Calmodulin, putative

Overview
NameCalmodulin, putative
Smed IDSMED30021154
Length (bp)754
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calmodulin, putative (SMED30021154) t-SNE clustered cells

Violin plots show distribution of expression levels for Calmodulin, putative (SMED30021154) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calmodulin, putative (SMED30021154) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calmodulin, putative (SMED30021154) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021154

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021154h1SMcG0011410 Contig52168uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021154h1SMcG0011410 Contig52168newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
goblet cellSMED30021154h1SMcG0011410 dd_Smed_v6_603_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30021154h1SMcG0011410 dd_Smed_v4_603_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calmodulin, putative vs. Ensembl Human
Match: CALM2 (calmodulin 2 [Source:HGNC Symbol;Acc:HGNC:1445])

HSP 1 Score: 124.02 bits (310), Expect = 5.067e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Human
Match: CALM2 (calmodulin 2 [Source:HGNC Symbol;Acc:HGNC:1445])

HSP 1 Score: 124.02 bits (310), Expect = 5.067e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Human
Match: CALM1 (calmodulin 1 [Source:HGNC Symbol;Acc:HGNC:1442])

HSP 1 Score: 124.02 bits (310), Expect = 5.067e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Human
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 124.02 bits (310), Expect = 5.067e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Human
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 124.02 bits (310), Expect = 5.067e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 121.324 bits (303), Expect = 1.831e-34
Identity = 65/143 (45.45%), Postives = 94/143 (65.73%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTM 145          

HSP 2 Score: 53.9138 bits (128), Expect = 6.246e-9
Identity = 26/61 (42.62%), Postives = 41/61 (67.21%), Query Frame = 1
Query:  205 KEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFM 387
            +EAF +FD D NG+I   EL  +  ++GE  +D ++D MI +AD ++ DGQ+ ++EFV  M
Sbjct:   87 REAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREAD-IDGDGQVNYEEFVTMM 146          
BLAST of Calmodulin, putative vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 109.383 bits (272), Expect = 9.865e-30
Identity = 60/144 (41.67%), Postives = 90/144 (62.50%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
             LT +E   ++EAF +FD D NG I  +EL     S+G++P++ ++  MI + D ++ +GQIEF EF   M+   +   + +E ++ AFRVFD+DGNG I  +E R  M ++G   +E E+D MI E D DGDG I ++EF KM
Sbjct:   17 QLTPEEIDEFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVD-IDGNGQIEFPEFCVMMKR--MMKETDSEMIREAFRVFDKDGNGVITAQEFRYFMVHMGMQFSEEEVDEMIKEVDVDGDGEIDYEEFVKM 157          

HSP 2 Score: 46.595 bits (109), Expect = 2.908e-6
Identity = 26/73 (35.62%), Postives = 41/73 (56.16%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNN 396
            + E +    +EAF +FD D NG I  QE       +G   S+ ++D MI + D ++ DG+I+++EFV  M N 
Sbjct:   90 MKETDSEMIREAFRVFDKDGNGVITAQEFRYFMVHMGMQFSEEEVDEMIKEVD-VDGDGEIDYEEFVKMMSNQ 161          
BLAST of Calmodulin, putative vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 109.383 bits (272), Expect = 1.812e-29
Identity = 60/144 (41.67%), Postives = 90/144 (62.50%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
             LT +E   ++EAF +FD D NG I  +EL     S+G++P++ ++  MI + D ++ +GQIEF EF   M+   +   + +E ++ AFRVFD+DGNG I  +E R  M ++G   +E E+D MI E D DGDG I ++EF KM
Sbjct:   36 QLTPEEIDEFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVD-IDGNGQIEFPEFCVMMKR--MMKETDSEMIREAFRVFDKDGNGVITAQEFRYFMVHMGMQFSEEEVDEMIKEVDVDGDGEIDYEEFVKM 176          

HSP 2 Score: 46.595 bits (109), Expect = 2.960e-6
Identity = 26/73 (35.62%), Postives = 41/73 (56.16%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNN 396
            + E +    +EAF +FD D NG I  QE       +G   S+ ++D MI + D ++ DG+I+++EFV  M N 
Sbjct:  109 MKETDSEMIREAFRVFDKDGNGVITAQEFRYFMVHMGMQFSEEEVDEMIKEVD-VDGDGEIDYEEFVKMMSNQ 180          
BLAST of Calmodulin, putative vs. Ensembl Celegans
Match: cal-2 (CALmodulin related genes; Calmodulin-like protein [Source:UniProtKB/TrEMBL;Acc:G5EEG0])

HSP 1 Score: 105.916 bits (263), Expect = 3.373e-28
Identity = 59/146 (40.41%), Postives = 89/146 (60.96%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            L E+E   YK AF LFD D NG I  +EL     S+G++P++ +L  M+ + D ++  G I+F EF   M+   +   + +E ++ AFRVFDRDGNG I  +E R  M ++G+  ++ E+D +I E D DGDG I ++EF+  F A
Sbjct:   29 LNEEEIMEYKAAFRLFDKDGNGSISSKELGVAMRSLGQNPTEQELLDMVNEVD-IDGSGTIDFGEFCQMMKR--MNKENDSEMIREAFRVFDRDGNGFITADEFRYFMTHMGDQFSDQEVDEIIAEIDIDGDGQIDYEEFASTFSA 171          
BLAST of Calmodulin, putative vs. Ensembl Celegans
Match: cal-3 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:U4PQW9])

HSP 1 Score: 98.5969 bits (244), Expect = 1.543e-25
Identity = 59/145 (40.69%), Postives = 92/145 (63.45%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMF 609
             LTE+E   +KEAF LFD D NG I  +EL     ++G++P++ Q+  +I   D L+ +GQ+EF EF   M+   +   + +E ++ AF++FDRDGNG I   E ++ M N+G    E E++ M++E DCDG+G I ++EF K+F
Sbjct:   22 QLTEEEIHEFKEAFLLFDKDGNGTISIKELGVAMRALGQNPTEQQMMEIIHDVD-LDGNGQVEFPEFCVMMKR--IMKETDSEMIREAFKIFDRDGNGVITANEFKLFMINMGMCFDEVEVEEMMNEVDCDGNGEIDYEEFVKIF 163          
BLAST of Calmodulin, putative vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304964)

HSP 1 Score: 121.709 bits (304), Expect = 1.956e-34
Identity = 65/146 (44.52%), Postives = 95/146 (65.07%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M  +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTS 148          
BLAST of Calmodulin, putative vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0088001)

HSP 1 Score: 121.709 bits (304), Expect = 1.956e-34
Identity = 65/146 (44.52%), Postives = 95/146 (65.07%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M  +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTS 148          
BLAST of Calmodulin, putative vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304963)

HSP 1 Score: 121.709 bits (304), Expect = 1.956e-34
Identity = 65/146 (44.52%), Postives = 95/146 (65.07%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M  +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTS 148          
BLAST of Calmodulin, putative vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0088002)

HSP 1 Score: 121.709 bits (304), Expect = 1.956e-34
Identity = 65/146 (44.52%), Postives = 95/146 (65.07%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M  +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTS 148          
BLAST of Calmodulin, putative vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0345018)

HSP 1 Score: 121.709 bits (304), Expect = 1.956e-34
Identity = 65/146 (44.52%), Postives = 95/146 (65.07%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M  +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTS 148          
BLAST of Calmodulin, putative vs. Ensembl Zebrafish
Match: calm2a (calmodulin 2a (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030804-3])

HSP 1 Score: 124.02 bits (310), Expect = 2.958e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Zebrafish
Match: calm3a (calmodulin 3a (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030131-5590])

HSP 1 Score: 124.02 bits (310), Expect = 3.224e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Zebrafish
Match: calm3b (calmodulin 3b (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030131-527])

HSP 1 Score: 124.02 bits (310), Expect = 3.224e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Zebrafish
Match: calm1b (calmodulin 1b [Source:ZFIN;Acc:ZDB-GENE-030804-2])

HSP 1 Score: 124.02 bits (310), Expect = 3.224e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Zebrafish
Match: calm1b (calmodulin 1b [Source:ZFIN;Acc:ZDB-GENE-030804-2])

HSP 1 Score: 124.02 bits (310), Expect = 3.224e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Xenopus
Match: CALM2 (calmodulin 1 (phosphorylase kinase, delta) [Source:NCBI gene;Acc:100496298])

HSP 1 Score: 124.02 bits (310), Expect = 4.594e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Xenopus
Match: CALM2 (calmodulin 1 (phosphorylase kinase, delta) [Source:NCBI gene;Acc:100496298])

HSP 1 Score: 124.02 bits (310), Expect = 4.594e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Xenopus
Match: calm2 (calmodulin 2 (phosphorylase kinase, delta) [Source:NCBI gene;Acc:493522])

HSP 1 Score: 124.02 bits (310), Expect = 4.594e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Xenopus
Match: calm2 (calmodulin 2 (phosphorylase kinase, delta) [Source:NCBI gene;Acc:493522])

HSP 1 Score: 124.02 bits (310), Expect = 4.594e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Xenopus
Match: CALM2 (calmodulin 1 (phosphorylase kinase, delta) [Source:NCBI gene;Acc:100496298])

HSP 1 Score: 119.013 bits (297), Expect = 3.215e-33
Identity = 63/140 (45.00%), Postives = 92/140 (65.71%), Query Frame = 1
Query:  199 AYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAE 618
             +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A+
Sbjct:    5 TFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 142          
BLAST of Calmodulin, putative vs. Ensembl Mouse
Match: Calm3 (calmodulin 3 [Source:MGI Symbol;Acc:MGI:103249])

HSP 1 Score: 124.02 bits (310), Expect = 3.479e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Mouse
Match: Calm1 (calmodulin 1 [Source:MGI Symbol;Acc:MGI:88251])

HSP 1 Score: 124.02 bits (310), Expect = 3.479e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Mouse
Match: Calm2 (calmodulin 2 [Source:MGI Symbol;Acc:MGI:103250])

HSP 1 Score: 124.02 bits (310), Expect = 3.479e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Mouse
Match: Calm1 (calmodulin 1 [Source:MGI Symbol;Acc:MGI:88251])

HSP 1 Score: 124.02 bits (310), Expect = 1.186e-34
Identity = 66/148 (44.59%), Postives = 97/148 (65.54%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAE 618
             LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A+
Sbjct:   52 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 197          
BLAST of Calmodulin, putative vs. Ensembl Mouse
Match: Calml3 (calmodulin-like 3 [Source:MGI Symbol;Acc:MGI:1917655])

HSP 1 Score: 117.087 bits (292), Expect = 1.476e-32
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
             LTE++   +KEAF LFD D +G I  QEL  +  S+G++P++++L  M+ + D  + +G ++F EF+  M +  +K     E+++ AFRVFD+DGNG +   ELR  M  LGE L++ E+D MI  AD DGDG ++++EF  M ++
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGSITTQELGTVMRSLGQNPTEAELQGMVNEIDK-DGNGTVDFPEFLTMM-SRKMKDTDSEEEIREAFRVFDKDGNGFVSAAELRHVMTKLGEKLSDEEVDEMIQAADTDGDGQVNYEEFVHMLVS 148          
BLAST of Calmodulin, putative vs. UniProt/SwissProt
Match: sp|P13868|CALM1_SOLTU (Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 PE=1 SV=2)

HSP 1 Score: 128.642 bits (322), Expect = 4.199e-36
Identity = 68/147 (46.26%), Postives = 100/147 (68.03%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
             LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI++AD  +++G I+F EF+N M    +K     E++K AF+VFD+D NG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M LA
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMISEADA-DQNGTIDFPEFLNLMARK-MKDTDSEEELKEAFKVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVRMMLA 148          
BLAST of Calmodulin, putative vs. UniProt/SwissProt
Match: sp|P41041|CALM_PNECA (Calmodulin OS=Pneumocystis carinii OX=4754 PE=3 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 7.883e-36
Identity = 68/149 (45.64%), Postives = 99/149 (66.44%), Query Frame = 1
Query:  169 EYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            E NLTE++ + +KEAF LFD D +G I  +EL  +  S+G++P++++L  M+ + D  + +G I+F EF+  M    +K     E+++ AF+VFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG+I + EF KM L+
Sbjct:    4 EQNLTEEQISEFKEAFSLFDKDGDGSITTKELGIVMRSLGQNPTEAELQDMVNEVDA-DGNGTIDFPEFLAMMARK-MKDVDSEEEIREAFKVFDKDGNGIISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGVIDYSEFVKMMLS 150          
BLAST of Calmodulin, putative vs. UniProt/SwissProt
Match: sp|P24044|CALM_PLAFA (Calmodulin OS=Plasmodium falciparum OX=5833 PE=3 SV=4)

HSP 1 Score: 127.487 bits (319), Expect = 1.220e-35
Identity = 69/146 (47.26%), Postives = 98/146 (67.12%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++ + +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + DT + +G I+F EF+  M    +K     E++  AFRVFDRDG+G I  +ELR  M NLGE LT  E+D MI EAD DGDG I+++EF KM +A
Sbjct:    5 LTEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEIDT-DGNGTIDFPEFLTLMARK-LKDTDTEEELIEAFRVFDRDGDGYISADELRHVMTNLGEKLTNEEVDEMIREADIDGDGQINYEEFVKMMIA 148          
BLAST of Calmodulin, putative vs. UniProt/SwissProt
Match: sp|P62203|CALM_PLAF7 (Calmodulin OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF14_0323 PE=3 SV=2)

HSP 1 Score: 127.487 bits (319), Expect = 1.220e-35
Identity = 69/146 (47.26%), Postives = 98/146 (67.12%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++ + +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + DT + +G I+F EF+  M    +K     E++  AFRVFDRDG+G I  +ELR  M NLGE LT  E+D MI EAD DGDG I+++EF KM +A
Sbjct:    5 LTEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEIDT-DGNGTIDFPEFLTLMARK-LKDTDTEEELIEAFRVFDRDGDGYISADELRHVMTNLGEKLTNEEVDEMIREADIDGDGQINYEEFVKMMIA 148          
BLAST of Calmodulin, putative vs. UniProt/SwissProt
Match: sp|P84339|CALM_AGABI (Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2)

HSP 1 Score: 127.102 bits (318), Expect = 1.580e-35
Identity = 67/146 (45.89%), Postives = 99/146 (67.81%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            L+E++ + +KEAF LFD D +G I  +EL  +  S+G++PS ++L+ MI + D  + +G I+F EF+  M    ++     E++K AF+VFD+DGNG I   ELR  M NLGE LT++E+D MI EAD DGDG I+++EF KM L+
Sbjct:    5 LSEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPSQAELEDMINEVDA-DGNGTIDFPEFLTMMARK-MRDTDSEEEIKEAFKVFDKDGNGYISAAELRHVMTNLGEKLTDSEVDEMIREADVDGDGQINYEEFVKMMLS 148          
BLAST of Calmodulin, putative vs. TrEMBL
Match: L8GI85 (Calmodulin, putative OS=Acanthamoeba castellanii str. Neff OX=1257118 GN=ACA1_091870 PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.668e-36
Identity = 67/149 (44.97%), Postives = 101/149 (67.79%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAES 621
            NLTE++   +K+AF LFD D +G +  +EL  +  S+G  P++ +L  MIA  DT + +GQI+F EF+  M     + +   +D++AAF+VFD+DGNG I  +ELR  M NLGE L+E E+DSMI EAD +GDG + F+EF++M  +++
Sbjct:    4 NLTEEQINEFKDAFTLFDKDNDGVVTAKELSTVLKSLGHSPTEQELGEMIASVDT-DGNGQIDFSEFLTMMARRMSEVQGEDDDLRAAFKVFDKDGNGFISPQELRQVMINLGEKLSEEEIDSMIREADSNGDGQVDFEEFARMMASKA 151          
BLAST of Calmodulin, putative vs. TrEMBL
Match: C3ZEV6 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_115029 PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 5.730e-35
Identity = 68/144 (47.22%), Postives = 99/144 (68.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMF 609
            LTE++ + ++EAFELFD D NG ID  EL  +  S+G+ P++++L  MI + DT + DG I+F EF+  M    +K   + ++++ +F+VFD+DGNG I  EELR  M NLGE LT+ E+D MI EAD DGDG ++F+EF +M 
Sbjct:    5 LTEEQISEFREAFELFDKDGNGSIDAGELGTVMKSLGQKPTETELQDMINEVDT-DGDGTIDFTEFLTMM-TQKMKDMHKEDELRDSFKVFDKDGNGVISAEELRQVMTNLGEKLTDEEVDEMIREADLDGDGQVNFEEFVRMM 146          
BLAST of Calmodulin, putative vs. TrEMBL
Match: A0A0C9MHZ6 (Calmodulin OS=Mucor ambiguus OX=91626 GN=MAM1_0026c02143 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 1.130e-34
Identity = 73/146 (50.00%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LT +E   YKEAF+LFD D +G I   EL  +    G +PSD++L  M+   D  + +G I+F EF+  ++N  +K  S  +D+K AF+VFD DGNG IDREELR  M +L E+L+E ELD+MI EAD +GDG ISFDEF  M  A
Sbjct:    5 LTAEEIAEYKEAFQLFDTDGDGSITAIELGTVLRKFGMNPSDAELQDMVNDVDA-DGNGNIDFTEFLGLVKN--MKTGSEGDDLKEAFKVFDVDGNGLIDREELRKVMASLNESLSEEELDAMIKEADINGDGQISFDEFKIMMGA 147          
BLAST of Calmodulin, putative vs. TrEMBL
Match: A0A1Z5L7V3 (Uncharacterized protein (Fragment) OS=Ornithodoros moubata OX=6938 PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 1.817e-34
Identity = 69/149 (46.31%), Postives = 98/149 (65.77%), Query Frame = 1
Query:  169 EYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            EY LTE++   +KEAF LFD D +G I   EL  + HS+G+ P++++L  M+   DT + +G IEF EF+ FM +  +K     E++  AFRVFD++G+G I   ELR  M NLGE LT+ E++ MI EAD DGDGL+++DEF  +  A
Sbjct:    3 EYGLTEEQVAEFKEAFLLFDKDSDGRITASELGTVMHSLGQRPTEAELRNMVTLVDT-DGNGTIEFNEFL-FMMSKKMKETDSEEELHEAFRVFDKNGDGFISASELRHVMTNLGEKLTDEEVEDMIKEADLDGDGLVNYDEFVTILTA 149          
BLAST of Calmodulin, putative vs. TrEMBL
Match: A0A1Y1Z9W4 (Calmodulin OS=Basidiobolus meristosporus CBS 931.73 OX=1314790 GN=K493DRAFT_310319 PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 2.366e-34
Identity = 69/146 (47.26%), Postives = 99/146 (67.81%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI++ D  + +GQI+F EF+  M    +K     E++K AF+VFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG I++DEF KM ++
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMISEIDA-DGNGQIDFTEFLTLMARK-MKDTDSEEEIKEAFKVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYDEFVKMMMS 148          
BLAST of Calmodulin, putative vs. Ensembl Cavefish
Match: calm3a (calmodulin [Source:NCBI gene;Acc:103037741])

HSP 1 Score: 124.02 bits (310), Expect = 2.732e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Cavefish
Match: calm2a (calmodulin [Source:NCBI gene;Acc:103037741])

HSP 1 Score: 124.02 bits (310), Expect = 2.732e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Cavefish
Match: calm1b (calmodulin [Source:NCBI gene;Acc:103037741])

HSP 1 Score: 124.02 bits (310), Expect = 2.732e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Cavefish
Match: calm3a (calmodulin [Source:NCBI gene;Acc:103037741])

HSP 1 Score: 124.02 bits (310), Expect = 2.732e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Cavefish
Match: ENSAMXT00000033058.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001094.1:36754:37634:-1 gene:ENSAMXG00000036034.1 transcript:ENSAMXT00000033058.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 120.168 bits (300), Expect = 1.433e-33
Identity = 68/167 (40.72%), Postives = 104/167 (62.28%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS*NIYISFSAILSVFIFL 675
             LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++   + + A+   +  I  SA +SV + +
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYE--GERWSADPQVHCIIIQSAAISVLLMI 166          
BLAST of Calmodulin, putative vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 124.02 bits (310), Expect = 5.085e-36
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Sea Lamprey
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 124.02 bits (310), Expect = 5.542e-36
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006008.1 (pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-1 gene:ENSPMAG00000005430.1 transcript:ENSPMAT00000006008.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 123.635 bits (309), Expect = 8.076e-36
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITVKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Sea Lamprey
Match: si:rp71-17i16.4 (si:rp71-17i16.4 [Source:ZFIN;Acc:ZDB-GENE-090312-215])

HSP 1 Score: 92.0485 bits (227), Expect = 1.685e-23
Identity = 54/146 (36.99%), Postives = 88/146 (60.27%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFM-RNNAVKHRSRTED-MKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMF 609
            L E++   +K AF++FD D  G I   EL K+   +G+ P+  +LD +I + D  +  G I+F+EF+  M R    +   +TE+ +  AFR+ D +G+G IDR+EL+  + N GE +T+ E+D ++ + D + DG + FDEF KM 
Sbjct:   17 LNEEQIAEFKAAFDMFDADGGGDISTSELGKVMKLLGQTPTKEELDAIIEEVDE-DGSGTIDFEEFLVMMVRQMKEESAGQTEEELAEAFRILDTNGDGYIDRDELKDILLNTGENVTDLEMDELMKDGDKNCDGRLDFDEFLKMM 161          

HSP 2 Score: 57.7658 bits (138), Expect = 9.010e-11
Identity = 32/72 (44.44%), Postives = 43/72 (59.72%), Query Frame = 1
Query:  397 AVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFL 612
            A  +  +  + KAAF +FD DG G I   EL   M+ LG+T T+ ELD++I E D DG G I F+EF  M +
Sbjct:   15 AYLNEEQIAEFKAAFDMFDADGGGDISTSELGKVMKLLGQTPTKEELDAIIEEVDEDGSGTIDFEEFLVMMV 86          

HSP 3 Score: 44.2838 bits (103), Expect = 4.532e-6
Identity = 23/65 (35.38%), Postives = 39/65 (60.00%), Query Frame = 1
Query:  208 EAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAV 402
            EAF + D + +GYID+ EL  +  + GE+ +D ++D ++   D  N DG+++F EF+  M   A 
Sbjct:  103 EAFRILDTNGDGYIDRDELKDILLNTGENVTDLEMDELMKDGDK-NCDGRLDFDEFLKMMEGIAA 166          
BLAST of Calmodulin, putative vs. Ensembl Sea Lamprey
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 92.0485 bits (227), Expect = 2.880e-23
Identity = 59/140 (42.14%), Postives = 82/140 (58.57%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEF 597
            L+E++    KEAF+LFD D +G ID +EL     ++G  P   ++  MIA  D  +  G+I F +F+  M     +  SR E +KA FR+FD D  G I  + L+   + LGETLT+ EL  MI EAD DGDG I+ +EF
Sbjct:   36 LSEEQKQEIKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDK-DGSGKINFNDFLAMMTQKMSEKDSREEILKA-FRLFDDDETGKISFKNLKRVAKELGETLTDEELQEMIDEADRDGDGEINEEEF 173          

HSP 2 Score: 53.9138 bits (128), Expect = 2.699e-9
Identity = 28/70 (40.00%), Postives = 45/70 (64.29%), Query Frame = 1
Query:  415 RTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS 624
            + +++K AF +FD DG+G+ID +EL++AM  LG    + E+  MI + D DG G I+F++F  M   + S
Sbjct:   40 QKQEIKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDKDGSGKINFNDFLAMMTQKMS 109          
BLAST of Calmodulin, putative vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 98.9821 bits (245), Expect = 1.283e-26
Identity = 58/151 (38.41%), Postives = 91/151 (60.26%), Query Frame = 1
Query:  166 LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQL-DLMIAKADTLNRDG--QIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMF 609
            +  NLTE++   +KEAF LFD D NG I   EL  +  S+G  PS++++ DLM    + ++ DG  QIEF EF+  M    +K     +++  AF+VFD++G+G I   EL+  + ++GE LT+AE+D M+ E   DG G I+  +F+ + 
Sbjct:    1 MSSNLTEEQIAEFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLM----NEIDVDGNHQIEFSEFLALMSRQ-LKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDDMLREV-SDGSGEINIQQFAALL 145          
BLAST of Calmodulin, putative vs. Ensembl Yeast
Match: CDC31 (Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation [Source:SGD;Acc:S000005783])

HSP 1 Score: 72.7886 bits (177), Expect = 1.785e-16
Identity = 54/147 (36.73%), Postives = 82/147 (55.78%), Query Frame = 1
Query:  166 LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIG-EHPSDSQLDLMIAKADTLNRDGQ--IEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEF 597
            L   L E++     EAF LFD + +G++D  EL     ++G E P    LDL+    D  + +G+  +++ +F   M    +K R   +++K AF++FD D  G I  + LR   + LGETLT+ EL +MI E D DGDG I+ +EF
Sbjct:   13 LNSELLEEQKQEIYEAFSLFDMNNDGFLDYHELKVAMKALGFELPKREILDLI----DEYDSEGRHLMKYDDFYIVMGEKILK-RDPLDEIKRAFQLFDDDHTGKISIKNLRRVAKELGETLTDEELRAMIEEFDLDGDGEINENEF 154          
BLAST of Calmodulin, putative vs. Ensembl Yeast
Match: MLC1 (Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition [Source:SGD;Acc:S000003074])

HSP 1 Score: 67.781 bits (164), Expect = 8.258e-15
Identity = 44/138 (31.88%), Postives = 70/138 (50.72%), Query Frame = 1
Query:  199 AYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQ-IEFQEFVNFMRNNA----VKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEF 597
            A K+ F LFD    G I K  L     +IG +P++ QL   I  AD+  RD   +   +    +  N        +++TED   AF+VFD++  G +   +LR  +  LGE LT+AE+D ++   + D +G I + +F
Sbjct:    6 ANKDIFTLFDKKGQGAIAKDSLGDYLRAIGYNPTN-QLVQDIINADSSLRDASSLTLDQITGLIEVNEKELDATTKAKTEDFVKAFQVFDKESTGKVSVGDLRYMLTGLGEKLTDAEVDELLKGVEVDSNGEIDYKKF 142          
BLAST of Calmodulin, putative vs. Ensembl Yeast
Match: CNB1 (Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000001673])

HSP 1 Score: 54.299 bits (129), Expect = 1.069e-9
Identity = 43/146 (29.45%), Postives = 74/146 (50.68%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAME-----NLGETLTEAELDSMIHEADCDGDGLISFDEF 597
            N    E    ++ F   D D +G IDK E + +   +  +P   ++ + +  AD     G ++FQEF+  +   + +  S+ E ++ AF+++D D +G I   EL I ++     NL +   +  +D  I E D DGDG +SF+EF
Sbjct:   17 NFDRDEIERLRKRFMKLDRDSSGSIDKNEFMSI-PGVSSNPLAGRI-MEVFDADN---SGDVDFQEFITGLSIFSGRG-SKDEKLRFAFKIYDIDKDGFISNGELFIVLKIMVGSNLDDEQLQQIVDRTIVENDSDGDGRLSFEEF 156          
BLAST of Calmodulin, putative vs. Ensembl Nematostella
Match: EDO46518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPI8])

HSP 1 Score: 123.635 bits (309), Expect = 1.305e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF KM  +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVKMMTS 148          
BLAST of Calmodulin, putative vs. Ensembl Nematostella
Match: EDO46606 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPN8])

HSP 1 Score: 122.479 bits (306), Expect = 3.106e-35
Identity = 65/141 (46.10%), Postives = 94/141 (66.67%), Query Frame = 1
Query:  196 TAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAE 618
            T +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K+    E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG +++DEF KM  ++
Sbjct:    2 TEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKNTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYDEFVKMMTSK 140          
BLAST of Calmodulin, putative vs. Ensembl Nematostella
Match: EDO30674 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T043])

HSP 1 Score: 123.25 bits (308), Expect = 4.186e-35
Identity = 63/145 (43.45%), Postives = 97/145 (66.90%), Query Frame = 1
Query:  172 YNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
            + LT ++    +EAF LFD D +G I  +EL  +  S+G++PSD +L  MI + D  + +G+++F+EF+  M+   ++HR    +M+ AFRVFDR+G+GSI   ELR  M +LGE L++ E+  M+ EAD DGDG+I+F EF +M
Sbjct:   11 FELTAEQRNELQEAFSLFDKDGSGTISNEELEVVMKSLGQNPSDEELQQMIQEVDA-DGNGEVDFEEFLAMMKKQ-MQHRDAEAEMREAFRVFDRNGDGSISEWELRSVMASLGEKLSDDEIKEMMREADLDGDGVINFQEFVQM 153          
BLAST of Calmodulin, putative vs. Ensembl Nematostella
Match: EDO33580 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRU7])

HSP 1 Score: 121.709 bits (304), Expect = 8.459e-35
Identity = 63/147 (42.86%), Postives = 97/147 (65.99%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            +LTE+E   YKEAF LFD D +G +   EL  +  ++G++P+D ++  MI + D  +  G I F+EF+  M +   K +S  +++ AAF++FD+DGNGSI   EL+  +++LGE L+E E+  MI EAD DGDG ++ +EF KM +A
Sbjct:    4 DLTEEEIAEYKEAFSLFDKDGDGTVTTAELGTVMRNLGQNPTDEEIREMIKEVDE-DGSGSIGFEEFLQLM-SKKTKGKSYEDELMAAFQIFDKDGNGSITVTELKEVLDSLGEKLSEDEVGEMIKEADSDGDGTVNIEEFIKMMVA 148          
BLAST of Calmodulin, putative vs. Ensembl Nematostella
Match: EDO30675 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T044])

HSP 1 Score: 108.997 bits (271), Expect = 4.461e-30
Identity = 57/131 (43.51%), Postives = 85/131 (64.89%), Query Frame = 1
Query:  196 TAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISF 588
            +  KEAF LFD D  G I   EL  +  S+G++P++ +L  MIA+ D  + +G+I+F EF+  M    +K     E+++ AF+VFD++G+G I   EL++ M NLGE LT+ E+D MI EAD DGDG+I +
Sbjct:    2 SELKEAFALFDKDGGGSISSSELAHVMRSLGQNPTEQELKDMIAEVDQ-DDNGEIDFPEFLQ-MVAKKIKETDTEEEIQDAFKVFDKNGDGMISSSELKLVMSNLGERLTDDEVDEMIREADIDGDGMIDY 130          

HSP 2 Score: 55.8398 bits (133), Expect = 3.728e-10
Identity = 30/61 (49.18%), Postives = 38/61 (62.30%), Query Frame = 1
Query:  424 DMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
            ++K AF +FD+DG GSI   EL   M +LG+  TE EL  MI E D D +G I F EF +M
Sbjct:    3 ELKEAFALFDKDGGGSISSSELAHVMRSLGQNPTEQELKDMIAEVDQDDNGEIDFPEFLQM 63          
BLAST of Calmodulin, putative vs. Ensembl Medaka
Match: calm2a (calmodulin 2 [Source:NCBI gene;Acc:100049420])

HSP 1 Score: 124.02 bits (310), Expect = 2.715e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Medaka
Match: ENSORLT00000008599.2 (calmodulin 3 [Source:NCBI gene;Acc:100049421])

HSP 1 Score: 124.02 bits (310), Expect = 2.715e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Medaka
Match: calm1b (calmodulin [Source:NCBI gene;Acc:100049419])

HSP 1 Score: 124.02 bits (310), Expect = 2.715e-35
Identity = 66/146 (45.21%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLA 615
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calmodulin, putative vs. Ensembl Medaka
Match: ENSORLT00000022188.2 (calmodulin [Source:NCBI gene;Acc:100049422])

HSP 1 Score: 122.865 bits (307), Expect = 1.118e-34
Identity = 66/148 (44.59%), Postives = 97/148 (65.54%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAE 618
             LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF +M  A+
Sbjct:   18 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 163          
BLAST of Calmodulin, putative vs. Ensembl Medaka
Match: si:rp71-17i16.4 (troponin C, skeletal muscle [Source:NCBI gene;Acc:101171577])

HSP 1 Score: 93.9745 bits (232), Expect = 5.318e-23
Identity = 54/145 (37.24%), Postives = 86/145 (59.31%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRS--RTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
            LTE+  T +K AF++FD D  G I  +EL  +   +G++PS  +LD +I + D  +  G I+F+EF+  M     + ++    E++   FR+FD++ +G +DREE    +   GE +TE ++D M  EAD + DG + FDEF KM
Sbjct:   77 LTEEMITEFKAAFDMFDTDGGGDISTKELGTVMRMLGQNPSREELDAIIEEVDE-DGSGTIDFEEFLVMMVQQLKEDQAGKSEEELSECFRIFDKNSDGFVDREEFGDILHMTGEAVTEEDIDEMFGEADSNKDGKLDFDEFLKM 220          

HSP 2 Score: 55.4546 bits (132), Expect = 6.161e-9
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 1
Query:  430 KAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAE 618
            KAAF +FD DG G I  +EL   M  LG+  +  ELD++I E D DG G I F+EF  M + +
Sbjct:   86 KAAFDMFDTDGGGDISTKELGTVMRMLGQNPSREELDAIIEEVDEDGSGTIDFEEFLVMMVQQ 148          

HSP 3 Score: 50.447 bits (119), Expect = 3.402e-7
Identity = 22/62 (35.48%), Postives = 40/62 (64.52%), Query Frame = 1
Query:  208 EAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRN 393
            E F +FD + +G++D++E   + H  GE  ++  +D M  +AD+ N+DG+++F EF+  M N
Sbjct:  163 ECFRIFDKNSDGFVDREEFGDILHMTGEAVTEEDIDEMFGEADS-NKDGKLDFDEFLKMMEN 223          
BLAST of Calmodulin, putative vs. Planmine SMEST
Match: SMESG000040933.1 (SMESG000040933.1)

HSP 1 Score: 315.464 bits (807), Expect = 2.035e-110
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 1
Query:  166 LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS 624
            LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS
Sbjct:   19 LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS 171          
BLAST of Calmodulin, putative vs. Planmine SMEST
Match: SMESG000040933.1 (SMESG000040933.1)

HSP 1 Score: 314.309 bits (804), Expect = 4.323e-110
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 1
Query:  166 LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS 624
            LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS
Sbjct:    6 LEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS 158          
BLAST of Calmodulin, putative vs. Planmine SMEST
Match: SMESG000034095.1 (SMESG000034095.1)

HSP 1 Score: 121.324 bits (303), Expect = 1.919e-34
Identity = 65/143 (45.45%), Postives = 94/143 (65.73%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTM 145          

HSP 2 Score: 53.9138 bits (128), Expect = 6.549e-9
Identity = 26/61 (42.62%), Postives = 41/61 (67.21%), Query Frame = 1
Query:  205 KEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFM 387
            +EAF +FD D NG+I   EL  +  ++GE  +D ++D MI +AD ++ DGQ+ ++EFV  M
Sbjct:   87 REAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREAD-IDGDGQVNYEEFVTMM 146          
BLAST of Calmodulin, putative vs. Planmine SMEST
Match: SMESG000010769.1 (SMESG000010769.1)

HSP 1 Score: 121.324 bits (303), Expect = 1.919e-34
Identity = 65/143 (45.45%), Postives = 94/143 (65.73%), Query Frame = 1
Query:  178 LTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKM 606
            LTE++   +KEAF LFD D +G I  +EL  +  S+G++P++++L  MI + D  + +G I+F EF+  M    +K     E+++ AFRVFD+DGNG I   ELR  M NLGE LT+ E+D MI EAD DGDG ++++EF  M
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA-DGNGTIDFPEFLTMMARK-MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTM 145          

HSP 2 Score: 53.9138 bits (128), Expect = 6.549e-9
Identity = 26/61 (42.62%), Postives = 41/61 (67.21%), Query Frame = 1
Query:  205 KEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFM 387
            +EAF +FD D NG+I   EL  +  ++GE  +D ++D MI +AD ++ DGQ+ ++EFV  M
Sbjct:   87 REAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREAD-IDGDGQVNYEEFVTMM 146          
BLAST of Calmodulin, putative vs. Planmine SMEST
Match: SMESG000078272.1 (SMESG000078272.1)

HSP 1 Score: 114.39 bits (285), Expect = 1.760e-31
Identity = 56/150 (37.33%), Postives = 99/150 (66.00%), Query Frame = 1
Query:  175 NLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPSDSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVFDRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFSKMFLAESS 624
            ++T ++    +E F LFD D  G I  QEL  +  ++G++P++++L  MI++ D ++ +GQI+F+EF   M N  +K      D+K  F+VFDR+G+G + R EL+I M N+GE LT++E++ +++  D +GDG+I F+EF+ + +  +S
Sbjct:   12 DMTPEQIAEMQEIFSLFDKDGGGTITTQELGAVMRALGQNPTEAELQDMISEID-IDGNGQIDFEEFCILM-NRRIKDSDLETDLKDVFKVFDRNGDGKVTRNELKIVMCNIGEVLTDSEIEEIMNLVDINGDGIIDFEEFNNLMINNAS 159          
The following BLAST results are available for this feature:
BLAST of Calmodulin, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALM25.067e-3545.21calmodulin 2 [Source:HGNC Symbol;Acc:HGNC:1445][more]
CALM25.067e-3545.21calmodulin 2 [Source:HGNC Symbol;Acc:HGNC:1445][more]
CALM15.067e-3545.21calmodulin 1 [Source:HGNC Symbol;Acc:HGNC:1442][more]
CALM35.067e-3545.21calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449][more]
CALM35.067e-3545.21calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449][more]
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BLAST of Calmodulin, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cmd-11.831e-3445.45Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
cal-19.865e-3041.67Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cal-11.812e-2941.67Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cal-23.373e-2840.41CALmodulin related genes; Calmodulin-like protein ... [more]
cal-31.543e-2540.69CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
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BLAST of Calmodulin, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cam1.956e-3444.52gene:FBgn0000253 transcript:FBtr0304964[more]
Cam1.956e-3444.52gene:FBgn0000253 transcript:FBtr0088001[more]
Cam1.956e-3444.52gene:FBgn0000253 transcript:FBtr0304963[more]
Cam1.956e-3444.52gene:FBgn0000253 transcript:FBtr0088002[more]
Cam1.956e-3444.52gene:FBgn0000253 transcript:FBtr0345018[more]
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BLAST of Calmodulin, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calm2a2.958e-3545.21calmodulin 2a (phosphorylase kinase, delta) [Sourc... [more]
calm3a3.224e-3545.21calmodulin 3a (phosphorylase kinase, delta) [Sourc... [more]
calm3b3.224e-3545.21calmodulin 3b (phosphorylase kinase, delta) [Sourc... [more]
calm1b3.224e-3545.21calmodulin 1b [Source:ZFIN;Acc:ZDB-GENE-030804-2][more]
calm1b3.224e-3545.21calmodulin 1b [Source:ZFIN;Acc:ZDB-GENE-030804-2][more]
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BLAST of Calmodulin, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALM24.594e-3545.21calmodulin 1 (phosphorylase kinase, delta) [Source... [more]
CALM24.594e-3545.21calmodulin 1 (phosphorylase kinase, delta) [Source... [more]
calm24.594e-3545.21calmodulin 2 (phosphorylase kinase, delta) [Source... [more]
calm24.594e-3545.21calmodulin 2 (phosphorylase kinase, delta) [Source... [more]
CALM23.215e-3345.00calmodulin 1 (phosphorylase kinase, delta) [Source... [more]
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BLAST of Calmodulin, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Calm33.479e-3545.21calmodulin 3 [Source:MGI Symbol;Acc:MGI:103249][more]
Calm13.479e-3545.21calmodulin 1 [Source:MGI Symbol;Acc:MGI:88251][more]
Calm23.479e-3545.21calmodulin 2 [Source:MGI Symbol;Acc:MGI:103250][more]
Calm11.186e-3444.59calmodulin 1 [Source:MGI Symbol;Acc:MGI:88251][more]
Calml31.476e-3240.82calmodulin-like 3 [Source:MGI Symbol;Acc:MGI:19176... [more]
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BLAST of Calmodulin, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P13868|CALM1_SOLTU4.199e-3646.26Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 ... [more]
sp|P41041|CALM_PNECA7.883e-3645.64Calmodulin OS=Pneumocystis carinii OX=4754 PE=3 SV... [more]
sp|P24044|CALM_PLAFA1.220e-3547.26Calmodulin OS=Plasmodium falciparum OX=5833 PE=3 S... [more]
sp|P62203|CALM_PLAF71.220e-3547.26Calmodulin OS=Plasmodium falciparum (isolate 3D7) ... [more]
sp|P84339|CALM_AGABI1.580e-3545.89Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2[more]
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BLAST of Calmodulin, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
L8GI853.668e-3644.97Calmodulin, putative OS=Acanthamoeba castellanii s... [more]
C3ZEV65.730e-3547.22Uncharacterized protein OS=Branchiostoma floridae ... [more]
A0A0C9MHZ61.130e-3450.00Calmodulin OS=Mucor ambiguus OX=91626 GN=MAM1_0026... [more]
A0A1Z5L7V31.817e-3446.31Uncharacterized protein (Fragment) OS=Ornithodoros... [more]
A0A1Y1Z9W42.366e-3447.26Calmodulin OS=Basidiobolus meristosporus CBS 931.7... [more]
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BLAST of Calmodulin, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calm3a2.732e-3545.21calmodulin [Source:NCBI gene;Acc:103037741][more]
calm2a2.732e-3545.21calmodulin [Source:NCBI gene;Acc:103037741][more]
calm1b2.732e-3545.21calmodulin [Source:NCBI gene;Acc:103037741][more]
calm3a2.732e-3545.21calmodulin [Source:NCBI gene;Acc:103037741][more]
ENSAMXT00000033058.11.433e-3340.72pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of Calmodulin, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALML35.085e-3645.21calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145... [more]
CALM35.542e-3645.21calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449][more]
ENSPMAT00000006008.18.076e-3645.21pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-... [more]
si:rp71-17i16.41.685e-2336.99si:rp71-17i16.4 [Source:ZFIN;Acc:ZDB-GENE-090312-2... [more]
CETN12.880e-2342.14centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866][more]
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BLAST of Calmodulin, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
CMD11.283e-2638.41Calmodulin; Ca2+ binding protein that regulates Ca... [more]
CDC311.785e-1636.73Calcium-binding component of the spindle pole body... [more]
MLC18.258e-1531.88Essential light chain for Myo1p; light chain for M... [more]
CNB11.069e-929.45Calcineurin B; regulatory subunit of calcineurin, ... [more]
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BLAST of Calmodulin, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO465181.305e-3545.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO466063.106e-3546.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO306744.186e-3543.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO335808.459e-3542.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO306754.461e-3043.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calmodulin, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calm2a2.715e-3545.21calmodulin 2 [Source:NCBI gene;Acc:100049420][more]
ENSORLT00000008599.22.715e-3545.21calmodulin 3 [Source:NCBI gene;Acc:100049421][more]
calm1b2.715e-3545.21calmodulin [Source:NCBI gene;Acc:100049419][more]
ENSORLT00000022188.21.118e-3444.59calmodulin [Source:NCBI gene;Acc:100049422][more]
si:rp71-17i16.45.318e-2337.24troponin C, skeletal muscle [Source:NCBI gene;Acc:... [more]
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BLAST of Calmodulin, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000040933.12.035e-110100.00SMESG000040933.1[more]
SMESG000040933.14.323e-110100.00SMESG000040933.1[more]
SMESG000034095.11.919e-3445.45SMESG000034095.1[more]
SMESG000010769.11.919e-3445.45SMESG000010769.1[more]
SMESG000078272.11.760e-3137.33SMESG000078272.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021154 ID=SMED30021154|Name=Calmodulin, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=754bp
GTCTGTTACAGATTATCATTTGATAATATTAATTGAATCTCAATTCTGAT
CTGTCACAATTAATTCATTGCTTGTTTTATTATTCTTCATTTTGCTGCTA
ATATCAACCAATAAATTGAATCAAGAAACAAAAAGAGAAGCAAAAAAAAT
ATGGAGAATAAAAAATTGGAATACAATTTAACAGAAAAAGAATACACGGC
TTACAAAGAAGCTTTTGAACTTTTCGACAACGACCGAAATGGATACATCG
ACAAACAAGAACTATTGAAAATGTGCCATAGCATCGGAGAACATCCGTCA
GATTCTCAGTTGGATTTGATGATTGCTAAAGCCGATACGTTGAATCGGGA
TGGCCAAATAGAATTTCAAGAGTTCGTGAATTTCATGAGAAACAATGCCG
TTAAACATAGATCCCGCACTGAAGACATGAAGGCTGCTTTTCGAGTCTTT
GACAGAGATGGCAATGGAAGCATCGATAGAGAGGAATTGAGAATTGCGAT
GGAGAATCTTGGAGAAACTTTAACGGAAGCAGAATTGGACAGCATGATCC
ACGAAGCCGATTGCGACGGTGACGGTTTAATATCTTTTGACGAGTTTTCC
AAAATGTTTTTAGCGGAATCATCTTGAAATATCTACATCTCTTTCTCTGC
GATTTTATCAGTTTTTATATTTTTATAGAAGAAATAATTATCATAAAACT
ATTGCATTACAAATAATATATTTTATAATAAAGGTGTTTTTATCTCTAAA
AAAA
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protein sequence of SMED30021154-orf-1

>SMED30021154-orf-1 ID=SMED30021154-orf-1|Name=SMED30021154-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=159bp
MENKKLEYNLTEKEYTAYKEAFELFDNDRNGYIDKQELLKMCHSIGEHPS
DSQLDLMIAKADTLNRDGQIEFQEFVNFMRNNAVKHRSRTEDMKAAFRVF
DRDGNGSIDREELRIAMENLGETLTEAELDSMIHEADCDGDGLISFDEFS
KMFLAESS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016459myosin complex
Vocabulary: biological process
TermDefinition
GO:0019722calcium-mediated signaling
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000067goblet cell
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0005515protein binding
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1..21
NoneNo IPR availableCOILSCoilCoilcoord: 110..130
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..84
e-value: 2.2E-24
score: 87.5
coord: 85..158
e-value: 3.0E-26
score: 93.5
NoneNo IPR availablePANTHERPTHR23050:SF376CALMODULIN-2-RELATEDcoord: 10..156
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 10..156
IPR002048EF-hand domainSMARTSM00054efh_1coord: 17..45
e-value: 3.8E-6
score: 36.4
coord: 92..120
e-value: 1.6E-6
score: 37.7
coord: 53..82
e-value: 0.42
score: 19.7
coord: 128..156
e-value: 1.5E-4
score: 31.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 91..153
e-value: 2.0E-15
score: 57.0
coord: 18..80
e-value: 1.7E-14
score: 54.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 49..85
score: 9.018
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 124..158
score: 10.775
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 88..123
score: 16.048
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 13..48
score: 14.43
IPR002048EF-hand domainCDDcd00051EFhcoord: 92..153
e-value: 2.35604E-20
score: 77.2029
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 26..38
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 137..149
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 101..113
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 8..155