SMED30021434
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021434 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of SMED30021434 vs. Ensembl Human
Match: ZBED8 (zinc finger BED-type containing 8 [Source:HGNC Symbol;Acc:HGNC:30804]) HSP 1 Score: 110.153 bits (274), Expect = 4.042e-51 Identity = 69/242 (28.51%), Postives = 120/242 (49.59%), Query Frame = -3 Query: 1039 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCI-SSEDITLKTTHLKNLRGF 1755 +F F + ++ I + + +DGA SM+G+ F+AY+K E+P I HC + L+ K + +L + L ++ IN IK +A N RLF ++ Y+ LL HTE+RWLS+G L +++ + +F + + N FK H+ LAD++ N +Q +N + A+ + F K + + ++ F F L ++ V + + I + +ITL HL+ L F Sbjct: 219 VFNLFRDFFLKHKIALDVCGSVCTDGASSMLGENSEFVAYVKKEIPHIVVTHCLLNPHALVIKTLPTKLRDALFTVVRVINFIKGRAPNHRLFQAFFEEIGIEYSVLLFHTEMRWLSRGQILTHIFEMYEEINQFLHHKSSNLVDGFENKEFKIHLAYLADLFKHLNELSASMQRTGMNTVSAREKLSAFVRKFPFWQKRIEKRNFTNFPFLEEIIVSDNEGIFIAAEITL---HLQQLSNF 457 HSP 2 Score: 82.4185 bits (202), Expect = 4.042e-51 Identity = 53/138 (38.41%), Postives = 80/138 (57.97%), Query Frame = -2 Query: 1730 LEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS--KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL-LF*KYF 2131 L+ASY+ AYL AK PHT+ E L+ P E+ VL + + LQ +PLS+ + RID+M+++I Q++ +++ S K I+L E T S LM +VRY + EI+EEF + L+ KG D+ LF +F Sbjct: 92 LQASYQFAYLCAKEKNPHTVAEKLVKPCALEIAQIVLGPDAQKKLQQVPLSDDVIHSRIDEMSQDILQQVLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRY--IKEREIVEEFLFCEPLQLSMKGIDVFNLFRDFF 227 HSP 3 Score: 38.1206 bits (87), Expect = 4.042e-51 Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = -3 Query: 2218 KIRQYSEEYLKFGFVIAPV--N*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQTSK 2406 K R++ ++Y+++ F P C++C FSN ++PS L +H NR H +L + K Sbjct: 3 KKRKWDDDYVRYWFTCTTEVDGTQRPQCVLCNSVFSNADLRPSKLSDHFNRQHGGVAGHDLNSLK 67 HSP 4 Score: 34.6538 bits (78), Expect = 4.042e-51 Identity = 23/81 (28.40%), Postives = 40/81 (49.38%), Query Frame = -1 Query: 759 WIIDPFLCDLTRFDIS--VHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 995 WI+DPFL ++ D S + +L L+ Q + F T FW + +P+L + +L+ F ++Y E FS++ Sbjct: 473 WILDPFLFNIDFVDDSYLMKNDLAELRASGQILMEFETMKLEDFWCAQFT--AFPNLAKTALEILMPFATTYLCELGFSSL 551
BLAST of SMED30021434 vs. Ensembl Human
Match: ZBED8 (zinc finger BED-type containing 8 [Source:HGNC Symbol;Acc:HGNC:30804]) HSP 1 Score: 110.153 bits (274), Expect = 4.042e-51 Identity = 69/242 (28.51%), Postives = 120/242 (49.59%), Query Frame = -3 Query: 1039 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCI-SSEDITLKTTHLKNLRGF 1755 +F F + ++ I + + +DGA SM+G+ F+AY+K E+P I HC + L+ K + +L + L ++ IN IK +A N RLF ++ Y+ LL HTE+RWLS+G L +++ + +F + + N FK H+ LAD++ N +Q +N + A+ + F K + + ++ F F L ++ V + + I + +ITL HL+ L F Sbjct: 219 VFNLFRDFFLKHKIALDVCGSVCTDGASSMLGENSEFVAYVKKEIPHIVVTHCLLNPHALVIKTLPTKLRDALFTVVRVINFIKGRAPNHRLFQAFFEEIGIEYSVLLFHTEMRWLSRGQILTHIFEMYEEINQFLHHKSSNLVDGFENKEFKIHLAYLADLFKHLNELSASMQRTGMNTVSAREKLSAFVRKFPFWQKRIEKRNFTNFPFLEEIIVSDNEGIFIAAEITL---HLQQLSNF 457 HSP 2 Score: 82.4185 bits (202), Expect = 4.042e-51 Identity = 53/138 (38.41%), Postives = 80/138 (57.97%), Query Frame = -2 Query: 1730 LEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS--KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL-LF*KYF 2131 L+ASY+ AYL AK PHT+ E L+ P E+ VL + + LQ +PLS+ + RID+M+++I Q++ +++ S K I+L E T S LM +VRY + EI+EEF + L+ KG D+ LF +F Sbjct: 92 LQASYQFAYLCAKEKNPHTVAEKLVKPCALEIAQIVLGPDAQKKLQQVPLSDDVIHSRIDEMSQDILQQVLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRY--IKEREIVEEFLFCEPLQLSMKGIDVFNLFRDFF 227 HSP 3 Score: 38.1206 bits (87), Expect = 4.042e-51 Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = -3 Query: 2218 KIRQYSEEYLKFGFVIAPV--N*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQTSK 2406 K R++ ++Y+++ F P C++C FSN ++PS L +H NR H +L + K Sbjct: 3 KKRKWDDDYVRYWFTCTTEVDGTQRPQCVLCNSVFSNADLRPSKLSDHFNRQHGGVAGHDLNSLK 67 HSP 4 Score: 34.6538 bits (78), Expect = 4.042e-51 Identity = 23/81 (28.40%), Postives = 40/81 (49.38%), Query Frame = -1 Query: 759 WIIDPFLCDLTRFDIS--VHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 995 WI+DPFL ++ D S + +L L+ Q + F T FW + +P+L + +L+ F ++Y E FS++ Sbjct: 473 WILDPFLFNIDFVDDSYLMKNDLAELRASGQILMEFETMKLEDFWCAQFT--AFPNLAKTALEILMPFATTYLCELGFSSL 551
BLAST of SMED30021434 vs. Ensembl Human
Match: FAM200B (family with sequence similarity 200 member B [Source:HGNC Symbol;Acc:HGNC:27740]) HSP 1 Score: 100.138 bits (248), Expect = 2.479e-45 Identity = 65/211 (30.81%), Postives = 105/211 (49.76%), Query Frame = -3 Query: 1120 ILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEV--PEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTL--NRKEFRYFDKLMQ 1734 I+ QY + KN +SDG +M GK+ + L +W HCF HR+ L ++ I + L VL +K +N IK +LNSRL C + + +LL HT++RWLS+G L R ++ + + F E + + + + + T + L DI+ + N KLQG+N ++ I FR + L+ L NR + F + +Q Sbjct: 280 IVGQYKLNWKNCKGITSDGTATMTGKHSRVIKKLLEVTNNGAVWN-HCFIHREGLASREIPQNLMEVLKNAVKVVNFIKGSSLNSRLLETFCSEIGTNHTHLLYHTKIRWLSQGKILSRVYELRNEIHFFLIEKKSHLASIFEDDTWVTKLAYLTDIFSILNELSLKLQGKNSDVFQHVERIQGFRKTLLLWQVRLKSNRPSYYMFPRFLQ 489 HSP 2 Score: 73.559 bits (179), Expect = 2.479e-45 Identity = 53/157 (33.76%), Postives = 89/157 (56.69%), Query Frame = -2 Query: 1748 FLTRSNKVIK---SFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS-KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2203 + R K IK F+ ST+ + E L +SY +AY +AK +T E ++LP +++ T+ + L+ +P +++TV RI +AE++E LIT L++ FAI+LDE T G + L++YVRY +++ +E+F C +L + G DI Sbjct: 121 YFQRKKKDIKLSTQFLSCSTAVS-EKALLSSYLVAYRVAKEKIANTAAEKIILPACLDMVRTIFDDKSADKLKTIP-NDNTVSLRICTIAEHLETMLITRLQSGIDFAIQLDESTDIGSCTTLLVYVRY--AWQDDFLEDFLCFLNLTSHLSGLDIF 273 HSP 3 Score: 52.373 bits (124), Expect = 2.479e-45 Identity = 26/59 (44.07%), Postives = 37/59 (62.71%), Query Frame = -3 Query: 2248 KKKI--RQYSEEYLKFGFVIA--PVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHAD 2412 KKK+ R+Y+E+YLK+GF+ P P C+IC +N+ +KPS LK HL HA+ Sbjct: 55 KKKVSARRYNEDYLKYGFIKCEKPFENDRPQCVICNNILANESLKPSKLKRHLETQHAE 113
BLAST of SMED30021434 vs. Ensembl Human
Match: FAM200B (family with sequence similarity 200 member B [Source:HGNC Symbol;Acc:HGNC:27740]) HSP 1 Score: 100.138 bits (248), Expect = 2.479e-45 Identity = 65/211 (30.81%), Postives = 105/211 (49.76%), Query Frame = -3 Query: 1120 ILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEV--PEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTL--NRKEFRYFDKLMQ 1734 I+ QY + KN +SDG +M GK+ + L +W HCF HR+ L ++ I + L VL +K +N IK +LNSRL C + + +LL HT++RWLS+G L R ++ + + F E + + + + + T + L DI+ + N KLQG+N ++ I FR + L+ L NR + F + +Q Sbjct: 280 IVGQYKLNWKNCKGITSDGTATMTGKHSRVIKKLLEVTNNGAVWN-HCFIHREGLASREIPQNLMEVLKNAVKVVNFIKGSSLNSRLLETFCSEIGTNHTHLLYHTKIRWLSQGKILSRVYELRNEIHFFLIEKKSHLASIFEDDTWVTKLAYLTDIFSILNELSLKLQGKNSDVFQHVERIQGFRKTLLLWQVRLKSNRPSYYMFPRFLQ 489 HSP 2 Score: 73.559 bits (179), Expect = 2.479e-45 Identity = 53/157 (33.76%), Postives = 89/157 (56.69%), Query Frame = -2 Query: 1748 FLTRSNKVIK---SFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS-KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2203 + R K IK F+ ST+ + E L +SY +AY +AK +T E ++LP +++ T+ + L+ +P +++TV RI +AE++E LIT L++ FAI+LDE T G + L++YVRY +++ +E+F C +L + G DI Sbjct: 121 YFQRKKKDIKLSTQFLSCSTAVS-EKALLSSYLVAYRVAKEKIANTAAEKIILPACLDMVRTIFDDKSADKLKTIP-NDNTVSLRICTIAEHLETMLITRLQSGIDFAIQLDESTDIGSCTTLLVYVRY--AWQDDFLEDFLCFLNLTSHLSGLDIF 273 HSP 3 Score: 52.373 bits (124), Expect = 2.479e-45 Identity = 26/59 (44.07%), Postives = 37/59 (62.71%), Query Frame = -3 Query: 2248 KKKI--RQYSEEYLKFGFVIA--PVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHAD 2412 KKK+ R+Y+E+YLK+GF+ P P C+IC +N+ +KPS LK HL HA+ Sbjct: 55 KKKVSARRYNEDYLKYGFIKCEKPFENDRPQCVICNNILANESLKPSKLKRHLETQHAE 113
BLAST of SMED30021434 vs. Ensembl Human
Match: FAM200A (family with sequence similarity 200 member A [Source:HGNC Symbol;Acc:HGNC:25401]) HSP 1 Score: 109.768 bits (273), Expect = 1.290e-40 Identity = 75/231 (32.47%), Postives = 118/231 (51.08%), Query Frame = -3 Query: 1093 IFYCFEN-ILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVP--EIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTL--NRKEFRYFDKLMQ---LSVIEKDCI 1755 +F EN +L QY + K+ SSDG +M GK+ L +W HCF HR+ L++K I L +VL +K +N IK +LNSRL C + + +LL HTEVRWLS+G L R ++ + + F E + + + + T + L+DI+G+ N K+QG+N +I I F+ + L+ + L NR + F L+Q ++I +DC+ Sbjct: 187 LFTELENCLLGQYKLNWKHCKGISSDGTANMTGKHSRLTEKLLEATHNNAVWN-HCFIHREALVSKEISPSLMDVLKNAVKTVNFIKGSSLNSRLLEIFCSEIGVNHTHLLFHTEVRWLSQGKVLSRVYELRNEIYIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLKMQGKNNDIFQYLEHILGFQKTLLLWQARLKSNRPSYYMFPTLLQHIEENIINEDCL 416 HSP 2 Score: 79.7221 bits (195), Expect = 1.290e-40 Identity = 53/157 (33.76%), Postives = 88/157 (56.05%), Query Frame = -2 Query: 1748 NFLTRSNKVIKS--FMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS-KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2206 +F NKV S + ST+ N E L +SY +AY +AK HT E ++LP +++ T+ + L+ +PLS++T+ RRI +A+++E LIT L++ FAI+LDE T L++YVRY +++ +E+ C +L + G D+ Sbjct: 35 HFQKERNKVESSPQVLSRSTTMN-ERALLSSYLVAYRVAKEKMAHTAAEKIILPACMDMVRTIFDDKSADKLRTIPLSDNTISRRICTIAKHLEAMLITRLQSGIDFAIQLDESTDIASCPTLLVYVRY--VWQDDFVEDLLCCLNLNSHITGLDLF 188
BLAST of SMED30021434 vs. Ensembl Zebrafish
Match: CR392001.3 (pep chromosome:GRCz11:8:38963323:38965260:-1 gene:ENSDARG00000117159.1 transcript:ENSDART00000181495.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR392001.3) HSP 1 Score: 106.301 bits (264), Expect = 2.864e-27 Identity = 70/229 (30.57%), Postives = 107/229 (46.72%), Query Frame = -3 Query: 1099 EKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPE-IWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFEND-------NTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKD 1761 E + C L + I ++I+ ++DGAPSM G RGF+ L+ + + HC H++ L A+ E V+NL I+ +N I AKALN R F L D+ D Y++LLLH +VRWLSK L+RF+ V F D +T + L+F + D+ N LQ R + + +F K+ +F + R +F L + +D Sbjct: 228 EAVLKC----LNENGINTNHLISVATDGAPSMRGSKRGFVTLLQKALDRNLLAFHCILHQEALCAQTFPSECMVVMNLVIEMVNKIIAKALNHRQFRALLDEVDSEYSDLLLHNKVRWLSKDEVLRRFVACLEHVKTFLKSKDLIYPQLEDTEWLEKLHF------MVDMTSHLNKLNESLQVRGNTALQMLEAVLSFERKLTVFARDVQRGTLSHFPSLREFKESHQD 446 HSP 2 Score: 33.4982 bits (75), Expect = 2.864e-27 Identity = 47/158 (29.75%), Postives = 75/158 (47.47%), Query Frame = -2 Query: 1751 RSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHT----IRENLLLPVISEVITTVLNQNDRPVLQ---NLPLSNSTVQRRIDKMAENIELQLITELRTS-KFAIKLDE*TFGRNSILMLYVRYYSCS--KEEIIEEFFCDKSLETCTKGEDI 2194 R NK K P+++ AS+ I K G P T ++E+ + ISE + + +N ++Q ++PLS TV+ R KMA NI Q I ++ ++ ++I DE + L RY + +EEII+ L+ T+GEDI Sbjct: 87 RKNKFKKWIASPNSTT------AASFVATREIIKRGKPFTDGDYMKESFI--NISEHLFSDF-KNKTEIIQKIKDMPLSAKTVKERAIKMAGNITEQQIKDINSAPAYSIACDE-----SCDTALLCRYVNSDGPQEEIIKLI----PLKGQTRGEDI 226 HSP 3 Score: 24.2534 bits (51), Expect = 2.864e-27 Identity = 11/31 (35.48%), Postives = 17/31 (54.84%), Query Frame = -3 Query: 2251 SIPLCLICEKSFSNDYMKPSILKNHLNRMHA 2343 +P CL+C + SN+ K S ++ H HA Sbjct: 33 GLPECLLCSEKLSNN--KKSNVERHFQGRHA 61
BLAST of SMED30021434 vs. Ensembl Zebrafish
Match: AL928808.1 (pep chromosome:GRCz11:20:17257101:17259573:-1 gene:ENSDARG00000101333.2 transcript:ENSDART00000166397.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL928808.1) HSP 1 Score: 105.531 bits (262), Expect = 4.225e-25 Identity = 60/213 (28.17%), Postives = 116/213 (54.46%), Query Frame = -3 Query: 1114 NILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLK--NEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDE-VYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFK--THIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLS 1737 ++++ ++ + + A +DGAP+M+G RG + K + P WT HC H++H ++K ++ L +++ ++ +N ++ ALN R F L D+ DE + ++LL H VRWLS+G L RF ++ + V+ F + +L + + + + L D+ N LQG+ + D +F F +K+ LF + L ++++ +F L++ S Sbjct: 239 TVVEKANLQLSKLTAIVTDGAPAMLGSERGLVGLCKADDRFPAFWTFHCIIHQEHWVSKKLN--LDHIMKPVLEIVNFVRTHALNHRQFKNLIDELDEDLPSDLLFHCAVRWLSRGHVLSRFFELLNP-VKLFLAEKHKEYPELHDPQWISDLAFLVDVLHYLNGLNVDLQGKLKMLPDLVQSVFAFVNKLKLFKTHLQKRDYTHFPTLLKAS 448 HSP 2 Score: 31.187 bits (69), Expect = 4.225e-25 Identity = 27/104 (25.96%), Postives = 49/104 (47.12%), Query Frame = -1 Query: 759 EDFNVRFRDMENLCVP-AWIIDPFLCD-------LTRFDISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 1046 + F RF +++ ++I+PF + L + + E+I L ED + + + +FW I V E+YP++ + LL +F S+Y E FS + Sbjct: 471 QSFEERFSNLQQKRQQITFLINPFTAESGCLKAPLVEDEAASQLEMIELSEDDRLKSVLR-EGTVEFWKI-VPVERYPNVKQAALKLLSMFGSTYVCELLFSTL 572
BLAST of SMED30021434 vs. Ensembl Xenopus
Match: spag1 (sperm associated antigen 1 [Source:Xenbase;Acc:XB-GENE-853609]) HSP 1 Score: 90.5077 bits (223), Expect = 1.023e-17 Identity = 58/196 (29.59%), Postives = 101/196 (51.53%), Query Frame = -3 Query: 1117 SDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKA--KALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFF--FENDNTMTTQL--LNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQL 1686 +DGA +MVG+ +G L+ E + HC H++ L ++ ++ +++++ K N I+ ++L R F ++ D Y +LLLHT VRWLS G CL RF + + + + D+ + L ++F T + L DI N+ LQGR+ N+ + I FR+K+ L S L + + +F+ +L Sbjct: 258 TDGARAMVGRNQGLAGRLRKEGIDCHMFHCIVHQEVLCGTSL--KMADIMDVVTKVTNLIRGGNRSLTHRRFKNFLEELDAAYGDLLLHTNVRWLSAGKCLVRFFALRKEIHLYLSDIKCDSYLLEHLTDVSFLTALAFLTDITQFLNSLNLNLQGRDQNVSQLYSHIAAFRNKLHLLKSFLAKNDLTHFESCCEL 451
BLAST of SMED30021434 vs. Ensembl Xenopus
Match: ENSXETT00000016563.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:37379994:37381838:-1 gene:ENSXETG00000009412.1 transcript:ENSXETT00000016563.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 90.5077 bits (223), Expect = 1.167e-17 Identity = 58/196 (29.59%), Postives = 101/196 (51.53%), Query Frame = -3 Query: 1117 SDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKA--KALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFF--FENDNTMTTQL--LNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQL 1686 +DGA +MVG+ +G L+ E + HC H++ L ++ ++ +++++ K N I+ ++L R F ++ D Y +LLLHT VRWLS G CL RF + + + + D+ + L ++F T + L DI N+ LQGR+ N+ + I FR+K+ L S L + + +F+ +L Sbjct: 258 TDGARAMVGRNQGLAGRLRKEGIDCHMFHCIVHQEVLCGTSL--KMADIMDVVTKVTNLIRGGNRSLTHRRFKNFLEELDAAYGDLLLHTNVRWLSAGKCLVRFFALRKEIHLYLSDIKCDSYLLEHLTDVSFLTALAFLTDITQFLNSLNLNLQGRDQNVSQLYSHIAAFRNKLHLLKSFLAKNDLTHFESCCEL 451
BLAST of SMED30021434 vs. Ensembl Mouse
Match: Zbed5 (zinc finger, BED type containing 5 [Source:MGI Symbol;Acc:MGI:1919220]) HSP 1 Score: 121.709 bits (304), Expect = 6.899e-48 Identity = 72/220 (32.73%), Postives = 111/220 (50.45%), Query Frame = -3 Query: 1126 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFF-------FE---NDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKL 1755 +F ++ L+Q++I K+I +DGAP+ +G GF + NE P+ HC H Q L K + ++ V+ + ++N +KA +LNSRLF QLC DE LLLHTE RWLS+G L+R ++ + FF FE +DN+ + + L DI+ + N LQG N +D I +F+ K+ L+ L+ +F L Sbjct: 381 VFEAVDSFLRQHEISWKSICGVCTDGAPATLGCQSGFQRLVLNESPKAIGAHCMLHLQTLAMKTLPQDFQEVMKSVLSSVNFVKASSLNSRLFLQLCSDLDEPSKTLLLHTEGRWLSRGKVLKRIFELRDELKMFFNQKAIRQFEALFSDNSALQK-------VAYLVDIFTILNELNLSLQGPNSTCLDLSEKIHSFQMKLQLWQKKLDENKFYMLPTL 593 HSP 2 Score: 72.4034 bits (176), Expect = 6.899e-48 Identity = 49/141 (34.75%), Postives = 76/141 (53.90%), Query Frame = -2 Query: 1748 STSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKF---AIKLDE*TFGRNSI-LMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2158 S +NV +E SY +A IA+ PHT E+LL PV + + ++ L + LSN TV+RRI M+ +I Q++ E++++ +I+LDE T N + LM+YVRY + + +EF C K LE D+ Sbjct: 245 SNKQNV-AAVETSYLVALRIARDMKPHTFAEHLLFPVAKDRVRVMIGDEFVTKLSAVSLSNDTVRRRIHDMSADILDQVVQEIKSAPLPICSIQLDESTDVANCLQLMVYVRYI--NDGDFKDEFLCCKPLERTATALDVF 382 HSP 3 Score: 39.6614 bits (91), Expect = 6.899e-48 Identity = 20/53 (37.74%), Postives = 29/53 (54.72%), Query Frame = -3 Query: 2251 KIRQYSEEYLKFGFVIA-PVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHA 2406 K R+Y+E +L++GF V P C+IC S + MKP+ LK H H+ Sbjct: 165 KRRKYNEGFLQYGFTSTITVGIERPQCVICGVVLSAESMKPNKLKRHFESKHS 217
BLAST of SMED30021434 vs. Ensembl Mouse
Match: Zmym6 (zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI:106505]) HSP 1 Score: 78.9518 bits (193), Expect = 4.989e-33 Identity = 60/222 (27.03%), Postives = 103/222 (46.40%), Query Frame = -3 Query: 1171 LKEKIFYCFENILKQYDIPI---------------KNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWT-----VHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFK---THIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLF 1767 +KE++ +C E D+ + + + +DGA SM +Y L++++ EI HCF HR+HL AK + LH +L + ++ +K A +S++ LC++ + NL L+ EVRWLS+G L R ++ +E F ++ + + + + LADI+ L N + LQG T FN +K+D+F Sbjct: 770 VKEELLFCTEMPSPSTDLEVFELINKYIDSRSLNWNHCVGFCTDGAASMTDRY----FRLRSKIQEIAKNTVTFTHCFIHREHLAAKKLSPCLHEILLQSSQILSFVKNSASDSQMLTILCEEMGSEHVNLPLNAEVRWLSRGRILTRLFELRHE-IEIFLNQKHSDLARYFHDEEWIAKLAYLADIFSLINKLNSSLQG-------TMTTFFNLYNKVDVF 979 HSP 2 Score: 70.4774 bits (171), Expect = 4.989e-33 Identity = 43/120 (35.83%), Postives = 74/120 (61.67%), Query Frame = -2 Query: 1790 EGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSK-FAIKLDE*TFGRN-SILMLYVRYYSCSKEEIIEE-FFC 2140 E ++ASY IA+ IA P +I E L+ P + E+ + VL + ++ +PLSNST+ RI K++++IE QL+ ++R S+ FA+++DE + +L+ YVR+ + ++ EE FC Sbjct: 658 ESLVKASYLIAFQIAARNKPFSIAEELIKPYLVEMCSEVLGSSAGEKMKTIPLSNSTIGCRIKKLSDDIEDQLLQKVRESRWFALQVDESSEATEVPLLLCYVRFIDYDRGDVKEELLFC 777 HSP 3 Score: 34.6538 bits (78), Expect = 4.989e-33 Identity = 17/54 (31.48%), Postives = 29/54 (53.70%), Query Frame = -3 Query: 2248 RQYSEEYLKFGFVIAPVN*SIPL---CLICEKSFSNDYMKPSILKNHLNRMHAD 2400 + Y+ EY++FGF+I + C+IC + ++ + P L NHL H+D Sbjct: 574 QTYNAEYIRFGFIICSGSEESSPSPQCVICGEVLPSESVMPVSLSNHLKAKHSD 627
BLAST of SMED30021434 vs. Ensembl Mouse
Match: Zmym6 (zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI:106505]) HSP 1 Score: 78.9518 bits (193), Expect = 5.360e-33 Identity = 60/222 (27.03%), Postives = 103/222 (46.40%), Query Frame = -3 Query: 1171 LKEKIFYCFENILKQYDIPI---------------KNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWT-----VHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFK---THIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLF 1767 +KE++ +C E D+ + + + +DGA SM +Y L++++ EI HCF HR+HL AK + LH +L + ++ +K A +S++ LC++ + NL L+ EVRWLS+G L R ++ +E F ++ + + + + LADI+ L N + LQG T FN +K+D+F Sbjct: 862 VKEELLFCTEMPSPSTDLEVFELINKYIDSRSLNWNHCVGFCTDGAASMTDRY----FRLRSKIQEIAKNTVTFTHCFIHREHLAAKKLSPCLHEILLQSSQILSFVKNSASDSQMLTILCEEMGSEHVNLPLNAEVRWLSRGRILTRLFELRHE-IEIFLNQKHSDLARYFHDEEWIAKLAYLADIFSLINKLNSSLQG-------TMTTFFNLYNKVDVF 1071 HSP 2 Score: 70.0922 bits (170), Expect = 5.360e-33 Identity = 43/120 (35.83%), Postives = 74/120 (61.67%), Query Frame = -2 Query: 1790 EGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSK-FAIKLDE*TFGRN-SILMLYVRYYSCSKEEIIEE-FFC 2140 E ++ASY IA+ IA P +I E L+ P + E+ + VL + ++ +PLSNST+ RI K++++IE QL+ ++R S+ FA+++DE + +L+ YVR+ + ++ EE FC Sbjct: 750 ESLVKASYLIAFQIAARNKPFSIAEELIKPYLVEMCSEVLGSSAGEKMKTIPLSNSTIGCRIKKLSDDIEDQLLQKVRESRWFALQVDESSEATEVPLLLCYVRFIDYDRGDVKEELLFC 869 HSP 3 Score: 34.6538 bits (78), Expect = 5.360e-33 Identity = 17/54 (31.48%), Postives = 29/54 (53.70%), Query Frame = -3 Query: 2248 RQYSEEYLKFGFVIAPVN*SIPL---CLICEKSFSNDYMKPSILKNHLNRMHAD 2400 + Y+ EY++FGF+I + C+IC + ++ + P L NHL H+D Sbjct: 666 QTYNAEYIRFGFIICSGSEESSPSPQCVICGEVLPSESVMPVSLSNHLKAKHSD 719
BLAST of SMED30021434 vs. Ensembl Mouse
Match: Gtf2ird2 (GTF2I repeat domain containing 2 [Source:MGI Symbol;Acc:MGI:2149780]) HSP 1 Score: 74.7146 bits (182), Expect = 7.979e-13 Identity = 57/229 (24.89%), Postives = 104/229 (45.41%), Query Frame = -3 Query: 1099 KIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVP------EIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LA---DIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKD 1758 +IF E LK++ I +++ +S G P+M+ G + L+ ++ +V C H + L A+ + + +V+++ + ++N I ++ LN F L + D Y +LL HT ++WL +G L+RF + + F + L+ + I LA D+ N LQG + + I F +K+ L+ + L R +F L +S E D Sbjct: 588 EIFLRVEKSLKKFSIDWSKLVSVASTGTPAMMDANSGLVTKLRARAASCCKGADLKSVRCIIHPEWLCAQKLR--MGHVMDVVVDSVNWICSRGLNHGDFTTLLYELDSQYGSLLYHTALKWLGRGLVLRRFFESLEEIDSFMSSRGKPVPQ--LSSRDWILDLAFLVDMTTHLNTLDASLQGHSQIVTQMYDFIRAFLAKLCLWETHLARNNLAHFPTLKSVSRSESD 812
BLAST of SMED30021434 vs. UniProt/SwissProt
Match: sp|Q8IZ13|ZBED8_HUMAN (Protein ZBED8 OS=Homo sapiens OX=9606 GN=ZBED8 PE=1 SV=1) HSP 1 Score: 110.153 bits (274), Expect = 1.793e-50 Identity = 69/242 (28.51%), Postives = 120/242 (49.59%), Query Frame = -3 Query: 1039 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCI-SSEDITLKTTHLKNLRGF 1755 +F F + ++ I + + +DGA SM+G+ F+AY+K E+P I HC + L+ K + +L + L ++ IN IK +A N RLF ++ Y+ LL HTE+RWLS+G L +++ + +F + + N FK H+ LAD++ N +Q +N + A+ + F K + + ++ F F L ++ V + + I + +ITL HL+ L F Sbjct: 219 VFNLFRDFFLKHKIALDVCGSVCTDGASSMLGENSEFVAYVKKEIPHIVVTHCLLNPHALVIKTLPTKLRDALFTVVRVINFIKGRAPNHRLFQAFFEEIGIEYSVLLFHTEMRWLSRGQILTHIFEMYEEINQFLHHKSSNLVDGFENKEFKIHLAYLADLFKHLNELSASMQRTGMNTVSAREKLSAFVRKFPFWQKRIEKRNFTNFPFLEEIIVSDNEGIFIAAEITL---HLQQLSNF 457 HSP 2 Score: 82.4185 bits (202), Expect = 1.793e-50 Identity = 53/138 (38.41%), Postives = 80/138 (57.97%), Query Frame = -2 Query: 1730 LEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS--KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL-LF*KYF 2131 L+ASY+ AYL AK PHT+ E L+ P E+ VL + + LQ +PLS+ + RID+M+++I Q++ +++ S K I+L E T S LM +VRY + EI+EEF + L+ KG D+ LF +F Sbjct: 92 LQASYQFAYLCAKEKNPHTVAEKLVKPCALEIAQIVLGPDAQKKLQQVPLSDDVIHSRIDEMSQDILQQVLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRY--IKEREIVEEFLFCEPLQLSMKGIDVFNLFRDFF 227 HSP 3 Score: 38.1206 bits (87), Expect = 1.793e-50 Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = -3 Query: 2218 KIRQYSEEYLKFGFVIAPV--N*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQTSK 2406 K R++ ++Y+++ F P C++C FSN ++PS L +H NR H +L + K Sbjct: 3 KKRKWDDDYVRYWFTCTTEVDGTQRPQCVLCNSVFSNADLRPSKLSDHFNRQHGGVAGHDLNSLK 67 HSP 4 Score: 34.6538 bits (78), Expect = 1.793e-50 Identity = 23/81 (28.40%), Postives = 40/81 (49.38%), Query Frame = -1 Query: 759 WIIDPFLCDLTRFDIS--VHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 995 WI+DPFL ++ D S + +L L+ Q + F T FW + +P+L + +L+ F ++Y E FS++ Sbjct: 473 WILDPFLFNIDFVDDSYLMKNDLAELRASGQILMEFETMKLEDFWCAQFT--AFPNLAKTALEILMPFATTYLCELGFSSL 551
BLAST of SMED30021434 vs. UniProt/SwissProt
Match: sp|P0CF97|F200B_HUMAN (Protein FAM200B OS=Homo sapiens OX=9606 GN=FAM200B PE=3 SV=1) HSP 1 Score: 100.138 bits (248), Expect = 1.099e-44 Identity = 65/211 (30.81%), Postives = 105/211 (49.76%), Query Frame = -3 Query: 1120 ILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEV--PEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTL--NRKEFRYFDKLMQ 1734 I+ QY + KN +SDG +M GK+ + L +W HCF HR+ L ++ I + L VL +K +N IK +LNSRL C + + +LL HT++RWLS+G L R ++ + + F E + + + + + T + L DI+ + N KLQG+N ++ I FR + L+ L NR + F + +Q Sbjct: 280 IVGQYKLNWKNCKGITSDGTATMTGKHSRVIKKLLEVTNNGAVWN-HCFIHREGLASREIPQNLMEVLKNAVKVVNFIKGSSLNSRLLETFCSEIGTNHTHLLYHTKIRWLSQGKILSRVYELRNEIHFFLIEKKSHLASIFEDDTWVTKLAYLTDIFSILNELSLKLQGKNSDVFQHVERIQGFRKTLLLWQVRLKSNRPSYYMFPRFLQ 489 HSP 2 Score: 73.559 bits (179), Expect = 1.099e-44 Identity = 53/157 (33.76%), Postives = 89/157 (56.69%), Query Frame = -2 Query: 1748 FLTRSNKVIK---SFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS-KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2203 + R K IK F+ ST+ + E L +SY +AY +AK +T E ++LP +++ T+ + L+ +P +++TV RI +AE++E LIT L++ FAI+LDE T G + L++YVRY +++ +E+F C +L + G DI Sbjct: 121 YFQRKKKDIKLSTQFLSCSTAVS-EKALLSSYLVAYRVAKEKIANTAAEKIILPACLDMVRTIFDDKSADKLKTIP-NDNTVSLRICTIAEHLETMLITRLQSGIDFAIQLDESTDIGSCTTLLVYVRY--AWQDDFLEDFLCFLNLTSHLSGLDIF 273 HSP 3 Score: 52.373 bits (124), Expect = 1.099e-44 Identity = 26/59 (44.07%), Postives = 37/59 (62.71%), Query Frame = -3 Query: 2248 KKKI--RQYSEEYLKFGFVIA--PVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHAD 2412 KKK+ R+Y+E+YLK+GF+ P P C+IC +N+ +KPS LK HL HA+ Sbjct: 55 KKKVSARRYNEDYLKYGFIKCEKPFENDRPQCVICNNILANESLKPSKLKRHLETQHAE 113
BLAST of SMED30021434 vs. UniProt/SwissProt
Match: sp|Q4R6P1|F200A_MACFA (Protein FAM200A OS=Macaca fascicularis OX=9541 GN=FAM200A PE=2 SV=1) HSP 1 Score: 111.694 bits (278), Expect = 1.033e-40 Identity = 77/231 (33.33%), Postives = 119/231 (51.52%), Query Frame = -3 Query: 1093 IFYCFEN-ILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVP--EIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTL--NRKEFRYFDKLMQ---LSVIEKDCI 1755 +F EN I+ QY + K+ SSDGA +M GK+ L +W HCF HR+ L++K I L +VL +K +N IK +LNSRL LC + + +LL HTEVRWLS+G L R ++ + + F E + + N + T + L+DI+G+ N K+QG+N +I I F+ + + + L NR + F L+Q ++I +DC+ Sbjct: 187 LFTELENCIVGQYKLNWKHCKGISSDGAANMTGKHSRLTEKLLEATHNNALWN-HCFIHREALVSKEISPSLMDVLKKAVKIVNFIKGSSLNSRLLEILCSEIGVNHTHLLFHTEVRWLSQGKVLSRVYELRNEIYIFLIEKQSHLANIFENDIWVTKLAYLSDIFGILNELNLKIQGKNNDIFQYLEHILGFQKTLLFWQARLKSNRPSYYMFPTLLQHIEENIINEDCL 416 HSP 2 Score: 80.1073 bits (196), Expect = 1.033e-40 Identity = 53/157 (33.76%), Postives = 89/157 (56.69%), Query Frame = -2 Query: 1748 NFLTRSNKVIKS--FMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS-KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2206 +F + NKV S + ST+ N E L +SY +AY +AK HT E ++LP +++ T+ + L+ +PLS++T+ RRI +A+++E LIT L++ FAI+LDE T L++YVRY +++ +E+ C +L + G D+ Sbjct: 35 HFQKQRNKVESSPQVLSRSTTMN-ERALLSSYLVAYRVAKEKMAHTAAEKIILPACMDMVRTIFDDKSADKLRTIPLSDNTISRRICTIAKHLEAMLITRLQSGIDFAIQLDESTDIASCPTLLVYVRY--VWQDDFVEDLLCCLNLNSHITGLDLF 188
BLAST of SMED30021434 vs. UniProt/SwissProt
Match: sp|Q8TCP9|F200A_HUMAN (Protein FAM200A OS=Homo sapiens OX=9606 GN=FAM200A PE=1 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 5.719e-40 Identity = 75/231 (32.47%), Postives = 118/231 (51.08%), Query Frame = -3 Query: 1093 IFYCFEN-ILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVP--EIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTL--NRKEFRYFDKLMQ---LSVIEKDCI 1755 +F EN +L QY + K+ SSDG +M GK+ L +W HCF HR+ L++K I L +VL +K +N IK +LNSRL C + + +LL HTEVRWLS+G L R ++ + + F E + + + + T + L+DI+G+ N K+QG+N +I I F+ + L+ + L NR + F L+Q ++I +DC+ Sbjct: 187 LFTELENCLLGQYKLNWKHCKGISSDGTANMTGKHSRLTEKLLEATHNNAVWN-HCFIHREALVSKEISPSLMDVLKNAVKTVNFIKGSSLNSRLLEIFCSEIGVNHTHLLFHTEVRWLSQGKVLSRVYELRNEIYIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLKMQGKNNDIFQYLEHILGFQKTLLLWQARLKSNRPSYYMFPTLLQHIEENIINEDCL 416 HSP 2 Score: 79.7221 bits (195), Expect = 5.719e-40 Identity = 53/157 (33.76%), Postives = 88/157 (56.05%), Query Frame = -2 Query: 1748 NFLTRSNKVIKS--FMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS-KFAIKLDE*T-FGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2206 +F NKV S + ST+ N E L +SY +AY +AK HT E ++LP +++ T+ + L+ +PLS++T+ RRI +A+++E LIT L++ FAI+LDE T L++YVRY +++ +E+ C +L + G D+ Sbjct: 35 HFQKERNKVESSPQVLSRSTTMN-ERALLSSYLVAYRVAKEKMAHTAAEKIILPACMDMVRTIFDDKSADKLRTIPLSDNTISRRICTIAKHLEAMLITRLQSGIDFAIQLDESTDIASCPTLLVYVRY--VWQDDFVEDLLCCLNLNSHITGLDLF 188
BLAST of SMED30021434 vs. UniProt/SwissProt
Match: sp|Q6R2W3|SCND3_HUMAN (SCAN domain-containing protein 3 OS=Homo sapiens OX=9606 GN=ZBED9 PE=2 SV=1) HSP 1 Score: 127.872 bits (320), Expect = 4.191e-28 Identity = 71/193 (36.79%), Postives = 108/193 (55.96%), Query Frame = -3 Query: 1231 FFVTSLLKRALKEKIFYCFEN-ILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQL---LNFKTHIF*LADIYGLFNATQNKLQGRN 1797 FF SL +++ +N I+ + + K + SDGA SM GK+ + +K PE T HCF HR+ L K I EL++VLN +K +N IK+ +LNSRLF+ LCD + + LLLH E+RWLS+G L R +I + ++ F + M +QL +N+ + L+DI+ +FN +QG+N Sbjct: 932 FFSASLPTNTTSSELYEAVKNYIVNKCGLEFKFCVGVCSDGAASMTGKHSEVVTQIKELAPECKTTHCFIHRESLAMKKISAELNSVLNDIVKIVNYIKSNSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELL-VFLQGKKPMWSQLFKDVNWTARLAYLSDIFSIFNDLNASMQGKN 1123 HSP 2 Score: 78.9518 bits (193), Expect = 2.874e-20 Identity = 57/157 (36.31%), Postives = 88/157 (56.05%), Query Frame = -2 Query: 1751 FLTRSNKVIKSFMKPSTSKNVE----GGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSK-FAIKLDE*TFGRNSILML-YVRYYSCSKEEIIEEFFCDKSLETCTKGEDI 2203 F R + +KS +P NV L ASYK+A +AK P+TI E L+ I EV +L ++ + +PLSN T+ RRI ++A ++E QLI +++ +K F+++LDE N I++L YVR+ ++I EEFF SL T T ++ Sbjct: 794 FFERKSSELKS--QPKQVFNVSHINISALRASYKVALPVAKSKTPYTIAETLVKDCIKEVCLEMLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVYVRFE--HDDDIKEEFFFSASLPTNTTSSEL 946 HSP 3 Score: 43.8986 bits (102), Expect = 2.874e-20 Identity = 22/53 (41.51%), Postives = 32/53 (60.38%), Query Frame = -3 Query: 2248 RQYSEEYLKFGFVIAPVN*SI--PLCLICEKSFSNDYMKPSILKNHLNRMHAD 2400 R+Y Y++FGFV A ++ + P C+IC +N+ MKPS LK HL H + Sbjct: 735 RKYDPSYIEFGFV-AVIDGEVLKPQCIICGDVLANEAMKPSKLKRHLYSKHKE 786
BLAST of SMED30021434 vs. TrEMBL
Match: A0A5S6QHK7 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 195.282 bits (495), Expect = 7.724e-108 Identity = 93/237 (39.24%), Postives = 157/237 (66.24%), Query Frame = -3 Query: 1045 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNLR 1755 IF ++ L + +IP++NIIA ++DGA SMVG+YRGF+A+LK +P ++T+HC HRQHL++KN+ L++ L++ I+ +N IK+ AL RLF QLC+ +E + LLLHTEVRWLSKG+CL+RF+ +++++V F +D +L+ K IF LADI+ + LQGR+ ++ +K I +F K++++ + R+EF F + +++ + ++ +D+ + HL+ +R Sbjct: 222 IFEEMKSYLAENNIPLENIIACATDGAASMVGRYRGFIAHLKKAIPSVFTIHCVIHRQHLVSKNLGARLNHSLSIVIRTVNFIKSHALRDRLFRQLCEHYNENFRTLLLHTEVRWLSKGNCLRRFVTLWNTIVSFM--SDKEDVGKLIEAKADIFYLADIFEKLDLLNKNLQGRDSTLVSSKEAITSFLKKLEVYWHNMGRREFLQFPNVKEIA----EMVTDDDLLVYVKHLQQIR 452 HSP 2 Score: 119.783 bits (299), Expect = 7.724e-108 Identity = 70/160 (43.75%), Postives = 104/160 (65.00%), Query Frame = -2 Query: 1748 FKNLKCNFLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFGRN-SILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2224 FK L+ NF R V+ + T K ++ G+ ASYKI+ LIAK G+ H + E+L+LP +S +I+ V+N N +Q +PLSNSTV+RRID+MA +IE +L+ LRT KF+++LDE N S+LM YVR+++ K ++EE K ++T T+G I Sbjct: 69 FKALRNNFEKRP--VLTQLLTERTQK-LDKGVVASYKISKLIAKTGHGHNVGESLILPSLSIIISDVMNMNPNSTIQAVPLSNSTVRRRIDEMARDIEERLVDSLRTHKFSLQLDETCLQDNDSLLMAYVRFWNGDK--LMEEMLFAKRIKTDTRGLSIF 223 HSP 3 Score: 68.1662 bits (165), Expect = 7.724e-108 Identity = 31/62 (50.00%), Postives = 42/62 (67.74%), Query Frame = -3 Query: 2227 KKKIRQYSEEYLKFGFVIAPVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQ 2412 KKK R+YS EYL++GF+ +P + S+P+CL C +FSN+ KP LK HL H DKK L+ Sbjct: 6 KKKCRRYSVEYLQYGFIPSPQDQSLPMCLNCMATFSNETTKPCKLKKHLVTSHPDKKDKPLE 67 HSP 4 Score: 66.6254 bits (161), Expect = 7.724e-108 Identity = 35/95 (36.84%), Postives = 50/95 (52.63%), Query Frame = -1 Query: 759 DFNVRFRDMENLCVPAWIIDPFLCDLTRFDISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 1043 D RF D+ NL +P WI++PF+ D T D + E LI L+ D A+ F+ + FW + KYP L ++ + FPS Y VE FS + Sbjct: 454 DMEERFHDLINLNIPDWILNPFVVDPTEVDTEIQESLIDLQSDTSARHQFSLFE-KDFWMKNDLPNKYPTLWEQARTFFVAFPSMYLVECGFSKV 547 HSP 5 Score: 38.1206 bits (87), Expect = 7.724e-108 Identity = 16/28 (57.14%), Postives = 20/28 (71.43%), Query Frame = -3 Query: 631 KGDLRIKLSNFRPNIKEIAQGRQAHLSH 714 +GDLR+ LSN PNI ++AQ QA SH Sbjct: 562 RGDLRLSLSNLEPNIDQLAQKYQAQGSH 589
BLAST of SMED30021434 vs. TrEMBL
Match: A0A5S6QR64 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 209.149 bits (531), Expect = 4.613e-107 Identity = 109/236 (46.19%), Postives = 153/236 (64.83%), Query Frame = -3 Query: 1048 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNL 1755 IF K+ +IP+ NIIA ++DGAPSMVG GF+++LK VP + +HC HRQHL+A+ + E LH L I AIN I+ ++L+ RLF QLC+QNDE +N LLLHT+VRWLSKGSCL RF +F SV+EF + D ++ T L F+ I +AD+Y FN +LQG ++N++ K++I F SK+ F L+RKEF +F L + V +K ++ EDI + HL+ L Sbjct: 221 IFLVVNEFFKEKEIPLTNIIAVATDGAPSMVGCRTGFISHLKKVVPGVLAIHCVIHRQHLVARCLSERLHLSLQYVISAINRIRGRSLSDRLFRQLCEQNDEDFNRLLLHTDVRWLSKGSCLSRFYCLFESVLEFLEKEDPSLCTNLKMFEGDIAYMADLYFKFNKMNLQLQGDDLNLVRTKSVINAFVSKLLFFKENLSRKEFCHFPSLCE--VHKKMQMNEEDIEVYCHHLEML 454 HSP 2 Score: 119.013 bits (297), Expect = 4.613e-107 Identity = 62/139 (44.60%), Postives = 96/139 (69.06%), Query Frame = -2 Query: 1745 STSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TF-GRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDILL 2158 STSK GL+ASY ++ +IAK G PHTI E LLLPV+SE++ TVL++ +L+ +PLSN+TVQRRID+M+ ++E L L+ ++F+++LDE G ++L+ YVR+ +E++++EF + L T +KG I L Sbjct: 87 STSKLQNDGLQASYNVSLMIAKSGKPHTIGEELLLPVVSEILRTVLHKPPAEILKKIPLSNNTVQRRIDEMSRDVEQTLCNFLKNTEFSLQLDESILPGNEALLLAYVRF--IKEEQLVQEFLFARELPTDSKGTSIFL 223 HSP 3 Score: 83.1889 bits (204), Expect = 4.613e-107 Identity = 36/65 (55.38%), Postives = 48/65 (73.85%), Query Frame = -3 Query: 2230 MSKVKKKIRQYSEEYLKFGFVIAPVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNL 2424 MS+ K+K RQYS EYLK+GF+ +P N +P+CL+C K+FSN+ MKPS L HL +MH DK +L Sbjct: 1 MSEKKRKCRQYSTEYLKYGFIASPSNQRLPMCLLCHKTFSNESMKPSRLIEHLAKMHPDKSDKDL 65 HSP 4 Score: 53.1434 bits (126), Expect = 4.613e-107 Identity = 33/98 (33.67%), Postives = 53/98 (54.08%), Query Frame = -1 Query: 759 EDFNVRFRDMENLCVPAWIIDPFLCDLTRFDISVHEELITLK--EDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 1046 +DF RF D+ + +P W+ DPF + + + EEL+ L+ E+++A++ Y +FW + I +P + K LL FPSSY VE FS + Sbjct: 456 KDFVERFEDILAMDIPTWVTDPF-STIENAERHLQEELLELQANEELKAKLKVG---YQRFWLQENIARLHPEIWAVVKKYLLSFPSSYLVERGFSVV 549
BLAST of SMED30021434 vs. TrEMBL
Match: A0A5S6Q2S0 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 191.045 bits (484), Expect = 5.876e-103 Identity = 93/243 (38.27%), Postives = 157/243 (64.61%), Query Frame = -3 Query: 1027 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNLRGF*CPF 1755 IF ++ L + +IP++NIIA ++DGA SMVG+YRGF+A+LK +P ++T+HC HRQHL++KN+ L++ L++ I+ +N IK+ AL RLF QLC+ ++ + LLLHTEVRWLSKG+CL+RF+ +++++V F +D +L+ K IF LADI+ + LQGR+ ++ +K I +F K++++ + R+EF F + +++ + + +D+ + HL+ + PF Sbjct: 447 IFEEMKSYLAENNIPLENIIACATDGAASMVGRYRGFIAHLKKAIPSVFTIHCVIHRQHLVSKNLGARLNHSLSIVIRTVNFIKSHALRDRLFRQLCEHYNDNFRTLLLHTEVRWLSKGNCLRRFVTLWNTIVSFM--SDKEDVGKLIEAKADIFYLADIFEKLDLLNKNLQGRDSTLVSSKEAITSFLKKLEVYWHNMGRREFLQFPNVKEIA----EMVIDDDLLVYVKHLQQIHWILNPF 683 HSP 2 Score: 115.161 bits (287), Expect = 5.876e-103 Identity = 69/160 (43.12%), Postives = 103/160 (64.38%), Query Frame = -2 Query: 1748 FKNLKCNFLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFGRN-SILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2224 FK L+ NF R V+ + T K ++ G+ ASYKI+ LIAK G+ H + E+L+LP +S +I+ V+N +Q +PLSNSTV+RRID+MA +IE +L+ LRT KF+++LDE N S+LM YVR+++ K ++EE K ++T T+G I Sbjct: 294 FKALRNNFEKRP--VLTQLLTERTQK-LDKGVVASYKISKLIAKTGHGHNVGESLILPSLSIIISDVMNMYPNSTIQAVPLSNSTVRRRIDEMALDIEERLVDSLRTHKFSLQLDETCLQDNDSLLMAYVRFWNGDK--LMEEMLFAKRIKTDTRGLSIF 448 HSP 3 Score: 74.3294 bits (181), Expect = 5.876e-103 Identity = 38/81 (46.91%), Postives = 52/81 (64.20%), Query Frame = -3 Query: 2227 FLPLNLFF----YSMSKV-KKKIRQYSEEYLKFGFVIAPVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQ 2454 F P N+F + MS + KKK R+YS EYL++GF+ +P + S+P+CL C +FSN+ MKP LK HL H DKK L+ Sbjct: 212 FTPFNVFIAQPHFPMSSLPKKKCRRYSVEYLQYGFIPSPQDQSLPMCLNCMATFSNETMKPCKLKKHLVTSHPDKKDKPLE 292 HSP 4 Score: 55.0694 bits (131), Expect = 5.876e-103 Identity = 28/79 (35.44%), Postives = 42/79 (53.16%), Query Frame = -1 Query: 759 WIIDPFLCDLTRFDISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 995 WI++PF+ D T D + E LI L+ D A+ F+ + FW + KYP L ++ + FPS+Y VE FS + Sbjct: 678 WILNPFVVDPTEVDTEIQESLIDLQSDTSARHQFSLFE-KDFWMKNDLPNKYPTLWEQARTFFVAFPSTYLVECGFSKV 755 HSP 5 Score: 35.4242 bits (80), Expect = 5.876e-103 Identity = 15/28 (53.57%), Postives = 19/28 (67.86%), Query Frame = -3 Query: 631 KGDLRIKLSNFRPNIKEIAQGRQAHLSH 714 +GDL + LSN PNI ++AQ QA SH Sbjct: 770 RGDLHLSLSNLEPNIDQLAQKYQAQGSH 797
BLAST of SMED30021434 vs. TrEMBL
Match: A0A4Y2IR38 (SCAN domain-containing protein 3 OS=Araneus ventricosus OX=182803 GN=ZBED9_42 PE=4 SV=1) HSP 1 Score: 210.305 bits (534), Expect = 2.593e-102 Identity = 102/234 (43.59%), Postives = 145/234 (61.97%), Query Frame = -3 Query: 1039 ENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNLRGF 1740 E+ L + IP NII+ ++DGAP+M G+YRGF+++LK +P + +HC HRQHL+AKN+ + LH L I A+N I++ ALN+RLFA LCD+NDE + LLLHTEVRWLSKG+CL RF +F SV+EF + L+ +K I L D++ FN +LQG ++N+I K +I F K+ L ++R+EF F L Q+ ++ EDI HL L G+ Sbjct: 180 ESTLPEKSIPFTNIISVAADGAPAMFGQYRGFISHLKRIIPGLIAIHCVIHRQHLVAKNLSDRLHQSLQFVINAVNKIRSNALNTRLFALLCDENDEDFQRLLLHTEVRWLSKGACLSRFYSLFESVIEFLETKTPDLKENLIKYKADIAYLTDLFKKFNDVNLQLQGDSLNLIKTKGVISVFLGKLKLMEQNISRREFSQFPHLSQIECLD------EDIQTYAPHLNTLHGY 407 HSP 2 Score: 102.064 bits (253), Expect = 2.593e-102 Identity = 56/105 (53.33%), Postives = 73/105 (69.52%), Query Frame = -2 Query: 1853 MKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFGRNSI 2167 M STS++ E GL AS I+ LIAK G PHTI E L+LP I EV+ TVL++ VL+ +PLSN+TVQRRID+M+ +IE L L+T+ F+I+LDE T SI Sbjct: 84 MFASTSQSNEDGLRASCNISLLIAKSGKPHTIGEELILPAIEEVLKTVLHKPPFDVLKRIPLSNNTVQRRIDEMSSDIESFLCNYLQTTHFSIQLDESTLPEKSI 188 HSP 3 Score: 73.9442 bits (180), Expect = 2.593e-102 Identity = 33/66 (50.00%), Postives = 47/66 (71.21%), Query Frame = -3 Query: 2227 MSKVKKKIRQYSEEYLKFGFVIAPVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQ 2424 M++ KKK RQYS +YLKFGF+ + + +P+CL+C K SND MKPS L++HL R H +K +L+ Sbjct: 1 MAESKKKCRQYSVDYLKFGFLPSTADKRLPMCLLCNKVLSNDSMKPSKLEDHLRRCHPEKISKDLK 66 HSP 4 Score: 62.003 bits (149), Expect = 2.593e-102 Identity = 36/94 (38.30%), Postives = 51/94 (54.26%), Query Frame = -1 Query: 759 FNVRFRDMENLCVPAWIIDPFLCDLTRFDISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 1040 F RF D+ + +P WII PF + ++ + EEL+ L + + +V F Y FW I EKYP L ++ L+ FPSSY VE FSA+ Sbjct: 408 FKNRFEDILTMDIPPWIITPF-DETEEANVVLQEELLELSTNEELKVKFKK-GYQTFWLQAEIPEKYPGLWEIARKFLIAFPSSYLVERSFSAV 499
BLAST of SMED30021434 vs. TrEMBL
Match: G1KVP8 (Uncharacterized protein OS=Anolis carolinensis OX=28377 PE=4 SV=1) HSP 1 Score: 207.223 bits (526), Expect = 6.705e-100 Identity = 105/224 (46.88%), Postives = 147/224 (65.62%), Query Frame = -3 Query: 1090 EKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCIS 1761 E IF+ + K+ +IP+ NI++ ++DGAP MVG Y FLAYLK +VP +TVHC HRQHL+AKN+ LHN L I+AIN+I++K+LN RLF QLC +NDE +N LLLHTEVRWLSKG+CL RF +F+SV+EF ++ + + L+ K+ I L D++ FN + +LQG +N+I K +I F +K+ L+ R EF F L +KD +S Sbjct: 205 ESIFHALDEFFKEKEIPLSNILSLATDGAPVMVGCYSDFLAYLK-KVPNAFTVHCIIHRQHLVAKNLSARLHNSLQYVIQAINTIRSKSLNDRLFKQLCIENDEEFNRLLLHTEVRWLSKGACLDRFYKLFNSVLEFLKGKNDALQSNLIQSKSDIAYLTDLFQKFNESNLQLQGDELNLIKTKPVISAFVTKL-LYKRNFGRGEFSQFPNLSGAEKKDKDILS 426 HSP 2 Score: 100.908 bits (250), Expect = 6.705e-100 Identity = 69/167 (41.32%), Postives = 100/167 (59.88%), Query Frame = -2 Query: 1748 KIQQFTNFKNLKCNFLTRSNKVIKSFMKPSTSKNVEG-GLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2245 K++ + F+ LK F+ + + K F ST+ +G GL ASYKI +IAK G PHTI E ++P ISEVI VL++ +++ + LSN+TVQRR+D+MA+++E L L+TS F+I+LDE T N L+L + + II K L+T TKGE I Sbjct: 55 KVKDLSYFRTLKEKFIEQPT-LAKLF---STTTTQDGDGLLASYKILLMIAKLGKPHTIGEEQIIPAISEVIHIVLHKPASDIIKKISLSNNTVQRRMDEMAQDVEDSLCGYLKTSWFSIQLDESTLLGNKTLLL-----AYGSQRII----IAKYLQTDTKGESIF 208 HSP 3 Score: 76.6406 bits (187), Expect = 6.705e-100 Identity = 32/59 (54.24%), Postives = 45/59 (76.27%), Query Frame = -3 Query: 2230 KIRQYSEEYLKFGFVIAPVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNL 2406 K RQY+ EYLK+GF+ +PVN ++P+CLIC+K S + MKPS L+ HL ++H DKK +L Sbjct: 1 KCRQYNIEYLKYGFIESPVNNTLPMCLICQKVLSKEAMKPSRLQEHLTKIHGDKKVKDL 59 HSP 4 Score: 45.4394 bits (106), Expect = 6.705e-100 Identity = 30/94 (31.91%), Postives = 46/94 (48.94%), Query Frame = -1 Query: 759 FNVRFRDMENLCVPAWIIDPFLCDLTRFDISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 1040 FN RF D+ + +P F C +H+EL+ + + + + F + Y +FW K I YP + + L+ FPSSY VE FSA+ Sbjct: 438 FNQRFEDILKMNIPGSF---FKCK--EESPQLHDELMEVTTNDELKFKFKS-GYQQFWLQKEIPTAYPEIWTVIQKFLIAFPSSYLVERGFSAV 525 HSP 5 Score: 30.8018 bits (68), Expect = 6.705e-100 Identity = 14/41 (34.15%), Postives = 20/41 (48.78%), Query Frame = -3 Query: 631 FIKKNKEQTQYY*KGDLRIKLSNFRPNIKEIAQGRQAHLSH 753 + K + Q Q +GDLR+ L P I ++ Q H SH Sbjct: 528 LLTKKRNQLQIVNRGDLRLLLMKIEPRITKLVAAYQVHPSH 568
BLAST of SMED30021434 vs. Ensembl Cavefish
Match: ENSAMXT00000057314.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:4224188:4227217:-1 gene:ENSAMXG00000029268.1 transcript:ENSAMXT00000057314.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 134.806 bits (338), Expect = 3.130e-53 Identity = 76/216 (35.19%), Postives = 114/216 (52.78%), Query Frame = -3 Query: 1114 EKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLS 1761 E IF + + ++IP KNI+A +DGA SM+GK +GF + LK + P HC HR L K++ E+L+ L +K +N IKA+ +N R+FAQLC+ DE + LLLHTEVRWLS+G L KT LADI+ L+N ++QG + +I+ K + F K++ + L + + ++F L+Q S Sbjct: 217 EDIFSAVDTYITSHNIPYKNIVACCTDGAASMMGKNKGFNSRLKEKAPHCLVFHCMVHRHALAGKHLCEDLNETLKTVVKIVNFIKARPVNRRIFAQLCE--DETHQTLLLHTEVRWLSRGKFL---------------------------IKTAY--LADIFSLYNEANKRMQGADGTVIECKETVDAFVRKVEFRRNKLKKGDLQHFPLLLQQS 401 HSP 2 Score: 79.7221 bits (195), Expect = 3.130e-53 Identity = 47/127 (37.01%), Postives = 77/127 (60.63%), Query Frame = -2 Query: 1748 ASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE-*TFGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2125 AS++ + LIAK PHTI E L+ P ++ + + +PLSN+TV+ RID+MA N+E L+ +L+TS F+I+LDE T +IL++Y +Y + E+ ++ +L++ T+GEDI Sbjct: 96 ASFECSLLIAKDKKPHTIGETLIKPACIKMAERMCGPQVANKFKIVPLSNNTVKDRIDRMAGNVENTLVEKLKTSPFSIQLDETTTVAEEAILIVYAQYIEGT--EVKQDILMSVNLKSTTRGEDIF 220 HSP 3 Score: 37.3502 bits (85), Expect = 3.130e-53 Identity = 27/100 (27.00%), Postives = 48/100 (48.00%), Query Frame = -1 Query: 759 EDFNVRFRDMENLCVP-AWIIDPFLCDLTRFD-ISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLL--LIFPSSYFVEACFSAI 1046 E+ + RF D+ L AW++DPFL + + +EL+ +K + ++ H + +FW K P L ++ + L F ++Y E FSA+ Sbjct: 422 EEMHSRFSDIHELVSKDAWVMDPFLAKEDDVEHLQAEDELMDIKSNSVCMRFYDEHGFKQFWLAKGP-GVAPRLANHADHQISGLPFATTYLSETAFSAL 520
BLAST of SMED30021434 vs. Ensembl Cavefish
Match: ENSAMXT00000051422.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:35461191:35465166:-1 gene:ENSAMXG00000032672.1 transcript:ENSAMXT00000051422.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 114.39 bits (285), Expect = 5.881e-52 Identity = 62/193 (32.12%), Postives = 105/193 (54.40%), Query Frame = -3 Query: 1126 IAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFK--THIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKL 1698 + S+DGA +M+G+ G +A +K P +VHC HR+ L AK + +L VL+ +K +N IK++ L SRLF LC++ + LLLHTEVRWLS+G L R ++ V F +++ ++ + +F+ + L+DI+ N LQG+++ + I K+D++ + + + FD L Sbjct: 315 VGVSTDGARAMMGQRSGVIARVKAVAPLATSVHCSLHREALAAKRMSRDLKIVLDEAVKIVNFIKSRPLQSRLFRVLCEEMGSSHVQLLLHTEVRWLSRGKVLARLFELRDEVRIFLLDSNFELSDRFTDFEWLAKLSYLSDIFTHLNGLNISLQGKSVTAFHVQNKIEATIKKLDMWTKRVEQSNYESFDNL 507 HSP 2 Score: 88.1965 bits (217), Expect = 5.881e-52 Identity = 55/125 (44.00%), Postives = 79/125 (63.20%), Query Frame = -2 Query: 1766 GLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKF-AIKLDE*TFGRN-SILMLYVRYYSCSKEEIIEEFFCDKSLETCT 2134 +EASY++A LIAK G PHTI E+L+LP E+++ +L + L + LS++TV+RRI++MA ++ QLI +R S+F AI+LDE T N S L+ +VRY EI E+ K L T T Sbjct: 169 AVEASYRVAQLIAKAGKPHTIGESLILPAAKEMVSVMLGEKPCKQLNLISLSDNTVERRINEMAHDVTQQLIKRVRESRFYAIQLDESTDIANLSNLLAFVRYE--HNREIFEDILFCKPLPTKT 291 HSP 3 Score: 44.669 bits (104), Expect = 5.881e-52 Identity = 18/50 (36.00%), Postives = 29/50 (58.00%), Query Frame = -3 Query: 2248 YSEEYLKFGFVIAPV-N*SIPLCLICEKSFSNDYMKPSILKNHLNRMHAD 2394 Y EYL GF N +PLC++C + +N+ +KP+ L+ HL H++ Sbjct: 85 YCSEYLALGFTCTGTENEPLPLCVVCSEVLANEALKPTKLRRHLETKHSE 134
BLAST of SMED30021434 vs. Ensembl Cavefish
Match: ENSAMXT00000039226.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000099.1:564912:568121:1 gene:ENSAMXG00000042173.1 transcript:ENSAMXT00000039226.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 123.25 bits (308), Expect = 1.688e-30 Identity = 76/254 (29.92%), Postives = 131/254 (51.57%), Query Frame = -3 Query: 1054 SLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEV--PEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTM-----TTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYF---DKLMQLSVIEKDCISSEDITLKTTHLK 1785 L +R E I+ F + D+P + +++ + DGAP+M G + GF+A + + P + HC H+Q L +K + + +V+ + +K +NSI+AKAL RLF L D+ D Y +L+LH +VRWLS+G LQRFI++ ++ F T LL+ L D+ N +LQG++ ++ + + F+ K+ L+ S L K+ +F DK++ ++ KD E+ + L+ Sbjct: 215 PLNERTRGEDIYRAFRTYATENDLPFQKLVSITRDGAPAMCGIHAGFIALCRKDPIFPAFVSYHCVIHQQALASKVV--DFSHVMTVVVKIVNSIRAKALQHRLFKSLLDELDAEYGDLILHADVRWLSRGKVLQRFINLLPEIISFLKSRKEEYRELADDTWLLDLAF----LTDLTSKLNELNCELQGKDCDVPHMVSAVNAFKVKLALWTSQLKCKKLTHFQNVDKMLN-NISNKDSFHPENFCIHLNRLE 461 HSP 2 Score: 31.187 bits (69), Expect = 1.688e-30 Identity = 20/61 (32.79%), Postives = 30/61 (49.18%), Query Frame = -1 Query: 768 SVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACF 950 V E+I L+ D++ + D FW + V RE+YP L + + F S+YF E F Sbjct: 492 GVDMEIIDLENDIEMKARSRDKD---FWGL-VSRERYPSLSACALKVKAFFGSTYFCEMSF 548
BLAST of SMED30021434 vs. Ensembl Cavefish
Match: ENSAMXT00000037725.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:29162452:29166863:1 gene:ENSAMXG00000037303.1 transcript:ENSAMXT00000037725.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 106.301 bits (264), Expect = 8.121e-30 Identity = 63/226 (27.88%), Postives = 118/226 (52.21%), Query Frame = -3 Query: 1135 VTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLK-----NEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFE--NDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYF 1791 + L R +IF F+ ++ ++P + A ++DGA S+VG+ GF+ +LK ++ E+ HC H++ L K++H +++ + A+N I+A++L R F + Y +++ HTEVRWLS+G+ L+RF + + ++ F E +T+ + ++ + L D+ GL N KLQG++ I + F K++LF + L + +F Sbjct: 400 LVPLYGRTTGFEIFNAFKTAVENLNLPWSKLSAVTTDGALSVVGETNGFIGHLKRHLGPDQAAELKHYHCIIHQEALCGKHLH--FKEIMDFVVSAVNFIRARSLKHREFPSFLEDISAAYGDVIYHTEVRWLSRGNVLKRFFALRNE-IKLFLEQKGKDTLVMENTDWIADLAFLTDLTGLLNELNLKLQGKDRLICHLFEAVCAFEMKLNLFATQLKKGNLTHF 622 HSP 2 Score: 34.2686 bits (77), Expect = 8.121e-30 Identity = 30/107 (28.04%), Postives = 52/107 (48.60%), Query Frame = -2 Query: 1835 KNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELR-TSKFAIKLDE*TFGRNSI-LMLYVR 2149 K E ASY+IA L+AK P I + + E + + + +N+ LS +T+Q RI ++ N QL + + + F+ LDE T ++ L +++R Sbjct: 281 KESEACTIASYRIANLVAKSARPF-IDGEFVKDCMLEACDVICPEA-KDAFKNISLSRTTIQLRIVDLSSNSYCQLQSRINDVTYFSAALDESTDNTDTAQLSIFIR 385 HSP 3 Score: 31.9574 bits (71), Expect = 8.121e-30 Identity = 24/93 (25.81%), Postives = 41/93 (44.09%), Query Frame = -1 Query: 765 FNVRFRDMENLCVPAWII-DPFLCDLTRFDISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFS 1040 F+ RF N ++ DPF D + E+I LK + +F+ + RE++P+L N++ + +F S+Y E FS Sbjct: 650 FSARFGQFRNEQATLQLLADPFSVDTETVPGELQPEIIELKCSTAMRTKHREMPLLEFYQ-SLDREQFPNLFANAQKWISMFGSTYICEQMFS 741
BLAST of SMED30021434 vs. Ensembl Cavefish
Match: ENSAMXT00000030525.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:12992682:12994541:1 gene:ENSAMXG00000035573.1 transcript:ENSAMXT00000030525.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 103.219 bits (256), Expect = 9.198e-29 Identity = 66/221 (29.86%), Postives = 117/221 (52.94%), Query Frame = -3 Query: 1135 VTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFK--THIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYF 1791 V + + ++IF+ + ++ +P K + ++DGAPSM G+ G + V E +HC H+Q L +K + + NV+++ +K IN I++++L R F L ++ + Y ++L TEVRWLS+G+ L+RF ++ + V+ F E D T L + K + L DI N KLQG+ + A + F +K+ L+ + L++ F +F Sbjct: 267 VIPMHGQTTAQEIFHQLCDAIENAGLPWKRFVGITTDGAPSMTGRKNGLE---EESVEEAIALHCIIHQQALCSKCL--KFDNVMSVVVKCINQIRSRSLKHRRFRALLEEMESEYGDVLYFTEVRWLSRGNILKRFFELRAE-VKAFMEKDGVAVTVLSDPKWLMDLAFLVDITHELNVLNKKLQGQGQLVSAAYDNVRAFSAKLMLWKAQLSQTNFCHF 481 HSP 2 Score: 45.4394 bits (106), Expect = 9.198e-29 Identity = 35/116 (30.17%), Postives = 61/116 (52.59%), Query Frame = -2 Query: 1835 KSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITEL-RTSKFAIKLDE*T-FGRNSILMLYVR 2176 + F K +T +N + +EASY ++ +IAK G P T E + ++ +++ + N+ LS +TV RI ++ +I QL + R S +++ LDE T + L +YVR Sbjct: 140 QDFFKKATKEN-DAAVEASYVVSEMIAKAGKPFTEGEFVKKCILQ--AASIVCPEKKGQFSNISLSANTVAERISDLSSDIYDQLCEKAKRFSVYSVALDESTDITDTAQLAIYVR 252
BLAST of SMED30021434 vs. Ensembl Nematostella
Match: EDO43282 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZ18]) HSP 1 Score: 57.3806 bits (137), Expect = 4.569e-8 Identity = 49/229 (21.40%), Postives = 91/229 (39.74%), Query Frame = -3 Query: 1222 YAIIV----VQRKK*SRSFFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAI---------NSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLL----------NFKTHIF*LADIYGLFNATQNKLQGRNINI 1839 Y ++V V R + + F+ L+ + IF ++ + Y++P +N+I S D A +GK+ G + + + ++T C H H A + + NS K +A + + F CDQ+ Y +L + RWLS+ C+ R + + S+ +F + + L + H+ I +F+ +LQG I Sbjct: 142 YPLVVRIYDVNRGEVASQFWHMCLISDCTAQGIFNSVSDVFRDYEVPWENVIGLSLDNASVNMGKHNGLYRHFERKNASVYTFGCPCHIVHNTASYGAKAYAEAAGFDLGDFLVDIYYYFDNSTKRQA-SLKDFCDFCDQD---YRKILKYGATRWLSRELCVSRVLKQYPSLQSYFASQPELRSDKRLCRLREYFADPXTEIHLMFFQSILPVFSELNKQLQGEEPQI 366
BLAST of SMED30021434 vs. Ensembl Medaka
Match: ENSORLT00000032459.1 (pep primary_assembly:ASM223467v1:8:14240678:14247355:1 gene:ENSORLG00000024991.1 transcript:ENSORLT00000032459.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 139.428 bits (350), Expect = 5.805e-56 Identity = 81/251 (32.27%), Postives = 126/251 (50.20%), Query Frame = -3 Query: 1051 FFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKN 1797 F L RA E+ F C +N + + +N + SDGA SM G++RG + + + PE HCF HR++L AK + E H V+++ +K IN IK+ A+NSR FAQLC+ + + LL H+EVRWLS+G L R ++ + + F + + + N F + L+DI+ L N LQGRN NI + F+ K+ L+ + + F L + S ++ +HL N Sbjct: 210 LFCGELPTRATAEECFRCMDNYFTENGLDWQNCVGVCSDGAASMTGRHRGVIKQILDLAPEAKWTHCFLHRENL-AKKMSPEFHEVMDVTVKTINFIKSNAVNSRCFAQLCEGMEADHVQLLYHSEVRWLSRGLVLTRIFELRNEIFSFLTDKKSPLAHHYTNATFIAKLAYLSDIFSLLNQLNISLQGRNSNIFFVADKVQAFQKKLSLWSKRAHEERLDMFPLLSDILESSPQVKISHSVSQHLSHLAN 459 HSP 2 Score: 64.3142 bits (155), Expect = 5.805e-56 Identity = 44/126 (34.92%), Postives = 71/126 (56.35%), Query Frame = -2 Query: 1790 STSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSK-FAIKLDE*TFGRNSILML-YVRY-YSCSKEEIIEEFFC 2158 S + + + L+ASY +A +A+ TI E L+LP ++ ++ + L+ +PLSN V RI +A +IE QL+ +R S F+++LDE T N+ L+L +VRY + S +E I FC Sbjct: 89 SLTGDAKCALKASYLVARRVAQSKKAFTIAEELVLPAAVDMCREMIGEAAAKKLKTIPLSNDIVSHRIADIASDIEQQLLERIRKSPFFSLQLDESTDVTNAALLLVFVRYRWDSSLQEDI--LFC 212 HSP 3 Score: 41.5874 bits (96), Expect = 5.805e-56 Identity = 23/83 (27.71%), Postives = 43/83 (51.81%), Query Frame = -1 Query: 759 WIIDPFLCDLTRFDIS----VHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 995 W++DPF D T D++ + +L+ + D + + D FW V+ ++YP L + + +LL F ++Y E+ FS + Sbjct: 475 WVVDPFSVDPTENDVALPSHLESQLLEVSTDSTLKGQWGKLDLGSFWI--VVSKEYPLLALRAFKVLLPFTTTYLCESGFSTV 555 HSP 4 Score: 36.1946 bits (82), Expect = 5.805e-56 Identity = 22/59 (37.29%), Postives = 31/59 (52.54%), Query Frame = -3 Query: 2251 SKVKKKIRQYSEEYLKFGFVIAPVN*SIPL--CLICEKSFSNDYMKPSILKNHLNRMHA 2421 S K IR+Y+ + + FGFV + P C+ C + SN+ +KPS LK HL H Sbjct: 6 SSKAKFIRKYNPDLINFGFVNGGSE-AEPRAQCVECGLTLSNEALKPSKLKRHLETKHP 63
BLAST of SMED30021434 vs. Ensembl Medaka
Match: ENSORLT00000035512.1 (pep primary_assembly:ASM223467v1:11:22712371:22714941:1 gene:ENSORLG00000026495.1 transcript:ENSORLT00000035512.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 112.079 bits (279), Expect = 1.404e-51 Identity = 97/409 (23.72%), Postives = 175/409 (42.79%), Query Frame = -3 Query: 631 FFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLL---NFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNLRGF*CPFSRYGKFMCPSLDH*SIPL*FN*V*YFGT*RTNYVEGGCPSSSII*HT*LCKILDHQSDS*KVPAFVN----------E*QTPIVN---FSVILFCRSLFQCNQ*FIK----KNKEQTQYY*KGDLRIKLSNFRPNIKEIAQGRQAHLSH 1797 F + RA E++F + L + + +N I SDGA +M G +G A +K P + HC HR+ L ++ + EL V++ + +N IK + L +R+F+ +C++ + +L H+E RWLS+G L R ++ + F + + NF + L+DI+G N +LQG++ ++ I +F K+ ++ L+ F+ L + V D ++ I H+ +L GF F +Y D P FN P+ + + +D SDS F + E Q P++ ++L + + C F K K ++Q + +LR+ +S F+P +++ ++AH SH Sbjct: 226 LFCKYVRDRATAEELFKMLDCFLTENGLKWENCIGVCSDGAQTMAGMKKGLRALIKKASPNVEWTHCVIHREALASRKLSSELSEVMSDIVGVVNFIKTRPLKTRVFSAICEEMGAEHQAVLFHSEARWLSRGKVLSRVFELREQIRMFLEQEHKHEVAEKFSDENFLAKLAYLSDIFGKLNELNLQLQGKDKHLPQVTDKISSFTRKLAMWGRRLDEGNTDSFENLHEF-VATTDYDATSVIPHIKEHISSLIGF---FKKYFPENSSQYDWVRDP--FN----------------APAPTSFSSAEEDQFIDMTSDSTLRLRFTSQTLSEFWLNVERQYPLLGQRAMGILLPFATSYLCETGFSAVAALKTKYRSQLNIEQELRVAVSCFKPRFEKLCTAKRAHCSH 612 HSP 2 Score: 83.5741 bits (205), Expect = 1.404e-51 Identity = 45/132 (34.09%), Postives = 81/132 (61.36%), Query Frame = -2 Query: 1832 FKNLKCNFLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFG-RNSILMLYVRY 2224 F+ + + +S++ + + TS N + L ASYK+AY IA+ PHTI E L+LP ++++ +L+ L+ +PLSN TV RRI+ +A +++ QL+ +L+ +FA++ DE T ++ + + YVR+ Sbjct: 90 FQRKRAEYCQQSSRFVSA-----TSVN-QRALLASYKVAYQIAQCKKPHTIAEELILPAALDMVSVMLDDASAAKLKTVPLSNDTVARRINDIANDLKEQLVEKLKDKRFALQFDEATDSNKDCLFIAYVRF 215 HSP 3 Score: 50.447 bits (119), Expect = 1.404e-51 Identity = 26/64 (40.62%), Postives = 38/64 (59.38%), Query Frame = -3 Query: 2245 MSKVKKKIRQYSEEYLKFGFVIAPV-N*SIPLCLICEKSFSNDYMKPSILKNHLNRM---HADK 2424 +S K K+R+Y E Y+ GF + V + P+CL+C K + D MKP+ L+ HL + HADK Sbjct: 22 VSLPKAKMRKYDEAYVALGFTVTTVGDEERPVCLLCLKMLAADSMKPNKLRRHLKTLHPNHADK 85
BLAST of SMED30021434 vs. Ensembl Medaka
Match: ENSORLT00000040389.1 (pep primary_assembly:ASM223467v1:19:265767:268275:-1 gene:ENSORLG00000025045.1 transcript:ENSORLT00000040389.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 112.079 bits (279), Expect = 2.171e-51 Identity = 97/409 (23.72%), Postives = 175/409 (42.79%), Query Frame = -3 Query: 631 FFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLL---NFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNLRGF*CPFSRYGKFMCPSLDH*SIPL*FN*V*YFGT*RTNYVEGGCPSSSII*HT*LCKILDHQSDS*KVPAFVN----------E*QTPIVN---FSVILFCRSLFQCNQ*FIK----KNKEQTQYY*KGDLRIKLSNFRPNIKEIAQGRQAHLSH 1797 F + RA E++F + L + + +N I SDGA +M G +G A +K P + HC HR+ L ++ + EL V++ + +N IK + L +R+F+ +C++ + +L H+E RWLS+G L R ++ + F + + NF + L+DI+G N +LQG++ ++ I +F K+ ++ L+ F+ L + V D ++ I H+ +L GF F +Y D P FN P+ + + +D SDS F + E Q P++ ++L + + C F K K ++Q + +LR+ +S F+P +++ ++AH SH Sbjct: 226 LFCKYVRDRATAEELFKMLDCFLTENGLKWENCIGVCSDGAQTMAGMKKGLRALIKKASPNVEWTHCVIHREALASRKLSSELSEVMSDIVGVVNFIKTRPLKTRVFSAICEEMGAEHQAVLFHSEARWLSRGKVLSRVFELREQIRMFLEQEHKHEVAEKFSDENFLAKLAYLSDIFGKLNELNLQLQGKDKHLPQVTDKISSFTRKLAMWGRRLDEGNTDSFENLHEF-VATTDYDATSVIPHIKEHISSLIGF---FKKYFPENSSQYDWVRDP--FN----------------APAPTSFSSAEEDQFIDMTSDSTLRLRFTSQTLSEFWLNVERQYPLLGQRAMGILLPFATSYLCETGFSAVAALKTKYRSQLNIEQELRVAVSCFKPRFEKLCTAKRAHCSH 612 HSP 2 Score: 83.5741 bits (205), Expect = 2.171e-51 Identity = 45/132 (34.09%), Postives = 81/132 (61.36%), Query Frame = -2 Query: 1832 FKNLKCNFLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*T-FGRNSILMLYVRY 2224 F+ + + +S++ + + TS N + L ASYK+AY IA+ PHTI E L+LP ++++ +L+ L+ +PLSN TV RRI+ +A +++ QL+ +L+ +FA++ DE T ++ + + YVR+ Sbjct: 90 FQRKRAEYCQQSSRFVSA-----TSVN-QRALLASYKVAYQIAQCKKPHTIAEELILPAALDMVSVMLDDASAAKLKTVPLSNDTVARRINDIANDLKEQLVEKLKDKRFALQFDEATDSNKDCLFIAYVRF 215 HSP 3 Score: 49.6766 bits (117), Expect = 2.171e-51 Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = -3 Query: 2245 MSKVKKKIRQYSEEYLKFGFVIAPV-N*SIPLCLICEKSFSNDYMKPSILKNHLNRM---HADK 2424 +S K K R+Y E Y+ GF + V + P+CL+C K + D MKP+ L+ HL + HADK Sbjct: 22 VSLPKAKTRKYDEAYVALGFTVTTVGDEERPVCLLCLKMLAADSMKPNKLRRHLKTLHPNHADK 85
BLAST of SMED30021434 vs. Ensembl Medaka
Match: ENSORLT00000028620.1 (pep primary_assembly:ASM223467v1:3:35179252:35181570:1 gene:ENSORLG00000028714.1 transcript:ENSORLT00000028620.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 137.887 bits (346), Expect = 2.209e-50 Identity = 73/211 (34.60%), Postives = 111/211 (52.61%), Query Frame = -3 Query: 1171 FFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN--FKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLF 1797 F L RA E+ F C +N + + ++ + SDGA SM G++RG + + + PE HCF HR++L AK + E H +++ +K IN IK+ A+NSR FAQLC+ + + LL H+EVRWLS+G R ++ V F + + + N F + L+DI+ L N LQGRN NI + F+ K+ L+ Sbjct: 256 LFCGELPTRATAEECFRCMDNYFTENGLDWQSCVGVCSDGAASMTGRHRGVIKQILDLAPEAKWTHCFLHRENLAAKKMSPEFHEAMDVTVKTINFIKSNAVNSRCFAQLCEGMEADHVKLLYHSEVRWLSRGLVFTRIFELRKEVFSFLTDKKSPLAHHYTNATFIAKLAYLSDIFSLLNQLNISLQGRNSNIFFVADKVPAFQRKLSLW 466 HSP 2 Score: 64.6994 bits (156), Expect = 2.209e-50 Identity = 45/126 (35.71%), Postives = 70/126 (55.56%), Query Frame = -2 Query: 1790 STSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSK-FAIKLDE*TFGRNSILML-YVRY-YSCSKEEIIEEFFC 2158 S + + + L+ASY +A +A TI E L+LP ++ ++ + L+ +PLSN V RI MA +IE QL+ +R S F+++LDE T N+ L+L +VRY + S +E I FC Sbjct: 135 SLTGDAKCALKASYLVARRVALNKKAFTIAEELVLPAAVDMCREMIGEAAAKKLKTIPLSNDIVSHRIADMASDIEQQLLGRIRKSPFFSLQLDESTDVTNAALLLVFVRYRWDSSLQEDI--LFC 258 HSP 3 Score: 39.6614 bits (91), Expect = 2.209e-50 Identity = 26/70 (37.14%), Postives = 38/70 (54.29%), Query Frame = -3 Query: 2230 FYSMSKVKKKIRQYSEEYLKFGFVIAPVN*SIPL--CLICEKSFSNDYMKPSILKNHLNRMHADKKYSNL 2433 + S S K IR+Y+ +++KFGFV + P C+ C + SN+ +KPS LK HL + KY L Sbjct: 48 YASPSSKAKFIRKYNPDFIKFGFVNGGSE-AEPRAQCVECGLTLSNEALKPSKLKRHL-----ETKYPKL 111
BLAST of SMED30021434 vs. Ensembl Medaka
Match: ENSORLT00000032191.1 (pep primary_assembly:ASM223467v1:7:34400406:34401509:1 gene:ENSORLG00000024356.1 transcript:ENSORLT00000032191.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 78.9518 bits (193), Expect = 2.297e-38 Identity = 54/132 (40.91%), Postives = 78/132 (59.09%), Query Frame = -2 Query: 1751 LEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQN-DRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTS--KFAIKLDE*TFGRN-SILMLYVRYYSCSKEEII-EEFFCDKSLETCTKGEDI 2131 L ASY++AYLIAK G PHTI E L+ P ++ +L L +PLSN T+ RI M+ +I Q++ +L +S KF+++LDE T N S L ++VRY K+++I EEF K L T +K D+ Sbjct: 95 LTASYEVAYLIAKQGEPHTIGETLVKPAALKMANLILGTTAAEGKLSQIPLSNDTISDRIGDMSNDILAQVVADLISSPAKFSLQLDETTDVANLSQLAVFVRYV---KDDMIKEEFLFCKPLTTTSKAADV 223 HSP 2 Score: 72.4034 bits (176), Expect = 2.297e-38 Identity = 37/126 (29.37%), Postives = 62/126 (49.21%), Query Frame = -3 Query: 1309 SDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLN 1686 +DGAP M+G+ GF A + P I HC HR L K + ++ VL ++ + ++ L R+F++LC + + L+ H+ VRWLS+ SC + + F T++ +LL Sbjct: 246 TDGAPVMLGRKSGFGALVNAGAPHIVVTHCILHRHALATKTLPPKMAEVLKTVVECVIYVRNSTLRHRIFSELCKEMGAEFEVLMYHSNVRWLSQ-SCF-----CLACGISLVFGRAPTLSCRLLQ 365 HSP 3 Score: 50.447 bits (119), Expect = 2.297e-38 Identity = 23/63 (36.51%), Postives = 40/63 (63.49%), Query Frame = -3 Query: 2233 VKKKIRQYSEEYLKFGF-VIAPVN*SI-PLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSN 2415 + K R++S+EY+++GF I ++ S P+C+IC SN + P+ LK H+ ++H D +Y N Sbjct: 1 MSSKKRKWSDEYVQYGFSCITELDGSQRPICMICNAKLSNSNLAPAKLKEHIEKLHGDGEYKN 63
BLAST of SMED30021434 vs. Planmine SMEST
Match: SMESG000077232.1 (SMESG000077232.1) HSP 1 Score: 444.506 bits (1142), Expect = 1.691e-141 Identity = 216/254 (85.04%), Postives = 229/254 (90.16%), Query Frame = -3 Query: 1045 SRSFFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNLR 1806 R+ + SL E IFYCFENILKQYDIPIKNIIAASSDGAP++VGKYRGFLAYLKNEVPEIW VHCF HRQHL+AKNIHEEL+N+LNLCIKAINSI+AKALNSRLFAQLCDQNDEVYNNLLLHTE RWLSK +CLQRFIDIFSSVVEFF ENDNTM QLLNFKTHIF LADIYGLFNATQNKL GRNIN+IDAKTIIF+FRSKIDLFISTLNRKEFRYF LMQ+SVIEKDCISSEDITL T LKNL+ Sbjct: 187 GRNSILMSLKTYTKGEDIFYCFENILKQYDIPIKNIIAASSDGAPTIVGKYRGFLAYLKNEVPEIWKVHCFLHRQHLVAKNIHEELYNILNLCIKAINSIRAKALNSRLFAQLCDQNDEVYNNLLLHTEGRWLSKSNCLQRFIDIFSSVVEFFLENDNTMAMQLLNFKTHIFYLADIYGLFNATQNKLLGRNINLIDAKTIIFSFRSKIDLFISTLNRKEFRYFGNLMQISVIEKDCISSEDITLYTNRLKNLK 440 HSP 2 Score: 224.942 bits (572), Expect = 1.408e-85 Identity = 121/159 (76.10%), Postives = 129/159 (81.13%), Query Frame = -2 Query: 1748 FKNLKCNFLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDIL 2224 FKNLK NFLTRSNKVIKSFMKPSTSKNV GGL+ASYKIAYLIAKGG PHTI ENLLLPVISEVITTVLNQND+PVLQ+LPL NSTVQ+RID+MAENIELQLITELRTSKFAI+L + TFGRNSILM SL+T TKGEDI Sbjct: 68 FKNLKSNFLTRSNKVIKSFMKPSTSKNVLGGLKASYKIAYLIAKGGCPHTIGENLLLPVISEVITTVLNQNDKPVLQSLPLLNSTVQQRIDEMAENIELQLITELRTSKFAIQLGKSTFGRNSILM---------------------SLKTYTKGEDIF 205 HSP 3 Score: 113.62 bits (283), Expect = 1.408e-85 Identity = 54/66 (81.82%), Postives = 58/66 (87.88%), Query Frame = -3 Query: 2227 MSKVKKKIRQYSEEYLKFGFVIAPVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQ 2424 MS KKKI QYSEEY KFGFV+AP N S+PLCLICEKSFSNDYMKPS LK+HL+RMHADKK SNLQ Sbjct: 1 MSTEKKKICQYSEEYSKFGFVVAPCNSSMPLCLICEKSFSNDYMKPSKLKDHLDRMHADKKDSNLQ 66
BLAST of SMED30021434 vs. Planmine SMEST
Match: SMESG000029634.1 (SMESG000029634.1) HSP 1 Score: 425.631 bits (1093), Expect = 8.716e-137 Identity = 214/250 (85.60%), Postives = 223/250 (89.20%), Query Frame = -3 Query: 1048 FFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISSEDITLKTTHLKNL 1797 F V SL E IF+CFENILKQYDIPIK I SSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCF HRQHL+AKNIHEELHNVLNL IKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEV WLSKG+CLQRFIDIFS VVEFF ENDNTM QLLNFKTHIF LADIYGLFNATQNKLQ RNIN++DAKTIIF+FRS I+LFISTLNRKEFRYF+ LMQLSVIEKDC SS+DI L TTHLKNL Sbjct: 19 FCVKSLETYTKGENIFFCFENILKQYDIPIKKI-PPSSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFLHRQHLVAKNIHEELHNVLNLLIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVLWLSKGNCLQRFIDIFSLVVEFFLENDNTMAMQLLNFKTHIFYLADIYGLFNATQNKLQRRNINLVDAKTIIFSFRSNINLFISTLNRKEFRYFENLMQLSVIEKDCNSSQDIILYTTHLKNL 267 HSP 2 Score: 72.0182 bits (175), Expect = 5.212e-13 Identity = 31/32 (96.88%), Postives = 32/32 (100.00%), Query Frame = -1 Query: 951 EDFNVRFRDMENLCVPAWIIDPFLCDLTRFDI 1046 EDFNVRFRDMENLCVPAWII+PFLCDLTRFDI Sbjct: 269 EDFNVRFRDMENLCVPAWIIEPFLCDLTRFDI 300 HSP 3 Score: 62.3882 bits (150), Expect = 8.672e-10 Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = -2 Query: 1751 MLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDI 1849 MLYVRYYSCSKEEIIEEFFC KSLET TKGE+I Sbjct: 1 MLYVRYYSCSKEEIIEEFFCVKSLETYTKGENI 33
BLAST of SMED30021434 vs. Planmine SMEST
Match: SMESG000063081.1 (SMESG000063081.1) HSP 1 Score: 352.058 bits (902), Expect = 1.398e-108 Identity = 166/191 (86.91%), Postives = 179/191 (93.72%), Query Frame = -3 Query: 1183 IFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSK 1755 IFYCFENILKQYDIPI+NIIAASSDGAPSMV KYRGFLAYLKN+VPE+WTVHCF HRQHL+AKNIHEELHNVLNLCIKAINSI+ KALNSRLFAQLCDQNDEVYNNLLLHT+VR++SKG+CLQRFIDI SVVEFF ENDNTM QLLNFKTHIF LADIYGLFNATQ KLQGRNIN+IDA+T+IF+F K Sbjct: 206 IFYCFENILKQYDIPIQNIIAASSDGAPSMVAKYRGFLAYLKNKVPEMWTVHCFLHRQHLVAKNIHEELHNVLNLCIKAINSIRVKALNSRLFAQLCDQNDEVYNNLLLHTKVRFISKGNCLQRFIDILGSVVEFFLENDNTMAMQLLNFKTHIFYLADIYGLFNATQIKLQGRNINLIDAQTVIFSFDLK 396 HSP 2 Score: 113.235 bits (282), Expect = 4.065e-26 Identity = 55/61 (90.16%), Postives = 55/61 (90.16%), Query Frame = -2 Query: 2042 FKNLKCNFLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVIS 2224 FKNLKCNFLTRSNK K FMKPSTSKNVEGGLEASYKIAY IAKGGY HTI ENLLLPVIS Sbjct: 123 FKNLKCNFLTRSNKEFKFFMKPSTSKNVEGGLEASYKIAYFIAKGGYLHTIGENLLLPVIS 183
BLAST of SMED30021434 vs. Planmine SMEST
Match: SMESG000010447.1 (SMESG000010447.1) HSP 1 Score: 281.567 bits (719), Expect = 2.242e-100 Identity = 147/179 (82.12%), Postives = 154/179 (86.03%), Query Frame = -2 Query: 1676 FLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGEDILLF*KYFKAI*YSYK---KHYCRIF*WS 2203 FLTRS KVIKSFMKPSTSKNVEGGLE SYKIAYLIAKGGYPHTI ENLLLPVISEVIT VLNQND+PVLQ+LPLSNSTVQRRID+MAENIELQL TELRT+KFAI+LDE TFGRN ILMLYVRYYSCSKEE+IEEFFC KSLET TKGEDI F+ I Y KHYCR F WS Sbjct: 68 FLTRSYKVIKSFMKPSTSKNVEGGLETSYKIAYLIAKGGYPHTIGENLLLPVISEVITNVLNQNDKPVLQSLPLSNSTVQRRIDEMAENIELQLNTELRTTKFAIQLDESTFGRNCILMLYVRYYSCSKEEMIEEFFCVKSLETYTKGEDIFYC---FENILKQYDIHIKHYCRFFCWS 243 HSP 2 Score: 106.301 bits (264), Expect = 2.242e-100 Identity = 52/66 (78.79%), Postives = 57/66 (86.36%), Query Frame = -3 Query: 2227 MSKVKKKIRQYSEEYLKFGFVIAPVN*SIPLCLICEKSFSNDYMKPSILKNHLNRMHADKKYSNLQ 2424 MS KKKIRQ+SEEYLKFGFV+AP N S+ LCLIC+K FSNDYMK S LK+HLNRMHADKK SNLQ Sbjct: 1 MSTEKKKIRQFSEEYLKFGFVVAPGNSSMSLCLICQKRFSNDYMKLSKLKDHLNRMHADKKDSNLQ 66 HSP 3 Score: 85.1149 bits (209), Expect = 1.217e-17 Identity = 46/66 (69.70%), Postives = 47/66 (71.21%), Query Frame = -3 Query: 1603 FFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAA-SSDGAPSMVGKYRGFLAYLKNEVPEIWTV 1797 F V SL E IFYCFENILKQYDI IK+ APSMVGKYRGFLAYLKNEVPEIWTV Sbjct: 204 FCVKSLETYTKGEDIFYCFENILKQYDIHIKHYCRFFCWSLAPSMVGKYRGFLAYLKNEVPEIWTV 269
BLAST of SMED30021434 vs. Planmine SMEST
Match: SMESG000042414.1 (SMESG000042414.1) HSP 1 Score: 288.115 bits (736), Expect = 8.069e-85 Identity = 143/157 (91.08%), Postives = 150/157 (95.54%), Query Frame = -2 Query: 1754 FKNLKCNFLTRSNKVIKSFMKPSTSKNVEGGLEASYKIAYLIAKGGYPHTIRENLLLPVISEVITTVLNQNDRPVLQNLPLSNSTVQRRIDKMAENIELQLITELRTSKFAIKLDE*TFGRNSILMLYVRYYSCSKEEIIEEFFCDKSLETCTKGED 2224 FKNLKCNFLTRSNKVIKSFMKPSTSK+VEGGLEASYKIAYLIAKGGY HTI ENLLLPVISE+ITTVLNQND+P+LQ+LPLSNSTVQRRI +MAENIELQLITELRTSKFAI+LDE TF RNSILMLYVRYYSCSKEEIIEEFFC KSLET TKGED Sbjct: 25 FKNLKCNFLTRSNKVIKSFMKPSTSKDVEGGLEASYKIAYLIAKGGYMHTIGENLLLPVISEIITTVLNQNDKPILQSLPLSNSTVQRRIGEMAENIELQLITELRTSKFAIQLDESTFDRNSILMLYVRYYSCSKEEIIEEFFCAKSLETYTKGED 181 HSP 2 Score: 253.832 bits (647), Expect = 4.662e-73 Identity = 174/414 (42.03%), Postives = 213/414 (51.45%), Query Frame = -3 Query: 631 FFVTSLLKRALKEKIFYCFENILKQYDIPIKNIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAINSIKAKALNSRLFAQLCDQNDEVYNNLLLHTEVRWLSKGSCLQRFIDIFSSVVEFFFENDNTMTTQLLNFKTHIF*LADIYGLFNATQNKLQGRNINIIDAKTIIFNFRSKIDLFISTLNRKEFRYFDKLMQLSVIEKDCISS--------------EDITLKTTHLKNL-------RGF*CPFSRYGKFMCPSL----DH*SIPL*FN*V*YFGT*RTNYVEGGCPSSSII*HT*LCKILDHQSDS*KVPAFVNE*QTPIVNFSVILFCRSLFQCNQ*FIKKNKEQTQYY*KGDLRIKLSNFRPNIKEIAQGRQAHLSH 1797 F SL E FYCFENILKQYDIPI+NIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCF H Q+L+AKNIHE+LHNVLNLCIKAINSI+AKALNSRL V + K + + FI ++ ++ T+ + LL F H FRSKIDLFISTLNRKEFRYFD LM LSVIEKDCISS ED ++ ++NL F C +R+ + L + + FN G SS+I K++ ++ P + ++ F F + F ++K + + +GDLRIKLSN++PNIKEIAQ QAHLSH Sbjct: 168 FCAKSLETYTKGEDTFYCFENILKQYDIPIENIIAASSDGAPSMVGKYRGFLAYLKNEVPEIWTVHCFLHIQYLVAKNIHEKLHNVLNLCIKAINSIRAKALNSRLLPNC----------------VTKMMKCTTICFFIRKYAGSLK------ATVCSDLLIFLVH----------------------------------FRSKIDLFISTLNRKEFRYFDNLMHLSVIEKDCISSEDITLYTTHLKNLKEDFNVRYRDMENLCVPARIIEPFLCDLTRFDISVQEELITLKEGVQAQVLFN------------TYGYAKSSTI-------KVIREKN-----PHLQMNSEPLLLTFPSSYFVEAGFSAINNLLRKTRNKLNIIERGDLRIKLSNYKPNIKEIAQEHQAHLSH 501 HSP 3 Score: 168.318 bits (425), Expect = 8.647e-44 Identity = 82/96 (85.42%), Postives = 86/96 (89.58%), Query Frame = -1 Query: 759 EDFNVRFRDMENLCVPAWIIDPFLCDLTRFDISVHEELITLKEDVQAQVLFNTHDYAKFWTIKVIREKYPHL*MNSKPLLLIFPSSYFVEACFSAI 1046 EDFNVR+RDMENLCVPA II+PFLCDLTRFDISV EELITLKE VQAQVLFNT+ YAK TIKVIREK PHL MNS+PLLL FPSSYFVEA FSAI Sbjct: 363 EDFNVRYRDMENLCVPARIIEPFLCDLTRFDISVQEELITLKEGVQAQVLFNTYGYAKSSTIKVIREKNPHLQMNSEPLLLTFPSSYFVEAGFSAI 458 HSP 4 Score: 78.5666 bits (192), Expect = 1.797e-14 Identity = 36/43 (83.72%), Postives = 36/43 (83.72%), Query Frame = -1 Query: 1353 FLPNCVTKMMKCTTICFFIPKYAGSLKAAVCSDLLIFLAQLLS 1481 LPNCVTKMMKCTTICFFI KYAGSLKA VCSDLLIFL S Sbjct: 273 LLPNCVTKMMKCTTICFFIRKYAGSLKATVCSDLLIFLVHFRS 315 The following BLAST results are available for this feature:
BLAST of SMED30021434 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30021434 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30021434 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30021434 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of SMED30021434 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of SMED30021434 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of SMED30021434 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30021434 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30021434 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30021434 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30021434 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30021434 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30021434 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30021434 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021434 ID=SMED30021434|Name=SMED30021434|organism=Schmidtea mediterranea sexual|type=transcript|length=4904bpback to top protein sequence of SMED30021434-orf-1 >SMED30021434-orf-1 ID=SMED30021434-orf-1|Name=SMED30021434-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp MWDAGFTVSQVREARDNSLQQAEDFELPFVTIKREPGRNPAREIRCQAEFback to top protein sequence of SMED30021434-orf-2 >SMED30021434-orf-2 ID=SMED30021434-orf-2|Name=SMED30021434-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=127bp MVGKYRGFLAYLKNEVPEIWTVHCFWHRQHLIAKNIHEELHNVLNLCIKAback to top Annotated Terms
The following terms have been associated with this transcript:
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