SMED30021087

Overview
NameSMED30021087
Smed IDSMED30021087
Length (bp)676
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30021087 (SMED30021087) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30021087 (SMED30021087) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30021087 (SMED30021087) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30021087 (SMED30021087) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30021087

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30021087SMESG000014860.1 SmedASXL_021433SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30021087SMESG000014860.1 dd_Smed_v4_6894_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021087SMESG000014860.1 dd_Smed_v4_6894_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021087SMESG000014860.1 dd_Smed_v4_6894_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30021087SMESG000014860.1 dd_Smed_v6_6894_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30021087SMESG000014860.1 dd_Smed_v4_6894_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30021087 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000878:93046..97556 -3309
Homology
[Close]
BLAST of SMED30021087 vs. TrEMBL
Match: A0A3Q0KHA4 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 4.844e-9
Identity = 39/107 (36.45%), Postives = 56/107 (52.34%), Query Frame = 2 
Query:  203 FGSIKRILNKRPLFTCRPFGSEDE--ICCGCCASMNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSP----CESLYCDNFRCCCFMC 505
            +  +K IL KR   + +P  ++ +   CC CC S+ D C C    S D CL  ICPQRKTH F D++LC+    R     + + +      C S YC++   CCF+C
Sbjct:   58 YAIVKSILAKR-TPSVKPSANQLQPFACCPCCLSVADLCNCCRVTSIDACLNGICPQRKTHDFTDLILCQICLGRPGRMKSAVPLVKCPVCCRSAYCEDTVICCFLC 163
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BLAST of SMED30021087 vs. TrEMBL
Match: G4LWR8 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_068750 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 6.906e-9
Identity = 39/107 (36.45%), Postives = 56/107 (52.34%), Query Frame = 2 
Query:  203 FGSIKRILNKRPLFTCRPFGSEDE--ICCGCCASMNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSP----CESLYCDNFRCCCFMC 505
            +  +K IL KR   + +P  ++ +   CC CC S+ D C C    S D CL  ICPQRKTH F D++LC+    R     + + +      C S YC++   CCF+C
Sbjct:   69 YAIVKSILAKRTP-SVKPSANQLQPFACCPCCLSVADLCNCCRVTSIDACLNGICPQRKTHDFTDLILCQICLGRPGRMKSAVPLVKCPVCCRSAYCEDTVICCFLC 174
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BLAST of SMED30021087 vs. TrEMBL
Match: A0A183NF97 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 PE=4 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.931e-8
Identity = 40/108 (37.04%), Postives = 57/108 (52.78%), Query Frame = 2 
Query:  203 FGSIKRIL-NKRPLFTCRPFGSEDE--ICCGCCASMNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSP----CESLYCDNFRCCCFMC 505
            +  +K IL N+ P    +P  S+ +   CC CC S+ D C C    S D CL  ICPQRKTH F D++LC+    R     + + +      C+S YC++   CCF+C
Sbjct:   54 YAIVKSILANRTP--NVKPSVSQLQPFACCPCCLSVADLCNCCRVTSIDACLNGICPQRKTHDFTDLLLCQICLGRPGRMKSTVPLVKCPVCCQSAYCEDTVICCFLC 159
[Close]
BLAST of SMED30021087 vs. TrEMBL
Match: A0A3P7YKZ6 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS783 PE=4 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.970e-8
Identity = 40/108 (37.04%), Postives = 57/108 (52.78%), Query Frame = 2 
Query:  203 FGSIKRIL-NKRPLFTCRPFGSEDE--ICCGCCASMNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSP----CESLYCDNFRCCCFMC 505
            +  +K IL N+ P    +P  S+ +   CC CC S+ D C C    S D CL  ICPQRKTH F D++LC+    R     + + +      C+S YC++   CCF+C
Sbjct:   54 YAIVKSILANRTP--NVKPSVSQLQPFACCPCCLSVADLCNCCRVTSIDACLNGICPQRKTHDFTDLLLCQICLGRPGRMKSTVPLVKCPVCCQSAYCEDTVICCFLC 159
[Close]
BLAST of SMED30021087 vs. TrEMBL
Match: A0A183JBL7 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.970e-8
Identity = 39/108 (36.11%), Postives = 57/108 (52.78%), Query Frame = 2 
Query:  203 FGSIKRIL-NKRPLFTCRPFGSEDEI--CCGCCASMNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSP----CESLYCDNFRCCCFMC 505
            +  +K IL N+ P    +P  ++ ++  CC CC S+ D C C    S D CL  ICPQRKTH F D++LC+    R     + + +      C S YC++   CCF+C
Sbjct:   54 YAIVKSILANRTP--NVKPSVNQLQLFACCPCCLSVADLCNCCRVTSIDACLNGICPQRKTHDFTDLLLCQICLGRPGRMKSTVPLVKCPVCCRSAYCEDTVICCFLC 159
[Close]
BLAST of SMED30021087 vs. Planmine SMEST
Match: SMESG000014860.1 (SMESG000014860.1)

HSP 1 Score: 184.111 bits (466), Expect = 3.281e-60
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 2 
Query:  299 MNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSPCESLYCDNFRCCCFMCMCHKELPVTSSRMPLTQQPR 565
            MNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSPCESLYCDNFRCCCFMCMCHKELPVTSSRMPLTQQPR
Sbjct:    1 MNDRCQCNAYRSFDTCLTNICPQRKTHSFADIVLCESWGCRACSESNLITVSPCESLYCDNFRCCCFMCMCHKELPVTSSRMPLTQQPR 89
The following BLAST results are available for this feature:
BLAST of SMED30021087 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0KHA44.844e-936.45Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
G4LWR86.906e-936.45Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A183NF971.931e-837.04Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A3P7YKZ61.970e-837.04Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A183JBL73.970e-836.11Uncharacterized protein OS=Schistosoma curassoni O... [more]
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BLAST of SMED30021087 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30021087 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000014860.13.281e-60100.00SMESG000014860.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021087 ID=SMED30021087|Name=SMED30021087|organism=Schmidtea mediterranea sexual|type=transcript|length=676bp
ATTGGACTATTTATTTAATGTTTCTATGTAATTATGTTTATCACCTAAAT
TAAATATGCCAGTGCAAATTGCTGATTTTAATGCTCCAAAATCTACAGAT
TTTCAAACACAAATTCAAAAAGATCTATCTAGCATTTCAATTCCTTTGTC
CTATTTGATATTCGTTATATTAATTGGTTATGTTGTTATAATAGCCATTA
TTTTTGGATCAATAAAACGAATTCTAAACAAAAGACCGTTATTTACTTGC
AGACCATTTGGCAGTGAAGATGAAATATGTTGCGGATGTTGTGCTTCAAT
GAACGATCGTTGCCAATGCAATGCATATCGGAGCTTTGATACCTGTCTTA
CTAATATCTGTCCTCAGAGAAAAACGCACAGCTTTGCTGATATAGTTCTT
TGCGAATCATGGGGATGTAGAGCATGTAGTGAATCAAATCTAATAACGGT
TTCCCCTTGTGAATCGCTTTATTGCGATAACTTCCGATGTTGTTGTTTTA
TGTGCATGTGTCACAAAGAGCTTCCGGTAACATCTAGTCGAATGCCTTTG
ACACAGCAGCCAAGATGAGATGATGCTCATTATGTGTTATTTAAGATGAT
ATCCACGAATTAAACTGACATCTGAATATGGCAATTATACAATTAATAAA
TGGCATTTCGTTTCTTCTACGAAAAA
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protein sequence of SMED30021087-orf-1

>SMED30021087-orf-1 ID=SMED30021087-orf-1|Name=SMED30021087-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=171bp
MPVQIADFNAPKSTDFQTQIQKDLSSISIPLSYLIFVILIGYVVIIAIIF
GSIKRILNKRPLFTCRPFGSEDEICCGCCASMNDRCQCNAYRSFDTCLTN
ICPQRKTHSFADIVLCESWGCRACSESNLITVSPCESLYCDNFRCCCFMC
MCHKELPVTSSRMPLTQQPR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 31..53