SMED30021389

Overview


Name	SMED30021389
Smed ID	SMED30021389
Length (bp)	678

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30021389 (SMED30021389) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021389 (SMED30021389) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021389 (SMED30021389) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021389 (SMED30021389) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30021389

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 17

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
epidermis	SMED30021389	SMESG000011789.1	dd_Smed_v4_3826_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30021389	SMESG000011789.1	dd_Smed_v4_3826_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30021389	SMESG000011789.1	dd_Smed_v4_3826_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30021389	SMESG000080676.1 SMESG000011789.1	Contig33124	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30021389	SMESG000080676.1 SMESG000011789.1	Contig33124	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30021389	SMESG000011789.1	Contig42143	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30021389	SMESG000011789.1	Contig42143	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
oocyte	SMED30021389	SMESG000080676.1 SMESG000011789.1	Contig33124	newmark_ests	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30021389	SMESG000080676.1 SMESG000011789.1	Contig33124	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30021389	SMESG000080676.1 SMESG000011789.1	Contig33124	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30021389	SMESG000080676.1 SMESG000011789.1	Contig33124	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
ovary	SMED30021389	SMESG000053363.1 SMESG000053360.1 SMESG000001725.1	Contig2831	GPL15192	PMID:28434803 Rouhana et al., 2017
testis	SMED30021389	SMESG000053363.1 SMESG000053360.1 SMESG000001725.1	Contig2831	GPL15192	PMID:28434803 Rouhana et al., 2017
ovary	SMED30021389	SMESG000011789.1	PL04007A1C09	ncbi_smed_ests	PMID:28434803 Rouhana et al., 2017
testis	SMED30021389	SMESG000011789.1	PL04007A1C09	ncbi_smed_ests	PMID:28434803 Rouhana et al., 2017
ovary	SMED30021389	SMESG000011789.1	Contig2831	GPL15192	PMID:28434803 Rouhana et al., 2017
testis	SMED30021389	SMESG000011789.1	Contig2831	GPL15192	PMID:28434803 Rouhana et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30021389 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000280:123820..124677 -	3250

Homology

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BLAST of SMED30021389 vs. Ensembl Human
Match: SUGT1 (SGT1 homolog, MIS12 kinetochore complex assembly cochaperone [Source:HGNC Symbol;Acc:HGNC:16987])

HSP 1 Score: 131.339 bits (329), Expect = 4.121e-36
Identity = 80/195 (41.03%), Postives = 115/195 (58.97%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSV-ELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEK----------VVEIK------PKINKNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K K++WYQT S V I L+IKNV  + + + F+E  +  L      E ++   EL   I PE S++K+ STK+EIKL K + + W  LE           V ++K          +NWD+++  +  E ++     +  LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNWS+V +++VE  PPD ME+KKY
Sbjct:  139 KIKYDWYQTESQVVITLMIKNVQKNDVNVEFSEKELSALVKLPSGEDYNLKLELLHPIIPEQSTFKVLSTKIEIKLKKPEAVRWEKLEGQGDVPTPKQFVADVKNLYPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 333

[Close]

BLAST of SMED30021389 vs. Ensembl Human
Match: SUGT1 (SGT1 homolog, MIS12 kinetochore complex assembly cochaperone [Source:HGNC Symbol;Acc:HGNC:16987])

HSP 1 Score: 130.954 bits (328), Expect = 8.174e-36
Identity = 80/195 (41.03%), Postives = 115/195 (58.97%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSV-ELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEK----------VVEIK------PKINKNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K K++WYQT S V I L+IKNV  + + + F+E  +  L      E ++   EL   I PE S++K+ STK+EIKL K + + W  LE           V ++K          +NWD+++  +  E ++     +  LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNWS+V +++VE  PPD ME+KKY
Sbjct:  171 KIKYDWYQTESQVVITLMIKNVQKNDVNVEFSEKELSALVKLPSGEDYNLKLELLHPIIPEQSTFKVLSTKIEIKLKKPEAVRWEKLEGQGDVPTPKQFVADVKNLYPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 365

[Close]

BLAST of SMED30021389 vs. Ensembl Human
Match: CHORDC1 (cysteine and histidine rich domain containing 1 [Source:HGNC Symbol;Acc:HGNC:14525])

HSP 1 Score: 51.9878 bits (123), Expect = 4.793e-8
Identity = 29/87 (33.33%), Postives = 50/87 (57.47%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            +H+W+QT   VTI +  KN    L ++  N T  +V + ++ E E F    +L+G I+ + S   + +TK+EI + K +P+ W +LE
Sbjct:   43 RHDWHQTGGEVTISVYAKNSLPELSRVEANSTLLNVHIVFEGEKE-FDQNVKLWGVIDVKRSYVTMTATKIEITMRKAEPMQWASLE 128

[Close]

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            +H+W+QT   VTI +  KN    L ++  N T  +V + ++ E E F    +L+G I+ + S   + +TK+EI + K +P+ W +LE
Sbjct:   43 RHDWHQTGGEVTISVYAKNSLPELSRVEANSTLLNVHIVFEGEKE-FDQNVKLWGVIDVKRSYVTMTATKIEITMRKAEPMQWASLE 128

[Close]

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            +H+W+QT   VTI +  KN    L ++  N T  +V + ++ E E F    +L+G I+ + S   + +TK+EI + K +P+ W +LE
Sbjct:   43 RHDWHQTGGEVTISVYAKNSLPELSRVEANSTLLNVHIVFEGEKE-FDQNVKLWGVIDVKRSYVTMTATKIEITMRKAEPMQWASLE 128

[Close]

BLAST of SMED30021389 vs. Ensembl Celegans
Match: D1054.3 (pep chromosome:WBcel235:V:10771451:10772832:1 gene:WBGene00008371.1 transcript:D1054.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:D1054.3)

HSP 1 Score: 106.686 bits (265), Expect = 1.622e-28
Identity = 66/201 (32.84%), Postives = 112/201 (55.72%), Query Frame = -1

Query:  100 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDS-----ESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPK-KDPINWINLEK--------VVEIKP--------KINKNWDQIMNGLSNELEDD----NDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYD 624
            K +H+W+Q+ + VT+ +L + V         ++ SV LS ++     + ++     +L G++  ++ + K  + KVE++LPK      W +L K        V  + P         + KNWD I      E ED+    +  +N +F+K+Y D +D+ R+AM+KS++ES+GTVLSTNWSE+ +K+ E +PP  MEYK+Y+
Sbjct:    4 KPRHDWFQSETDVTLTILKRGVP-------LDDCSVSLSDNNTLTVKQCDEILFYGQLSGQVKKDDLTVKCTAAKVEVRLPKFARNERWASLLKDGQGVAASVQSVSPNPESAPTTTVKKNWDAIEKQAVKEEEDESLEGDAAVNKMFRKMYNDASDDVRRAMMKSYSESNGTVLSTNWSEIGQKKTECQPPACMEYKEYE 197

[Close]

BLAST of SMED30021389 vs. Ensembl Celegans
Match: D1054.3 (pep chromosome:WBcel235:V:10771551:10772832:1 gene:WBGene00008371.1 transcript:D1054.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:D1054.3)

HSP 1 Score: 77.7962 bits (190), Expect = 5.472e-19
Identity = 31/55 (56.36%), Postives = 47/55 (85.45%), Query Frame = -1

Query:  100 LFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYD 264
            +F+K+Y D +D+ R+AM+KS++ES+GTVLSTNWSE+ +K+ E +PP  MEYK+Y+
Sbjct:    1 MFRKMYNDASDDVRRAMMKSYSESNGTVLSTNWSEIGQKKTECQPPACMEYKEYE 55

[Close]

BLAST of SMED30021389 vs. Ensembl Fly
Match: Sgt1 (gene:FBgn0265101 transcript:FBtr0081862)

HSP 1 Score: 120.553 bits (301), Expect = 4.977e-34
Identity = 70/179 (39.11%), Postives = 107/179 (59.78%), Query Frame = -1

Query:  100 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVV---EIKPKINKNWDQIMN---GLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYD 618
            +H+WYQ+ + V I +L+KN       +   +  V ++ D     +    +L   I  E SSYK +STKVEI L K+  I W NLE+ +    +KPK  KNWDQ+++    +  +       L +LF+KIY   + E +KAM KSF+ES GTVLSTNW+EV +++V  KPP+G E+++++
Sbjct:    4 RHDWYQSETKVVITVLLKNAVDKNYAVEITQKRVHMTADG----YELDLKLLHPIVVERSSYKAFSTKVEITLAKETGIRWENLEEAIVAAPVKPKA-KNWDQLVSEEEKIDEKEAKGEAALTNLFKKIYSSSSPEVQKAMNKSFSESGGTVLSTNWNEVGKERVTVKPPNGTEFREWE 177

[Close]

BLAST of SMED30021389 vs. Ensembl Fly
Match: CHORD (gene:FBgn0029503 transcript:FBtr0084554)

HSP 1 Score: 53.1434 bits (126), Expect = 4.614e-8
Identity = 30/89 (33.71%), Postives = 50/89 (56.18%), Query Frame = -1

Query:  367 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSY---DSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINL 624
            + +++W+QT + V + +  K  D S   I  N   + ++    + ++ +F    EL G +N  N+S  +Y TKVEIKLPK +P +W NL
Sbjct:  212 QCRYDWHQTATNVVMAIYAKKYDYSQSVIELNPIRLHVNLVFPEQDNARFDLDLELRGIVNVSNASAHMYGTKVEIKLPKLEPGSWSNL 300

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BLAST of SMED30021389 vs. Ensembl Zebrafish
Match: sugt1 (SGT1 homolog, MIS12 kinetochore complex assembly cochaperone [Source:ZFIN;Acc:ZDB-GENE-041114-56])

HSP 1 Score: 123.635 bits (309), Expect = 1.398e-33
Identity = 76/191 (39.79%), Postives = 107/191 (56.02%), Query Frame = -1

Query:  103 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSES-EKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINL---EKVVEIK----------PKINKNWDQIMNGLSNE-----LEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 618
            KH+WYQT S V + +++KN     + + F E  +       S E +     L   I P+ S+YKI STK+EIK+ K + I W  L   E +  +K              +NWD+++  +  E     LE D   LN LFQ+IY DG+D+ R+AM KSF ES GTVLSTNW +V +++VE  PPD +E+KKY
Sbjct:  133 KHDWYQTESQVAVTIMVKNAKKEGVIVSFGERELTAVVKLPSGEDYCLKVHLLHPIVPQQSTYKILSTKIEIKMKKTEAIQWEKLQGEETLSNVKHFTASQYPSSSHYTRNWDKLVGEIKEEEKKENLEGDA-ALNKLFQQIYSDGSDDVRRAMNKSFMESGGTVLSTNWIDVGKRKVEMNPPDDVEWKKY 322

[Close]

BLAST of SMED30021389 vs. Ensembl Zebrafish
Match: sugt1 (SGT1 homolog, MIS12 kinetochore complex assembly cochaperone [Source:ZFIN;Acc:ZDB-GENE-041114-56])

HSP 1 Score: 123.635 bits (309), Expect = 2.060e-33
Identity = 76/191 (39.79%), Postives = 107/191 (56.02%), Query Frame = -1

Query:  103 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSES-EKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINL---EKVVEIK----------PKINKNWDQIMNGLSNE-----LEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 618
            KH+WYQT S V + +++KN     + + F E  +       S E +     L   I P+ S+YKI STK+EIK+ K + I W  L   E +  +K              +NWD+++  +  E     LE D   LN LFQ+IY DG+D+ R+AM KSF ES GTVLSTNW +V +++VE  PPD +E+KKY
Sbjct:  141 KHDWYQTESQVAVTIMVKNAKKEGVIVSFGERELTAVVKLPSGEDYCLKVHLLHPIVPQQSTYKILSTKIEIKMKKTEAIQWEKLQGEETLSNVKHFTASQYPSSSHYTRNWDKLVGEIKEEEKKENLEGDA-ALNKLFQQIYSDGSDDVRRAMNKSFMESGGTVLSTNWIDVGKRKVEMNPPDDVEWKKY 330

[Close]

BLAST of SMED30021389 vs. Ensembl Xenopus
Match: pnpla3 (patatin like phospholipase domain containing 3 [Source:Xenbase;Acc:XB-GENE-5855123])

HSP 1 Score: 135.576 bits (340), Expect = 5.505e-38
Identity = 83/195 (42.56%), Postives = 114/195 (58.46%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKPK---------------INKNWD----QIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K++H+WYQT S + I ++IKNV  + + I F+E   +V +S  S  E +S    L   I P+ S +K+ STKVEIKL K + + W  LE   + + K                 KNWD    QI     NE  + +  LN LFQ+IY DGNDE ++AM KSF ES GTVLSTNW++V +K+V+  PPD ME+K+Y
Sbjct:  123 KYRHDWYQTESHIIITVMIKNVQKNNVHIRFSERELTVNMSLPS-GENYSLNLHLLHAIVPDQSIFKVLSTKVEIKLKKTEAMRWETLEGKADSQVKHFTQESMHKYPSSSHYTKNWDKLVGQIKEEEKNEKLEGDAALNQLFQQIYSDGNDEVKRAMNKSFMESGGTVLSTNWTDVGKKKVDVNPPDDMEWKQY 316

[Close]

BLAST of SMED30021389 vs. Ensembl Xenopus
Match: pnpla3 (patatin like phospholipase domain containing 3 [Source:Xenbase;Acc:XB-GENE-5855123])

HSP 1 Score: 135.576 bits (340), Expect = 7.858e-38
Identity = 83/195 (42.56%), Postives = 114/195 (58.46%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKPK---------------INKNWD----QIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K++H+WYQT S + I ++IKNV  + + I F+E   +V +S  S  E +S    L   I P+ S +K+ STKVEIKL K + + W  LE   + + K                 KNWD    QI     NE  + +  LN LFQ+IY DGNDE ++AM KSF ES GTVLSTNW++V +K+V+  PPD ME+K+Y
Sbjct:  137 KYRHDWYQTESHIIITVMIKNVQKNNVHIRFSERELTVNMSLPS-GENYSLNLHLLHAIVPDQSIFKVLSTKVEIKLKKTEAMRWETLEGKADSQVKHFTQESMHKYPSSSHYTKNWDKLVGQIKEEEKNEKLEGDAALNQLFQQIYSDGNDEVKRAMNKSFMESGGTVLSTNWTDVGKKKVDVNPPDDMEWKQY 330

[Close]

BLAST of SMED30021389 vs. Ensembl Xenopus
Match: itgb1bp2 (lymphoid-restricted membrane protein-like [Source:Xenbase;Acc:XB-GENE-6462797])

HSP 1 Score: 58.5362 bits (140), Expect = 1.350e-9
Identity = 36/104 (34.62%), Postives = 58/104 (55.77%), Query Frame = -1

Query:  334 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETS--VELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEK-------VVEIKPKIN 618
            +++W+QT SVV I +  K     L  ++ N+T+  +++S+  +SE F    EL+G I+   S+  +  TKVEI + K DP+ W  LE         VE +P+IN
Sbjct:  225 RYDWHQTSSVVVITIYAKTAIPELSHVLANQTALDIQISFQGQSE-FRKQVELWGVIDIGKSTVSLLPTKVEITMKKADPVTWGRLELSRHSPQITVEEEPQIN 327

[Close]

BLAST of SMED30021389 vs. Ensembl Xenopus
Match: itgb1bp2 (lymphoid-restricted membrane protein-like [Source:Xenbase;Acc:XB-GENE-6462797])

HSP 1 Score: 58.151 bits (139), Expect = 1.704e-9
Identity = 36/104 (34.62%), Postives = 58/104 (55.77%), Query Frame = -1

Query:  334 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETS--VELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEK-------VVEIKPKIN 618
            +++W+QT SVV I +  K     L  ++ N+T+  +++S+  +SE F    EL+G I+   S+  +  TKVEI + K DP+ W  LE         VE +P+IN
Sbjct:  246 RYDWHQTSSVVVITIYAKTAIPELSHVLANQTALDIQISFQGQSE-FRKQVELWGVIDIGKSTVSLLPTKVEITMKKADPVTWGRLELSRHSPQITVEEEPQIN 348

[Close]

BLAST of SMED30021389 vs. Ensembl Xenopus
Match: itgb1bp2 (lymphoid-restricted membrane protein-like [Source:Xenbase;Acc:XB-GENE-6462797])

HSP 1 Score: 58.151 bits (139), Expect = 1.984e-9
Identity = 36/104 (34.62%), Postives = 58/104 (55.77%), Query Frame = -1

Query:  334 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETS--VELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEK-------VVEIKPKIN 618
            +++W+QT SVV I +  K     L  ++ N+T+  +++S+  +SE F    EL+G I+   S+  +  TKVEI + K DP+ W  LE         VE +P+IN
Sbjct:  233 RYDWHQTSSVVVITIYAKTAIPELSHVLANQTALDIQISFQGQSE-FRKQVELWGVIDIGKSTVSLLPTKVEITMKKADPVTWGRLELSRHSPQITVEEEPQIN 335

[Close]

BLAST of SMED30021389 vs. Ensembl Mouse
Match: Sugt1 (SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:1915205])

HSP 1 Score: 128.257 bits (321), Expect = 4.384e-35
Identity = 78/195 (40.00%), Postives = 115/195 (58.97%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSV-ELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE-KVVEIKPK---------------INKNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K K++WYQT S V I L+IK+V  + +++ F+E  +  L      E +S    L   I PE S++K+ STK+EIK+ K + + W  LE +  E  PK                 +NWD+++  +  E ++     +  LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNWS+V +++VE  PPD ME+K+Y
Sbjct:  142 KIKYDWYQTESHVIITLMIKSVQKNDVRVGFSERELSALVKIPAGEDYSLKLRLLHPIIPEQSTFKVLSTKIEIKMKKPEAVRWEKLEGQGDEPTPKQFTADVKNMYPSSSHYTRNWDKLVGEIKEEEKNEKLEGDAALNKLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKQY 336

[Close]

BLAST of SMED30021389 vs. Ensembl Mouse
Match: Chordc1 (cysteine and histidine-rich domain (CHORD)-containing, zinc-binding protein 1 [Source:MGI Symbol;Acc:MGI:1914167])

HSP 1 Score: 50.0618 bits (118), Expect = 7.581e-7
Identity = 29/87 (33.33%), Postives = 50/87 (57.47%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            +H+W+QT   VTI +  KN    L ++  N T  +V + ++ E E F    +L+G I+ + S   + +TK+EI + K +P+ W +LE
Sbjct:  231 RHDWHQTGGEVTISVYAKNSLPELSQVEANSTLLNVHIVFEGEKE-FHQNVKLWGVIDVKRSYVTMTATKIEITMRKAEPMQWASLE 316

[Close]

BLAST of SMED30021389 vs. UniProt/SwissProt
Match: sp|Q9Y2Z0|SGT1_HUMAN (Protein SGT1 homolog OS=Homo sapiens OX=9606 GN=SUGT1 PE=1 SV=3)

HSP 1 Score: 130.954 bits (328), Expect = 3.925e-35
Identity = 80/195 (41.03%), Postives = 115/195 (58.97%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSV-ELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEK----------VVEIK------PKINKNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K K++WYQT S V I L+IKNV  + + + F+E  +  L      E ++   EL   I PE S++K+ STK+EIKL K + + W  LE           V ++K          +NWD+++  +  E ++     +  LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNWS+V +++VE  PPD ME+KKY
Sbjct:  171 KIKYDWYQTESQVVITLMIKNVQKNDVNVEFSEKELSALVKLPSGEDYNLKLELLHPIIPEQSTFKVLSTKIEIKLKKPEAVRWEKLEGQGDVPTPKQFVADVKNLYPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 365

[Close]

BLAST of SMED30021389 vs. UniProt/SwissProt
Match: sp|Q2KIK0|SGT1_BOVIN (Protein SGT1 homolog OS=Bos taurus OX=9913 GN=SUGT1 PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 7.525e-35
Identity = 79/195 (40.51%), Postives = 115/195 (58.97%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSV-ELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEI---KPKI-------------NKNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K K++WYQT S V I L+IKNV  + + + F+E  +  L      + +S    L   I PE S++K+ STK+EIK+ K + + W  LE   ++   KP I              +NWD+++  +  E ++     +  LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNWS+V +++VE  PPD ME+KKY
Sbjct:  144 KIKYDWYQTESQVIITLMIKNVQKNDVNVEFSEKELSALVKLPSGDDYSLKLRLLHPIIPEQSTFKVLSTKIEIKMKKPEAVRWEKLEGQGDVPNPKPFIADVKNLYPSSSHYTRNWDKLVGEIKEEEKNEKLEGDAALNKLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 338

[Close]

BLAST of SMED30021389 vs. UniProt/SwissProt
Match: sp|Q9CX34|SGT1_MOUSE (Protein SGT1 homolog OS=Mus musculus OX=10090 GN=Sugt1 PE=1 SV=3)

HSP 1 Score: 128.257 bits (321), Expect = 3.067e-34
Identity = 78/195 (40.00%), Postives = 115/195 (58.97%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSV-ELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE-KVVEIKPK---------------INKNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K K++WYQT S V I L+IK+V  + +++ F+E  +  L      E +S    L   I PE S++K+ STK+EIK+ K + + W  LE +  E  PK                 +NWD+++  +  E ++     +  LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNWS+V +++VE  PPD ME+K+Y
Sbjct:  142 KIKYDWYQTESHVIITLMIKSVQKNDVRVGFSERELSALVKIPAGEDYSLKLRLLHPIIPEQSTFKVLSTKIEIKMKKPEAVRWEKLEGQGDEPTPKQFTADVKNMYPSSSHYTRNWDKLVGEIKEEEKNEKLEGDAALNKLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKQY 336

[Close]

BLAST of SMED30021389 vs. UniProt/SwissProt
Match: sp|B0BN85|SGT1_RAT (Protein SGT1 homolog OS=Rattus norvegicus OX=10116 GN=Sugt1 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 3.165e-34
Identity = 78/195 (40.00%), Postives = 114/195 (58.46%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSES-EKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEI-KPK---------------INKNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            K K++WYQT S V I L+IKNV  + +++ F+E  +       S E  S    L   I PE S++K+ STK+EIK+ K + + W  LE   ++  PK                 +NWD+++  +  E ++     +  LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNWS+V +++VE  PPD ME+K+Y
Sbjct:  142 KIKYDWYQTESHVIITLMIKNVQKNDVRVDFSEKELSAVVKIPSGEDCSLKLRLLHPIIPEQSTFKVLSTKIEIKMKKPEAVRWEKLEGQGDVPAPKQFTADVKNMYPSSSHYTRNWDKLVGEIKEEEKNEKLEGDAALNKLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKQY 336

[Close]

BLAST of SMED30021389 vs. UniProt/SwissProt
Match: sp|Q9SUR9|SGT1A_ARATH (Protein SGT1 homolog A OS=Arabidopsis thaliana OX=3702 GN=SGT1A PE=1 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 9.675e-32
Identity = 72/200 (36.00%), Postives = 111/200 (55.50%), Query Frame = -1

Query:   97 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESE-KFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE--KVVEIKPKIN-----------------KNWDQIMNGLSNELEDD----NDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYDI 624
            K++HE+YQ P  V + +  K +    + I F E  + +  +   E  +     LFG+I P+   Y++ STK+EI L K D I W +LE  K   + PK N                 K+WD++   +  + +D+    +  LN  F++IY++ +++ R+AM KSF ES+GTVLSTNW EV  K +E+ PPDGME KK++I
Sbjct:  151 KYRHEYYQKPEEVVVTVFAKGIPKQNVNIDFGEQILSVVIEVPGEDAYYLQPRLFGKIIPDKCKYEVLSTKIEICLAKADIITWASLEHGKGPAVLPKPNVSSEVSQRPAYPSSKKVKDWDKLEAEVKKQEKDEKLEGDAALNKFFREIYQNADEDMRRAMSKSFVESNGTVLSTNWQEVGTKTIESTPPDGMELKKWEI 350

[Close]

BLAST of SMED30021389 vs. TrEMBL
Match: A0A1B6CNN1 (Uncharacterized protein OS=Clastoptera arizonana OX=38151 GN=g.2058 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 5.918e-45
Identity = 87/185 (47.03%), Postives = 124/185 (67.03%), Query Frame = -1

Query:  100 SMNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESE-KFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKP----KINKNWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYD 639
            S+N    KHEWYQT + +TI  LIKN+    + + +   S+++      E  ++H F  FG I PE  SYKI  +KVE+KL K D + W  LE+  ++      K++KNWD+++     E E + D +N+LFQKIY +G+DE +KAMIKSFTES+GTVLSTNW+E+ + +VE+KPPDGME+KK+D
Sbjct:    7 SVNNISVKHEWYQTETHLTIVFLIKNMRQDNVIVNYFTRSLQIIIKINVEDTYNHVFNFFGSICPEQCSYKILQSKVEVKLKKNDGLMWPMLEENHKVSSNATNKLSKNWDKVIKD-EVENEQEGDSVNNLFQKIYGEGDDECKKAMIKSFTESNGTVLSTNWNEISKNKVESKPPDGMEWKKWD 190

[Close]

BLAST of SMED30021389 vs. TrEMBL
Match: A0A4E0QY98 (Suppressor of G2 allele of SKP1 OS=Fasciola hepatica OX=6192 GN=D915_009002 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.096e-42
Identity = 89/198 (44.95%), Postives = 119/198 (60.10%), Query Frame = -1

Query:   97 DKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSY-DSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE-----------------KVVEIKPKINK---NWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYDI 627
            DKF  +WYQ+ S V I  + K +    +++ F + SV L    S  ++    FEL  EI PE SSY++ S K+E++L K   + W  +E                 KV    P  +K   +W++ +   + ELED+ DPLNSLFQKIY D +DETR+AMIKSFTES GTVLSTNW EV + +VE KPPDGMEYKKY++
Sbjct:    6 DKF--DWYQSDSDVCIGYMRKKLTDKDVRVEFEKQSVSLFLVTSSGDELLKKFELAHEIVPEKSSYRVSSMKIELRLKKSTDVKWSGIERKLEEQAEAAEAQDLVKKVAHTYPSSSKSTHDWNK-LEKQAAELEDEGDPLNSLFQKIYADASDETRRAMIKSFTESQGTVLSTNWGEVGKGKVEMKPPDGMEYKKYEL 200

[Close]

BLAST of SMED30021389 vs. TrEMBL
Match: A0A0R3W3N1 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS4547 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.156e-42
Identity = 87/194 (44.85%), Postives = 126/194 (64.95%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE--------KVVEI--------KPK-INK---NWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            +F+++WYQ+ S V + ++ K +D + L++ F E +V +  ++E     +   L  +I+P  SSYK  S K+E+KL K    NW  LE        KV+++        KPK +NK   +WD +    ++E+E+  DPLN+LFQKI+KD ND+TR+AMIKSFTES+GTVLSTNW EV + +VE KPPDGMEYK+Y
Sbjct:   17 RFRYQWYQSDSSVFVSVMRKGIDPATLRVTFAEDAVAIHINNE---LVNQLNLAHKIDPSKSSYKCTSMKIELKLAKTVSSNWSCLEGADPASSLKVIDVPVSSKDGDKPKKVNKTYHDWDAVAKE-ADEIEE-GDPLNNLFQKIFKDANDDTRRAMIKSFTESNGTVLSTNWDEVGKGKVEMKPPDGMEYKEY 205

[Close]

BLAST of SMED30021389 vs. TrEMBL
Match: A0A4S2LU02 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006090 PE=4 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 4.030e-42
Identity = 87/195 (44.62%), Postives = 120/195 (61.54%), Query Frame = -1

Query:   97 FSMNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIF-NETSVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE---------KVVEIKP---KINKNWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYDI 642
             S + DKF  +WYQ+   VTI  L KN+  S +++ F N+++         ++    F+L   I PE SS+++ S K+E+ + K+  I W  LE         KV    P   K+  +W+++    + ELEDD DPLN LFQ IY+D +DETR+AMIKSFTES GTVLSTNW EV + +VE KPPDGMEYKKY++
Sbjct:    1 MSASGDKF--DWYQSDRDVTINYLRKNLKPSDVRVDFDNQSAALFLLTPAGDELLKRFQLSHPIIPEQSSFRVSSMKIEVCVRKQTEIRWTQLEDVSTTDSVSKVAHSYPSSSKVAHDWNKLEKEAA-ELEDDGDPLNKLFQSIYRDASDETRRAMIKSFTESGGTVLSTNWDEVGKGKVEMKPPDGMEYKKYEL 192

[Close]

BLAST of SMED30021389 vs. TrEMBL
Match: A0A0X3NU62 (Suppressor of G2 allele of SKP1 homolog OS=Schistocephalus solidus OX=70667 GN=SUGT1 PE=4 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 7.358e-42
Identity = 89/206 (43.20%), Postives = 120/206 (58.25%), Query Frame = -1

Query:   97 FSMNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSY-DSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE--------KVVEIKP---------------KINKNWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYDI 642
              M  +  KH+WYQT + V I LL +N+D + L++ F+  SV +S  DS  +     F    EI+P +SSYK    KVE+K  K   + W  LE        K+ +  P               K   +WD++    ++E+E+  DPLN+LF KI+KD ND+T+KAMIKSFTES GTVLSTNW EV + +VE KPPDGMEY+KY I
Sbjct:   24 LRMEVESIKHDWYQTDNYVCISLLRRNLDPNALQVEFSPRSVRVSVSDSNGDHLLKAFSFSHEIDPNSSSYKCTPLKVELKAAKMSAVQWSCLEGEDPTASLKLPDTSPHQSMTSDKPTYPTSHKKASDWDKLAKE-ADEIEE-GDPLNNLFAKIFKDANDDTKKAMIKSFTESGGTVLSTNWDEVGKGKVEVKPPDGMEYRKYPI 227

[Close]

BLAST of SMED30021389 vs. Ensembl Cavefish
Match: sugt1 (SGT1 homolog, MIS12 kinetochore complex assembly cochaperone [Source:NCBI gene;Acc:103032285])

HSP 1 Score: 129.028 bits (323), Expect = 1.346e-35
Identity = 76/192 (39.58%), Postives = 107/192 (55.73%), Query Frame = -1

Query:  103 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSES-EKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE------KVVEIKPKIN---------KNWDQIMNGLSNELED----DNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 618
            KH+WYQT S VT+ +++KN     + + F E  +       S E +     L   + PE S+ KI STKVEIK+ K + I W  LE       V    P  N         +NWD+++  +  E +      +  LNSLFQ+IY DG+DE ++AM KSF ES GTVLSTNW +V +++V+  PPD ME+K+Y
Sbjct:  135 KHDWYQTESQVTVTIMVKNAKKEDVAVTFGEKELRAEMKLPSGEDYCLHIHLLHPVVPEQSTLKILSTKVEIKMKKTEAIRWEKLEGEGTLPSVKHFAPSPNQYPSSSHYTRNWDKLVGDIKEEEKSEKQEGDAALNSLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWLDVGKRKVDVNPPDDMEWKQY 326

[Close]

BLAST of SMED30021389 vs. Ensembl Cavefish
Match: chordc1a (cysteine and histidine-rich domain-containing protein 1 [Source:NCBI gene;Acc:103041654])

HSP 1 Score: 49.6766 bits (117), Expect = 7.738e-7
Identity = 28/86 (32.56%), Postives = 46/86 (53.49%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESEK-FSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            + +W+QT   VTI +  KN +  L  +  N T+V +    E EK F     L+G I+   S   I ++K+EI + K +P++W  L+
Sbjct:  231 RFDWHQTGGQVTISIYAKNCNPELCSVQANSTTVCVRVVFEVEKEFEQKLSLWGVIDVSKSVVNIMASKIEISMKKAEPMSWARLD 316

[Close]

BLAST of SMED30021389 vs. Ensembl Cavefish
Match: chordc1a (cysteine and histidine-rich domain-containing protein 1 [Source:NCBI gene;Acc:103041654])

HSP 1 Score: 49.2914 bits (116), Expect = 9.705e-7
Identity = 28/86 (32.56%), Postives = 46/86 (53.49%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESEK-FSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            + +W+QT   VTI +  KN +  L  +  N T+V +    E EK F     L+G I+   S   I ++K+EI + K +P++W  L+
Sbjct:  224 RFDWHQTGGQVTISIYAKNCNPELCSVQANSTTVCVRVVFEVEKEFEQKLSLWGVIDVSKSVVNIMASKIEISMKKAEPMSWARLD 309

[Close]

BLAST of SMED30021389 vs. Ensembl Yeast
Match: SGT1 (Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000005583])

HSP 1 Score: 89.3521 bits (220), Expect = 1.998e-21
Identity = 65/212 (30.66%), Postives = 104/212 (49.06%), Query Frame = -1

Query:  103 KFKHEWYQTPSVVTICLLIKNVDSSLLKI-IF----NETSVELSYD--SESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKPKI----NKNWDQIMNGLS---------------------------NELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 624
            KFK +WYQ+ + VTI L   N+  S  ++ I+    +  ++ +SY       +F +  +L  E++P+  S KI+  K+EI L K D   W  LE+ +  +        KN D     LS                            E +++    +S FQK+Y   + +T++AM+KSF ES+GT LST+W +V +  V+T PP+GME K +
Sbjct:  184 KFKIDWYQSSTSVTISLFTVNLPESKEQVNIYISPNDRRTLSISYQVPKSGSEFQYNAKLSHEVDPKAVSLKIFPKKLEITLSKIDSTQWKKLEEDILTESSRLSDEGKNSDSATRLLSAETASKERLSYPSSSKKKIDWSKLDIDEEADEEAGSADSFFQKLYAGADPDTKRAMMKSFIESNGTALSTDWEDVSKGTVKTSPPEGMEPKHW 395

[Close]

BLAST of SMED30021389 vs. Ensembl Nematostella
Match: EDO40460 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S726])

HSP 1 Score: 115.161 bits (287), Expect = 6.118e-31
Identity = 72/208 (34.62%), Postives = 114/208 (54.81%), Query Frame = -1

Query:  100 EFSMNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSES-EKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEI-KPKIN--------------------KNWDQIMNGLSNELEDDND----PLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYD 645
            + S    K  ++WYQT + V + ++IKN     + I + +  + ++    S   +S   +L   ++P     KI STK+E+K+ K + I W +LE    + KP +                     K+WD++   ++ E E +       LN LFQKIY +G+DE ++AM KSF ES GTVLSTNW+EV +++VE KPPDGME+K+++
Sbjct:  102 QCSARVYKHVYDWYQTETHVVVSVMIKNSKQEDVYIEYGDQHLSVTVRLPSGNDYSLELDLAHPVSPSQCKTKILSTKIELKIKKLEAIRWSSLETDHNVTKPAVKFPQQNATADPHQYPSSRHVVKDWDKLAAEVAKEDEAEKQEGEAALNQLFQKIYGEGSDEVKQAMNKSFIESGGTVLSTNWAEVGKEKVEVKPPDGMEWKEWE 309

[Close]

BLAST of SMED30021389 vs. Ensembl Nematostella
Match: EDO42059 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2K5])

HSP 1 Score: 46.595 bits (109), Expect = 2.696e-6
Identity = 31/86 (36.05%), Postives = 44/86 (51.16%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYD-SESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            +++W+Q  + V I +  K  D +   I  N T +  S    E+  F     L+G I+PE S   +  TKVEIKL K D  +W NLE
Sbjct:  231 RYDWFQMGNSVVISVYAKLTDPNATVIKANSTQLSASIIFGEASSFDLEISLYGVIDPEKSEVLMSPTKVEIKLRKADIGSWSNLE 316

[Close]

BLAST of SMED30021389 vs. Ensembl Medaka
Match: sugt1 (SGT1 homolog, MIS12 kinetochore complex assembly cochaperone [Source:NCBI gene;Acc:101158053])

HSP 1 Score: 121.709 bits (304), Expect = 8.228e-33
Identity = 76/192 (39.58%), Postives = 110/192 (57.29%), Query Frame = -1

Query:  103 KHEWYQTPSVVTICLLIKNV--DSSLLKIIFNETSVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEK------VVEIKP-------KINKNWDQIMNGLSNE-----LEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKY 618
            K++WYQT S V + ++ KNV  DS  +  +  E S E+      E ++    L   I P+ SS+K+ +TKVEIK+ K + I W  LE       +    P          + WD++++ +S E     LE D   LN LFQ+IY DG+DE ++AM KSF ES GTVLSTNW +V +++VE  PPD +E++KY
Sbjct:  135 KYDWYQTESQVIVTVMAKNVPKDSVSVNFVEKELSAEMQLPC-GENYNLHLHLLHPILPQQSSFKVLTTKVEIKMKKTEAIRWEKLEGEGQQTNIKHFNPDPYPTSLHYTRKWDKMVSDISEEEKNEKLEGDA-ALNKLFQQIYTDGSDEVKRAMNKSFMESAGTVLSTNWKDVGKRKVEASPPDDVEHRKY 324

[Close]

BLAST of SMED30021389 vs. Ensembl Medaka
Match: zgc:92429 (cysteine and histidine-rich domain-containing protein 1 [Source:NCBI gene;Acc:101164846])

HSP 1 Score: 53.1434 bits (126), Expect = 6.302e-8
Identity = 31/87 (35.63%), Postives = 47/87 (54.02%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            +H+W+QT + V I +  KN       I  N+T  S ++ +D E + F   F L+G I+   SS  +  +KVEI L K D ++W  LE
Sbjct:  227 RHDWHQTRNAVVITIYAKNALPEFCTIEANQTVLSCQIQFDRE-KIFKRNFHLWGVIDVNKSSVNLVPSKVEISLHKADQVSWGKLE 312

[Close]

BLAST of SMED30021389 vs. Ensembl Medaka
Match: zgc:92429 (cysteine and histidine-rich domain-containing protein 1 [Source:NCBI gene;Acc:101164846])

HSP 1 Score: 52.7582 bits (125), Expect = 6.699e-8
Identity = 31/87 (35.63%), Postives = 47/87 (54.02%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNVDSSLLKIIFNET--SVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            +H+W+QT + V I +  KN       I  N+T  S ++ +D E + F   F L+G I+   SS  +  +KVEI L K D ++W  LE
Sbjct:  207 RHDWHQTRNAVVITIYAKNALPEFCTIEANQTVLSCQIQFDRE-KIFKRNFHLWGVIDVNKSSVNLVPSKVEISLHKADQVSWGKLE 292

[Close]

BLAST of SMED30021389 vs. Planmine SMEST
Match: SMESG000011789.1 (SMESG000011789.1)

HSP 1 Score: 360.533 bits (924), Expect = 1.463e-128
Identity = 179/180 (99.44%), Postives = 180/180 (100.00%), Query Frame = -1

Query:   97 MNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKPKINKNWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYDI 636
            MNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSY+SESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKPKINKNWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYDI
Sbjct:    1 MNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYNSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKPKINKNWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHGTVLSTNWSEVKEKQVETKPPDGMEYKKYDI 180

[Close]

BLAST of SMED30021389 vs. Planmine SMEST
Match: SMESG000019808.1 (SMESG000019808.1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.165e-7
Identity = 33/87 (37.93%), Postives = 46/87 (52.87%), Query Frame = -1

Query:  364 KHEWYQTPSVVTICLLIKNV--DSSLLKIIFNETSVELSYDSESEKFSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLE 618
            + +W+QT S VTI L  K V  +S  +K    + SV   ++  S  +   + LF EI+P  S  KI   KVE+ L KK   NW+ LE
Sbjct:  215 RSDWFQTGSSVTISLFSKLVLPESVTIKANAVKLSVAFKFECGSGYYEKLYNLFEEIDPSQSQVKISCAKVEVILKKKHFTNWVALE 301

The following BLAST results are available for this feature:

BLAST of SMED30021389 vs. Ensembl Human
BLAST of SMED30021389 vs. Ensembl Celegans
BLAST of SMED30021389 vs. Ensembl Fly
BLAST of SMED30021389 vs. Ensembl Zebrafish
BLAST of SMED30021389 vs. Ensembl Xenopus
BLAST of SMED30021389 vs. Ensembl Mouse
BLAST of SMED30021389 vs. UniProt/SwissProt
BLAST of SMED30021389 vs. TrEMBL
BLAST of SMED30021389 vs. Ensembl Cavefish
BLAST of SMED30021389 vs. Ensembl Sea Lamprey
BLAST of SMED30021389 vs. Ensembl Yeast
BLAST of SMED30021389 vs. Ensembl Nematostella
BLAST of SMED30021389 vs. Ensembl Medaka
BLAST of SMED30021389 vs. Planmine SMEST

BLAST of SMED30021389 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SUGT1	4.121e-36	41.03	SGT1 homolog, MIS12 kinetochore complex assembly c...	[more]
SUGT1	8.174e-36	41.03	SGT1 homolog, MIS12 kinetochore complex assembly c...	[more]
CHORDC1	4.793e-8	33.33	cysteine and histidine rich domain containing 1 [S...	[more]
CHORDC1	4.793e-8	33.33	cysteine and histidine rich domain containing 1 [S...	[more]
CHORDC1	4.793e-8	33.33	cysteine and histidine rich domain containing 1 [S...	[more]

BLAST of SMED30021389 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
D1054.3	1.622e-28	32.84	pep chromosome:WBcel235:V:10771451:10772832:1 gene...	[more]
D1054.3	5.472e-19	56.36	pep chromosome:WBcel235:V:10771551:10772832:1 gene...	[more]

BLAST of SMED30021389 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Sgt1	4.977e-34	39.11	gene:FBgn0265101 transcript:FBtr0081862	[more]
CHORD	4.614e-8	33.71	gene:FBgn0029503 transcript:FBtr0084554	[more]

BLAST of SMED30021389 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
sugt1	1.398e-33	39.79	SGT1 homolog, MIS12 kinetochore complex assembly c...	[more]
sugt1	2.060e-33	39.79	SGT1 homolog, MIS12 kinetochore complex assembly c...	[more]

BLAST of SMED30021389 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pnpla3	5.505e-38	42.56	patatin like phospholipase domain containing 3 [So...	[more]
pnpla3	7.858e-38	42.56	patatin like phospholipase domain containing 3 [So...	[more]
itgb1bp2	1.350e-9	34.62	lymphoid-restricted membrane protein-like [Source:...	[more]
itgb1bp2	1.704e-9	34.62	lymphoid-restricted membrane protein-like [Source:...	[more]
itgb1bp2	1.984e-9	34.62	lymphoid-restricted membrane protein-like [Source:...	[more]

BLAST of SMED30021389 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Sugt1	4.384e-35	40.00	SGT1, suppressor of G2 allele of SKP1 (S. cerevisi...	[more]
Chordc1	7.581e-7	33.33	cysteine and histidine-rich domain (CHORD)-contain...	[more]

BLAST of SMED30021389 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9Y2Z0\|SGT1_HUMAN	3.925e-35	41.03	Protein SGT1 homolog OS=Homo sapiens OX=9606 GN=SU...	[more]
sp\|Q2KIK0\|SGT1_BOVIN	7.525e-35	40.51	Protein SGT1 homolog OS=Bos taurus OX=9913 GN=SUGT...	[more]
sp\|Q9CX34\|SGT1_MOUSE	3.067e-34	40.00	Protein SGT1 homolog OS=Mus musculus OX=10090 GN=S...	[more]
sp\|B0BN85\|SGT1_RAT	3.165e-34	40.00	Protein SGT1 homolog OS=Rattus norvegicus OX=10116...	[more]
sp\|Q9SUR9\|SGT1A_ARATH	9.675e-32	36.00	Protein SGT1 homolog A OS=Arabidopsis thaliana OX=...	[more]

BLAST of SMED30021389 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1B6CNN1	5.918e-45	47.03	Uncharacterized protein OS=Clastoptera arizonana O...	[more]
A0A4E0QY98	1.096e-42	44.95	Suppressor of G2 allele of SKP1 OS=Fasciola hepati...	[more]
A0A0R3W3N1	1.156e-42	44.85	Uncharacterized protein OS=Taenia asiatica OX=6051...	[more]
A0A4S2LU02	4.030e-42	44.62	Uncharacterized protein OS=Opisthorchis felineus O...	[more]
A0A0X3NU62	7.358e-42	43.20	Suppressor of G2 allele of SKP1 homolog OS=Schisto...	[more]

BLAST of SMED30021389 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
sugt1	1.346e-35	39.58	SGT1 homolog, MIS12 kinetochore complex assembly c...	[more]
chordc1a	7.738e-7	32.56	cysteine and histidine-rich domain-containing prot...	[more]
chordc1a	9.705e-7	32.56	cysteine and histidine-rich domain-containing prot...	[more]

BLAST of SMED30021389 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021389 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
SGT1	1.998e-21	30.66	Cochaperone protein; regulates activity of adenyly...	[more]

BLAST of SMED30021389 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO40460	6.118e-31	34.62	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42059	2.696e-6	36.05	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30021389 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
sugt1	8.228e-33	39.58	SGT1 homolog, MIS12 kinetochore complex assembly c...	[more]
zgc:92429	6.302e-8	35.63	cysteine and histidine-rich domain-containing prot...	[more]
zgc:92429	6.699e-8	35.63	cysteine and histidine-rich domain-containing prot...	[more]

BLAST of SMED30021389 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000011789.1	1.463e-128	99.44	SMESG000011789.1	[more]
SMESG000019808.1	1.165e-7	37.93	SMESG000019808.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30021389 ID=SMED30021389|Name=SMED30021389|organism=Schmidtea mediterranea sexual|type=transcript|length=678bp
TTATAATTTTATCGTTAGTAAACCTTTCATTTTCCTCTAAAATAACAATA
CTTATGAAGTCAAATTGGCTAAAAACTCAGTTTATTAAACAAATCATATA
TCATATTTTTTATATTCCATTCCATCTGGAGGTTTGGTTTCGACTTGTTT
TTCTTTAACCTCAGACCAATTAGTACTTAAAACAGTTCCGTGGGATTCAG
TAAAACTCTTAATCATAGCTTTTCTTGTTTCATCATTGCCATCTTTGTAT
ATTTTTTGAAACAATGAATTTAATGGATCATTATCGTCTTCTAATTCATT
TGACAAACCATTCATAATTTGGTCCCAATTTTTATTAATCTTTGGCTTAA
TTTCAACCACTTTTTCTAAATTTATCCAATTAATGGGATCTTTTTTTGGT
AATTTTATTTCAACTTTAGTCGAATATATTTTGTATGAAGAATTTTCCGG
ATTTATTTCTCCAAACAATTCAAAAGTATGACTAAATTTTTCTGATTCGC
TATCATATGACAGCTCAACTGAGGTCTCATTAAATATGATTTTGAGCAAT
GAGGAATCAACATTTTTAATCAATAAACAAATAGTCACTACTGAAGGCGT
TTGATACCATTCATGTTTGAATTTATCGAAATTCATTGAAAACTCTAACG
TAAACAAGATAATTGATTAAAAATGTTG

protein sequence of SMED30021389-orf-1

>SMED30021389-orf-1 ID=SMED30021389-orf-1|Name=SMED30021389-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=181bp
MNFDKFKHEWYQTPSVVTICLLIKNVDSSLLKIIFNETSVELSYDSESEK
FSHTFELFGEINPENSSYKIYSTKVEIKLPKKDPINWINLEKVVEIKPKI
NKNWDQIMNGLSNELEDDNDPLNSLFQKIYKDGNDETRKAMIKSFTESHG
TVLSTNWSEVKEKQVETKPPDGMEYKKYDI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000074	oocyte
PLANA:0000204	ovary
PLANA:0000208	testis
PLANA:0000231	vitelline gland
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR007052	CS_dom
IPR007699	SGS_dom
IPR008978	HSP20-like_chaperone

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007699	SGS domain	PFAM	PF05002	SGS	coord: 102..178 e-value: 5.2E-31 score: 106.6
IPR007699	SGS domain	PROSITE	PS51048	SGS	coord: 93..180 score: 28.262
IPR007052	CS domain	PFAM	PF04969	CS	coord: 7..81 e-value: 7.2E-15 score: 55.8
IPR007052	CS domain	PROSITE	PS51203	CS	coord: 3..91 score: 17.161
IPR008978	HSP20-like chaperone	GENE3D	G3DSA:2.60.40.790		coord: 4..93 e-value: 2.1E-25 score: 91.1
IPR008978	HSP20-like chaperone	SUPERFAMILY	SSF49764	HSP20-like chaperones	coord: 5..158
None	No IPR available	PANTHER	PTHR45862	FAMILY NOT NAMED	coord: 5..178
None	No IPR available	PANTHER	PTHR45862:SF1	PROTEIN SGT1 HOMOLOG	coord: 5..178
None	No IPR available	CDD	cd06466	p23_CS_SGT1_like	coord: 9..91 e-value: 2.36178E-26 score: 93.8055

Main menu

SMED30021389

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 17

Owlery

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