MFS domain-containing protein

Overview
NameMFS domain-containing protein
Smed IDSMED30021377
Length (bp)1258
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of MFS domain-containing protein (SMED30021377) t-SNE clustered cells

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30021377) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MFS domain-containing protein (SMED30021377) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30021377) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021377

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30021377SMESG000062462.1 dd_Smed_v4_14825_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021377SMESG000062462.1 dd_Smed_v4_14825_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30021377SMESG000062462.1 dd_Smed_v4_14825_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30021377SMESG000062462.1 dd_Smed_v4_14825_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: Y59A8B.21 (pep chromosome:WBcel235:V:17986260:17994546:1 gene:WBGene00013353.1 transcript:Y59A8B.21.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y59A8B.21)

HSP 1 Score: 99.7525 bits (247), Expect = 6.900e-32
Identity = 52/124 (41.94%), Postives = 75/124 (60.48%), Query Frame = 3 
Query:  348 SVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTP 719
            SV L+ + I+    L  + YG+LFG+G GIAY  A+S AINW P++ G++ GIV +GFGL S  F  IQT+ +NP NL        + YF   ++L RVP  F  +  +YGIMQ +  +++  P
Sbjct:  108 SVALSAYAIQHSFALFFIVYGLLFGLGSGIAYVTAVSTAINWAPDKIGVISGIVAAGFGLSSSIFAPIQTMIVNPLNLPAT----KDGYFLQPELLHRVPALFTKLAVIYGIMQAMALIVVCDP 227

HSP 2 Score: 56.6102 bits (135), Expect = 6.900e-32
Identity = 33/82 (40.24%), Postives = 47/82 (57.32%), Query Frame = 2 
Query:   56 LCVL--GGFLIHFSLGTIYTFGNMSPYIASYMKFICNFNVTNGDMLWIFAGGLTFQG---ITMSLGGMLSKKIGTRLTVLIG 286
            +CV+  G   IH ++GT +TFGNM PY+ASYM+   + +V    M+WI     TFQG     M +GG+ S     R++  IG
Sbjct:   24 ICVILFGAVSIHLTIGTYHTFGNMLPYMASYMRNFTDPSVRIEHMMWI----PTFQGCFPFAMVIGGLTSSTFSPRVSAFIG 101

HSP 3 Score: 57.3806 bits (137), Expect = 1.010e-8
Identity = 39/160 (24.38%), Postives = 80/160 (50.00%), Query Frame = 3 
Query:  708 LFTPISQTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFIL 1187
            +F  + +T+G+ FI+ D ++++  S+ S+ N+  RI WG + D+ S++ A+       T L   F   + +T ++  ++  L W+    + +    A+   A  K FG ++ +  +G L  + + + I+ A  +   +  IGY  +F + +   F AFI 
Sbjct:  292 MFYNLYKTYGESFIEDDMFMAMAFSIASVANAIARIGWGYLTDRTSFQIALS----TATCLASVFLLTMPMTREL-GKMAFLFWLVGTFICMGATHALFITATVKCFGNRHKANNYGYLILSTTTSGILLAAISQFYLKAIGYTYLFIITSIFPFCAFIF 446
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 98.9821 bits (245), Expect = 1.092e-27
Identity = 90/352 (25.57%), Postives = 159/352 (45.17%), Query Frame = 3 
Query:  360 TYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGC----------------------------------------LLLFTPIS-------------QTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNESL*ML*TNKIRN*MAD 1256
            T+++I+      +LT G +  +G  IAYS  +  A  W P+  GL  GI++ G+G G+   + +QT FINP +   N     + +F   D+L+RVP  F+++   + + Q +G                                         L+LF  ++             + +GQ FI SD++LS++GS+ SIFN+  R+ WG I D  SY+ ++ ++ ++  +L     F + +   +   ++ L  +C +   I G F+I P    K F   N S ++G L  A S+A +I  L ++  +S+IG+  +F +       +  LTF      L  L  +  R  + +
Sbjct:  119 TFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVN----DQGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPEGDIHVETENLIGTNSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLA----FSMPLVKMVSNDVLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLISTHALSEIGFEYLFIVCGCFMLTSLFLTFIVHKTDLAQLLVSAEREHLEE 462

HSP 2 Score: 43.5134 bits (101), Expect = 1.092e-27
Identity = 24/78 (30.77%), Postives = 44/78 (56.41%), Query Frame = 2 
Query:   62 VLGGFLIHFSLGTIYTFGNMSPYIASYMKFICNFNVTNGDMLWI--FAGGLTFQGITMSLGGMLSKKIGTRLTVLIGS 289
            ++GG +   + G +YT GN+ PY+ SY ++    ++  G ++W+     GL    + M +GG++ +K G R   L+GS
Sbjct:   35 LVGGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRTGQLIWLQTLLSGLP---MGMVIGGLVERKFGGRAGALLGS 109
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 98.5969 bits (244), Expect = 1.399e-27
Identity = 90/352 (25.57%), Postives = 159/352 (45.17%), Query Frame = 3 
Query:  360 TYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGC----------------------------------------LLLFTPIS-------------QTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNESL*ML*TNKIRN*MAD 1256
            T+++I+      +LT G +  +G  IAYS  +  A  W P+  GL  GI++ G+G G+   + +QT FINP +   N     + +F   D+L+RVP  F+++   + + Q +G                                         L+LF  ++             + +GQ FI SD++LS++GS+ SIFN+  R+ WG I D  SY+ ++ ++ ++  +L     F + +   +   ++ L  +C +   I G F+I P    K F   N S ++G L  A S+A +I  L ++  +S+IG+  +F +       +  LTF      L  L  +  R  + +
Sbjct:  105 TFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVN----DQGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPEGDIHVETENLIGTNSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLA----FSMPLVKMVSNDVLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLISTHALSEIGFEYLFIVCGCFMLTSLFLTFIVHKTDLAQLLVSAEREHLEE 448

HSP 2 Score: 43.5134 bits (101), Expect = 1.399e-27
Identity = 24/78 (30.77%), Postives = 44/78 (56.41%), Query Frame = 2 
Query:   62 VLGGFLIHFSLGTIYTFGNMSPYIASYMKFICNFNVTNGDMLWI--FAGGLTFQGITMSLGGMLSKKIGTRLTVLIGS 289
            ++GG +   + G +YT GN+ PY+ SY ++    ++  G ++W+     GL    + M +GG++ +K G R   L+GS
Sbjct:   21 LVGGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRTGQLIWLQTLLSGLP---MGMVIGGLVERKFGGRAGALLGS 95
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693542:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 98.5969 bits (244), Expect = 1.399e-27
Identity = 90/352 (25.57%), Postives = 159/352 (45.17%), Query Frame = 3 
Query:  360 TYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGC----------------------------------------LLLFTPIS-------------QTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNESL*ML*TNKIRN*MAD 1256
            T+++I+      +LT G +  +G  IAYS  +  A  W P+  GL  GI++ G+G G+   + +QT FINP +   N     + +F   D+L+RVP  F+++   + + Q +G                                         L+LF  ++             + +GQ FI SD++LS++GS+ SIFN+  R+ WG I D  SY+ ++ ++ ++  +L     F + +   +   ++ L  +C +   I G F+I P    K F   N S ++G L  A S+A +I  L ++  +S+IG+  +F +       +  LTF      L  L  +  R  + +
Sbjct:  105 TFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVN----DQGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPEGDIHVETENLIGTNSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLA----FSMPLVKMVSNDVLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLISTHALSEIGFEYLFIVCGCFMLTSLFLTFIVHKTDLAQLLVSAEREHLEE 448

HSP 2 Score: 43.5134 bits (101), Expect = 1.399e-27
Identity = 24/78 (30.77%), Postives = 44/78 (56.41%), Query Frame = 2 
Query:   62 VLGGFLIHFSLGTIYTFGNMSPYIASYMKFICNFNVTNGDMLWI--FAGGLTFQGITMSLGGMLSKKIGTRLTVLIGS 289
            ++GG +   + G +YT GN+ PY+ SY ++    ++  G ++W+     GL    + M +GG++ +K G R   L+GS
Sbjct:   21 LVGGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRTGQLIWLQTLLSGLP---MGMVIGGLVERKFGGRAGALLGS 95
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|P37662|YHJX_ECOLI (Uncharacterized MFS-type transporter YhjX OS=Escherichia coli (strain K12) OX=83333 GN=yhjX PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 3.694e-7
Identity = 61/282 (21.63%), Postives = 116/282 (41.13%), Query Frame = 3 
Query:  375 KSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNL------------------------SPNITIETERYFDDRDML----DRVPKCFLLIGGMYGIMQLLGCL--LLFTPISQTFGQLFIKSDYYLSVVGSVG-----SIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSI 1115
              +L ++ L+ GVL G+  G  Y + +S  + W P ++GL+    +  +GLGS+ F  I T  +    L                        +P   ++T     ++D       R P+ ++L      +M L  C+  L    +++   Q    S  +L VV +       SI N  GR++ G + DK +    + +   ++ + M    F  L      A       I  +  N  G   + P+ +++ FG+ N++  +G+++    I SI G++  S+
Sbjct:   96 SDNLMMLWLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLETVGLEKTFVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWML-----AVMFLTACMSGLYVIGVAKDIAQ----SLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGMAALLFAPLNAVTFFAA------IACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGSICGSIIASL 362
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A0H3YF06 (Slc16a-5 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc16a-5 PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 1.162e-68
Identity = 83/131 (63.36%), Postives = 110/131 (83.97%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIE-TERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPISQT 731
            S SV+LTYFTI++++Y+ ILTYGV+FG G+GIAYSV ++A+I W PN +GLVIG+VV+GFGLGS+AFNQIQTL+INP N+SPN T E T+RYF D  +L++VP CFL  G +Y  +QL+GC++LF+P SQT
Sbjct:   96 SASVILTYFTIQANIYVTILTYGVIFGFGVGIAYSVVLAASIKWFPNNKGLVIGVVVAGFGLGSLAFNQIQTLYINPQNISPNFTKEDTDRYFTDEKVLEKVPYCFLYTGIIYASIQLIGCIILFSP-SQT 225

HSP 2 Score: 114.39 bits (285), Expect = 1.162e-68
Identity = 57/89 (64.04%), Postives = 69/89 (77.53%), Query Frame = 2 
Query:   35 LTNYKGVLCVLGGFLIHFSLGTIYTFGNMSPYIASYMK-FICNFNVTNGDMLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSWTM 298
            + N+KG LC+LGGFLIHFSLG+IYTFGN+SPYIASYM+      +V N DM+WIFA  L  QG+TMS GG+L KK+G+R TVLIG   M
Sbjct:    7 IRNFKGYLCILGGFLIHFSLGSIYTFGNISPYIASYMRSRPVTQSVKNSDMVWIFAFALASQGLTMSFGGVLDKKVGSRFTVLIGCCIM 95

HSP 3 Score: 195.667 bits (496), Expect = 7.974e-54
Identity = 113/248 (45.56%), Postives = 163/248 (65.73%), Query Frame = 3 
Query:  477 PNQRGLVIGIVV-SGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLF-TPISQTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNESL 1214
            P+Q  ++I I+  +G     ++ N I   + + NN+   +  +      D ++   + +    I  ++ I    G + ++ T + +TFGQLFI SDYYL++VGSV SIFN+ GRI WG+++D++SYK A+R +   +TILMGTFY C L+ N I A+++ L+W+C I  NICGIFA+MPA  AKLFG+K V TI+G+LF+AN+ A I GALTTSI +SKI Y GIF +GAG+T +A ILTF H    L
Sbjct:  222 PSQTEMMISILSNTGSSNSELSTNFISETYDDENNIQSLLPKDNH----DSNIFKAIKQRDFWI--LWFIFMFCGTINVYATSLYKTFGQLFITSDYYLALVGSVASIFNAMGRIFWGQVMDRFSYKVAIRSIIINSTILMGTFYLCHLVPNSIGARVMFLIWVCGIFANICGIFAVMPAITAKLFGLKKVVTIYGILFSANAFAGIFGALTTSIFLSKINYFGIFGIGAGLTTIALILTFLHKRIDL 463
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A3S1CE33 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_002051 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 3.235e-63
Identity = 106/296 (35.81%), Postives = 175/296 (59.12%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITI--ETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLL------LFTPISQTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIV---AQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFF 1196
            SF V++TYFTI+   +L ++TYG+LFG GIGI+Y++ +S  + W P ++GLV G+VV+GFG G+  FNQ+QT FINP+NL   + +  E+       D+++  P    +I   Y  M     LL        + + + +GQ FI  D +L+VVGS  +IFN+ GRI WG + DK S++ ++ +            + CL++T Q+     + +  +W+C +  +  G F+++P A AK FG + VST +G++FT+  I++ +GA  +  LIS+IG+ G+  M +  +F + +L  F
Sbjct:   95 SFGVLVTYFTIEISFFLTVVTYGLLFGAGIGISYTMPLSCGMKWFPERKGLVNGLVVAGFGGGAFIFNQVQTAFINPDNLPAELDVGNESNVLCKGEDVVNVGP--LEMIKSKYFYMLWFIFLLNGQGINFVSTLYKAYGQTFIDDDNFLAVVGSFAAIFNAGGRIWWGFLADKTSFRVSMMVACAC--------FTCLILTFQVTELQGREMFFLWVCLLFGSFSGNFSLLPTATAKCFGREYVSTNYGLVFTSQVISAPLGAFLSQQLISQIGWPGLCFMVSSFSFTSMMLAIF 380

HSP 2 Score: 91.2781 bits (225), Expect = 3.235e-63
Identity = 43/88 (48.86%), Postives = 61/88 (69.32%), Query Frame = 2 
Query:   38 TNYKGVLCVLGGFLIHFSLGTIYTFGNMSPYIASYMKFI-CNFNVTNGDMLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSWTM 298
            + ++GVL V GG L+H SLGT+YTFGN++PY+ SYM++     N+ N +  W+FA     QG+ MSL G++ KK+G RLT L+G W M
Sbjct:    7 SKWRGVLVVAGGILVHLSLGTLYTFGNLNPYLTSYMRWTGTAPNLKNTECTWVFAAAAIGQGLVMSLSGLIQKKLGPRLTCLLGGWLM 94
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: K1QXL9 (Translationally-controlled tumor-like protein OS=Crassostrea gigas OX=29159 GN=CGI_10011187 PE=3 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 9.212e-63
Identity = 109/289 (37.72%), Postives = 166/289 (57.44%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNIT---IETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLF--------TPISQTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFL 1175
            S  V LTYFTIK   Y  + TYG +FGIG+GIAY+  +  A+ WLP++ GLV G VV+GFG G+  F+QIQT +INP+N   N        ER    R+ L R P+ + L            C+ LF        + + + FGQ FI  DY+L+VVGS+ ++ N  GRIMWG + D+Y +KTA+     + +  + +F+        +  + ++L+W+C + ++  G F+++P A AK FG  +    +GM+FTA+ I+S I AL TS L + + + G+F M A  +F+
Sbjct:  127 SAGVALTYFTIKVSFYWTLFTYGFVFGIGVGIAYATPMGCAMKWLPDKEGLVNGCVVAGFGGGAFIFDQIQTAYINPHNEKANFDENGENEERTLTPRETL-RQPEFYKLW-----------CMYLFNGQGISFISSLYKAFGQTFITDDYFLAVVGSLAAVCNGAGRIMWGALADRYCFKTAM-----VFSTGLFSFFILSFELTSLAGKSLYLIWVCCLFLSFSGNFSLLPTATAKAFGKTHYGVNYGMVFTASVISSPISALLTSKLKAVVHWYGMFYMVAFFSFV 398

HSP 2 Score: 83.1889 bits (204), Expect = 9.212e-63
Identity = 39/84 (46.43%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:   44 YKGVLCVLGGFLIHFSLGTIYTFGNMSPYIASYMK-FICNFNVTNGDMLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSW 292
            ++G   VLGG LIH +LGTIYTFGNM+PY+ SYMK    + ++   +  WI A     QG++M LGG++ + +G +L+ LIG+W
Sbjct:   41 WRGYTVVLGGVLIHLTLGTIYTFGNMTPYMTSYMKRHNVDVSLNYAESTWISAIAAMGQGMSMFLGGIMYRALGPKLSTLIGAW 124
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8H205 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.222e-56
Identity = 70/129 (54.26%), Postives = 100/129 (77.52%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITI-ETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPIS 725
            S  V+L+YF +KSHL L+++TYG+LFG+G GIAY+VAI+A+++W P++RGLV GIVV+GFG GS+ F+Q QTL+INP+N+  N+T    ERYFD   ++DRVP CF+ +G +Y +MQ +G  LL  P +
Sbjct:   91 SLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTA 219

HSP 2 Score: 96.6709 bits (239), Expect = 1.222e-56
Identity = 46/88 (52.27%), Postives = 62/88 (70.45%), Query Frame = 2 
Query:   38 TNYKGVLCVLGGFLIHFSLGTIYTFGNMSPYIASYMKFICNFN-VTNGDMLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSWTM 298
            T +KG+  V+GGFLIHF+LGT+YTFGNMSPY+ SYM+   +   +   D  WIF   +  QG +M+ GG++ K+IG RLT L+GSW M
Sbjct:    3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIM 90

HSP 3 Score: 101.679 bits (252), Expect = 2.315e-19
Identity = 55/157 (35.03%), Postives = 96/157 (61.15%), Query Frame = 3 
Query:  726 QTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIV--AQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILT 1190
            ++FG     +D+YL++VGS+ + FN+  R+MWG + D+ +Y+           + + +F   +L++   +  A   +L+W+C++     G FA+ PAA AKL+G  N++  +G+LFTAN+ + IIGAL T++L   IG+LG+F +GA  + L  + T
Sbjct:  295 KSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA---------LMFVLSFGMAVLMSTFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLKDAIGWLGLFLLGAAFSTLGAVFT 442

HSP 4 Score: 101.679 bits (252), Expect = 2.315e-19
Identity = 55/157 (35.03%), Postives = 96/157 (61.15%), Query Frame = 3 
Query:  726 QTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIV--AQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILT 1190
            ++FG     +D+YL++VGS+ + FN+  R+MWG + D+ +Y+           + + +F   +L++   +  A   +L+W+C++     G FA+ PAA AKL+G  N++  +G+LFTAN+ + IIGAL T++L   IG+LG+F +GA  + L  + T
Sbjct:  527 KSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA---------LMFVLSFGMAVLMSTFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLKDAIGWLGLFLLGAAFSTLGAVFT 674
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8HL50 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 1.626e-56
Identity = 70/129 (54.26%), Postives = 100/129 (77.52%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITI-ETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPIS 725
            S  V+L+YF +KSHL L+++TYG+LFG+G GIAY+VAI+A+++W P++RGLV GIVV+GFG GS+ F+Q QTL+INP+N+  N+T    ERYFD   ++DRVP CF+ +G +Y +MQ +G  LL  P +
Sbjct:   91 SLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTA 219

HSP 2 Score: 96.2857 bits (238), Expect = 1.626e-56
Identity = 46/88 (52.27%), Postives = 62/88 (70.45%), Query Frame = 2 
Query:   38 TNYKGVLCVLGGFLIHFSLGTIYTFGNMSPYIASYMKFICNFN-VTNGDMLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSWTM 298
            T +KG+  V+GGFLIHF+LGT+YTFGNMSPY+ SYM+   +   +   D  WIF   +  QG +M+ GG++ K+IG RLT L+GSW M
Sbjct:    3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIM 90

HSP 3 Score: 102.834 bits (255), Expect = 3.889e-20
Identity = 66/222 (29.73%), Postives = 118/222 (53.15%), Query Frame = 3 
Query:  552 QTLFINPNNLSPNITIETERYFDDRDMLD-------RVPKCFLLIGGMYGIMQLLGCLLLFTPISQTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIV--AQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILT 1190
            Q   ++  + S  + +ET  + +    LD       R P+ +LL        Q    ++      ++FG     +D+YL++VGS+ + FN+  R+MWG + D+ +Y+           + + +F   +L++   +  A   +L+W+C++     G FA+ PAA AKL+G  N++  +G+LFTAN+ + IIGAL T++L   IG+LG+F +GA  + L  + T
Sbjct:  239 QETALDEGSPSKPVAMETSEHGNRGGELDSTPGQMVRKPQFWLLWATFLVNSQ---SVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA---------LMFVLSFGMAVLMSTFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLKDAIGWLGLFLLGAAFSTLGAVFT 448
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Match: EDO35238 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM28])

HSP 1 Score: 36.1946 bits (82), Expect = 1.496e-6
Identity = 18/61 (29.51%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  369 TIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQI 551
            ++  ++Y+M LTYG++FG+G  + Y  ++     +   +  L  G+  +G G+GS+A   I
Sbjct:  109 SVAPNIYIMDLTYGLMFGVGASMCYFPSVIILGTYFNKRLSLANGLTSAGSGVGSLAMGPI 169

HSP 2 Score: 33.4982 bits (75), Expect = 1.496e-6
Identity = 32/99 (32.32%), Postives = 47/99 (47.47%), Query Frame = 2 
Query:   11 LKEISENKLTNYK----GVLCVLGGFLIHF-SLGTIYTFGNMSPYIASYMKFICNFNVTNGDMLWIFAGGLTFQGITM--SLGGMLSKKIGTRLTVLIG 286
            L E  E  L +Y     G L  L  F+  F  LGT+  FG +      Y++ +  F V   +  W+  G +T+  + +   LG  L KKIG RLT ++G
Sbjct:    8 LDEKPE-PLVDYPDGGWGWLVCLAAFITQFIVLGTMNNFGVI------YVELLQEFKVGKAEAAWV--GSITYGMMFLLGPLGTTLCKKIGCRLTTVVG 97
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000062462.1 (SMESG000062462.1)

HSP 1 Score: 291.197 bits (744), Expect = 2.996e-95
Identity = 160/167 (95.81%), Postives = 164/167 (98.20%), Query Frame = 3 
Query:  714 TPISQTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNESL 1214
            T + +TFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTA+RLMTTMTTILMGTFYFCLLITNQIVAQIIHL+WICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTF AFILTFFHSNESL
Sbjct:  213 TSLYKTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAMRLMTTMTTILMGTFYFCLLITNQIVAQIIHLLWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFFAFILTFFHSNESL 379

HSP 2 Score: 264.233 bits (674), Expect = 8.934e-85
Identity = 128/129 (99.22%), Postives = 129/129 (100.00%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPISQ 728
            SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIET+RYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPISQ
Sbjct:   25 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETDRYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPISQ 153

HSP 3 Score: 49.2914 bits (116), Expect = 3.715e-6
Identity = 24/24 (100.00%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  227 MSLGGMLSKKIGTRLTVLIGSWTM 298
            MSLGGMLSKKIGTRLTVLIGSWTM
Sbjct:    1 MSLGGMLSKKIGTRLTVLIGSWTM 24
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 197.978 bits (502), Expect = 1.854e-67
Identity = 122/352 (34.66%), Postives = 195/352 (55.40%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPISQTF----------------GQLFIKS--DYYLS--------------------VVGSVGSIFNSFG------------------------RIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNES 1211
            S   +LTYF IK +L+ +I+TYG +FGIG G+ YSVA++  + W P  RGLV G VV+G+GLGS+ FN IQT FINP N+SPN T+  E+YF D  +LD +P CF+LIG +Y I+Q +G +LL  P  + +                 +L + +  DY +                     V+ ++ S++ SFG                        R++WG ++D +SY+  +  +T + +I+MGTFYF  +I + ++ QI++ +++C +   + G FA++PA  +  FG  N++  +G++FT  +++SI+GAL TS L+  I Y G F +      ++ ILT F   +S
Sbjct:   62 SLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPTKS 413

HSP 2 Score: 78.1814 bits (191), Expect = 1.854e-67
Identity = 40/61 (65.57%), Postives = 46/61 (75.41%), Query Frame = 2 
Query:  119 MSPYIASYMKFICNFN-VTNGDMLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSWTM 298
            MSPYIASY+K+  N   VTNGDM+WIF+  +  Q  TMSLGG+L KKIG RLT  IGSWTM
Sbjct:    1 MSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWTM 61
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000062468.1 (SMESG000062468.1)

HSP 1 Score: 188.348 bits (477), Expect = 8.598e-61
Identity = 89/140 (63.57%), Postives = 111/140 (79.29%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTP-ISQTFGQLFIKSD 758
            S SVMLTYFTIK ++Y+ I TYG LFG GIGIAYSVAI+ A+ W P +RGLVIGIVV GFGLGS+ FNQ+QT FINP+N SPNITI T++YF D+ ML+RVP CFLLIGG+Y  +Q +GC+L FTP I +  G++ +  +
Sbjct:   51 SISVMLTYFTIKENIYITIFTYGALFGAGIGIAYSVAIATAMKWYPARRGLVIGIVVGGFGLGSLVFNQVQTFFINPHNESPNITIGTDKYFGDKKMLERVPICFLLIGGIYASLQFIGCMLQFTPSIFEITGEIVVTEE 190

HSP 2 Score: 65.0846 bits (157), Expect = 8.598e-61
Identity = 27/47 (57.45%), Postives = 36/47 (76.60%), Query Frame = 2 
Query:  158 NFNVTNGDMLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSWTM 298
            N +++NGDM+W+FA    FQG+TM+ GG+L+KKIG R T LIG W M
Sbjct:    4 NQDISNGDMVWVFACAFAFQGLTMTFGGILNKKIGPRFTTLIGCWIM 50

HSP 3 Score: 194.126 bits (492), Expect = 2.414e-57
Identity = 96/167 (57.49%), Postives = 136/167 (81.44%), Query Frame = 3 
Query:  714 TPISQTFGQLFIKSDYYLSVVGSVGSIFNSFGRIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNESL 1214
            T + +TFGQLFI  D++L+ VGS+ S FN+ GRI WG+I+DKYSYK A+R +T +TT+ +GTF  C L+ ++  ++I+  +W+C++L NICG+F+I+PAA +K FG+KNV+TI+GMLF+AN+IASI GAL+TSIL+SK+GYLGIFE+GAGM  +AF+LTF H+   L
Sbjct:  251 TTLYKTFGQLFINDDFFLATVGSIASFFNASGRIFWGQIMDKYSYKVAMRCITIITTVFIGTFICCKLVMHENGSKIMLTVWVCTLLANICGVFSILPAACSKAFGVKNVATIYGMLFSANAIASIFGALSTSILLSKVGYLGIFEIGAGMAIIAFLLTFAHAKSDL 417
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 197.978 bits (502), Expect = 1.009e-59
Identity = 122/352 (34.66%), Postives = 195/352 (55.40%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPISQTF----------------GQLFIKS--DYYLS--------------------VVGSVGSIFNSFG------------------------RIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNES 1211
            S   +LTYF IK +L+ +I+TYG +FGIG G+ YSVA++  + W P  RGLV G VV+G+GLGS+ FN IQT FINP N+SPN T+  E+YF D  +LD +P CF+LIG +Y I+Q +G +LL  P  + +                 +L + +  DY +                     V+ ++ S++ SFG                        R++WG ++D +SY+  +  +T + +I+MGTFYF  +I + ++ QI++ +++C +   + G FA++PA  +  FG  N++  +G++FT  +++SI+GAL TS L+  I Y G F +      ++ ILT F   +S
Sbjct:   40 SLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPTKS 391

HSP 2 Score: 51.9878 bits (123), Expect = 1.009e-59
Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = 2 
Query:  182 MLWIFAGGLTFQGITMSLGGMLSKKIGTRLTVLIGSWTM 298
            M+WIF+  +  Q  TMSLGG+L KKIG RLT  IGSWTM
Sbjct:    1 MVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWTM 39
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 197.593 bits (501), Expect = 4.592e-59
Identity = 122/352 (34.66%), Postives = 195/352 (55.40%), Query Frame = 3 
Query:  342 SFSVMLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIGIVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKCFLLIGGMYGIMQLLGCLLLFTPISQTF----------------GQLFIKS--DYYLS--------------------VVGSVGSIFNSFG------------------------RIMWGEIVDKYSYKTAVRXXXXXXXXXXXXFYFCLLITNQIVAQIIHLMWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGALTTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNES 1211
            S   +LTYF IK +L+ +I+TYG +FGIG G+ YSVA++  + W P  RGLV G VV+G+GLGS+ FN IQT FINP N+SPN T+  E+YF D  +LD +P CF+LIG +Y I+Q +G +LL  P  + +                 +L + +  DY +                     V+ ++ S++ SFG                        R++WG ++D +SY+  +  +T + +I+MGTFYF  +I + ++ QI++ +++C +   + G FA++PA  +  FG  N++  +G++FT  +++SI+GAL TS L+  I Y G F +      ++ ILT F   +S
Sbjct:   25 SLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPTKS 376
The following BLAST results are available for this feature:
BLAST of MFS domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Y59A8B.216.900e-3241.94pep chromosome:WBcel235:V:17986260:17994546:1 gene... [more]
F10G7.51.092e-2725.57pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.51.399e-2725.57pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.51.399e-2725.57pep chromosome:WBcel235:II:4693542:4696408:1 gene:... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P37662|YHJX_ECOLI3.694e-721.63Uncharacterized MFS-type transporter YhjX OS=Esche... [more]
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BLAST of MFS domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YF061.162e-6863.36Slc16a-5 (Fragment) OS=Schmidtea mediterranea OX=7... [more]
A0A3S1CE333.235e-6335.81Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
K1QXL99.212e-6337.72Translationally-controlled tumor-like protein OS=C... [more]
A0A1I8H2051.222e-5654.26MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A1I8HL501.626e-5654.26MFS domain-containing protein OS=Macrostomum ligna... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO352381.496e-629.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062462.12.996e-9595.81SMESG000062462.1[more]
SMESG000060691.11.854e-6734.66SMESG000060691.1[more]
SMESG000062468.18.598e-6163.57SMESG000062468.1[more]
SMESG000060691.11.009e-5934.66SMESG000060691.1[more]
SMESG000060691.14.592e-5934.66SMESG000060691.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021377 ID=SMED30021377|Name=MFS domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1258bp
GTTTGAACTACTAAAAGAAATATCAGAAAATAAATTGACTAACTATAAAG
GAGTTCTCTGTGTACTTGGTGGATTCTTAATCCACTTTTCCTTAGGAACT
ATTTACACATTTGGGAACATGAGCCCTTATATTGCATCATACATGAAATT
TATTTGTAATTTTAATGTTACAAATGGTGACATGCTGTGGATATTTGCTG
GCGGGTTAACTTTCCAAGGAATTACAATGTCTTTAGGAGGGATGCTTAGT
AAAAAGATAGGAACCAGATTAACTGTTTTAATAGGGAGTTGGACTATGAG
GTGTTTTGAATAAACATTTGAATTAATTGACTGTTTCTAATAGTTTTTCT
GTAATGCTGACTTATTTTACTATTAAATCTCACTTGTATTTAATGATATT
AACGTATGGCGTTCTATTTGGTATAGGAATTGGTATTGCTTATTCGGTTG
CAATATCAGCCGCTATTAACTGGCTTCCCAATCAAAGAGGTTTAGTAATT
GGAATCGTCGTTTCAGGATTTGGGCTTGGATCTATGGCTTTCAATCAAAT
ACAAACTCTATTTATTAATCCAAACAACCTCTCACCAAATATTACAATCG
AAACTGAAAGATATTTTGATGATCGAGACATGCTGGATAGAGTTCCAAAA
TGCTTTTTACTAATTGGTGGAATGTATGGAATAATGCAATTACTTGGATG
CCTTTTATTGTTTACTCCAATTTCACAGACATTTGGCCAATTATTTATCA
AAAGTGATTATTATTTATCAGTTGTCGGGTCAGTAGGCTCTATTTTCAAT
TCATTCGGTAGAATAATGTGGGGCGAAATTGTAGACAAATACTCTTATAA
AACTGCAGTGCGACTTATGACTACAATGACCACTATTTTAATGGGGACGT
TTTATTTCTGTCTTTTAATTACTAATCAAATTGTTGCCCAAATTATACAT
CTAATGTGGATTTGCTCAATTTTAGTAAATATTTGCGGAATCTTTGCTAT
AATGCCGGCTGCCATAGCAAAGTTATTTGGCATAAAAAATGTATCTACCA
TTTTCGGAATGTTGTTTACGGCTAATTCTATTGCCAGCATCATTGGAGCA
CTAACCACGAGCATTCTGATTTCAAAAATTGGATATTTAGGCATTTTTGA
AATGGGAGCTGGAATGACATTTTTGGCTTTTATTCTGACGTTTTTTCATT
CTAATGAAAGCCTTTAGATGTTGTAAACAAATAAAATTAGAAATTAGATG
GCAGATCG
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protein sequence of SMED30021377-orf-1

>SMED30021377-orf-1 ID=SMED30021377-orf-1|Name=SMED30021377-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=288bp
MLTYFTIKSHLYLMILTYGVLFGIGIGIAYSVAISAAINWLPNQRGLVIG
IVVSGFGLGSMAFNQIQTLFINPNNLSPNITIETERYFDDRDMLDRVPKC
FLLIGGMYGIMQLLGCLLLFTPISQTFGQLFIKSDYYLSVVGSVGSIFNS
FGRIMWGEIVDKYSYKTAVRLMTTMTTILMGTFYFCLLITNQIVAQIIHL
MWICSILVNICGIFAIMPAAIAKLFGIKNVSTIFGMLFTANSIASIIGAL
TTSILISKIGYLGIFEMGAGMTFLAFILTFFHSNESL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR020846MFS_dom
IPR011701MFS
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 99..287
e-value: 2.2E-13
score: 51.7
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 1..74
e-value: 7.0E-7
score: 30.1
NoneNo IPR availablePANTHERPTHR11360:SF3OXALATE/FORMATE ANTIPORTERcoord: 3..123
coord: 121..281
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 3..123
coord: 121..281
NoneNo IPR availableCDDcd17353MFS_OFA_likecoord: 1..281
e-value: 2.84656E-36
score: 131.578
NoneNo IPR availableTMHMMTMhelixcoord: 259..281
NoneNo IPR availableTMHMMTMhelixcoord: 15..34
NoneNo IPR availableTMHMMTMhelixcoord: 171..193
NoneNo IPR availableTMHMMTMhelixcoord: 232..254
NoneNo IPR availableTMHMMTMhelixcoord: 47..69
NoneNo IPR availableTMHMMTMhelixcoord: 200..222
NoneNo IPR availableTMHMMTMhelixcoord: 136..156
NoneNo IPR availableTMHMMTMhelixcoord: 101..123
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 122..282
e-value: 6.8E-9
score: 35.1
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 9..282