SMED30021292

Overview
NameSMED30021292
Smed IDSMED30021292
Length (bp)288
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021292

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
zeta neoblastSMED30021292SMESG000003824.1 dd_Smed_v4_12116_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30021292 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.015490:13284..13570 -1396
v31.001594:97008..97294 -1360
Homology
BLAST of SMED30021292 vs. Planmine SMEST
Match: SMESG000003824.1 (SMESG000003824.1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.748e-21
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3
Query:  158 INTYYQLAKGDDIISGVYFHGTPLAYTIPLRAISPLVQNVTLY 286
            INTYYQLA GDDIISGVYFHGTPLAY IPLRAISPL+QNVTLY
Sbjct:  309 INTYYQLANGDDIISGVYFHGTPLAYIIPLRAISPLLQNVTLY 351          
BLAST of SMED30021292 vs. Planmine SMEST
Match: SMESG000003824.1 (SMESG000003824.1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.883e-21
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3
Query:  158 INTYYQLAKGDDIISGVYFHGTPLAYTIPLRAISPLVQNVTLY 286
            INTYYQLA GDDIISGVYFHGTPLAY IPLRAISPL+QNVTLY
Sbjct:  307 INTYYQLANGDDIISGVYFHGTPLAYIIPLRAISPLLQNVTLY 349          
BLAST of SMED30021292 vs. Planmine SMEST
Match: SMESG000003824.1 (SMESG000003824.1)

HSP 1 Score: 84.7297 bits (208), Expect = 8.433e-21
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3
Query:  158 INTYYQLAKGDDIISGVYFHGTPLAYTIPLRAISPLVQNVTLY 286
            INTYYQLA GDDIISGVYFHGTPLAY IPLRAISPL+QNVTLY
Sbjct:  302 INTYYQLANGDDIISGVYFHGTPLAYIIPLRAISPLLQNVTLY 344          
BLAST of SMED30021292 vs. Planmine SMEST
Match: SMESG000003824.1 (SMESG000003824.1)

HSP 1 Score: 84.7297 bits (208), Expect = 8.463e-21
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3
Query:  158 INTYYQLAKGDDIISGVYFHGTPLAYTIPLRAISPLVQNVTLY 286
            INTYYQLA GDDIISGVYFHGTPLAY IPLRAISPL+QNVTLY
Sbjct:  304 INTYYQLANGDDIISGVYFHGTPLAYIIPLRAISPLLQNVTLY 346          
BLAST of SMED30021292 vs. Planmine SMEST
Match: SMESG000003824.1 (SMESG000003824.1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.076e-20
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3
Query:  158 INTYYQLAKGDDIISGVYFHGTPLAYTIPLRAISPLVQNVTLY 286
            INTYYQLA GDDIISGVYFHGTPLAY IPLRAISPL+QNVTLY
Sbjct:  304 INTYYQLANGDDIISGVYFHGTPLAYIIPLRAISPLLQNVTLY 346          
The following BLAST results are available for this feature:
BLAST of SMED30021292 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021292 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000003824.17.748e-2193.02SMESG000003824.1[more]
SMESG000003824.17.883e-2193.02SMESG000003824.1[more]
SMESG000003824.18.433e-2193.02SMESG000003824.1[more]
SMESG000003824.18.463e-2193.02SMESG000003824.1[more]
SMESG000003824.11.076e-2093.02SMESG000003824.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021292 ID=SMED30021292|Name=SMED30021292|organism=Schmidtea mediterranea sexual|type=transcript|length=288bp
CTTTATCAAGAATTATCAAAGTTATTATGTTAAATAAATGCAAGCCGAAA
ACTGGGATACATAGAAAATTAATTTTTGATCTTTTGTCATAATATAAATA
AAATACCAAAAAAATAATTAAAATAATTTTTATCTAGCCTTTTCGAACCT
TTTTTAGGTAAAGAGTAACATTCTGCACTAATGGAGAAATTGCCCTTAAT
GGTATTGTATAGGCTAAAGGTGTTCCATGGAAATAGACTCCGGAGATAAT
ATCATCTCCTTTTGCTAATTGATAGTAGGTGTTGATTG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast