Phosphorylated adapter RNA export protein

Overview
NamePhosphorylated adapter RNA export protein
Smed IDSMED30021318
Length (bp)1428
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Phosphorylated adapter RNA export protein (SMED30021318) t-SNE clustered cells

Violin plots show distribution of expression levels for Phosphorylated adapter RNA export protein (SMED30021318) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phosphorylated adapter RNA export protein (SMED30021318) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phosphorylated adapter RNA export protein (SMED30021318) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021318

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30021318SMESG000002740.1 SmedASXL_007939SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Human
Match: PHAX (phosphorylated adaptor for RNA export [Source:HGNC Symbol;Acc:HGNC:10241])

HSP 1 Score: 59.3066 bits (142), Expect = 6.887e-9
Identity = 33/97 (34.02%), Postives = 58/97 (59.79%), Query Frame = 3 
Query:  702 QDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDN 989
            + +++I  +  +E  A  I+  L+E   DLI R +  +G +K+   L  T  +E NGG+   +GSR RT GGVF  L+K+  +I E+++K+  +++N
Sbjct:  216 KGRYEITAEDSQEKVADEISFRLQEPKKDLIARVVRIIGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYIEN 312
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Celegans
Match: Y71H2B.2 (pep chromosome:WBcel235:III:2623170:2627771:1 gene:WBGene00022192.1 transcript:Y71H2B.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y71H2B.2)

HSP 1 Score: 70.4774 bits (171), Expect = 5.140e-13
Identity = 34/78 (43.59%), Postives = 49/78 (62.82%), Query Frame = 3 
Query:  753 RIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLD 986
            ++A+ + E++ D +++    +G E S      TK IE NGGM T DGSR RT GGVF TL K D ++ +E+K+ IF D
Sbjct:  203 QMAAAMGEKDPDTVKKITNAIGSELSIKLFNETKEIEQNGGMKTADGSRRRTPGGVFITLFKMDSSVSREIKDGIFGD 280
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Fly
Match: Phax (gene:FBgn0033380 transcript:FBtr0088569)

HSP 1 Score: 65.0846 bits (157), Expect = 3.479e-11
Identity = 43/125 (34.40%), Postives = 72/125 (57.60%), Query Frame = 3 
Query:  744 TARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDES----ESLW--WKSVEINQYQIRNKKKIPNPGRRKEF 1100
             A  +AS+L E+  +L+ R +  LG++      + T+RIE++GGM  K+G R RT GGVF  LIK   NI QE +++IF ++  ++ +S    E+L    K  E+ +   +   ++P   +RKE+
Sbjct:  147 VATEMASKLYEEKDELLVRVVEVLGIDVCLELYKETQRIEADGGMMIKNGIRRRTPGGVFLFLIKHHDNITQEQQKRIFSEDRQSLSKSRKQIETLMRDRKVEELKKCLSKQVTELPTLNQRKEY 271
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Fly
Match: Phax (gene:FBgn0033380 transcript:FBtr0088568)

HSP 1 Score: 65.4698 bits (158), Expect = 4.225e-11
Identity = 43/124 (34.68%), Postives = 72/124 (58.06%), Query Frame = 3 
Query:  747 ARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDES----ESLW--WKSVEINQYQIRNKKKIPNPGRRKEF 1100
            A  +AS+L E+  +L+ R +  LG++      + T+RIE++GGM  K+G R RT GGVF  LIK   NI QE +++IF ++  ++ +S    E+L    K  E+ +   +   ++P   +RKE+
Sbjct:  244 ATEMASKLYEEKDELLVRVVEVLGIDVCLELYKETQRIEADGGMMIKNGIRRRTPGGVFLFLIKHHDNITQEQQKRIFSEDRQSLSKSRKQIETLMRDRKVEELKKCLSKQVTELPTLNQRKEY 367
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Zebrafish
Match: phax (phosphorylated adaptor for RNA export [Source:ZFIN;Acc:ZDB-GENE-040822-42])

HSP 1 Score: 58.9214 bits (141), Expect = 4.887e-9
Identity = 33/79 (41.77%), Postives = 46/79 (58.23%), Query Frame = 3 
Query:  756 IASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNN 992
            IA  L E   DLIER +  +G +K+   L  T  IE +GG+ T DGSR RT GGV+  L+K+  +I     ++IF + N
Sbjct:  219 IAHRLMEPKKDLIERVVRVIGNKKAIELLSETATIEQSGGLYTVDGSRRRTPGGVYLNLLKNTPSISHGQVKEIFHEEN 297
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Xenopus
Match: ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:Xenbase;Acc:XB-GENE-1010736])

HSP 1 Score: 57.7658 bits (138), Expect = 1.799e-8
Identity = 34/85 (40.00%), Postives = 48/85 (56.47%), Query Frame = 3 
Query:  756 IASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDES 1010
            IA  L+E   DLIER +  LG +K+   L  T  +E NGG+   +GSR RT GGV+  L+K+  +I  +  ++IF   N    ES
Sbjct:  238 IAHRLREPKKDLIERVVKTLGTKKAIELLMETAEVEHNGGLFILNGSRRRTPGGVYLNLLKNTPSITSDQVKEIFCLENQKEQES 322
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Mouse
Match: Phax (phosphorylated adaptor for RNA export [Source:MGI Symbol;Acc:MGI:1891839])

HSP 1 Score: 60.077 bits (144), Expect = 2.086e-9
Identity = 35/97 (36.08%), Postives = 57/97 (58.76%), Query Frame = 3 
Query:  702 QDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDN 989
            + +++I  +   E  A  IA  L+E   DLI R +  LG +K+   L  T  +E NGG+   +GSR RT GGVF  L+K+  +I E+++K+  +++N
Sbjct:  185 KGRYEITEEDAPEKVADEIAFRLQEPKKDLIARVVRILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYVEN 281
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Mouse
Match: Phax (phosphorylated adaptor for RNA export [Source:MGI Symbol;Acc:MGI:1891839])

HSP 1 Score: 60.077 bits (144), Expect = 2.377e-9
Identity = 35/95 (36.84%), Postives = 56/95 (58.95%), Query Frame = 3 
Query:  708 QFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDN 989
            +++I  +   E  A  IA  L+E   DLI R +  LG +K+   L  T  +E NGG+   +GSR RT GGVF  L+K+  +I E+++K+  +++N
Sbjct:  209 RYEITEEDAPEKVADEIAFRLQEPKKDLIARVVRILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYVEN 303
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. UniProt/SwissProt
Match: sp|Q9JJT9|PHAX_MOUSE (Phosphorylated adapter RNA export protein OS=Mus musculus OX=10090 GN=Phax PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.663e-8
Identity = 35/95 (36.84%), Postives = 56/95 (58.95%), Query Frame = 3 
Query:  708 QFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDN 989
            +++I  +   E  A  IA  L+E   DLI R +  LG +K+   L  T  +E NGG+   +GSR RT GGVF  L+K+  +I E+++K+  +++N
Sbjct:  209 RYEITEEDAPEKVADEIAFRLQEPKKDLIARVVRILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYVEN 303
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. UniProt/SwissProt
Match: sp|Q63068|PHAX_RAT (Phosphorylated adapter RNA export protein OS=Rattus norvegicus OX=10116 GN=Phax PE=1 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 2.548e-8
Identity = 34/85 (40.00%), Postives = 51/85 (60.00%), Query Frame = 3 
Query:  738 ETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDN 989
            E  A  IA  L+E   DLI R +  LG +K+   L  T  +E NGG+   +GSR RT GGVF  L+K+  +I E+++K+  +++N
Sbjct:  219 EKVADEIAFRLQEPKKDLIARVVTILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYIEN 303
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. UniProt/SwissProt
Match: sp|Q9H814|PHAX_HUMAN (Phosphorylated adapter RNA export protein OS=Homo sapiens OX=9606 GN=PHAX PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 3.307e-8
Identity = 33/97 (34.02%), Postives = 58/97 (59.79%), Query Frame = 3 
Query:  702 QDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDN 989
            + +++I  +  +E  A  I+  L+E   DLI R +  +G +K+   L  T  +E NGG+   +GSR RT GGVF  L+K+  +I E+++K+  +++N
Sbjct:  216 KGRYEITAEDSQEKVADEISFRLQEPKKDLIARVVRIIGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYIEN 312
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. UniProt/SwissProt
Match: sp|Q3MHI4|PHAX_BOVIN (Phosphorylated adapter RNA export protein OS=Bos taurus OX=9913 GN=PHAX PE=2 SV=2)

HSP 1 Score: 58.151 bits (139), Expect = 6.665e-8
Identity = 34/112 (30.36%), Postives = 63/112 (56.25%), Query Frame = 3 
Query:  708 QFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDNNDTIDESESLWWKSVEI 1040
            +++I     +E  A  I+  L+E   DLI R +  +G +K+   L  T  +E NGG+   +GSR RT GGVF  L+K+  +I E+++K+  +L+N    +  ++   + +++
Sbjct:  218 RYEITEDDSQERVADEISFRLQEPKKDLIARVVRIIGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYLENQKEYENKKAARKRRIQV 329
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. UniProt/SwissProt
Match: sp|Q5ZLY0|PHAX_CHICK (Phosphorylated adapter RNA export protein OS=Gallus gallus OX=9031 GN=PHAX PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 1.121e-7
Identity = 34/95 (35.79%), Postives = 54/95 (56.84%), Query Frame = 3 
Query:  708 QFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNI-EQEVKEQIFLDN 989
            +++I  +   E  A  IA  L E   DLI R +  +G  K+   L  T  +E NGG+   +G+R RT GGV+  L+K+  +I E+++KE  +L+N
Sbjct:  223 RYEITEEDSEEKVADEIAYRLCEPKKDLIARVVKIIGKRKAIELLMETAEVEQNGGLFIVNGTRRRTPGGVYLNLLKNTPSIKEEQIKEIFYLEN 317
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. TrEMBL
Match: A0A131ZWX8 (RNA_GG_bind domain-containing protein OS=Sarcoptes scabiei OX=52283 GN=QR98_0017830 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.124e-11
Identity = 45/101 (44.55%), Postives = 58/101 (57.43%), Query Frame = 3 
Query:  690 THLNQDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNN 992
            + L+ + F+IEF+         IA +LKE  IDLI R +  LG EK +  +     IES GG+ T +  R R+ GGV F LIK+D NI  E KE IF DNN
Sbjct:  136 SKLSNETFRIEFE---------IAKKLKESRIDLIARAIKILGEEKIRRLVSEVNDIESVGGLMTTNYQRRRSPGGVLFHLIKNDPNISVEQKESIFGDNN 227
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. TrEMBL
Match: A0A437AVV2 (RNA_GG_bind domain-containing protein OS=Chilo suppressalis OX=168631 GN=evm_013088 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.280e-11
Identity = 36/81 (44.44%), Postives = 53/81 (65.43%), Query Frame = 3 
Query:  738 ETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIF 980
            E+ A  IA +L E+  DL+ R +  LG  K+     AT+++E++GGM   +G+R RTAGGVFF L+K D ++ QE+  QIF
Sbjct:  210 ESIANDIAEKLAEEKTDLVGRIVQLLGANKAMDLYRATQKVEADGGMLVMNGTRRRTAGGVFFFLLKRDDDVSQEMVTQIF 290
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. TrEMBL
Match: A0A146KNG3 (Phosphorylated adapter RNA export protein (Fragment) OS=Lygus hesperus OX=30085 GN=Phax_0 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.736e-11
Identity = 36/84 (42.86%), Postives = 53/84 (63.10%), Query Frame = 3 
Query:  741 TTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNN 992
            T A++IA +L+E N+DL+ R ++ LG   +  +   T+ IES GG+   +GSR RT+GGVF  LI+SD+ I      +IF D N
Sbjct:   55 TVAKQIAEKLQEPNLDLVMRIVLILGKTSAIEYFRKTREIESKGGLLIMNGSRRRTSGGVFIHLIRSDKQIPGSKLVEIFEDPN 138
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. TrEMBL
Match: A0A3M7R2S9 (Phosphorylated adapter RNA export OS=Brachionus plicatilis OX=10195 GN=BpHYR1_000104 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 5.429e-11
Identity = 40/94 (42.55%), Postives = 59/94 (62.77%), Query Frame = 3 
Query:  738 ETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDESESL 1019
            ++ A+ I+  L+E   D+I+RC+  LG +KS   L AT+ IES GG+PT DG R RT GGVFF L+K D  I    K +IF+ + +   + ++L
Sbjct:  107 DSVAKEISRILQEPKDDVIKRCVKILGKKKSLELLYATEDIESCGGIPTSDGYRRRTPGGVFFQLLKKDDTILSCQKSKIFIPDQERKKKQKAL 200
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. TrEMBL
Match: T1HVR5 (RNA_GG_bind domain-containing protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 6.296e-11
Identity = 37/81 (45.68%), Postives = 51/81 (62.96%), Query Frame = 3 
Query:  744 TARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLD 986
             A +I   L+E N DLI + +  LG+ K+    + T+ IE +GGM   DGSR RT GGVFF LI +D+NI + V ++IF D
Sbjct:   33 VAEQIRVNLQEPNGDLIVKIVSTLGVRKALLVYQKTRMIEESGGMLVHDGSRRRTPGGVFFHLITTDKNIPKTVIKEIFYD 113
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Cavefish
Match: phax (phosphorylated adaptor for RNA export [Source:NCBI gene;Acc:103033737])

HSP 1 Score: 63.929 bits (154), Expect = 1.290e-10
Identity = 36/95 (37.89%), Postives = 53/95 (55.79%), Query Frame = 3 
Query:  708 QFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNN 992
            +++I  +   E     IA  L E   +LIER +  +G +K+   L  T  IE NGG+ T DGSR RT GGV+  L+K+  +I  +  ++IF D N
Sbjct:  221 RYEITPEDPEEKVIDEIAHRLMEPKKELIERVVKVIGAKKAIELLSETATIEQNGGLYTVDGSRRRTPGGVYLNLLKNTPSITHDEVKEIFHDEN 315
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Sea Lamprey
Match: phax (phosphorylated adaptor for RNA export [Source:ZFIN;Acc:ZDB-GENE-040822-42])

HSP 1 Score: 60.8474 bits (146), Expect = 2.496e-10
Identity = 34/82 (41.46%), Postives = 49/82 (59.76%), Query Frame = 3 
Query:  747 ARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNN 992
            A  IA  L+E   DLI R +  +G++KS   L  T  IE NGG+   +G+R RT GGV+  L+KS  +I Q+  ++IF + N
Sbjct:  250 AEEIAYRLREAKEDLIVRVVNIIGVKKSLELLYETAEIERNGGLMIVNGTRRRTPGGVYLNLMKSTPSITQDQIKEIFHEEN 331
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Nematostella
Match: EDO33080 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST76])

HSP 1 Score: 56.225 bits (134), Expect = 8.105e-9
Identity = 31/80 (38.75%), Postives = 44/80 (55.00%), Query Frame = 3 
Query:  738 ETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQI 977
            E  A+ +A  L+E   DLI R +  +G  K+    E T  I+  GG+PT +  R RT+GGVF  L+K+    E   KEQ+
Sbjct:  223 EDIAKELADRLEEAKTDLIHRVVKCIGHTKALQLFEETLEIQEQGGIPTANKDRRRTSGGVFLYLLKAK---EYATKEQV 299
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Medaka
Match: phax (phosphorylated adaptor for RNA export [Source:NCBI gene;Acc:101155249])

HSP 1 Score: 48.9062 bits (115), Expect = 7.779e-6
Identity = 31/77 (40.26%), Postives = 46/77 (59.74%), Query Frame = 3 
Query:  756 IASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLD 986
            IA  L+E   DLIER +  +G +K+   L  T  +E +GG+ T DGSR RT GGV+  L+K+  +I +    +IF +
Sbjct:  234 IAYRLQEPKKDLIERVVKVVGKKKAIELLGETATLEESGGVYTMDGSRRRTPGGVYLNLLKNTPSISKSQIRKIFFE 310
[Close]
BLAST of Phosphorylated adapter RNA export protein vs. Planmine SMEST
Match: SMESG000002740.1 (SMESG000002740.1)

HSP 1 Score: 806.209 bits (2081), Expect = 0.000e+0
Identity = 431/435 (99.08%), Postives = 432/435 (99.31%), Query Frame = 3 
Query:   81 MIIKKKFNVPGLXXXXXXXXXXXXXLFLQESAKKIDNYETISINDSLSESDDDIIFVSFEKKRKHLNEVWRNIIECDLLDQRIHDIGLRKFRVNRDVESYDLPSYKLKRYKRTNLCRFRNNSYRRHNRTPRKEIIHISSDEDERPVCNYRQKKSMKTXXXXXXXXXXXXXXXXXXXSDACEVISDSDTDFDEGPKLSTWNKLITHLNQDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDESESLWWKSVEINQYQIRNKKKIPNPGRRKEFKSYTLIDLDYFECFKDFRYRYIPMMSNFPLCAKQITDEVELNVDEDAEDSVMEIDLNYDSNSLAESDEICPTGPPLEIICNNQHNDSDIFELGVN 1385
            MIIKKKFNVPGLEEISTDEDSDDDILFLQESAKKIDNYETISINDSLSESDDDIIFVSFEK RKHLNEVWRNIIECDLLDQRIHDIGLRKFRVNRDVESYDLPSYKLKRYKRTNLCRFRNNSYRRHNRTPRKEIIHISSDEDERPVCNYRQKK MKTDKIE+IVIEDDDENEEKDNSDACEVISDSDTDFDEGPKLSTWNKLITHLNQDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDESESLWWKSVEINQYQIRNKKKIPNPGRRKEFKSYTLIDLDYFECFKDFRYRYIPMMSNFPLCAKQITD VELNVDEDAEDSVMEIDLNYDSNSLAESDEICPTGPPLEIICNNQHNDSDIFELGVN
Sbjct:    1 MIIKKKFNVPGLEEISTDEDSDDDILFLQESAKKIDNYETISINDSLSESDDDIIFVSFEKNRKHLNEVWRNIIECDLLDQRIHDIGLRKFRVNRDVESYDLPSYKLKRYKRTNLCRFRNNSYRRHNRTPRKEIIHISSDEDERPVCNYRQKKGMKTDKIEEIVIEDDDENEEKDNSDACEVISDSDTDFDEGPKLSTWNKLITHLNQDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDESESLWWKSVEINQYQIRNKKKIPNPGRRKEFKSYTLIDLDYFECFKDFRYRYIPMMSNFPLCAKQITDGVELNVDEDAEDSVMEIDLNYDSNSLAESDEICPTGPPLEIICNNQHNDSDIFELGVN 435
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BLAST of Phosphorylated adapter RNA export protein vs. Planmine SMEST
Match: SMESG000070992.1 (SMESG000070992.1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.161e-13
Identity = 42/110 (38.18%), Postives = 59/110 (53.64%), Query Frame = 3 
Query:  750 RRIASELKEQNIDLIERCLIYLGLEKSQTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIFLDNNDTIDESESLWWKSVEINQYQIRNKKKIPN 1079
            + IA  L E  +DL+ +C   LGL+K   FL  T+ IE  GG+   DGSR R+ GGVF  LIK   +I  E K++IFL ++         + ++ + NQ   RN    PN
Sbjct:  172 KEIARILSEPKVDLLNKCYCILGLDKCLEFLYLTEDIEERGGLLVMDGSRRRSPGGVFLNLIKESADISSEDKKKIFLPHS---------FQRNSQHNQRPYRNPIAQPN 272
The following BLAST results are available for this feature:
BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PHAX6.887e-934.02phosphorylated adaptor for RNA export [Source:HGNC... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Y71H2B.25.140e-1343.59pep chromosome:WBcel235:III:2623170:2627771:1 gene... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Phax3.479e-1134.40gene:FBgn0033380 transcript:FBtr0088569[more]
Phax4.225e-1134.68gene:FBgn0033380 transcript:FBtr0088568[more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
phax4.887e-941.77phosphorylated adaptor for RNA export [Source:ZFIN... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ezh11.799e-840.00enhancer of zeste 1 polycomb repressive complex 2 ... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Phax2.086e-936.08phosphorylated adaptor for RNA export [Source:MGI ... [more]
Phax2.377e-936.84phosphorylated adaptor for RNA export [Source:MGI ... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9JJT9|PHAX_MOUSE1.663e-836.84Phosphorylated adapter RNA export protein OS=Mus m... [more]
sp|Q63068|PHAX_RAT2.548e-840.00Phosphorylated adapter RNA export protein OS=Rattu... [more]
sp|Q9H814|PHAX_HUMAN3.307e-834.02Phosphorylated adapter RNA export protein OS=Homo ... [more]
sp|Q3MHI4|PHAX_BOVIN6.665e-830.36Phosphorylated adapter RNA export protein OS=Bos t... [more]
sp|Q5ZLY0|PHAX_CHICK1.121e-735.79Phosphorylated adapter RNA export protein OS=Gallu... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A131ZWX82.124e-1144.55RNA_GG_bind domain-containing protein OS=Sarcoptes... [more]
A0A437AVV23.280e-1144.44RNA_GG_bind domain-containing protein OS=Chilo sup... [more]
A0A146KNG34.736e-1142.86Phosphorylated adapter RNA export protein (Fragmen... [more]
A0A3M7R2S95.429e-1142.55Phosphorylated adapter RNA export OS=Brachionus pl... [more]
T1HVR56.296e-1145.68RNA_GG_bind domain-containing protein OS=Rhodnius ... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
phax1.290e-1037.89phosphorylated adaptor for RNA export [Source:NCBI... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
phax2.496e-1041.46phosphorylated adaptor for RNA export [Source:ZFIN... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO330808.105e-938.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
phax7.779e-640.26phosphorylated adaptor for RNA export [Source:NCBI... [more]
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BLAST of Phosphorylated adapter RNA export protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000002740.10.000e+099.08SMESG000002740.1[more]
SMESG000070992.14.161e-1338.18SMESG000070992.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021318 ID=SMED30021318|Name=Phosphorylated adapter RNA export protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1428bp
TGCATTTAAATATTATTTTATTGTGTTAAATTTCATCTTTTATTTAATAT
AGTTTTATTTATATTGTTTGGATAGAACCAATGATTATTAAGAAAAAGTT
TAATGTTCCTGGTCTTGAAGAAATATCGACTGATGAGGATTCAGACGATG
ACATTTTATTTCTTCAAGAATCAGCCAAGAAAATTGACAATTATGAAACT
ATTTCAATTAATGATTCCTTATCCGAAAGTGACGATGATATAATTTTTGT
TAGCTTTGAAAAAAAACGAAAGCATTTAAATGAAGTTTGGCGTAACATTA
TTGAATGTGATTTATTAGACCAAAGAATTCACGATATTGGATTAAGGAAA
TTTCGAGTGAATCGTGACGTCGAAAGTTACGATTTACCGTCGTACAAGTT
AAAAAGATATAAAAGAACAAATCTTTGCCGATTTAGAAATAATAGTTATA
GGAGGCACAACAGAACACCGAGAAAAGAAATTATTCATATATCAAGTGAT
GAAGACGAGAGACCCGTTTGCAACTATCGGCAGAAAAAGAGCATGAAGAC
AGATAAAATTGAGAAAATTGTTATTGAGGATGATGATGAGAATGAAGAGA
AGGATAATTCTGATGCATGTGAAGTTATTTCCGATTCCGACACTGATTTT
GACGAGGGGCCAAAATTGAGCACATGGAATAAATTGATCACCCATCTGAA
TCAAGATCAGTTCCAAATAGAATTTCAGAGCGGCCGAGAAACAACTGCTC
GTAGGATTGCATCGGAATTGAAGGAACAAAATATTGACTTGATCGAGCGG
TGCTTGATTTATCTCGGCTTGGAAAAATCTCAGACGTTTTTGGAGGCCAC
TAAAAGAATCGAATCTAACGGCGGAATGCCAACAAAAGACGGCTCCAGGC
TGCGTACGGCTGGTGGTGTATTTTTCACTCTGATCAAGTCAGATAGAAAC
ATCGAACAGGAAGTGAAAGAGCAAATATTCCTCGATAACAACGATACAAT
TGATGAGAGTGAATCACTCTGGTGGAAATCCGTGGAAATCAATCAGTACC
AAATTAGAAATAAAAAGAAAATTCCCAATCCCGGGAGAAGAAAAGAGTTC
AAATCTTATACTCTCATTGATTTGGATTATTTCGAATGTTTCAAAGATTT
CAGATATCGATACATTCCGATGATGTCGAATTTCCCGTTATGCGCTAAGC
AAATTACTGATGAAGTAGAGTTGAATGTTGACGAGGACGCTGAAGATTCT
GTGATGGAAATCGATCTCAATTATGATTCGAATTCATTAGCGGAAAGTGA
TGAGATTTGCCCCACTGGGCCACCACTTGAAATCATTTGCAATAATCAGC
ACAATGATTCCGATATTTTTGAACTGGGCGTCAATTAGTGAGATGACTGC
CGTTTTAGATCTGTATATTTAATAACGC
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protein sequence of SMED30021318-orf-1

>SMED30021318-orf-1 ID=SMED30021318-orf-1|Name=SMED30021318-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=436bp
MIIKKKFNVPGLEEISTDEDSDDDILFLQESAKKIDNYETISINDSLSES
DDDIIFVSFEKKRKHLNEVWRNIIECDLLDQRIHDIGLRKFRVNRDVESY
DLPSYKLKRYKRTNLCRFRNNSYRRHNRTPRKEIIHISSDEDERPVCNYR
QKKSMKTDKIEKIVIEDDDENEEKDNSDACEVISDSDTDFDEGPKLSTWN
KLITHLNQDQFQIEFQSGRETTARRIASELKEQNIDLIERCLIYLGLEKS
QTFLEATKRIESNGGMPTKDGSRLRTAGGVFFTLIKSDRNIEQEVKEQIF
LDNNDTIDESESLWWKSVEINQYQIRNKKKIPNPGRRKEFKSYTLIDLDY
FECFKDFRYRYIPMMSNFPLCAKQITDEVELNVDEDAEDSVMEIDLNYDS
NSLAESDEICPTGPPLEIICNNQHNDSDIFELGVN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR038092PHAX_RNA-binding_sf
IPR039047PHAX
IPR019385PHAX_RNA-binding_domain
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005737cytoplasm
GO:0005813centrosome
GO:0015030Cajal body
Vocabulary: biological process
TermDefinition
GO:0006408snRNA export from nucleus
GO:0006810transport
GO:0015031protein transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038092Phosphorylated adapter RNA export protein, RNA-binding domain superfamilyGENE3DG3DSA:1.10.10.1440coord: 215..312
e-value: 1.5E-24
score: 87.9
IPR019385Phosphorylated adapter RNA export protein, RNA-binding domainPFAMPF10258RNA_GG_bindcoord: 223..303
e-value: 1.9E-25
score: 88.7
IPR039047Phosphorylated adapter RNA export proteinPANTHERPTHR13135CYTOSOLIC RESINIFERATOXIN BINDING PROTEIN RBP-26coord: 16..305