Tyrosine--tRNA ligase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021122 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of Tyrosine--tRNA ligase vs. Ensembl Human
Match: YARS1 (tyrosyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:12840]) HSP 1 Score: 364.77 bits (935), Expect = 4.273e-117 Identity = 186/363 (51.24%), Postives = 252/363 (69.42%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKS 1139
+ ++++TRNLQE +G EKL + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R YYE +IK ML+++ VPL+KL+F++GT+YQL++ YT +VY++ + + HD+ KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGG+DQRKIF +AEK L LG++KR HLMNPM+PGLTG KMS+SEE SKIDLLD + V KL A C + NG+L+FIK V+ P+KS+ I K Y ++E DF +++ LK+ VE AL L+ ++E+F + L AYP D + +A + QN +S
Sbjct: 8 EEKLHLITRNLQEVLGEEKLKEIL-KERELKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYP------DPSKQKPMAKGLPRIQNQRRS 363 HSP 2 Score: 125.176 bits (313), Expect = 4.407e-30 Identity = 95/319 (29.78%), Postives = 168/319 (52.66%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIK 2153
KI W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R Y+ I + L + L ++FI G+DYQLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + ID+ + FA K L + K V++ + + L G + M S + ++K I + D + + +KLKKAF +P +++ + L+ ++H+++P F++ R + GG+ Y++ + D +++HPGD+K+++ LN + + PI+
Sbjct: 37 KIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGIDQRKIFTFAEKYLPALGYSKR--VHLMNPMVPGLTG-SKMSSSEEESK--------IDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPL---KSEFVILRDEKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALN-KLLDPIR 325
BLAST of Tyrosine--tRNA ligase vs. Ensembl Human
Match: YARS1 (tyrosyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:12840]) HSP 1 Score: 364.77 bits (935), Expect = 3.490e-115 Identity = 181/336 (53.87%), Postives = 243/336 (72.32%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
+ ++++TRNLQE +G EKL + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R YYE +IK ML+++ VPL+KL+F++GT+YQL++ YT +VY++ + + HD+ KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGG+DQRKIF +AEK L LG++KR HLMNPM+PGLTG KMS+SEE SKIDLLD + V KL A C + NG+L+FIK V+ P+KS+ I K Y ++E DF +++ LK+ VE AL L+ ++E+F + L AYP
Sbjct: 8 EEKLHLITRNLQEVLGEEKLKEIL-KERELKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYP 342 HSP 2 Score: 137.117 bits (344), Expect = 3.590e-33 Identity = 110/373 (29.49%), Postives = 192/373 (51.47%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSX-XXXXXXXXXXXAFPSERKHKFKHDKNAFN-------PNSLNIRVARIVSIE 2291
KI W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R Y+ I + L + L ++FI G+DYQLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + ID+ + FA K L + K V++ + + L G + M S + ++K I + D + + +KLKKAF +P +++ + L+ ++H+++P F++ R + GG+ Y++ + D +++HPGD+K+++ LN + + PI+ L++L A+P K K A N P+ L+IRV +I+++E
Sbjct: 37 KIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGIDQRKIFTFAEKYLPALGYSKR--VHLMNPMVPGLTG-SKMSSSEEESK--------IDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPL---KSEFVILRDEKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALN-KLLDPIREKFNTPALKKLASAAYPDPSKQKPMAKGPAKNSEPEEVIPSRLDIRVGKIITVE 379
BLAST of Tyrosine--tRNA ligase vs. Ensembl Celegans
Match: yars-1 (Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Acc:G5ED95]) HSP 1 Score: 366.311 bits (939), Expect = 7.490e-114 Identity = 253/710 (35.63%), Postives = 391/710 (55.07%), Query Frame = 3
Query: 84 IVTRNLQECIGTEKLDQCIDK-NKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLL-KKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQN-PPTELAN--TISGYQNNLKSLL---------EEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWK---ITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQAD-LSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDL-------LHPGDVKSALVEYLNERF 2138
++ RNLQE +G +KL + ++ K ++YWGTA TG+PH+ Y VPM KIADFL+AG VTILFADLHAYLDNMK+ WELL+ R YYE +IK +L++L VP+++L F +GTEYQL+ YT +V ++ S DA KAG +VVKQ+ PL+SG++YPLLQALDE++L D QFGGVDQRKIF+ AE+ L L KR HLMNPM+PGLTG KMS+SEE+SKID+LD + V K+ A C+ NG+L+F FV+ P+ S I++ +++ I+ ++ + + LDEN+LK+ + L +L+ ++ + ++ Y T+ + PP+E ++S Q K L EE + L+ D P P +++ G H+G++ PL K+ +S TIL+ + A L ++ K AW I+ R +++ S + + + D L +E D L REY L Y+ S V+ RD S + + + + GNL L+Y L+ + +P + ++ S + F D ++L +P + I TI NG M D P DT + K+ ++F +P++LD + ++ + EH+++P + ++R +NGGDV+ ++ Q+ + V LHP D+K+A+V +N F
Sbjct: 22 LILRNLQESLGVDKLTKQLETPGKVPHVYWGTATTGKPHVGYLVPMRKIADFLQAGLKVTILFADLHAYLDNMKSSWELLKCRVIYYENVIKALLQSLDVPIEQLYFKKGTEYQLSREYTDDVLRLSAQVSQRDALKAGAEVVKQVESPLLSGLLYPLLQALDEQYLKVDGQFGGVDQRKIFILAEEQLPKLKLGKRWHLMNPMVPGLTGTKMSSSEEDSKIDVLDEPEKVRSKIMGAACSR-DQPDNGVLSFYNFVLFPIVSPNAIEIENQQFFDIDSLKRAYLDGKLDENTLKTFLADFLVNLLEKVRVRCDTDVVRDAKEKGYNHSTDSTEPAIPPSEAPKIPSLSSEQKQWKDQLLNGNEILIGEELDRVLA---DVSPQKP--LHLMFIAPGKGRFHLGFIAPLLKIKEISDAGGVPVIATILVSPLEAFLDNE----KVAWAAIQSRAVYFREMFISLIKHLKLDGLVKVEIAGEHDKYLDREYVLDFYKMASAVS-RDESTVC--------DGNSLSGNLVPLIY----ALNAQMTRPDILIIGADSQV--FADLCSRLLRSFGYPAIAHLLI--PTIPGCNG-QKMSCSIPDFLLDPLDTPK--------QLKTKIARSFCEPQNLDGNVAMQLAEHIVFPIL-NGAALHIQRAPDNGGDVEVTNYKQLEQEFVIGTKNEQQFPLHPADLKNAVVGVINRLF 694
BLAST of Tyrosine--tRNA ligase vs. Ensembl Fly
Match: TyrRS (gene:FBgn0027080 transcript:FBtr0075410) HSP 1 Score: 350.903 bits (899), Expect = 3.176e-110 Identity = 171/334 (51.20%), Postives = 236/334 (70.66%), Query Frame = 3
Query: 84 IVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPV-KSQLNIDLLKK--------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++TRNLQE +G +KL + + ++ +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW LL+ RT+YYE +IK ML ++ VPL+KL+FV+G++YQL++ YT +VYK+ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF ++EK L LG+ KR H MNPM+PGL GGKMS+SEE+SKIDLLD+ +V KL A C + + NG+L+F+K V+ + K ++ ++ + E++E + L LK+ VE + L+ +++ F + L AYP
Sbjct: 12 LITRNLQETLGDDKLTKIL-AERDLKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWSLLELRTKYYEQVIKAMLSSIGVPLEKLKFVKGSDYQLSKEYTLDVYKLSSVVTQHDAKKAGAEVVKQVEYPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFSEKYLPQLGYEKRIHFMNPMVPGLAGGKMSSSEEDSKIDLLDSPANVKKKLKKAFCEPGNIADNGLLSFVKHVLFSLFKEGEGFEVNREAEHGGDVTFLKYEDLEKYYAEDKLHPGDLKATVEKYINRLLDPIRKAFENPELQKLSAAAYP 344 HSP 2 Score: 156.762 bits (395), Expect = 4.021e-40 Identity = 111/369 (30.08%), Postives = 194/369 (52.57%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFP----SERKHKFKHDKNAFNPNSLNIRVARIVSI 2288
KI W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L++ L ++F+ GSDYQLS+EY L +Y+ S VT+ D + +++ Q +P LS L+YP L LD+ LK + A + +D+ + F+ K +L + K ++ + + L G M S + D+K I + DS + +KLKKAF +P ++ + L+ ++H+++ F++ E F V R E+GGDV + + + D LHPGD+K+ + +Y+N R + PI K+ +L++L A+P + + P+ L+IRV ++V +
Sbjct: 36 KIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWSLLELRTKYYEQVIKAMLSSIGVPLEKLKFVKGSDYQLSKEYTLDVYKLSSVVTQHDAKKAGAEVVKQVEYPL-------LSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFSEKYLPQLGYEKR--IHFMNPMVPGLAG-GKMSSSEEDSK--------IDLLDSPANVKKKLKKAFCEPGNIADNGLLSFVKHVLFSLFKEGEGFEVNREAEHGGDVTFLKYEDLEKYYAEDKLHPGDLKATVEKYIN-RLLDPIRKAFENPELQKLSAAAYPPPAKVKAGAAPAAGADEDAPHRLDIRVGKVVEV 377
BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:368235]) HSP 1 Score: 368.622 bits (945), Expect = 7.039e-117 Identity = 183/336 (54.46%), Postives = 239/336 (71.13%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKK------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++ ++TRNLQE +G E+L + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPLDKL+FV+GT+YQL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KR+HLMNPM+PGLTG KMS+SEE SKIDLLD V KL A C + NG+L+F+K V+ P+ S+ I K Y EE+E DF + + LK+ VE AL L+ ++++F + L AYP
Sbjct: 8 DEKFQLITRNLQEVLGEERLKEIL-KERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYTKRSHLMNPMVPGLTGSKMSSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSEFVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALNKLLDPIRKKFESPELKKLTSSAYP 342 HSP 2 Score: 155.992 bits (393), Expect = 1.106e-39 Identity = 109/372 (29.30%), Postives = 191/372 (51.34%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSERKHKF--------KHDKNAFNPNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L ++F+ G+DYQLSREY L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P H + GLT K ++ + + I + D + +KLKKAF +P +++ + L+ ++H+++P + F++KR + GGD Y+ +++ D + +HPGD+K+++ LN + + PI K +L++L A+P K+K D + P+ L+IRV +++S+E
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYTKRSHLMNPMVPGLTGSKM------SSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSE---FVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALN-KLLDPIRKKFESPELKKLTSSAYPEPSKNKGGAKGNPKQTTDDDEVIPSRLDIRVGKVISVE 380
BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:368235]) HSP 1 Score: 368.622 bits (945), Expect = 7.039e-117 Identity = 183/336 (54.46%), Postives = 239/336 (71.13%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKK------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++ ++TRNLQE +G E+L + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPLDKL+FV+GT+YQL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KR+HLMNPM+PGLTG KMS+SEE SKIDLLD V KL A C + NG+L+F+K V+ P+ S+ I K Y EE+E DF + + LK+ VE AL L+ ++++F + L AYP
Sbjct: 8 DEKFQLITRNLQEVLGEERLKEIL-KERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYTKRSHLMNPMVPGLTGSKMSSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSEFVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALNKLLDPIRKKFESPELKKLTSSAYP 342 HSP 2 Score: 155.992 bits (393), Expect = 1.106e-39 Identity = 109/372 (29.30%), Postives = 191/372 (51.34%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSERKHKF--------KHDKNAFNPNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L ++F+ G+DYQLSREY L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P H + GLT K ++ + + I + D + +KLKKAF +P +++ + L+ ++H+++P + F++KR + GGD Y+ +++ D + +HPGD+K+++ LN + + PI K +L++L A+P K+K D + P+ L+IRV +++S+E
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYTKRSHLMNPMVPGLTGSKM------SSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSE---FVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALN-KLLDPIRKKFESPELKKLTSSAYPEPSKNKGGAKGNPKQTTDDDEVIPSRLDIRVGKVISVE 380
BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:368235]) HSP 1 Score: 368.622 bits (945), Expect = 7.039e-117 Identity = 183/336 (54.46%), Postives = 239/336 (71.13%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKK------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++ ++TRNLQE +G E+L + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPLDKL+FV+GT+YQL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KR+HLMNPM+PGLTG KMS+SEE SKIDLLD V KL A C + NG+L+F+K V+ P+ S+ I K Y EE+E DF + + LK+ VE AL L+ ++++F + L AYP
Sbjct: 8 DEKFQLITRNLQEVLGEERLKEIL-KERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYTKRSHLMNPMVPGLTGSKMSSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSEFVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALNKLLDPIRKKFESPELKKLTSSAYP 342 HSP 2 Score: 155.992 bits (393), Expect = 1.106e-39 Identity = 109/372 (29.30%), Postives = 191/372 (51.34%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSERKHKF--------KHDKNAFNPNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L ++F+ G+DYQLSREY L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P H + GLT K ++ + + I + D + +KLKKAF +P +++ + L+ ++H+++P + F++KR + GGD Y+ +++ D + +HPGD+K+++ LN + + PI K +L++L A+P K+K D + P+ L+IRV +++S+E
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYTKRSHLMNPMVPGLTGSKM------SSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSE---FVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALN-KLLDPIRKKFESPELKKLTSSAYPEPSKNKGGAKGNPKQTTDDDEVIPSRLDIRVGKVISVE 380
BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:368235]) HSP 1 Score: 347.436 bits (890), Expect = 3.623e-112 Identity = 164/271 (60.52%), Postives = 210/271 (77.49%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI 881
++ ++TRNLQE +G E+L + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPLDKL+FV+GT+YQL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KR+HLMNPM+PGLTG KMS+SEE SKIDLLD V KL A C + NG+L+F+K V+ P+ S+ I
Sbjct: 8 DEKFQLITRNLQEVLGEERLKEIL-KERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYTKRSHLMNPMVPGLTGSKMSSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSEFVI 277 HSP 2 Score: 122.094 bits (305), Expect = 3.735e-30 Identity = 83/277 (29.96%), Postives = 141/277 (50.90%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGD 2033
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L ++F+ G+DYQLSREY L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P H + GLT K ++ + + I + D + +KLKKAF +P +++ + L+ ++H+++P + F++KR + GGD
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYTKRSHLMNPMVPGLTGSKM------SSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSE---FVIKRDPKFGGD 286
BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:368235]) HSP 1 Score: 325.094 bits (832), Expect = 3.042e-104 Identity = 156/244 (63.93%), Postives = 194/244 (79.51%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANC 800
++ ++TRNLQE +G E+L + I K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPLDKL+FV+GT+YQL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KR+HLMNPM+PGLTG KMS+SEE SKIDLLD V KL A C
Sbjct: 8 DEKFQLITRNLQEVLGEERLKE-ILKERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYTKRSHLMNPMVPGLTGSKMSSSEEESKIDLLDKNQDVKKKLKKAFC 250 HSP 2 Score: 101.293 bits (251), Expect = 2.432e-23 Identity = 73/237 (30.80%), Postives = 116/237 (48.95%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAF 1913
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L ++F+ G+DYQLSREY L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P H + GLT K ++ + + I + D + +KLKKAF
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYTKRSHLMNPMVPGLTGSK------MSSSEEESKIDLLDKNQDVKKKLKKAF 249
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:448444]) HSP 1 Score: 369.007 bits (946), Expect = 8.253e-118 Identity = 176/324 (54.32%), Postives = 234/324 (72.22%), Query Frame = 3
Query: 105 ECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQL------NIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
E +G +K+ + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW+LL+ RT YYE +I+ ML+++ VPL++LRF+RGTE+QL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE SKIDLLD+ V KL A C + NG+L+F++ V+ P+KS+ K Y E +E DF +L+ LK+ VE AL L+ ++E+F + L +AYP
Sbjct: 4 EVLGEDKMKEIL-KERPLRIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAKMSSSEEESKIDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPLKSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALNKLLDPIREKFNSPEMKKLSNDAYP 326 HSP 2 Score: 140.969 bits (354), Expect = 5.314e-35 Identity = 94/319 (29.47%), Postives = 168/319 (52.66%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIK 2153
+I W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L + L + FI G+++QLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + ++K I + DS + +KLKKAF +P +++ + L+ + H+++P F+V R + GG+ Y+ + + D L+HPGD+K+++ + LN + + PI+
Sbjct: 21 RIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYAKR--IHLMNPMVPGLTG-AKMSSSEEESK--------IDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPL---KSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALN-KLLDPIR 309
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:448444]) HSP 1 Score: 366.311 bits (939), Expect = 5.015e-117 Identity = 174/313 (55.59%), Postives = 228/313 (72.84%), Query Frame = 3
Query: 138 IDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
I K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW+LL+ RT YYE +I+ ML+++ VPL++LRF+RGTE+QL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE SKIDLLD+ V KL A C + NG+L+F++ V+ P+KS+ + K Y E +E DF +L+ LK+ VE AL L+ ++E+F + L +AYP
Sbjct: 4 ILKERPLRIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAKMSSSEEESKIDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPLKSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALNKLLDPIREKFNSPEMKKLSNDAYP 316 HSP 2 Score: 141.354 bits (355), Expect = 2.931e-35 Identity = 94/319 (29.47%), Postives = 168/319 (52.66%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIK 2153
+I W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L + L + FI G+++QLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + ++K I + DS + +KLKKAF +P +++ + L+ + H+++P F+V R + GG+ Y+ + + D L+HPGD+K+++ + LN + + PI+
Sbjct: 11 RIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYAKR--IHLMNPMVPGLTG-AKMSSSEEESK--------IDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPL---KSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALN-KLLDPIR 299
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:448444]) HSP 1 Score: 365.925 bits (938), Expect = 1.173e-116 Identity = 174/313 (55.59%), Postives = 227/313 (72.52%), Query Frame = 3
Query: 138 IDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQL------NIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
I K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW+LL+ RT YYE +I+ ML+++ VPL++LRF+RGTE+QL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE SKIDLLD+ V KL A C + NG+L+F++ V+ P+KS+ K Y E +E DF +L+ LK+ VE AL L+ ++E+F + L +AYP
Sbjct: 4 ILKERPLRIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAKMSSSEEESKIDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPLKSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALNKLLDPIREKFNSPEMKKLSNDAYP 316 HSP 2 Score: 140.969 bits (354), Expect = 5.489e-35 Identity = 94/319 (29.47%), Postives = 168/319 (52.66%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIK 2153
+I W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L + L + FI G+++QLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + ++K I + DS + +KLKKAF +P +++ + L+ + H+++P F+V R + GG+ Y+ + + D L+HPGD+K+++ + LN + + PI+
Sbjct: 11 RIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYAKR--IHLMNPMVPGLTG-AKMSSSEEESK--------IDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPL---KSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALN-KLLDPIR 299
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:448444]) HSP 1 Score: 365.54 bits (937), Expect = 3.200e-116 Identity = 174/313 (55.59%), Postives = 227/313 (72.52%), Query Frame = 3
Query: 138 IDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQL------NIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
I K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW+LL+ RT YYE +I+ ML+++ VPL++LRF+RGTE+QL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE SKIDLLD+ V KL A C + NG+L+F++ V+ P+KS+ K Y E +E DF +L+ LK+ VE AL L+ ++E+F + L +AYP
Sbjct: 4 ILKERPLRIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAKMSSSEEESKIDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPLKSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALNKLLDPIREKFNSPEMKKLSNDAYP 316 HSP 2 Score: 140.969 bits (354), Expect = 7.748e-35 Identity = 94/319 (29.47%), Postives = 168/319 (52.66%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIK 2153
+I W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L + L + FI G+++QLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + ++K I + DS + +KLKKAF +P +++ + L+ + H+++P F+V R + GG+ Y+ + + D L+HPGD+K+++ + LN + + PI+
Sbjct: 11 RIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYAKR--IHLMNPMVPGLTG-AKMSSSEEESK--------IDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPL---KSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALN-KLLDPIR 299
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:448444]) HSP 1 Score: 366.311 bits (939), Expect = 3.210e-116 Identity = 174/313 (55.59%), Postives = 228/313 (72.84%), Query Frame = 3
Query: 138 IDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
I K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW+LL+ RT YYE +I+ ML+++ VPL++LRF+RGTE+QL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE SKIDLLD+ V KL A C + NG+L+F++ V+ P+KS+ + K Y E +E DF +L+ LK+ VE AL L+ ++E+F + L +AYP
Sbjct: 4 ILKERPLRIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAKMSSSEEESKIDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPLKSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALNKLLDPIREKFNSPEMKKLSNDAYP 316 HSP 2 Score: 140.198 bits (352), Expect = 1.552e-34 Identity = 94/319 (29.47%), Postives = 168/319 (52.66%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIK 2153
+I W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L + L + FI G+++QLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + ++K I + DS + +KLKKAF +P +++ + L+ + H+++P F+V R + GG+ Y+ + + D L+HPGD+K+++ + LN + + PI+
Sbjct: 11 RIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYAKR--IHLMNPMVPGLTG-AKMSSSEEESK--------IDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPL---KSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALN-KLLDPIR 299
BLAST of Tyrosine--tRNA ligase vs. Ensembl Mouse
Match: Yars (tyrosyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:2147627]) HSP 1 Score: 371.318 bits (952), Expect = 2.584e-117 Identity = 188/349 (53.87%), Postives = 250/349 (71.63%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTE 1097
+ ++++TRNLQE +G EKL + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ RT YYE +IK ML+++ VPL+KL+F++GT+YQL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG++KR HLMNPM+PGLTG KMS+SEE SKIDLLD + V KL A C + NG+L+FIK V+ P+KS+ I K Y E+E DF +++ LK+ VE AL L+ ++E+F + L AYP ++ Q PP +
Sbjct: 44 EEKLHLITRNLQEVLGEEKLKEIL-KERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRTSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTVYLELEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSK---QKPPAK 388 HSP 2 Score: 136.732 bits (343), Expect = 4.304e-33 Identity = 109/373 (29.22%), Postives = 192/373 (51.47%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSX-XXXXXXXXXXXAFPSERKHKFKHDKNAFN-------PNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R Y+ I + L + L ++FI G+DYQLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K V++ + + L G + M S + ++K I + D + + +KLKKAF +P +++ + L+ ++H+++P F++ R + GG+ Y+ ++ D +++HPGD+K+++ LN + + PI+ L++L A+P K K A N P+ L+IRV +I+S+E
Sbjct: 73 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRTSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYSKR--VHLMNPMVPGLTG-SKMSSSEEESK--------IDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPL---KSEFVILRDEKWGGNKTYTVYLELEKDFAAEVVHPGDLKNSVEVALN-KLLDPIREKFNTPALKKLASAAYPDPSKQKPPAKGPAKNSEPEEVIPSRLDIRVGKILSVE 415
BLAST of Tyrosine--tRNA ligase vs. Ensembl Mouse
Match: Yars (tyrosyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:2147627]) HSP 1 Score: 96.6709 bits (239), Expect = 4.815e-23 Identity = 49/86 (56.98%), Postives = 63/86 (73.26%), Query Frame = 3
Query: 258 TILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQ 515
TILFADLHAYLDNMKAPWELL+ RT YYE +IK ML+++ VPL+KL+F++GT+YQL++ A+V K H A + C KQ
Sbjct: 2 TILFADLHAYLDNMKAPWELLELRTSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSK-LCAHVCKY-----RHTAYRHKCQAGKQ 81
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q6DIJ1|SYYC_XENTR (Tyrosine--tRNA ligase, cytoplasmic OS=Xenopus tropicalis OX=8364 GN=yars1 PE=2 SV=1) HSP 1 Score: 382.874 bits (982), Expect = 2.214e-121 Identity = 181/332 (54.52%), Postives = 242/332 (72.89%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++TRNLQE +G +K+ + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW+LL+ RT YYE +I+ ML+++ VPL++LRF+RGTE+QL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE SKIDLLD+ V KL A C + NG+L+F++ V+ P+KS+ + K Y E +E DF +L+ LK+ VE AL L+ ++E+F + L +AYP
Sbjct: 12 QLITRNLQEVLGEDKMKEIL-KERPLRIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAKMSSSEEESKIDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPLKSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALNKLLDPIREKFNSPEMKKLSNDAYP 342 HSP 2 Score: 148.673 bits (374), Expect = 2.547e-36 Identity = 115/410 (28.05%), Postives = 206/410 (50.24%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSX-XXXXXXXXXXXAFPSERKHKF---KHDKNA----FNPNSLNIRVARIVSIEY---VDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCL 2393
+I W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L + L + FI G+++QLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + ++K I + DS + +KLKKAF +P +++ + L+ + H+++P F+V R + GG+ Y+ + + D L+HPGD+K+++ + LN + + PI+ ++++L A+P K K KN+ +P+ L++RV +I+S+ D Y + G E S + + P+D L
Sbjct: 37 RIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYAKR--IHLMNPMVPGLTG-AKMSSSEEESK--------IDLLDSPADVKKKLKKAFCEPGNIENNGVLSFVRHVLFPL---KSEFVVLRDEKFGGNKTYTDFETLEKDFTEQLVHPGDLKASVEKALN-KLLDPIREKFNSPEMKKLSNDAYPGASKQKTVPKGSTKNSGPEEIDPSLLDLRVGKILSVRQHPDADSLYVESVDVGEENPRCVVSGLVQYVPSDQLL 416
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q7ZX51|SYYC_XENLA (Tyrosine--tRNA ligase, cytoplasmic OS=Xenopus laevis OX=8355 GN=yars1 PE=2 SV=1) HSP 1 Score: 381.719 bits (979), Expect = 5.802e-121 Identity = 181/332 (54.52%), Postives = 242/332 (72.89%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++TRNLQE +G +K+ + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPW+LL+ RT YYE +I+ ML+++ VPL++LRF+RGTE+QL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE SKIDLLD+ V KL A C + NG+L+F++ V+ P+KS+ + K Y E +E DF +L+ LK+ VE AL L+ ++E+F + L +AYP
Sbjct: 12 QLITRNLQELLGEDKMKEIL-KERPLRIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAKMSSSEEESKIDLLDSPADVKKKLKKAFCEPGNVENNGVLSFVRHVLFPLKSEFVVLRDEKFGGNKTYTDFETLEKDFAEELVHPGDLKASVEKALNKLLHPIREKFNSPEMKKLSNDAYP 342 HSP 2 Score: 148.673 bits (374), Expect = 2.618e-36 Identity = 107/372 (28.76%), Postives = 195/372 (52.42%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSX-XXXXXXXXXXXAFPSERKHKF---KHDKNA----FNPNSLNIRVARIVSI 2288
+I W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W+L+ R + Y+Q I + L + L + FI G+++QLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + ++K I + DS + +KLKKAF +P +++ + L+ + H+++P F+V R + GG+ Y+ + + D +L+HPGD+K+++ + LN + + PI+ ++++L A+P K K KN+ +P+ L++RV +I+S+
Sbjct: 37 RIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWDLLELRTRYYEQVIQAMLQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYAKR--IHLMNPMVPGLTG-AKMSSSEEESK--------IDLLDSPADVKKKLKKAFCEPGNVENNGVLSFVRHVLFPL---KSEFVVLRDEKFGGNKTYTDFETLEKDFAEELVHPGDLKASVEKALN-KLLHPIREKFNSPEMKKLSNDAYPDASKQKSVPKGSTKNSGTEEIDPSLLDLRVGKILSV 378
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q5ZJ08|SYYC_CHICK (Tyrosine--tRNA ligase, cytoplasmic OS=Gallus gallus OX=9031 GN=YARS1 PE=2 SV=1) HSP 1 Score: 380.948 bits (977), Expect = 1.231e-120 Identity = 187/332 (56.33%), Postives = 242/332 (72.89%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKK------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++TRNLQE +G +KL I K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ RT YYE +IK ML+++ VPL+KL+F+RGT+YQL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG+AKR HLMNPM+PGLTG KMS+SEE+SKIDLLD + V KL A C + NG+L+FIK V+ P+KS+ I +K Y E +E DF +++ LK+ VE AL L+ ++E+F + L AYP
Sbjct: 12 QLITRNLQEVLGEDKL-MAILKEREVKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRTRYYEHVIKAMLESIGVPLEKLKFIRGTDYQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPSLGYAKRIHLMNPMVPGLTGSKMSSSEEDSKIDLLDRKEDVKKKLKKAFCEPGNIENNGVLSFIKHVLFPLKSEFVILREEKWGGNKTYTAYETLEKDFAEQVVHPGDLKNSVEAALNKLLDPIREKFNSPELKKLTNAAYP 342 HSP 2 Score: 146.362 bits (368), Expect = 1.502e-35 Identity = 110/372 (29.57%), Postives = 193/372 (51.88%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSXXXXXXXXXXXXAF---PSERKHKFKHDKNA----FNPNSLNIRVARIVSIE 2291
KI W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+ I + L + L ++FI G+DYQLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G + M S + D+K I + D + + +KLKKAF +P +++ + L+ ++H+++P F++ R + GG+ Y++ + + D ++HPGD+K+++ LN + + PI+ + L A PS+ K K KN+ P+ L+IRV ++VS+E
Sbjct: 37 KIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRTRYYEHVIKAMLESIGVPLEKLKFIRGTDYQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPSLGYAKR--IHLMNPMVPGLTG-SKMSSSEEDSK--------IDLLDRKEDVKKKLKKAFCEPGNIENNGVLSFIKHVLFPL---KSEFVILREEKWGGNKTYTAYETLEKDFAEQVVHPGDLKNSVEAALN-KLLDPIREKFNSPELKKLTNAAYPNPSKAKPAEKGTKNSEPETIVPSRLDIRVGKVVSVE 378
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q91WQ3|SYYC_MOUSE (Tyrosine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Yars1 PE=1 SV=3) HSP 1 Score: 371.318 bits (952), Expect = 6.647e-117 Identity = 188/349 (53.87%), Postives = 250/349 (71.63%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNI------DLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTE 1097
+ ++++TRNLQE +G EKL + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ RT YYE +IK ML+++ VPL+KL+F++GT+YQL++ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L LG++KR HLMNPM+PGLTG KMS+SEE SKIDLLD + V KL A C + NG+L+FIK V+ P+KS+ I K Y E+E DF +++ LK+ VE AL L+ ++E+F + L AYP ++ Q PP +
Sbjct: 8 EEKLHLITRNLQEVLGEEKLKEIL-KERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRTSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTVYLELEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSK---QKPPAK 352 HSP 2 Score: 136.732 bits (343), Expect = 2.353e-32 Identity = 109/373 (29.22%), Postives = 192/373 (51.47%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSX-XXXXXXXXXXXAFPSERKHKFKHDKNAFN-------PNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R Y+ I + L + L ++FI G+DYQLS+EY L +Y+ S VT+ D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + FA K L + K V++ + + L G + M S + ++K I + D + + +KLKKAF +P +++ + L+ ++H+++P F++ R + GG+ Y+ ++ D +++HPGD+K+++ LN + + PI+ L++L A+P K K A N P+ L+IRV +I+S+E
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRTSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPALGYSKR--VHLMNPMVPGLTG-SKMSSSEEESK--------IDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPL---KSEFVILRDEKWGGNKTYTVYLELEKDFAAEVVHPGDLKNSVEVALN-KLLDPIREKFNTPALKKLASAAYPDPSKQKPPAKGPAKNSEPEEVIPSRLDIRVGKILSVE 379
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q6TGS6|SYYC_DANRE (Tyrosine--tRNA ligase, cytoplasmic OS=Danio rerio OX=7955 GN=yars1 PE=2 SV=2) HSP 1 Score: 368.622 bits (945), Expect = 5.484e-116 Identity = 183/336 (54.46%), Postives = 239/336 (71.13%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKK------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++ ++TRNLQE +G E+L + + K + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPLDKL+FV+GT+YQL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KR+HLMNPM+PGLTG KMS+SEE SKIDLLD V KL A C + NG+L+F+K V+ P+ S+ I K Y EE+E DF + + LK+ VE AL L+ ++++F + L AYP
Sbjct: 8 DEKFQLITRNLQEVLGEERLKEIL-KERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYTKRSHLMNPMVPGLTGSKMSSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSEFVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALNKLLDPIRKKFESPELKKLTSSAYP 342 HSP 2 Score: 155.992 bits (393), Expect = 8.272e-39 Identity = 109/372 (29.30%), Postives = 191/372 (51.34%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSERKHKF--------KHDKNAFNPNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L ++F+ G+DYQLSREY L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P H + GLT K ++ + + I + D + +KLKKAF +P +++ + L+ ++H+++P + F++KR + GGD Y+ +++ D + +HPGD+K+++ LN + + PI K +L++L A+P K+K D + P+ L+IRV +++S+E
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYTKRSHLMNPMVPGLTGSKM------SSSEEESKIDLLDKNQDVKKKLKKAFCEPGNVENNGVLSFVKHVLFPLHSE---FVIKRDPKFGGDKVYTDFEEVEKDFAAEQIHPGDLKASVELALN-KLLDPIRKKFESPELKKLTSSAYPEPSKNKGGVKGNPKQTTDDDEVIPSRLDIRVGKVISVE 380
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A1I8ILR9 (Tyrosine--tRNA ligase OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 491.886 bits (1265), Expect = 4.713e-156 Identity = 304/764 (39.79%), Postives = 458/764 (59.95%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPV---KSQLNIDLLKK-YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGK-ENLDQEAYPIE--TEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSI------SLDTFPDIPG-SPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGS---DYQLSREYALLLYQSFSHVTERDCSEMLLQMI--GQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVA-DYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWP----SFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVK----SALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSERKHKFKHDKNAFNPNSLNIRVARIVSI 2288
++ RNLQE +G ++L K++ ++YWGTA TGRPH+AYFVPM+K+AD+L AGC VT+LFADLHAYLDNMKAPW LL KRT YYE +IKGMLK++ VPLD+L+FVRGT+++L + YT +VY+I + HDA KAG +VVKQ++ PLISG++YP LQALDE++L DAQFGGVDQRKIFM AEK L +LG++KR HLMNPMIPGLTGGKMSASE +SKIDLLD+A+SVS K++ A C+ ++ NG+L+F+++V+ P+ L I KK Y +++ DF + L LK V +L L+G +++EF +S + + L +AYP + + ++A+ ++G Q L ++ + ++LS S D+ P G SP++LW++R +G H+G+L+PL +++ WK+T+L+ ++ A L + + + RG K +S T D + S + +++Y L +Y+ S VT + + ++ G+HP LS L++PCLS LD + +VRL D S + F A RL +P++ P Y H + L G ++M D ++I +SA ++ +K+KK F +P ++DR P L+ L L+ P S + + V R+ E GGD+ ++S D+++ V LHPGD+K S L+E N ++ L+G +L + L AF + K + NP+ L+IRV RI+ +
Sbjct: 6 QLIIRNLQEVVGLDRLKAKTAKHE-LSVYWGTATTGRPHVAYFVPMSKLADYLLAGCHVTVLFADLHAYLDNMKAPWSLLNKRTNYYETVIKGMLKSIGVPLDRLKFVRGTDFELGKEYTRDVYRITAEVTVHDAKKAGAEVVKQVASPLISGLLYPCLQALDEQYLGVDAQFGGVDQRKIFMLAEKYLVNLGYSKRIHLMNPMIPGLTGGKMSASEADSKIDLLDSAESVSAKVAGAQCSPGAAEGNGVLSFLQYVVFPLLGGTDGLAIGSSKKRYAEFQQVSEDFASGSLTAEDLKGCVADSLNRLLGPVRKEFEESSELQQLLVDAYPKDQPAKGSSGGDAGDVADRLAGIQ--LATIDAKLIESLSRFATQFGSQDSVPVKSGSSPRLLWTIRPSGPMHLGHLVPLIGALSMAKAGWKVTLLVSEMQAFL----DRKRAPIDSLEVRGSTVKDVLSRLATGLLGDTAAAAVTVTSGLEELSYNKDYVLDMYRLVSLVTVDEAMQAGSGVLSHGEHP-------PLSHLLWPCLSALDAHHCRASVRLCGQDESSLSEFYSLALN---RLGYPET-PSYA-HAQLPGLTGQSSMSLSDPD--------SAILALESAASLKKKVKKMFCEPGNVDRCPPLDCLRLLLLPFKRLSGSEGDSIRVSRSAELGGDLVFASFDELKSAFVHKSLHPGDLKAHVESGLLEMQNR-----LRELIGPELTKKLDGAFAAPDKKSAGAAADELNPHRLDIRVGRILDV 737
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A267H0L6 (Tyrosine--tRNA ligase (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021276g1 PE=3 SV=1) HSP 1 Score: 479.559 bits (1233), Expect = 2.188e-151 Identity = 299/766 (39.03%), Postives = 448/766 (58.49%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPV---KSQLNIDLLKK-YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGK-ENLDQEAYPIET-----------EKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPG-SPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGS---DYQLSREYALLLYQSFSHVTERDCSEMLLQMI--GQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWP----SFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVK----SALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSERKHKFKHDKNAFNPNSLNIRVARIVSI 2288
++ RNLQE +G ++L K++ ++YWGTA TGRPH+AYFVPM+K+AD+L AGC VT+LFADLHAYLDNMKAPW LL KRT YYE +IKGMLK++ VPLD+L+FVRGT+++L + YT +VY+I + HDA KAG +VVKQ++ PLISG++YP LQALDE++L DAQFGGVDQRKIFM AEK L +LG++KR HLMNPMIPGLTGGKMSASE +SKIDLLD+A+SVS K++ A C+ ++ NG+L+F+++V+ P+ L I KK Y +++ DF + L LK V +L L+G +++EF +S + + L +AYP + + D+ P +LA + +L +F S D+ P G SP++LW++R G H+G+L+PL +++ W++T+L+ ++ A L + + + RG K +S T D + S + +++Y L +Y+ S VT + + ++ G+HP LS L++PCLS LD + +VRL A L+ L +P + P + H + L G ++M D ++I +SA ++ +K+KK F +P ++DR P L+ L L+ P S + + V R+ E GGD+ ++S D+++ V LHPGD+K S L+E N ++ L+G +L + L AF + K + NP+ L+IRV RI+ +
Sbjct: 10 QLIIRNLQEVVGLDRLKAKTAKHE-LSVYWGTATTGRPHVAYFVPMSKLADYLLAGCHVTVLFADLHAYLDNMKAPWSLLNKRTNYYETVIKGMLKSIGVPLDRLKFVRGTDFELGKEYTRDVYRIAAEVTVHDAKKAGAEVVKQVASPLISGLLYPCLQALDEQYLGVDAQFGGVDQRKIFMLAEKYLVNLGYSKRIHLMNPMIPGLTGGKMSASEADSKIDLLDSAESVSAKVAGAQCSPGAAEGNGVLSFLQYVVFPLLGGTDGLAIGSSKKRYAEFQQVSEDFASGSLTAEDLKGCVADSLNRLLGPVRKEFEESSELQQLLVDAYPKDQPAKSSSGGDAGDVADRLPGIQLATIDAKLIESLSRFATQFG-----SQDSVPVKSGSSPRLLWTIRPCGPMHLGHLVPLLGALSMAKAGWRVTLLVSEMQAFL----DRKRAPIDSLEVRGSTVKDVLSRLATGLLGDTAAAAVTVTSGLEELSYNKDYVLDMYRLVSLVTVDEAMQAGSGVLSHGEHP-------PLSHLLWPCLSALDAHHCRASVRLCGQDESSLSEFSSLA-LNC-LGYP-ATPSFA-HAQLPGLTGQSSMSLSDPD--------SAILALESAASLKKKVKKMFCEPGNVDRCPPLDCLRLLLLPFKRLSGSEGDSIRVSRSAELGGDLVFASFDELKSAFVDKSLHPGDLKAHVESGLLEMQNR-----LRELIGPELTKKLDGAFAAPDKKSAGAAADELNPHRLDIRVGRILDV 741
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A1I8IVH2 (Tyrosine--tRNA ligase OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 479.945 bits (1234), Expect = 9.899e-149 Identity = 301/767 (39.24%), Postives = 450/767 (58.67%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPV---KSQLNIDLLKK-YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGK-ENLDQEAYPIET-----------EKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPG-SPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGS---DYQLSREYALLLYQSFSHVTERDCSEMLLQMI--GQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVA-DYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWP----SFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVK----SALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSERKHKFKHDKNAFNPNSLNIRVARIVSI 2288
++ RNLQE +G ++L K++ ++YWGTA TGRPH+AYFVPM+K+AD+L AGC VT+LFADLHAYLDNMKAPW LL KRT YYE +IKGMLK++ VPLD+L+FVRGT+++L + YT +VY+I + HDA KAG +VVKQ++ PLISG++YP LQALDE++L DAQFGGVDQRKIFM AEK L +LG++KR HLMNPMIPGLTGGKMSASE +SKIDLLD+A+SVS K++ A C+ ++ NG+L+F+++V+ P+ L I KK Y +++ DF + L LK V +L L+G +++EF +S + + L +AYP + + D+ P +LA + +L +F S D+ P G SP++LW++R G H+G+L+PL +++ W++T+L+ ++ A L + + + RG K +S T D + S + +++Y L +Y+ S VT + + ++ G+HP LS L++PCLS LD + +VRL D S + F A L +P + P + H + L G ++M D ++I +SA ++ +K+KK F +P ++DR P L+ L L+ P S + + V R+ E GGD+ ++S D+++ V LHPGD+K S L+E N ++ L+G +L + L AF + K + NP+ L+IRV RI+ +
Sbjct: 332 QLIIRNLQEVVGLDRLKAKTAKHE-LSVYWGTATTGRPHVAYFVPMSKLADYLLAGCHVTVLFADLHAYLDNMKAPWSLLNKRTNYYETVIKGMLKSIGVPLDRLKFVRGTDFELGKEYTRDVYRIAAEVTVHDAKKAGAEVVKQVASPLISGLLYPCLQALDEQYLGVDAQFGGVDQRKIFMLAEKYLVNLGYSKRIHLMNPMIPGLTGGKMSASEADSKIDLLDSAESVSAKVAGAQCSPGAAEGNGVLSFLQYVVFPLLGGTDGLAIGSSKKRYAEFQQVSEDFASGSLTAEDLKGCVADSLNRLLGPVRKEFEESSELQQLLVDAYPKDQPAKSSSGGDAGDVADRLPGIQLATIDAKLIESLSRFATQFG-----SQDSVPVKSGSSPRLLWTIRPCGPMHLGHLVPLLGALSMAKAGWRVTLLVSEMQAFL----DRKRAPIDSLEVRGSTVKDVLSRLATGLLGDTAAAAVTVTSGLEELSYNKDYVLDMYRLVSLVTVDEAMQAGSGVLSHGEHP-------PLSHLLWPCLSALDAHHCRASVRLCGQDESSLSEFSSLALNC---LGYP-ATPSFA-HAQLPGLTGQSSMSLSDPD--------SAILALESAASLKKKVKKMFCEPGNVDRCPPLDCLRLLLLPFKRLSGSEGDSIRVSRSAELGGDLVFASFDELKSAFVDKSLHPGDLKAHVESGLLEMQNR-----LRELIGPELTKKLDGAFAAPDKKSAGAAADELNPHRLDIRVGRILDV 1063
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A1I8G1E4 (Tyrosine--tRNA ligase OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 471.47 bits (1212), Expect = 1.387e-148 Identity = 298/762 (39.11%), Postives = 446/762 (58.53%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPV---KSQLNIDLLKK-YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSI------SLDTFPDIPG-SPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGS---DYQLSREYALLLYQSFSHVTERDCSEMLLQMI--GQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSA--RLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWP----SFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVK----SALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSERKHKFKHDKNAFNPNSLNIRVARIVSI 2288
++ RNLQE +G ++L K++ ++YWGTA TGRPH+AYFVPM+K+AD+L AGC VT+LFADLHAYLDNMKAPW LL KRT YYE +IKGMLK++ VPLD+L+FVRGT+++L + YT +VY+I + HDA KAG +VVKQ++ PLISG++YP LQALDE++L DAQFGGVDQRKIFM AEK L +LG+AKR HLMNPMIPGLTGGKMSASE +SKIDLLD+A+SVS K++ A C+ ++ NG+L+F+++V+ P+ L I KK Y +++ DF + L LK V +L L+G +++EF +S E +++ + ++A+ ++G Q L ++ + ++LS S D+ P G SP++LW++R +G H+G+L+PL +++ WK+T+L+ ++ A L + + + RG K +S T D + S + +++Y L +Y+ S VT + + ++ G+HP LS L++PCLS LD + +VRL D A RL +P++ P Y H + L GL++M D ++I +SA ++ +K P ++DR P L+ L L+ P S + + V R+ E GGD+ ++S D+++ V LHPGD+K S L+E N ++ L+G +L + L AF K + NP+ L+IRV RI+ +
Sbjct: 6 QLIIRNLQEVVGLDRLKAKTAKHE-LSVYWGTATTGRPHVAYFVPMSKLADYLLAGCHVTVLFADLHAYLDNMKAPWSLLNKRTNYYETVIKGMLKSIGVPLDRLKFVRGTDFELGKEYTRDVYRIAAEVTVHDAKKAGAEVVKQVASPLISGLLYPCLQALDEQYLGVDAQFGGVDQRKIFMLAEKYLVNLGYAKRIHLMNPMIPGLTGGKMSASEADSKIDLLDSAESVSAKVAGAQCSPGAAEGNGVLSFLQYVVFPLLGGTDGLAIGSSKKRYAEFQQVSEDFASGSLTAEDLKGCVADSLNRLLGPVRKEFEESS-----------ELQQLLRWGCGDVADRLAGIQ--LATIDAKLIESLSRFATQFGSQDSVPVKSGSSPRLLWTIRPSGPMHLGHLVPLIGALSMAKAGWKVTLLVSEMQAFL----DRKRAPIDSLEVRGSTVKDVLSRLATGLLGDTAAAAVTVTSGLEELSYNKDYVLDMYRLVSLVTVDEAMQAGSGVLSHGEHP-------PLSHLLWPCLSALDAHHCRASVRLCGQ----DESSLSEFSSLALNRLGYPET-PSYA-HAQLPGLTGLSSMSLSDPD--------SAILALESAASLKKK-------PGNVDRCPPLDCLRLLLLPFKRLSGSEGDSIRVSRSAELGGDLVFASFDELKSAFVDKFLHPGDLKAHVESGLLEMQNR-----LRELIGPELTKKLDGAFAEPDKKSAGAAADELNPHRLDIRVGRILDV 716
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A5C6NE45 (Tyrosine--tRNA ligase OS=Takifugu flavidus OX=433684 GN=D4764_21G0002750 PE=3 SV=1) HSP 1 Score: 471.47 bits (1212), Expect = 4.807e-148 Identity = 299/791 (37.80%), Postives = 447/791 (56.51%), Query Frame = 3
Query: 78 MNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDL------LKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIET-------------EKIDQNPPTELANTISGYQNNLK----------SLLE---EFPQALSISLDTFPDIPGSPK-----------ILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSE-------RKHKFKHDKNAFNPNSLNIRVARIVSIE 2291
+++TRNLQE +G EKL Q + + + LYWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPL+KL+FV+GT++QL+ V ++ +T HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KRAHLMNPM+PGLTG KMS+SEE SKIDLLD+ + V KL A C + NG+L+F+K+V+ P+ + I K Y EE+E DF ++ + LK+ VETAL L+ ++++F L AYP +T E + PP + SG++ + S++E Q + ++ G K + W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L ++F+ G+D+QLSR+Y L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P H + GLT K ++ + + I + DS + + +KLKKAF +P ++ + L+ ++++++P + F +KR + GGD Y+ +++ D + +HPGD+K+++ LN + PI K +L +L A+PS+ K + + P+ L+IRV +I+S+E
Sbjct: 11 FHLITRNLQEVLGEEKLKQIL-QERELRLYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQIIKAMLESIGVPLEKLKFVKGTDFQLSRC----VPTVLRVTE-HDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYDKRAHLMNPMVPGLTGAKMSSSEEESKIDLLDSKEDVKKKLKKAFCEPGNIQNNGVLSFVKYVLFPLCGEFRIKRDPKWGGDKTYTEFEEVEKDFADETVHPGDLKASVETALNLLLDPIRKKFESPELRKLTNSAYPSKTKAAVKGAKGGGGAEDDELVPP----DWTSGWERSSAWRRWDIIIGLSIMEGQLTPDQKFHLITRNLQEVLGEEKLKQILQERELRLYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQIIKAMLESIGVPLEKLKFVKGTDFQLSRQYTLDVYRLSSVVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYDKRAHLMNPMVPGLTGAKM------SSSEEESKIDLLDSKEDVKKKLKKAFCEPGNIQNNGVLSFVKYVLFPLCGE---FRIKRDPKWGGDKTYTEFEEVEKDFADETVHPGDLKASVETALN-LLLDPIRKKFESPELRELTNSAYPSKTKAAVKGAKGGGGAEDDELVPSRLDIRVGKIISVE 763
BLAST of Tyrosine--tRNA ligase vs. Ensembl Cavefish
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:103038774]) HSP 1 Score: 371.318 bits (952), Expect = 7.603e-118 Identity = 184/336 (54.76%), Postives = 240/336 (71.43%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKK------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++ N++TRNLQE +G EKL Q + + + +YWGTA TG+PH+AYFVPM+KIADFL+AGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPLDKL+FV+GT+YQL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KR H+MNPM+PGLTG KMS+SEE SKIDLLD + V KL A C + NG+L+F+K V+ P+ S+ I K Y + EE+E F + + LK+ VE AL LM ++++F + L AYP
Sbjct: 8 DEKFNLITRNLQEVLGEEKLKQVL-QERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVQYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMITEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYTKRIHMMNPMVPGLTGTKMSSSEEESKIDLLDKKEDVKKKLKKAFCEPGNIQNNGVLSFVKHVLFPLNSEFVIKRDPKWGGDQVYTSFEEVEKHFAEEQIHPGDLKASVEVALNKLMDPIRKKFEMPALKKLTNSAYP 342 HSP 2 Score: 149.828 bits (377), Expect = 1.008e-37 Identity = 115/375 (30.67%), Postives = 197/375 (52.53%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDF--AAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSERKHKFKHDKNAFN---------PNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R Q Y+Q I + L + L ++F+ G+DYQLSREY L +Y+ S +TE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A K L + K +++ + + L G T M S + ++K I + D + + +KLKKAF +P ++ + L+ ++H+++P + F++KR + GGD Y+S +++ + +HPGD+K+++ LN + + PI K L++L A+P K+KF N N P+ L+IRV +I+S+E
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNM----KAPWELLELRVQYYEQVIKAMLESIGVPLDKLKFVKGTDYQLSREYTLDVYRLSSMITEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTLAEKYLPSLGYTKR--IHMMNPMVPGLTG-TKMSSSEEESK--------IDLLDKKEDVKKKLKKAFCEPGNIQNNGVLSFVKHVLFPL---NSEFVIKRDPKWGGDQVYTSFEEVEKHFAEEQIHPGDLKASVEVALN-KLMDPIRKKFEMPALKKLTNSAYPEPSKNKFATKGNPKNAAAEEDDIIPSRLDIRVGKIISVE 381
BLAST of Tyrosine--tRNA ligase vs. Ensembl Yeast
Match: TYS1 (Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; human ortholog YARS functionally complements the heat sensitivity of a ts allele; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000003417]) HSP 1 Score: 343.969 bits (881), Expect = 3.499e-110 Identity = 166/350 (47.43%), Postives = 245/350 (70.00%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCID-KNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLT-GGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKS--------QLNIDLLKK------YNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGK-ENLDQEAYPIET 1067
N+ ++T+NLQE + + + ++ + ++ LYWGTAPTGRPH YFVPMTK+ADFL+AGC VT+L ADLHA+LDNMKAP E++ R +YYE+ IK +L+++ VP++KL+FV G+ YQL +YT +++++ I S +DA +AG DVVKQ+++PL+SG+IYPL+QALDE+FL+ D QFGGVDQRKIF+ AE+ L LG+ KRAHLMNPM+PGL GGKMSAS+ NSKIDLL+ V K+++A C+ + NG+L+F+++VI P++ + ID +K Y + EE++L F + L LK V A+ L+ +++EF + + + ++ YP+ T
Sbjct: 10 NEAFGLITKNLQEVLNPQIIKDVLEVQKRHLKLYWGTAPTGRPHCGYFVPMTKLADFLKAGCEVTVLLADLHAFLDNMKAPLEVVNYRAKYYELTIKAILRSINVPIEKLKFVVGSSYQLTPDYTMDIFRLSNIVSQNDAKRAGADVVKQVANPLLSGLIYPLMQALDEQFLDVDCQFGGVDQRKIFVLAEENLPSLGYKKRAHLMNPMVPGLAQGGKMSASDPNSKIDLLEEPKQVKKKINSAFCSPGNVEENGLLSFVQYVIAPIQELKFGTNHFEFFIDRPEKFGGPITYKSFEEMKLAFKEEKLSPPDLKIGVADAINELLEPIRQEFANNKEFQEASEKGYPVAT 359 HSP 2 Score: 98.5969 bits (244), Expect = 2.379e-22 Identity = 73/322 (22.67%), Postives = 148/322 (45.96%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDF--AAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWP----SFRDSE-RFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIK 2153
K+ W TG PH GY +P+ KLA+ ++T+L+ D+ A L + K ++ R + Y+ I + L + + ++F+ GS YQL+ +Y + +++ + V++ D ++ Q N LS L+YP + LD+ L + + +D+ + F A + L + K + LA G + P + I + + + +K+ AF P +++ + L+ ++++I P F + F + R + GG + Y S +++++ + L P D+K + + +NE ++PI+
Sbjct: 41 KLYWGTAPTGRPHCGYFVPMTKLADFLKAGCEVTVLLADLHAFLDNM----KAPLEVVNYRAKYYELTIKAILRSINVPIEKLKFVVGSSYQLTPDYTMDIFRLSNIVSQNDAKRAGADVVKQVANPL-----LSGLIYPLMQALDEQFLD----VDCQFGGVDQRKIFVLAEENLPSLGYKKRAHLMNPMVPGLAQGGKMSASDP----------NSKIDLLEEPKQVKKKINSAFCSPGNVEENGLLSFVQYVIAPIQELKFGTNHFEFFIDRPEKFGGPITYKSFEEMKLAFKEEKLSPPDLKIGVADAINE-LLEPIR 338
BLAST of Tyrosine--tRNA ligase vs. Ensembl Nematostella
Match: EDO32430 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV20]) HSP 1 Score: 363.229 bits (931), Expect = 3.494e-115 Identity = 187/340 (55.00%), Postives = 241/340 (70.88%), Query Frame = 3
Query: 81 NIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPV------------KSQLNID--LLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
++TRNLQE IG ++L + + K +N LYWGTA TGRPH+AYFVPMTKIADFL AGC VTIL ADLHAYLDN+KAPWELL+ R +YYE LIK ML+++ VPLDKL+FV+GTEYQLN++Y +V+K+ I + HD KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF +AEK L +LG+ KR HLMNPM+PGLTGGKMS+SEE+SKID+LD+A V K+ A C E + NG+LAF KFV+ P+ +S+ N + Y+T+E+ F K L LK+ V L LM ++++F + + AYP
Sbjct: 17 QLITRNLQEVIGDDRL-KVLLKTQNLKLYWGTATTGRPHVAYFVPMTKIADFLHAGCEVTILLADLHAYLDNLKAPWELLEHRVKYYEELIKAMLESINVPLDKLKFVKGTEYQLNKDYILDVFKMTTIATEHDCKKAGAEVVKQVQHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPNLGYQKRIHLMNPMVPGLTGGKMSSSEEDSKIDMLDSASVVKKKVKKAFCEEGNIDDNGVLAFAKFVLFPILELKNQKEFVIERSEENGGNLVFADYSTLEKT---FAEKDLHPLDLKNGVCKFLDQLMDPVRKKFEQPEFLKIKNLAYP 352 HSP 2 Score: 142.124 bits (357), Expect = 1.716e-35 Identity = 108/391 (27.62%), Postives = 204/391 (52.17%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIG--QHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFR--DFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWP--SFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSERKHKFK-----HDKNAFNPNSLNIRVARIVSIEY---VDRTYQIQLRSGLE 2336
K+ W TG PH+ Y +P+ K+A+ H ++TIL+ D+ A+L +L P W L+ R + Y++ I + L + L ++F+ G++YQL+++Y L +++ + TE DC + +++ QHP LS L+YP L LD+ LK + A + +D+ + FA K L + K +++ + + L G M S + D+K I M DSA + +K+KKAF + ++D + L + +++P ++ + F+++R+ ENGG++ ++ + LHP D+K+ + ++L++ + P+ K + ++ LA+P +K K K + + A +P+ L++RV ++VS + D Y Q+ G E
Sbjct: 42 KLYWGTATTGRPHVAYFVPMTKIADFLHAGCEVTILLADLHAYL---DNLKAP-WELLEHRVKYYEELIKAMLESINVPLDKLKFVKGTEYQLNKDYILDVFKMTTIATEHDCKKAGAEVVKQVQHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTFAEKYLPNLGYQKR--IHLMNPMVPGLTG-GKMSSSEEDSK--------IDMLDSASVVKKKVKKAFCEEGNIDDNGVLAFAKFVLFPILELKNQKEFVIERSEENGGNLVFADYSTLEKTFAEKDLHPLDLKNGVCKFLDQ-LMDPVRKKFEQPEFLKIKNLAYPPPKKTKTKGGGSANAERAVDPSRLDLRVGKVVSAKKHPDADALYLEQVDVGEE 405
BLAST of Tyrosine--tRNA ligase vs. Ensembl Medaka
Match: yars1 (tyrosyl-tRNA synthetase 1 [Source:NCBI gene;Acc:101169468]) HSP 1 Score: 373.629 bits (958), Expect = 1.242e-118 Identity = 180/336 (53.57%), Postives = 239/336 (71.13%), Query Frame = 3
Query: 69 NDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDL------LKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYP 1058
+ ++TRNLQE +G EK+ Q + + + +YWGTA TG+PH+AYFVPM+KIADFLRAGC VTILFADLHAYLDNMKAPWELL+ R +YYE +IK ML+++ VPL+KL F++GT++QL+ YT +VY++ + + HDA KAG +VVKQ+ PL+SG++YP LQALDEE+L DAQFGGVDQRKIF AEK L LG+ KRAHLMNPM+PGLTG KMS+SEE SKIDLLD+ + V KL A C + NG+L+F+K V+ P+ + +I K Y+ E++E DF + + LK+ VE AL L+ ++++F L AYP
Sbjct: 8 EEKFQLITRNLQEVLGEEKVKQIL-QERELKVYWGTATTGKPHVAYFVPMSKIADFLRAGCEVTILFADLHAYLDNMKAPWELLELRVKYYEQVIKAMLESIGVPLEKLHFIKGTDFQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTLAEKYLPSLGYVKRAHLMNPMVPGLTGAKMSSSEEESKIDLLDSKEDVKKKLKKAFCEPGNIQNNGVLSFVKHVLFPLHGEFSIKRDPKWGGDKTYSVFEDVEKDFAEERVHPGDLKASVEAALNQLLDPIRKKFQSPELRKLTNSAYP 342 HSP 2 Score: 145.976 bits (367), Expect = 2.653e-36 Identity = 111/377 (29.44%), Postives = 188/377 (49.87%), Query Frame = 3
Query: 1209 KILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQ--HPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPI-KSXXXXXXXXXXXXAFPSERKHKF-------------KHDKNAFNPNSLNIRVARIVSIE 2291
K+ W TG+PH+ Y +P+ K+A+ ++TIL D+ A+L + K W L+ R + Y+Q I + L + L + FI G+D+QLSREY L +Y+ S VTE D + +++ Q HP LS L+YP L LD+ LK + A + +D+ + F A + P V H + GLT K ++ + + I + DS + + +KLKKAF +P ++ + L+ ++H+++P + F +KR + GGD YS + + D + +HPGD+K+++ LN+ + PI K +L +L A+P K K + + P+ L+IRV +++S+E
Sbjct: 37 KVYWGTATTGKPHVAYFVPMSKIADFLRAGCEVTILFADLHAYLDNM----KAPWELLELRVKYYEQVIKAMLESIGVPLEKLHFIKGTDFQLSREYTLDVYRLSSMVTEHDAKKAGAEVVKQVEHP-------LLSGLLYPGLQALDEEYLK----VDAQFGGVDQRKIFTL---AEKYLPSLGYVKRAHLMNPMVPGLTGAKM------SSSEEESKIDLLDSKEDVKKKLKKAFCEPGNIQNNGVLSFVKHVLFPLHGE---FSIKRDPKWGGDKTYSVFEDVEKDFAEERVHPGDLKASVEAALNQ-LLDPIRKKFQSPELRKLTNSAYPDPSKTKMGGKGAKAAAGGGGGGEDDELAPSRLDIRVGKVISVE 385
BLAST of Tyrosine--tRNA ligase vs. Planmine SMEST
Match: SMESG000055897.1 (SMESG000055897.1) HSP 1 Score: 1754.57 bits (4543), Expect = 0.000e+0 Identity = 875/883 (99.09%), Postives = 879/883 (99.55%), Query Frame = 3
Query: 75 MMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSERKHKFKHDKNAFNPNSLNIRVARIVSIEYVDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCLLVVKPSTNIDHSRALGMLHLVANGDGGDCSNAXXXXXXXXXXHPYNDNVSFENHEIICPHLKENDENVIASQIATGLAVVNGSLQWKIDGSLARDIELIGDGACQKIMIG 2723
MMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDI+GNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDT SISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSLLGKDLEQLLLLAFP E+KHKFKHDK+AFNPNSLNIRVARIVSIEYVDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCLLVVKPSTNIDHSRALGMLH VANGDGGDCSNALPLPLKLVKL PYNDNVSFENHEIICPHLKENDENVIASQIATGLAVVNGSLQWKIDGSLARDIELIGDGACQKI+IG
Sbjct: 1 MMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDIKGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTISISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSLLGKDLEQLLLLAFPLEQKHKFKHDKDAFNPNSLNIRVARIVSIEYVDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCLLVVKPSTNIDHSRALGMLHRVANGDGGDCSNALPLPLKLVKLQPYNDNVSFENHEIICPHLKENDENVIASQIATGLAVVNGSLQWKIDGSLARDIELIGDGACQKIVIG 883
BLAST of Tyrosine--tRNA ligase vs. Planmine SMEST
Match: SMESG000055897.1 (SMESG000055897.1) HSP 1 Score: 1752.26 bits (4537), Expect = 0.000e+0 Identity = 874/882 (99.09%), Postives = 878/882 (99.55%), Query Frame = 3
Query: 78 MNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSERKHKFKHDKNAFNPNSLNIRVARIVSIEYVDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCLLVVKPSTNIDHSRALGMLHLVANGDGGDCSNAXXXXXXXXXXHPYNDNVSFENHEIICPHLKENDENVIASQIATGLAVVNGSLQWKIDGSLARDIELIGDGACQKIMIG 2723
MNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDI+GNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDT SISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSLLGKDLEQLLLLAFP E+KHKFKHDK+AFNPNSLNIRVARIVSIEYVDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCLLVVKPSTNIDHSRALGMLH VANGDGGDCSNALPLPLKLVKL PYNDNVSFENHEIICPHLKENDENVIASQIATGLAVVNGSLQWKIDGSLARDIELIGDGACQKI+IG
Sbjct: 1 MNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDIKGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTISISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSLLGKDLEQLLLLAFPLEQKHKFKHDKDAFNPNSLNIRVARIVSIEYVDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCLLVVKPSTNIDHSRALGMLHRVANGDGGDCSNALPLPLKLVKLQPYNDNVSFENHEIICPHLKENDENVIASQIATGLAVVNGSLQWKIDGSLARDIELIGDGACQKIVIG 882
BLAST of Tyrosine--tRNA ligase vs. Planmine SMEST
Match: SMESG000068024.1 (SMESG000068024.1) HSP 1 Score: 1566.98 bits (4056), Expect = 0.000e+0 Identity = 769/882 (87.19%), Postives = 821/882 (93.08%), Query Frame = 3
Query: 78 MNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWELLQKRTEYYEILIKGMLKALFVPLDKLRFVRGTEYQLNENYTANVYKIIGITSTHDATKAGCDVVKQISDPLISGIIYPLLQALDEEFLNADAQFGGVDQRKIFMYAEKCLGHLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTADSVSYKLSTANCTELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIELDFNNKLLDENSLKSIVETALRSLMGNLKEEFIKSGKENLDQEAYPIETEKIDQNPPTELANTISGYQNNLKSLLEEFPQALSISLDTFPDIPGSPKILWSVRATGEPHIGYLLPLRKLANLSHRSWKITILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISSALTNYQADLSNIEFIFGSDYQLSREYALLLYQSFSHVTERDCSEMLLQMIGQHPNSSDIRGNLSSLVYPCLSVLDQLILKPTVRLVADYSYIDRFRDFAAKLSARLHFPKSNPVYIRHETILALNGLTAMKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSERKHKFKHDKNAFNPNSLNIRVARIVSIEYVDRTYQIQLRSGLEEFLVRRSQIGSWTPNDLCLLVVKPSTNIDHSRALGMLHLVANGDGGDCSNAXXXXXXXXXXHPYNDNVSFENHEIICPHLKENDENVIASQIATGLAVVNGSLQWKIDGSLARDIELIGDGACQKIMIG 2723
MN+VTRNLQECIG EKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPW+LLQKRTEYYEILIKGMLKAL VPLDKLRF+RGTEYQL+ENYTA+VYKIIGIT++HDA+KAGC+VVKQ SDPLISGIIYPLLQALDEEFLN DAQFGGVDQRKIFMYAEKCLG+LGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDT D+VS KLSTANC+ELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIE DF NK+LDENSLK+IVETALRSLM +LKEEF++SG ENLDQEAYPIETE+I+ NPP EL NTISGY N+LKSLLEEFPQ S SLD FPDIP SPKILWSVRATGEPHIGYLLPLRKLANLSHR+WK+TILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFIS ALTNYQA+LSNI+ IFGSDYQLSREYALLLYQSFSH+TERDCSEML QMIGQHPNSSDI GNLSSLVYPCLSVLDQL LKPTVRLVADY +I +FR+FA++LSA LHFP+SNPV+IRHETILALNG TAMKSPKI TK AP D SISMCDS D IARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRD E FLVKRT+ENGG+V+YSS+DQIR+DL+GD++HPGDVKSALVEYLNERFIQPIKS +G++L + L AFP E KHKFK +K AFNPNSLNIRVARIVSIEY DR YQI+LRSGLEE+ V+RSQ+GSWTPNDLCLLVVKPSTNID SRALGMLH VANG+ GD SN+LP PLKLVK YNDNVSFENHEIICPHLKENDENVI SQIATGLA VNGSLQWKIDGSLARDIELIGD AC KI+IG
Sbjct: 1 MNVVTRNLQECIGAEKLDQCIDKNKNFNLYWGTAPTGRPHIAYFVPMTKIADFLRAGCSVTILFADLHAYLDNMKAPWDLLQKRTEYYEILIKGMLKALSVPLDKLRFIRGTEYQLHENYTASVYKIIGITTSHDASKAGCEVVKQTSDPLISGIIYPLLQALDEEFLNVDAQFGGVDQRKIFMYAEKCLGNLGFAKRAHLMNPMIPGLTGGKMSASEENSKIDLLDTPDAVSLKLSTANCSELSSSTNGILAFIKFVILPVKSQLNIDLLKKYNTIEEIEFDFINKILDENSLKAIVETALRSLMEHLKEEFVQSGHENLDQEAYPIETEEIESNPPNELINTISGYPNHLKSLLEEFPQTSSSSLDEFPDIPRSPKILWSVRATGEPHIGYLLPLRKLANLSHRNWKVTILIEDIGAHLFSQHSLNKPAWNLIRQRGQLYKQFISGALTNYQANLSNIDIIFGSDYQLSREYALLLYQSFSHITERDCSEMLQQMIGQHPNSSDITGNLSSLVYPCLSVLDQLTLKPTVRLVADYPHIAQFRNFASQLSASLHFPRSNPVFIRHETILALNGSTAMKSPKIGTKTAPKDIISISMCDSVDAIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDLETFLVKRTDENGGNVEYSSIDQIRIDLIGDIMHPGDVKSALVEYLNERFIQPIKSQIGENLGKFLFAAFPLEPKHKFKLNKKAFNPNSLNIRVARIVSIEYADRVYQIRLRSGLEEYSVKRSQVGSWTPNDLCLLVVKPSTNIDQSRALGMLHRVANGNTGDFSNSLPFPLKLVKQQ-YNDNVSFENHEIICPHLKENDENVIVSQIATGLAEVNGSLQWKIDGSLARDIELIGDNACVKIVIG 881
BLAST of Tyrosine--tRNA ligase vs. Planmine SMEST
Match: SMESG000019862.1 (SMESG000019862.1) HSP 1 Score: 236.884 bits (603), Expect = 4.830e-73 Identity = 129/134 (96.27%), Postives = 131/134 (97.76%), Query Frame = 3
Query: 1809 MKSPKIDTKAAPTDTTSISMCDSADTIARKLKKAFFDPKSLDRDPSLNILEHLIWPSFRDSERFLVKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSXXXXXXXXXXXXAFPSER 2210
MKSPKIDTKAAPTDT SISMCDSADTIARKLKKAFFD KSLDRDPSLNILEHLIWPSFRDSERFL+KRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSLLGKDLEQLLLLAFP E+
Sbjct: 1 MKSPKIDTKAAPTDTISISMCDSADTIARKLKKAFFDQKSLDRDPSLNILEHLIWPSFRDSERFLMKRTNENGGDVKYSSVDQIRVDLVGDLLHPGDVKSALVEYLNERFIQPIKSLLGKDLEQLLLLAFPLEQ 134 The following BLAST results are available for this feature:
BLAST of Tyrosine--tRNA ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of Tyrosine--tRNA ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of Tyrosine--tRNA ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Tyrosine--tRNA ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Tyrosine--tRNA ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Tyrosine--tRNA ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Tyrosine--tRNA ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Tyrosine--tRNA ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Tyrosine--tRNA ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Tyrosine--tRNA ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021122 ID=SMED30021122|Name=Tyrosine--tRNA ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=2833bpback to top protein sequence of SMED30021122-orf-1 >SMED30021122-orf-1 ID=SMED30021122-orf-1|Name=SMED30021122-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=892bp MSFNFKNDMMNIVTRNLQECIGTEKLDQCIDKNKNFNLYWGTAPTGRPHIback to top Annotated Terms
The following terms have been associated with this transcript:
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