Kunitz-type serine protease inhibitor

Overview
NameKunitz-type serine protease inhibitor
Smed IDSMED30021397
Length (bp)310
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Kunitz-type serine protease inhibitor (SMED30021397) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Kunitz-type serine protease inhibitor (SMED30021397) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021397

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30021397 SmedASXL_067230SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30021397 dd_Smed_v4_12691_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30021397 dd_Smed_v4_12691_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30021397 dd_Smed_v4_12691_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 63.5438 bits (153), Expect = 1.332e-12
Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            M+A +      C  P   GPCK Y PR+ +N   G+C  F++GGC+ NGNNF +++ CE  C
Sbjct:  282 MLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 343
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 63.5438 bits (153), Expect = 1.543e-12
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+   GKCVRFI+GGC  N NNF ++  C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 63.5438 bits (153), Expect = 1.545e-12
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+   GKCVRFI+GGC  N NNF ++  C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 63.5438 bits (153), Expect = 1.545e-12
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+   GKCVRFI+GGC  N NNF ++  C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 62.7734 bits (151), Expect = 2.251e-12
Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            M+A +      C  P   GPCK Y PR+ +N   G+C  F++GGC+ NGNNF +++ CE  C
Sbjct:  375 MLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 436
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 58.151 bits (139), Expect = 3.952e-11
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P++ GPCK +  R+YFN   G C  F +GGC  N N+F T+K CE+HC +
Sbjct: 1074 CLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHCAR 1126

HSP 2 Score: 51.9878 bits (123), Expect = 5.660e-9
Identity = 25/64 (39.06%), Postives = 35/64 (54.69%), Query Frame = 2 
Query:   65 LVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +V + T  ++   C  P + GPC  Y  RFYFN  + +C  F +GGC+ N NNF   + CE  C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 3 Score: 51.2174 bits (121), Expect = 1.320e-8
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 2 
Query:   95 RYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            R  C  P  +G C  Y PR++FN   G+C +F +G C  N NNF  +  CE  C
Sbjct: 2165 RVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2218

HSP 4 Score: 50.8322 bits (120), Expect = 1.573e-8
Identity = 25/62 (40.32%), Postives = 32/62 (51.61%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +MA  KS    C+  ++ G C G F RF F+  I  C  F +GGC  N NNF T + C   C
Sbjct:  846 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 907

HSP 5 Score: 50.8322 bits (120), Expect = 1.803e-8
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 2 
Query:  125 GPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            GPC G F R+++N    KC +F + GC  NGNN+ +++ CE  C
Sbjct:  430 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 6 Score: 48.9062 bits (115), Expect = 8.229e-8
Identity = 22/53 (41.51%), Postives = 30/53 (56.60%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P  SG C+G F R++F+     C  F + GC+ NGNNF +K+ C   C K
Sbjct:  728 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAICHK 780
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 58.151 bits (139), Expect = 4.030e-11
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P++ GPCK +  R+YFN   G C  F +GGC  N N+F T+K CE+HC +
Sbjct: 1083 CLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHCAR 1135

HSP 2 Score: 51.9878 bits (123), Expect = 5.660e-9
Identity = 25/64 (39.06%), Postives = 35/64 (54.69%), Query Frame = 2 
Query:   65 LVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +V + T  ++   C  P + GPC  Y  RFYFN  + +C  F +GGC+ N NNF   + CE  C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 3 Score: 51.2174 bits (121), Expect = 1.346e-8
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 2 
Query:   95 RYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            R  C  P  +G C  Y PR++FN   G+C +F +G C  N NNF  +  CE  C
Sbjct: 2174 RVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2227

HSP 4 Score: 50.8322 bits (120), Expect = 1.573e-8
Identity = 25/62 (40.32%), Postives = 32/62 (51.61%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +MA  KS    C+  ++ G C G F RF F+  I  C  F +GGC  N NNF T + C   C
Sbjct:  855 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 916

HSP 5 Score: 50.8322 bits (120), Expect = 1.820e-8
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 2 
Query:  125 GPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            GPC G F R+++N    KC +F + GC  NGNN+ +++ CE  C
Sbjct:  430 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 6 Score: 48.9062 bits (115), Expect = 8.391e-8
Identity = 22/53 (41.51%), Postives = 30/53 (56.60%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P  SG C+G F R++F+     C  F + GC+ NGNNF +K+ C   C K
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAICHK 789
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 58.151 bits (139), Expect = 4.571e-11
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P++ GPCK +  R+YFN   G C  F +GGC  N N+F T+K CE+HC +
Sbjct:  954 CLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHCAR 1006

HSP 2 Score: 51.9878 bits (123), Expect = 6.420e-9
Identity = 25/64 (39.06%), Postives = 35/64 (54.69%), Query Frame = 2 
Query:   65 LVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +V + T  ++   C  P + GPC  Y  RFYFN  + +C  F +GGC+ N NNF   + CE  C
Sbjct:  397 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 460

HSP 3 Score: 50.8322 bits (120), Expect = 1.512e-8
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 2 
Query:   95 RYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            R  C  P  +G C  Y PR++FN   G+C +F +G C  N NNF  +  CE  C
Sbjct: 2045 RVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2098

HSP 4 Score: 50.8322 bits (120), Expect = 1.767e-8
Identity = 25/62 (40.32%), Postives = 32/62 (51.61%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +MA  KS    C+  ++ G C G F RF F+  I  C  F +GGC  N NNF T + C   C
Sbjct:  726 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 787

HSP 5 Score: 50.447 bits (119), Expect = 2.085e-8
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 2 
Query:  125 GPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            GPC G F R+++N    KC +F + GC  NGNN+ +++ CE  C
Sbjct:  301 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 344

HSP 6 Score: 48.521 bits (114), Expect = 9.424e-8
Identity = 22/53 (41.51%), Postives = 30/53 (56.60%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P  SG C+G F R++F+     C  F + GC+ NGNNF +K+ C   C K
Sbjct:  608 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAICHK 660
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 57.7658 bits (138), Expect = 6.225e-11
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P++ GPCK +  R+YFN   G C  F +GGC  N N+F T+K CE+HC +
Sbjct: 1083 CLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHCAR 1135

HSP 2 Score: 51.6026 bits (122), Expect = 8.575e-9
Identity = 25/64 (39.06%), Postives = 35/64 (54.69%), Query Frame = 2 
Query:   65 LVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +V + T  ++   C  P + GPC  Y  RFYFN  + +C  F +GGC+ N NNF   + CE  C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 3 Score: 50.447 bits (119), Expect = 2.000e-8
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 2 
Query:   95 RYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            R  C  P  +G C  Y PR++FN   G+C +F +G C  N NNF  +  CE  C
Sbjct: 1648 RVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1701

HSP 4 Score: 50.447 bits (119), Expect = 2.314e-8
Identity = 25/62 (40.32%), Postives = 32/62 (51.61%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +MA  KS    C+  ++ G C G F RF F+  I  C  F +GGC  N NNF T + C   C
Sbjct:  855 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 916

HSP 5 Score: 50.0618 bits (118), Expect = 2.839e-8
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 2 
Query:  125 GPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            GPC G F R+++N    KC +F + GC  NGNN+ +++ CE  C
Sbjct:  430 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 6 Score: 48.1358 bits (113), Expect = 1.271e-7
Identity = 22/53 (41.51%), Postives = 30/53 (56.60%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P  SG C+G F R++F+     C  F + GC+ NGNNF +K+ C   C K
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAICHK 789
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3f.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 57.3806 bits (137), Expect = 7.623e-11
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P++ GPCK +  R+YFN   G C  F +GGC  N N+F T+K CE+HC +
Sbjct:  862 CLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHCAR 914

HSP 2 Score: 51.2174 bits (121), Expect = 1.000e-8
Identity = 25/64 (39.06%), Postives = 35/64 (54.69%), Query Frame = 2 
Query:   65 LVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +V + T  ++   C  P + GPC  Y  RFYFN  + +C  F +GGC+ N NNF   + CE  C
Sbjct:  305 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 368

HSP 3 Score: 50.447 bits (119), Expect = 2.356e-8
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 2 
Query:   95 RYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            R  C  P  +G C  Y PR++FN   G+C +F +G C  N NNF  +  CE  C
Sbjct: 1427 RVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1480

HSP 4 Score: 50.0618 bits (118), Expect = 2.699e-8
Identity = 25/62 (40.32%), Postives = 32/62 (51.61%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +MA  KS    C+  ++ G C G F RF F+  I  C  F +GGC  N NNF T + C   C
Sbjct:  634 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKC 695

HSP 5 Score: 50.0618 bits (118), Expect = 3.216e-8
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 2 
Query:  125 GPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            GPC G F R+++N    KC +F + GC  NGNN+ +++ CE  C
Sbjct:  209 GPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 252

HSP 6 Score: 48.1358 bits (113), Expect = 1.399e-7
Identity = 22/53 (41.51%), Postives = 30/53 (56.60%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C  P  SG C+G F R++F+     C  F + GC+ NGNNF +K+ C   C K
Sbjct:  516 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAICHK 568
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: CG17380 (gene:FBgn0039077 transcript:FBtr0299867)

HSP 1 Score: 53.5286 bits (127), Expect = 3.092e-10
Identity = 30/84 (35.71%), Postives = 44/84 (52.38%), Query Frame = 2 
Query:   56 FVFLVMMATIKSQRYN-CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKRHQF*MKNKYLII 304
             +FL++  T  + R+  C      GPCKG F  F ++     CV FI+GGC  N N F+TKK C + C     +    ++YLI+
Sbjct:    8 LIFLIVPGTSTALRHERCSFIANPGPCKGNFEMFAYDMDNNVCVEFIYGGCGGNPNRFQTKKECILLCNALADE----DEYLIV 87
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0300337)

HSP 1 Score: 55.4546 bits (132), Expect = 4.408e-10
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C+ P++SGPC G F R+Y+++    C  F +GGCK N NN+ T+  C  +CR+
Sbjct: 1730 CEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 45.0542 bits (105), Expect = 2.476e-6
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C  P ++G C  Y  ++YF+   G C RF +GGC  N N F ++  C+  C+
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQ 1663
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301838)

HSP 1 Score: 55.0694 bits (131), Expect = 5.990e-10
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C+ P++SGPC G F R+Y+++    C  F +GGCK N NN+ T+  C  +CR+
Sbjct: 1730 CEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 44.669 bits (104), Expect = 3.112e-6
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C  P ++G C  Y  ++YF+   G C RF +GGC  N N F ++  C+  C+
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQ 1663
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301839)

HSP 1 Score: 55.0694 bits (131), Expect = 6.230e-10
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C+ P++SGPC G F R+Y+++    C  F +GGCK N NN+ T+  C  +CR+
Sbjct: 1730 CEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 44.669 bits (104), Expect = 3.237e-6
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C  P ++G C  Y  ++YF+   G C RF +GGC  N N F ++  C+  C+
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQ 1663

HSP 3 Score: 44.2838 bits (103), Expect = 3.499e-6
Identity = 23/62 (37.10%), Postives = 34/62 (54.84%), Query Frame = 2 
Query:   83 IKSQRYNCKDPMKSGPCKG---YFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            + S +  C  P+ +G C G   +  R+Y++   G CV FI+ GC  N NNFR+ + C   CR
Sbjct: 2254 VGSAKEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCR 2315
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301837)

HSP 1 Score: 55.0694 bits (131), Expect = 7.142e-10
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C+ P++SGPC G F R+Y+++    C  F +GGCK N NN+ T+  C  +CR+
Sbjct: 1730 CEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 44.2838 bits (103), Expect = 3.466e-6
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C  P ++G C  Y  ++YF+   G C RF +GGC  N N F ++  C+  C+
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQ 1663

HSP 3 Score: 44.2838 bits (103), Expect = 3.858e-6
Identity = 23/62 (37.10%), Postives = 34/62 (54.84%), Query Frame = 2 
Query:   83 IKSQRYNCKDPMKSGPCKG---YFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            + S +  C  P+ +G C G   +  R+Y++   G CV FI+ GC  N NNFR+ + C   CR
Sbjct: 2311 VGSAKEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCR 2372
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:ZFIN;Acc:ZDB-GENE-061009-28])

HSP 1 Score: 60.8474 bits (146), Expect = 8.361e-12
Identity = 23/61 (37.70%), Postives = 38/61 (62.30%), Query Frame = 2 
Query:   80 TIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            T++     C    ++GPC+   PR++F+    KCVRFI+GGC  N NNF +++ C + C++
Sbjct:  307 TLQEVEAVCSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMVVCKR 367
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:ZFIN;Acc:ZDB-GENE-061009-28])

HSP 1 Score: 60.4622 bits (145), Expect = 9.592e-12
Identity = 23/61 (37.70%), Postives = 38/61 (62.30%), Query Frame = 2 
Query:   80 TIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            T++     C    ++GPC+   PR++F+    KCVRFI+GGC  N NNF +++ C + C++
Sbjct:  307 TLQEVEAVCSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMVVCKR 367
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 a [Source:ZFIN;Acc:ZDB-GENE-040426-2169])

HSP 1 Score: 60.077 bits (144), Expect = 1.362e-11
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 2 
Query:   68 VMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            +++ T +   ++C  P K GPC+G FPR+++N    KC  F FGGC PN NN+     C+  C K
Sbjct:  227 ILVLTREQSLHHCLVPKKEGPCRGSFPRWHYNAATEKCEEFKFGGCVPNRNNYLALNECQSACNK 291
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 a [Source:ZFIN;Acc:ZDB-GENE-040426-2169])

HSP 1 Score: 60.077 bits (144), Expect = 1.367e-11
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 2 
Query:   68 VMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            +++ T +   ++C  P K GPC+G FPR+++N    KC  F FGGC PN NN+     C+  C K
Sbjct:  227 ILVLTREQSLHHCLVPKKEGPCRGSFPRWHYNAATEKCEEFKFGGCVPNRNNYLALNECQSACNK 291
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 a [Source:ZFIN;Acc:ZDB-GENE-040426-2169])

HSP 1 Score: 60.077 bits (144), Expect = 1.367e-11
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 2 
Query:   68 VMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            +++ T +   ++C  P K GPC+G FPR+++N    KC  F FGGC PN NN+     C+  C K
Sbjct:  227 ILVLTREQSLHHCLVPKKEGPCRGSFPRWHYNAATEKCEEFKFGGCVPNRNNYLALNECQSACNK 291
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 60.077 bits (144), Expect = 2.370e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+    KCVRFI+GGC  N NNF ++  C   C+
Sbjct:  275 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 326
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 60.077 bits (144), Expect = 2.371e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+    KCVRFI+GGC  N NNF ++  C   C+
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 60.077 bits (144), Expect = 2.372e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+    KCVRFI+GGC  N NNF ++  C   C+
Sbjct:  292 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 343
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 60.077 bits (144), Expect = 2.373e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+    KCVRFI+GGC  N NNF ++  C   C+
Sbjct:  300 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 351
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 60.077 bits (144), Expect = 2.373e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+    KCVRFI+GGC  N NNF ++  C   C+
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 63.5438 bits (153), Expect = 1.059e-12
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+   GKCVRFI+GGC  N NNF ++  C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 62.7734 bits (151), Expect = 1.891e-12
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            C     +GPC+   PR+YF+   GKCVRFI+GGC  N NNF ++  C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Wfdc6a (WAP four-disulfide core domain 6A [Source:MGI Symbol;Acc:MGI:2684968])

HSP 1 Score: 58.9214 bits (141), Expect = 4.589e-12
Identity = 23/55 (41.82%), Postives = 34/55 (61.82%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKR 268
            C  P   GPC  Y PR+++N     C  FI+GGC+ N NNF ++ +C + C+KK+
Sbjct:   77 CSLPQDPGPCLAYLPRWWYNQETDLCTEFIYGGCQGNPNNFPSEGICTVVCKKKQ 131
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Wfdc6a (WAP four-disulfide core domain 6A [Source:MGI Symbol;Acc:MGI:2684968])

HSP 1 Score: 58.9214 bits (141), Expect = 4.589e-12
Identity = 23/55 (41.82%), Postives = 34/55 (61.82%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKR 268
            C  P   GPC  Y PR+++N     C  FI+GGC+ N NNF ++ +C + C+KK+
Sbjct:   77 CSLPQDPGPCLAYLPRWWYNQETDLCTEFIYGGCQGNPNNFPSEGICTVVCKKKQ 131
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Wfikkn2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:MGI Symbol;Acc:MGI:2669209])

HSP 1 Score: 61.2326 bits (147), Expect = 6.089e-12
Identity = 26/62 (41.94%), Postives = 36/62 (58.06%), Query Frame = 2 
Query:   71 MMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            M+A +      C  P   GPCK Y PR+ +N   G C  F++GGC+ NGNNF +++ CE  C
Sbjct:  370 MLACMSGPLAICSLPALQGPCKAYVPRWAYNSQTGLCQSFVYGGCEGNGNNFESREACEESC 431
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DMJ5|VKT_HETCR (PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 2.981e-15
Identity = 33/78 (42.31%), Postives = 42/78 (53.85%), Query Frame = 2 
Query:   32 MKFLITFCFVFLVMMATIKSQRYN-CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            MK     C + +   +   +Q  + C +P   GPC  YFPRFYF+   GKC  FI+GGC+ NGNNF T   C   CR 
Sbjct:    1 MKGTFLICLILIAGFSFKSTQAGSICLEPKVVGPCTAYFPRFYFDSETGKCTPFIYGGCEGNGNNFETLHACRAICRA 78
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|C0HJF3|VKT3_HETCR (TauPI-stichotoxin-Hcr2d OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.669e-14
Identity = 30/53 (56.60%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C +P   GPC  YFPRFYFN   GKC  FI+GGC+ NGNNF T + C   CR 
Sbjct:    4 CLEPKVVGPCTAYFPRFYFNSETGKCTPFIYGGCEGNGNNFETLRACRGICRA 56
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|B2G331|VKT1_HETCR (TauPI-stichotoxin-Hcr2b OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.605e-14
Identity = 32/78 (41.03%), Postives = 42/78 (53.85%), Query Frame = 2 
Query:   32 MKFLITFCFVFLVMMATIKSQRYN-CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            MK     C + +   +   +Q  + C +P   GPC  YF RFYF+   GKC  FI+GGC+ NGNNF T + C   CR 
Sbjct:    1 MKGTFLICLILIAGFSFKSTQAGSICLEPKVVGPCTAYFRRFYFDSETGKCTVFIYGGCEGNGNNFETLRACRAICRA 78
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|C0HK74|VKT3_HETMG (Kunitz-type serine protease inhibitor HMGS2 OS=Heteractis magnifica OX=38281 PE=1 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.907e-14
Identity = 29/53 (54.72%), Postives = 33/53 (62.26%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C +P   GPC  YFPRFYF+   GKC  FI+GGC+ NGNNF T   C   CR 
Sbjct:    4 CLEPKVVGPCTAYFPRFYFDSETGKCTPFIYGGCEGNGNNFETLHACRAICRA 56
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|P81902|CSTI_BOMMO (Trypsin inhibitor OS=Bombyx mori OX=7091 PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 5.352e-14
Identity = 27/52 (51.92%), Postives = 36/52 (69.23%), Query Frame = 2 
Query:  101 NCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +C  P+K+GPCKG FPR+ ++    KCV FI+GGC+ N NNF T + CE  C
Sbjct:    3 DCLLPIKTGPCKGSFPRYAYDSSEDKCVEFIYGGCQANANNFETIEECEAAC 54
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A3M6TIL3 (BPTI/Kunitz inhibitor domain-containing protein OS=Pocillopora damicornis OX=46731 GN=pdam_00003238 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.331e-14
Identity = 34/79 (43.04%), Postives = 47/79 (59.49%), Query Frame = 2 
Query:   20 LLIEMKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            + IE   L  F ++FL     I +Q   C+ PM +G C+   PR+Y+N    KC +FIFGGCK N NNF+TK  C+ +C
Sbjct:    4 IAIEPVLLSLFVWIFLSFGRAIPNQSSICRQPMDTGLCRQALPRWYYNATTRKCEKFIFGGCKGNNNNFKTKDDCKRNC 82
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A4Y2DL81 (Carboxypeptidase inhibitor SmCI OS=Araneus ventricosus OX=182803 GN=PCPI_1 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.696e-14
Identity = 38/87 (43.68%), Postives = 52/87 (59.77%), Query Frame = 2 
Query:   32 MKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKRHQF*MKNK 292
            MKFL+   FV +   A+  S R  C  P ++G C+GYFPRFYF+   G+C  F++GGC  N NNFRT+  CE  C  +  Q   +N+
Sbjct:    1 MKFLVILAFV-VGAFASEGSDR--CDLPAETGLCRGYFPRFYFDKATGQCKNFVYGGCGGNENNFRTEDECESACGNRAAQVCGENE 84

HSP 2 Score: 55.8398 bits (133), Expect = 4.228e-7
Identity = 26/54 (48.15%), Postives = 33/54 (61.11%), Query Frame = 2 
Query:   95 RYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            R  C++  + GPCK  F RFYFN   G+C  FI+GGC+ N NNF +   CE  C
Sbjct:  146 RNACEEDKEVGPCKAAFARFYFNKKTGQCEPFIYGGCEGNSNNFLSLDDCEDVC 199
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: I1ZII0 (Serine protease inhibitor-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.738e-14
Identity = 33/73 (45.21%), Postives = 44/73 (60.27%), Query Frame = 2 
Query:   44 ITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            I F  + + +   I      C  P  +GPCK YFPR+Y+N   GKC +FI+GGCK N NNF++ K CE +C K
Sbjct:    3 ILFILLCICVFENISCLNSICSLPKNAGPCKAYFPRYYYNSKTGKCEKFIYGGCKGNQNNFQSSKDCEQNCHK 75
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: Q8T7L9 (Kazal-type serine proteinase inhibitor 1 OS=Bombyx mori OX=7091 GN=SPI1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 7.381e-14
Identity = 28/70 (40.00%), Postives = 45/70 (64.29%), Query Frame = 2 
Query:   47 TFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +  F+ ++++ +  +   +C  P+K+GPCKG FPR+ ++    KCV FI+GGC+ N NNF T + CE  C
Sbjct:    6 SISFLLIILLFSFTNANPDCLLPIKTGPCKGSFPRYAYDSSEDKCVEFIYGGCQANANNFETIEECEAAC 75
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A151HYY2 (Taicatoxin, serine protease inhibitor component OS=Atta colombica OX=520822 GN=ALC53_12607 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.147e-13
Identity = 30/81 (37.04%), Postives = 49/81 (60.49%), Query Frame = 2 
Query:   26 IEMKFLITFCFVFLVMMATIKSQRYN---CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            + +K  + F F+ +++M  I S++     C  PM +GPC+   PR+ +N   G+C +FI+GGC  N NNF+T   CE+ C+
Sbjct:    5 MNLKARLLFVFIIVILMHEITSKQLYPSLCLLPMNTGPCRAQIPRYAYNSASGQCEQFIYGGCHANANNFKTISECEMTCK 85
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:103032340])

HSP 1 Score: 61.6178 bits (148), Expect = 3.964e-12
Identity = 27/65 (41.54%), Postives = 41/65 (63.08%), Query Frame = 2 
Query:   68 VMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            V++ T +   ++C  P K+GPC+G FPR+++N    KC  F FGGCK N NN+ +++ C   C K
Sbjct:  225 VLVLTPEQTHHHCLVPKKAGPCRGSFPRWHYNAASEKCEEFTFGGCKENRNNYLSREECLNACDK 289

HSP 2 Score: 52.373 bits (124), Expect = 6.043e-9
Identity = 22/51 (43.14%), Postives = 30/51 (58.82%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            C  P  +GPC+  F ++Y+N Y  KC RF +GGC+ N N F T+  C   C
Sbjct:  371 CTQPPVTGPCRASFTKWYYNPYDRKCNRFNYGGCQGNDNQFETQDHCMKVC 421
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:103032340])

HSP 1 Score: 60.8474 bits (146), Expect = 6.066e-12
Identity = 27/65 (41.54%), Postives = 41/65 (63.08%), Query Frame = 2 
Query:   68 VMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            V++ T +   ++C  P K+GPC+G FPR+++N    KC  F FGGCK N NN+ +++ C   C K
Sbjct:  225 VLVLTPEQTHHHCLVPKKAGPCRGSFPRWHYNAASEKCEEFTFGGCKENRNNYLSREECLNACDK 289

HSP 2 Score: 51.9878 bits (123), Expect = 9.159e-9
Identity = 22/51 (43.14%), Postives = 30/51 (58.82%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            C  P  +GPC+  F ++Y+N Y  KC RF +GGC+ N N F T+  C   C
Sbjct:  358 CTQPPVTGPCRASFTKWYYNPYDRKCNRFNYGGCQGNDNQFETQDHCMKVC 408
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: lrp11 (LDL receptor related protein 11 [Source:NCBI gene;Acc:103032685])

HSP 1 Score: 60.077 bits (144), Expect = 1.064e-11
Identity = 24/54 (44.44%), Postives = 34/54 (62.96%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKK 265
            C +P   GPC+G FPR+Y++   G+C  F++GGCK N NNF  +  C   C +K
Sbjct:  417 CMEPPVVGPCRGTFPRWYYDAAAGECKHFLYGGCKGNRNNFLQQTDCVNECIQK 470
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: spint1b (kunitz-type protease inhibitor 1-like [Source:NCBI gene;Acc:103024868])

HSP 1 Score: 60.077 bits (144), Expect = 1.251e-11
Identity = 26/63 (41.27%), Postives = 36/63 (57.14%), Query Frame = 2 
Query:   68 VMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            V++ T +    +C  P K GPC+G FPR+++N    KC  F FGGC  NGNN+ +   C   C
Sbjct:  223 VLVLTAEQAEQHCLTPKKVGPCRGAFPRWHYNAASNKCEEFTFGGCLENGNNYLSSDDCVDAC 285

HSP 2 Score: 52.7582 bits (125), Expect = 4.432e-9
Identity = 22/59 (37.29%), Postives = 31/59 (52.54%), Query Frame = 2 
Query:   83 IKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCR 259
            +   +  C DP  +GPC+  F R+Y++     C RF +GGC  N NNF  +  C   CR
Sbjct:  327 VNEGKARCVDPPVTGPCRASFTRWYYDPLQQDCHRFTYGGCTGNKNNFEGQDKCMDACR 385
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:103022515])

HSP 1 Score: 60.077 bits (144), Expect = 1.415e-11
Identity = 23/61 (37.70%), Postives = 38/61 (62.30%), Query Frame = 2 
Query:   80 TIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            T++  +  C    ++GPC+   PR++F+    KCVRFI+GGC  N NNF +++ C   C++
Sbjct:  307 TLQEVKAVCSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMAVCKR 367
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 60.4622 bits (145), Expect = 7.889e-13
Identity = 26/59 (44.07%), Postives = 34/59 (57.63%), Query Frame = 2 
Query:   80 TIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            T++ QR  C  P K G C+  FPR++FN    +C  FI+GGC  N NNF T + C   C
Sbjct:   19 TLEDQRVTCTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECRAAC 77

HSP 2 Score: 44.669 bits (104), Expect = 5.406e-7
Identity = 17/54 (31.48%), Postives = 31/54 (57.41%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKK 265
            C+   ++G C+ +  R+++N  +G C+ F++GGC  N N F   + C   C +K
Sbjct:   87 CRPDAETGRCRAHITRYFYNMTLGMCMPFVYGGCGGNNNRFEDMESCVFSCIEK 140
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 58.5362 bits (140), Expect = 7.925e-12
Identity = 25/61 (40.98%), Postives = 40/61 (65.57%), Query Frame = 2 
Query:   74 MATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            MA + +    C  P++ GPCK +  R+ +N  I +C  FI+GGC+ N NNF++++LCE +C
Sbjct:  364 MACMANINNTCSLPVQQGPCKQWALRWAYNPLIKRCQSFIYGGCEGNENNFQSQELCEDNC 424
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.6766 bits (117), Expect = 8.390e-9
Identity = 21/52 (40.38%), Postives = 29/52 (55.77%), Query Frame = 2 
Query:  101 NCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +C   +  GPC+ Y PR ++N Y  +C  F +GGC  NGNNF +   C   C
Sbjct:    2 SCMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53

HSP 2 Score: 45.0542 bits (105), Expect = 3.524e-7
Identity = 20/51 (39.22%), Postives = 28/51 (54.90%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            C+  M+ G C+    R++FN   G C  F +GGC+ N NNF+    C  HC
Sbjct:   75 CRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 125
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 9.602e-9
Identity = 21/52 (40.38%), Postives = 29/52 (55.77%), Query Frame = 2 
Query:  101 NCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            +C   +  GPC+ Y PR ++N Y  +C  F +GGC  NGNNF +   C   C
Sbjct:    2 SCMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53

HSP 2 Score: 44.669 bits (104), Expect = 4.826e-7
Identity = 20/51 (39.22%), Postives = 28/51 (54.90%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            C+  M+ G C+    R++FN   G C  F +GGC+ N NNF+    C  HC
Sbjct:   74 CRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 124
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008261.1 (pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene:ENSPMAG00000007479.1 transcript:ENSPMAT00000008261.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 1.379e-8
Identity = 21/60 (35.00%), Postives = 32/60 (53.33%), Query Frame = 2 
Query:   77 ATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            A +      C  P   GPCK +  R+ +N    +C  F++GGC+ NGN+F ++  CE  C
Sbjct:   12 ACVNETARVCSLPPVQGPCKAWETRWSYNPLGKRCATFLYGGCEGNGNSFASRSACERAC 71
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO38284 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB9])

HSP 1 Score: 66.6254 bits (161), Expect = 2.427e-16
Identity = 33/63 (52.38%), Postives = 39/63 (61.90%), Query Frame = 2 
Query:   74 MATIKSQRYN--CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            M    S+R    CK P  +GPC     RFY+N  IGKC  F+FGGC PNGNNF+T+K CE  C
Sbjct:    1 MDVFISERIKSVCKLPKFAGPCMASIERFYYNAKIGKCQSFVFGGCLPNGNNFKTRKACEAKC 63
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 56.6102 bits (135), Expect = 1.918e-12
Identity = 23/52 (44.23%), Postives = 33/52 (63.46%), Query Frame = 2 
Query:  101 NCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
             C  P  +GPC+ YF R+++N    KC +F++GGC+ N NNF +K  CE  C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 56.6102 bits (135), Expect = 1.918e-12
Identity = 23/52 (44.23%), Postives = 33/52 (63.46%), Query Frame = 2 
Query:  101 NCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
             C  P  +GPC+ YF R+++N    KC +F++GGC+ N NNF +K  CE  C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO31759 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX08])

HSP 1 Score: 54.299 bits (129), Expect = 1.301e-11
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            CK P  +GPC+   P++Y+N    +C+RFI+GGC  N NNF TK  C+  C
Sbjct:    2 CKMPAAAGPCRAAMPQWYYNFKRHRCIRFIYGGCGGNPNNFDTKNECKSAC 52
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO29819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2J6])

HSP 1 Score: 52.7582 bits (125), Expect = 8.493e-11
Identity = 24/51 (47.06%), Postives = 29/51 (56.86%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            C  P K G C+   PR +FN   GKC  FI+GGC  N NNF T + C+  C
Sbjct:   14 CTLPKKVGDCRAAMPRVFFNSQSGKCEDFIYGGCGGNANNFHTMEECKKAC 64
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:101173105])

HSP 1 Score: 65.4698 bits (158), Expect = 1.421e-13
Identity = 30/81 (37.04%), Postives = 46/81 (56.79%), Query Frame = 2 
Query:   14 F*LLIEMKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            F L +      T   V  V++ T +   ++C  P K GPC+G FPR+++N    KC +FIFGGC+ N NN+  ++ C+  C
Sbjct:  209 FKLTVTDSIGQTDSTVLTVLVLTPQESEHHCMAPKKVGPCRGSFPRWHYNAASQKCEQFIFGGCRENRNNYLLEEECKFAC 289
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:101173105])

HSP 1 Score: 65.4698 bits (158), Expect = 1.425e-13
Identity = 30/81 (37.04%), Postives = 46/81 (56.79%), Query Frame = 2 
Query:   14 F*LLIEMKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            F L +      T   V  V++ T +   ++C  P K GPC+G FPR+++N    KC +FIFGGC+ N NN+  ++ C+  C
Sbjct:  209 FKLTVTDSIGQTDSTVLTVLVLTPQESEHHCMAPKKVGPCRGSFPRWHYNAASQKCEQFIFGGCRENRNNYLLEEECKFAC 289
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: spint1a (kunitz-type protease inhibitor 1 [Source:NCBI gene;Acc:101175207])

HSP 1 Score: 62.7734 bits (151), Expect = 1.295e-12
Identity = 28/66 (42.42%), Postives = 40/66 (60.61%), Query Frame = 2 
Query:   59 VFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHC 256
            V  V++ T +    +C  P K GPC+G FPR+Y+N    KC  F FGGC+ N NN+ T++ C + C
Sbjct:  219 VVTVLVLTPEQSMDHCLVPKKVGPCRGSFPRWYYNAASEKCETFFFGGCRQNKNNYLTEEECAMAC 284
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:101168561])

HSP 1 Score: 58.151 bits (139), Expect = 5.029e-11
Identity = 22/53 (41.51%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C    ++GPC+   PR++F+    KCVRFI+GGC  N NNF +++ C   C++
Sbjct:  314 CTLEAETGPCRASMPRWHFDLSQRKCVRFIYGGCAGNRNNFDSEEYCMAVCKR 366
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:101168561])

HSP 1 Score: 58.151 bits (139), Expect = 5.035e-11
Identity = 22/53 (41.51%), Postives = 34/53 (64.15%), Query Frame = 2 
Query:  104 CKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            C    ++GPC+   PR++F+    KCVRFI+GGC  N NNF +++ C   C++
Sbjct:  314 CTLEAETGPCRASMPRWHFDLSQRKCVRFIYGGCAGNRNNFDSEEYCMAVCKR 366
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000038004.1 (SMESG000038004.1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.251e-21
Identity = 37/77 (48.05%), Postives = 50/77 (64.94%), Query Frame = 2 
Query:   32 MKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            MK LI F  +  +M   I+   +NC DP  SGPC GY  R+Y+N+   KC +FI+GGCKPNGNNF+T + C+  C +
Sbjct:    1 MKVLIKFIPLSCLMFFAIQCLEHNCDDPKTSGPCYGYILRYYYNNNTTKCEKFIYGGCKPNGNNFKTLEACQDFCEE 77
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000037997.1 (SMESG000037997.1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.690e-20
Identity = 34/83 (40.96%), Postives = 50/83 (60.24%), Query Frame = 2 
Query:   26 IEMKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKRHQ 274
            ++ K +I    V   ++ TI+S   NC+DP+  G C+ YFPR+++N    KC RFI+GGC  NGNNF+T K C+  C +    
Sbjct:    1 MKTKLIIWLALVLSFIIFTIESLEPNCEDPLVKGLCEAYFPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLCEETNSD 83
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000038002.1 (SMESG000038002.1)

HSP 1 Score: 77.411 bits (189), Expect = 4.163e-20
Identity = 34/83 (40.96%), Postives = 50/83 (60.24%), Query Frame = 2 
Query:   26 IEMKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKRHQ 274
            ++ K +I    V   ++ TI+S   NC+DP+  G C+ YFPR+++N    KC RFI+GGC  NGNNF+T K C+  C +    
Sbjct:    1 MKTKLIICLALVLSFIIFTIESLEPNCEDPLVKGLCEAYFPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLCEETNSD 83
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000037996.1 (SMESG000037996.1)

HSP 1 Score: 78.5666 bits (192), Expect = 8.213e-20
Identity = 35/83 (42.17%), Postives = 51/83 (61.45%), Query Frame = 2 
Query:   26 IEMKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKRHQ 274
            ++ K +I    V   ++ TI+S   NC+DP+  G CK Y PR+++N    KC RFI+GGC  NGNNF+T K C+  C ++  Q
Sbjct:    1 MKTKLIICLPLVLSFIIFTIESLEQNCEDPLAKGLCKAYIPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLCEERNKQ 83

HSP 2 Score: 64.3142 bits (155), Expect = 2.330e-14
Identity = 27/63 (42.86%), Postives = 38/63 (60.32%), Query Frame = 2 
Query:   86 KSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRKKRHQ 274
            +  + NC+DP+  G CK Y PR+++N    KC RFI+GGC  NGNNF+  K C+  C +    
Sbjct:   79 ERNKQNCEDPLAKGFCKAYIPRYFYNDTSEKCERFIYGGCGANGNNFKILKDCQDFCEETNSD 141
[Close]
BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000037994.1 (SMESG000037994.1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.422e-19
Identity = 34/77 (44.16%), Postives = 47/77 (61.04%), Query Frame = 2 
Query:   32 MKFLITFCFVFLVMMATIKSQRYNCKDPMKSGPCKGYFPRFYFNHYIGKCVRFIFGGCKPNGNNFRTKKLCEIHCRK 262
            M  ++ F  +F V+ +  +S+   C +P K+G C  YFPRFYFN   G C  FI+GGC+ NGNNF T + CE  C +
Sbjct:    1 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 77
The following BLAST results are available for this feature:
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
WFIKKN21.332e-1241.94WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
APLP21.543e-1246.15amyloid beta precursor like protein 2 [Source:HGNC... [more]
APLP21.545e-1246.15amyloid beta precursor like protein 2 [Source:HGNC... [more]
APLP21.545e-1246.15amyloid beta precursor like protein 2 [Source:HGNC... [more]
WFIKKN22.251e-1241.94WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mlt-113.952e-1147.17pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
mlt-114.030e-1147.17pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
mlt-114.571e-1147.17pep chromosome:WBcel235:V:20667209:20678513:1 gene... [more]
mlt-116.225e-1147.17pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
mlt-117.623e-1147.17pep chromosome:WBcel235:V:20667209:20678513:1 gene... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG173803.092e-1035.71gene:FBgn0039077 transcript:FBtr0299867[more]
Ppn4.408e-1043.40gene:FBgn0003137 transcript:FBtr0300337[more]
Ppn5.990e-1043.40gene:FBgn0003137 transcript:FBtr0301838[more]
Ppn6.230e-1043.40gene:FBgn0003137 transcript:FBtr0301839[more]
Ppn7.142e-1043.40gene:FBgn0003137 transcript:FBtr0301837[more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
aplp28.361e-1237.70amyloid beta (A4) precursor-like protein 2 [Source... [more]
aplp29.592e-1237.70amyloid beta (A4) precursor-like protein 2 [Source... [more]
spint1a1.362e-1140.00serine peptidase inhibitor, Kunitz type 1 a [Sourc... [more]
spint1a1.367e-1140.00serine peptidase inhibitor, Kunitz type 1 a [Sourc... [more]
spint1a1.367e-1140.00serine peptidase inhibitor, Kunitz type 1 a [Sourc... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
APLP22.370e-1144.23amyloid beta (A4) precursor-like protein 2 [Source... [more]
APLP22.371e-1144.23amyloid beta (A4) precursor-like protein 2 [Source... [more]
APLP22.372e-1144.23amyloid beta (A4) precursor-like protein 2 [Source... [more]
APLP22.373e-1144.23amyloid beta (A4) precursor-like protein 2 [Source... [more]
APLP22.373e-1144.23amyloid beta (A4) precursor-like protein 2 [Source... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Aplp21.059e-1246.15amyloid beta (A4) precursor-like protein 2 [Source... [more]
Aplp21.891e-1246.15amyloid beta (A4) precursor-like protein 2 [Source... [more]
Wfdc6a4.589e-1241.82WAP four-disulfide core domain 6A [Source:MGI Symb... [more]
Wfdc6a4.589e-1241.82WAP four-disulfide core domain 6A [Source:MGI Symb... [more]
Wfikkn26.089e-1241.94WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P0DMJ5|VKT_HETCR2.981e-1542.31PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=17577... [more]
sp|C0HJF3|VKT3_HETCR1.669e-1456.60TauPI-stichotoxin-Hcr2d OS=Heteractis crispa OX=17... [more]
sp|B2G331|VKT1_HETCR2.605e-1441.03TauPI-stichotoxin-Hcr2b OS=Heteractis crispa OX=17... [more]
sp|C0HK74|VKT3_HETMG3.907e-1454.72Kunitz-type serine protease inhibitor HMGS2 OS=Het... [more]
sp|P81902|CSTI_BOMMO5.352e-1451.92Trypsin inhibitor OS=Bombyx mori OX=7091 PE=1 SV=1[more]
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BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3M6TIL32.331e-1443.04BPTI/Kunitz inhibitor domain-containing protein OS... [more]
A0A4Y2DL814.696e-1443.68Carboxypeptidase inhibitor SmCI OS=Araneus ventric... [more]
I1ZII06.738e-1445.21Serine protease inhibitor-1 (Fragment) OS=Schmidte... [more]
Q8T7L97.381e-1440.00Kazal-type serine proteinase inhibitor 1 OS=Bombyx... [more]
A0A151HYY22.147e-1337.04Taicatoxin, serine protease inhibitor component OS... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spint1a3.964e-1241.54serine peptidase inhibitor, Kunitz type 1 [Source:... [more]
spint1a6.066e-1241.54serine peptidase inhibitor, Kunitz type 1 [Source:... [more]
lrp111.064e-1144.44LDL receptor related protein 11 [Source:NCBI gene;... [more]
spint1b1.251e-1141.27kunitz-type protease inhibitor 1-like [Source:NCBI... [more]
aplp21.415e-1137.70amyloid beta precursor like protein 2 [Source:NCBI... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tfpi27.889e-1344.07tissue factor pathway inhibitor 2 [Source:ZFIN;Acc... [more]
WFIKKN17.925e-1240.98WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
ENSPMAT00000005038.18.390e-940.38pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g... [more]
ENSPMAT00000005043.19.602e-940.38pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g... [more]
ENSPMAT00000008261.11.379e-835.00pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO382842.427e-1652.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO416961.918e-1244.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO262631.918e-1244.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO317591.301e-1147.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO298198.493e-1147.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spint1a1.421e-1337.04serine peptidase inhibitor, Kunitz type 1 [Source:... [more]
spint1a1.425e-1337.04serine peptidase inhibitor, Kunitz type 1 [Source:... [more]
spint1a1.295e-1242.42kunitz-type protease inhibitor 1 [Source:NCBI gene... [more]
aplp25.029e-1141.51amyloid beta precursor like protein 2 [Source:NCBI... [more]
aplp25.035e-1141.51amyloid beta precursor like protein 2 [Source:NCBI... [more]
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BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038004.15.251e-2148.05SMESG000038004.1[more]
SMESG000037997.12.690e-2040.96SMESG000037997.1[more]
SMESG000038002.14.163e-2040.96SMESG000038002.1[more]
SMESG000037996.18.213e-2042.17SMESG000037996.1[more]
SMESG000037994.11.422e-1944.16SMESG000037994.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021397 ID=SMED30021397|Name=Kunitz-type serine protease inhibitor|organism=Schmidtea mediterranea sexual|type=transcript|length=310bp
CGCTCTTCCGATCTTTTGACTCTTAATCGAGATGAAATTTTTAATAACCT
TTTGTTTTGTTTTTTTGGTGATGATGGCTACGATTAAGAGTCAAAGATAT
AACTGTAAAGACCCAATGAAGTCTGGTCCGTGTAAGGGATATTTTCCTAG
ATTTTATTTCAATCATTATATTGGGAAATGCGTAAGATTTATCTTTGGAG
GTTGCAAACCAAATGGGAATAATTTCCGTACAAAAAAACTATGTGAAATA
CATTGTAGAAAAAAAAGACATCAGTTTTGAATGAAAAATAAATATTTAAT
TATTTAAAAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR002223Kunitz_BPTI
IPR020901Prtase_inh_Kunz-CS
Vocabulary: molecular function
TermDefinition
GO:0004867serine-type endopeptidase inhibitor activity
GO:0008233peptidase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0010951negative regulation of endopeptidase activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane