PRKCA-binding protein

Overview
NamePRKCA-binding protein
Smed IDSMED30021178
Length (bp)1795
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of PRKCA-binding protein (SMED30021178) t-SNE clustered cells

Violin plots show distribution of expression levels for PRKCA-binding protein (SMED30021178) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of PRKCA-binding protein (SMED30021178) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for PRKCA-binding protein (SMED30021178) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021178

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30021178h1SMcG0014286 Contig45042newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021178h1SMcG0014286 Contig45042uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021178h1SMcG0014286 Contig53820uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021178h1SMcG0014286 Contig53820newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30021178h1SMcG0014286 dd_Smed_v4_2968_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30021178h1SMcG0014286 dd_Smed_v4_2968_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021178h1SMcG0014286 dd_Smed_v4_2968_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30021178h1SMcG0014286 dd_Smed_v4_2968_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30021178h1SMcG0014286 dd_Smed_v4_2968_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Human
Match: PICK1 (protein interacting with PRKCA 1 [Source:HGNC Symbol;Acc:HGNC:9394])

HSP 1 Score: 331.257 bits (848), Expect = 1.252e-107
Identity = 152/225 (67.56%), Postives = 186/225 (82.67%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL + V  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 4.263e-66
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Human
Match: PICK1 (protein interacting with PRKCA 1 [Source:HGNC Symbol;Acc:HGNC:9394])

HSP 1 Score: 331.257 bits (848), Expect = 1.252e-107
Identity = 152/225 (67.56%), Postives = 186/225 (82.67%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL + V  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 4.263e-66
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Human
Match: PICK1 (protein interacting with PRKCA 1 [Source:HGNC Symbol;Acc:HGNC:9394])

HSP 1 Score: 224.557 bits (571), Expect = 5.343e-69
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146

HSP 2 Score: 108.612 bits (270), Expect = 3.821e-26
Identity = 48/83 (57.83%), Postives = 67/83 (80.72%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIK 909
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IK
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIK 229
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Human
Match: PICK1 (protein interacting with PRKCA 1 [Source:HGNC Symbol;Acc:HGNC:9394])

HSP 1 Score: 200.29 bits (508), Expect = 4.757e-61
Identity = 92/129 (71.32%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTA 481
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TA
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTA 134
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Human
Match: PICK1 (protein interacting with PRKCA 1 [Source:HGNC Symbol;Acc:HGNC:9394])

HSP 1 Score: 169.859 bits (429), Expect = 1.230e-49
Identity = 78/111 (70.27%), Postives = 95/111 (85.59%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDI 427
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDI 116
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Celegans
Match: Y57G11C.22 (pep chromosome:WBcel235:IV:14837112:14853662:1 gene:WBGene00013317.1 transcript:Y57G11C.22a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y57G11C.22)

HSP 1 Score: 216.083 bits (549), Expect = 1.521e-63
Identity = 102/196 (52.04%), Postives = 135/196 (68.88%), Query Frame = 1 
Query:  760 LSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSN 1347
            +S+  + FG +F  +   E Q  A+EAF+ FG+ HR + +   +    ++ M+SDL  ++   +PDTRLTIKKYLD K+EYLSYCLK+KEMDDEE    A+QEPLYRVETGNYEYR++LRCRQ+ R RF KMR+DV+VKIELLD KHV+DI   L  F K M+K  Q+C   +KE    PIE+DL +     + S+
Sbjct:  176 ISECQKEFGSIFCDLAAHEKQQTANEAFSSFGDKHRMIAKKQSESAVPLQKMVSDLQVYIDHVVPDTRLTIKKYLDVKYEYLSYCLKLKEMDDEEVEFVAIQEPLYRVETGNYEYRVMLRCRQECRARFMKMRDDVMVKIELLDQKHVRDIAQHLAIFAKTMAKCQQECAEILKERIDVPIEIDLEQLNLNMNTSD 371

HSP 2 Score: 141.354 bits (355), Expect = 2.701e-36
Identity = 86/181 (47.51%), Postives = 113/181 (62.43%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKNSLCIRNREFLLIL*LACAYINFGICF 637
            D+ +EED++GM + S ++ L KD + ++GISIGGG PYCPC+YVVQ+FD +PA KDG +  GDEI  +N  +VK + K  VA+LIQ     V I  NKL     +GKTLDI++KKVKH+VVE +   +ADA GLSRAIL N         ++ K  +   N EF   L    AY  FG  F
Sbjct:    2 DFQIEEDRLGMRIQSETIELTKDEKGVVGISIGGGGPYCPCVYVVQVFDKSPAFKDGRIRCGDEIVAINGITVKGERKSAVAQLIQVSLNPVKITINKLEEANTKGKTLDILIKKVKHKVVEFVDQDSADALGLSRAILTN-------DPLAEKEKILEENAEFYRHL---VAY--FGDMF 170
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Fly
Match: PICK1 (gene:FBgn0032447 transcript:FBtr0340542)

HSP 1 Score: 270.011 bits (689), Expect = 2.041e-83
Identity = 126/230 (54.78%), Postives = 172/230 (74.78%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKE-TDIFPIEVDLSRHTFTYDKSN 1347
            D LVKRL+E+  T  ++ GLVEH++ +++A YDL + Y++FG+ F+QI V EPQ RASEAF  FG  HR +E+  + ++K+IKP+++DL T+L KAIPDT+LT+++Y DAKF YLSYCLKVKEMDDEE+  +A+QEPLYRVETGNYEYRLILRCRQDAR +FAK+R DVL K+ELL+ KH  D+  QL   ++++++ H+  +  +     +FPIEVD     F Y  S 
Sbjct:  132 DSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVHEPQQRASEAFRTFGEFHRTLEKDGLGIIKQIKPVLADLGTYLNKAIPDTKLTVRRYADAKFTYLSYCLKVKEMDDEEHGFAALQEPLYRVETGNYEYRLILRCRQDARSKFAKLRTDVLEKMELLECKHAMDLNKQLRSLLESLAELHRSLVDRLDSLPPLFPIEVDFKETDFQYKSST 361

HSP 2 Score: 199.519 bits (506), Expect = 7.916e-57
Identity = 101/182 (55.49%), Postives = 127/182 (69.78%), Query Frame = 2 
Query:  125 MTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN----SLCIRNREFL-LIL*LACAYINFGICFY*LMTH 655
            MTV++ +V + KD  NLIGISIGGGAP CPCLY+VQIFD TPA+++GSL SGDE+  VN  SVK K+KVEVA++IQ+   EV I+YNKLHADP+QGKTLDIILKK+KHR+V+N++S+TAD  GLSRAILCN   ++    +         L    R  L     L   Y +FG CF  +  H
Sbjct:    1 MTVSTNAVVITKDQSNLIGISIGGGAPMCPCLYIVQIFDGTPAAREGSLQSGDELLAVNSVSVKGKTKVEVAKMIQTATDEVVIHYNKLHADPEQGKTLDIILKKLKHRIVDNLSSNTADTLGLSRAILCNDSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVH 182
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Fly
Match: PICK1 (gene:FBgn0032447 transcript:FBtr0340544)

HSP 1 Score: 270.011 bits (689), Expect = 2.041e-83
Identity = 126/230 (54.78%), Postives = 172/230 (74.78%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKE-TDIFPIEVDLSRHTFTYDKSN 1347
            D LVKRL+E+  T  ++ GLVEH++ +++A YDL + Y++FG+ F+QI V EPQ RASEAF  FG  HR +E+  + ++K+IKP+++DL T+L KAIPDT+LT+++Y DAKF YLSYCLKVKEMDDEE+  +A+QEPLYRVETGNYEYRLILRCRQDAR +FAK+R DVL K+ELL+ KH  D+  QL   ++++++ H+  +  +     +FPIEVD     F Y  S 
Sbjct:  132 DSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVHEPQQRASEAFRTFGEFHRTLEKDGLGIIKQIKPVLADLGTYLNKAIPDTKLTVRRYADAKFTYLSYCLKVKEMDDEEHGFAALQEPLYRVETGNYEYRLILRCRQDARSKFAKLRTDVLEKMELLECKHAMDLNKQLRSLLESLAELHRSLVDRLDSLPPLFPIEVDFKETDFQYKSST 361

HSP 2 Score: 199.519 bits (506), Expect = 7.916e-57
Identity = 101/182 (55.49%), Postives = 127/182 (69.78%), Query Frame = 2 
Query:  125 MTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN----SLCIRNREFL-LIL*LACAYINFGICFY*LMTH 655
            MTV++ +V + KD  NLIGISIGGGAP CPCLY+VQIFD TPA+++GSL SGDE+  VN  SVK K+KVEVA++IQ+   EV I+YNKLHADP+QGKTLDIILKK+KHR+V+N++S+TAD  GLSRAILCN   ++    +         L    R  L     L   Y +FG CF  +  H
Sbjct:    1 MTVSTNAVVITKDQSNLIGISIGGGAPMCPCLYIVQIFDGTPAAREGSLQSGDELLAVNSVSVKGKTKVEVAKMIQTATDEVVIHYNKLHADPEQGKTLDIILKKLKHRIVDNLSSNTADTLGLSRAILCNDSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVH 182
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Fly
Match: PICK1 (gene:FBgn0032447 transcript:FBtr0080396)

HSP 1 Score: 270.011 bits (689), Expect = 2.041e-83
Identity = 126/230 (54.78%), Postives = 172/230 (74.78%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKE-TDIFPIEVDLSRHTFTYDKSN 1347
            D LVKRL+E+  T  ++ GLVEH++ +++A YDL + Y++FG+ F+QI V EPQ RASEAF  FG  HR +E+  + ++K+IKP+++DL T+L KAIPDT+LT+++Y DAKF YLSYCLKVKEMDDEE+  +A+QEPLYRVETGNYEYRLILRCRQDAR +FAK+R DVL K+ELL+ KH  D+  QL   ++++++ H+  +  +     +FPIEVD     F Y  S 
Sbjct:  132 DSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVHEPQQRASEAFRTFGEFHRTLEKDGLGIIKQIKPVLADLGTYLNKAIPDTKLTVRRYADAKFTYLSYCLKVKEMDDEEHGFAALQEPLYRVETGNYEYRLILRCRQDARSKFAKLRTDVLEKMELLECKHAMDLNKQLRSLLESLAELHRSLVDRLDSLPPLFPIEVDFKETDFQYKSST 361

HSP 2 Score: 199.519 bits (506), Expect = 7.916e-57
Identity = 101/182 (55.49%), Postives = 127/182 (69.78%), Query Frame = 2 
Query:  125 MTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN----SLCIRNREFL-LIL*LACAYINFGICFY*LMTH 655
            MTV++ +V + KD  NLIGISIGGGAP CPCLY+VQIFD TPA+++GSL SGDE+  VN  SVK K+KVEVA++IQ+   EV I+YNKLHADP+QGKTLDIILKK+KHR+V+N++S+TAD  GLSRAILCN   ++    +         L    R  L     L   Y +FG CF  +  H
Sbjct:    1 MTVSTNAVVITKDQSNLIGISIGGGAPMCPCLYIVQIFDGTPAAREGSLQSGDELLAVNSVSVKGKTKVEVAKMIQTATDEVVIHYNKLHADPEQGKTLDIILKKLKHRIVDNLSSNTADTLGLSRAILCNDSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVH 182
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Fly
Match: PICK1 (gene:FBgn0032447 transcript:FBtr0080395)

HSP 1 Score: 270.011 bits (689), Expect = 3.619e-83
Identity = 126/230 (54.78%), Postives = 172/230 (74.78%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKE-TDIFPIEVDLSRHTFTYDKSN 1347
            D LVKRL+E+  T  ++ GLVEH++ +++A YDL + Y++FG+ F+QI V EPQ RASEAF  FG  HR +E+  + ++K+IKP+++DL T+L KAIPDT+LT+++Y DAKF YLSYCLKVKEMDDEE+  +A+QEPLYRVETGNYEYRLILRCRQDAR +FAK+R DVL K+ELL+ KH  D+  QL   ++++++ H+  +  +     +FPIEVD     F Y  S 
Sbjct:  149 DSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVHEPQQRASEAFRTFGEFHRTLEKDGLGIIKQIKPVLADLGTYLNKAIPDTKLTVRRYADAKFTYLSYCLKVKEMDDEEHGFAALQEPLYRVETGNYEYRLILRCRQDARSKFAKLRTDVLEKMELLECKHAMDLNKQLRSLLESLAELHRSLVDRLDSLPPLFPIEVDFKETDFQYKSST 378

HSP 2 Score: 217.624 bits (553), Expect = 2.005e-63
Identity = 110/196 (56.12%), Postives = 137/196 (69.90%), Query Frame = 2 
Query:   83 DLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN----SLCIRNREFL-LIL*LACAYINFGICFY*LMTH 655
            D  DD+  EEDKMGMTV++ +V + KD  NLIGISIGGGAP CPCLY+VQIFD TPA+++GSL SGDE+  VN  SVK K+KVEVA++IQ+   EV I+YNKLHADP+QGKTLDIILKK+KHR+V+N++S+TAD  GLSRAILCN   ++    +         L    R  L     L   Y +FG CF  +  H
Sbjct:    4 DTEDDFFFEEDKMGMTVSTNAVVITKDQSNLIGISIGGGAPMCPCLYIVQIFDGTPAAREGSLQSGDELLAVNSVSVKGKTKVEVAKMIQTATDEVVIHYNKLHADPEQGKTLDIILKKLKHRIVDNLSSNTADTLGLSRAILCNDSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVH 199
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Fly
Match: PICK1 (gene:FBgn0032447 transcript:FBtr0340543)

HSP 1 Score: 270.011 bits (689), Expect = 2.120e-82
Identity = 126/230 (54.78%), Postives = 172/230 (74.78%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKE-TDIFPIEVDLSRHTFTYDKSN 1347
            D LVKRL+E+  T  ++ GLVEH++ +++A YDL + Y++FG+ F+QI V EPQ RASEAF  FG  HR +E+  + ++K+IKP+++DL T+L KAIPDT+LT+++Y DAKF YLSYCLKVKEMDDEE+  +A+QEPLYRVETGNYEYRLILRCRQDAR +FAK+R DVL K+ELL+ KH  D+  QL   ++++++ H+  +  +     +FPIEVD     F Y  S 
Sbjct:  222 DSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVHEPQQRASEAFRTFGEFHRTLEKDGLGIIKQIKPVLADLGTYLNKAIPDTKLTVRRYADAKFTYLSYCLKVKEMDDEEHGFAALQEPLYRVETGNYEYRLILRCRQDARSKFAKLRTDVLEKMELLECKHAMDLNKQLRSLLESLAELHRSLVDRLDSLPPLFPIEVDFKETDFQYKSST 451

HSP 2 Score: 206.453 bits (524), Expect = 1.410e-58
Identity = 104/188 (55.32%), Postives = 132/188 (70.21%), Query Frame = 2 
Query:  107 EEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN----SLCIRNREFL-LIL*LACAYINFGICFY*LMTH 655
            E D++GMTV++ +V + KD  NLIGISIGGGAP CPCLY+VQIFD TPA+++GSL SGDE+  VN  SVK K+KVEVA++IQ+   EV I+YNKLHADP+QGKTLDIILKK+KHR+V+N++S+TAD  GLSRAILCN   ++    +         L    R  L     L   Y +FG CF  +  H
Sbjct:   85 ELDRLGMTVSTNAVVITKDQSNLIGISIGGGAPMCPCLYIVQIFDGTPAAREGSLQSGDELLAVNSVSVKGKTKVEVAKMIQTATDEVVIHYNKLHADPEQGKTLDIILKKLKHRIVDNLSSNTADTLGLSRAILCNDSLVKRLEELEGTELMYKGLVEHARRMLKAYYDLLQTYKSFGDCFTQISVH 272
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Zebrafish
Match: pick1 (protein interacting with prkca 1 [Source:ZFIN;Acc:ZDB-GENE-061013-283])

HSP 1 Score: 334.339 bits (856), Expect = 4.195e-109
Identity = 156/225 (69.33%), Postives = 184/225 (81.78%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ KT  L+ GL+EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQ  ASEAF  F  AHRNME++ + +LK IKPM+ DLNT+L KAIPDT+LTI+KYLD KFEYLSYCLKVKEMDDEEYS  A+ +PLYRV TGNYEYRL+LRCRQ+AR RFAKMR DVL KIELLD KHVQDIV QL +FV  MS Y+ DC A +K+ D+FPIEVDLSR    Y
Sbjct:  147 DGLVKRLEELEKTAELYKGLMEHTKRLLRAFYELSQTHRAFGDVFSVIGVREPQAAASEAFVKFAEAHRNMEKFGIQLLKTIKPMLHDLNTYLHKAIPDTKLTIRKYLDVKFEYLSYCLKVKEMDDEEYSCIALGDPLYRVSTGNYEYRLVLRCRQEARARFAKMRKDVLEKIELLDQKHVQDIVFQLQRFVSGMSHYYDDCYAVLKDADVFPIEVDLSRTMINY 371

HSP 2 Score: 219.935 bits (559), Expect = 3.920e-65
Identity = 102/141 (72.34%), Postives = 122/141 (86.52%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY LEEDK+G+    G+VTL KD+QNLIGISIGGGA +CPCLY+VQ+FDNT A+ DG+L +GDEITGVN + VK K+KVEVA++IQ+ +GEV I YNKL ADPKQGK+LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYELEEDKLGIPTVPGTVTLKKDSQNLIGISIGGGAQFCPCLYIVQVFDNTAAALDGTLAAGDEITGVNGKPVKGKTKVEVAKMIQAVQGEVVIQYNKLQADPKQGKSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Zebrafish
Match: fhdc3 (FH2 domain containing 3 [Source:ZFIN;Acc:ZDB-GENE-061103-76])

HSP 1 Score: 61.6178 bits (148), Expect = 9.635e-10
Identity = 47/192 (24.48%), Postives = 92/192 (47.92%), Query Frame = 1 
Query:  730 SKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY----HQDCLATMKETDI 1293
            ++ L    Y + +   A G+ F+ +  K P+ R  + F       + + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E++     A  M     R+E    +Y++        + ++ ++R+DV +KI+ L+   V+ +  QL  F  A+S Y     Q    T+K+ +I
Sbjct:  150 ARALTNHFYSMVQTQHALGDTFADLSQKSPELR--DEFGYNAETQKLLCKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKMYENARLEFDAYRADLEELNMGPRDAVTMA----RIEAAQQQYQI-------HKDKYERLRSDVTIKIKFLEENKVKVMHKQLLLFHNAISAYFAGNQQQLEQTLKQFNI 328
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Zebrafish
Match: arfip2b (ADP-ribosylation factor interacting protein 2b [Source:ZFIN;Acc:ZDB-GENE-050417-131])

HSP 1 Score: 61.2326 bits (147), Expect = 1.302e-9
Identity = 47/192 (24.48%), Postives = 92/192 (47.92%), Query Frame = 1 
Query:  730 SKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY----HQDCLATMKETDI 1293
            ++ L    Y + +   A G+ F+ +  K P+ R  + F       + + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E++     A  M     R+E    +Y++        + ++ ++R+DV +KI+ L+   V+ +  QL  F  A+S Y     Q    T+K+ +I
Sbjct:  161 ARALTNHFYSMVQTQHALGDTFADLSQKSPELR--DEFGYNAETQKLLCKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKMYENARLEFDAYRADLEELNMGPRDAVTMA----RIEAAQQQYQI-------HKDKYERLRSDVTIKIKFLEENKVKVMHKQLLLFHNAISAYFAGNQQQLEQTLKQFNI 339
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Zebrafish
Match: arfip2b (ADP-ribosylation factor interacting protein 2b [Source:ZFIN;Acc:ZDB-GENE-050417-131])

HSP 1 Score: 61.2326 bits (147), Expect = 1.302e-9
Identity = 47/192 (24.48%), Postives = 92/192 (47.92%), Query Frame = 1 
Query:  730 SKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY----HQDCLATMKETDI 1293
            ++ L    Y + +   A G+ F+ +  K P+ R  + F       + + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E++     A  M     R+E    +Y++        + ++ ++R+DV +KI+ L+   V+ +  QL  F  A+S Y     Q    T+K+ +I
Sbjct:  161 ARALTNHFYSMVQTQHALGDTFADLSQKSPELR--DEFGYNAETQKLLCKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKMYENARLEFDAYRADLEELNMGPRDAVTMA----RIEAAQQQYQI-------HKDKYERLRSDVTIKIKFLEENKVKVMHKQLLLFHNAISAYFAGNQQQLEQTLKQFNI 339
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Zebrafish
Match: arfip2b (ADP-ribosylation factor interacting protein 2b [Source:ZFIN;Acc:ZDB-GENE-050417-131])

HSP 1 Score: 61.2326 bits (147), Expect = 1.302e-9
Identity = 47/192 (24.48%), Postives = 92/192 (47.92%), Query Frame = 1 
Query:  730 SKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY----HQDCLATMKETDI 1293
            ++ L    Y + +   A G+ F+ +  K P+ R  + F       + + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E++     A  M     R+E    +Y++        + ++ ++R+DV +KI+ L+   V+ +  QL  F  A+S Y     Q    T+K+ +I
Sbjct:  161 ARALTNHFYSMVQTQHALGDTFADLSQKSPELR--DEFGYNAETQKLLCKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKMYENARLEFDAYRADLEELNMGPRDAVTMA----RIEAAQQQYQI-------HKDKYERLRSDVTIKIKFLEENKVKVMHKQLLLFHNAISAYFAGNQQQLEQTLKQFNI 339
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Xenopus
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:734086])

HSP 1 Score: 338.576 bits (867), Expect = 2.088e-110
Identity = 156/231 (67.53%), Postives = 191/231 (82.68%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQF 1353
            DGLVKRL+E+ KTG  + G++EH+K L+R+ ++LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM+SDLNT+L KAIPDT+LTI+KYLD KFEYLSYCLKVKEMDDEEYS+ A+ EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHV DIV QL +FV AMSKY+  C   +KE DIFPIEVDL++ T  Y + + F
Sbjct:  163 DGLVKRLEELEKTGEFYRGMMEHTKRLLRSFFELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIGLLKTIKPMLSDLNTYLNKAIPDTKLTIRKYLDVKFEYLSYCLKVKEMDDEEYSSIALGEPLYRVGTGNYEYRLILRCRQEARARFAKMRKDVLEKIELLDQKHVHDIVFQLQRFVSAMSKYNDQCYEVLKEADIFPIEVDLAKTTLAYGQGDTF 393

HSP 2 Score: 208.379 bits (529), Expect = 1.718e-60
Identity = 96/132 (72.73%), Postives = 118/132 (89.39%), Query Frame = 2 
Query:  122 GMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            G+    G+VTL KD+QN+IGISIGGGA +CPCLY+VQ+FDNTPAS DG++ +GDEITGVN +SVK K+KVEVA++IQ+ +GEVTI+YNKL ADPKQGK+LDI+LKKVKHR+VEN++S TADA GLSRAILCN
Sbjct:   31 GIPTVPGTVTLKKDSQNVIGISIGGGAQHCPCLYIVQVFDNTPASLDGTIAAGDEITGVNGKSVKGKTKVEVAKMIQNVKGEVTIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSSGTADALGLSRAILCN 162
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Xenopus
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:734086])

HSP 1 Score: 337.806 bits (865), Expect = 2.342e-110
Identity = 156/231 (67.53%), Postives = 191/231 (82.68%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQF 1353
            DGLVKRL+E+ KTG  + G++EH+K L+R+ ++LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM+SDLNT+L KAIPDT+LTI+KYLD KFEYLSYCLKVKEMDDEEYS+ A+ EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHV DIV QL +FV AMSKY+  C   +KE DIFPIEVDL++ T  Y + + F
Sbjct:  144 DGLVKRLEELEKTGEFYRGMMEHTKRLLRSFFELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIGLLKTIKPMLSDLNTYLNKAIPDTKLTIRKYLDVKFEYLSYCLKVKEMDDEEYSSIALGEPLYRVGTGNYEYRLILRCRQEARARFAKMRKDVLEKIELLDQKHVHDIVFQLQRFVSAMSKYNDQCYEVLKEADIFPIEVDLAKTTLAYGQGDTF 374

HSP 2 Score: 208.379 bits (529), Expect = 9.251e-61
Identity = 97/140 (69.29%), Postives = 120/140 (85.71%), Query Frame = 2 
Query:   98 YPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            +P      G+    G+VTL KD+QN+IGISIGGGA +CPCLY+VQ+FDNTPAS DG++ +GDEITGVN +SVK K+KVEVA++IQ+ +GEVTI+YNKL ADPKQGK+LDI+LKKVKHR+VEN++S TADA GLSRAILCN
Sbjct:    4 HPFWLSVYGIPTVPGTVTLKKDSQNVIGISIGGGAQHCPCLYIVQVFDNTPASLDGTIAAGDEITGVNGKSVKGKTKVEVAKMIQNVKGEVTIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSSGTADALGLSRAILCN 143
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Xenopus
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:734086])

HSP 1 Score: 337.421 bits (864), Expect = 4.053e-110
Identity = 156/231 (67.53%), Postives = 191/231 (82.68%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQF 1353
            DGLVKRL+E+ KTG  + G++EH+K L+R+ ++LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM+SDLNT+L KAIPDT+LTI+KYLD KFEYLSYCLKVKEMDDEEYS+ A+ EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHV DIV QL +FV AMSKY+  C   +KE DIFPIEVDL++ T  Y + + F
Sbjct:  147 DGLVKRLEELEKTGEFYRGMMEHTKRLLRSFFELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIGLLKTIKPMLSDLNTYLNKAIPDTKLTIRKYLDVKFEYLSYCLKVKEMDDEEYSSIALGEPLYRVGTGNYEYRLILRCRQEARARFAKMRKDVLEKIELLDQKHVHDIVFQLQRFVSAMSKYNDQCYEVLKEADIFPIEVDLAKTTLAYGQGDTF 377

HSP 2 Score: 221.861 bits (564), Expect = 8.180e-66
Identity = 102/141 (72.34%), Postives = 125/141 (88.65%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY  EEDK+G+    G+VTL KD+QN+IGISIGGGA +CPCLY+VQ+FDNTPAS DG++ +GDEITGVN +SVK K+KVEVA++IQ+ +GEVTI+YNKL ADPKQGK+LDI+LKKVKHR+VEN++S TADA GLSRAILCN
Sbjct:    6 DYDFEEDKLGIPTVPGTVTLKKDSQNVIGISIGGGAQHCPCLYIVQVFDNTPASLDGTIAAGDEITGVNGKSVKGKTKVEVAKMIQNVKGEVTIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Xenopus
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:734086])

HSP 1 Score: 237.269 bits (604), Expect = 4.475e-72
Identity = 119/231 (51.52%), Postives = 152/231 (65.80%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQF 1353
            DGLVKRL+E+ KTG  + G++EH+K L+R+ ++LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKP                    +    +   Y S  L+            A+ EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHV DIV QL +FV AMSKY+  C   +KE DIFPIEVDL++ T  Y + + F
Sbjct:  140 DGLVKRLEELEKTGEFYRGMMEHTKRLLRSFFELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIGLLKTIKPE-------------------RNITSSPLMYFSVFLQ------------ALGEPLYRVGTGNYEYRLILRCRQEARARFAKMRKDVLEKIELLDQKHVHDIVFQLQRFVSAMSKYNDQCYEVLKEADIFPIEVDLAKTTLAYGQGDTF 339

HSP 2 Score: 208.379 bits (529), Expect = 4.151e-61
Identity = 96/132 (72.73%), Postives = 118/132 (89.39%), Query Frame = 2 
Query:  122 GMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            G+    G+VTL KD+QN+IGISIGGGA +CPCLY+VQ+FDNTPAS DG++ +GDEITGVN +SVK K+KVEVA++IQ+ +GEVTI+YNKL ADPKQGK+LDI+LKKVKHR+VEN++S TADA GLSRAILCN
Sbjct:    8 GIPTVPGTVTLKKDSQNVIGISIGGGAQHCPCLYIVQVFDNTPASLDGTIAAGDEITGVNGKSVKGKTKVEVAKMIQNVKGEVTIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSSGTADALGLSRAILCN 139
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Xenopus
Match: ARFIP2 (ADP ribosylation factor interacting protein 2 [Source:NCBI gene;Acc:100380075])

HSP 1 Score: 63.929 bits (154), Expect = 1.973e-10
Identity = 41/163 (25.15%), Postives = 77/163 (47.24%), Query Frame = 1 
Query:  715 GLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHV 1203
            G+++ ++ L    Y L +  RA G+ FS +  K P+    E F       + + +    +L  +   +S +NT + K + DT +T+K+Y  A+ E+ +Y   ++E+      A+     L R+ET    +       Q  R+++ K+R DV +K+  L    V
Sbjct:  127 GVLQLARALTAHFYSLVQTQRALGDAFSDLSQKSPE--LQEEFGYNAETQKLLCKNGETLLGAVNFFVSSINTLINKTMEDTLMTVKQYETARLEFDAYRADLEELSLGPRDAAT----LCRMETAQQNF-------QSHRVKYEKLRGDVTIKLRFLQENKV 276
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Mouse
Match: Pick1 (protein interacting with C kinase 1 [Source:MGI Symbol;Acc:MGI:894645])

HSP 1 Score: 333.569 bits (854), Expect = 1.279e-108
Identity = 153/225 (68.00%), Postives = 187/225 (83.11%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL +FV  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRFVSTMSKYYNDCYAVLQDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 3.005e-66
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGKSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Mouse
Match: Pick1 (protein interacting with C kinase 1 [Source:MGI Symbol;Acc:MGI:894645])

HSP 1 Score: 333.569 bits (854), Expect = 1.279e-108
Identity = 153/225 (68.00%), Postives = 187/225 (83.11%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL +FV  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRFVSTMSKYYNDCYAVLQDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 3.005e-66
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGKSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Mouse
Match: Pick1 (protein interacting with C kinase 1 [Source:MGI Symbol;Acc:MGI:894645])

HSP 1 Score: 333.569 bits (854), Expect = 1.279e-108
Identity = 153/225 (68.00%), Postives = 187/225 (83.11%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL +FV  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRFVSTMSKYYNDCYAVLQDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 3.005e-66
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGKSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Mouse
Match: Gm49486 (predicted gene, 49486 [Source:MGI Symbol;Acc:MGI:6155161])

HSP 1 Score: 276.174 bits (705), Expect = 4.885e-85
Identity = 127/180 (70.56%), Postives = 154/180 (85.56%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKH 1200
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KH
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKH 326

HSP 2 Score: 222.246 bits (565), Expect = 1.095e-64
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGKSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Mouse
Match: Arfip2 (ADP-ribosylation factor interacting protein 2 [Source:MGI Symbol;Acc:MGI:1924182])

HSP 1 Score: 60.077 bits (144), Expect = 2.738e-9
Identity = 43/179 (24.02%), Postives = 84/179 (46.93%), Query Frame = 1 
Query:  718 LVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY 1254
            +++  + L   +Y L +   A G+ F+ +  K P+    E F       + + +    +L  +   +S +NT +TK + DT +T+K+Y  A+ EY +Y   ++E+      A        R+E+    +       Q  R ++ K+R DV +K++ L+   ++ +  QL  F  A+S Y
Sbjct:  143 VLQLGRALTAHLYSLLQTQHALGDAFADLSQKSPE--LQEEFGYNAETQKLLCKNGETLLGAVNFFVSSINTLVTKTMEDTLMTVKQYEAARLEYDAYRTDLEELSLGPRDAGTRG----RLESAQATF-------QTHRDKYEKLRGDVAIKLKFLEENKIKVMHKQLLLFHNAVSAY 308
[Close]
BLAST of PRKCA-binding protein vs. UniProt/SwissProt
Match: sp|Q9EP80|PICK1_RAT (PRKCA-binding protein OS=Rattus norvegicus OX=10116 GN=Pick1 PE=1 SV=1)

HSP 1 Score: 333.954 bits (855), Expect = 7.140e-108
Identity = 153/225 (68.00%), Postives = 187/225 (83.11%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL +FV  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRFVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.402 bits (568), Expect = 1.778e-65
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. UniProt/SwissProt
Match: sp|Q2T9M1|PICK1_BOVIN (PRKCA-binding protein OS=Bos taurus OX=9913 GN=PICK1 PE=2 SV=1)

HSP 1 Score: 332.413 bits (851), Expect = 2.539e-107
Identity = 152/229 (66.38%), Postives = 188/229 (82.10%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSN 1347
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD +HVQDIV QL +FV  MSKY+ DC + +++ D+FPIEVDL+  T  Y  S 
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQEHVQDIVLQLQRFVSTMSKYYNDCYSVLRDADVFPIEVDLAHTTLAYGLSQ 375

HSP 2 Score: 221.09 bits (562), Expect = 1.464e-64
Identity = 102/140 (72.86%), Postives = 122/140 (87.14%), Query Frame = 2 
Query:   98 YPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            Y +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    7 YDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. UniProt/SwissProt
Match: sp|Q5REH1|PICK1_PONAB (PRKCA-binding protein OS=Pongo abelii OX=9601 GN=PICK1 PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 5.575e-107
Identity = 152/225 (67.56%), Postives = 186/225 (82.67%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL + V  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 2.026e-65
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. UniProt/SwissProt
Match: sp|Q9NRD5|PICK1_HUMAN (PRKCA-binding protein OS=Homo sapiens OX=9606 GN=PICK1 PE=1 SV=2)

HSP 1 Score: 331.257 bits (848), Expect = 6.011e-107
Identity = 152/225 (67.56%), Postives = 186/225 (82.67%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+RA Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL + V  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 2.047e-65
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. UniProt/SwissProt
Match: sp|Q4R7Q5|PICK1_MACFA (PRKCA-binding protein OS=Macaca fascicularis OX=9541 GN=PICK1 PE=2 SV=1)

HSP 1 Score: 329.717 bits (844), Expect = 2.651e-106
Identity = 151/225 (67.11%), Postives = 185/225 (82.22%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            DGLVKRL+E+ +T  L+ G+ EH+K L+R  Y+LS+ +RAFG+VFS IGV+EPQP ASEAF  F +AHR++E++ + +LK IKPM++DLNT+L KAIPDTRLTIKKYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RF++MR DVL K+ELLD KHVQDIV QL + V  MSKY+ DC A +++ D+FPIEVDL+  T  Y
Sbjct:  147 DGLVKRLEELERTAELYKGMTEHTKNLLRVFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAY 371

HSP 2 Score: 223.016 bits (567), Expect = 2.135e-65
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY +EEDK+G+    G VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG++ +GDEITGVN +S+K K+KVEVA++IQ  +GEVTI+YNKL ADPKQG +LDI+LKKVKHR+VENM+S TADA GLSRAILCN
Sbjct:    6 DYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCN 146
[Close]
BLAST of PRKCA-binding protein vs. TrEMBL
Match: U6JA56 (PRKCA-binding protein OS=Echinococcus granulosus OX=6210 GN=EGR_01970 PE=4 SV=1)

HSP 1 Score: 354.755 bits (909), Expect = 1.880e-157
Identity = 179/312 (57.37%), Postives = 238/312 (76.28%), Query Frame = 1 
Query:  637 LLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFXXXXXXXXXXXXXXXXXNKPNKN-DTVKNVVEKDSDL----LKDFGDSFADLNFSSNENQNPNTKQGETVDLL 1557
            +L+ND+    LVK++DE+N+T  +F GLV+H++ LVRAI+ LSKV+R FGE+ SQIGVKEPQ RA +AFT FG AHR +E++A+D LK+++P++SDLNTFLTKAIPDT+LT+K+YLD KFEYLSYCLKVKEMDDEEY+ +A+QEPLYRVETGNYEYRLILRCRQDAR RFAK+R+DVLVK+ELLDNKHVQD V QL +FV AM+ YH++C   MK   IFP+E+DLSR  F Y KS   +  + EDD+  D  +  N  + + + + ++V  +S+L    L +  D+  DL  S+ +   P+ +Q +  DL+
Sbjct:  141 ILVNDS----LVKKMDELNRTASMFNGLVQHTRSLVRAIFQLSKVHRTFGEIMSQIGVKEPQVRAGQAFTHFGEAHRELEKFAIDTLKQLQPIVSDLNTFLTKAIPDTKLTVKRYLDVKFEYLSYCLKVKEMDDEEYTFNALQEPLYRVETGNYEYRLILRCRQDARSRFAKLRSDVLVKLELLDNKHVQDTVIQLQRFVSAMATYHENCYEVMKGATIFPLEMDLSRDAFAY-KSELINTGEIEDDDDDDEIDVSNDNDVDVEALADLVSTESNLVNVSLAENVDTLLDLGESAEKTPKPHVEQKDVFDLI 447

HSP 2 Score: 232.646 bits (592), Expect = 1.880e-157
Identity = 108/144 (75.00%), Postives = 124/144 (86.11%), Query Frame = 2 
Query:   86 LYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            +YD Y  EEDK+GM VTSGS+TL KD QNL+GISIGGGAPYCPCLYVVQ+FDNTPAS+DG++ +GDEITGV    VK K KVEVARLIQS+   VTI +NKLHADPKQGKTLDI+LKK+KH++VENM +STADA GLSRAIL N
Sbjct:    1 MYDSYVFEEDKLGMMVTSGSITLKKDPQNLVGISIGGGAPYCPCLYVVQVFDNTPASRDGTIQAGDEITGVGGVRVKGKGKVEVARLIQSFPDSVTIYFNKLHADPKQGKTLDIVLKKLKHKMVENMEASTADALGLSRAILVN 144
[Close]
BLAST of PRKCA-binding protein vs. TrEMBL
Match: A0A158R7N2 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS4093 PE=4 SV=1)

HSP 1 Score: 351.673 bits (901), Expect = 6.444e-157
Identity = 159/233 (68.24%), Postives = 199/233 (85.41%), Query Frame = 1 
Query:  637 LLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            +L+ND+    L+K++DE+N+T  +F GLV+H++ LVRAI+ LSKV+R FGE+ SQIGVKEPQ RA +AFT FG AHR +E++A+D LK+++P++SDLNTFLTKAIPDT+LT+K+YLD KFEYLSYCLKVKEMDDEEY+ +A+QEPLYRVETGNYEYRLILRCRQDAR RFAK+RNDVL+K+ELLDNKHVQD V QL +FV AM+ YH++C   MK   IFP+E+DLSR  F Y
Sbjct:  141 ILVNDS----LLKKMDELNRTASMFNGLVQHTRSLVRAIFQLSKVHRTFGEIMSQIGVKEPQVRAGQAFTHFGEAHRELEKFAIDTLKQLRPIVSDLNTFLTKAIPDTKLTVKRYLDVKFEYLSYCLKVKEMDDEEYTFNALQEPLYRVETGNYEYRLILRCRQDARSRFAKLRNDVLIKLELLDNKHVQDTVIQLQRFVSAMATYHENCYEVMKGATIFPLEMDLSRDAFAY 369

HSP 2 Score: 234.187 bits (596), Expect = 6.444e-157
Identity = 109/144 (75.69%), Postives = 125/144 (86.81%), Query Frame = 2 
Query:   86 LYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            +YD Y  EEDK+GM VTSGSVTL KD QNL+GISIGGGAPYCPCLYVVQ+FDNTPAS+DG++ +GDEITGV    VK K KVEVARLIQS+   VTIN+NKLHADP+QGKTLDI+LKK+KH++VENM +STADA GLSRAIL N
Sbjct:    1 MYDSYVFEEDKLGMMVTSGSVTLKKDPQNLVGISIGGGAPYCPCLYVVQVFDNTPASRDGTIQAGDEITGVGGVRVKGKGKVEVARLIQSFPDSVTINFNKLHADPRQGKTLDIVLKKLKHKMVENMEASTADALGLSRAILVN 144
[Close]
BLAST of PRKCA-binding protein vs. TrEMBL
Match: R7VGQ0 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_197664 PE=4 SV=1)

HSP 1 Score: 357.836 bits (917), Expect = 1.409e-156
Identity = 167/245 (68.16%), Postives = 203/245 (82.86%), Query Frame = 1 
Query:  619 KFWDLFLLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQF 1353
            +FW  F        DGLVK+L+E+ KT  ++ GL +H+K L++A ++LS+ +RAFG+VF+ IGV+EPQP ASEAFT FG AHR +E+YA+  LK +KPMI DL+T+L+KA+PDTRLTI+KYLDAKFEYLSYCLKVKEMDDEEYS +A+QEPLYRVETGNYEYRL+LRCRQDAR RFAKMR+DVLVK+ELLDNKHVQDIV QL + V AM+KYH D  A +K  ++FPIEVDLSR TFTYD S QF
Sbjct:  196 EFWFCFCT------DGLVKKLEELEKTAQMYQGLSDHTKRLLKAFFELSQAHRAFGDVFAGIGVREPQPAASEAFTQFGEAHREIEKYAIQTLKTVKPMIHDLHTYLSKAVPDTRLTIRKYLDAKFEYLSYCLKVKEMDDEEYSYAALQEPLYRVETGNYEYRLVLRCRQDARTRFAKMRSDVLVKMELLDNKHVQDIVFQLQRLVTAMAKYHNDSHAVLKVAEVFPIEVDLSRSTFTYDTSTQF 434

HSP 2 Score: 226.868 bits (577), Expect = 1.409e-156
Identity = 110/162 (67.90%), Postives = 127/162 (78.40%), Query Frame = 2 
Query:  110 EDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYR--------GEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKNSLCI 571
            ED MGM VTSGSVTL KD QNLIGISIGGGAPYCPCLYVVQIFDNTPA+K+G+L SGDEI GVN+ SVK ++KVEVAR IQ+ +        GEV INYNKLHA+PK GK+LDI+LKK+KHRVVE M +STADA GLSRAILCN +++   S    +   C 
Sbjct:   40 EDLMGMRVTSGSVTLEKDPQNLIGISIGGGAPYCPCLYVVQIFDNTPAAKEGTLASGDEIVGVNRTSVKGRTKVEVARQIQAVKVAIATVAVGEVVINYNKLHAEPKDGKSLDIVLKKMKHRVVETMNTSTADALGLSRAILCNGMALYILSKFDKEFWFCF 201
[Close]
BLAST of PRKCA-binding protein vs. TrEMBL
Match: A0A0X3P4P2 (PRKCA-binding protein OS=Schistocephalus solidus OX=70667 GN=PICK1 PE=4 SV=1)

HSP 1 Score: 348.206 bits (892), Expect = 5.074e-156
Identity = 157/233 (67.38%), Postives = 199/233 (85.41%), Query Frame = 1 
Query:  637 LLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTY 1335
            +L+ND    GLVK++DE+++T  +F GLV HS+ LVRAI++LSKV+  FG++ S+IGVKEPQ RA  AFT F +AHR+ME++A++ LKK++PM++DLNTFLTKAIPDT+LT+K+YLD KFEYLSYCLKVKEMDDEEY+ +A+QEPLYRVETGNYEYRL+LRCRQDAR+RFAK+RNDVLVK+ELLDNKHVQD V QL +FV A++ YH++C   MK   IFP+E+DLSR  F Y
Sbjct:  143 ILVND----GLVKKMDELDRTASMFAGLVTHSRSLVRAIFNLSKVHHDFGQILSEIGVKEPQMRAGLAFTQFADAHRDMEKFAIETLKKLRPMVADLNTFLTKAIPDTKLTVKRYLDVKFEYLSYCLKVKEMDDEEYTFNALQEPLYRVETGNYEYRLVLRCRQDARVRFAKLRNDVLVKLELLDNKHVQDTVLQLQRFVTALANYHENCYEVMKSAVIFPLEMDLSRDAFAY 371

HSP 2 Score: 234.572 bits (597), Expect = 5.074e-156
Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2 
Query:   80 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            M +YD Y  EEDKMGMTVTSGSVTL KD QNL+GISIGGGAPYCPCLYVVQ+FD+TPA++DG++ +GDEITGV   SVK K KVEVA LIQS++  VTI +NKLHADPKQGKTLDI+LKK+KHR+VENM +STADA GLSRAIL N
Sbjct:    1 MSVYDSYMYEEDKMGMTVTSGSVTLKKDPQNLVGISIGGGAPYCPCLYVVQVFDSTPAARDGTIQAGDEITGVGGISVKGKGKVEVAHLIQSFKETVTIFFNKLHADPKQGKTLDIVLKKMKHRMVENMEASTADALGLSRAILVN 146
[Close]
BLAST of PRKCA-binding protein vs. TrEMBL
Match: A0A2J7Q1Z0 (PRKCA-binding protein OS=Cryptotermes secundus OX=105785 GN=PICK1_4 PE=4 SV=1)

HSP 1 Score: 324.709 bits (831), Expect = 8.634e-150
Identity = 147/237 (62.03%), Postives = 193/237 (81.43%), Query Frame = 1 
Query:  637 LLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSN 1347
            +L NDT    LVK+L+E+ +T  ++ GLVEH+K +++A +DL +VY+AFG+ F++IGV+EPQPRASE F  FG+ HR ME+Y + MLK +KP++SDL T+L KAIPDT+LTI+KY D KFEYLSYCLKVKEMDDEEYS  A+QEPLYRVETGNYEYRLILRCRQDAR RFA++R+DVLVK+ELLDNKHVQD+V QL + V  ++++H + L+ ++   +FPIEVDL+R  F Y  ++
Sbjct:  144 ILCNDT----LVKKLEELEQTEMMYQGLVEHAKRVLKAFFDLLQVYKAFGDTFAEIGVREPQPRASEVFRQFGDYHRQMEKYGIKMLKSVKPILSDLGTYLYKAIPDTKLTIRKYADTKFEYLSYCLKVKEMDDEEYSYGALQEPLYRVETGNYEYRLILRCRQDARTRFARLRSDVLVKLELLDNKHVQDVVWQLQRLVAGLAEFHGETLSLLQGNSLFPIEVDLARSAFQYKSTS 376

HSP 2 Score: 237.269 bits (604), Expect = 8.634e-150
Identity = 111/146 (76.03%), Postives = 130/146 (89.04%), Query Frame = 2 
Query:   80 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            +D  DDY LEEDKMGMTVTSGSVT+ KD  NLIGISIGGGA  CPCLY+VQ+FDNTPA++DG+L SGDE+ GVN  SVK K+KVEVA++IQ+ + EVTINYNKLHADP+QGKTLDI+LKKVKHR+VENM+++TADA GLSRAILCN
Sbjct:    2 LDYEDDYFLEEDKMGMTVTSGSVTITKDNSNLIGISIGGGASLCPCLYIVQVFDNTPAARDGTLQSGDELVGVNGASVKGKTKVEVAKMIQACKEEVTINYNKLHADPQQGKTLDIVLKKVKHRLVENMSTATADALGLSRAILCN 147
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Cavefish
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:103045423])

HSP 1 Score: 340.887 bits (873), Expect = 1.048e-111
Identity = 160/230 (69.57%), Postives = 188/230 (81.74%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQ 1350
            DGLVK+L+E+ KT  L+ GL+EHSK L+RA ++LS+ +RAFG+VFS IGV+EPQ  ASEAF  F  AHRN+E+Y + +LK IKPM+ DLNT+L KAIPDTRLTI+KYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHVQDIV QL +FV  MS+Y+ +C A +KE D+FPIEVDLSR T  Y   +Q
Sbjct:  148 DGLVKKLEELEKTSELYKGLMEHSKRLLRAFFELSQTHRAFGDVFSVIGVREPQAAASEAFVKFAEAHRNIEKYGIQLLKTIKPMLHDLNTYLHKAIPDTRLTIRKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARSRFAKMRKDVLEKIELLDQKHVQDIVFQLQRFVSGMSRYYDECYAVLKEADVFPIEVDLSRTTINYSSQSQ 377

HSP 2 Score: 223.402 bits (568), Expect = 1.694e-66
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY LEEDK+G+    G+VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG+L +GDEITGVN + VK K+KVEVA++IQ+ +GE  I+YNKL ADPKQGK+LDI+LKKVKHR+VEN++S TADA GLSRAILCN
Sbjct:    7 DYELEEDKLGIPTVPGTVTLKKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTLAAGDEITGVNGKPVKGKTKVEVAKMIQAVQGEAVIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSSGTADALGLSRAILCN 147
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Cavefish
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:103045423])

HSP 1 Score: 340.887 bits (873), Expect = 1.048e-111
Identity = 160/230 (69.57%), Postives = 188/230 (81.74%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQ 1350
            DGLVK+L+E+ KT  L+ GL+EHSK L+RA ++LS+ +RAFG+VFS IGV+EPQ  ASEAF  F  AHRN+E+Y + +LK IKPM+ DLNT+L KAIPDTRLTI+KYLD KFEYLSYCLKVKEMDDEEYS  A+ EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHVQDIV QL +FV  MS+Y+ +C A +KE D+FPIEVDLSR T  Y   +Q
Sbjct:  148 DGLVKKLEELEKTSELYKGLMEHSKRLLRAFFELSQTHRAFGDVFSVIGVREPQAAASEAFVKFAEAHRNIEKYGIQLLKTIKPMLHDLNTYLHKAIPDTRLTIRKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARSRFAKMRKDVLEKIELLDQKHVQDIVFQLQRFVSGMSRYYDECYAVLKEADVFPIEVDLSRTTINYSSQSQ 377

HSP 2 Score: 223.402 bits (568), Expect = 1.694e-66
Identity = 103/141 (73.05%), Postives = 123/141 (87.23%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY LEEDK+G+    G+VTL KDAQNLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG+L +GDEITGVN + VK K+KVEVA++IQ+ +GE  I+YNKL ADPKQGK+LDI+LKKVKHR+VEN++S TADA GLSRAILCN
Sbjct:    7 DYELEEDKLGIPTVPGTVTLKKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTLAAGDEITGVNGKPVKGKTKVEVAKMIQAVQGEAVIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSSGTADALGLSRAILCN 147
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Cavefish
Match: arfip2a (arfaptin-2-like [Source:NCBI gene;Acc:103046623])

HSP 1 Score: 62.3882 bits (150), Expect = 4.256e-10
Identity = 46/196 (23.47%), Postives = 97/196 (49.49%), Query Frame = 1 
Query:  667 LVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY 1254
            L  ++D + +T   +  ++  ++ L    Y++ +  +A G+ F+ +  K P+ +  + F       R + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E+      A+ M     R+E    EY++        + ++ ++R+DV +K++ L+   V+ +  QL  F  A+S Y
Sbjct:  128 LEAQIDVLRETKLKYEHILRLAQSLTANFYNMVQTQQALGDTFADLSQKSPELQ--DEFGYNAETQRLLCKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKLYENARLEFDAYRTDLEELSTGPRDAATM----MRIEIAQQEYQI-------HKDKYERLRSDVTIKLKFLEENKVKVMHKQLLLFHNAISAY 310
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Cavefish
Match: arfip2a (arfaptin-2-like [Source:NCBI gene;Acc:103046623])

HSP 1 Score: 62.3882 bits (150), Expect = 5.812e-10
Identity = 46/196 (23.47%), Postives = 96/196 (48.98%), Query Frame = 1 
Query:  667 LVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY 1254
            L  ++D + +T   +  ++  ++ L    Y++ +  +A G+ F+ +  K P+    + F       R + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E+      A+ M     R+E    EY++        + ++ ++R+DV +K++ L+   V+ +  QL  F  A+S Y
Sbjct:  189 LEAQIDVLRETKLKYEHILRLAQSLTANFYNMVQTQQALGDTFADLSQKSPE--LQDEFGYNAETQRLLCKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKLYENARLEFDAYRTDLEELSTGPRDAATM----MRIEIAQQEYQI-------HKDKYERLRSDVTIKLKFLEENKVKVMHKQLLLFHNAISAY 371
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Cavefish
Match: arfip2a (arfaptin-2-like [Source:NCBI gene;Acc:103046623])

HSP 1 Score: 62.003 bits (149), Expect = 6.450e-10
Identity = 46/196 (23.47%), Postives = 96/196 (48.98%), Query Frame = 1 
Query:  667 LVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY 1254
            L  ++D + +T   +  ++  ++ L    Y++ +  +A G+ F+ +  K P+    + F       R + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E+      A+ M     R+E    EY++        + ++ ++R+DV +K++ L+   V+ +  QL  F  A+S Y
Sbjct:  179 LEAQIDVLRETKLKYEHILRLAQSLTANFYNMVQTQQALGDTFADLSQKSPE--LQDEFGYNAETQRLLCKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKLYENARLEFDAYRTDLEELSTGPRDAATM----MRIEIAQQEYQI-------HKDKYERLRSDVTIKLKFLEENKVKVMHKQLLLFHNAISAY 361
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Sea Lamprey
Match: pick1 (protein interacting with prkca 1 [Source:ZFIN;Acc:ZDB-GENE-061013-283])

HSP 1 Score: 215.698 bits (548), Expect = 1.500e-66
Identity = 97/141 (68.79%), Postives = 120/141 (85.11%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            D+  EEDK+G+    G V + KD QN+IGISIGGGAP CPCLYVVQ+FDN PA+KDG+L++GDEI GVN +SVK K+KVEVA++IQ+ +GEVT+ YNKL ADPK GKT+DI++KK KHR+VE+M+SSTADA GLSRAILCN
Sbjct:    6 DFDYEEDKLGIPTIPGKVVIQKDPQNMIGISIGGGAPLCPCLYVVQVFDNAPAAKDGTLMAGDEIVGVNGKSVKGKTKVEVAKMIQAVKGEVTMYYNKLQADPKLGKTMDIVMKKAKHRLVESMSSSTADALGLSRAILCN 146

HSP 2 Score: 102.834 bits (255), Expect = 4.051e-25
Identity = 48/86 (55.81%), Postives = 68/86 (79.07%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYR-AFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPM 915
            DGLVK+LDE+ +T  L+ GL+EH+K L++A ++LS+  R AFG+VFS IGV+EPQP ASEAF  F  AHR++E++ V +LK +KP+
Sbjct:  147 DGLVKKLDELERTAELYKGLMEHTKRLLKAFFELSQRVRPAFGDVFSVIGVREPQPAASEAFVKFAEAHRSIEKFGVRLLKTVKPV 232
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Sea Lamprey
Match: arfip2b (ADP-ribosylation factor interacting protein 2b [Source:ZFIN;Acc:ZDB-GENE-050417-131])

HSP 1 Score: 53.9138 bits (128), Expect = 4.919e-8
Identity = 45/189 (23.81%), Postives = 89/189 (47.09%), Query Frame = 1 
Query:  739 LVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY----HQDCLATMKETDI 1293
            L R  Y + +  R+ G+ F+ +G K P+ +  E F       R + +    +L  +   +S +NT + K + DT LTIK Y +A+ EY +Y   ++E+      ++     + +++     +          + ++ ++R DV +K++ L+   V+ +  QL  F  A+S Y     Q    T+K+ +I
Sbjct:  169 LTRHFYQV-QTQRSLGDAFADLGQKSPELQ--EEFGYNAETQRLLCKNGETLLGAVNFFVSSVNTLVNKTMEDTLLTIKMYENARVEYDAYRTDLEELSLGPRDSAV----VVKIDAAQQHF-------SAHKEKYERLRGDVAIKLKFLEENKVKVMHKQLLLFHNAISAYFAGNQQQLEQTLKQFNI 343
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Nematostella
Match: EDO38097 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDZ2])

HSP 1 Score: 301.597 bits (771), Expect = 2.368e-97
Identity = 136/220 (61.82%), Postives = 179/220 (81.36%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSR 1320
            DGLVK+LDE+ +   ++ GL++H++  + + Y LS+ ++  G++F+ IGV+E QP ASEAF IF  AHR  +R+ +D LKK+KP +SDLNT+L+KAIPDTRLTI+KY DAKFEYLSYCLKVKEMDDEEY+ +A+ E LYRVETGNY+YR++LRCRQ AR +FAK+R+DVLVK+ELLD KHVQDIV QL +FV A++ YH D    +KE ++FPIEVDL+R
Sbjct:  144 DGLVKKLDELEQNANVYKGLLDHARQYLHSFYQLSQTHKELGDIFASIGVRELQPNASEAFAIFSEAHRQFDRHGMDFLKKVKPTLSDLNTYLSKAIPDTRLTIRKYADAKFEYLSYCLKVKEMDDEEYAYAALHESLYRVETGNYDYRVVLRCRQLAREKFAKLRSDVLVKLELLDQKHVQDIVFQLQRFVSAVAGYHDDAYGILKEANVFPIEVDLTR 363

HSP 2 Score: 211.846 bits (538), Expect = 5.014e-63
Identity = 100/141 (70.92%), Postives = 119/141 (84.40%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            +Y  E D  G+    GSVTL+KD QNLIGISIGGGAP CPCLYVVQ+FDNTPASKDG+L +GDEI GVN +S++ K+KV+VAR IQ+ + EV INY KLHADPK+GKTLDI++KK+KHR+VE M+SSTADA GLSRAILCN
Sbjct:    3 EYEYEIDPRGLQGKPGSVTLIKDKQNLIGISIGGGAPLCPCLYVVQVFDNTPASKDGTLQAGDEIVGVNGKSLRGKTKVDVARAIQAVKEEVIINYVKLHADPKEGKTLDIVMKKMKHRMVEGMSSSTADALGLSRAILCN 143
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Nematostella
Match: EDO49805 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFY9])

HSP 1 Score: 48.521 bits (114), Expect = 2.804e-6
Identity = 45/204 (22.06%), Postives = 92/204 (45.10%), Query Frame = 1 
Query:  649 DTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEP--LYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY 1254
            DT L+  +  L E      L   ++  S+ L+     + +  ++ G+ FS +G+K P+ +  + F       +++ +    +L  +    ++L T + K + D+  T+K Y  A+ EY +Y        D E + +  + P    R E    ++       +  + +F ++RND+ +K+  LD   V+ +  QL  F  A++ Y
Sbjct:   33 DTELEAQILLLRETQAKYAL---MLRQSRQLMLQFQAMLQTQKSLGDTFSDLGMKSPELQ--DEFNYNAETQKSLIKNGEVLLGALSFFNTNLETLVHKTMEDSVTTVKAYESARIEYDAY------RSDLEAAQAGPRTPATAARAEETREQF-------EKQKAKFDRLRNDLTIKLRFLDENKVKVMHKQLLLFNNAITAY 218
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Medaka
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:101171334])

HSP 1 Score: 337.806 bits (865), Expect = 2.539e-110
Identity = 158/229 (69.00%), Postives = 188/229 (82.10%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSN 1347
            DGLVKRL+E+ KT  L+ GL+EH+K L+RA ++LS+ +RAFG+VFS IGV+EPQ  ASEAF  F +AHRN+E+Y + +LK IKPM+ DLNT+L KAIPDT+LTI+KYLD KFEYLSYCLKVKEMDDEEYS+ AM EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHVQDIV QL +FV  MS Y+ +C A ++E D+FPIEVDLSR T  Y   +
Sbjct:  161 DGLVKRLEELEKTAELYKGLMEHTKRLLRAFFELSQTHRAFGDVFSVIGVREPQAAASEAFVKFADAHRNIEKYGIQLLKTIKPMLHDLNTYLHKAIPDTKLTIRKYLDVKFEYLSYCLKVKEMDDEEYSSIAMGEPLYRVSTGNYEYRLILRCRQEARARFAKMRKDVLEKIELLDQKHVQDIVFQLQRFVSGMSHYYDECYAVLREADVFPIEVDLSRTTINYSSQS 389

HSP 2 Score: 218.394 bits (555), Expect = 1.481e-64
Identity = 101/141 (71.63%), Postives = 122/141 (86.52%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY LEEDK+G+    G+VTL KDA NLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG+L +GDEITGVN + VK K+KVEVA++IQ+ +GE  I+YNKL ADPKQGK+LDI+LKKVKHR+VEN+++ TADA GLSRAILCN
Sbjct:   20 DYDLEEDKLGIPTVPGTVTLKKDANNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTLAAGDEITGVNGKPVKGKTKVEVAKMIQAVQGESVIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSAGTADALGLSRAILCN 160
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Medaka
Match: pick1 (protein interacting with PRKCA 1 [Source:NCBI gene;Acc:101171334])

HSP 1 Score: 337.806 bits (865), Expect = 2.539e-110
Identity = 158/229 (69.00%), Postives = 188/229 (82.10%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSN 1347
            DGLVKRL+E+ KT  L+ GL+EH+K L+RA ++LS+ +RAFG+VFS IGV+EPQ  ASEAF  F +AHRN+E+Y + +LK IKPM+ DLNT+L KAIPDT+LTI+KYLD KFEYLSYCLKVKEMDDEEYS+ AM EPLYRV TGNYEYRLILRCRQ+AR RFAKMR DVL KIELLD KHVQDIV QL +FV  MS Y+ +C A ++E D+FPIEVDLSR T  Y   +
Sbjct:  161 DGLVKRLEELEKTAELYKGLMEHTKRLLRAFFELSQTHRAFGDVFSVIGVREPQAAASEAFVKFADAHRNIEKYGIQLLKTIKPMLHDLNTYLHKAIPDTKLTIRKYLDVKFEYLSYCLKVKEMDDEEYSSIAMGEPLYRVSTGNYEYRLILRCRQEARARFAKMRKDVLEKIELLDQKHVQDIVFQLQRFVSGMSHYYDECYAVLREADVFPIEVDLSRTTINYSSQS 389

HSP 2 Score: 218.394 bits (555), Expect = 1.481e-64
Identity = 101/141 (71.63%), Postives = 122/141 (86.52%), Query Frame = 2 
Query:   95 DYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            DY LEEDK+G+    G+VTL KDA NLIGISIGGGA YCPCLY+VQ+FDNTPA+ DG+L +GDEITGVN + VK K+KVEVA++IQ+ +GE  I+YNKL ADPKQGK+LDI+LKKVKHR+VEN+++ TADA GLSRAILCN
Sbjct:   20 DYDLEEDKLGIPTVPGTVTLKKDANNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTLAAGDEITGVNGKPVKGKTKVEVAKMIQAVQGESVIHYNKLQADPKQGKSLDIVLKKVKHRLVENLSAGTADALGLSRAILCN 160
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Medaka
Match: arfip1 (ADP ribosylation factor interacting protein 1 [Source:NCBI gene;Acc:101159971])

HSP 1 Score: 60.4622 bits (145), Expect = 2.964e-9
Identity = 44/190 (23.16%), Postives = 93/190 (48.95%), Query Frame = 1 
Query:  718 LVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKET 1287
            +++ ++ L   +  + +  R  G+ F+ + +K P+    E F    +  + + +    +L  +   IS +NT + K I DT + IK+Y  A+ EY +Y   ++E++     A+ M     ++E    ++++        R ++ +MR DV VK++ L+   V+ +  QL  F  A++ Y+      ++ET
Sbjct:  270 VIKLTQTLANQLSQIIQTQRQLGDAFADLSLKSPE--LHEEFGYNADTQKLLSKNGETLLGAMNFFISSVNTLVDKTIEDTMINIKQYETARVEYDAYRTDLEELNLGPRDANTMP----KIEQSQQQFQI-------HREKYERMRADVSVKLKFLEENKVKVLHNQLILFHNAIAAYYAGNQQQLEET 446
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Medaka
Match: arfip1 (ADP ribosylation factor interacting protein 1 [Source:NCBI gene;Acc:101159971])

HSP 1 Score: 60.4622 bits (145), Expect = 2.964e-9
Identity = 44/190 (23.16%), Postives = 93/190 (48.95%), Query Frame = 1 
Query:  718 LVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKET 1287
            +++ ++ L   +  + +  R  G+ F+ + +K P+    E F    +  + + +    +L  +   IS +NT + K I DT + IK+Y  A+ EY +Y   ++E++     A+ M     ++E    ++++        R ++ +MR DV VK++ L+   V+ +  QL  F  A++ Y+      ++ET
Sbjct:  270 VIKLTQTLANQLSQIIQTQRQLGDAFADLSLKSPE--LHEEFGYNADTQKLLSKNGETLLGAMNFFISSVNTLVDKTIEDTMINIKQYETARVEYDAYRTDLEELNLGPRDANTMP----KIEQSQQQFQI-------HREKYERMRADVSVKLKFLEENKVKVLHNQLILFHNAIAAYYAGNQQQLEET 446
[Close]
BLAST of PRKCA-binding protein vs. Ensembl Medaka
Match: arfip2a (arfaptin-2 [Source:NCBI gene;Acc:101175336])

HSP 1 Score: 58.5362 bits (140), Expect = 6.412e-9
Identity = 41/172 (23.84%), Postives = 85/172 (49.42%), Query Frame = 1 
Query:  739 LVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKY 1254
            L+     + +  +A G+ F+ +  K P+ +  + F       + + +    +L  I   +S +NT + K + DT +TIK Y +A+ E+ +Y   ++E+      A++M     R+E   ++Y++        R ++ ++R+DV +K++ L+   V+ +  QL  F  A S Y
Sbjct:  148 LISHFQSMVETQQALGDTFTDLSQKSPELQ--DEFGYNAETQKLLCKNGESLLGAINFFVSSVNTLVNKTMEDTLITIKHYENARLEFDAYRSDLEELSMGPRDAASM----VRIEMAQHDYQI-------HRDKYERLRSDVTIKLKFLEENKVKVMHKQLLLFHNATSAY 306
[Close]
BLAST of PRKCA-binding protein vs. Planmine SMEST
Match: SMESG000005998.1 (SMESG000005998.1)

HSP 1 Score: 631.328 bits (1627), Expect = 0.000e+0
Identity = 323/324 (99.69%), Postives = 323/324 (99.69%), Query Frame = 1 
Query:  598 VACLRLYKFWDLFLLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFXXXXXXXXXXXXXXXXXNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI 1569
            V CLRLYKFWDLFLLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFDDVDDEDDEILDTTECDNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI
Sbjct:  191 VTCLRLYKFWDLFLLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFDDVDDEDDEILDTTECDNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI 514

HSP 2 Score: 320.472 bits (820), Expect = 0.000e+0
Identity = 163/199 (81.91%), Postives = 165/199 (82.91%), Query Frame = 2 
Query:   80 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDII------------------------------LKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKNSLCIRNREF 586
            MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDII                              LKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN  C+R  +F
Sbjct:    1 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRLVERLESSTADALGLSRAILVNVLKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKNVTCLRLYKF 199
[Close]
BLAST of PRKCA-binding protein vs. Planmine SMEST
Match: SMESG000005998.1 (SMESG000005998.1)

HSP 1 Score: 587.415 bits (1513), Expect = 0.000e+0
Identity = 302/309 (97.73%), Postives = 307/309 (99.35%), Query Frame = 1 
Query:  643 INDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFXXXXXXXXXXXXXXXXXNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI 1569
            ++  +++GLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFDDVDDEDDEILDTTECDNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI
Sbjct:  186 MSSKNVNGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFDDVDDEDDEILDTTECDNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI 494

HSP 2 Score: 314.694 bits (805), Expect = 0.000e+0
Identity = 160/190 (84.21%), Postives = 160/190 (84.21%), Query Frame = 2 
Query:   80 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDII------------------------------LKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN 559
            MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDII                              LKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN
Sbjct:    1 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRLVERLESSTADALGLSRAILVNVLKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKN 190
[Close]
BLAST of PRKCA-binding protein vs. Planmine SMEST
Match: SMESG000005998.1 (SMESG000005998.1)

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1 
Query:  661 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFXXXXXXXXXXXXXXXXXNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI 1569
            DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFDDVDDEDDEILDTTECDNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI
Sbjct:  147 DGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSNQFDDVDDEDDEILDTTECDNKPNKNDTVKNVVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLI 449

HSP 2 Score: 293.123 bits (749), Expect = 7.951e-93
Identity = 150/191 (78.53%), Postives = 160/191 (83.77%), Query Frame = 2 
Query:   80 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSMSSKNSL---CIRNREFLL--IL*LACAYINFGICF 637
            MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHR+VE + SSTADA GLSRAIL N   ++    M+    L    + + +FL+  I  L+  Y  FG  F
Sbjct:    1 MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRLVERLESSTADALGLSRAILVNDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVF 191
[Close]
BLAST of PRKCA-binding protein vs. Planmine SMEST
Match: SMESG000013976.1 (SMESG000013976.1)

HSP 1 Score: 243.047 bits (619), Expect = 8.460e-108
Identity = 124/237 (52.32%), Postives = 161/237 (67.93%), Query Frame = 1 
Query:  637 LLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYDKSN 1347
            +L+ND+    L+KRL+ +N+T  LF  L            + + +++   EVF  IGVKEPQ  ASEAF IF    R M++Y+     ++  ++ DL  FL K IPDT+LTIKKYL+AKFEYLSYCLKVKEMDDEE    A+ E LYR+ETGNYEYRL+LRCRQ++R +F+KMRNDVL K+ELLDNKHVQDIV  L KF+  + +Y+ D  +  K   IFPIE+D+S  T   D+ N
Sbjct:  162 ILVNDS----LMKRLELLNQTSHLFEDLSNFISKFNEESKESAIIHKKMAEVFENIGVKEPQANASEAFLIFAKGQRCMQKYSDFTYAEVNKIVDDLKIFLKKVIPDTKLTIKKYLEAKFEYLSYCLKVKEMDDEESCYVALHEALYRIETGNYEYRLLLRCRQNSRKKFSKMRNDVLEKMELLDNKHVQDIVKNLGKFMDILKQYYNDNYSAWKNAQIFPIEIDISSLTHINDEFN 394

HSP 2 Score: 168.318 bits (425), Expect = 8.460e-108
Identity = 89/145 (61.38%), Postives = 114/145 (78.62%), Query Frame = 2 
Query:   83 DLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCN 517
            D+ + +P +EDK GM VTSGSV L KD  +LIGISIGGG PYCPC+Y+VQIFDNT A+KDG+L +GDE+T ++++SVK  S+ EVA+ IQ    +VTI YNKLH D  QGK++++I K+ KHR+VEN+ SSTAD  GLSRAIL N
Sbjct:   22 DIMNSFP-QEDKCGMRVTSGSVVLNKDKHSLIGISIGGGIPYCPCIYIVQIFDNTVAAKDGTLEAGDEVTAIDKKSVKGVSRGEVAKWIQKAENKVTIYYNKLHLDKNQGKSVEMIFKRFKHRLVENIDSSTADFLGLSRAILVN 165
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BLAST of PRKCA-binding protein vs. Planmine SMEST
Match: SMESG000050775.1 (SMESG000050775.1)

HSP 1 Score: 229.95 bits (585), Expect = 6.413e-69
Identity = 110/234 (47.01%), Postives = 163/234 (69.66%), Query Frame = 1 
Query:  637 LLINDTHLDGLVKRLDEMNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASEAFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLDAKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDARIRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKETDIFPIEVDLSRHTFTYD 1338
            +L+NDT    +VK+   + +T  ++  LV++    + A  ++  V++ FG++  +IG++E Q  A+EAF  FG  H+ ME+  ++ LKK+K +I +L  F++ AIPD  LTIKKYLD KFEYL+YCLK+KEMDDEEY   +M E LYR+ETGNYEYR  LRCRQ+AR RFA+ R +VL+K++LLDNK+VQDI   L   V+ +S++H      +K   +FPIE+D++  TF+++
Sbjct:  139 ILVNDT----MVKKFSNLKETANVYKNLVKYLSNFLHANLEMGLVHKVFGDLNIEIGIRELQVSANEAFCKFGKIHQTMEKNMINSLKKLKQVILNLQQFVSIAIPDIYLTIKKYLDVKFEYLAYCLKIKEMDDEEYGFYSMHESLYRLETGNYEYRFNLRCRQEARRRFAQERTNVLIKLDLLDNKYVQDITTNLQTLVQCLSEFHSANFHLLKNIRLFPIEIDIAHTTFSHE 368

HSP 2 Score: 164.851 bits (416), Expect = 1.019e-44
Identity = 81/142 (57.04%), Postives = 104/142 (73.24%), Query Frame = 2 
Query:  122 GMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVVQIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTINYNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSIENCSSM 547
            GM +TSGSVTL K   NLIGISIGGGAP CP LYVVQ+FDN+ ++KDG+L +GDE+  VN  SVK+ ++ E A+LIQ  +  VTINYNKLH D   GK+ DI LKK+KH+ V+ + SS AD  G+SRAIL N   ++  S++
Sbjct:   11 GMRITSGSVTLEKKRNNLIGISIGGGAPLCPALYVVQVFDNSSSAKDGTLEAGDELVSVNGISVKNYTRTETAKLIQESKDSVTINYNKLHVDCIHGKSCDINLKKIKHKFVDKINSSVADLIGMSRAILVNDTMVKKFSNL 152
The following BLAST results are available for this feature:
BLAST of PRKCA-binding protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PICK11.252e-10767.56protein interacting with PRKCA 1 [Source:HGNC Symb... [more]
PICK11.252e-10767.56protein interacting with PRKCA 1 [Source:HGNC Symb... [more]
PICK15.343e-6973.05protein interacting with PRKCA 1 [Source:HGNC Symb... [more]
PICK14.757e-6171.32protein interacting with PRKCA 1 [Source:HGNC Symb... [more]
PICK11.230e-4970.27protein interacting with PRKCA 1 [Source:HGNC Symb... [more]
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BLAST of PRKCA-binding protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Y57G11C.221.521e-6352.04pep chromosome:WBcel235:IV:14837112:14853662:1 gen... [more]
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BLAST of PRKCA-binding protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PICK12.041e-8354.78gene:FBgn0032447 transcript:FBtr0340542[more]
PICK12.041e-8354.78gene:FBgn0032447 transcript:FBtr0340544[more]
PICK12.041e-8354.78gene:FBgn0032447 transcript:FBtr0080396[more]
PICK13.619e-8354.78gene:FBgn0032447 transcript:FBtr0080395[more]
PICK12.120e-8254.78gene:FBgn0032447 transcript:FBtr0340543[more]
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BLAST of PRKCA-binding protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pick14.195e-10969.33protein interacting with prkca 1 [Source:ZFIN;Acc:... [more]
fhdc39.635e-1024.48FH2 domain containing 3 [Source:ZFIN;Acc:ZDB-GENE-... [more]
arfip2b1.302e-924.48ADP-ribosylation factor interacting protein 2b [So... [more]
arfip2b1.302e-924.48ADP-ribosylation factor interacting protein 2b [So... [more]
arfip2b1.302e-924.48ADP-ribosylation factor interacting protein 2b [So... [more]
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BLAST of PRKCA-binding protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pick12.088e-11067.53protein interacting with PRKCA 1 [Source:NCBI gene... [more]
pick12.342e-11067.53protein interacting with PRKCA 1 [Source:NCBI gene... [more]
pick14.053e-11067.53protein interacting with PRKCA 1 [Source:NCBI gene... [more]
pick14.475e-7251.52protein interacting with PRKCA 1 [Source:NCBI gene... [more]
ARFIP21.973e-1025.15ADP ribosylation factor interacting protein 2 [Sou... [more]
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BLAST of PRKCA-binding protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pick11.279e-10868.00protein interacting with C kinase 1 [Source:MGI Sy... [more]
Pick11.279e-10868.00protein interacting with C kinase 1 [Source:MGI Sy... [more]
Pick11.279e-10868.00protein interacting with C kinase 1 [Source:MGI Sy... [more]
Gm494864.885e-8570.56predicted gene, 49486 [Source:MGI Symbol;Acc:MGI:6... [more]
Arfip22.738e-924.02ADP-ribosylation factor interacting protein 2 [Sou... [more]
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BLAST of PRKCA-binding protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9EP80|PICK1_RAT7.140e-10868.00PRKCA-binding protein OS=Rattus norvegicus OX=1011... [more]
sp|Q2T9M1|PICK1_BOVIN2.539e-10766.38PRKCA-binding protein OS=Bos taurus OX=9913 GN=PIC... [more]
sp|Q5REH1|PICK1_PONAB5.575e-10767.56PRKCA-binding protein OS=Pongo abelii OX=9601 GN=P... [more]
sp|Q9NRD5|PICK1_HUMAN6.011e-10767.56PRKCA-binding protein OS=Homo sapiens OX=9606 GN=P... [more]
sp|Q4R7Q5|PICK1_MACFA2.651e-10667.11PRKCA-binding protein OS=Macaca fascicularis OX=95... [more]
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BLAST of PRKCA-binding protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U6JA561.880e-15757.37PRKCA-binding protein OS=Echinococcus granulosus O... [more]
A0A158R7N26.444e-15768.24Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
R7VGQ01.409e-15668.16Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A0X3P4P25.074e-15667.38PRKCA-binding protein OS=Schistocephalus solidus O... [more]
A0A2J7Q1Z08.634e-15062.03PRKCA-binding protein OS=Cryptotermes secundus OX=... [more]
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BLAST of PRKCA-binding protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pick11.048e-11169.57protein interacting with PRKCA 1 [Source:NCBI gene... [more]
pick11.048e-11169.57protein interacting with PRKCA 1 [Source:NCBI gene... [more]
arfip2a4.256e-1023.47arfaptin-2-like [Source:NCBI gene;Acc:103046623][more]
arfip2a5.812e-1023.47arfaptin-2-like [Source:NCBI gene;Acc:103046623][more]
arfip2a6.450e-1023.47arfaptin-2-like [Source:NCBI gene;Acc:103046623][more]
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BLAST of PRKCA-binding protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pick11.500e-6668.79protein interacting with prkca 1 [Source:ZFIN;Acc:... [more]
arfip2b4.919e-823.81ADP-ribosylation factor interacting protein 2b [So... [more]
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BLAST of PRKCA-binding protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PRKCA-binding protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO380972.368e-9761.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO498052.804e-622.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of PRKCA-binding protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pick12.539e-11069.00protein interacting with PRKCA 1 [Source:NCBI gene... [more]
pick12.539e-11069.00protein interacting with PRKCA 1 [Source:NCBI gene... [more]
arfip12.964e-923.16ADP ribosylation factor interacting protein 1 [Sou... [more]
arfip12.964e-923.16ADP ribosylation factor interacting protein 1 [Sou... [more]
arfip2a6.412e-923.84arfaptin-2 [Source:NCBI gene;Acc:101175336][more]
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BLAST of PRKCA-binding protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005998.10.000e+099.69SMESG000005998.1[more]
SMESG000005998.10.000e+097.73SMESG000005998.1[more]
SMESG000005998.17.951e-93100.00SMESG000005998.1[more]
SMESG000013976.18.460e-10852.32SMESG000013976.1[more]
SMESG000050775.16.413e-6947.01SMESG000050775.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021178 ID=SMED30021178|Name=PRKCA-binding protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1795bp
TTATTTTTAAAAAATTGAAAAGGATTTTCAATTAACCTTTTAATTTGATT
AATTATAAATTTTATCGTTTTAACAACAAATGGATCTCTACGATGATTAT
CCACTAGAAGAGGATAAAATGGGAATGACAGTAACCAGTGGATCAGTTAC
TTTGTTGAAAGATGCTCAGAATTTAATTGGTATAAGCATTGGTGGTGGTG
CACCTTATTGTCCATGTTTATATGTGGTGCAAATATTTGACAACACACCT
GCATCTAAAGACGGAAGCTTGTTATCTGGCGATGAAATTACGGGGGTTAA
TCAGCAGTCTGTGAAGAGTAAAAGTAAAGTCGAAGTCGCCAGATTGATTC
AGAGTTACAGAGGGGAAGTTACAATTAACTACAATAAATTACACGCTGAT
CCCAAGCAAGGAAAGACATTAGATATCATTTTGAAGAAAGTCAAACATAG
AGTTGTGGAGAACATGACCTCTAGTACTGCTGATGCCTTTGGTCTAAGTA
GAGCAATTCTGTGCAATGTGTTGTCAATCGAAAACTGTAGTAGCATGTCG
TCAAAAAATTCCCTTTGTATAAGAAATCGTGAATTTTTGCTTATTTTGTA
GCTTGCTTGCGCTTATATAAATTTTGGGATTTGTTTTTATTAATTAATGA
CACACATCTAGATGGGCTTGTAAAAAGATTGGATGAAATGAATAAAACCG
GTGGACTGTTTTTTGGATTAGTAGAGCACTCGAAATTTTTAGTGAGAGCA
ATTTATGATCTTTCCAAAGTTTATAGAGCTTTTGGAGAAGTTTTTTCACA
GATCGGAGTGAAAGAACCTCAACCACGAGCTAGTGAGGCATTTACAATTT
TCGGAAATGCTCATAGAAACATGGAGCGTTATGCCGTAGATATGCTGAAA
AAAATAAAACCAATGATATCAGATTTGAATACTTTCTTGACTAAGGCTAT
TCCAGATACTAGATTAACAATAAAAAAATATCTTGATGCAAAATTTGAAT
ATCTGTCTTATTGCTTGAAAGTGAAAGAAATGGATGATGAGGAATACAGT
GCTTCCGCAATGCAGGAGCCTTTATATAGGGTTGAAACAGGGAATTATGA
ATACAGACTAATTTTGCGATGTCGCCAAGATGCTCGAATCCGATTTGCTA
AAATGCGAAATGATGTTTTGGTTAAAATCGAGCTGTTGGATAATAAACAC
GTTCAGGATATTGTTTGTCAACTTGATAAATTTGTGAAAGCTATGAGCAA
ATATCATCAAGATTGCTTAGCGACCATGAAAGAAACCGACATTTTCCCAA
TAGAGGTCGACCTGTCCAGGCATACATTCACTTATGATAAATCCAATCAG
TTTGATGATGTGGACGATGAAGATGATGAAATCCTCGATACGACTGAATG
TGATAATAAACCAAATAAAAACGACACCGTTAAGAATGTCGTTGAGAAAG
ATTCGGATTTACTGAAAGATTTCGGTGATTCTTTCGCAGATTTGAATTTT
AGTTCCAATGAAAATCAAAATCCCAATACCAAACAAGGCGAAACTGTGGA
TCTTTTGGGCGATTTAATTTCTACAAATTGATGATGTAATACTAGTACAA
ATATGTATTTTTTATTTGTTGTGCATTTTTACTAGAGTAACATAATTGTT
GGATTTGGTGATTTGGTTATCTCTCTATTATACATCATATATTGTTGATT
TTGTTCATTTGTCGTTGCGAATCATTAATGTTTTTCTCCAGTTTGTTGTT
CAACGGTTAGTACGTAGTTAGGGGCTTCCTGTCATATAACACACA
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protein sequence of SMED30021178-orf-1

>SMED30021178-orf-1 ID=SMED30021178-orf-1|Name=SMED30021178-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=298bp
MNKTGGLFFGLVEHSKFLVRAIYDLSKVYRAFGEVFSQIGVKEPQPRASE
AFTIFGNAHRNMERYAVDMLKKIKPMISDLNTFLTKAIPDTRLTIKKYLD
AKFEYLSYCLKVKEMDDEEYSASAMQEPLYRVETGNYEYRLILRCRQDAR
IRFAKMRNDVLVKIELLDNKHVQDIVCQLDKFVKAMSKYHQDCLATMKET
DIFPIEVDLSRHTFTYDKSNQFDDVDDEDDEILDTTECDNKPNKNDTVKN
VVEKDSDLLKDFGDSFADLNFSSNENQNPNTKQGETVDLLGDLISTN*
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protein sequence of SMED30021178-orf-2

>SMED30021178-orf-2 ID=SMED30021178-orf-2|Name=SMED30021178-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=174bp
MDLYDDYPLEEDKMGMTVTSGSVTLLKDAQNLIGISIGGGAPYCPCLYVV
QIFDNTPASKDGSLLSGDEITGVNQQSVKSKSKVEVARLIQSYRGEVTIN
YNKLHADPKQGKTLDIILKKVKHRVVENMTSSTADAFGLSRAILCNVLSI
ENCSSMSSKNSLCIRNREFLLIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000099neuron
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR030798Arfaptin_fam
IPR037959PICK1_BAR
IPR001478PDZ
IPR010504AH_dom
IPR027267AH/BAR_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0019904protein domain specific binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010504Arfaptin homology (AH) domainSMARTSM01015Arfaptin_2coord: 1..197
e-value: 5.1E-76
score: 268.5
IPR010504Arfaptin homology (AH) domainPFAMPF06456Arfaptincoord: 7..197
e-value: 4.2E-62
score: 209.7
IPR010504Arfaptin homology (AH) domainPROSITEPS50870AHcoord: 1..202
score: 36.428
IPR027267AH/BAR domain superfamilyGENE3DG3DSA:1.20.1270.60coord: 1..198
e-value: 1.7E-58
score: 199.6
IPR027267AH/BAR domain superfamilySUPERFAMILYSSF103657BAR/IMD domain-likecoord: 4..198
NoneNo IPR availablePANTHERPTHR12141:SF1PRKCA-BINDING PROTEINcoord: 2..235
IPR030798Arfaptin familyPANTHERPTHR12141ARFAPTIN-RELATEDADP-RIBOSYLATION FACTOR INTERACTING PROTEIN/PROTEIN KINASE C ALPHA BINDING PROTEINcoord: 2..235
IPR037959PICK1, BAR domainCDDcd07659BAR_PICK1coord: 1..205
e-value: 2.20748E-129
score: 364.724