGHTFCARCLRSFLQERD-FCPLDRAHLQLQ 93
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Xenopus
Match: egf (epidermal growth factor [Source:Xenbase;Acc:XB-GENE-951823])

HSP 1 Score: 55.8398 bits (133), Expect = 2.098e-8
Identity = 34/110 (30.91%), Postives = 54/110 (49.09%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISC 386
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP + +     ++ F  N ++    ++R     VKCP+Q C    EL  +E H+  CD   + C
Sbjct:   61 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACILKSIRDAGHKCPVDNESLMENQL-FPDNFAKREILSLR-----VKCPSQGCTETMELRHLERHLVRCDFAGVEC 164
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Xenopus
Match: ddx54 (DEAD-box helicase 54 [Source:Xenbase;Acc:XB-GENE-1011576])

HSP 1 Score: 49.2914 bits (116), Expect = 3.046e-6
Identity = 32/108 (29.63%), Postives = 55/108 (50.93%), Query Frame = 3 
Query:   42 RENK-FESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCP-TQNCETKTELSMMEEHIR 359
            REN  +   NE+  DL+C IC   +++   TPCG  FC  C+  +L+E D FCP + K     +++F          +  + +++V CP +  C+   +   ME H++
Sbjct:   31 RENHLYNYQNEVDDDLVCHICLQPLLQPLDTPCGHTFCFKCLRNFLQEKD-FCPLDRK-----RLHFKLCKKSSILVHKLLDKLVVSCPYSAACKETMQRCDMEAHLK 132
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Mouse
Match: Traf6 (TNF receptor-associated factor 6 [Source:MGI Symbol;Acc:MGI:108072])

HSP 1 Score: 55.4546 bits (132), Expect = 2.034e-8
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP +       +I     +  D+     I  + VKCP + C  K EL  +E+H   C+   ++CP
Sbjct:   59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD------NEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. UniProt/SwissProt
Match: sp|Q3ZCC3|TRAF6_BOVIN (TNF receptor-associated factor 6 OS=Bos taurus OX=9913 GN=TRAF6 PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.908e-8
Identity = 36/111 (32.43%), Postives = 54/111 (48.65%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP +       +I     +  D+     I  ++VKCP + C  K EL  +EEH   C+   +SCP
Sbjct:   60 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD------NEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEEHQAHCEFALMSCP 164
[Close]
BLAST of TNF receptor associated factor-2 vs. UniProt/SwissProt
Match: sp|B6CJY4|TRAF6_CERAT (TNF receptor-associated factor 6 OS=Cercocebus atys OX=9531 GN=TRAF6 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 7.773e-8
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP +       +I     +  D+     I  ++VKCP + C  K EL  +E+H   C+   + CP
Sbjct:   59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD------NEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALVDCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. UniProt/SwissProt
Match: sp|B6CJY5|TRAF6_MACMU (TNF receptor-associated factor 6 OS=Macaca mulatta OX=9544 GN=TRAF6 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 7.991e-8
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP +       +I     +  D+     I  ++VKCP + C  K EL  +E+H   C+   + CP
Sbjct:   59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD------NEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALVDCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. UniProt/SwissProt
Match: sp|Q28DL4|TRAF6_XENTR (TNF receptor-associated factor 6 OS=Xenopus tropicalis OX=8364 GN=traf6 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.164e-7
Identity = 34/110 (30.91%), Postives = 54/110 (49.09%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISC 386
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP + +     ++ F  N ++    ++R     VKCP+Q C    EL  +E H+  CD   + C
Sbjct:   61 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACILKSIRDAGHKCPVDNESLMENQL-FPDNFAKREILSLR-----VKCPSQGCTETMELRHLERHLVRCDFAGVEC 164
[Close]
BLAST of TNF receptor associated factor-2 vs. UniProt/SwissProt
Match: sp|Q9Y4K3|TRAF6_HUMAN (TNF receptor-associated factor 6 OS=Homo sapiens OX=9606 GN=TRAF6 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.314e-7
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            E D  + S   C IC   + +A  TPCG RFC  CI + +++  + CP +       +I     +  D+     I  ++VKCP + C  K EL  +E+H   C+   + CP
Sbjct:   59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD------NEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: I1ZID8 (TNF receptor associated factor-2 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 3.531e-35
Identity = 66/189 (34.92%), Postives = 112/189 (59.26%), Query Frame = 3 
Query:   33 KMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQI 599
            +++    FE +  +  +L C +CK L++ AC + CGCRFCS CI +Y+   + +CPG    C+ + +    N+  D+  N +I+++ VKCP + C+   EL  +EEH+R C  +  +CPF  +GC   ++  +++  HL    + HSKLIID+I+N RN+   L  + +ELR ++  L K + QS+ +I
Sbjct:    8 RLLITYNFEVEGGVPDNLKCDLCKNLLVNACQSRCGCRFCSKCIMEYVSRGNLYCPGGTSECKEENLTICSNIHTDYRVNRKIAKMRVKCPEETCKVYCELVDIEEHLRLCTMKSQNCPFFVLGCNQPKMVIQDLTNHLQNGTFQHSKLIIDIISNMRNDFFGLTTKEVELREDIN-LIKNHFQSQNEI 195
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: T1E101 (TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.989e-23
Identity = 54/151 (35.76%), Postives = 85/151 (56.29%), Query Frame = 3 
Query:   63 DNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEM 515
            D  +++DLLC +C  LM  AC + CGC +C  CI  YL +N N+CPG+   C+   +N   +V  +   + RIS ++VKCP   C   +EL  ++EH+  C    + CP  +IGCE   +  +EM  HL +    H++LI+   N  ++E+
Sbjct:   22 DESVNNDLLCILCNKLMRDACESACGCHYCYTCIRIYLSQNHNYCPGDKTECKEDYLNLEGDVFRNIRLSTRISRIVVKCPNIRCNYTSELLRLKEHLDKCTPESVECPCVTIGCEEEPLLKDEMAKHLTDQVLRHTQLIMSFCNELKSEI 172
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: A0A0F7GWW3 (TNF receptor associated factor 4 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.580e-19
Identity = 49/142 (34.51%), Postives = 86/142 (60.56%), Query Frame = 3 
Query:  237 FTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENME-------LRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKM 641
               N+  D+P N++I+++ VKC  ++C+   EL  ME+H RTC  R ++CP+++IGC+   I+ +++  HL    + H+KL++D + NF NEME LK  N         LRNE+ ELK    + + +I +++ +  NQ +++
Sbjct:   12 LNENICVDYPMNMKIAKLQVKCKEKSCDYVDELRKMEDHSRTCKYRSVACPYSNIGCDKCNISQDKVTDHLQSEIFVHTKLLMDFVVNFNNEMEMLKANNNGLSPVNELLRNEINELKLDGDRRRVEIGDLQRKCANQAEEI 153
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: A0A1B6J2U8 (Uncharacterized protein OS=Homalodisca liturata OX=320908 GN=g.6975 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 9.044e-9
Identity = 47/176 (26.70%), Postives = 84/176 (47.73%), Query Frame = 3 
Query:   33 KMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDK-----RPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMEL 545
            KM+ E ++E          C IC   +     T CG RFC +CI Q+LK+ D  CP ++      +++ T+++  D+ T   I++    CP + C     L+ +E+H+   ++       I C F+++GC     +  ++NAHLN   + H +L+   I+  R      K +  +L
Sbjct:   14 KMLIEPRYE----------CPICLTCLKDPLLTSCGHRFCQECIHQWLKQEDGTCPIDSY-----QLSITKDLFPDNYTRREIAQQRRNCPVEECSQLVPLTELEKHVTIHEEYRQQNENIMCTFHNVGCHTQLQSIHQLNAHLNSQLHHHLELLSSAISEGRKGQVDAKAQEAQL 174
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: A0A1B6FGM2 (Uncharacterized protein (Fragment) OS=Cuerna arida OX=1464854 GN=g.14975 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.086e-8
Identity = 47/167 (28.14%), Postives = 82/167 (49.10%), Query Frame = 3 
Query:   21 TNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDK-----RPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFR 506
            TN  KM+ E ++E          C IC   +     T CG RFC +CI Q+LK+ D  CP ++      +++ T+++  D+ T   I++    CP + C     L+ +E+H+   ++       I C F+++GC     +  ++NAHLN   + H +L+   I+  R
Sbjct:   10 TNNSKMLIEPRYE----------CPICLTCLKDPLLTSCGHRFCQECIHQWLKQEDGTCPIDSY-----QLSITKDLFPDNYTRREIAQQRRNCPVEECSQLVPLTELEKHVTIHEEYRQQNENIMCTFHNVGCNTQLHSIHQLNAHLNSQLHHHLELLSSAISEGR 161
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826])

HSP 1 Score: 49.6766 bits (117), Expect = 1.338e-6
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 3 
Query:   36 MMREN-KFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCP 200
            ++ EN ++    E   DLLC IC   +I+   TPCG  +C +C+  +L E+D FCP
Sbjct:  267 LLDENHEYNYKEEADDDLLCHICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCP 321
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826])

HSP 1 Score: 49.6766 bits (117), Expect = 1.351e-6
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 3 
Query:   36 MMREN-KFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCP 200
            ++ EN ++    E   DLLC IC   +I+   TPCG  +C +C+  +L E+D FCP
Sbjct:  267 LLDENHEYNYKEEADDDLLCHICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCP 321
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826])

HSP 1 Score: 49.6766 bits (117), Expect = 1.351e-6
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 3 
Query:   36 MMREN-KFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCP 200
            ++ EN ++    E   DLLC IC   +I+   TPCG  +C +C+  +L E+D FCP
Sbjct:  267 LLDENHEYNYKEEADDDLLCHICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCP 321
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Cavefish
Match: traf6 (TNF receptor associated factor 6 [Source:NCBI gene;Acc:103021325])

HSP 1 Score: 48.9062 bits (115), Expect = 2.241e-6
Identity = 34/114 (29.82%), Postives = 50/114 (43.86%), Query Frame = 3 
Query:   63 DNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDH--PTNIRISEVI---VKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            D  + S   C IC   +  A  TPCG RFC+ CI + +++    CP + +            ++ED   P N    E++   V+C    C  K EL  +E HI  C    + CP
Sbjct:   61 DPPLESKYECPICLMGLRSAVQTPCGHRFCNCCIRKSIRDTGQKCPVDNEV-----------LTEDQLFPDNFAKREILSLTVRCRNVGCMEKMELRHLENHIPHCKFATVPCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Cavefish
Match: traf6 (TNF receptor associated factor 6 [Source:NCBI gene;Acc:103021325])

HSP 1 Score: 48.9062 bits (115), Expect = 2.299e-6
Identity = 34/114 (29.82%), Postives = 50/114 (43.86%), Query Frame = 3 
Query:   63 DNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDH--PTNIRISEVI---VKCPTQNCETKTELSMMEEHIRTCDKRPISCP 389
            D  + S   C IC   +  A  TPCG RFC+ CI + +++    CP + +            ++ED   P N    E++   V+C    C  K EL  +E HI  C    + CP
Sbjct:   61 DPPLESKYECPICLMGLRSAVQTPCGHRFCNCCIRKSIRDTGQKCPVDNEV-----------LTEDQLFPDNFAKREILSLTVRCRNVGCMEKMELRHLENHIPHCKFATVPCP 163
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Sea Lamprey
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 45.4394 bits (106), Expect = 6.964e-6
Identity = 33/117 (28.21%), Postives = 51/117 (43.59%), Query Frame = 3 
Query:   51 KFESDNE--ISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVI---VKCPTQNCETKTELSMMEEHIRTCDKRPISC 386
             +E+D E  + S   C IC   + +   TPCG RFC  CI   ++  D         C V   N   +  +  P N    E++   V+CP + C  + EL  ++EH   C+   + C
Sbjct:   50 GYEADFEPPLESKYECPICLMALREPVQTPCGHRFCRACILHSMRLRD-----AGPKCPVD--NAVLHEGQLFPDNFAKREILSLTVRCPNKPCGHRGELRALQEHQLRCEYAQVPC 159
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Nematostella
Match: EDO41243 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z7])

HSP 1 Score: 52.7582 bits (125), Expect = 4.530e-8
Identity = 29/106 (27.36%), Postives = 51/106 (48.11%), Query Frame = 3 
Query:   72 ISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNF-CPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISC 386
            ++ +  CRIC   +     T CG RFC +C+++  +      CP +      + ++  ++V  D      I   +VKCP + C+ + EL  +E H R CD   ++C
Sbjct:   19 VAEEYTCRICHLPLRVPVQTKCGHRFCKECLDEASRRKSRAECPMDR-----EPLDLKKDVFLDKAAERTILSFLVKCPCKGCKWEAELRDLEAHARNCDYELVTC 119
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Nematostella
Match: EDO43806 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXN6])

HSP 1 Score: 46.9802 bits (110), Expect = 6.665e-7
Identity = 31/112 (27.68%), Postives = 52/112 (46.43%), Query Frame = 3 
Query:   69 EISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQY----LKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQ--NCETKTELSMMEEHIRTCDKRPISC 386
            ++  +LLC IC  ++ +A  TPCG  FC  C+E +    L EN+  CP     C+     +  +          +  +IV CP     C+   +L  +E H+++C   P+ C
Sbjct:   11 KVDPNLLCGICAEVLERAVLTPCGHSFCGVCLETWMNAKLGENEK-CPASCPSCRADL--YQGDTIPVLALRGIVDGLIVHCPNADNGCKLVLKLEGVEGHLKSCSHAPVQC 119
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Nematostella
Match: EDO27426 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T9A1])

HSP 1 Score: 45.0542 bits (105), Expect = 5.553e-6
Identity = 26/101 (25.74%), Postives = 47/101 (46.53%), Query Frame = 3 
Query:   72 ISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNF-CPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDK 371
            ++ +  CRIC   +     T CG RFC +C+++  +      CP        + ++  ++V  D      I   +VKCP + C+ + EL  +E  ++ C K
Sbjct:   19 VAEEYTCRICHLPLRVPVQTKCGHRFCKECLDEASRRKSRAECP-----MDREPLDLKKDVFLDKAAERTILSFLVKCPCKGCKWEAELRDLEVSMKGCGK 114
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Medaka
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:101156395])

HSP 1 Score: 54.299 bits (129), Expect = 4.128e-8
Identity = 31/113 (27.43%), Postives = 59/113 (52.21%), Query Frame = 3 
Query:   33 KMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNI---RISEVIVKCP-TQNCETKTELSMMEEHIR 359
            K+   +++   +E+  DL+C+IC   +I+   TPCG  +C +C+  +L E+D FCP       +Q        S   P+ +    + ++++ CP T++C  K     +E+HI+
Sbjct:   47 KLEENHEYLYQDEVDDDLVCQICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCPVCRDPLMLQ--------SCRKPSLLVHKLLDKLMITCPFTEHCTEKLARGQLEDHIK 150
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Medaka
Match: traf6 (TNF receptor associated factor 6 [Source:NCBI gene;Acc:101161132])

HSP 1 Score: 51.9878 bits (123), Expect = 2.227e-7
Identity = 35/115 (30.43%), Postives = 52/115 (45.22%), Query Frame = 3 
Query:   57 ESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDH--PTNIRISEVI---VKCPTQNCETKTELSMMEEHIRTCDKRPISC 386
            E D  + S   C IC   +  A  T CG RFC  CIE+ +++    CP + +            +SED   P N    E++   V+CP  +C  K EL  +E+H+  C    + C
Sbjct:   67 EFDPPLESKYECPICLMALRNAIQTRCGHRFCKSCIEKSIRDTGQRCPVDNEM-----------LSEDQLFPDNFAKREILSLTVRCPNSDCGEKMELRHLEKHLAQCQFATVPC 170
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Medaka
Match: lnx2a (ligand of numb-protein X 2 [Source:NCBI gene;Acc:101160604])

HSP 1 Score: 48.1358 bits (113), Expect = 4.174e-6
Identity = 21/54 (38.89%), Postives = 32/54 (59.26%), Query Frame = 3 
Query:   69 EISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQK 230
            E+  DL+C IC   +++   TPCG  FC+ C+  +L+E D FCP +    Q+Q 
Sbjct:   42 EVDDDLVCHICLQPLVQPLDTPCGHTFCARCLRSFLQERD-FCPLDRTRLQLQA 94
[Close]
BLAST of TNF receptor associated factor-2 vs. Ensembl Medaka
Match: lnx2b (ligand of Numb protein X 2 [Source:NCBI gene;Acc:101170936])

HSP 1 Score: 47.3654 bits (111), Expect = 7.647e-6
Identity = 20/59 (33.90%), Postives = 36/59 (61.02%), Query Frame = 3 
Query:   54 FESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQK 230
            +E  +E+  +L+C IC   ++K   TPCG  +C  C+  +LK+ D FCP + +Y + ++
Sbjct:   37 YEYLDEVDDELICHICLQPLLKPMDTPCGHTYCFYCLSNFLKDQD-FCPVDRQYLEFEQ 94
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049621.1 (SMESG000049621.1)

HSP 1 Score: 437.187 bits (1123), Expect = 1.442e-156
Identity = 244/249 (97.99%), Postives = 247/249 (99.20%), Query Frame = 3 
Query:   15 MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRR 761
            MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKE+DNFCPGEAKYCQVQKINFTRNVSEDHP N+RISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCE+NEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKK NESLREEMTELRR
Sbjct:    1 MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKEDDNFCPGEAKYCQVQKINFTRNVSEDHPANLRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCEMNEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKANESLREEMTELRR 249
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049621.1 (SMESG000049621.1)

HSP 1 Score: 436.802 bits (1122), Expect = 9.039e-155
Identity = 244/249 (97.99%), Postives = 247/249 (99.20%), Query Frame = 3 
Query:   15 MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRR 761
            MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKE+DNFCPGEAKYCQVQKINFTRNVSEDHP N+RISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCE+NEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKK NESLREEMTELRR
Sbjct:    1 MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKEDDNFCPGEAKYCQVQKINFTRNVSEDHPANLRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCEMNEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKANESLREEMTELRR 249
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049676.1 (SMESG000049676.1)

HSP 1 Score: 434.491 bits (1116), Expect = 2.448e-152
Identity = 244/249 (97.99%), Postives = 247/249 (99.20%), Query Frame = 3 
Query:   15 MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRR 761
            MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHP N+RISEVIVKCPTQNCETKTELSMMEEH+RTCDKRPISCPFNSIGCEINEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQ KQQIDNIEFQS+NQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRR
Sbjct:    1 MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPANLRISEVIVKCPTQNCETKTELSMMEEHMRTCDKRPISCPFNSIGCEINEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQCKQQIDNIEFQSSNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRR 249

HSP 2 Score: 58.151 bits (139), Expect = 1.567e-9
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:  492 INNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQ 662
            I     E+ ++K EN  LR EMTEL+++NR+ KQQ DNI+  STNQ QKM EMEA++
Sbjct:  223 IKKINKEIVAVKKENESLREEMTELRRENREFKQQFDNIQSLSTNQQQKMGEMEARE 279
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049621.1 (SMESG000049621.1)

HSP 1 Score: 421.394 bits (1082), Expect = 1.762e-150
Identity = 237/242 (97.93%), Postives = 240/242 (99.17%), Query Frame = 3 
Query:   36 MMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRR 761
            MMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKE+DNFCPGEAKYCQVQKINFTRNVSEDHP N+RISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCE+NEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKK NESLREEMTELRR
Sbjct:    1 MMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKEDDNFCPGEAKYCQVQKINFTRNVSEDHPANLRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCEMNEIANEEMNAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKANESLREEMTELRR 242
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049567.1 (SMESG000049567.1)

HSP 1 Score: 428.328 bits (1100), Expect = 4.924e-150
Identity = 243/249 (97.59%), Postives = 244/249 (97.99%), Query Frame = 3 
Query:   15 MFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFCSDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRR 761
            MFTNLWKMMRENKFESDNEISSDLLCRICK LMIKACHTPCGCRFCSDCIEQYL E D FCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPF SIGCEINEIANEE+NAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRR
Sbjct:    1 MFTNLWKMMRENKFESDNEISSDLLCRICKRLMIKACHTPCGCRFCSDCIEQYLNETDKFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFISIGCEINEIANEEINAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRR 249

HSP 2 Score: 59.6918 bits (143), Expect = 4.064e-10
Identity = 32/57 (56.14%), Postives = 42/57 (73.68%), Query Frame = 3 
Query:  492 INNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQ 662
            I     E+ ++K EN  LR EMTEL+++NR+ KQQIDNI+  STNQ QKM EMEA++
Sbjct:  223 IKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEMEARE 279
The following BLAST results are available for this feature:
BLAST of TNF receptor associated factor-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TRAF62.735e-830.63TNF receptor associated factor 6 [Source:HGNC Symb... [more]
TRAF62.735e-830.63TNF receptor associated factor 6 [Source:HGNC Symb... [more]
LNX24.342e-642.59ligand of numb-protein X 2 [Source:HGNC Symbol;Acc... [more]
LNX24.342e-642.59ligand of numb-protein X 2 [Source:HGNC Symbol;Acc... [more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lnx12.398e-728.18ligand of numb-protein X 1 [Source:ZFIN;Acc:ZDB-GE... [more]
traf63.993e-728.83TNF receptor-associated factor 6 [Source:NCBI gene... [more]
traf63.993e-728.83TNF receptor-associated factor 6 [Source:NCBI gene... [more]
traf63.993e-728.83TNF receptor-associated factor 6 [Source:NCBI gene... [more]
lnx2a8.664e-739.62ligand of numb-protein X 2a [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
egf2.098e-830.91epidermal growth factor [Source:Xenbase;Acc:XB-GEN... [more]
ddx543.046e-629.63DEAD-box helicase 54 [Source:Xenbase;Acc:XB-GENE-1... [more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Traf62.034e-830.63TNF receptor-associated factor 6 [Source:MGI Symbo... [more]
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BLAST of TNF receptor associated factor-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3ZCC3|TRAF6_BOVIN1.908e-832.43TNF receptor-associated factor 6 OS=Bos taurus OX=... [more]
sp|B6CJY4|TRAF6_CERAT7.773e-830.63TNF receptor-associated factor 6 OS=Cercocebus aty... [more]
sp|B6CJY5|TRAF6_MACMU7.991e-830.63TNF receptor-associated factor 6 OS=Macaca mulatta... [more]
sp|Q28DL4|TRAF6_XENTR1.164e-730.91TNF receptor-associated factor 6 OS=Xenopus tropic... [more]
sp|Q9Y4K3|TRAF6_HUMAN1.314e-730.63TNF receptor-associated factor 6 OS=Homo sapiens O... [more]
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BLAST of TNF receptor associated factor-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZID83.531e-3534.92TNF receptor associated factor-2 (Fragment) OS=Sch... [more]
T1E1011.989e-2335.76TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
A0A0F7GWW36.580e-1934.51TNF receptor associated factor 4 (Fragment) OS=Sch... [more]
A0A1B6J2U89.044e-926.70Uncharacterized protein OS=Homalodisca liturata OX... [more]
A0A1B6FGM22.086e-828.14Uncharacterized protein (Fragment) OS=Cuerna arida... [more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lnx11.338e-639.29ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
lnx11.351e-639.29ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
lnx11.351e-639.29ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
traf62.241e-629.82TNF receptor associated factor 6 [Source:NCBI gene... [more]
traf62.299e-629.82TNF receptor associated factor 6 [Source:NCBI gene... [more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
traf66.964e-628.21TNF receptor-associated factor 6 [Source:NCBI gene... [more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO412434.530e-827.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO438066.665e-727.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO274265.553e-625.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
lnx14.128e-827.43ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
traf62.227e-730.43TNF receptor associated factor 6 [Source:NCBI gene... [more]
lnx2a4.174e-638.89ligand of numb-protein X 2 [Source:NCBI gene;Acc:1... [more]
lnx2b7.647e-633.90ligand of Numb protein X 2 [Source:NCBI gene;Acc:1... [more]
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BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000049621.11.442e-15697.99SMESG000049621.1[more]
SMESG000049621.19.039e-15597.99SMESG000049621.1[more]
SMESG000049676.12.448e-15297.99SMESG000049676.1[more]
SMESG000049621.11.762e-15097.93SMESG000049621.1[more]
SMESG000049567.14.924e-15097.59SMESG000049567.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021446 ID=SMED30021446|Name=TNF receptor associated factor-2|organism=Schmidtea mediterranea sexual|type=transcript|length=762bp
ATTATAAAGACAAAATGTTTACAAACTTATGGAAAATGATGAGAGAAAAT
AAATTTGAATCTGATAATGAAATCAGCAGTGATTTGTTATGCAGAATTTG
CAAATGTTTAATGATAAAAGCTTGTCATACCCCTTGCGGATGTCGTTTTT
GTTCGGATTGTATTGAACAATATTTGAAAGAAAATGATAATTTCTGTCCA
GGAGAGGCAAAATATTGTCAAGTTCAAAAGATTAATTTCACTAGGAATGT
TTCGGAGGATCACCCAACTAATATAAGGATATCAGAAGTCATTGTGAAAT
GCCCAACACAGAATTGTGAAACCAAAACCGAGTTGAGCATGATGGAGGAG
CACATAAGAACATGCGACAAACGACCAATCAGCTGTCCATTCAATTCAAT
TGGCTGTGAAATAAATGAAATAGCCAACGAGGAAATGAATGCACATCTCA
ATGAGAGCAATTATTGGCATAGCAAACTGATTATTGATTTAATCAATAAT
TTCCGCAATGAGATGGAATCACTGAAGAATGAAAATATGGAATTGAGAAA
TGAAATGACCGAATTGAAGAAGAAAAACAGACAATCCAAACAACAAATTG
ATAATATCGAATTTCAGTCAACAAATCAATATCAGAAAATGAGCGAGATG
GAAGCGAAACAGCTGGAAAATGCTGAAGAAATAAAGAAAATCAATAAAGA
AATTGTAGCAGTGAAGAAGGAAAATGAATCATTGAGAGAAGAAATGACGG
AATTGAGGAGGG
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protein sequence of SMED30021446-orf-1

>SMED30021446-orf-1 ID=SMED30021446-orf-1|Name=SMED30021446-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=253bp
YKDKMFTNLWKMMRENKFESDNEISSDLLCRICKCLMIKACHTPCGCRFC
SDCIEQYLKENDNFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKC
PTQNCETKTELSMMEEHIRTCDKRPISCPFNSIGCEINEIANEEMNAHLN
ESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQID
NIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTE
LRR
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000429neoblast
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR043211TNF_rcpt-assoc_TRAF
IPR013083Znf_RING/FYVE/PHD
IPR001293Znf_TRAF
IPR013323SIAH-type
IPR001841Znf_RING
IPR008974TRAF-like
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0004842ubiquitin-protein transferase activity
GO:0005515protein binding
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0016567protein ubiquitination
GO:0006955immune response
GO:0007165signal transduction
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 155..203
NoneNo IPR availableCOILSCoilCoilcoord: 225..251
NoneNo IPR availablePANTHERPTHR10131:SF129TNF RECEPTOR-ASSOCIATED FACTOR 6coord: 11..120
NoneNo IPR availablePANTHERPTHR10131:SF129TNF RECEPTOR-ASSOCIATED FACTOR 6coord: 112..213
NoneNo IPR availableCDDcd16449RING-HCcoord: 28..64
e-value: 3.01054E-7
score: 43.9953
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 20..65
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 15..85
e-value: 1.0E-7
score: 33.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 91..147
e-value: 2.4E-7
score: 32.9
IPR001293Zinc finger, TRAF-typePFAMPF02176zf-TRAFcoord: 93..145
e-value: 1.8E-7
score: 31.7
IPR001293Zinc finger, TRAF-typePROSITEPS50145ZF_TRAFcoord: 96..133
score: 8.71
IPR043211TNF receptor-associated factor TRAFPANTHERPTHR10131TNF RECEPTOR ASSOCIATED FACTORcoord: 11..120
IPR043211TNF receptor-associated factor TRAFPANTHERPTHR10131TNF RECEPTOR ASSOCIATED FACTORcoord: 112..213
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 28..64
score: 9.229
IPR008974TRAF-likeSUPERFAMILYSSF49599TRAF domain-likecoord: 94..167