Protein kinase domain-containing protein

Overview
NameProtein kinase domain-containing protein
Smed IDSMED30021288
Length (bp)3273
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein kinase domain-containing protein (SMED30021288) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein kinase domain-containing protein (SMED30021288) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein kinase domain-containing protein (SMED30021288) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein kinase domain-containing protein (SMED30021288) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021288

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30021288h1SMcG0020484 PL08004A2H01smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30021288h1SMcG0020484 dd_Smed_v4_4695_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021288h1SMcG0020484 dd_Smed_v4_4695_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021288h1SMcG0020484 dd_Smed_v4_4695_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021288h1SMcG0020484 Contig7676newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021288h1SMcG0020484 Contig7676uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021288h1SMcG0020484 Contig4131newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021288h1SMcG0020484 Contig4131uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymal cellSMED30021288h1SMcG0020484 dd_Smed_v6_4695_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|B2IZD3|BDLP_NOSP7 (Bacterial dynamin-like protein OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=Npun_R6513 PE=1 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.562e-8
Identity = 49/201 (24.38%), Postives = 108/201 (53.73%), Query Frame = 1
Query:  520 LLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDG-TKKRIEKEKFSKELT---AYIEKYDERDQVSCNSV---TINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDKS----EDIKDLK--KNVIKQI 1083
            LLV GD   GKS+F+N L+  ++LP  +   TA    L +G  +   +   DG + ++++ + F  + T   A  +K ++  + +   V    +    ++LQ G++I D+ GL +++  NE  L  ++N   ++F+++ + P    T+G    + ++I    +   +++F++N  DQ+ +S    +D+++L+  +N ++Q+
Sbjct:   72 LLVLGDMKRGKSTFLNALIGENLLPSDVNPCTAVLTVLRYGPEKKVTIHFNDGKSPQQLDFQNFKYKYTIDPAEAKKLEQEKKQAFPDVDYAVVEYPLTLLQKGIEIVDSPGLNDTEARNELSLGYVNNCHAILFVMRASQPC---TLGERRYLENYIKGRGL---TVFFLVNAWDQVRESLIDPDDVEELQASENRLRQV 266          
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|P40983|YOR6_CALSR (Uncharacterized protein in xynA 3'region (Fragment) OS=Caldicellulosiruptor sp. (strain Rt8B.4) OX=28238 PE=4 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 5.546e-6
Identity = 51/195 (26.15%), Postives = 90/195 (46.15%), Query Frame = 1
Query:  520 LLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGTKKRIEKEKFSKELTAY-IEKYDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNIT-GVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDKS--EDIKDLKKNVIKQIKER 1092
            L+V G    GKS+ IN ++  ++LP  +   T+   ++ +      +V+   G KK I  +    EL  Y  E+ + ++Q   +++ I      L   + I DT G+G     N  +  +  + +  V+F+L  + PI E     L KI +++         ++FVIN  D   K+  E+I     NVIK I ++
Sbjct:   50 LVVLGQFKRGKSTLINYMLGANLLPTGVLPLTSVITKIYYSPEVKVDVIFESGVKKEIPVD----ELDLYCTERGNPKNQKCVDTIEIGYPFDFLNKDVVIVDTPGIGSVYQHNTDVTYEFIDKSDAVVFVLSVDPPITEVEKQFLLKIAENV-------DKIFFVINKSDLTSKNEIEEIVSFTTNVIKDITKK 233          
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A1S3JCR2 (uncharacterized protein LOC106171979 OS=Lingula unguis OX=7574 GN=LOC106171979 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 3.280e-31
Identity = 197/967 (20.37%), Postives = 408/967 (42.19%), Query Frame = 1
Query:  337 EQVENIYKKIQRLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGTKK--RIE-KEKFSKELTAYIEKYDERDQ-VSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVIN----MCDQLDKSEDIKD---LKKNVIKQIKERCVGLNP-EVITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSVDCIVESEKFTEDDRFEKQDYIRKLKNSVKDHKKSKESFTEKSNDLIRKYYNNFSKIIKPHFNQQGTRRKVIEEVVQNDYMNFGMSXXXXXXXXXXXXTCNAIMKEFKEFAVKEKMLEKCNGEMLELFLKHYATHLNNKHEYNKIIAAHIDSTMNRIESDLKITNLNDL-------LALSNEKRKILSVFAPLIIPVVRMTCTIKQE-FGNL--FSFFLSTMNRSNIHIFVNNPKSYIRKKSNVYYKKFFPKHK--DTIINSFLHSTGEATKKFKDYLEESMNDLTTNIFLLEDDMRKRKMVIKTYKDIKSGLESIRENFCRFYLLHYHKDKYNVMIDQIDSDLKAESGSEPILINRNRVVYNGHFKDMDKTDSSILMYK---SLDKKTMKDLYLEYIKHKEIVQLTQKDSSLPSVNCFMKIRFHILSNVGDPNYVYPMFIINGIDCSLRKYIDDFRSEIPRQSDECLYNEICRILKQVLYGLEFLHEQTPPSNHYDLTIDSIGYNSTQNAIKLSSISNETKVLVDYKQTTTKYVEEMSHLPLKILEENSEVKYSKTTDIYCLGTILYYEWVGTLPLFKEILDKQINTIKKLVDYLHLHNIQSGVLSGCFKSNFGSKM--------LKACKMCWKDQTMIR 3132
            EQ+  +Y+ I+R     +K+ E       DPL  +++     F       +K    + KC +L  G++ +GKSS +NL++  DILP S  +NT+    +++     A++   D TK    IE  E F  EL   I   +ER +        I M   IL+ GL I D+ G+GE+      +L  L      I+++   N  G  T   L  ++  +     P     F  N    +C++ D+   IK+   +K + + +++    G N  ++    T +  +  +   +S ++  LL  I+ L   S+  K +  Y +L NFV + +  +          + ++      +++ +   KK  + + E+    I   + +    +K +  +   R ++ +            +  +   +E   +    +++++++   + K  +K   E+ E F   +            +IA H D+ ++          +N+        L+L  + + IL V AP+++PV      I     G L       + +    +  + N+P +++RK+S     +F  K K  +  + S L       ++ ++ +   +      I  L+DD R+    ++ Y  +     S ++    F  +   ++  +   D I+     E   + +     +VV       + +T S++ + K    +  +T+ D    ++  +E  Q    ++          ++F+   +  D + VY  FI+  + CSLR Y+ +  +    ++DE  Y     I++QV+ GLE+LH ++    HYDL++D++  +  ++ +KL++I +   + +D  +     +   SHLP ++L  +    Y+ T+D+Y +G +++  W G    F++ +       + + D++     +   L   F SN  S++        LK  ++CW  +   R
Sbjct:  192 EQLAALYEDIERTSASIIKMEE-------DPLPGQLETSIPGF-MAKLKKEKERLKNKKCPILFAGETSAGKSSLLNLILGDDILPTSHLSNTSVICEISYAEQRRAKIHYWDETKTPDTIEDGEDFYAELAKKIHTKEERSKKWPYKKAEIFMPLDILKSGLCIVDSPGIGENQEMTGLVLDYLVEAFAFIYVINSANAGGVQT-DRLQALLREVANRKSPDEQQLFQANAAIFVCNKWDQVP-IKERELVKGDTLTKLQTCWDGCNASQLFCMDTAEASYFYKAGYISRDFGRLLDGIEHLVPISLENKVLFSYGWLYNFVTRVLHHLQAFLNQAHKSKVQRMKTYTDIQDRLTKLKKEADQYFEELKAEIESRFESALVTLKQYLMKPEIRERITKWTEPQQPKGHKWADVDTAIRERVVERITHLLEKWED---EHKFFKKLRPELTEKFTSQFRGIERQLTGIENMIARH-DTRLSTTSHKDPGEPVNEEEGFFPYDLSLKLKHKIILGVAAPILVPVAVAGAIIALPILGGLKVRQMIDARLAEEKLKKYKNDPINFLRKQSEKVLDEFVKKSKALEKYVRSELSQAYVCLQQLQEAVPRQIKADEAQILSLKDDQREASDYLRFYDPLNEVFVSFKKRLLYFKTIAIRRELKDYEFDDIEIPKTPEIICDSLYCTIFKVVLRKPIGSL-QTGSTVCLRKCHVPISHQTIHD----HLGVEEAYQYQHHENL---------VKFY--GSTRDFDNVY--FILEPLQCSLRCYLQETPTVDWDKTDEEKYMVFLEIMRQVVNGLEYLHSRSMQWVHYDLSLDTVAMD-YKDTVKLTNIGHRRMLKIDPTEPRENIL-RYSHLPHEVL-ADPPTTYTLTSDMYSVGIMMWEMWTGKRA-FEDEITANPTKFQTMSDFIQFCEDRRPGLE-YFYSNGASEVSSRHAETWLKTLRICWDPEPSTR 1121          
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: K1Q742 (Mitosis inhibitor protein kinase wee1 OS=Crassostrea gigas OX=29159 GN=CGI_10012076 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 1.232e-18
Identity = 118/478 (24.69%), Postives = 229/478 (47.91%), Query Frame = 1
Query:  115 IQRFDKESLIIIGDKFEIEDYENIADLKFQILAKILTKNQVNQNPEFHTEEIK------TIK--ESVLNEDKARRDFLFDYIEQVENIYKKIQRLDKIKVKLSENRIG-EKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGTKKRIE-------KEKFSKELTAYIEKYDERDQVSCNS-VTINMESSILQGGLDIYDTIGLGE--------SDFFNEHLLKDLSNITGVIFLLKKNNPIG--ENTVGGLSKII--------DHIMTNDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGL-NPEVITFSTKQ---EQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSVD---CIVESEKFTEDDRFEKQDYIRKLKNSVKDHKKSKESFTEKSNDLIR 1422
            ++  D++ +  IGD  E+ D   + D+           + + Q    H E++K      TI+  + +  EDK +R  + + +++++   KK +  +   V++       E+    +  +KN   +F QR+  +        +C ++V G++ +GKSSF+NLL+  DILPV     T+   ++++G    A ++  DGT + IE       KE+   ++  Y    D R++ S  S V +++  S+L+ G+ + D+ G+GE        + F +EH      +I G I+++K +N  G  E+ +G L K+I        D   T   P  ++ FV N+ D + KS++   +    +++++    GL    VI FS K+   E  L R + ++++YK  L ++++L   + + +    Y+++   + +SV     IV+S   TE          + LK  +KD     +S  +KS D+++
Sbjct:   22 VEEADEDEVFDIGDDLEV-DLSGLTDM-----------DGIRQRIIMHIEKVKGNYKDQTIEKLDDIAREDKRKRQKIVELLKKIQGTLKKFEVKEAPDVRMKRGSAALEELLVTEGAMKNFEIDFNQRVSQV-----TDGECMIVVSGETSAGKSSFLNLLLGEDILPVHHNPCTSVITKISYGAKRQARIIHMDGTTEDIEGIHKGELKERLWNKI--YETDLDSREKKSPTSEVQLSLPISLLKSGIVLVDSPGIGENSAMDSVITHFVSEH------HIMGFIYVIKSDNAGGVDEDRLGNLLKVIIDKQKKKLDDATTLFDPKCAI-FVCNLWDNV-KSDEQTAVYDYAVRKLENYWPGLVKTSVIRFSAKRAKAELSLDR-DYITKDYKLFLDNLKELLTKATDMRVKSTYKWMETVLVRSVHHLKTIVKSVDDTE----------QHLKVKMKDVHDKLQSLRQKSKDVLQ 461          
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A2B4RY20 (CBL-interacting serine/threonine-protein kinase 13 OS=Stylophora pistillata OX=50429 GN=CIPK13 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 9.141e-18
Identity = 85/374 (22.73%), Postives = 176/374 (47.06%), Query Frame = 1
Query:  229 NQVNQNPEFHTEEIKTIKESVLNEDKARRDFLFDYIEQVENIYKKIQRLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSI--RTNTACQVRLTFGGNEYAEVVE------RDGTKKRI----------EKEKFSKELTAYIEKYDERDQVSC-NSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDHIMT------NDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPE--VITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFV 1269
            NQ +  P +   +  ++      ED  ++  L  +  Q E  +     LD I + L    +G+ ++    R+++  +  +QR            +  LLV G++G+GKSS +NL++  ++LP SI   T+T C++R    G E   V+         G+  +I           ++ + ++++ Y+    +R++ S    V ++    +L+ G+ I D+ G+GESD  +E + + L      I++L   N  G      L K++  ++        + P     FV N  DQ+ K E I ++K +V+K++K+   GL+PE  ++  ST      QR  +++E++  L+  ++ +   ++  +  + + +L++ +
Sbjct:   47 NQSSNVPNWAAGQACSVLSEAREEDTRKKTALLQFFSQAETCFG---HLDDIMLNLVSMNMGDVNE----RMRSHRERMKQR------------EYFLLVAGETGAGKSSLVNLILGEELLPYSILSTTSTICEMRY---GKERRVVIHFKDKDPETGSPTKIFTLAEPPEGATEQNYLQQISPYVHLKSDREKGSIYKKVELHWPHQLLKNGIVIIDSPGVGESDIMDEIVTEYLPRAFAFIYVLNSANAGGVQK-DRLEKLVTKVVGKQKEGQEEFPPKCALFVCNKWDQVPKGE-INEVKSHVVKKLKKCWPGLDPESQIVYMSTMMANTAQRHGIITEDFSSLMNGVKAMVSRTIEARLEINWRWLDDLL 396          
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: C3YQV3 (Protein kinase domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_81322 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 9.174e-18
Identity = 105/415 (25.30%), Postives = 208/415 (50.12%), Query Frame = 1
Query:  181 NIADLKFQILAKILTKNQVNQNPEFHTEEIKTIKESVLNEDKARRDFLFDYIEQVENIYKKIQRLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGT-KKRI--EKEKFSKELTAYIEKYDERDQVS-CNSVTINMESSILQGGLDIY--DTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENT--VGGLSKIIDHIMTN---DVPSSSMYFVINMCDQLDKSE--DIKDLKKNVIKQIKERCVGLNP-EVITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSV---DCIVESEKFTEDDRFEKQDYIRKLKNSVKDH 1374
             + D+K ++   +L K   ++ P    E I  + E + + D+ RR+ L + +++VE   K+   L  +K +L        +D L  ++     NF  + E  K+ +K S KC +LV G++ +GKSS INLL+  ++LP S  +NT     + +G     ++  RDGT + R+  E +     L  Y+E+  +RDQ +    V I +    L+   DIY  D+ G+GE +   + + + L+     I+++K +N  G  T  +  L K +  + T+   D+P+ S    I +C++ D+    + K +K++ I++++    G +  +V   ST + Q L+ Q  +S ++   L  ++ L   S+N +  + Y +L+ F+ +++      V+  + + D+R  K + ++   +++ D 
Sbjct:   52 GLNDIKHRLKLHLLEKAGKSRKP---GEVINMVTE-MRSRDEERREHLLELLQEVE---KRAMGLMMMKQEL--------NDQLADQLDRNVPNFMAKFELHKERMK-SRKCPILVAGETSAGKSSLINLLLDEEVLPTSHLSNTYTICEIRYGDVRTIKLHRRDGTCEDRVLQEDDSLVDLLKKYVEEKGDRDQPTDYPRVEILLPRDFLK---DIYIVDSPGIGEKEEITKQVKEYLTEAFAFIYVIKSDNAGGVQTDRLQALLKEVATMKTSGTEDIPAFSAKTAIFVCNKWDQVPIAERKLVKEDTIRKLQACWSGSSARQVFCMSTAEAQRLRDQGHVSADFSRFLDGLEKLIPESLNWRIEVSYRWLDYFLSRTIYHLRAYVQQGEQSRDERKAKFELVKSRLHALGDQ 447          
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A1S3JP99 (uncharacterized protein LOC106174708 OS=Lingula unguis OX=7574 GN=LOC106174708 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 2.361e-16
Identity = 216/1030 (20.97%), Postives = 423/1030 (41.07%), Query Frame = 1
Query:  340 QVENIYKKIQRLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQ-----------RLE-----------NIKKTIKV--SFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVV--ERDGTKKRIEKE-KFSKELTAYIEK-------YDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSN--ITGVIFLL--KKNNPIGENTVGG-LSKIIDHIMTNDVP---SSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKE---RCVGLNPEVITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSVDCI---VESEKFTEDDRFEKQDYIRKLKNSVKDHKKSKESFTEKSNDLIRKYYNNFSK---IIKPHFNQQGTRRKVIEEVVQ--NDYMNFGMSXXXXXXXXXXXXTCNAIMKEFKEFAVKEKMLEKCNGEMLELFLKHYATHLNNKHEYNKII--AAHIDSTMNR-IESDLKITNLND-------LLALSNEKRKILSVFAPLIIPVVRMTCTIKQEFGNLFSFFLSTMNRSNIHIFVNNPKSYIRKKSNVYYKKFFPK----------------HKDTIINSFLHSTGEATKKFKDYLEESMNDL--TTNIFL--LEDDMRKRKMVIKTYKDIKSGLESIRENFCRFYLLHYHKDKYNVMIDQIDSDLKAESGSEPILINRNRVVYNGHFKDM-------DKTDS---SILMYKSLD-KKTMKDLYLEYIKHKEIVQLTQKDSSLPSVNCFMKIRFHILSNVGDPNYVYPMFIINGIDCSLRKYID--DFRSEIPRQSDECLYNEICRILKQVLYGLEFLHEQTPPSNHY---DLTIDSIGYNSTQN-AIKLSSISNETKVLVDYKQTTTKYVEEMSHLPLKILEENSEVKYSKTTDIYCLGTILYYEWVGTLPLFKEILDKQINTIKKLVDYLHLHNIQSGVLSGCFKSNFGS-KMLKACKMCWKDQTM 3126
             VE  + ++ RL+K     +E R  E D   ++ +++ FK  E+           RLE           N++K I +  +  C +LV G++ SGKS+ +NLL+ + ILP +  + TA   R+ +G       V  + DG+KK  E   K   +L + +E          E +Q  C +V + +   +L+ G+ I D+ G+GES   ++ +L  ++N  +   I+++   K+  + E+ +   L ++ D   T   P        FV N  D +      K L +NV   + E   R +  + ++ T S K      +   M+ +Y+ L++ I+ L   S+  +T  ++ +  + V +++DC    + S   T D+          L+      KK  +   +KS ++++K   +  K    I    ++      V + V    N    +G+S    +E  I +  C  I+   + +  +EK  E    ++ + F + +    +       ++  ++  DS+ +R + + +++ +L D       ++  +  ++  L +F+P+I+P+           G L +        S   +FV+  K   ++K     K++  K                 K  +  +++ +  +  +     + ES+  L  +T  ++  +++D R ++ +I TY  +      +      F + H     YN      ++D++A+     I + +    Y G++ ++        K  S   S+  YK    +K +++  +E  K K+     QK   +P+          IL    DP    P  I+     SLR        R  +P Q   CL  E    L Q+   L +LH+     N +   DL++D++   + ++  +KL +IS    +      +T   +  M +LP  +L+      Y    +IY LG +++  W              I    K+   L +    +G+  G F   F S KM    + CW ++TM
Sbjct:   48 HVEAEHSQVPRLEKAADVFAELR--ENDTQRRNALQSIFKTIEESLPLLDLSILKRLEAENVVSESADTNVRKRITMLETGNCPILVAGETSSGKSTLLNLLLGKKILPEAHLSCTAAITRIKYGEEPRVFKVTHQPDGSKKSSEVPIKKPDDLCSALEPVLFPKGAAREDEQEKC-TVDVYLPFELLKSGVYIIDSPGIGESGVMDDVVLDFIANNDVFAFIYVIMTDKSGGVQEDRLQKLLRQLQDMKDTAGEPIFDPGCAIFVCNRWDLVPGYAREKAL-ENVRLAVSEGWNRPMDSSKQIFTLSAKMAWQHSQAGYMTHDYRRLVRGIKGLIPVSLQRRTEHVHRWGKHMVERTIDCAEQYLHSANLTRDE----------LEAQHNSQKKRLDRLEKKSEEILKKLDEHLYKECETITDSLSKYLGSDPVCDRVTSWTNAEAPYGIS-WNYLEGRIEDAICKRIIDAIRSWETEEKHFETLKKDLFKKFTREFQLIESELRMIENMLERSSETDSSESRDLAAYMELQDLMDNEVNKMSIVDFNLGEKIALGLFSPIILPI--------GALGMLLA--------SPAFLFVDVKKHVRKRKEKQILKQYDQKKCEFLRDRSSEALKIMAKRDVTKAYVDAQLQEARDHLQMMRESIPQLISSTRKYMQSIKEDQRSKEQLIDTYDPVVKKFTRVNRALEDFAITHLFSYDYN------ETDVEAD-----IRLVKGPTSYKGNWTEVFSAILKSPKGPSLKISVKRYKGATCEKQVREDIIEMRKMKQ-----QKRRFIPT----------ILGTYLDPTSELPSLILTPRLTSLRVLSSGKGSRKVLPGQLVHCLLKEPIDCLIQLANALAYLHK-----NGFVCLDLSMDTVMITAGRDKTVKLVNISKPVTMPPPDPNST---IGSMVYLPYSVLDGTK--PYDADANIYALGLMMWEVW-------------HIEHAYKIHRDLVVSVFCAGIRQGNFPLKFESGKMDPTLEACW-EKTM 996          
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO49816 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG14])

HSP 1 Score: 59.6918 bits (143), Expect = 4.938e-9
Identity = 39/140 (27.86%), Postives = 74/140 (52.86%), Query Frame = 1
Query:  517 SLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGT---KKRIEKEKFSKELTAY-IEKYDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITG-VIFLLKKNN 921
            +L+V G +  GKSS IN  +   ++P   +  TA  VR+T+    Y  +V+RDGT   ++ ++  K  +E     I+  D+  +V+  +V   +    L+ G+D+ D+ G  E+D  +E +   + +I   VI+++  +N
Sbjct:  103 ALIVTGQTNCGKSSIINEFLGARVVPTKAQPCTARIVRMTYSETPYVRLVKRDGTELERRELKSNKIPREFIELNIDDRDDEKRVNA-TVEAGLCVDFLKAGIDVIDSPGTSENDVLDELVRNQMKSILPFVIYVVDGHN 241          
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO49580 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGQ2])

HSP 1 Score: 53.9138 bits (128), Expect = 3.383e-7
Identity = 43/150 (28.67%), Postives = 77/150 (51.33%), Query Frame = 1
Query:  502 VSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDG---------TKKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSILQGG-LDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNN 921
            VS K ++LV G + SGKSS IN L+    LP +    T+  VR+ +  N Y EV   +G          KKRI +++ + +     +K  + + V C  V + + +S+L+GG L++ D  G+ E++  ++ + + +  I  V+  +   N
Sbjct:   89 VSKKAAILVVGQTNSGKSSLINELLGGTYLPTAEIPCTSRIVRICYSDNNYVEVQGPNGECVQGKQSFGKKRIPRDEIALDDD---KKRSDAEWVRC-VVKVGLNNSLLRGGHLELVDAPGMSENEVLDQIVQECIHGILQVVIYVIDGN 234          
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000010251.1 (SMESG000010251.1)

HSP 1 Score: 2006.88 bits (5198), Expect = 0.000e+0
Identity = 1001/1022 (97.95%), Postives = 1008/1022 (98.63%), Query Frame = 1
Query:   70 MNPEEIDMEKLTESCIQRFDKESLIIIGDKFEIEDYENIADLKFQILAKILTKNQVNQNPEFHTEEIKTIKESVLNEDKARRDFLFDYIEQVENIYKKIQRLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGTKKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSVDCIVESEKFTEDDRFEKQDYIRKLKNSVKDHKKSKESFTEKSNDLIRKYYNNFSKIIKPHFNQQGTRRKVIEEVVQNDYMNFGMSXXXXXXXXXXXXTCNAIMKEFKEFAVKEKMLEKCNGEMLELFLKHYATHLNNKHEYNKIIAAHIDSTMNRIESDLKITNLNDLLALSNEKRKILSVFAPLIIPVVRMTCTIKQEFGNLFSFFLSTMNRSNIHIFVNNPKSYIRKKSNVYYKKFFPKHKDTIINSFLHSTGEATKKFKDYLEESMNDLTTNIFLLEDDMRKRKMVIKTYKDIKSGLESIRENFCRFYLLHYHKDKYNVMIDQIDSDLKAESGSEPILINRNRVVYNGHFKDMDKTDSSILMYKSLDKKTMKDLYLEYIKHKEIVQLTQKDSSLPSVNCFMKIRFHILSNVGDPNYVYPMFIINGIDCSLRKYIDDFRSEIPRQSDECLYNEICRILKQVLYGLEFLHEQTPPSNHYDLTIDSIGYNSTQNAIKLSSISNETKVLVDYKQTTTKYVEEMSHLPLKILEENSEVKYSKTTDIYCLGTILYYEWVGTLPLFKEILDKQINTIKKLVDYLHLHNIQSGVLSGCFKSNFGSKMLKACKMCWKDQTMIRD 3135
            MNPEEIDMEKLTESCIQRFD+ESLIIIGDKFEIEDYENIADLKFQILAKILTKNQVNQNPEFHTEEIKTIKESVLNEDKARRDFLFDYIEQVENIYKKIQ LDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSI+TNTACQVRLTFG NEYAEVVERDGTKKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSVDCIVESEKFTEDDRFEKQ+YIRKL+NSVKDHKKSKESFT+KSNDLIRKYYNNFSKIIK HFNQQGTRRKVIEEVVQ DYMNFGMSKEEEIEKEINE TCNAIMKEFKEFAVKEKMLEKC+GEMLELFLKHYA HLNNKHEYNKIIA HIDSTMNRIESDLKITNLNDLLALSNEKRKILSVFAPLIIPVVRMTCTIKQEFGNLFSFFLSTMNRSNIH+FVNNPKSYIRKKSNVYYKKFFPKH DTIINSFLHSTGEAT KFKDYLEESMNDLTTNIFLLEDDMRKRKMVIKTYKDIK GLESIRENFCRFYLLHYHKDKYNVMIDQIDSDLKA    EPILINRNRVVYNGHFKDMDKTDSSILMYKSLDKKTMKDLYLEYIKHKEIVQLTQKDSSLPSVNCFMKIRFHILSNVGDPNYVYPMFIINGIDCSLRKYIDDFRSEIPRQSDECLYNEICRILKQVLYGLEFLHEQTPPSNHYDLTIDSIGYNSTQNAIKLSSISNETKVLVDYKQTTTKYVEEMSHLPLKILEENSEVKYSKTTDIYCLGTILYYEWVGTLPLFKEILDKQINTIKKLVDYLHLHNIQSGVLSGCFKSNFGSKMLKACKMCWKDQTMIRD
Sbjct:    1 MNPEEIDMEKLTESCIQRFDRESLIIIGDKFEIEDYENIADLKFQILAKILTKNQVNQNPEFHTEEIKTIKESVLNEDKARRDFLFDYIEQVENIYKKIQSLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIKTNTACQVRLTFGENEYAEVVERDGTKKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSVDCIVESEKFTEDDRFEKQNYIRKLRNSVKDHKKSKESFTQKSNDLIRKYYNNFSKIIKAHFNQQGTRRKVIEEVVQKDYMNFGMSKEEEIEKEINEITCNAIMKEFKEFAVKEKMLEKCSGEMLELFLKHYAAHLNNKHEYNKIIATHIDSTMNRIESDLKITNLNDLLALSNEKRKILSVFAPLIIPVVRMTCTIKQEFGNLFSFFLSTMNRSNIHVFVNNPKSYIRKKSNVYYKKFFPKHIDTIINSFLHSTGEATNKFKDYLEESMNDLTTNIFLLEDDMRKRKMVIKTYKDIKRGLESIRENFCRFYLLHYHKDKYNVMIDQIDSDLKA----EPILINRNRVVYNGHFKDMDKTDSSILMYKSLDKKTMKDLYLEYIKHKEIVQLTQKDSSLPSVNCFMKIRFHILSNVGDPNYVYPMFIINGIDCSLRKYIDDFRSEIPRQSDECLYNEICRILKQVLYGLEFLHEQTPPSNHYDLTIDSIGYNSTQNAIKLSSISNETKVLVDYKQTTTKYVEEMSHLPLKILEENSEVKYSKTTDIYCLGTILYYEWVGTLPLFKEILDKQINTIKKLVDYLHLHNIQSGVLSGCFKSNFGSKMLKACKMCWKDQTMIRD 1018          
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000010252.1 (SMESG000010252.1)

HSP 1 Score: 788.489 bits (2035), Expect = 0.000e+0
Identity = 419/1021 (41.04%), Postives = 658/1021 (64.45%), Query Frame = 1
Query:   70 MNPEEIDMEKLTESCIQRFDKESLIIIGDKFEIEDYENIADLKFQILAKILTKNQVNQNPEFHTEEIKTIKESVLNEDKARRDFLFDYIEQVENIYKKIQRLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGTKKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVITFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFVWKSVDCIVESEKFTEDDRFEKQDYIRKLKNSVKDHKKSKESFTEKSNDLIRKYYNNFSKIIKPHFNQQGTRRKVIEEVVQNDYMNFGMSXXXXXXXXXXXXTCNAIMKEFKEFAVKEKMLEKCNGEMLELFLKHYATHLNNKHEYNKIIAAHIDSTMNRIESDLKITNLNDLLALSNEKRKILSVFAPLIIPVVRMTCTIKQEFGNLFSFFLSTMNRSNIHIFVNNPKSYIRKKSNVYYKKFFPKHKDT--IINSFLHSTGEATKKFKDYLEESMNDLTTNIFLLEDDMRKRKMVIKTYKDIKSGLESIRENFCRFYLLHYHKDKYNVMIDQIDSDLKAESGSEPILINRNRVVYNGHFKDMDKTDSSILMYKSLDKKTMKDLYLEYIKHKEIVQLTQKDSSLPSVNCFMKIRFHILSNVGDPNYVYPMFIINGIDCSLRKYIDDFRSEIPRQSDE--CLYNEICRILKQVLYGLEFLHEQTPPSNHYDLTIDSIGYNSTQNAIKLSSISNETKVLVDYKQTTTKYVEEMSHLPLKILEENSEVKYSKTTDIYCLGTILYYEWVGTLPLFKEILDKQINTIKKLVDYLHLHNIQSGVLSGCFKSNFGSKMLKACKMCWKDQ 3120
            M+P   D  ++  + I     + L  +G K +I  Y+++ +L+ +I   I+   +      ++TE+ K  KE+   ED+ RRD L  Y++++ +IY +IQ+LDK+K+    + + E+DDPL+++I+NEF N    L+  K  ++    C+LL+CGDSGSGKSSFINLLV   +LPVSIR+++ CQVRLT+G  E AEVV+R+ T  +I K +F  +L +Y+EK D+R ++SCNS TI + S IL+GGLDIYDTIGLGES+ FN+ +LKDL++ITGV+FLLKKNNPIG+NTVGGLSK+I HI TN +P  SM+F+INMCD L+K  + ++LK+NV KQ++++C  LNP++ TFS KQE F+Q  N MS+ Y++++  IQ L+INS+NT T+ ++EFL  FV K++D I+ESE FT DD+  K++YI+KL+N  +D K     F + +   ++  Y  + KII+ H   + T+RKV+ EV       FG++ ++EI++EI + T N+IMK FKEF  KE +L+ C  EML++FL+ +A H   K + N+ ++ HI++ M     D K  +LNDL+ L   +RK+L +F P+++P + +   +KQ F + +S  LS  N SN+H F  +  +Y+  +S+ YY KF  K ++   I++    +  + T+ F+ YL + MN L  NI LL++DMR ++ VIKTYK+IK  LE I    C+  LL   K+K +V++D+  S+L   +    +L NRN  +Y G FK+ +  +S + +YKS+D K     Y + I ++EI+      ++   +  F+    HILS+V D N++ PMF + GID +L+ ++  FR E+ +   E   LYNEI R+L+Q++ GL +LH   PP NHYDLT++SIG+N++   IKLSS+S +T+V ++Y   + + + +++HLP++ILE+ S+  Y++ +D+YCLGT+LY  W G  P    I   +I +++  V+ +        +    F + F   +   C +CW+++
Sbjct:    1 MDPINDDCNEIELASIDTKTDQELFELGKKLKITGYKSMVELRQKISLTIMMNKKSVIESSYYTEDFKMKKETTFKEDELRRDILKSYVDKIGSIYNEIQKLDKVKMTTGID-VSERDDPLQTKIRNEFNNITTELQRKKDLLEKIPNCALLICGDSGSGKSSFINLLVNNRVLPVSIRSSSVCQVRLTYGAVECAEVVDRNNTTIKIGKNEFGTKLKSYLEKLDDRTEISCNSATIYLNSPILEGGLDIYDTIGLGESEMFNDQVLKDLNDITGVLFLLKKNNPIGDNTVGGLSKVICHIATNGIPGGSMHFIINMCDLLEKFSEREELKENVEKQLRDKCSCLNPKITTFSAKQELFMQEHNYMSKEYENIMTLIQHLHINSINTNTVSVFEFLFRFVCKTIDYIIESENFTVDDKVTKKEYIKKLRNLEEDRKLLNLRFKKDNERFLKDKYEKYKKIIEKHLMSEDTKRKVVAEVNSKQSTLFGLTDKDEIDEEILKITNNSIMKSFKEFVEKEYILKSCCDEMLKIFLQQFAEHQKKKDDINQAMSRHINTVMETFTKDFKKMDLNDLIGLPVYQRKLLQLFTPIMVPGLEIKNIVKQRFESWYSAILSKFNLSNVHKFYEDTNTYVLNRSSQYYWKFVGKKRNIRGIVDYLFFAPLQVTQDFEKYLADDMNYLAKNIDLLDEDMRNKENVIKTYKEIKERLEIINNELCKIELLFMQKEKNDVVLDK--SELLKNTAE--LLFNRNFRIYTGSFKNKELANSKLFVYKSMDNKNWTKQYQDIINYREILLGDHDKNNSDYLKHFLTFDLHILSSVKDKNHLCPMFSVQGIDSTLKDHMGSFREEVSKLEVENNSLYNEIVRLLEQIINGLIYLHSHNPPLNHYDLTLESIGFNTSTAEIKLSSVSVKTQVEINYSFESKEELLQVTHLPIEILEKGSKELYTEASDMYCLGTVLYRLWTGIDPFANVIESSRIESVEMFVETMKKTKHHLIIDHSMFLNGFPDMIFAWCTLCWENK 1016          
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000056718.1 (SMESG000056718.1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.144e-8
Identity = 52/235 (22.13%), Postives = 114/235 (48.51%), Query Frame = 1
Query:  502 VSFKCSLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGT--KKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSI----LQGGLDIYDTIGLGESDFFNEHLLKDLSNITGV-IFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVITFSTKQEQFLQRQNLMSENYKH 1185
            VS   ++ + G   + KS+ IN+L+++ ++  +    TA   RLT+    Y E+ + DGT  K+++ +   + ++ A   + +E D+ +   +   +++ I    L  G+D+ DT GL E    +    + + NI  + I+++  N  + +  +  L +     + N +P   + + I+  D    +E + D      ++I++  + L+ E ++ + K+   LQ QN + + Y H
Sbjct:   91 VSCNPAIAIVGQVKAAKSTMINVLLRKTVVCQAFSRCTARITRLTYSEENYVELFDSDGTLLKRKLLR---APKIPARFVRLNENDRSNIKEIRKEVKAGINCKFLMSGIDLIDTPGLNEHSLLSNLSKETVKNILPIMIYVIDGNTSVKQTDLSDLLE-----LKNAIPDLKIIYFISKLDSDISTEQLSD-----PEEIEKMKIELSIEKLSIAFKR---LQNQNFIPKTYNH 309          
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000056716.1 (SMESG000056716.1)

HSP 1 Score: 57.7658 bits (138), Expect = 5.176e-8
Identity = 58/234 (24.79%), Postives = 121/234 (51.71%), Query Frame = 1
Query:  517 SLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGTKKRIEKEKF-SKELTAYIEKYDERDQVSCNSVT----INMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKI----IDHIMT--NDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVITFSTKQEQFLQRQNLMSENYKH 1185
            ++ + G   +GKS+ +NLL++++++  + +T TA   R+T+    Y E+V  +   + IE++K  +K +  YI + ++ D+ + + V     + ++S  L+ G+D+ D+ GL E+   N  LL  +SNI   +       P+    + G + +    I +I++    +P+ ++ + I   D  D SE I++   + IK  K++        + F+ ++   LQR NL+ + + H
Sbjct:  105 TIAIVGSVKAGKSTLVNLLLRKNVVRQAHKTCTARLTRITYSEENYVELV--NIHDQIIERKKLKNKPIPKYIVELNDEDRSNPDEVNKLIRVGIKSKFLKSGIDLIDSPGLNENHVLN--LL--ISNIINKVL------PVMIYVIDGQTSVKQTDISNILSLKVSIPNLNIIYFITRLDTDDVSELIEN---DHIKTAKDK--------LDFTFRK---LQRSNLIPKTFNH 312          
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000056716.1 (SMESG000056716.1)

HSP 1 Score: 57.7658 bits (138), Expect = 5.263e-8
Identity = 58/234 (24.79%), Postives = 121/234 (51.71%), Query Frame = 1
Query:  517 SLLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVERDGTKKRIEKEKF-SKELTAYIEKYDERDQVSCNSVT----INMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKI----IDHIMT--NDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVITFSTKQEQFLQRQNLMSENYKH 1185
            ++ + G   +GKS+ +NLL++++++  + +T TA   R+T+    Y E+V  +   + IE++K  +K +  YI + ++ D+ + + V     + ++S  L+ G+D+ D+ GL E+   N  LL  +SNI   +       P+    + G + +    I +I++    +P+ ++ + I   D  D SE I++   + IK  K++        + F+ ++   LQR NL+ + + H
Sbjct:  104 TIAIVGSVKAGKSTLVNLLLRKNVVRQAHKTCTARLTRITYSEENYVELV--NIHDQIIERKKLKNKPIPKYIVELNDEDRSNPDEVNKLIRVGIKSKFLKSGIDLIDSPGLNENHVLN--LL--ISNIINKVL------PVMIYVIDGQTSVKQTDISNILSLKVSIPNLNIIYFITRLDTDDVSELIEN---DHIKTAKDK--------LDFTFRK---LQRSNLIPKTFNH 311          
The following BLAST results are available for this feature:
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|B2IZD3|BDLP_NOSP73.562e-824.38Bacterial dynamin-like protein OS=Nostoc punctifor... [more]
sp|P40983|YOR6_CALSR5.546e-626.15Uncharacterized protein in xynA 3'region (Fragment... [more]
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BLAST of Protein kinase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3JCR23.280e-3120.37uncharacterized protein LOC106171979 OS=Lingula un... [more]
K1Q7421.232e-1824.69Mitosis inhibitor protein kinase wee1 OS=Crassostr... [more]
A0A2B4RY209.141e-1822.73CBL-interacting serine/threonine-protein kinase 13... [more]
C3YQV39.174e-1825.30Protein kinase domain-containing protein OS=Branch... [more]
A0A1S3JP992.361e-1620.97uncharacterized protein LOC106174708 OS=Lingula un... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO498164.938e-927.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495803.383e-728.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000010251.10.000e+097.95SMESG000010251.1[more]
SMESG000010252.10.000e+041.04SMESG000010252.1[more]
SMESG000056718.12.144e-822.13SMESG000056718.1[more]
SMESG000056716.15.176e-824.79SMESG000056716.1[more]
SMESG000056716.15.263e-824.79SMESG000056716.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021288 ID=SMED30021288|Name=Protein kinase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3273bp
CCTAAATGATAATCAATTTATTTTCACTTTGTTTATAGCAATAGATTTTA
AGTATCGAAGGCTCTTTCAATGAATCCAGAAGAAATAGACATGGAAAAAC
TAACAGAATCGTGTATTCAAAGATTCGACAAAGAATCCTTAATCATCATC
GGCGACAAGTTTGAAATCGAAGACTATGAAAATATTGCCGATTTAAAGTT
TCAAATTCTCGCGAAAATCCTCACCAAAAATCAAGTCAACCAAAATCCAG
AATTCCATACAGAGGAAATTAAAACAATAAAAGAAAGTGTATTAAATGAA
GATAAAGCGAGAAGAGATTTCCTATTCGATTACATTGAACAGGTAGAAAA
CATTTATAAGAAAATTCAGAGATTAGACAAAATAAAAGTCAAATTAAGTG
AAAATAGAATTGGAGAGAAAGATGATCCCTTGAAGTCCAGAATTAAAAAT
GAATTTAAAAATTTCGAACAACGATTGGAAAATATTAAAAAAACTATCAA
AGTATCCTTCAAATGCAGTTTACTTGTATGCGGTGATTCTGGTTCCGGAA
AAAGTTCTTTCATAAACCTTTTAGTTAAAAGGGACATTTTGCCAGTTTCC
ATTAGAACTAATACAGCCTGCCAAGTGAGACTGACATTTGGGGGAAATGA
ATATGCCGAGGTAGTCGAACGTGATGGCACAAAGAAAAGGATTGAAAAAG
AGAAATTTAGTAAAGAACTTACGGCATATATCGAGAAATATGACGAGAGA
GATCAAGTTTCTTGTAATTCGGTGACAATCAATATGGAAAGTTCAATCTT
ACAGGGAGGGCTAGACATTTATGACACGATTGGATTGGGAGAGTCGGATT
TCTTCAATGAGCATTTACTAAAGGACCTTAGCAACATTACTGGTGTTATA
TTTTTGTTAAAGAAAAACAATCCTATCGGAGAAAATACTGTAGGAGGGCT
GTCGAAAATCATAGACCATATCATGACAAATGATGTTCCTAGTAGTTCAA
TGTATTTCGTTATAAATATGTGCGATCAGTTAGACAAATCAGAAGATATT
AAAGATTTGAAAAAAAATGTCATAAAACAAATCAAGGAAAGATGCGTCGG
TCTGAACCCTGAAGTGATAACTTTCAGTACAAAGCAAGAACAGTTTTTAC
AAAGACAAAACTTAATGAGTGAAAATTATAAGCATTTATTGAAGCATATT
CAGGACTTATATATTAATTCTGTAAATACGAAGACTATCTTAATTTATGA
ATTTTTGAATAACTTTGTCTGGAAATCTGTCGACTGTATAGTAGAATCCG
AAAAGTTTACTGAAGATGATAGATTCGAAAAACAGGATTACATAAGAAAA
TTGAAAAATTCAGTAAAAGACCACAAGAAGTCAAAGGAATCATTCACAGA
GAAATCAAATGATCTTATAAGAAAATATTATAATAATTTCTCAAAAATTA
TAAAACCACATTTCAATCAACAAGGAACGAGAAGAAAAGTTATCGAAGAA
GTAGTCCAAAACGACTATATGAATTTTGGAATGTCGAAGGAAGAGGAAAT
AGAAAAGGAAATTAATGAGAAAACTTGCAATGCGATTATGAAGGAATTCA
AAGAATTTGCAGTGAAGGAGAAGATGCTAGAAAAATGCAATGGGGAAATG
CTGGAACTGTTTCTCAAACATTATGCCACCCATCTGAATAATAAACACGA
ATACAACAAGATTATCGCCGCCCACATTGATTCTACAATGAATCGCATTG
AGAGCGATTTAAAGATTACAAATCTAAACGATTTACTCGCTCTTTCAAAT
GAGAAAAGAAAGATTCTTTCAGTCTTTGCTCCGTTGATTATTCCGGTAGT
CCGAATGACCTGCACAATCAAACAGGAGTTTGGAAACTTGTTCTCATTCT
TTCTATCCACAATGAACAGATCCAACATCCATATATTCGTCAACAATCCA
AAGTCATATATTAGAAAGAAATCCAATGTTTATTACAAGAAATTCTTTCC
AAAACACAAAGACACAATCATAAACAGTTTCCTTCATTCGACCGGAGAAG
CTACAAAAAAATTCAAAGATTATCTGGAAGAAAGCATGAATGATCTTACG
ACAAACATTTTTCTACTCGAAGATGATATGAGAAAACGAAAAATGGTTAT
TAAGACGTATAAAGACATTAAAAGTGGTCTGGAATCTATTAGAGAAAATT
TCTGTAGATTTTATCTGCTTCATTATCACAAAGACAAGTACAATGTAATG
ATTGATCAAATAGACTCAGATTTGAAGGCAGAAAGTGGTTCGGAACCGAT
ATTAATAAACAGAAACCGAGTTGTTTATAATGGTCATTTCAAGGACATGG
ACAAAACGGATTCATCTATTTTGATGTACAAAAGCCTGGATAAAAAGACT
ATGAAAGATTTATATCTTGAATACATAAAGCACAAAGAAATTGTTCAGTT
GACTCAGAAAGATAGCAGTCTTCCATCCGTAAATTGTTTCATGAAAATTC
GGTTTCATATTCTTTCCAATGTGGGGGATCCAAATTATGTGTACCCCATG
TTTATTATAAATGGGATAGATTGTTCACTTAGGAAATATATTGACGATTT
CAGATCAGAGATCCCACGGCAATCAGATGAATGTTTATACAATGAAATCT
GTAGGATACTTAAACAAGTTTTGTATGGTTTGGAGTTCCTTCATGAACAA
ACACCCCCTTCAAATCACTATGATCTAACAATAGATTCTATAGGATATAA
CAGTACGCAGAACGCAATAAAATTATCGAGTATATCGAATGAAACAAAGG
TGCTCGTTGATTATAAGCAGACCACGACCAAATATGTCGAAGAAATGTCT
CACTTACCGTTGAAAATATTAGAAGAAAATTCAGAAGTGAAATATTCTAA
AACGACAGATATATATTGTCTAGGAACCATTCTGTATTATGAATGGGTTG
GCACCCTCCCCCTCTTTAAAGAGATATTAGATAAACAAATCAATACCATC
AAAAAATTAGTGGATTATTTACACCTTCATAACATTCAATCTGGTGTTTT
ATCGGGTTGCTTTAAATCAAATTTCGGGAGTAAAATGTTAAAAGCGTGCA
AAATGTGTTGGAAAGACCAGACTATGATCAGGGATTTGAATTTTCTTTTC
AATTAAACTTTTTTCATTTTTCAACTTCTCCTCTTATAAATAAAATTCAT
TAATTTTTACTATTTGAGAAAAAAAATTTAAATTTCTGAAAAAATCAAAA
CAGAATATTTATTTTATGGGAAC
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protein sequence of SMED30021288-orf-1

>SMED30021288-orf-1 ID=SMED30021288-orf-1|Name=SMED30021288-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1029bp
MNPEEIDMEKLTESCIQRFDKESLIIIGDKFEIEDYENIADLKFQILAKI
LTKNQVNQNPEFHTEEIKTIKESVLNEDKARRDFLFDYIEQVENIYKKIQ
RLDKIKVKLSENRIGEKDDPLKSRIKNEFKNFEQRLENIKKTIKVSFKCS
LLVCGDSGSGKSSFINLLVKRDILPVSIRTNTACQVRLTFGGNEYAEVVE
RDGTKKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSILQGGLDI
YDTIGLGESDFFNEHLLKDLSNITGVIFLLKKNNPIGENTVGGLSKIIDH
IMTNDVPSSSMYFVINMCDQLDKSEDIKDLKKNVIKQIKERCVGLNPEVI
TFSTKQEQFLQRQNLMSENYKHLLKHIQDLYINSVNTKTILIYEFLNNFV
WKSVDCIVESEKFTEDDRFEKQDYIRKLKNSVKDHKKSKESFTEKSNDLI
RKYYNNFSKIIKPHFNQQGTRRKVIEEVVQNDYMNFGMSKEEEIEKEINE
KTCNAIMKEFKEFAVKEKMLEKCNGEMLELFLKHYATHLNNKHEYNKIIA
AHIDSTMNRIESDLKITNLNDLLALSNEKRKILSVFAPLIIPVVRMTCTI
KQEFGNLFSFFLSTMNRSNIHIFVNNPKSYIRKKSNVYYKKFFPKHKDTI
INSFLHSTGEATKKFKDYLEESMNDLTTNIFLLEDDMRKRKMVIKTYKDI
KSGLESIRENFCRFYLLHYHKDKYNVMIDQIDSDLKAESGSEPILINRNR
VVYNGHFKDMDKTDSSILMYKSLDKKTMKDLYLEYIKHKEIVQLTQKDSS
LPSVNCFMKIRFHILSNVGDPNYVYPMFIINGIDCSLRKYIDDFRSEIPR
QSDECLYNEICRILKQVLYGLEFLHEQTPPSNHYDLTIDSIGYNSTQNAI
KLSSISNETKVLVDYKQTTTKYVEEMSHLPLKILEENSEVKYSKTTDIYC
LGTILYYEWVGTLPLFKEILDKQINTIKKLVDYLHLHNIQSGVLSGCFKS
NFGSKMLKACKMCWKDQTMIRDLNFLFN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR022812Dynamin_SF
IPR000719Prot_kinase_dom
IPR011009Kinase-like_dom_sf
IPR001024PLAT/LH2_dom
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0004672protein kinase activity
GO:0005515protein binding
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 425..445
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 151..392
e-value: 6.4E-23
score: 83.5
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 839..1017
e-value: 5.3E-12
score: 47.6
NoneNo IPR availablePANTHERPTHR26392MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-RELATEDcoord: 32..957
NoneNo IPR availableCDDcd00180PKccoord: 831..968
e-value: 2.52977E-7
score: 50.3467
IPR022812Dynamin superfamilyPFAMPF00350Dynamin_Ncoord: 152..304
e-value: 3.3E-14
score: 53.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 742..1024
score: 11.609
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 149..380
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 759..968