SMED30021189

Overview


Name	SMED30021189
Smed ID	SMED30021189
Length (bp)	844

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30021189 (SMED30021189) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021189 (SMED30021189) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021189 (SMED30021189) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021189 (SMED30021189) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30021189

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30021189	h1SMcG0021780	dd_Smed_v4_1635_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30021189	h1SMcG0021780	dd_Smed_v4_1635_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30021189	h1SMcG0021780	dd_Smed_v4_1635_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30021189	h1SMcG0021780	dd_Smed_v4_1635_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30021189	h1SMcG0021780	dd_Smed_v4_1635_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30021189 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000153:80504..82512 -	3786

Homology

[Close]

BLAST of SMED30021189 vs. Ensembl Human
Match: GNAO1 (G protein subunit alpha o1 [Source:HGNC Symbol;Acc:HGNC:4389])

HSP 1 Score: 51.6026 bits (122), Expect = 3.095e-8
Identity = 33/68 (48.53%), Postives = 45/68 (66.18%), Query Frame = -1

Query:  593 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKFRKNPPGYPLS 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K       YPLS
Sbjct:    1 MGCTLSAEERAALERSKAIEKNLKEDGIS---AAKDVKLLLLGAGESGKSTIVKQMK-------YPLS 58

[Close]

BLAST of SMED30021189 vs. Ensembl Human
Match: GNAO1 (G protein subunit alpha o1 [Source:HGNC Symbol;Acc:HGNC:4389])

HSP 1 Score: 49.2914 bits (116), Expect = 9.080e-8
Identity = 29/57 (50.88%), Postives = 41/57 (71.93%), Query Frame = -1

Query:  626 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIK 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K
Sbjct:    1 MGCTLSAEERAALERSKAIEKNLKEDGIS---AAKDVKLLLLGAGESGKSTIVKQMK 54

[Close]

BLAST of SMED30021189 vs. Ensembl Human
Match: GNAO1 (G protein subunit alpha o1 [Source:HGNC Symbol;Acc:HGNC:4389])

HSP 1 Score: 50.0618 bits (118), Expect = 1.868e-7
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALERSKAIEKNLKEDGIS---AAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Human
Match: GNAI1 (G protein subunit alpha i1 [Source:HGNC Symbol;Acc:HGNC:4384])

HSP 1 Score: 50.8322 bits (120), Expect = 1.978e-7
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+ERSK ID++L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEDKAAVERSKMIDRNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Human
Match: GNAO1 (G protein subunit alpha o1 [Source:HGNC Symbol;Acc:HGNC:4389])

HSP 1 Score: 50.447 bits (119), Expect = 2.903e-7
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALERSKAIEKNLKEDGI---SAAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Celegans
Match: goa-1 (Guanine nucleotide-binding protein G(o) subunit alpha [Source:UniProtKB/Swiss-Prot;Acc:P51875])

HSP 1 Score: 48.521 bits (114), Expect = 2.349e-6
Identity = 28/58 (48.28%), Postives = 42/58 (72.41%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERS+ I+K+LKEDG+Q     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTMSQEERAALERSRMIEKNLKEDGMQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Fly
Match: Galphao (gene:FBgn0001122 transcript:FBtr0088267)

HSP 1 Score: 51.2174 bits (121), Expect = 4.039e-7
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC  S E++ AI+RSK I+K+LKEDGIQ     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTTSAEERAAIQRSKQIEKNLKEDGIQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Fly
Match: Galphao (gene:FBgn0001122 transcript:FBtr0088269)

HSP 1 Score: 51.2174 bits (121), Expect = 4.039e-7
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC  S E++ AI+RSK I+K+LKEDGIQ     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTTSAEERAAIQRSKQIEKNLKEDGIQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Fly
Match: Galphao (gene:FBgn0001122 transcript:FBtr0088264)

HSP 1 Score: 51.2174 bits (121), Expect = 4.039e-7
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC  S E++ AI+RSK I+K+LKEDGIQ     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTTSAEERAAIQRSKQIEKNLKEDGIQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Fly
Match: Galphao (gene:FBgn0001122 transcript:FBtr0088268)

HSP 1 Score: 51.2174 bits (121), Expect = 4.039e-7
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC  S E++ AI+RSK I+K+LKEDGIQ     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTTSAEERAAIQRSKQIEKNLKEDGIQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Fly
Match: Galphao (gene:FBgn0001122 transcript:FBtr0088270)

HSP 1 Score: 51.2174 bits (121), Expect = 4.039e-7
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC  S E++ AI+RSK I+K+LKEDGIQ     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTTSAEERAAIQRSKQIEKNLKEDGIQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Zebrafish
Match: gnai2b (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5861])

HSP 1 Score: 50.0618 bits (118), Expect = 1.397e-6
Identity = 30/58 (51.72%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERSK IDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSAEDKAAAERSKMIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Zebrafish
Match: gnai2a (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2a [Source:ZFIN;Acc:ZDB-GENE-030131-8365])

HSP 1 Score: 49.2914 bits (116), Expect = 2.520e-6
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+ERSK ID++L+EDG    K  K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSQEDKAALERSKMIDRNLREDG---EKAAKEVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Zebrafish
Match: gnai1 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1310])

HSP 1 Score: 48.521 bits (114), Expect = 3.615e-6
Identity = 30/66 (45.45%), Postives = 44/66 (66.67%), Query Frame = -1

Query:  599 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKFRKNPPGYP 796
            MGC +S EDK A+ERSK ID++L++DG    K  + + + ++G  +SGKSTI KQ+K   +  GY 
Sbjct:    1 MGCTLSTEDKAAVERSKMIDRNLRDDG---EKAAREVKLLLLGAGESGKSTIVKQMKI-IHEAGYS 62

[Close]

BLAST of SMED30021189 vs. Ensembl Zebrafish
Match: gnai3 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 [Source:ZFIN;Acc:ZDB-GENE-070713-6])

HSP 1 Score: 48.1358 bits (113), Expect = 5.243e-6
Identity = 28/58 (48.28%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+E+SK ID++L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSTEDKAAMEKSKMIDRTLREDG---EKASREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Zebrafish
Match: gnao1a (guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O, a [Source:ZFIN;Acc:ZDB-GENE-040426-1757])

HSP 1 Score: 48.1358 bits (113), Expect = 5.387e-6
Identity = 28/58 (48.28%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A++RSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALDRSKAIEKNLKEDGI---TAAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Xenopus
Match: gnai2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 [Source:NCBI gene;Acc:394861])

HSP 1 Score: 53.1434 bits (126), Expect = 1.626e-7
Identity = 31/58 (53.45%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERSKNIDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSAEDKAAAERSKNIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Xenopus
Match: gnai2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 [Source:NCBI gene;Acc:394861])

HSP 1 Score: 53.1434 bits (126), Expect = 1.895e-7
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = -1

Query:  626 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIK 796
            MGC +S EDK A ERSKNIDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K
Sbjct:    1 MGCTVSAEDKAAAERSKNIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMK 54

[Close]

BLAST of SMED30021189 vs. Ensembl Xenopus
Match: gnao1 (G protein subunit alpha o1 [Source:NCBI gene;Acc:549749])

HSP 1 Score: 50.447 bits (119), Expect = 1.526e-6
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALERSKQIEKNLKEDGIS---AAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Xenopus
Match: gnao1 (G protein subunit alpha o1 [Source:NCBI gene;Acc:549749])

HSP 1 Score: 50.0618 bits (118), Expect = 1.597e-6
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALERSKQIEKNLKEDGIS---AAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Xenopus
Match: gnai1 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 [Source:NCBI gene;Acc:100038283])

HSP 1 Score: 50.447 bits (119), Expect = 1.639e-6
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+ERSK ID++L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:   44 MGCTLSAEDKAAVERSKMIDRNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 98

[Close]

BLAST of SMED30021189 vs. Ensembl Mouse
Match: Gnai3 (guanine nucleotide binding protein (G protein), alpha inhibiting 3 [Source:MGI Symbol;Acc:MGI:95773])

HSP 1 Score: 50.0618 bits (118), Expect = 1.254e-6
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+ERSK ID++L+EDG    K  K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEDKAAVERSKMIDRNLREDG---EKAAKEVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Mouse
Match: Gnai1 (guanine nucleotide binding protein (G protein), alpha inhibiting 1 [Source:MGI Symbol;Acc:MGI:95771])

HSP 1 Score: 50.0618 bits (118), Expect = 1.313e-6
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+ERSK ID++L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEDKAAVERSKMIDRNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Mouse
Match: Gnai2 (guanine nucleotide binding protein (G protein), alpha inhibiting 2 [Source:MGI Symbol;Acc:MGI:95772])

HSP 1 Score: 50.0618 bits (118), Expect = 1.454e-6
Identity = 30/58 (51.72%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERSK IDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSAEDKAAAERSKMIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERSK IDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSAEDKAAAERSKMIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Mouse
Match: Gnao1 (guanine nucleotide binding protein, alpha O [Source:MGI Symbol;Acc:MGI:95775])

HSP 1 Score: 49.6766 bits (117), Expect = 1.820e-6
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALERSKAIEKNLKEDGIS---AAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. UniProt/SwissProt
Match: sp|O15976|GNAO_MIZYE (Guanine nucleotide-binding protein G(o) subunit alpha OS=Mizuhopecten yessoensis OX=6573 GN=SCGOA PE=2 SV=3)

HSP 1 Score: 51.9878 bits (123), Expect = 2.470e-6
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S ED+ A ERS++I+K LKEDGIQ     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTMSAEDRAAAERSRDIEKKLKEDGIQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. UniProt/SwissProt
Match: sp|P38404|GNAO_LOCMI (Guanine nucleotide-binding protein G(o) subunit alpha OS=Locusta migratoria OX=7004 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 5.467e-6
Identity = 29/57 (50.88%), Postives = 42/57 (73.68%), Query Frame = -1

Query:  626 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIK 796
            MGCA+S E++ A+ RSK I+K+LKEDG+Q     K + + ++G  +SGKSTI KQ+K
Sbjct:    1 MGCAMSAEERAALARSKQIEKNLKEDGLQ---AAKDIKLLLLGAGESGKSTIVKQMK 54

[Close]

BLAST of SMED30021189 vs. UniProt/SwissProt
Match: sp|P30683|GNAO_LYMST (Guanine nucleotide-binding protein G(o) subunit alpha OS=Lymnaea stagnalis OX=6523 PE=2 SV=3)

HSP 1 Score: 50.447 bits (119), Expect = 6.324e-6
Identity = 29/58 (50.00%), Postives = 42/58 (72.41%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERSK I+K+LKEDG+Q     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAAMERSKAIEKNLKEDGMQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. UniProt/SwissProt
Match: sp|P51877|GNAO_PLATR (Guanine nucleotide-binding protein G(o) subunit alpha OS=Planorbella trivolvis OX=283763 PE=2 SV=2)

HSP 1 Score: 50.447 bits (119), Expect = 6.440e-6
Identity = 29/58 (50.00%), Postives = 42/58 (72.41%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A+ERSK I+K+LKEDG+Q     K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAAMERSKAIEKNLKEDGMQ---AAKDIKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. UniProt/SwissProt
Match: sp|Q5RAD4|GNAI1_PONAB (Guanine nucleotide-binding protein G(i) subunit alpha-1 OS=Pongo abelii OX=9601 GN=GNAI1 PE=2 SV=3)

HSP 1 Score: 50.0618 bits (118), Expect = 8.460e-6
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+ERSK ID++L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEDKAAVERSKMIDRNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. TrEMBL
Match: A0A2J8VYP2 (GNAO1 isoform 5 OS=Pongo abelii OX=9601 GN=CR201_G0014568 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 4.003e-6
Identity = 35/72 (48.61%), Postives = 49/72 (68.06%), Query Frame = -1

Query:  590 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKFR---KNPPGYPLSV 796
            MGC +S E++ A+ERSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K+R    + PG  LSV
Sbjct:    1 MGCTLSAEERAALERSKAIEKNLKEDGIS---AAKDVKLLLLGAGESGKSTIVKQMKYRLSFYSVPGTILSV 69

[Close]

BLAST of SMED30021189 vs. Ensembl Cavefish
Match: ENSAMXT00000044204.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000133.1:1325820:1326334:-1 gene:ENSAMXG00000043193.1 transcript:ENSAMXT00000044204.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.1358 bits (113), Expect = 1.641e-7
Identity = 27/57 (47.37%), Postives = 41/57 (71.93%), Query Frame = -1

Query:  626 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIK 796
            MGC +S E++ A++RSK I+K+LKEDG+      K + + ++G  +SGKSTI KQ+K
Sbjct:    1 MGCTLSAEERAALDRSKAIEKNLKEDGL---TAAKEVKLLLLGGGESGKSTIVKQMK 54

[Close]

BLAST of SMED30021189 vs. Ensembl Cavefish
Match: gnai2a (guanine nucleotide-binding protein G(i) subunit alpha-2 [Source:NCBI gene;Acc:103033963])

HSP 1 Score: 50.447 bits (119), Expect = 7.793e-7
Identity = 31/58 (53.45%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERS++IDKSL+EDG    K  K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSQEDKAAAERSRHIDKSLREDG---EKAAKEVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Cavefish
Match: gnai2b (guanine nucleotide-binding protein G(i) subunit alpha-2 [Source:NCBI gene;Acc:111193748])

HSP 1 Score: 50.0618 bits (118), Expect = 1.121e-6
Identity = 30/58 (51.72%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERSK IDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSAEDKAAAERSKMIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Cavefish
Match: gnao1a (G protein subunit alpha o1 [Source:NCBI gene;Acc:103039135])

HSP 1 Score: 48.1358 bits (113), Expect = 4.170e-6
Identity = 28/58 (48.28%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A++RSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALDRSKAIEKNLKEDGI---TAAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Cavefish
Match: gnao1a (G protein subunit alpha o1 [Source:NCBI gene;Acc:103039135])

HSP 1 Score: 48.1358 bits (113), Expect = 4.607e-6
Identity = 28/58 (48.28%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S E++ A++RSK I+K+LKEDGI      K + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEERAALDRSKAIEKNLKEDGI---TAAKDVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Sea Lamprey
Match: gnai3 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 [Source:ZFIN;Acc:ZDB-GENE-070713-6])

HSP 1 Score: 49.2914 bits (116), Expect = 3.705e-7
Identity = 28/58 (48.28%), Postives = 41/58 (70.69%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A+ERS+ ID++L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSTEDKAAVERSRMIDRNLREDG---EKASREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Nematostella
Match: EDO49692 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG04])

HSP 1 Score: 47.3654 bits (111), Expect = 2.949e-6
Identity = 28/58 (48.28%), Postives = 39/58 (67.24%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERSK IDK+L+ DG    +  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTLSAEDKAAAERSKMIDKNLRADG---ERAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Medaka
Match: gnai2b (guanine nucleotide-binding protein G(i) subunit alpha-2 [Source:NCBI gene;Acc:105358374])

HSP 1 Score: 50.0618 bits (118), Expect = 1.065e-6
Identity = 30/58 (51.72%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A ERSK IDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSAEDKAAAERSKMIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Medaka
Match: ENSORLT00000020087.2 (guanine nucleotide-binding protein G(i) subunit alpha-1 [Source:NCBI gene;Acc:101157265])

HSP 1 Score: 48.521 bits (114), Expect = 3.274e-6
Identity = 30/66 (45.45%), Postives = 44/66 (66.67%), Query Frame = -1

Query:  599 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKFRKNPPGYP 796
            MGC +S EDK A+ERSK ID++L++DG    K  + + + ++G  +SGKSTI KQ+K   +  GY 
Sbjct:    1 MGCTLSTEDKAAVERSKMIDRNLRDDG---EKAAREVKLLLLGAGESGKSTIVKQMKI-IHEAGYS 62

[Close]

BLAST of SMED30021189 vs. Ensembl Medaka
Match: gnai3 (guanine nucleotide-binding protein G(k) subunit alpha [Source:NCBI gene;Acc:101158355])

HSP 1 Score: 48.521 bits (114), Expect = 3.617e-6
Identity = 30/58 (51.72%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC IS EDK A+ERSK IDK+L+ED     K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTISAEDKAAVERSKMIDKNLREDR---EKSSREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Medaka
Match: gnai3 (guanine nucleotide-binding protein G(k) subunit alpha [Source:NCBI gene;Acc:101158355])

HSP 1 Score: 48.521 bits (114), Expect = 3.780e-6
Identity = 30/58 (51.72%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC IS EDK A+ERSK IDK+L+ED     K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTISAEDKAAVERSKMIDKNLREDR---EKSSREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Ensembl Medaka
Match: gnai2 (guanine nucleotide-binding protein G(i) subunit alpha 2 [Source:NCBI gene;Acc:100125814])

HSP 1 Score: 47.7506 bits (112), Expect = 6.651e-6
Identity = 29/58 (50.00%), Postives = 40/58 (68.97%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGC +S EDK A +RSK IDK+L+EDG    K  + + + ++G  +SGKSTI KQ+K 
Sbjct:    1 MGCTVSQEDKAAADRSKMIDKNLREDG---EKAAREVKLLLLGAGESGKSTIVKQMKI 55

[Close]

BLAST of SMED30021189 vs. Planmine SMEST
Match: SMESG000081490.1 (SMESG000081490.1)

HSP 1 Score: 72.7886 bits (177), Expect = 9.003e-16
Identity = 48/58 (82.76%), Postives = 51/58 (87.93%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGCAISVEDKIAIERSKNIDKSLKEDGIQF   +K + + IIGTEKSGKSTIFKQI F
Sbjct:    1 MGCAISVEDKIAIERSKNIDKSLKEDGIQF---LKGIKLLIIGTEKSGKSTIFKQINF 55

[Close]

BLAST of SMED30021189 vs. Planmine SMEST
Match: SMESG000081490.1 (SMESG000081490.1)

HSP 1 Score: 72.7886 bits (177), Expect = 7.136e-15
Identity = 48/58 (82.76%), Postives = 51/58 (87.93%), Query Frame = -1

Query:  623 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIKF 796
            MGCAISVEDKIAIERSKNIDKSLKEDGIQF   +K + + IIGTEKSGKSTIFKQI F
Sbjct:    1 MGCAISVEDKIAIERSKNIDKSLKEDGIQF---LKGIKLLIIGTEKSGKSTIFKQINF 55

[Close]

BLAST of SMED30021189 vs. Planmine SMEST
Match: SMESG000035762.1 (SMESG000035762.1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.018e-10
Identity = 31/48 (64.58%), Postives = 33/48 (68.75%), Query Frame = -1

Query:   14 IQSSTARNGYPLLGDVGNVTTGYEIAKGERYERTAKVRSHSGFNALPL 157
            IQ   A +GYP LG V NVT G EIAKGER ERT   RS  GFNALP+
Sbjct:  224 IQLFFASSGYPSLGKVSNVTIGSEIAKGERCERTTLARSQDGFNALPI 271

[Close]

BLAST of SMED30021189 vs. Planmine SMEST
Match: SMESG000056187.1 (SMESG000056187.1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.087e-9
Identity = 29/47 (61.70%), Postives = 34/47 (72.34%), Query Frame = -1

Query:    8 STARNGYPLLGDVGNVTTGYEIAKGERYERTAKVRSHSGFNALPLEG 148
            S + NGYPL  +VG  TTGY IAKGER+  TAK RSHS FNA P++ 
Sbjct:   13 SLSPNGYPLPSNVGIATTGYGIAKGERHIGTAKARSHSCFNAPPIKA 59

[Close]

BLAST of SMED30021189 vs. Planmine SMEST
Match: SMESG000081487.1 (SMESG000081487.1)

HSP 1 Score: 51.6026 bits (122), Expect = 2.235e-7
Identity = 28/57 (49.12%), Postives = 44/57 (77.19%), Query Frame = -1

Query:  626 MGCAISVEDKIAIERSKNIDKSLKEDGIQFXXXXXXXXXXXXXXXKSGKSTIFKQIK 796
            MGCA+S++++IA+ +SK+IDK LKEDG+Q     + + + ++G  +SGKSTI KQ+K
Sbjct:    1 MGCAMSLDERIALNKSKDIDKKLKEDGLQL---QRDIKLLLLGAGESGKSTIMKQMK 54

The following BLAST results are available for this feature:

BLAST of SMED30021189 vs. Ensembl Human
BLAST of SMED30021189 vs. Ensembl Celegans
BLAST of SMED30021189 vs. Ensembl Fly
BLAST of SMED30021189 vs. Ensembl Zebrafish
BLAST of SMED30021189 vs. Ensembl Xenopus
BLAST of SMED30021189 vs. Ensembl Mouse
BLAST of SMED30021189 vs. UniProt/SwissProt
BLAST of SMED30021189 vs. TrEMBL
BLAST of SMED30021189 vs. Ensembl Cavefish
BLAST of SMED30021189 vs. Ensembl Sea Lamprey
BLAST of SMED30021189 vs. Ensembl Yeast
BLAST of SMED30021189 vs. Ensembl Nematostella
BLAST of SMED30021189 vs. Ensembl Medaka
BLAST of SMED30021189 vs. Planmine SMEST

BLAST of SMED30021189 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
GNAO1	3.095e-8	48.53	G protein subunit alpha o1 [Source:HGNC Symbol;Acc...	[more]
GNAO1	9.080e-8	50.88	G protein subunit alpha o1 [Source:HGNC Symbol;Acc...	[more]
GNAO1	1.868e-7	50.00	G protein subunit alpha o1 [Source:HGNC Symbol;Acc...	[more]
GNAI1	1.978e-7	50.00	G protein subunit alpha i1 [Source:HGNC Symbol;Acc...	[more]
GNAO1	2.903e-7	50.00	G protein subunit alpha o1 [Source:HGNC Symbol;Acc...	[more]

BLAST of SMED30021189 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
goa-1	2.349e-6	48.28	Guanine nucleotide-binding protein G(o) subunit al...	[more]

BLAST of SMED30021189 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Galphao	4.039e-7	51.72	gene:FBgn0001122 transcript:FBtr0088267	[more]
Galphao	4.039e-7	51.72	gene:FBgn0001122 transcript:FBtr0088269	[more]
Galphao	4.039e-7	51.72	gene:FBgn0001122 transcript:FBtr0088264	[more]
Galphao	4.039e-7	51.72	gene:FBgn0001122 transcript:FBtr0088268	[more]
Galphao	4.039e-7	51.72	gene:FBgn0001122 transcript:FBtr0088270	[more]

BLAST of SMED30021189 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
gnai2b	1.397e-6	51.72	guanine nucleotide binding protein (G protein), al...	[more]
gnai2a	2.520e-6	51.72	guanine nucleotide binding protein (G protein), al...	[more]
gnai1	3.615e-6	45.45	guanine nucleotide binding protein (G protein), al...	[more]
gnai3	5.243e-6	48.28	guanine nucleotide binding protein (G protein), al...	[more]
gnao1a	5.387e-6	48.28	guanine nucleotide binding protein (G protein), al...	[more]

BLAST of SMED30021189 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
gnai2	1.626e-7	53.45	guanine nucleotide binding protein (G protein), al...	[more]
gnai2	1.895e-7	54.39	guanine nucleotide binding protein (G protein), al...	[more]
gnao1	1.526e-6	50.00	G protein subunit alpha o1 [Source:NCBI gene;Acc:5...	[more]
gnao1	1.597e-6	50.00	G protein subunit alpha o1 [Source:NCBI gene;Acc:5...	[more]
gnai1	1.639e-6	50.00	guanine nucleotide binding protein (G protein), al...	[more]

BLAST of SMED30021189 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Gnai3	1.254e-6	51.72	guanine nucleotide binding protein (G protein), al...	[more]
Gnai1	1.313e-6	50.00	guanine nucleotide binding protein (G protein), al...	[more]
Gnai2	1.454e-6	51.72	guanine nucleotide binding protein (G protein), al...	[more]
Gnai2	1.454e-6	51.72	guanine nucleotide binding protein (G protein), al...	[more]
Gnao1	1.820e-6	50.00	guanine nucleotide binding protein, alpha O [Sourc...	[more]

BLAST of SMED30021189 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|O15976\|GNAO_MIZYE	2.470e-6	51.72	Guanine nucleotide-binding protein G(o) subunit al...	[more]
sp\|P38404\|GNAO_LOCMI	5.467e-6	50.88	Guanine nucleotide-binding protein G(o) subunit al...	[more]
sp\|P30683\|GNAO_LYMST	6.324e-6	50.00	Guanine nucleotide-binding protein G(o) subunit al...	[more]
sp\|P51877\|GNAO_PLATR	6.440e-6	50.00	Guanine nucleotide-binding protein G(o) subunit al...	[more]
sp\|Q5RAD4\|GNAI1_PONAB	8.460e-6	50.00	Guanine nucleotide-binding protein G(i) subunit al...	[more]

BLAST of SMED30021189 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1


Match Name	E-value	Identity	Description
A0A2J8VYP2	4.003e-6	48.61	GNAO1 isoform 5 OS=Pongo abelii OX=9601 GN=CR201_G...	[more]

BLAST of SMED30021189 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000044204.1	1.641e-7	47.37	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
gnai2a	7.793e-7	53.45	guanine nucleotide-binding protein G(i) subunit al...	[more]
gnai2b	1.121e-6	51.72	guanine nucleotide-binding protein G(i) subunit al...	[more]
gnao1a	4.170e-6	48.28	G protein subunit alpha o1 [Source:NCBI gene;Acc:1...	[more]
gnao1a	4.607e-6	48.28	G protein subunit alpha o1 [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30021189 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gnai3	3.705e-7	48.28	guanine nucleotide binding protein (G protein), al...	[more]

BLAST of SMED30021189 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30021189 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO49692	2.949e-6	48.28	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30021189 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
gnai2b	1.065e-6	51.72	guanine nucleotide-binding protein G(i) subunit al...	[more]
ENSORLT00000020087.2	3.274e-6	45.45	guanine nucleotide-binding protein G(i) subunit al...	[more]
gnai3	3.617e-6	51.72	guanine nucleotide-binding protein G(k) subunit al...	[more]
gnai3	3.780e-6	51.72	guanine nucleotide-binding protein G(k) subunit al...	[more]
gnai2	6.651e-6	50.00	guanine nucleotide-binding protein G(i) subunit al...	[more]

BLAST of SMED30021189 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000081490.1	9.003e-16	82.76	SMESG000081490.1	[more]
SMESG000081490.1	7.136e-15	82.76	SMESG000081490.1	[more]
SMESG000035762.1	1.018e-10	64.58	SMESG000035762.1	[more]
SMESG000056187.1	2.087e-9	61.70	SMESG000056187.1	[more]
SMESG000081487.1	2.235e-7	49.12	SMESG000081487.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30021189 ID=SMED30021189|Name=SMED30021189|organism=Schmidtea mediterranea sexual|type=transcript|length=844bp
CACACAGACCTTCTAACGGTAGTGCGTTAAACCCACTATGAGATCGGACC
TTAGCAGTTCTTTCGTAGCGCTCACCTTTAGCGATCTCATAACCAGTGGT
TACATTACCAACGTCGCCTAGCAGTGGGTAACCATTGCGTGCGGTTGAAC
TTTGTATGTCTAGGGTTGAATCTTAAACTACGAACCCACTAATATTATAT
TTGATGGCACCTCAAAGAAGATATCCTTTGGTGATTATTCAAGAATCGCA
GTATAAAACAAAAAAGCCGCAATGCTGGCAAATTTAGCATGCCTGGTAAC
CACCCTAACTGGTGTGAGGCATCAATCGCCAGTTTATCTGTGTCTTCTAC
CCCGTCAACAACATCTCAGTCCACTGCGCCGATCATTGAACGAGTAGAAC
CTTCCGTGGTTTCCTAGAAAGCGGTCCCAAAATCTACAGTTTCTTCTTCT
CGCACCCGTCTCCATACATCAAATAGATAGCCCAATTACGATGTGGGTGG
ACGAAGTTGGGGAGGTGATCCATAATGTCATCCAAGTCCAATTCCCTCTT
CGACCACAGAAAAAGTGTAAATGTTTTCCCACGGACATTGACGGATAATG
GATAGCCCGGCGGGTTCTTGCGAAACTTTATTTGTTTAAAAATTGTACTC
TTCCCGGATTTTTCTGTACCTATAATTTCAATAATTAATAATTTGATTCC
TTTCAGGAATTGAATACCATCTTCTTTTAGTGATTTGTCTATATTTTTAC
TTCTTTCGATAGCAATTTTATCTTCAACAGAAATTGCACAACCCATTTTA
AAATAAAAGTAAAATGTAGTTTTATAAATATGATTCAAAAAATC

protein sequence of SMED30021189-orf-1

>SMED30021189-orf-1 ID=SMED30021189-orf-1|Name=SMED30021189-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=137bp
DFLNHIYKTTFYFYFKMGCAISVEDKIAIERSKNIDKSLKEDGIQFLKGI
KLLIIEIIGTEKSGKSTIFKQIKFRKNPPGYPLSVNVRGKTFTLFLWSKR
ELDLDDIMDHLPNFVHPHRNWAIYLMYGDGCEKKKL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000099	neuron
PLANA:0000101	muscle cell

Vocabulary: **INTERPRO**
Term	Definition
IPR027417	P-loop_NTPase

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SMED30021189

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

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