Checkpoint protein

Overview
NameCheckpoint protein
Smed IDSMED30021128
Length (bp)782
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Checkpoint protein (SMED30021128) t-SNE clustered cells

Violin plots show distribution of expression levels for Checkpoint protein (SMED30021128) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Checkpoint protein (SMED30021128) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Checkpoint protein (SMED30021128) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021128

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30021128h1SMcG0018644 SmedASXL_008811SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30021128h1SMcG0018644 dd_Smed_v6_15678_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30021128h1SMcG0018644 dd_Smed_v4_15678_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Checkpoint protein vs. Ensembl Human
Match: HUS1 (HUS1 checkpoint clamp component [Source:HGNC Symbol;Acc:HGNC:5309])

HSP 1 Score: 124.79 bits (312), Expect = 8.535e-34
Identity = 63/175 (36.00%), Postives = 108/175 (61.71%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D   + HF  I   IA L+K   LRIS   +  I+ +++  G    WC+++ ++ F E  MEG++ ENNEI LE+ S+ L ++L+     R++KIKL       PCL++ +E  S    +R VTH++P++++P ++W + + +P + + DVSIYLP LK  +++++KMKN++  L
Sbjct:    9 DGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHF--PCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKD-LQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHL 180
[Close]
BLAST of Checkpoint protein vs. Ensembl Human
Match: HUS1 (HUS1 checkpoint clamp component [Source:HGNC Symbol;Acc:HGNC:5309])

HSP 1 Score: 118.627 bits (296), Expect = 2.093e-32
Identity = 59/161 (36.65%), Postives = 103/161 (63.98%), Query Frame = 2 
Query:  125 IAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            IA L+K   LRIS   +  I+ +++  G    WC+++ ++ F E  MEG++ ENNEI LE+ S+ L ++L+     R++KIKL       PCL++ +E  S    +R VTH++P++++P ++W + + +P + + DVSIYLP LK  +++++KMKN++  L
Sbjct:    2 IAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHF--PCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKD-LQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHL 159
[Close]
BLAST of Checkpoint protein vs. Ensembl Human
Match: HUS1 (HUS1 checkpoint clamp component [Source:HGNC Symbol;Acc:HGNC:5309])

HSP 1 Score: 119.013 bits (297), Expect = 1.113e-31
Identity = 59/161 (36.65%), Postives = 103/161 (63.98%), Query Frame = 2 
Query:  125 IAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            IA L+K   LRIS   +  I+ +++  G    WC+++ ++ F E  MEG++ ENNEI LE+ S+ L ++L+     R++KIKL       PCL++ +E  S    +R VTH++P++++P ++W + + +P + + DVSIYLP LK  +++++KMKN++  L
Sbjct:    2 IAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHF--PCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKD-LQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHL 159
[Close]
BLAST of Checkpoint protein vs. Ensembl Human
Match: HUS1 (HUS1 checkpoint clamp component [Source:HGNC Symbol;Acc:HGNC:5309])

HSP 1 Score: 119.013 bits (297), Expect = 1.113e-31
Identity = 59/161 (36.65%), Postives = 103/161 (63.98%), Query Frame = 2 
Query:  125 IAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            IA L+K   LRIS   +  I+ +++  G    WC+++ ++ F E  MEG++ ENNEI LE+ S+ L ++L+     R++KIKL       PCL++ +E  S    +R VTH++P++++P ++W + + +P + + DVSIYLP LK  +++++KMKN++  L
Sbjct:    2 IAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHF--PCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKD-LQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHL 159
[Close]
BLAST of Checkpoint protein vs. Ensembl Human
Match: HUS1 (HUS1 checkpoint clamp component [Source:HGNC Symbol;Acc:HGNC:5309])

HSP 1 Score: 104.76 bits (260), Expect = 1.583e-27
Identity = 54/147 (36.73%), Postives = 91/147 (61.90%), Query Frame = 2 
Query:  125 IAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVF 562
            IA L+K   LRIS   +  I+ +++  G    WC+++ ++ F E  MEG++ ENNEI LE+ S+ L ++L+     R++KIKL       PCL++ +E  S    +R VTH++P++++P ++W + + +P + + DVSIYLP LK  
Sbjct:    2 IAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHF--PCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKD-LQEPVVPDPDVSIYLPVLKTM 145
[Close]
BLAST of Checkpoint protein vs. Ensembl Celegans
Match: hus-1 (Checkpoint protein [Source:UniProtKB/TrEMBL;Acc:G5EFI9])

HSP 1 Score: 62.003 bits (149), Expect = 4.316e-11
Identity = 53/177 (29.94%), Postives = 87/177 (49.15%), Query Frame = 2 
Query:   80 RDIGTIQHFLNILQGIAYLSKN-FVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFEN----AMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEY-DVSIYLPNLKVFRTILDKMKNL 592
            +D   I  F+ IL  ++ LSK    ++I    +  I    +  G S F   I Y S  F       M   N+E N I  E+  D L++ L     C  +K+KL K++ ++P LS+E+ ++ E D    V+H++P+ I+  + WN Y  +P I +  +SI +P  K+    L   KN+
Sbjct:    8 QDTAAIDVFIKILTSVSKLSKKRCCVKIEKNALNFICCSGLHDGGSWFSLSIPYSSQIFRKFDMVGMNPRNEEQNLIYFELEIDSLVRVL-PGGHCY-LKLKLSKSQKDEPMLSVEV-RNPEAD---IVSHQIPITIILSKYWNSY-SRPSIGHRKMSISMPPPKMMSRFLHVFKNM 177
[Close]
BLAST of Checkpoint protein vs. Ensembl Fly
Match: Hus1-like (gene:FBgn0026417 transcript:FBtr0334904)

HSP 1 Score: 79.7221 bits (195), Expect = 2.713e-17
Identity = 54/183 (29.51%), Postives = 98/183 (53.55%), Query Frame = 2 
Query:   68 RMMSRDIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINE-RVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLR--QCATCRSVKIKLMKNRVNDPCLS-IEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            R + +D   ++ F  I+  +  L+K+ V+ + +  M+ I+NE +  A S L W  I  +  F E  ME  + +   I+L V S  L ++L   +     S K+KL   R+  PC+S I    +S   + R V H+VPV I+P   W+ Y++       +++ LP+L++ ++++DK+KN++  L
Sbjct:    4 RALMQDPLYMKEFQAIVATLTKLAKDCVMILGSRQMHFIVNEDQSSAASPLVWAGITAEEYFPEYRMEAAHPDQEYIVLGVSSANLGRALSVLRGGGVNSCKLKL--QRIQFPCISVIASVLTSSSTEAREVVHDVPVTIIPGSDWSAYVVPRVPNSQLALGLPSLRLLKSLIDKLKNISPSL 184
[Close]
BLAST of Checkpoint protein vs. Ensembl Fly
Match: Hus1-like (gene:FBgn0026417 transcript:FBtr0078876)

HSP 1 Score: 79.7221 bits (195), Expect = 2.713e-17
Identity = 54/183 (29.51%), Postives = 98/183 (53.55%), Query Frame = 2 
Query:   68 RMMSRDIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINE-RVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLR--QCATCRSVKIKLMKNRVNDPCLS-IEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            R + +D   ++ F  I+  +  L+K+ V+ + +  M+ I+NE +  A S L W  I  +  F E  ME  + +   I+L V S  L ++L   +     S K+KL   R+  PC+S I    +S   + R V H+VPV I+P   W+ Y++       +++ LP+L++ ++++DK+KN++  L
Sbjct:    4 RALMQDPLYMKEFQAIVATLTKLAKDCVMILGSRQMHFIVNEDQSSAASPLVWAGITAEEYFPEYRMEAAHPDQEYIVLGVSSANLGRALSVLRGGGVNSCKLKL--QRIQFPCISVIASVLTSSSTEAREVVHDVPVTIIPGSDWSAYVVPRVPNSQLALGLPSLRLLKSLIDKLKNISPSL 184
[Close]
BLAST of Checkpoint protein vs. Ensembl Zebrafish
Match: HUS1B (zgc:162895 [Source:NCBI gene;Acc:100037341])

HSP 1 Score: 134.42 bits (337), Expect = 1.529e-37
Identity = 65/175 (37.14%), Postives = 112/175 (64.00%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+G + HF  ++  ++ L+   VLR++  ++Y +++ RV  G    WC++   + F E  +EG++ + NEI LEV  + L ++L+     +SVKIKL K     PCL++  E  S    +R VTH++PV ++P R+W+++  +PQ+ E+DVSIYLP LK  ++++D+MKNL+ +L
Sbjct:    9 DVGCLNHFTRVVXXVSXLTCACVLRLTCDHLYFVLSGRVATGGVSMWCELLQVNFFDEFQLEGVSADANEIFLEVAPENLSRALKTAQNAKSVKIKLTKKSC--PCLTLAAELPSLSSLSRVVTHDLPVDVIPRRLWHDF-REPQMPEFDVSIYLPPLKTMKSVVDRMKNLSNYL 180
[Close]
BLAST of Checkpoint protein vs. Ensembl Xenopus
Match: hus1 (HUS1 checkpoint clamp component [Source:NCBI gene;Acc:448560])

HSP 1 Score: 129.798 bits (325), Expect = 1.089e-35
Identity = 61/174 (35.06%), Postives = 106/174 (60.92%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+  + HF  ++  I+ L+K   LR++   +Y I+ ++V  G    WC++   + F E  MEG+  E NEI LE++ + L ++L+     +SVK+KL       PCL++ +E  S    +R VTH++PV ++P R+WN++      E+DVSIYLP LK  ++++++MKNL+ ++
Sbjct:    9 DVSCLNHFTRVINTISKLTKTCTLRLTVNNLYFILTDKVANGGVSMWCELSQGNFFDEYQMEGVCMEQNEIFLELIPENLSRALKTAQNAKSVKVKLTNKHC--PCLTVALELPSLSSSSRIVTHDIPVSVIPRRLWNDFKEPSVPEFDVSIYLPALKTMKSVVERMKNLSNYI 180
[Close]
BLAST of Checkpoint protein vs. Ensembl Mouse
Match: Hus1 (HUS1 checkpoint clamp component [Source:MGI Symbol;Acc:MGI:1277962])

HSP 1 Score: 107.842 bits (268), Expect = 1.659e-27
Identity = 61/176 (34.66%), Postives = 108/176 (61.36%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLS-IEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+  + HF  +   IA L+K   LRIS   +  I+ +++ +G    WC+++ ++ F E  MEG+++ENNEI LE+ S+ L ++L+     R++KIKL       PCL+     Q S    +R V H++P++++P R+W + + +P I + DVSI LP LK+ +++++KM+N++  L
Sbjct:    9 DLACLNHFTRVSNMIAKLAKTCTLRISPEKLNFILCDKLASGGVSMWCELEQENFFSEFQMEGVSEENNEIYLELTSENLSRALKTAQNSRALKIKLTNKHF--PCLTVSVELQVSSSSSSRIVVHDIPIKVLPRRLWKD-LQEPSIPDCDVSICLPALKMMKSVVEKMRNISNQL 181
[Close]
BLAST of Checkpoint protein vs. Ensembl Mouse
Match: Hus1 (HUS1 checkpoint clamp component [Source:MGI Symbol;Acc:MGI:1277962])

HSP 1 Score: 107.842 bits (268), Expect = 1.659e-27
Identity = 61/176 (34.66%), Postives = 108/176 (61.36%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLS-IEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+  + HF  +   IA L+K   LRIS   +  I+ +++ +G    WC+++ ++ F E  MEG+++ENNEI LE+ S+ L ++L+     R++KIKL       PCL+     Q S    +R V H++P++++P R+W + + +P I + DVSI LP LK+ +++++KM+N++  L
Sbjct:    9 DLACLNHFTRVSNMIAKLAKTCTLRISPEKLNFILCDKLASGGVSMWCELEQENFFSEFQMEGVSEENNEIYLELTSENLSRALKTAQNSRALKIKLTNKHF--PCLTVSVELQVSSSSSSRIVVHDIPIKVLPRRLWKD-LQEPSIPDCDVSICLPALKMMKSVVEKMRNISNQL 181
[Close]
BLAST of Checkpoint protein vs. Ensembl Mouse
Match: Hus1b (HUS1 checkpoint clamp component B [Source:MGI Symbol;Acc:MGI:2671003])

HSP 1 Score: 96.2857 bits (238), Expect = 3.567e-23
Identity = 54/168 (32.14%), Postives = 98/168 (58.33%), Query Frame = 2 
Query:   95 IQHFLNILQGIAYLSKNFVLRISNTYMY-CIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIE-YDVSIYLPNLKVFRTILDKMKNL 592
            I+ F+ +   +A L+K  VLR+    +Y C +      G +  W ++  + +F    MEG +QE NEI LE++S++L ++++      S+K++L   +   PCL++ +E +S    TR V H++PVR++P R W +   +P +   DVS+YLP LK  + ++++M N+
Sbjct:   13 IELFIQVSSTVAKLAKVCVLRVCPDRLYFCPMG---LLGEAQLWGEM-RRDVFHHFCMEGASQEFNEICLELMSEHLARAVKNAGNASSLKLQLTNKQ--RPCLTLVVELASCPGHTRAVVHDLPVRVLPRRRWKD-CTEPHVRGSDVSVYLPALKTLKNMVERMANV 173
[Close]
BLAST of Checkpoint protein vs. UniProt/SwissProt
Match: sp|O60921|HUS1_HUMAN (Checkpoint protein HUS1 OS=Homo sapiens OX=9606 GN=HUS1 PE=1 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 4.099e-33
Identity = 63/175 (36.00%), Postives = 108/175 (61.71%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D   + HF  I   IA L+K   LRIS   +  I+ +++  G    WC+++ ++ F E  MEG++ ENNEI LE+ S+ L ++L+     R++KIKL       PCL++ +E  S    +R VTH++P++++P ++W + + +P + + DVSIYLP LK  +++++KMKN++  L
Sbjct:    9 DGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHF--PCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKD-LQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHL 180
[Close]
BLAST of Checkpoint protein vs. UniProt/SwissProt
Match: sp|Q8BQY8|HUS1_MOUSE (Checkpoint protein HUS1 OS=Mus musculus OX=10090 GN=Hus1 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.054e-28
Identity = 61/175 (34.86%), Postives = 110/175 (62.86%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+  + HF  +   IA L+K   LRIS   +  I+ +++ +G    WC+++ ++ F E  MEG+++ENNEI LE+ S+ L ++L+     R++KIKL       PCL++ +E  S    +R V H++P++++P R+W + + +P I + DVSI LP LK+ +++++KM+N++  L
Sbjct:    9 DLACLNHFTRVSNMIAKLAKTCTLRISPEKLNFILCDKLASGGVSMWCELEQENFFSEFQMEGVSEENNEIYLELTSENLSRALKTAQNSRALKIKLTNKHF--PCLTVSVELVSSSSSSRIVVHDIPIKVLPRRLWKD-LQEPSIPDCDVSICLPALKMMKSVVEKMRNISNQL 180
[Close]
BLAST of Checkpoint protein vs. UniProt/SwissProt
Match: sp|Q8K572|HUS1B_MOUSE (Checkpoint protein HUS1B OS=Mus musculus OX=10090 GN=Hus1b PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 2.495e-22
Identity = 54/168 (32.14%), Postives = 98/168 (58.33%), Query Frame = 2 
Query:   95 IQHFLNILQGIAYLSKNFVLRISNTYMY-CIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIE-YDVSIYLPNLKVFRTILDKMKNL 592
            I+ F+ +   +A L+K  VLR+    +Y C +      G +  W ++  + +F    MEG +QE NEI LE++S++L ++++      S+K++L   +   PCL++ +E +S    TR V H++PVR++P R W +   +P +   DVS+YLP LK  + ++++M N+
Sbjct:   13 IELFIQVSSTVAKLAKVCVLRVCPDRLYFCPMG---LLGEAQLWGEM-RRDVFHHFCMEGASQEFNEICLELMSEHLARAVKNAGNASSLKLQLTNKQ--RPCLTLVVELASCPGHTRAVVHDLPVRVLPRRRWKD-CTEPHVRGSDVSVYLPALKTLKNMVERMANV 173
[Close]
BLAST of Checkpoint protein vs. UniProt/SwissProt
Match: sp|Q8NHY5|HUS1B_HUMAN (Checkpoint protein HUS1B OS=Homo sapiens OX=9606 GN=HUS1B PE=1 SV=2)

HSP 1 Score: 80.8777 bits (198), Expect = 1.208e-16
Identity = 47/168 (27.98%), Postives = 93/168 (55.36%), Query Frame = 2 
Query:   89 GTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNL 592
            G ++ F+++   +A L+K  VLR+    +            +  WC++  +  F +  MEG++++ +EI LE+ +++L ++ R  A   S+K++L   R   P L++ +E  S + + R V H++PVR++P R+W + +       D SI LP  +  R+I+++M N+
Sbjct:   11 GCLELFIHVSGTVARLAKVCVLRVRPDSLCFGPAGSGGLHEARLWCEV-RQGAFQQFRMEGVSEDLDEIHLELTAEHLSRAARSAAGASSLKLQLTHKR--RPSLTVAVELVSSLGRARSVVHDLPVRVLPRRVWRDCLPPSLRASDASIRLPRWRTLRSIVERMANV 175
[Close]
BLAST of Checkpoint protein vs. UniProt/SwissProt
Match: sp|P78955|HUS1_SCHPO (Checkpoint protein hus1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=hus1 PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.993e-7
Identity = 38/126 (30.16%), Postives = 63/126 (50.00%), Query Frame = 2 
Query:  221 WCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLT 595
            W  ++ +++F +  ++     +N I LEV  D   K+LR  A      ++L K   N P LS+    S     +  VTH +PVR++  + +   I +P   E D  I+LP L   R ++DK K+L+
Sbjct:   50 WSVLEVETIFEDYVVQS--NADNVINLEVPIDNFYKALRSAANASDSTVRLSKKN-NQPLLSLSTTWSGRAFGSNIVTHNIPVRVLS-QSYVSVIKEPTAPEPDCHIFLPQLNFLRHVVDKYKSLS 171
[Close]
BLAST of Checkpoint protein vs. TrEMBL
Match: A0A1S3I6X9 (Checkpoint protein OS=Lingula unguis OX=7574 GN=LOC106161247 PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.749e-46
Identity = 79/181 (43.65%), Postives = 119/181 (65.75%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFLRQIFNDS 625
            D+G IQHF  ++  +A L+KN  +RI+++ +Y I++ER+  G    WC++   + F E AM+GL++E NEI LEVV D ++K L+   T +SVKIKL K   + PCL+ E+E  S    +R VTH+VPV ++P R+W+E+      ++DVSIY+P+LKV R + DKMKNL  ++    N S
Sbjct:    9 DVGCIQHFTRVISMVAKLAKNCTVRITSSKIYFILSERLVNGGVNIWCELSQTNFFSEYAMDGLSEEYNEIFLEVVPDNMVKCLKTAQTAKSVKIKLTKK--HSPCLTFEVELPSLTGHSRVVTHDVPVNVIPRRLWDEFEEPEMPDFDVSIYMPSLKVLRNVTDKMKNLNNYVTIAANGS 187
[Close]
BLAST of Checkpoint protein vs. TrEMBL
Match: A0A1S3H1U6 (Checkpoint protein OS=Lingula unguis OX=7574 GN=LOC106151282 PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.568e-45
Identity = 78/181 (43.09%), Postives = 118/181 (65.19%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFLRQIFNDS 625
            D+G IQHF  ++  +A L+KN  +RI+ + +Y I++ER+  G    WC++   + F E AM+GL+++ NEI LEVV D ++K L+   T +SVKIKL K   + PCL+ E+E  S    +R VTH+VPV ++P R+W+E+      ++DVSIY+P+LKV R + DKMKNL  ++    N S
Sbjct:    9 DVGCIQHFTRVISMVAKLAKNCTVRITASKIYFILSERLVNGGVNIWCELSQTNFFSEYAMDGLSEDYNEIFLEVVPDNMVKCLKTAQTAKSVKIKLTKK--HSPCLTFEVELPSLTGHSRVVTHDVPVNVIPRRLWDEFEEPEMPDFDVSIYMPSLKVLRNVTDKMKNLNNYVTIAANGS 187
[Close]
BLAST of Checkpoint protein vs. TrEMBL
Match: A0A087TQI7 (Checkpoint protein HUS1 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_23681 PE=4 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 6.406e-45
Identity = 79/170 (46.47%), Postives = 113/170 (66.47%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNL 592
            +I +IQHF  ILQ +A L+K  VLRI  T+MY I NER+ AG S  WC+I     F E  MEGL++E+NEI LE   D L  + R     +SVK+KL K  V  PCL++E+E  S    +RFV H+VP+ ++P R+W ++     IE+DVS+Y+P+LKV R+++++MKN+
Sbjct:    9 EISSIQHFTKILQTVAKLTKVGVLRIVPTHMYFIFNERITAGGSSLWCEIPQDHYFCEFNMEGLSEEHNEIYLEFQLDNLATAFRSAQAAKSVKLKLTKKHV--PCLTLEVELPSLHSHSRFVVHDVPILVIPRRLWEQFQEPSMIEFDVSLYMPSLKVVRSVVERMKNI 176
[Close]
BLAST of Checkpoint protein vs. TrEMBL
Match: R7UB68 (Checkpoint protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_167069 PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 3.114e-41
Identity = 72/182 (39.56%), Postives = 120/182 (65.93%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQI-EYDVSIYLPNLKVFRTILDKMKNLTGFLRQIFNDS 625
            DIG IQHF  ++  I+ L K+  LRI+   +Y I++ER+  G +  WCQ+     F E  ++G++ E NEI LEV  D ++++LR     +SVKIKL K  +  PCL++E+E  +    +R VTH+VPV ++P R+W+E+  +P++ ++DV++++P +K+ R ++D+MKNL+ FL    N+S
Sbjct:    9 DIGCIQHFTRVVTTISKLVKSCTLRITTDTLYFILSERMVTGGTGIWCQVSQNHFFDEFNLDGVSAEANEIYLEVNPDLMVRALRTAQNAKSVKIKLTKKHM--PCLTLEVELPTLAAHSRLVTHDVPVNVIPRRLWDEF-EEPELPDFDVTLFMPCVKILRNVVDRMKNLSNFLTISANNS 187
[Close]
BLAST of Checkpoint protein vs. TrEMBL
Match: A0A4Y2GWM0 (Checkpoint protein OS=Araneus ventricosus OX=182803 GN=HUS1 PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.073e-38
Identity = 74/174 (42.53%), Postives = 108/174 (62.07%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            +I +IQHF  IL  I  L+K  VLRI  +++Y   NER+ AG S  W +I     F E  MEGL+ E NEI LE   D LI + +     +S+K+KL K  V  PCL++E+E  S    +R V H+VPV ++P R+W ++     I++DVSIY+P LKV R+++++MKN+  F+
Sbjct:    3 EISSIQHFTRILNTIGKLTKIAVLRIIPSHLYFTFNERITAGGSSLWSEIPQDHYFCEFNMEGLSSEYNEIYLEFQIDNLITAFKSAQAAKSIKLKLTKKHV--PCLTLEVELPSLHSNSRCVVHDVPVLVIPRRLWEQFQEPSMIQFDVSIYMPPLKVVRSVVERMKNIGTFM 174
[Close]
BLAST of Checkpoint protein vs. Ensembl Cavefish
Match: zgc:162895 (HUS1 checkpoint clamp component [Source:NCBI gene;Acc:103033643])

HSP 1 Score: 134.035 bits (336), Expect = 1.874e-37
Identity = 66/179 (36.87%), Postives = 108/179 (60.34%), Query Frame = 2 
Query:   68 RMMSRDIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            R    D+G + HF  ++  I  L+K  VLR++   MY +++ +V  G    WC++   + F E  +EG++ + NEI LEV  + L ++L+     +SVKIKL K   + PCL++  E  S     R VTH++PV I+P R+W+++      ++DVSIYLP LK  ++++D+MKNL+ +L
Sbjct:    4 RAKINDVGCLNHFTRVVNTITKLTKTCVLRLTVDNMYFVLSGKVANGGVSMWCELLQVNFFEEYQLEGVSADANEICLEVAPENLSRALKTAQNAKSVKIKLTKK--HGPCLTLAAELPSLSSINRIVTHDIPVDIIPKRLWHDFKEPTVPDFDVSIYLPPLKTMKSVVDRMKNLSNYL 180
[Close]
BLAST of Checkpoint protein vs. Ensembl Sea Lamprey
Match: zgc:162895 (pep scaffold:Pmarinus_7.0:GL478384:20841:26634:-1 gene:ENSPMAG00000004430.1 transcript:ENSPMAT00000004883.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:zgc:162895)

HSP 1 Score: 129.028 bits (323), Expect = 3.015e-36
Identity = 60/174 (34.48%), Postives = 107/174 (61.49%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+G I HF+ +L  I+ L+K   +RI+  +++  +++R   G+   WC++   + F +  M+G++ E+NEI L+V  + + ++LR     +SVKIKL   R + PCL++ +E  +    +R VTH++PV +VP +IW +Y      ++DV IYLP LK  +  L++MK+ + F+
Sbjct:    9 DVGCINHFIRLLTCISKLTKACTMRITPDHLHFTLSDRASQGAMGLWCELQQANFFDKYQMDGVSAEHNEIYLDVAPESVSRALRTAQAAKSVKIKL--TRKHCPCLTLAVELLTVTGCSRVVTHDIPVYVVPRQIWCDYREPSMPDFDVGIYLPPLKTLKGFLERMKSFSNFV 180
[Close]
BLAST of Checkpoint protein vs. Ensembl Nematostella
Match: EDO49542 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGK3])

HSP 1 Score: 132.109 bits (331), Expect = 3.177e-37
Identity = 68/174 (39.08%), Postives = 107/174 (61.49%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+  +Q F  +L  I+ ++K   LR++ T +Y I  + V +G    WC+++  ++F E  +EG ++ NN I LE++ + L +++R  +  ++VKIKL K  V  PC++ EI   S    TR VTH+VPV ++P R W+EY      + DVSIY+P LKV R ++D+MKNL  FL
Sbjct:    9 DLSCMQRFTRVLGTISRMAKTATLRLTPTKLYFIFADTVASGGISIWCELNQCNIFDEYRIEGTDETNN-IYLELIPENLSRAMRSASNAQAVKIKLTKKHV--PCITFEIILPSLSAHTRTVTHDVPVSVIPQRNWDEYAEPNMPDVDVSIYMPPLKVLRNVVDRMKNLGNFL 179
[Close]
BLAST of Checkpoint protein vs. Ensembl Medaka
Match: zgc:162895 (HUS1 checkpoint clamp component [Source:NCBI gene;Acc:101159622])

HSP 1 Score: 136.732 bits (343), Expect = 2.356e-38
Identity = 65/174 (37.36%), Postives = 107/174 (61.49%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+  + HF  ++  I+ L+K  VLR++   ++ +++ +V  G    WC++   + F E  MEG++ ENNEI LE+  + L ++L+     +SVK+KL K     PCL++  E  +    +R VTH+VPV ++P R+W+E+      +YDVSIYLP LK  + ++D+MKNL+ FL
Sbjct:    9 DVACLNHFTRVVTTISKLTKTCVLRLNPDNLFFVLSGKVANGGVSMWCELSQANFFDEYQMEGVSPENNEICLELAPENLSRALKTIQNAKSVKVKLTKKHC--PCLTVCAELPTLSSISRVVTHDVPVDVIPKRLWHEFKEPSVPDYDVSIYLPPLKTIKNVVDRMKNLSNFL 180
[Close]
BLAST of Checkpoint protein vs. Ensembl Medaka
Match: zgc:162895 (HUS1 checkpoint clamp component [Source:NCBI gene;Acc:101159622])

HSP 1 Score: 135.961 bits (341), Expect = 2.593e-38
Identity = 65/174 (37.36%), Postives = 107/174 (61.49%), Query Frame = 2 
Query:   83 DIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL 604
            D+  + HF  ++  I+ L+K  VLR++   ++ +++ +V  G    WC++   + F E  MEG++ ENNEI LE+  + L ++L+     +SVK+KL K     PCL++  E  +    +R VTH+VPV ++P R+W+E+      +YDVSIYLP LK  + ++D+MKNL+ FL
Sbjct:    9 DVACLNHFTRVVTTISKLTKTCVLRLNPDNLFFVLSGKVANGGVSMWCELSQANFFDEYQMEGVSPENNEICLELAPENLSRALKTIQNAKSVKVKLTKKHC--PCLTVCAELPTLSSISRVVTHDVPVDVIPKRLWHEFKEPSVPDYDVSIYLPPLKTIKNVVDRMKNLSNFL 180
[Close]
BLAST of Checkpoint protein vs. Planmine SMEST
Match: SMESG000026246.1 (SMESG000026246.1)

HSP 1 Score: 301.982 bits (772), Expect = 1.588e-103
Identity = 146/151 (96.69%), Postives = 147/151 (97.35%), Query Frame = 2 
Query:  173 MYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFLRQIFNDS 625
            MYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFL    ND+
Sbjct:    1 MYCIINERVFAGSSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRSVKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIMQPQIEYDVSIYLPNLKVFRTILDKMKNLTGFLIITANDN 151

HSP 2 Score: 116.701 bits (291), Expect = 3.374e-31
Identity = 59/59 (100.00%), Postives = 59/59 (100.00%), Query Frame = 3 
Query:  606 GRSSTTANVKSASVTVDIKRLFQVVNSIKIIPQKLVCNVVDQRCVQIYVLLHDVVIHSI 782
            GRSSTTANVKSASVTVDIKRLFQVVNSIKIIPQKLVCNVVDQRCVQIYVLLHDVVIHSI
Sbjct:  201 GRSSTTANVKSASVTVDIKRLFQVVNSIKIIPQKLVCNVVDQRCVQIYVLLHDVVIHSI 259
The following BLAST results are available for this feature:
BLAST of Checkpoint protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HUS18.535e-3436.00HUS1 checkpoint clamp component [Source:HGNC Symbo... [more]
HUS12.093e-3236.65HUS1 checkpoint clamp component [Source:HGNC Symbo... [more]
HUS11.113e-3136.65HUS1 checkpoint clamp component [Source:HGNC Symbo... [more]
HUS11.113e-3136.65HUS1 checkpoint clamp component [Source:HGNC Symbo... [more]
HUS11.583e-2736.73HUS1 checkpoint clamp component [Source:HGNC Symbo... [more]
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BLAST of Checkpoint protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hus-14.316e-1129.94Checkpoint protein [Source:UniProtKB/TrEMBL;Acc:G... [more]
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BLAST of Checkpoint protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Hus1-like2.713e-1729.51gene:FBgn0026417 transcript:FBtr0334904[more]
Hus1-like2.713e-1729.51gene:FBgn0026417 transcript:FBtr0078876[more]
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BLAST of Checkpoint protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
HUS1B1.529e-3737.14zgc:162895 [Source:NCBI gene;Acc:100037341][more]
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BLAST of Checkpoint protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hus11.089e-3535.06HUS1 checkpoint clamp component [Source:NCBI gene;... [more]
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BLAST of Checkpoint protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Hus11.659e-2734.66HUS1 checkpoint clamp component [Source:MGI Symbol... [more]
Hus11.659e-2734.66HUS1 checkpoint clamp component [Source:MGI Symbol... [more]
Hus1b3.567e-2332.14HUS1 checkpoint clamp component B [Source:MGI Symb... [more]
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BLAST of Checkpoint protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O60921|HUS1_HUMAN4.099e-3336.00Checkpoint protein HUS1 OS=Homo sapiens OX=9606 GN... [more]
sp|Q8BQY8|HUS1_MOUSE2.054e-2834.86Checkpoint protein HUS1 OS=Mus musculus OX=10090 G... [more]
sp|Q8K572|HUS1B_MOUSE2.495e-2232.14Checkpoint protein HUS1B OS=Mus musculus OX=10090 ... [more]
sp|Q8NHY5|HUS1B_HUMAN1.208e-1627.98Checkpoint protein HUS1B OS=Homo sapiens OX=9606 G... [more]
sp|P78955|HUS1_SCHPO1.993e-730.16Checkpoint protein hus1 OS=Schizosaccharomyces pom... [more]
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BLAST of Checkpoint protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3I6X92.749e-4643.65Checkpoint protein OS=Lingula unguis OX=7574 GN=LO... [more]
A0A1S3H1U61.568e-4543.09Checkpoint protein OS=Lingula unguis OX=7574 GN=LO... [more]
A0A087TQI76.406e-4546.47Checkpoint protein HUS1 (Fragment) OS=Stegodyphus ... [more]
R7UB683.114e-4139.56Checkpoint protein OS=Capitella teleta OX=283909 G... [more]
A0A4Y2GWM01.073e-3842.53Checkpoint protein OS=Araneus ventricosus OX=18280... [more]
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BLAST of Checkpoint protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1628951.874e-3736.87HUS1 checkpoint clamp component [Source:NCBI gene;... [more]
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BLAST of Checkpoint protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1628953.015e-3634.48pep scaffold:Pmarinus_7.0:GL478384:20841:26634:-1 ... [more]
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BLAST of Checkpoint protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Checkpoint protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO495423.177e-3739.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Checkpoint protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
zgc:1628952.356e-3837.36HUS1 checkpoint clamp component [Source:NCBI gene;... [more]
zgc:1628952.593e-3837.36HUS1 checkpoint clamp component [Source:NCBI gene;... [more]
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BLAST of Checkpoint protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000026246.11.588e-10396.69SMESG000026246.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021128 ID=SMED30021128|Name=Checkpoint protein|organism=Schmidtea mediterranea sexual|type=transcript|length=782bp
CAAAAAATTTTATTTCTGAATAATTATTTAAATTAATAAAATAAAAAAAA
CTTTTAAAATGAAATTTCGAATGATGAGTAGGGATATTGGAACGATTCAG
CATTTCTTAAATATACTTCAAGGTATTGCGTATTTAAGCAAAAATTTTGT
TTTGCGTATTTCTAATACCTATATGTATTGCATTATAAATGAACGAGTGT
TTGCAGGTTCAAGTCTTTTTTGGTGTCAAATCGATTATAAAAGCCTATTT
TTCGAAAATGCAATGGAAGGCTTAAATCAAGAAAACAACGAAATTCTTTT
AGAAGTTGTATCAGATTATCTAATCAAGAGTCTTCGGCAGTGTGCCACTT
GTCGAAGTGTGAAAATTAAATTAATGAAAAATAGAGTAAATGATCCATGT
CTTTCTATAGAAATTGAACAAAGTAGTGAAATAGACAAGACTCGCTTTGT
AACCCACGAAGTTCCAGTAAGAATTGTTCCACTTCGTATTTGGAATGAAT
ATATTATGCAACCTCAAATAGAATATGATGTCAGCATATATTTACCCAAT
TTAAAAGTTTTCCGAACCATCTTAGATAAAATGAAAAATCTCACTGGATT
TCTGAGGCAGATCTTCAACGACAGCAAACGTTAAAAGCGCATCGGTAACC
GTTGACATAAAGCGATTGTTTCAAGTTGTAAATTCTATTAAAATTATTCC
TCAAAAATTAGTCTGCAATGTTGTTGATCAAAGATGTGTTCAGATATATG
TTTTGTTGCACGATGTAGTGATCCATAGCATT
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protein sequence of SMED30021128-orf-1

>SMED30021128-orf-1 ID=SMED30021128-orf-1|Name=SMED30021128-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=192bp
MKFRMMSRDIGTIQHFLNILQGIAYLSKNFVLRISNTYMYCIINERVFAG
SSLFWCQIDYKSLFFENAMEGLNQENNEILLEVVSDYLIKSLRQCATCRS
VKIKLMKNRVNDPCLSIEIEQSSEIDKTRFVTHEVPVRIVPLRIWNEYIM
QPQIEYDVSIYLPNLKVFRTILDKMKNLTGFLRQIFNDSKR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000075cell cycle checkpoint
GO:0000077DNA damage checkpoint
GO:0006281DNA repair
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR007150Hus1/Mec3
IPR016580Cell_cycle_HUS1
Vocabulary: cellular component
TermDefinition
GO:0030896checkpoint clamp complex
GO:0005730nucleolus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.70.10.10coord: 1..190
e-value: 5.4E-51
score: 175.1
NoneNo IPR availablePANTHERPTHR12900:SF4CHECKPOINT PROTEIN HUS1coord: 1..181
NoneNo IPR availablePANTHERPTHR12900MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1coord: 1..181
IPR016580Cell cycle checkpoint, Hus1PIRSFPIRSF011312HUS1coord: 1..188
e-value: 4.5E-41
score: 138.2
IPR007150Checkpoint protein Hus1/Mec3PFAMPF04005Hus1coord: 1..178
e-value: 3.4E-35
score: 121.6