DNA-directed RNA polymerase subunit

Overview
NameDNA-directed RNA polymerase subunit
Smed IDSMED30021243
Length (bp)494
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA-directed RNA polymerase subunit (SMED30021243) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA-directed RNA polymerase subunit (SMED30021243) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA-directed RNA polymerase subunit (SMED30021243) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA-directed RNA polymerase subunit (SMED30021243) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021243

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30021243h1SMcG0003729 SmedASXL_004117SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30021243h1SMcG0003729 dd_Smed_v4_3867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR2I (RNA polymerase II subunit I [Source:HGNC Symbol;Acc:HGNC:9196])

HSP 1 Score: 167.162 bits (422), Expect = 1.581e-53
Identity = 79/114 (69.30%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR2I (RNA polymerase II subunit I [Source:HGNC Symbol;Acc:HGNC:9196])

HSP 1 Score: 108.612 bits (270), Expect = 5.488e-31
Identity = 52/77 (67.53%), Postives = 59/77 (76.62%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPK 315
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C K
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQK 88
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR3K (RNA polymerase III subunit K [Source:HGNC Symbol;Acc:HGNC:14121])

HSP 1 Score: 48.9062 bits (115), Expect = 1.503e-7
Identity = 31/112 (27.68%), Postives = 48/112 (42.86%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C  C N L  +E +  RC  +AC  C Y           N+    + E+  +         +  T E  CPKC++  A F Q Q+   ++ M  +Y C N  C HRW +
Sbjct:    2 LLFCPGCGNGLIVEEGQ--RCHRFACNTCPYVHNITRK--VTNRKYPKLKEVDDVLGGAAAWENVDSTAES-CPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Match: rpb-9 (DNA-directed RNA polymerase subunit [Source:UniProtKB/TrEMBL;Acc:Q965S0])

HSP 1 Score: 157.532 bits (397), Expect = 1.166e-49
Identity = 66/114 (57.89%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYP+EDKE+R L+Y+CRNC + ++A   CIYVNK+   +DEL+QI  D+IHDPTL +TEEH CP C   +AVFFQ+Q+ + E+ MRLYYVC +++C HRW+E
Sbjct:   54 VGIKFCPECNNMLYPREDKESRVLMYSCRNCEHREVAANPCIYVNKLVHEIDELTQIVGDIIHDPTLPKTEEHQCPVCGKSKAVFFQAQTKKAEEEMRLYYVCASQDCQHRWTE 167
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Match: rpc-11 (DNA-directed RNA polymerase subunit [Source:UniProtKB/TrEMBL;Acc:U4PMG8])

HSP 1 Score: 43.8986 bits (102), Expect = 3.186e-6
Identity = 25/115 (21.74%), Postives = 49/115 (42.61%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTL---SRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C EC  +L  + +   +C+ ++C  C Y       C     +   +    +   DV+  P     ++  +  CP C +  A F Q Q+   ++   ++Y C +  C HRW +
Sbjct:    2 LTFCPECGCVL--QIESGEQCMRFSCPACPY------VCPVTQTVTSRIYPKLKDIDDVLGGPGAWANAQVTDETCPVCSHGRAYFMQLQTRSADEPSTIFYRCADNACAHRWKD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Match: RpII15 (gene:FBgn0004855 transcript:FBtr0346348)

HSP 1 Score: 168.703 bits (426), Expect = 1.941e-54
Identity = 76/114 (66.67%), Postives = 92/114 (80.70%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE + LLYACRNC+Y Q A+  CIYVNKI   +DEL+ I  DVI DPTL RTE+H CPKC ++EAVFFQ+Q+ R E+ MRLYYVCTN+NC HRW+E
Sbjct:   16 VGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE 129
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Match: RpII15 (gene:FBgn0004855 transcript:FBtr0082925)

HSP 1 Score: 168.703 bits (426), Expect = 1.941e-54
Identity = 76/114 (66.67%), Postives = 92/114 (80.70%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE + LLYACRNC+Y Q A+  CIYVNKI   +DEL+ I  DVI DPTL RTE+H CPKC ++EAVFFQ+Q+ R E+ MRLYYVCTN+NC HRW+E
Sbjct:   16 VGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE 129
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Match: RpII15 (gene:FBgn0004855 transcript:FBtr0309995)

HSP 1 Score: 168.703 bits (426), Expect = 1.941e-54
Identity = 76/114 (66.67%), Postives = 92/114 (80.70%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE + LLYACRNC+Y Q A+  CIYVNKI   +DEL+ I  DVI DPTL RTE+H CPKC ++EAVFFQ+Q+ R E+ MRLYYVCTN+NC HRW+E
Sbjct:   16 VGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE 129
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Match: CG33785 (gene:FBgn0061362 transcript:FBtr0091786)

HSP 1 Score: 43.8986 bits (102), Expect = 4.765e-6
Identity = 31/111 (27.93%), Postives = 48/111 (43.24%), Query Frame = 1 
Query:   97 YCNECNNMLYPKEDKETRCLLYACRNCNY-SQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            +C  C N+L  +ED  T C  + C  C Y S+I  +      K    + E+  +         +  T+   CP C +K A F Q Q+   ++ M  +Y C N  C H W +
Sbjct:    4 FCPSCGNILIIEED--TNCHRFTCNTCPYISKIRRKI---STKTFPRLKEVDHVLGGKAAWENVDSTDAE-CPTCGHKRAYFMQIQTRSADEPMTTFYKCCNHECNHTWRD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Match: polr2i (polymerase (RNA) II (DNA directed) polypeptide I [Source:ZFIN;Acc:ZDB-GENE-041010-106])

HSP 1 Score: 167.933 bits (424), Expect = 4.722e-54
Identity = 78/114 (68.42%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H CPKC +KEAVFFQS S + ED+MRLYYVCT  +C HRW+E
Sbjct:   13 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVAQDPTLPRTEDHPCPKCGHKEAVFFQSHSMKAEDAMRLYYVCTAPHCGHRWTE 126
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Match: polr2i (polymerase (RNA) II (DNA directed) polypeptide I [Source:ZFIN;Acc:ZDB-GENE-041010-106])

HSP 1 Score: 167.933 bits (424), Expect = 4.722e-54
Identity = 78/114 (68.42%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H CPKC +KEAVFFQS S + ED+MRLYYVCT  +C HRW+E
Sbjct:   13 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVAQDPTLPRTEDHPCPKCGHKEAVFFQSHSMKAEDAMRLYYVCTAPHCGHRWTE 126
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Match: polr3k (polymerase (RNA) III (DNA directed) polypeptide K [Source:ZFIN;Acc:ZDB-GENE-040718-291])

HSP 1 Score: 47.7506 bits (112), Expect = 2.286e-7
Identity = 31/112 (27.68%), Postives = 49/112 (43.75%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C  C N+L  +E +  RC  +AC  C Y           N+    + E+  +         +  T E  CPKC++  A F Q Q+   ++ M  +Y C N  C HRW +
Sbjct:    2 LLFCPTCGNVLIVEEGQ--RCFRFACNTCPYVHNITRK--VNNRKYPKLKEVDDVLGGSAAWENVDSTAEP-CPKCEHPRAYFMQIQTRSADEPMTTFYKCCNIQCGHRWRD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: slc25a3 (solute carrier family 25 member 3 [Source:Xenbase;Acc:XB-GENE-489634])

HSP 1 Score: 137.502 bits (345), Expect = 8.957e-42
Identity = 66/107 (61.68%), Postives = 80/107 (74.77%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNEN 405
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   +DEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR E  +R++++    N
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEIDELTQIIADVAQDPTLPRTEDHPCSKCGHKEAVFFQSHSARAE--VRIFFILHKTN 116
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: slc25a3 (solute carrier family 25 member 3 [Source:Xenbase;Acc:XB-GENE-489634])

HSP 1 Score: 128.642 bits (322), Expect = 2.613e-38
Identity = 61/94 (64.89%), Postives = 71/94 (75.53%), Query Frame = 1 
Query:  148 RCLLY-ACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            +CL Y  CRNC+Y Q A+  CIYVNKI   +DEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:   40 KCLFYLQCRNCDYQQEADNSCIYVNKITHEIDELTQIIADVAQDPTLPRTEDHPCSKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 133
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: csgalnact1 (chondroitin sulfate N-acetylgalactosaminyltransferase 1 [Source:Xenbase;Acc:XB-GENE-976931])

HSP 1 Score: 45.8246 bits (107), Expect = 1.968e-6
Identity = 29/112 (25.89%), Postives = 49/112 (43.75%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C  C N+L  +E +  +C  +AC  C Y           ++    + E+  +         +  T E  CPKC++  A F Q Q+   ++ M  +Y C N  C HRW +
Sbjct:    2 LLFCPTCGNVLIVEEGQ--KCYRFACNTCPYVHNVNRK--VTSRKYPKLKEVDDVLGGSAAWENVDSTAE-TCPKCEHPRAYFMQIQTRSADEPMTTFYKCCNIQCGHRWRD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr2i (polymerase (RNA) II (DNA directed) polypeptide I [Source:MGI Symbol;Acc:MGI:1917170])

HSP 1 Score: 167.162 bits (422), Expect = 1.085e-53
Identity = 79/114 (69.30%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr2i (polymerase (RNA) II (DNA directed) polypeptide I [Source:MGI Symbol;Acc:MGI:1917170])

HSP 1 Score: 152.525 bits (384), Expect = 3.416e-48
Identity = 73/103 (70.87%), Postives = 81/103 (78.64%), Query Frame = 1 
Query:  118 MLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            MLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:    1 MLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 103
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr2i (polymerase (RNA) II (DNA directed) polypeptide I [Source:MGI Symbol;Acc:MGI:1917170])

HSP 1 Score: 119.783 bits (299), Expect = 1.304e-35
Identity = 59/83 (71.08%), Postives = 64/83 (77.11%), Query Frame = 1 
Query:  118 MLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDE 366
            MLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR E
Sbjct:    1 MLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAE 83
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr2i (polymerase (RNA) II (DNA directed) polypeptide I [Source:MGI Symbol;Acc:MGI:1917170])

HSP 1 Score: 49.2914 bits (116), Expect = 7.744e-8
Identity = 20/27 (74.07%), Postives = 23/27 (85.19%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYA 165
            + I++C ECNNMLYPKEDKE R LLYA
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYA 38
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr3k (polymerase (RNA) III (DNA directed) polypeptide K [Source:MGI Symbol;Acc:MGI:1914255])

HSP 1 Score: 48.521 bits (114), Expect = 1.110e-7
Identity = 31/112 (27.68%), Postives = 48/112 (42.86%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C  C N L  +E +  RC  +AC  C Y           N+    + E+  +         +  T E  CPKC++  A F Q Q+   ++ M  +Y C N  C HRW +
Sbjct:    2 LLFCPGCGNGLIVEEGQ--RCHRFACNTCPYVHNITRK--VTNRKYPKLKEVDDVLGGAAAWENVDSTAE-PCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|P36958|RPB9_DROME (DNA-directed RNA polymerase II subunit RPB9 OS=Drosophila melanogaster OX=7227 GN=RpII15 PE=2 SV=2)

HSP 1 Score: 168.703 bits (426), Expect = 1.946e-53
Identity = 76/114 (66.67%), Postives = 92/114 (80.70%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE + LLYACRNC+Y Q A+  CIYVNKI   +DEL+ I  DVI DPTL RTE+H CPKC ++EAVFFQ+Q+ R E+ MRLYYVCTN+NC HRW+E
Sbjct:   16 VGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE 129
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|P60899|RPB9_PIG (DNA-directed RNA polymerase II subunit RPB9 OS=Sus scrofa OX=9823 GN=POLR2I PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 7.592e-53
Identity = 79/114 (69.30%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|P60898|RPB9_MOUSE (DNA-directed RNA polymerase II subunit RPB9 OS=Mus musculus OX=10090 GN=Polr2i PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 7.592e-53
Identity = 79/114 (69.30%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|P36954|RPB9_HUMAN (DNA-directed RNA polymerase II subunit RPB9 OS=Homo sapiens OX=9606 GN=POLR2I PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 7.592e-53
Identity = 79/114 (69.30%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|Q32P73|RPB9_BOVIN (DNA-directed RNA polymerase II subunit RPB9 OS=Bos taurus OX=9913 GN=POLR2I PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 7.675e-53
Identity = 79/114 (69.30%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H C KC +KEAVFFQS SAR ED+MRLYYVCT  +C HRW+E
Sbjct:   12 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A293LPG2 (DNA-directed RNA polymerase subunit (Fragment) OS=Ornithodoros erraticus OX=265619 PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.409e-56
Identity = 81/114 (71.05%), Postives = 95/114 (83.33%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE+R LLYACRNC+Y Q+A+  CIYVNKI   VDEL+QI +DV+HDPTL RT+EH CPKC N+EAVFFQ+QS R ED MRLYYVCTN  C HRW+E
Sbjct:   15 VGIKFCQECNNMLYPKEDKESRVLLYACRNCDYQQVADNNCIYVNKITHEVDELTQIVSDVVHDPTLPRTDEHTCPKCGNREAVFFQAQSRRAEDEMRLYYVCTNTTCTHRWTE 128
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A4D5S2H3 (Putative rna polymerase ii subunit (Fragment) OS=Ixodes scapularis OX=6945 PE=3 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 7.145e-56
Identity = 81/114 (71.05%), Postives = 95/114 (83.33%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q+A+  CIYVNKI   VDEL+QI +DV+HDPTL RTE+H CPKC ++EAVFFQ+QS R ED MRLYYVCTN NC HRW+E
Sbjct:   50 VGIKFCQECNNMLYPKEDKENRVLLYACRNCDYQQVADNNCIYVNKITHEVDELTQIVSDVVHDPTLPRTEDHTCPKCSHREAVFFQAQSRRAEDEMRLYYVCTNTNCTHRWTE 163
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: B7PRG9 (DNA-directed RNA polymerase subunit OS=Ixodes scapularis OX=6945 GN=8030285 PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 7.757e-56
Identity = 81/114 (71.05%), Postives = 95/114 (83.33%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q+A+  CIYVNKI   VDEL+QI +DV+HDPTL RTE+H CPKC ++EAVFFQ+QS R ED MRLYYVCTN NC HRW+E
Sbjct:   16 VGIKFCQECNNMLYPKEDKENRVLLYACRNCDYQQVADNNCIYVNKITHEVDELTQIVSDVVHDPTLPRTEDHTCPKCSHREAVFFQAQSRRAEDEMRLYYVCTNTNCTHRWTE 129
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A0K8RCV5 (DNA-directed RNA polymerase subunit OS=Ixodes ricinus OX=34613 PE=2 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 7.757e-56
Identity = 81/114 (71.05%), Postives = 95/114 (83.33%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q+A+  CIYVNKI   VDEL+QI +DV+HDPTL RTE+H CPKC ++EAVFFQ+QS R ED MRLYYVCTN NC HRW+E
Sbjct:   16 VGIKFCQECNNMLYPKEDKENRVLLYACRNCDYQQVADNNCIYVNKITHEVDELTQIVSDVVHDPTLPRTEDHTCPKCSHREAVFFQAQSRRAEDEMRLYYVCTNTNCTHRWTE 129
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A2R5LG37 (DNA-directed RNA polymerase subunit OS=Ornithodoros turicata OX=34597 PE=3 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 1.229e-55
Identity = 81/114 (71.05%), Postives = 94/114 (82.46%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q+A+  CIYVNKI   VDEL+QI +DV+HDPTL RT+EH CPKC N+EAVFFQ+QS R ED MRLYYVCTN  C HRW+E
Sbjct:   15 VGIKFCQECNNMLYPKEDKENRVLLYACRNCDYQQVADNNCIYVNKITHEVDELTQIVSDVVHDPTLPRTDEHTCPKCGNREAVFFQAQSRRAEDEMRLYYVCTNATCTHRWTE 128
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Match: polr2i (RNA polymerase II subunit I [Source:NCBI gene;Acc:103024975])

HSP 1 Score: 167.548 bits (423), Expect = 6.405e-54
Identity = 78/114 (68.42%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H CPKC +KEAVFFQS S + ED+MRLYYVCT  +C HRW+E
Sbjct:   13 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCPKCGHKEAVFFQSHSMKAEDAMRLYYVCTAPHCGHRWTE 126
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Match: polr3k (RNA polymerase III subunit K [Source:NCBI gene;Acc:103032815])

HSP 1 Score: 45.4394 bits (106), Expect = 1.297e-6
Identity = 30/113 (26.55%), Postives = 51/113 (45.13%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNY-SQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C  C N+L  +E +  +C  +AC  C Y   I  +     N+    + E+  +         +  T E  CPKC++  A F Q Q+   ++ M  +Y C +  C HRW +
Sbjct:    2 LLFCPACGNVLIVEEGQ--KCYRFACNTCPYIHNITRKV---NNRKYPKLKEVDDVLGGAAAWENVDSTAE-TCPKCEHPRAYFMQIQTRSADEPMTTFYKCCSPQCGHRWRD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Yeast
Match: RPB9 (RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription [Source:SGD;Acc:S000003038])

HSP 1 Score: 97.4413 bits (241), Expect = 2.263e-27
Identity = 44/101 (43.56%), Postives = 67/101 (66.34%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVC 393
             ++C +CNNMLYP+EDKE   LL+ CR C+Y + A    +Y +++  ++ E + +  D+  DPTL R++   CPKC ++E VFFQSQ  R + SM L++VC
Sbjct:    4 FRFCRDCNNMLYPREDKENNRLLFECRTCSYVEEAGSPLVYRHELITNIGETAGVVQDIGSDPTLPRSDRE-CPKCHSRENVFFQSQQRRKDTSMVLFFVC 103
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Yeast
Match: RPC11 (RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS [Source:SGD;Acc:S000002452])

HSP 1 Score: 45.4394 bits (106), Expect = 1.677e-7
Identity = 34/115 (29.57%), Postives = 51/115 (44.35%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPK---CKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C  CNNML      ++     ACR+C Y    E   IY  K ++   E+  +      +   ++T+   CP    C  + A FFQ Q    ++ M  +Y C   NC HRW E
Sbjct:    2 LSFCPSCNNMLL-ITSGDSGVYTLACRSCPYEFPIEGIEIYDRK-KLPRKEVDDVLGGGWDNVDQTKTQ---CPNYDTCGGESAYFFQLQIRSADEPMTTFYKCV--NCGHRWKE 109
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Match: EDO31597 (DNA-directed RNA polymerase subunit [Source:UniProtKB/TrEMBL;Acc:A7SXH3])

HSP 1 Score: 154.836 bits (390), Expect = 2.207e-49
Identity = 73/127 (57.48%), Postives = 94/127 (74.02%), Query Frame = 1 
Query:   46 LIMSRSEEQDVKKLKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            +  S S       + I++C ECNNMLYPKEDK+ + LLYACRNC+Y Q A+  CIYVNKI   V+EL+QI  DVI DPTL RTE+H CP+C ++E+VFFQSQS++  D M LYYVCT+ +C H+W+E
Sbjct:    4 MFRSNSRSDGPGFVGIKFCQECNNMLYPKEDKDHKILLYACRNCDYQQEADNSCIYVNKITHEVNELTQIVGDVIADPTLPRTEDHECPRCGHRESVFFQSQSSK-ADQMVLYYVCTSVDCGHKWTE 129
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Match: polr2i (RNA polymerase II subunit I [Source:NCBI gene;Acc:101158787])

HSP 1 Score: 168.318 bits (425), Expect = 2.772e-54
Identity = 78/114 (68.42%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:   85 LKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + I++C ECNNMLYPKEDKE R LLYACRNC+Y Q A+  CIYVNKI   VDEL+QI  DV  DPTL RTE+H CPKC +KEAVFFQS S + ED+MRLYYVCT  +C HRW+E
Sbjct:   13 VGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVAQDPTLPRTEDHPCPKCGHKEAVFFQSHSMKAEDAMRLYYVCTAPHCGHRWTE 126
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Match: polr3k (RNA polymerase III subunit K [Source:NCBI gene;Acc:101173392])

HSP 1 Score: 47.3654 bits (111), Expect = 2.766e-7
Identity = 30/112 (26.79%), Postives = 50/112 (44.64%), Query Frame = 1 
Query:   91 IQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            + +C  C N+L  +E +  +CL +AC  C Y         Y    ++   E+  +         +  T E  CPKC++  A F Q Q+   ++ M  +Y C N  C +RW +
Sbjct:    2 LLFCPTCGNVLIVEEGQ--KCLRFACNTCPYVHNVTRKVNYRKYPKLK--EVDDVLGGAAAWENVDSTAE-TCPKCEHPRAYFMQIQTRSADEPMTTFYKCCNAQCGNRWRD 108
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Match: SMESG000080671.1 (SMESG000080671.1)

HSP 1 Score: 256.914 bits (655), Expect = 2.288e-89
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 1 
Query:   52 MSRSEEQDVKKLKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            MSRSEEQDVKKLKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE
Sbjct:    1 MSRSEEQDVKKLKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 125
[Close]
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Match: SMESG000065228.1 (SMESG000065228.1)

HSP 1 Score: 219.935 bits (559), Expect = 7.525e-75
Identity = 105/125 (84.00%), Postives = 118/125 (94.40%), Query Frame = 1 
Query:   52 MSRSEEQDVKKLKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEEYCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQSARDEDSMRLYYVCTNENCLHRWSE 426
            MSRSEE+ VKKLKI+YCNECNN+LYPKEDKETRCLL ACR CNY++IA++YCIY NKIEMSVDELSQICTDVI+DPTLSRTE+H CPKCK+ EAVFFQSQSARDEDSMRLYYVCT ENCL++W+E
Sbjct:    1 MSRSEERVVKKLKIKYCNECNNLLYPKEDKETRCLLNACRTCNYTEIADDYCIYSNKIEMSVDELSQICTDVIYDPTLSRTEDHSCPKCKHNEAVFFQSQSARDEDSMRLYYVCTKENCLYKWTE 125
The following BLAST results are available for this feature:
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
POLR2I1.581e-5369.30RNA polymerase II subunit I [Source:HGNC Symbol;Ac... [more]
POLR2I5.488e-3167.53RNA polymerase II subunit I [Source:HGNC Symbol;Ac... [more]
POLR3K1.503e-727.68RNA polymerase III subunit K [Source:HGNC Symbol;A... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpb-91.166e-4957.89DNA-directed RNA polymerase subunit [Source:UniPr... [more]
rpc-113.186e-621.74DNA-directed RNA polymerase subunit [Source:UniPr... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
RpII151.941e-5466.67gene:FBgn0004855 transcript:FBtr0346348[more]
RpII151.941e-5466.67gene:FBgn0004855 transcript:FBtr0082925[more]
RpII151.941e-5466.67gene:FBgn0004855 transcript:FBtr0309995[more]
CG337854.765e-627.93gene:FBgn0061362 transcript:FBtr0091786[more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
polr2i4.722e-5468.42polymerase (RNA) II (DNA directed) polypeptide I [... [more]
polr2i4.722e-5468.42polymerase (RNA) II (DNA directed) polypeptide I [... [more]
polr3k2.286e-727.68polymerase (RNA) III (DNA directed) polypeptide K ... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
slc25a38.957e-4261.68solute carrier family 25 member 3 [Source:Xenbase;... [more]
slc25a32.613e-3864.89solute carrier family 25 member 3 [Source:Xenbase;... [more]
csgalnact11.968e-625.89chondroitin sulfate N-acetylgalactosaminyltransfer... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Polr2i1.085e-5369.30polymerase (RNA) II (DNA directed) polypeptide I [... [more]
Polr2i3.416e-4870.87polymerase (RNA) II (DNA directed) polypeptide I [... [more]
Polr2i1.304e-3571.08polymerase (RNA) II (DNA directed) polypeptide I [... [more]
Polr2i7.744e-874.07polymerase (RNA) II (DNA directed) polypeptide I [... [more]
Polr3k1.110e-727.68polymerase (RNA) III (DNA directed) polypeptide K ... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P36958|RPB9_DROME1.946e-5366.67DNA-directed RNA polymerase II subunit RPB9 OS=Dro... [more]
sp|P60899|RPB9_PIG7.592e-5369.30DNA-directed RNA polymerase II subunit RPB9 OS=Sus... [more]
sp|P60898|RPB9_MOUSE7.592e-5369.30DNA-directed RNA polymerase II subunit RPB9 OS=Mus... [more]
sp|P36954|RPB9_HUMAN7.592e-5369.30DNA-directed RNA polymerase II subunit RPB9 OS=Hom... [more]
sp|Q32P73|RPB9_BOVIN7.675e-5369.30DNA-directed RNA polymerase II subunit RPB9 OS=Bos... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A293LPG25.409e-5671.05DNA-directed RNA polymerase subunit (Fragment) OS=... [more]
A0A4D5S2H37.145e-5671.05Putative rna polymerase ii subunit (Fragment) OS=I... [more]
B7PRG97.757e-5671.05DNA-directed RNA polymerase subunit OS=Ixodes scap... [more]
A0A0K8RCV57.757e-5671.05DNA-directed RNA polymerase subunit OS=Ixodes rici... [more]
A0A2R5LG371.229e-5571.05DNA-directed RNA polymerase subunit OS=Ornithodoro... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
polr2i6.405e-5468.42RNA polymerase II subunit I [Source:NCBI gene;Acc:... [more]
polr3k1.297e-626.55RNA polymerase III subunit K [Source:NCBI gene;Acc... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPB92.263e-2743.56RNA polymerase II subunit B12.6; contacts DNA; mut... [more]
RPC111.677e-729.57RNA polymerase III subunit C11; mediates pol III R... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO315972.207e-4957.48DNA-directed RNA polymerase subunit [Source:UniPr... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
polr2i2.772e-5468.42RNA polymerase II subunit I [Source:NCBI gene;Acc:... [more]
polr3k2.766e-726.79RNA polymerase III subunit K [Source:NCBI gene;Acc... [more]
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BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000080671.12.288e-89100.00SMESG000080671.1[more]
SMESG000065228.17.525e-7584.00SMESG000065228.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021243 ID=SMED30021243|Name=DNA-directed RNA polymerase subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=494bp
GGGTAAAGCAGATAGTTTGTTTCAACATTTTGTTTTGTTTTAATTTTAAT
TATGTCAAGATCTGAAGAGCAAGATGTCAAGAAATTAAAAATACAATATT
GTAATGAGTGCAATAATATGCTTTACCCAAAAGAAGATAAAGAAACAAGA
TGTTTGTTATATGCCTGCAGGAATTGCAATTACAGCCAAATAGCAGAGGA
ATATTGCATCTATGTTAATAAAATAGAAATGAGTGTAGATGAATTATCAC
AAATATGTACGGATGTAATTCATGATCCAACTCTTTCAAGAACTGAAGAG
CATTTATGTCCTAAATGTAAAAACAAGGAGGCTGTTTTTTTCCAATCGCA
ATCTGCAAGGGACGAGGACTCTATGAGACTTTATTATGTTTGCACGAATG
AAAATTGTTTGCATAGATGGTCTGAATAAAATTTGTTTATATATATAATC
TTTTAGACATGTATTTTTCTATGTTCTAAATATTACTACTGTGG
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protein sequence of SMED30021243-orf-1

>SMED30021243-orf-1 ID=SMED30021243-orf-1|Name=SMED30021243-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=126bp
MSRSEEQDVKKLKIQYCNECNNMLYPKEDKETRCLLYACRNCNYSQIAEE
YCIYVNKIEMSVDELSQICTDVIHDPTLSRTEEHLCPKCKNKEAVFFQSQ
SARDEDSMRLYYVCTNENCLHRWSE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR034012Zn_ribbon_RPB9_C
IPR001529DNA-dir_RNA_pol_M/15kDasu
IPR001222Znf_TFIIS
IPR012164Rpa12/Rpb9/Rpc10/TFS
IPR019761DNA-dir_RNA_pol-M_15_CS
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
GO:0006379mRNA cleavage
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0003676nucleic acid binding
GO:0003899DNA-directed 5'-3' RNA polymerase activity
GO:0016740transferase activity
GO:0016779nucleotidyltransferase activity
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005730nucleolus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001529DNA-directed RNA polymerase, M/15kDa subunitSMARTSM00661rpol9cneucoord: 15..68
e-value: 1.7E-14
score: 64.1
IPR001529DNA-directed RNA polymerase, M/15kDa subunitPFAMPF02150RNA_POL_M_15KDcoord: 14..46
e-value: 7.1E-11
score: 41.8
IPR001222Zinc finger, TFIIS-typeSMARTSM00440Cys4_2coord: 84..125
e-value: 5.6E-11
score: 52.5
IPR001222Zinc finger, TFIIS-typePFAMPF01096TFIIS_Ccoord: 85..123
e-value: 4.1E-11
score: 42.5
IPR001222Zinc finger, TFIIS-typePROSITEPS00466ZF_TFIIS_1coord: 86..123
IPR001222Zinc finger, TFIIS-typePROSITEPS51133ZF_TFIIS_2coord: 82..124
score: 12.1
IPR012164DNA-directed RNA polymerase subunit/transcription factor SPIRSFPIRSF005586RNApol_RpoMcoord: 14..125
e-value: 4.0E-16
score: 57.2
NoneNo IPR availableGENE3DG3DSA:2.20.25.10coord: 57..125
e-value: 4.6E-26
score: 92.4
NoneNo IPR availableGENE3DG3DSA:2.20.25.10coord: 1..56
e-value: 3.3E-21
score: 76.5
NoneNo IPR availablePANTHERPTHR11239DNA-DIRECTED RNA POLYMERASEcoord: 11..125
NoneNo IPR availablePANTHERPTHR11239:SF1DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9coord: 11..125
NoneNo IPR availableSUPERFAMILYSSF57783Zinc beta-ribboncoord: 13..58
NoneNo IPR availableSUPERFAMILYSSF57783Zinc beta-ribboncoord: 61..125
IPR019761DNA-directed RNA polymerase M, 15kDa subunit, conserved sitePROSITEPS01030RNA_POL_M_15KDcoord: 16..42
IPR034012Pol II subunit B9, C-terminal zinc ribbonCDDcd10508Zn-ribbon_RPB9coord: 74..124
e-value: 8.26161E-23
score: 81.9158