NPHS1-4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30021054 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of NPHS1-4 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 134.806 bits (338), Expect = 6.009e-31 Identity = 182/868 (20.97%), Postives = 324/868 (37.33%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKN--NYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIEL-------RIDQLKSNDK--IQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKEN-SREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAW--PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAE-----------PVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNSNFR--LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
F LP++ V+ +SV ++C V VQW ++LG D +PG R + GD ++GE +L I D EYECQV + L+ + +++ V P + L++TP +V + V+ C + + Q +S+ I++ N GSQ +L R+ S+++ + C + A +++ I+ S T+ +LF P P ++ L+ ++L + C V+ G+P+ ++ N P+S + ++ + + + + E H A L+C + + + V +PPS + I S+ S N +++L +C + + + W L ++ E+ +D + I + S + A +E N + L C A+ S+ + + Y K + G + + + L+C G P P L+WYK++ T++ + V +G S L DN C A ++ +S +A++ FP V I + L+ + L+C + SSNP ++ W +G RL P + K G + V D+ V C + + SS + RLNV++ P F + V A E + +V NP F W RG+R+ + L L ++N+TR G Y + N G E +DV YAP I ++ V ++ + C V +NP F W+
Sbjct: 34 FWALPEN-LTVVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKL--------------LLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISD-------ISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPA-------LEAPIKASFTVNVLF---PPGPPVIEWPGLDEGHVRAGQSLELPC-VARGGNPLATLQWLKNGQPVSTAWGTEH---------TQAVARSVLVMTVRPE-------------DHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSA------------------SQTENKNVTL--------SCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHI--------SMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLS------ASTQLAVQ--FPPTNVTILANASALR---PGDALNLTCVSVSSNPPVNLSW------DKEGERLEGVAAPPRRAPFK------------GSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFY----RLNVLYRPEFLGE--QVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDP-TEVNVGGSVDIVCTVDANPILPGMFNWE 776
BLAST of NPHS1-4 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 134.42 bits (337), Expect = 7.639e-31 Identity = 182/868 (20.97%), Postives = 324/868 (37.33%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKN--NYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIEL-------RIDQLKSNDK--IQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKEN-SREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAW--PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAE-----------PVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNSNFR--LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
F LP++ V+ +SV ++C V VQW ++LG D +PG R + GD ++GE +L I D EYECQV + L+ + +++ V P + L++TP +V + V+ C + + Q +S+ I++ N GSQ +L R+ S+++ + C + A +++ I+ S T+ +LF P P ++ L+ ++L + C V+ G+P+ ++ N P+S + ++ + + + + E H A L+C + + + V +PPS + I S+ S N +++L +C + + + W L ++ E+ +D + I + S + A +E N + L C A+ S+ + + Y K + G + + + L+C G P P L+WYK++ T++ + V +G S L DN C A ++ +S +A++ FP V I + L+ + L+C + SSNP ++ W +G RL P + K G + V D+ V C + + SS + RLNV++ P F + V A E + +V NP F W RG+R+ + L L ++N+TR G Y + N G E +DV YAP I ++ V ++ + C V +NP F W+
Sbjct: 34 FWALPEN-LTVVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKL--------------LLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISD-------ISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPA-------LEAPIKASFTVNVLF---PPGPPVIEWPGLDEGHVRAGQSLELPC-VARGGNPLATLQWLKNGQPVSTAWGTEH---------TQAVARSVLVMTVRPE-------------DHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSA------------------SQTENKNVTL--------SCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHI--------SMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLS------ASTQLAVQ--FPPTNVTILANASALR---PGDALNLTCVSVSSNPPVNLSW------DKEGERLEGVAAPPRRAPFK------------GSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFY----RLNVLYRPEFLGE--QVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDP-TEVNVGGSVDIVCTVDANPILPGMFNWE 776
BLAST of NPHS1-4 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 65.4698 bits (158), Expect = 3.472e-11 Identity = 34/97 (35.05%), Postives = 48/97 (49.48%), Query Frame = 2
Query: 431 VLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI 721
VL + C + G VQW + LG R +PG R I G+A+ G+H+L I ++ ED YECQ T+ L + AQ+ V V P P +
Sbjct: 35 VLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQ---ATQAGLRSRPAQLHVLVPPEAPQV 128
BLAST of NPHS1-4 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 65.855 bits (159), Expect = 6.357e-10 Identity = 34/97 (35.05%), Postives = 48/97 (49.48%), Query Frame = 2
Query: 431 VLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI 721
VL + C + G VQW + LG R +PG R I G+A+ G+H+L I ++ ED YECQ T+ L + AQ+ V V P P +
Sbjct: 35 VLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQ---ATQAGLRSRPAQLHVLVPPEAPQV 128
BLAST of NPHS1-4 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 65.855 bits (159), Expect = 6.582e-10 Identity = 34/97 (35.05%), Postives = 48/97 (49.48%), Query Frame = 2
Query: 431 VLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI 721
VL + C + G VQW + LG R +PG R I G+A+ G+H+L I ++ ED YECQ T+ L + AQ+ V V P P +
Sbjct: 35 VLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQ---ATQAGLRSRPAQLHVLVPPEAPQV 128
BLAST of NPHS1-4 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 113.62 bits (283), Expect = 7.536e-25 Identity = 206/968 (21.28%), Postives = 358/968 (36.98%), Query Frame = 2
Query: 1013 RKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTL------NINWSLAMKENSREQYNLDNIAQSEIIV--GLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENL--NLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQN--TLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEG--FHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISI 3874
+K I + T+ +LF P +P + L N + + + C V+ G+P P++ +W +N +L S + T+D+ E+ NI S+ + A C N +++ V YPP+ V+++ S+ R+ S N+ +L I W + N R ++ Q E +V G+ S + + E + +++ C A + + IKI P P ++G + E + +++ C + G PL +L WY+ + ++ A V+ G S L+ +V R N + +KC A N A I S +++ +P R V I+ +++L L C T SSNPA+ I W R + + L V L E R+ +G+ + L ++ + V C + P + ++ LNV++ P P V ++ NLT++ GNP I+ + W R + V ++ R+F L L G Y++ A N +G T I + V+Y+ I +I + + CE P S AN + W+ N + R K A L N+ + Q+ C N I A ++SAP V+++A C P W + ++ S+ N+ + + + I+ I P+ Y ++ C A N +GTDH + I+ + P+ P N+ T N + + W+ F+GGSDQ F +K RQ H V H N +SGL + + ++ S N S+ PVI TL++ +++
Sbjct: 213 QKETDIRITTNTTLDVLFP--PSDPTVEILR--NPSALRSGDNVTIACSVTG-GNPPPDV--FW--------YHENKRLQSHS-----------TLDTRSKEIK-NIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFGESN--------------------------------IRYGSSANIQCKSLPSNPASQITWII----NGR---SVPTPTQREFVVENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHV-IKIIAPPKAPIITGLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVA-GFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSV--YYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHL-------GDVSLNETTRD------NGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNT----SFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR----GNPAISLWQW-RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAA-TGDTVLLECEADGEP-SKANM-----------------IHWYKNGEILAAQHRG--------------------HKKAIL----RLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEK----DEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDY-------------TKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLV------NTTHTNL---RLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNV 1018 HSP 2 Score: 65.855 bits (159), Expect = 3.203e-10 Identity = 42/165 (25.45%), Postives = 78/165 (47.27%), Query Frame = 2
Query: 1772 LLCTAWPVDSVI-LSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQW 2263
+ C A+ ++ I ++ + + + + P+ PT+ A+ + +++ CS G P PD+ WY EN L + + + +I ++ SF S+ DN +C A N TGN ++ + +P V++ F I + + C++ SNPAS I W
Sbjct: 207 ITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKEIKNIYSFIASQNDNMAEYECRANNSRTGN--PKRKAMKLEVNYPPASVEL----FGESNIRYGSSANIQCKSLPSNPASQITW 365
BLAST of NPHS1-4 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 110.923 bits (276), Expect = 4.378e-24 Identity = 157/689 (22.79%), Postives = 263/689 (38.17%), Query Frame = 2
Query: 1826 IKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENL--NLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQN--TLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEG--FHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISI 3874
IKI P P ++G + E + +++ C + G PL +L WY+ + ++ A V+ G S L+ +V R N + +KC A N A I S +++ +P R V I+ +++L L C T SSNPA+ I W R + + L V L E R+ +G+ + L ++ + V C + P + ++ LNV++ P P V ++ NLT++ GNP I+ + W R + V ++ R+F L L G Y++ A N +G T I + V+Y+ I +I + + CE P S AN + W+ N + R K A L N+ + Q+ C N I A ++SAP V+++A C P W + ++ S+ N+ + + + I+ I P+ Y ++ C A N +GTDH + I+ + P+ P N+ T N + + W+ F+GGSDQ F +K RQ H V H N +SGL + + ++ S N S+ PVI TL++ +++
Sbjct: 123 IKIIAPPKAPIITGLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVA-GFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSV--YYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHL-------GDVSLNETTRD------NGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNT----SFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR----GNPAISLWQW-RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAA-TGDTVLLECEADGEP-SKANM-----------------IHWYKNGEILAAQHRG--------------------HKKAIL----RLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEK----DEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDY-------------TKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLV------NTTHTN---LRLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNV 716
BLAST of NPHS1-4 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 104.76 bits (260), Expect = 3.441e-22 Identity = 206/981 (21.00%), Postives = 359/981 (36.60%), Query Frame = 2
Query: 1013 RKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTL------NINWSLAMKENSREQYNLDNIAQSEIIV--GLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKEN-------------ATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENL--NLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQN--TLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEG--FHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISI 3874
+K I + T+ +LF P +P + L N + + + C V+ G+P P++ +W +N +L S + T+D+ E+ NI S+ + A C N +++ V YPP+ V+++ S+ R+ S N+ +L I W + N R ++ Q E +V G+ S + + E + +++ C A + + IKI P P ++G + E + +++ C + G PL +L WY+ + + ++ A V+ G S L+ +V R N + +KC A N A I S +++ +P R V I+ +++L L C T SSNPA+ I W R + + L V L E R+ +G+ + L ++ + V C + P + ++ LNV++ P P V ++ NLT++ GNP I+ + W R + V ++ R+F L L G Y++ A N +G T I + V+Y+ I +I + + CE P S AN + W+ N + R K A L N+ + Q+ C N I A ++SAP V+++A C P W + ++ S+ N+ + + + I+ I P+ Y ++ C A N +GTDH + I+ + P+ P N+ T N + + W+ F+GGSDQ F +K RQ H V H N +SGL + + ++ S N S+ PVI TL++ +++
Sbjct: 213 QKETDIRITTNTTLDVLFP--PSDPTVEILR--NPSALRSGDNVTIACSVTG-GNPPPDV--FW--------YHENKRLQSHS-----------TLDTRSKEIK-NIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFGESN--------------------------------IRYGSSANIQCKSLPSNPASQITWII----NGR---SVPTPTQREFVVENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHV-IKIIAPPKAPIITGLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVA-GFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSV--YYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHL-------GDVSLNETTRD------NGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNT----SFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR----GNPAISLWQW-RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVI-AATGDTVLLECEADGEP-SKANM-----------------IHWYKNGEILAAQHRG--------------------HKKAIL----RLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEK----DEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDY-------------TKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLV------NTTHTNL---RLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNV 1031 HSP 2 Score: 65.855 bits (159), Expect = 3.159e-10 Identity = 42/165 (25.45%), Postives = 78/165 (47.27%), Query Frame = 2
Query: 1772 LLCTAWPVDSVI-LSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQW 2263
+ C A+ ++ I ++ + + + + P+ PT+ A+ + +++ CS G P PD+ WY EN L + + + +I ++ SF S+ DN +C A N TGN ++ + +P V++ F I + + C++ SNPAS I W
Sbjct: 207 ITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKEIKNIYSFIASQNDNMAEYECRANNSRTGN--PKRKAMKLEVNYPPASVEL----FGESNIRYGSSANIQCKSLPSNPASQITW 365
BLAST of NPHS1-4 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 101.679 bits (252), Expect = 2.717e-21 Identity = 157/702 (22.36%), Postives = 264/702 (37.61%), Query Frame = 2
Query: 1826 IKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKEN-------------ATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENL--NLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQN--TLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEG--FHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISI 3874
IKI P P ++G + E + +++ C + G PL +L WY+ + + ++ A V+ G S L+ +V R N + +KC A N A I S +++ +P R V I+ +++L L C T SSNPA+ I W R + + L V L E R+ +G+ + L ++ + V C + P + ++ LNV++ P P V ++ NLT++ GNP I+ + W R + V ++ R+F L L G Y++ A N +G T I + V+Y+ I +I + + CE P S AN + W+ N + R K A L N+ + Q+ C N I A ++SAP V+++A C P W + ++ S+ N+ + + + I+ I P+ Y ++ C A N +GTDH + I+ + P+ P N+ T N + + W+ F+GGSDQ F +K RQ H V H N +SGL + + ++ S N S+ PVI TL++ +++
Sbjct: 123 IKIIAPPKAPIITGLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVA-GFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSV--YYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHL-------GDVSLNETTRD------NGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNT----SFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR----GNPAISLWQW-RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVI-AATGDTVLLECEADGEP-SKANM-----------------IHWYKNGEILAAQHRG--------------------HKKAIL----RLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEK----DEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDY-------------TKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLV------NTTHTN---LRLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNV 729
BLAST of NPHS1-4 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 90.1225 bits (222), Expect = 7.909e-18 Identity = 140/624 (22.44%), Postives = 232/624 (37.18%), Query Frame = 2
Query: 2021 SLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENL--NLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQN--TLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEG--FHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISI 3874
S L+ +V R N + +KC A N A I S +++ +P R V I+ +++L L C T SSNPA+ I W R + + L V L E R+ +G+ + L ++ + V C + P + ++ LNV++ P P V ++ NLT++ GNP I+ + W R + V ++ R+F L L G Y++ A N +G T I + V+Y+ I +I + + CE P S AN + W+ N + R K A L N+ + Q+ C N I A ++SAP V+++A C P W + ++ S+ N+ + + + I+ I P+ Y ++ C A N +GTDH + I+ + P+ P N+ T N + + W+ F+GGSDQ F +K RQ H V H N +SGL + + ++ S N S+ PVI TL++ +++
Sbjct: 20 STLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSV--YYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHL-------GDVSLNETTRD------NGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNT----SFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR----GNPAISLWQW-RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVI-AATGDTVLLECEADGEP-SKANM-----------------IHWYKNGEILAAQHRG--------------------HKKAILR----LNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEK----DEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDY-------------TKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLV------NTTHTNL---RLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNV 550
BLAST of NPHS1-4 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0088635) HSP 1 Score: 199.134 bits (505), Expect = 8.222e-51 Identity = 252/1185 (21.27%), Postives = 449/1185 (37.89%), Query Frame = 2
Query: 308 VNSFDLDDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVV---NPKLVLECFTNGLVTPLQFIWY-WNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQ--CRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVI---LSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDK-ISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLD--NENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHR---GNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCT---FVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNL 3808
+ L L + ++L S + Q+K F P D +VL + +++C V N G VQW LG+ +PG R +++GD +G +NL+ISN D +Y+CQV P +S +R A++TV P+ ++ I + N+ V N L L+C Q +WY N + E + V ++ + L++ +D + C+ +KA + + + T+ + PG P I S + +T+ ++CR S G+P ++ Y N + I + + +L + I +F E N R C ++ +N + V + V + P+ V + T A + G C+T I W + R+ N A S+ IV T + + A E N L+ ++ + + ++ I + Y P P +SGY + I LLC + G PL L WYK + +++ V+ DK +S+ ++ + DN +C A N AT S ++++ F VKI+ P +L+ +E T+I C++ SSNP + + WW DG + ++ ++ G + G T+S L++ V N C+S +++A ++ + L+V++ P F P S E +L + NP + W + + + + R+F + + N TR HR G YS A N G + + V+Y I +I+ P + + + C V P + + +W+ + Y ST F+N T + + D N R C N + + ++ + + APE + E C +G P W + Y ++ R + + + + + + ++ P Y A + C A N G VR+ + S+P+PP ++ L +T + + + W GF+GG S+R++ R + D N H+ + I GL N ++ V S+N L
Sbjct: 46 IKHLQLLRLLAVIVLASAPVPSHAQQQK---FRTTPHD-LQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPA--------------SIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKA-------LSPDMPMRATVQLSVLYPPGPPYIEGYSA--GETLRRGQTVELMCR-SRGGNPPAQLIWYKNGSQIRMAYRTSGRLSEN-------------IYTFTAEAGDNKARFR-----------CEASNVMSQNPLKAEVELSVLFAPTHVTVMG----------------------PTEARV----GDIVPLTCTTAPSNPPAEIKWMVG----GRQVRN----ATSKTIVSPEGGWTTTS-NITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINS---VIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVH--FAPETVKIRIEPEELRPGMEATII---CDSSSSNPPAKLSWW------KDG------------IPIEGINNTSKPGLWGG--TVSTLEFRVNVTQEMNGQVYTCQS----ANEALQRSAHEAVSLDVLYRPKFVP--PPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSL-NFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAM-LSCTVEGKPLTEEHVKWE-----------------RVGYDMTV----KTSTTFANGT---------------SYLHIKDAKREDVGNFR--CVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLI------VDKVAPADYGAYE--------------CVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY-RMADREQYKYIDGLPNSHKLT----IGGLRMNTLYLFSVMSWNEL 1044
BLAST of NPHS1-4 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0339277) HSP 1 Score: 198.749 bits (504), Expect = 1.031e-50 Identity = 252/1185 (21.27%), Postives = 449/1185 (37.89%), Query Frame = 2
Query: 308 VNSFDLDDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVV---NPKLVLECFTNGLVTPLQFIWY-WNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQ--CRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVI---LSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDK-ISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLD--NENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHR---GNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCT---FVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNL 3808
+ L L + ++L S + Q+K F P D +VL + +++C V N G VQW LG+ +PG R +++GD +G +NL+ISN D +Y+CQV P +S +R A++TV P+ ++ I + N+ V N L L+C Q +WY N + E + V ++ + L++ +D + C+ +KA + + + T+ + PG P I S + +T+ ++CR S G+P ++ Y N + I + + +L + I +F E N R C ++ +N + V + V + P+ V + T A + G C+T I W + R+ N A S+ IV T + + A E N L+ ++ + + ++ I + Y P P +SGY + I LLC + G PL L WYK + +++ V+ DK +S+ ++ + DN +C A N AT S ++++ F VKI+ P +L+ +E T+I C++ SSNP + + WW DG + ++ ++ G + G T+S L++ V N C+S +++A ++ + L+V++ P F P S E +L + NP + W + + + + R+F + + N TR HR G YS A N G + + V+Y I +I+ P + + + C V P + + +W+ + Y ST F+N T + + D N R C N + + ++ + + APE + E C +G P W + Y ++ R + + + + + + ++ P Y A + C A N G VR+ + S+P+PP ++ L +T + + + W GF+GG S+R++ R + D N H+ + I GL N ++ V S+N L
Sbjct: 46 IKHLQLLRLLAVIVLASAPVPSHAQQQK---FRTTPHD-LQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPA--------------SIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKA-------LSPDMPMRATVQLSVLYPPGPPYIEGYSA--GETLRRGQTVELMCR-SRGGNPPAQLIWYKNGSQIRMAYRTSGRLSEN-------------IYTFTAEAGDNKARFR-----------CEASNVMSQNPLKAEVELSVLFAPTHVTVMG----------------------PTEARV----GDIVPLTCTTAPSNPPAEIKWMVG----GRQVRN----ATSKTIVSPEGGWTTTS-NITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINS---VIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVH--FAPETVKIRIEPEELRPGMEATII---CDSSSSNPPAKLSWW------KDG------------IPIEGINNTSKPGLWGG--TVSTLEFRVNVTQEMNGQVYTCQS----ANEALQRSAHEAVSLDVLYRPKFVP--PPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSL-NFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAM-LSCTVEGKPLTEEHVKWE-----------------RVGYDMTV----KTSTTFANGT---------------SYLHIKDAKREDVGNFR--CVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLI------VDKVAPADYGAYE--------------CVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY-RMADREQYKYIDGLPNSHKLT----IGGLRMNTLYLFSVMSWNEL 1044
BLAST of NPHS1-4 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0111043) HSP 1 Score: 198.364 bits (503), Expect = 1.159e-50 Identity = 252/1185 (21.27%), Postives = 449/1185 (37.89%), Query Frame = 2
Query: 308 VNSFDLDDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVV---NPKLVLECFTNGLVTPLQFIWY-WNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQ--CRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVI---LSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDK-ISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLD--NENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHR---GNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCT---FVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNL 3808
+ L L + ++L S + Q+K F P D +VL + +++C V N G VQW LG+ +PG R +++GD +G +NL+ISN D +Y+CQV P +S +R A++TV P+ ++ I + N+ V N L L+C Q +WY N + E + V ++ + L++ +D + C+ +KA + + + T+ + PG P I S + +T+ ++CR S G+P ++ Y N + I + + +L + I +F E N R C ++ +N + V + V + P+ V + T A + G C+T I W + R+ N A S+ IV T + + A E N L+ ++ + + ++ I + Y P P +SGY + I LLC + G PL L WYK + +++ V+ DK +S+ ++ + DN +C A N AT S ++++ F VKI+ P +L+ +E T+I C++ SSNP + + WW DG + ++ ++ G + G T+S L++ V N C+S +++A ++ + L+V++ P F P S E +L + NP + W + + + + R+F + + N TR HR G YS A N G + + V+Y I +I+ P + + + C V P + + +W+ + Y ST F+N T + + D N R C N + + ++ + + APE + E C +G P W + Y ++ R + + + + + + ++ P Y A + C A N G VR+ + S+P+PP ++ L +T + + + W GF+GG S+R++ R + D N H+ + I GL N ++ V S+N L
Sbjct: 46 IKHLQLLRLLAVIVLASAPVPSHAQQQK---FRTTPHD-LQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPA--------------SIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKA-------LSPDMPMRATVQLSVLYPPGPPYIEGYSA--GETLRRGQTVELMCR-SRGGNPPAQLIWYKNGSQIRMAYRTSGRLSEN-------------IYTFTAEAGDNKARFR-----------CEASNVMSQNPLKAEVELSVLFAPTHVTVMG----------------------PTEARV----GDIVPLTCTTAPSNPPAEIKWMVG----GRQVRN----ATSKTIVSPEGGWTTTS-NITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINS---VIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVH--FAPETVKIRIEPEELRPGMEATII---CDSSSSNPPAKLSWW------KDG------------IPIEGINNTSKPGLWGG--TVSTLEFRVNVTQEMNGQVYTCQS----ANEALQRSAHEAVSLDVLYRPKFVP--PPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSL-NFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAM-LSCTVEGKPLTEEHVKWE-----------------RVGYDMTV----KTSTTFANGT---------------SYLHIKDAKREDVGNFR--CVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLI------VDKVAPADYGAYE--------------CVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY-RMADREQYKYIDGLPNSHKLT----IGGLRMNTLYLFSVMSWNEL 1044
BLAST of NPHS1-4 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087413) HSP 1 Score: 172.94 bits (437), Expect = 5.848e-43 Identity = 194/820 (23.66%), Postives = 322/820 (39.27%), Query Frame = 2
Query: 392 LQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI--YFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSND--KIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVD-SVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFV--ECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESN--SNFRLFQNTLR--IFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVG 2818
+Q F P D ++L + +++C V N+ GKVQW LG+ +PG R +++ DA + +NLQI N ED EY+CQV P + +R A++++ PS VI Y R ++ + N L L C + +W+ VS V+ T+ + L + +D + C +KA M++ ++LSV Y PG P S + + + + CR S G+P P L ++ N V I S + T LS N+ + + ANL C K LL + + + V Y P D Y+S K S+ L +C T I+WS+ + Y + + + ++S + L + +C A + + + S + + Y P+ P L+GY + + + L CS+ G P P L WYK + ++ +V + KI+S LS V+ DN I KC +N A ++ ++ + FP VKI P K ++ L C++ SSNP + I WW K IP + L G + G ++S L+ V + + + C+S +++ S+H + + L++++ P F + P + F E ++ GNP + + W + + SN S RL + R I L+R G Y A N G + + V+YAP I
Sbjct: 38 VQKFHLTPHD-LQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARNARVEVE-------ERQN------LTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKA-LPPDVPMRAQVQLSV-----LYP-PGAPFFEGYS--QGETLHRGQEVQIACR-SRGGNP-----------PAQLTWYR---------NGVAISSPQRT----SGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPK------------------DVYLSGANQAKVGDSVQL--------SCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWV-------SSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDS--KITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVH--FPPETVKISVVP---KNLVPGIRAKLICDSSSSNPPAKISWW-------------KDGIPVEGLNLANR-----PGLWGG--SVSTLEMYVNITQDLDGIVYTCQS---HNEVLQRSVH-ETISLDILYPPKF--ETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITA 743
BLAST of NPHS1-4 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087414) HSP 1 Score: 172.555 bits (436), Expect = 7.896e-43 Identity = 194/820 (23.66%), Postives = 322/820 (39.27%), Query Frame = 2
Query: 392 LQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI--YFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSND--KIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVD-SVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFV--ECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESN--SNFRLFQNTLR--IFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVG 2818
+Q F P D ++L + +++C V N+ GKVQW LG+ +PG R +++ DA + +NLQI N ED EY+CQV P + +R A++++ PS VI Y R ++ + N L L C + +W+ VS V+ T+ + L + +D + C +KA M++ ++LSV Y PG P S + + + + CR S G+P P L ++ N V I S + T LS N+ + + ANL C K LL + + + V Y P D Y+S K S+ L +C T I+WS+ + Y + + + ++S + L + +C A + + + S + + Y P+ P L+GY + + + L CS+ G P P L WYK + ++ +V + KI+S LS V+ DN I KC +N A ++ ++ + FP VKI P K ++ L C++ SSNP + I WW K IP + L G + G ++S L+ V + + + C+S +++ S+H + + L++++ P F + P + F E ++ GNP + + W + + SN S RL + R I L+R G Y A N G + + V+YAP I
Sbjct: 38 VQKFHLTPHD-LQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARNARVEVE-------ERQN------LTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKA-LPPDVPMRAQVQLSV-----LYP-PGAPFFEGYS--QGETLHRGQEVQIACR-SRGGNP-----------PAQLTWYR---------NGVAISSPQRT----SGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPK------------------DVYLSGANQAKVGDSVQL--------SCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWV-------SSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDS--KITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVH--FPPETVKISVVP---KNLVPGIRAKLICDSSSSNPPAKISWW-------------KDGIPVEGLNLANR-----PGLWGG--SVSTLEMYVNITQDLDGIVYTCQS---HNEVLQRSVH-ETISLDILYPPKF--ETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITA 743
BLAST of NPHS1-4 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 164.851 bits (416), Expect = 2.684e-40 Identity = 191/847 (22.55%), Postives = 348/847 (41.09%), Query Frame = 2
Query: 395 QCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITS--LSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSA-SIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWL-RGHRVL-ESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
Q F+ P++ V ++ ++KC V G VQW ++LG RS+PG R ++ GD KG+++LQI ++ ED G YECQV + + ++ + + V + S P YF+ + ++ +V + + C P + + + + + E+ +S + L N +++ +R ++ CR +N AS L +T+ Y P P I+ L N +V + L +VC +S G+P+ ++W + NG E+ S +D F + +++ + + + A L C + ++ T + V + P+ V + S FE++ +G+ +CST+ ++I W L KE +R + ++ T S + K ++E N + L C A+ + S + + +YY P K L ++ T+ L+C + G P L W K N + A VS+ +S L + DN +C + N A + S R +FPA VKI + + K++ + L C SSNP ++I W + GP V LK + + E+ G SKL +++ + C++ + ++ + L+V+F P FS + P+ Q E+ T+ V NP W+ +G +++ E + + TL I N++R G+Y + N G +DV+Y+P V ++ + V + C +NP +++ F WK
Sbjct: 72 QAFKTEPRN-VTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQP--YFQLE------------SEEPWVEGEQYTVTCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPRA---------LETGMTMKVYFPPQAPMIMGLE--NEEV-KAGSFLKVVC-MSYGGNPL--ATLHW----------------TKNG---EVLSTSWDVD-FVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLLGS-----------------FEAV---------EGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMM-------TVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAK---KVLSTQTKFRVLFPAVSVKIVA---KQKELRRGQTLDLDCLAGSSNPTANISWSL-------GP-----------VILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEGVNNFY----SLDVLFPPEFSDEQPD--EVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPT-VRMKSDPVFVNIGDTADLLCVADANPITSSMFSWK 804
BLAST of NPHS1-4 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 163.696 bits (413), Expect = 5.218e-40 Identity = 191/847 (22.55%), Postives = 348/847 (41.09%), Query Frame = 2
Query: 395 QCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITS--LSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSA-SIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWL-RGHRVL-ESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
Q F+ P++ V ++ ++KC V G VQW ++LG RS+PG R ++ GD KG+++LQI ++ ED G YECQV + + ++ + + V + S P YF+ + ++ +V + + C P + + + + + E+ +S + L N +++ +R ++ CR +N AS L +T+ Y P P I+ L N +V + L +VC +S G+P+ ++W + NG E+ S +D F + +++ + + + A L C + ++ T + V + P+ V + S FE++ +G+ +CST+ ++I W L KE +R + ++ T S + K ++E N + L C A+ + S + + +YY P K L ++ T+ L+C + G P L W K N + A VS+ +S L + DN +C + N A + S R +FPA VKI + + K++ + L C SSNP ++I W + GP V LK + + E+ G SKL +++ + C++ + ++ + L+V+F P FS + P+ Q E+ T+ V NP W+ +G +++ E + + TL I N++R G+Y + N G +DV+Y+P V ++ + V + C +NP +++ F WK
Sbjct: 27 QAFKTEPRN-VTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQP--YFQLE------------SEEPWVEGEQYTVTCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPRA---------LETGMTMKVYFPPQAPMIMGLE--NEEV-KAGSFLKVVC-MSYGGNPL--ATLHW----------------TKNG---EVLSTSWDVD-FVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLLGS-----------------FEAV---------EGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMM-------TVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAK---KVLSTQTKFRVLFPAVSVKIVA---KQKELRRGQTLDLDCLAGSSNPTANISWSL-------GP-----------VILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEGVNNFY----SLDVLFPPEFSDEQPD--EVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPT-VRMKSDPVFVNIGDTADLLCVADANPITSSMFSWK 759
BLAST of NPHS1-4 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 109.383 bits (272), Expect = 2.130e-23 Identity = 109/460 (23.70%), Postives = 191/460 (41.52%), Query Frame = 2
Query: 1547 ASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSA-SIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWL-RGHRVL-ESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
S ++G+ +CST+ ++I W L KE +R + ++ T S + K ++E N + L C A+ + S + + +YY P K L ++ T+ L+C + G P L W K N + A VS+ +S L + DN +C + N A + S R +FPA VKI + + K++ + L C SSNP ++I W + GP V LK + + E+ G SKL +++ + C++ + ++ + L+V+F P FS + P+ Q E+ T+ V NP W+ +G +++ E + + TL I N++R G+Y + N G +DV+Y+P V ++ + V + C +NP +++ F WK
Sbjct: 211 GSFEAVEGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMM-------TVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAK---KVLSTQTKFRVLFPAVSVKIVA---KQKELRRGQTLDLDCLAGSSNPTANISWSL-------GP-----------VILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEGVNNFY----SLDVLFPPEFSDEQPD--EVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPT-VRMKSDPVFVNIGDTADLLCVADANPITSSMFSWK 632 HSP 2 Score: 79.337 bits (194), Expect = 3.379e-14 Identity = 78/323 (24.15%), Postives = 132/323 (40.87%), Query Frame = 2
Query: 1712 FKQTNSFVELKATKEINNMDLLCTAW-PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVS---GEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPH---FSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVL 2659
+ T++ V ++A + L C A P L + +K+Y+ P P + G E + + ++C + G PL L W K LS + V + SS+L +V EDN +++C + N ++ + +++ + + T + +E I LSC T SSNP I+WW + KEL+R V+ G+ G T+S L + V DN ++CE+ +K T L V + P PN VF + + F+ GNP + WL+ ++V+
Sbjct: 61 LQNTHAEVTIRARSSDDTRRLTCRAKNPASPRALETGMTMKVYFPPQAPMIMGLE-NEEVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSIN------QVTRSPMSLTRTLTVLFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVHIRWW---------------------LGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEA----FNKGTRFSSVRAAELIVYYPPQKVWLDAPPPN--VFLHSGSTLRLVCFSSGGNP-TGQLTWLKNNKVV 348
BLAST of NPHS1-4 vs. Ensembl Zebrafish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 88.9669 bits (219), Expect = 2.803e-17 Identity = 84/303 (27.72%), Postives = 128/303 (42.24%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQ-KKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKLFQNFKLDSSNGNSVEIKS 1285
F PQD+ V+ SV + CV+ G VQW + LG +R +PG R +++GD GEH+L I + + D YECQ T+ L +A++TV V PS P++ +L H TP N L C +G + WY + +++ Q + SL +G S + + + S CRV N A+ R + SVTI + P + LSV V E + L +C S PEI Y W+ + +NG+S+E+ +
Sbjct: 20 FSQQPQDQV-VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWVRYSLMGDPLSGEHSLVIDSAELGDDAIYECQ---ATQAGLRSHRAKLTVLVPPSDPIVEGGPVVRLKAH----TPHN-----------LTCRASGAKPAAEITWYRDGEIM----QNAIYSKSLMEDGKRETAVSMLPMVPEDSDSGRTYTCRVLNPAAPAGR-------QTSVTINVQ------HPPSVTLSVQPQTVMEGAKVL-FICSASAN----PEITGYRWSKG--------GVPISEANGDSLEVTA 273
BLAST of NPHS1-4 vs. Ensembl Zebrafish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 88.9669 bits (219), Expect = 2.910e-17 Identity = 84/303 (27.72%), Postives = 128/303 (42.24%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQ-KKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKLFQNFKLDSSNGNSVEIKS 1285
F PQD+ V+ SV + CV+ G VQW + LG +R +PG R +++GD GEH+L I + + D YECQ T+ L +A++TV V PS P++ +L H TP N L C +G + WY + +++ Q + SL +G S + + + S CRV N A+ R + SVTI + P + LSV V E + L +C S PEI Y W+ + +NG+S+E+ +
Sbjct: 16 FSQQPQDQV-VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWVRYSLMGDPLSGEHSLVIDSAELGDDAIYECQ---ATQAGLRSHRAKLTVLVPPSDPIVEGGPVVRLKAH----TPHN-----------LTCRASGAKPAAEITWYRDGEIM----QNAIYSKSLMEDGKRETAVSMLPMVPEDSDSGRTYTCRVLNPAAPAGR-------QTSVTINVQ------HPPSVTLSVQPQTVMEGAKVL-FICSASAN----PEITGYRWSKG--------GVPISEANGDSLEVTA 269
BLAST of NPHS1-4 vs. Ensembl Xenopus
Match: NPHS1 (NPHS1 nephrin [Source:NCBI gene;Acc:100494620]) HSP 1 Score: 170.244 bits (430), Expect = 6.369e-42 Identity = 206/864 (23.84%), Postives = 345/864 (39.93%), Query Frame = 2
Query: 380 IQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVI--YFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELR--------IDQLKSNDKI-QCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVIL---SASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNS--NFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
+ K Q F P D V+ S+ ++ C V + G VQW ++LG DR++PG R ++ GD KGE+NL+I + +D EY CQV + ++ + A + V + P P Y ++T + + P L +GL EVS S SN GS+ +L I Q N K+ C N A +++ I +S T+ +LF P P I + K ETL ++C +S G+P+ ++ W+ N ++ S R D S + + L + + A ++C + +++ + V + P+ V I SS + + F+C T + I W L KE + + K T S + ++A +E + M L C A+ + IL S S+ + + Y P + + ++ + C + G P P L W K+ +LS + S G ++ ++ DN +C A NI G + S ++ FPA V I S+ K+ + I L+C T SSNPAS I W DG +L K ++L R+ Y G + S++ DN V CE+ ++A ++ + +LN++F P F P +V QA E+ I V NP + W L G +++ + + + L I+N++R G Y++ N GK I+ +DV+Y+P I ++ V + + C +NP + + FQW+
Sbjct: 14 VTAKQQVFRVQP-DNITVVQGSTALLSCEVEHGTGMVQWEKNGLLLGPDRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGRSEVSLGIISRTALINVLIPPKAPTFEEYAAGSRVTWVYGVEYTVTCQATDAKPAAALTIKKSGL---------------------EVSGDSSSNPGSRDKLENTAMVAKVIPQSSDNGKLLTCLALNPA-------LQTPITVSFTMNVLFP--PNAPIIEGYIGPSVKA---GETLKLIC-ISLSGNPLATLQ--WSKND-------------------DVVSTRWETDEVV-RASRSHLTLNIKPADNMAVVSCEAVNHVTPETLKTSIILNVVFLPTEVTILGSSSGPEKSNLT--------------------------FSCFTAASNPPVQIRWWLGPKELVNTIVTISDAVHGG-------KVTMSNLTMEALREEHGMALTCEAF--NEAILYTKSNSVTLSVQYPPQNVWIEAPPADKFFRAGTSVKMTCFSSGGNPPPRLTWIKDTKSLSGGSQIHS-GKIVTKEITIITVPSDNMATYRCNASNI--GKTPALTASTKLKVQFPAIDVNIISS---AKEYRRGSTIILTCVTGSSNPASTISW------VKDGEQL----------KAQDLGRK--PSLYGGISSSSRVTLKAASKDNGRRVSCEAFSSVLNEAVNTFY----KLNILFPPEFLDDQP--AVVQAVEHGAALIPVRVRANPPQINYTWSLWGEKLIRDGAYRHHLRGEGALEIWNVSRADSGVYNVTCVNKEGKNSIVIRLDVQYSPTIRSLGDT--EVDLGSDAEIACTADANPATDSMFQWR 753 HSP 2 Score: 57.7658 bits (138), Expect = 1.822e-7 Identity = 20/57 (35.09%), Postives = 37/57 (64.91%), Query Frame = 2
Query: 3497 CQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIK 3667
C A N G D +++V S+P+PP ++ + T N++ ++W GF+GG++Q FR++
Sbjct: 867 CTATNTLGVDSFTIQLVSPSRPDPPSGLRVIGFTHNWVTLQWSAGFDGGAEQKFRVR 923
BLAST of NPHS1-4 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 85.1149 bits (209), Expect = 5.376e-16 Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQ--LKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKL 1231
F P D+ ++ SV + CVV G VQW + LG +R +PG R +++GD S GEHNL I ++ D YECQ T+ +L ++A++TV + P P+I + L+ P N L C + WY + + + + ++ + GS L I S CRV N+A R++ SVT+++ + P + LSV + E + VS PE+ Y W + L +
Sbjct: 30 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQ---ATQAALRSQRARLTVLLPPGDPMI---PGSPVANVLLNVPYN-----------LTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPEGREK-------SVTLSVQY------PPKVTLSVEPPTITEGGSVTFLCGAVS-----NPEVTGYRWAKGGVPLAV 273
BLAST of NPHS1-4 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 84.7297 bits (208), Expect = 6.445e-16 Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQ--LKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKL 1231
F P D+ ++ SV + CVV G VQW + LG +R +PG R +++GD S GEHNL I ++ D YECQ T+ +L ++A++TV + P P+I + L+ P N L C + WY + + + + ++ + GS L I S CRV N+A R++ SVT+++ + P + LSV + E + VS PE+ Y W + L +
Sbjct: 30 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQ---ATQAALRSQRARLTVLLPPGDPMI---PGSPVANVLLNVPYN-----------LTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPEGREK-------SVTLSVQY------PPKVTLSVEPPTITEGGSVTFLCGAVSN-----PEVTGYRWAKGGVPLAV 273
BLAST of NPHS1-4 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 84.3445 bits (207), Expect = 8.207e-16 Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQ--LKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKL 1231
F P D+ ++ SV + CVV G VQW + LG +R +PG R +++GD S GEHNL I ++ D YECQ T+ +L ++A++TV + P P+I + L+ P N L C + WY + + + + ++ + GS L I S CRV N+A R++ SVT+++ + P + LSV + E + VS PE+ Y W + L +
Sbjct: 38 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQ---ATQAALRSQRARLTVLLPPGDPMI---PGSPVANVLLNVPYN-----------LTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPEGREK-------SVTLSVQY------PPKVTLSVEPPTITEGGSVTFLCGAVS-----NPEVTGYRWAKGGVPLAV 281
BLAST of NPHS1-4 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 83.9593 bits (206), Expect = 1.152e-15 Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQ--LKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKL 1231
F P D+ ++ SV + CVV G VQW + LG +R +PG R +++GD S GEHNL I ++ D YECQ T+ +L ++A++TV + P P+I + L+ P N L C + WY + + + + ++ + GS L I S CRV N+A R++ SVT+++ + P + LSV + E + VS PE+ Y W + L +
Sbjct: 17 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQ---ATQAALRSQRARLTVLLPPGDPMI---PGSPVANVLLNVPYN-----------LTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPEGREK-------SVTLSVQY------PPKVTLSVEPPTITEGGSVTFLCGAVS-----NPEVTGYRWAKGGVPLAV 260
BLAST of NPHS1-4 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 122.865 bits (307), Expect = 2.195e-27 Identity = 188/858 (21.91%), Postives = 321/858 (37.41%), Query Frame = 2
Query: 431 VLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNN--YVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQ-------IELRIDQLKSNDK--IQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTN-SFVELKATKEINNMDLLCTAW--PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNA----EP-------VVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNSNFR--LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
V+ S+V + C VR VQW ++LG + +PG R ++ GD++KGE +L I D EYECQV S L + V PSV + K+ L +TP + +V + V+ C + I+ + V + ++S N GS+ E R+ S++ + C N A + + I+ S T+ ILF P P +++ LN E L + C ++ G+P ++ N P+S+ + + + + + + E H A L+C + ++ + V +PPS V I S+ S N +++L C T + + W L R+ D ++ GL+ + S + L +E N + L C A+ S+ + + Y K + G ++I + L+C G P P L W K++ +++ EP V +G S L + DN C A + S S + FP + I + L+ + L+C + SSNP ++ LD +L ++ + S + G + V D+ + V C + + SS + RLNV++ P F +Q +V + + L + +V NP F W RG+R+ + L L+++N+TR G Y + N G E L +DV YAP I ++ V ++ + C V +NP F W+
Sbjct: 43 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQV----GRSELGPEL-----VSPSVILSILVSPKV----LQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVED-------VSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPA-------LATPIKASFTMNILF---PPGPPVIDWPGLNEGHVRAGENLELPC-IARGGNPPATLQWLKNGKPVSIAWGTEH---------AQAVAHSVLVMTVRPE-------------DHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGST------------------SQSENKNVTL--------CCLTKSSRPRVLLRWWLG----GRQLLPTDET----VMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQL--------SASTQLVVQFPPTNLTILANSSALR---PGDALNLTCVSISSNPPVNLS--------LDK----------EGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFY----RLNVLYPPEFLGEQVRAVTVVEQGQAL---LPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDP-TEVNVGGSVDIVCTVDANPILPEMFSWE 776
BLAST of NPHS1-4 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 122.479 bits (306), Expect = 2.784e-27 Identity = 188/858 (21.91%), Postives = 321/858 (37.41%), Query Frame = 2
Query: 431 VLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNN--YVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQ-------IELRIDQLKSNDK--IQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTN-SFVELKATKEINNMDLLCTAW--PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNA----EP-------VVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNSNFR--LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
V+ S+V + C VR VQW ++LG + +PG R ++ GD++KGE +L I D EYECQV S L + V PSV + K+ L +TP + +V + V+ C + I+ + V + ++S N GS+ E R+ S++ + C N A + + I+ S T+ ILF P P +++ LN E L + C ++ G+P ++ N P+S+ + + + + + + E H A L+C + ++ + V +PPS V I S+ S N +++L C T + + W L R+ D ++ GL+ + S + L +E N + L C A+ S+ + + Y K + G ++I + L+C G P P L W K++ +++ EP V +G S L + DN C A + S S + FP + I + L+ + L+C + SSNP ++ LD +L ++ + S + G + V D+ + V C + + SS + RLNV++ P F +Q +V + + L + +V NP F W RG+R+ + L L+++N+TR G Y + N G E L +DV YAP I ++ V ++ + C V +NP F W+
Sbjct: 57 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQV----GRSELGPEL-----VSPSVILSILVSPKV----LQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVED-------VSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPA-------LATPIKASFTMNILF---PPGPPVIDWPGLNEGHVRAGENLELPC-IARGGNPPATLQWLKNGKPVSIAWGTEH---------AQAVAHSVLVMTVRPE-------------DHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGST------------------SQSENKNVTL--------CCLTKSSRPRVLLRWWLG----GRQLLPTDET----VMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQL--------SASTQLVVQFPPTNLTILANSSALR---PGDALNLTCVSISSNPPVNLS--------LDK----------EGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFY----RLNVLYPPEFLGEQVRAVTVVEQGQAL---LPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDP-TEVNVGGSVDIVCTVDANPILPEMFSWE 790
BLAST of NPHS1-4 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 66.2402 bits (160), Expect = 3.026e-10 Identity = 32/102 (31.37%), Postives = 54/102 (52.94%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLP 706
F P D+ V+ V+ CV+ N G VQW + LG + + R ++G A G++NL+I++ + D YECQ T+ +L ++A++TV + P
Sbjct: 55 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQ---ATEAALRSRRAKLTVLIPP 152
BLAST of NPHS1-4 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 65.4698 bits (158), Expect = 6.088e-10 Identity = 32/102 (31.37%), Postives = 54/102 (52.94%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLP 706
F P D+ V+ V+ CV+ N G VQW + LG + + R ++G A G++NL+I++ + D YECQ T+ +L ++A++TV + P
Sbjct: 55 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQ---ATEAALRSRRAKLTVLIPP 152
BLAST of NPHS1-4 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 65.4698 bits (158), Expect = 6.088e-10 Identity = 32/102 (31.37%), Postives = 54/102 (52.94%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLP 706
F P D+ V+ V+ CV+ N G VQW + LG + + R ++G A G++NL+I++ + D YECQ T+ +L ++A++TV + P
Sbjct: 55 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQ---ATEAALRSRRAKLTVLIPP 152
BLAST of NPHS1-4 vs. UniProt/SwissProt
Match: sp|O60500|NPHN_HUMAN (Nephrin OS=Homo sapiens OX=9606 GN=NPHS1 PE=1 SV=1) HSP 1 Score: 134.806 bits (338), Expect = 2.886e-30 Identity = 182/868 (20.97%), Postives = 324/868 (37.33%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKN--NYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIEL-------RIDQLKSNDK--IQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKEN-SREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAW--PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAE-----------PVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNSNFR--LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
F LP++ V+ +SV ++C V VQW ++LG D +PG R + GD ++GE +L I D EYECQV + L+ + +++ V P + L++TP +V + V+ C + + Q +S+ I++ N GSQ +L R+ S+++ + C + A +++ I+ S T+ +LF P P ++ L+ ++L + C V+ G+P+ ++ N P+S + ++ + + + + E H A L+C + + + V +PPS + I S+ S N +++L +C + + + W L ++ E+ +D + I + S + A +E N + L C A+ S+ + + Y K + G + + + L+C G P P L+WYK++ T++ + V +G S L DN C A ++ +S +A++ FP V I + L+ + L+C + SSNP ++ W +G RL P + K G + V D+ V C + + SS + RLNV++ P F + V A E + +V NP F W RG+R+ + L L ++N+TR G Y + N G E +DV YAP I ++ V ++ + C V +NP F W+
Sbjct: 34 FWALPEN-LTVVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKL--------------LLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISD-------ISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPA-------LEAPIKASFTVNVLF---PPGPPVIEWPGLDEGHVRAGQSLELPC-VARGGNPLATLQWLKNGQPVSTAWGTEH---------TQAVARSVLVMTVRPE-------------DHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSA------------------SQTENKNVTL--------SCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHI--------SMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLS------ASTQLAVQ--FPPTNVTILANASALR---PGDALNLTCVSVSSNPPVNLSW------DKEGERLEGVAAPPRRAPFK------------GSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFY----RLNVLYRPEFLGE--QVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDP-TEVNVGGSVDIVCTVDANPILPGMFNWE 776
BLAST of NPHS1-4 vs. UniProt/SwissProt
Match: sp|Q9R044|NPHN_RAT (Nephrin OS=Rattus norvegicus OX=10116 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 124.02 bits (310), Expect = 5.777e-27 Identity = 186/859 (21.65%), Postives = 324/859 (37.72%), Query Frame = 2
Query: 431 VLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNN--YVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQL------KSNDKIQ---CRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTN-SFVELKATKEINNMDLLCTAW--PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNA----EP-------VVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNSNFR--LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
+ ++V + C VR VQW ++LG + +PG R ++ GD +KGE +L I D EYECQV + L+ K +++ V P V L++TP + +V + V+ C + G P I + ++ + + ++S N GS+ +L I + +S+D Q C N A + + I+ S T+ ILF P P +++ LN E L + C G+P P +L+ +N K S+ + ++ ++ +++ + + H A L+C + ++ + V +PPS + I S+S + + C T + + W L R+ D ++ GL+ + S + +E N + L C A+ S+ + + Y K + G + I + L+C G P P L+W+K++ +S EP V +G S L + DN C A + S S + FP + I + L+ + L+C + SSNP ++ W +G RL +++ + S + G + V D+ V C + + SS + R NV++ P F +Q +V + + L + +V NP F W RG+R+ + L L+++N+TR G Y + N G E L +DV YAP I R+ V ++ + C V +NP F W+
Sbjct: 57 AVEGTTVKLWCGVRAPGSVVQWAKDGLLLGPNPKMPGFPRYSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSPKV--------------LLLTPEAGSTVTWVAGQEYVVTCVS-GDAKPAPDITF------IQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPA-------LDTPIKASFTMNILF---PPGPPVIDWPGLNEGHVRAGENLELPCTAR-GGNP-----------PATLQWLKNGKPVSTAWGTEHAQA-----------VAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITIL--------------------------GSVSQSENKNVTLCCLTKSSRPRVLLRWWLG----GRQLLPTDET----VMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAGQL--------SASTQLVVQFPPTNLTILANSSALR---PGDALNLTCVSISSNPPVNLSW------DKEGERL------------EDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFY----RFNVLYPPEFLGEQVRAVTVVEQGQVL---LPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDP-TEVNVGGSVDIVCTVDANPILPEMFSWE 790
BLAST of NPHS1-4 vs. UniProt/SwissProt
Match: sp|Q9QZS7|NPHN_MOUSE (Nephrin OS=Mus musculus OX=10090 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 122.479 bits (306), Expect = 1.947e-26 Identity = 188/858 (21.91%), Postives = 321/858 (37.41%), Query Frame = 2
Query: 431 VLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNN--YVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQ-------IELRIDQLKSNDK--IQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTN-SFVELKATKEINNMDLLCTAW--PVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNA----EP-------VVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVW-LRGHRVLESNSNFR--LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
V+ S+V + C VR VQW ++LG + +PG R ++ GD++KGE +L I D EYECQV S L + V PSV + K+ L +TP + +V + V+ C + I+ + V + ++S N GS+ E R+ S++ + C N A + + I+ S T+ ILF P P +++ LN E L + C ++ G+P ++ N P+S+ + + + + + + E H A L+C + ++ + V +PPS V I S+ S N +++L C T + + W L R+ D ++ GL+ + S + L +E N + L C A+ S+ + + Y K + G ++I + L+C G P P L W K++ +++ EP V +G S L + DN C A + S S + FP + I + L+ + L+C + SSNP ++ LD +L ++ + S + G + V D+ + V C + + SS + RLNV++ P F +Q +V + + L + +V NP F W RG+R+ + L L+++N+TR G Y + N G E L +DV YAP I ++ V ++ + C V +NP F W+
Sbjct: 57 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQV----GRSELGPEL-----VSPSVILSILVSPKV----LQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVED-------VSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPA-------LATPIKASFTMNILF---PPGPPVIDWPGLNEGHVRAGENLELPC-IARGGNPPATLQWLKNGKPVSIAWGTEH---------AQAVAHSVLVMTVRPE-------------DHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGST------------------SQSENKNVTL--------CCLTKSSRPRVLLRWWLG----GRQLLPTDET----VMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQL--------SASTQLVVQFPPTNLTILANSSALR---PGDALNLTCVSISSNPPVNLS--------LDK----------EGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFY----RLNVLYPPEFLGEQVRAVTVVEQGQAL---LPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDP-TEVNVGGSVDIVCTVDANPILPEMFSWE 790
BLAST of NPHS1-4 vs. UniProt/SwissProt
Match: sp|Q9U3P2|SYG2_CAEEL (Synaptogenesis protein syg-2 OS=Caenorhabditis elegans OX=6239 GN=syg-2 PE=1 SV=1) HSP 1 Score: 90.5077 bits (223), Expect = 1.088e-16 Identity = 140/624 (22.44%), Postives = 232/624 (37.18%), Query Frame = 2
Query: 2021 SLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENL--NLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQN--TLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEG--FHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISI 3874
S L+ +V R N + +KC A N A I S +++ +P R V I+ +++L L C T SSNPA+ I W R + + L V L E R+ +G+ + L ++ + V C + P + ++ LNV++ P P V ++ NLT++ GNP I+ + W R + V ++ R+F L L G Y++ A N +G T I + V+Y+ I +I + + CE P S AN + W+ N + R K A L N+ + Q+ C N I A ++SAP V+++A C P W + ++ S+ N+ + + + I+ I P+ Y ++ C A N +GTDH + I+ + P+ P N+ T N + + W+ F+GGSDQ F +K RQ H V H N +SGL + + ++ S N S+ PVI TL++ +++
Sbjct: 541 STLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSV--YYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHL-------GDVSLNETTRD------NGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNT----SFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR----GNPAISLWQW-RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVI-AATGDTVLLECEADGEP-SKANM-----------------IHWYKNGEILAAQHRG--------------------HKKAILR----LNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEK----DEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDY-------------TKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLV------NTTHTNL---RLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNV 1071 HSP 2 Score: 65.855 bits (159), Expect = 3.339e-9 Identity = 42/165 (25.45%), Postives = 78/165 (47.27%), Query Frame = 2
Query: 1772 LLCTAWPVDSVI-LSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQW 2263
+ C A+ ++ I ++ + + + + P+ PT+ A+ + +++ CS G P PD+ WY EN L + + + +I ++ SF S+ DN +C A N TGN ++ + +P V++ F I + + C++ SNPAS I W
Sbjct: 207 ITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKEIKNIYSFIASQNDNMAEYECRANNSRTGN--PKRKAMKLEVNYPPASVEL----FGESNIRYGSSANIQCKSLPSNPASQITW 365
BLAST of NPHS1-4 vs. UniProt/SwissProt
Match: sp|B1Q236|SYG1_CAEEL (Synaptogenesis protein syg-1 OS=Caenorhabditis elegans OX=6239 GN=syg-1 PE=1 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 3.013e-10 Identity = 36/108 (33.33%), Postives = 62/108 (57.41%), Query Frame = 2
Query: 407 DLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRS--VPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVI 721
+ P+D + + ++ C V +Q G VQW + LG DR +PG +R ++G A+ GE+NL+ISN+ D ++ CQ+ + +++ KA++TV V P+ P I
Sbjct: 27 EAPKDTLAAV-GETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKI 133
BLAST of NPHS1-4 vs. TrEMBL
Match: A0A0H3YFF3 (NPHS1-4 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-4 PE=2 SV=1) HSP 1 Score: 3050.77 bits (7908), Expect = 0.000e+0 Identity = 1553/1553 (100.00%), Postives = 1553/1553 (100.00%), Query Frame = 2
Query: 116 MTMQFQXXXXXXXXXXXXXXXRRGANQKSFNVKFHSIQSFHNIITTVRTSNLSEINNSLYKHSLVNSFDLDDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYXXXXXXXXXXXXXXXVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 4774
MTMQFQIISINALIISLLIDLRRGANQKSFNVKFHSIQSFHNIITTVRTSNLSEINNSLYKHSLVNSFDLDDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN
Sbjct: 1 MTMQFQIISINALIISLLIDLRRGANQKSFNVKFHSIQSFHNIITTVRTSNLSEINNSLYKHSLVNSFDLDDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 1553
BLAST of NPHS1-4 vs. TrEMBL
Match: A0A4S2LBK4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 441.81 bits (1135), Expect = 1.003e-124 Identity = 350/1348 (25.96%), Postives = 585/1348 (43.40%), Query Frame = 2
Query: 326 DDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTK--HSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVV-------SETNQPEVSI---------TSLSNNGSQIELRIDQLKSNDKIQCRVENKASRM---IRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPIS-----------------------------LKLFQNFKLDSSNGNSV--------EIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQ--TNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQIL-ENTVIYLSCETDSSNPASDIQWWVIRCSFLDGP--------------------RLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLES-----------NSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNP-YSTANFQW-----KLSFPSDI-LAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQ------CFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQF-DISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINM--LTFVI 4042
D +D+A ++QS CT E Q+++QCF+ LPQD+Y V ++V ++CVV NQHGKVQWRA+ ++LGYDRS+PG R IIGD +G+H LQI ++Q ED+GEYECQV PV H LLR+K ++ V V PSVP I + D+ LII+ + + +V ++ + C T G F W N Q + S P+ T S++ L LK D++ C V N A+++ I++R L+ + I ++ PG P+I+ S + + E L VC SP G+P + W P LK ++ S++ S +++ T++ KD+L +V R+++ +HAA L C + + + +++ I+VKY P+ V I S D S E + S + + + G+ C T+ ++ NI W ++ + Q++I+ K S V++K ++E + + C W + + A +R+ I Y P P ++GY + + ++ + C T G P P+L W+++ L NAE V+ G + S L R+DNG CAARN ATGN GI+S+++ + +FP + V + F ++ + +SC+ DSSNP + I WW RC GP R C KL+ V G G + S+L W + +++EC ++ P + + +DRL+LN+ F P F Q V + +NL + + NP + W R + +++ RL + L + ++R H NY++VA NDI ++ +FF++V + + G + +++ C+ +NP +T F W +P D+ A S N ++ P D I + H T+ + I C +N + KN F H P + ++ E VT TC+ + P + W +++ M R + T +IRP Y++ + CQA N G V + S P+ P N++ L T+N L + W QGF+GG Q+F+++ G + + ++ D++ N + +++ + I+GL S + + V S N + D I+ R EA + + ++ + ++ VA CVFGFI+LIIN+ +TF++
Sbjct: 36 DSVDAARVIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIIS---QPDPIV--RITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQR-----NLTTQVVIRVHTPPGPPRII--SPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPV-GSAHQTSIEIFVKYAPANVTI-------SVDGGSVREELV---SGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVN---FHGPSVVPSGQPLVISCQADSSNPVATINWWHFRC----GPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTENP---EYGPPVWHDRLQLNITFAPQFLGLQTRKEQVIREGTTVNLDL--GPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGE-LLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVTLVCQATANPSIATQAFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRD-----------------DSIHMTSRLSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGW-----LKAGQMIYDSQR-----EGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMNAKHGASAHTD------------FIHVRTEAFDREPGSPLVPPLSSAGPSD---DNALLIIVAACVFGFIVLIINLVVVTFLV 1305
BLAST of NPHS1-4 vs. TrEMBL
Match: A0A4S2LB20 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 441.81 bits (1135), Expect = 1.169e-124 Identity = 350/1348 (25.96%), Postives = 585/1348 (43.40%), Query Frame = 2
Query: 326 DDLDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTK--HSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVV-------SETNQPEVSI---------TSLSNNGSQIELRIDQLKSNDKIQCRVENKASRM---IRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPIS-----------------------------LKLFQNFKLDSSNGNSV--------EIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQ--TNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQIL-ENTVIYLSCETDSSNPASDIQWWVIRCSFLDGP--------------------RLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLES-----------NSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNP-YSTANFQW-----KLSFPSDI-LAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQ------CFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQF-DISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINM--LTFVI 4042
D +D+A ++QS CT E Q+++QCF+ LPQD+Y V ++V ++CVV NQHGKVQWRA+ ++LGYDRS+PG R IIGD +G+H LQI ++Q ED+GEYECQV PV H LLR+K ++ V V PSVP I + D+ LII+ + + +V ++ + C T G F W N Q + S P+ T S++ L LK D++ C V N A+++ I++R L+ + I ++ PG P+I+ S + + E L VC SP G+P + W P LK ++ S++ S +++ T++ KD+L +V R+++ +HAA L C + + + +++ I+VKY P+ V I S D S E + S + + + G+ C T+ ++ NI W ++ + Q++I+ K S V++K ++E + + C W + + A +R+ I Y P P ++GY + + ++ + C T G P P+L W+++ L NAE V+ G + S L R+DNG CAARN ATGN GI+S+++ + +FP + V + F ++ + +SC+ DSSNP + I WW RC GP R C KL+ V G G + S+L W + +++EC ++ P + + +DRL+LN+ F P F Q V + +NL + + NP + W R + +++ RL + L + ++R H NY++VA NDI ++ +FF++V + + G + +++ C+ +NP +T F W +P D+ A S N ++ P D I + H T+ + I C +N + KN F H P + ++ E VT TC+ + P + W +++ M R + T +IRP Y++ + CQA N G V + S P+ P N++ L T+N L + W QGF+GG Q+F+++ G + + ++ D++ N + +++ + I+GL S + + V S N + D I+ R EA + + ++ + ++ VA CVFGFI+LIIN+ +TF++
Sbjct: 36 DSVDAARVIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIIS---QPDPIV--RITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQR-----NLTTQVVIRVHTPPGPPRII--SPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPV-GSAHQTSIEIFVKYAPANVTI-------SVDGGSVREELV---SGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVN---FHGPSVVPSGQPLVISCQADSSNPVATINWWHFRC----GPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTENP---EYGPPVWHDRLQLNITFAPQFLGLQTRKEQVIREGTTVNLDL--GPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGE-LLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVTLVCQATANPSIATQAFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRD-----------------DSIHMTSRLSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGW-----LKAGQMIYDSQR-----EGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMNAKHGASAHTD------------FIHVRTEAFDREPGSPLVPPLSSAGPSD---DNALLIIVAACVFGFIVLIINLVVVTFLV 1305
BLAST of NPHS1-4 vs. TrEMBL
Match: A0A4S2LBR4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 441.425 bits (1134), Expect = 3.178e-124 Identity = 356/1375 (25.89%), Postives = 598/1375 (43.49%), Query Frame = 2
Query: 257 RTSNLSEINNSLYKHSLVNSFDLDD----LDSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTK--HSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVV-------SETNQPEVSI---------TSLSNNGSQIELRIDQLKSNDKIQCRVENKASRM---IRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPIS-----------------------------LKLFQNFKLDSSNGNSV--------EIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQ--TNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQIL-ENTVIYLSCETDSSNPASDIQWWVIRCSFLDGP--------------------RLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLES-----------NSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNP-YSTANFQW-----KLSFPSDI-LAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQ------CFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQF-DISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINM--LTFVI 4042
R+ L +I++S+ H L++ D +D+A ++QS CT E Q+++QCF+ LPQD+Y V ++V ++CVV NQHGKVQWRA+ ++LGYDRS+PG R IIGD +G+H LQI ++Q ED+GEYECQV PV H LLR+K ++ V V PSVP I + D+ LII+ + + +V ++ + C T G F W N Q + S P+ T S++ L LK D++ C V N A+++ I++R L+ + I ++ PG P+I+ S + + E L VC SP G+P + W P LK ++ S++ S +++ T++ KD+L +V R+++ +HAA L C + + + +++ I+VKY P+ V I S D S E + S + + + G+ C T+ ++ NI W ++ + Q++I+ K S V++K ++E + + C W + + A +R+ I Y P P ++GY + + ++ + C T G P P+L W+++ L NAE V+ G + S L R+DNG CAARN ATGN GI+S+++ + +FP + V + F ++ + +SC+ DSSNP + I WW RC GP R C KL+ V G G + S+L W + +++EC ++ P + + +DRL+LN+ F P F Q V + +NL + + NP + W R + +++ RL + L + ++R H NY++VA NDI ++ +FF++V + + G + +++ C+ +NP +T F W +P D+ A S N ++ P D I + H T+ + I C +N + KN F H P + ++ E VT TC+ + P + W +++ M R + T +IRP Y++ + CQA N G V + S P+ P N++ L T+N L + W QGF+GG Q+F+++ G + + ++ D++ N + +++ + I+GL S + + V S N + D I+ R EA + + ++ + ++ VA CVFGFI+LIIN+ +TF++
Sbjct: 85 RSVQLQKISDSI-AHPLLSDPLFDPSILTVDAARVIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIIS---QPDPIV--RITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQR-----NLTTQVVIRVHTPPGPPRII--SPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPV-GSAHQTSIEIFVKYAPANVTI-------SVDGGSVREELV---SGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVN---FHGPSVVPSGQPLVISCQADSSNPVATINWWHFRC----GPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTENP---EYGPPVWHDRLQLNITFAPQFLGLQTRKEQVIREGTTVNLDL--GPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGE-LLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVTLVCQATANPSIATQAFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRD-----------------DSIHMTSRLSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGW-----LKAGQMIYDSQR-----EGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMNAKHGASAHTD------------FIHVRTEAFDREPGSPLVPPLSSAGPSD---DNALLIIVAACVFGFIVLIINLVVVTFLV 1380
BLAST of NPHS1-4 vs. TrEMBL
Match: G7YEL7 (Nephrin OS=Clonorchis sinensis OX=79923 GN=CLF_106049 PE=4 SV=1) HSP 1 Score: 425.246 bits (1092), Expect = 4.687e-119 Identity = 341/1321 (25.81%), Postives = 575/1321 (43.53%), Query Frame = 2
Query: 335 DSAVLLQSKKCTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTK--HSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVV-------SETNQPEVSI---------TSLSNNGSQIELRIDQLKSNDKIQCRVENKASRM---IRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPIS-----------------------------LKLFQNFKLDSSNGNSV--------EIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFFESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQ--TNSFVELKATKEINNMDLLCTAWPVDSVILSASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQIL-ENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPR----------------LVKRLIP----CSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHF-SKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLES-----------NSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNP-YSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQ-CFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCT-FVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQF-DISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGF 4006
D+A ++QS CT E Q+++QCF+ LPQD+Y V ++V ++CVV NQHGKVQWRA+ ++LGYDRS+PG R IIGD +G+H LQI ++Q ED+GEYECQV PV H LLR+K ++ V V PSVP I + D+ LII+ + + +V ++ + C T G P F W N Q + S P+ T S++ L LK D++ C V N A+++ I++R L+ + I ++ PG P+I+ S + + E L VC SP G+P + W P LK ++ S++ S +++ T++ KD+L +V R+++ +HAA L C + + + +++ I+VKY P+ V I S + S E + S + + + G+ C T+ ++ NI W ++ + Q++I+ K S V++K + E + + C W + + A +R+ I Y P P ++GY + + ++ + C T G P P+L W+++ L N+E V+ G + S L R+DNG CAARN ATGN GI+S ++ + +FP + V + F ++ + +SC+ DSSNP + I WW RC GP L +R P C KL+ V G G + S+L W + +++EC ++ P + + +DRL+LN+ F P F Q V + +NL + + NP + W R + +++ RL + L + ++R H NY++VA NDI ++ +FF++V + + G + +++ C+ +NP + +F W P N E ++ C + ++ K F + C S F S ++ ++ C +N + KN F H AP + ++ E VT TC+ + P + W + + E + + T +IRP Y++ + CQA N G V + S P+ P N++ L T+N L + W QGF+GG Q+F+++ G + + ++ D++ N + +++ + I+GL S + + V S N + D I+ R EA + + ++ + ++ VA CVFGF
Sbjct: 38 DAARVIQSSACTPQERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIIS---QPDPIV--RITVLCVTVGGSPPPSFTWIINNQEIPTKPLQTSAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCGVTNAATQLHHDIQQR-----NLTTQVMIRVHTPPGPPRII--SPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPAQVGDQKTGRIIGFGGRGGARMNEYSAVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPV-GSAHQTSIEIFVKYAPANVTI-------SVEGGSVREELV---SGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISDEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRNLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNSEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASEAVVLNVVFPPQGVSVN---FHGPSVVPSGQPLVISCQADSSNPVAIINWWHFRC----GPAHAFSQFKASKTTVDEVLYERRTPERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTENP---EYGPPVWHDRLQLNITFAPQFLGLQTRKEHVIREGTTVNLDL--GPYSNPPVTSVSWFVEGRPIPPETAPAAARTGVHTSGRLGE-LLALHKISRTHMANYTVVARNDIAESSAIFFLNVTFPASLAGAAEENVTQTTSDQVTLVCQATANPSIAIQSFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDAGPSV---DDKYF--IHCQRDDSVHMTSRF-SIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFAPGIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLKAGQIIHDSQREGSGA-----------STQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMNAKHGASAHTD------------FIHVRTEAFDREPGSPLVPPLSSAGPSD---DNALLIIVAACVFGF 1290
BLAST of NPHS1-4 vs. Ensembl Cavefish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 108.227 bits (269), Expect = 4.932e-23 Identity = 117/465 (25.16%), Postives = 181/465 (38.92%), Query Frame = 2
Query: 1547 ASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENSREQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILS-ASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLR----LNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWL-RGHRVLESNSNFRLFQN--TLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
S ++G C T+ ++I W L KE N + SE G +N + ++E N++ L C A+ + S S + +YY P K L + T+ L+C + G P L W K N T+ V + +S + DN +C A N A I S I + FPA VKI + K++ + L C SSNP ++I W + +L + + E+ G SKL + + V C+ A SSL + + LNV+F P F++ P S Q E+ T+ V NP+ WL G +V++ + F L I+N+TR GNY + N GK + +DV+Y+P V + + V + CE +NP + F WK
Sbjct: 360 GSFEAVEGNQVTLCCHTSSSNPPVHIRWWLGFKE-----LNNATVTISEGENGGMMTMSN--LTYTVSREENSLPLTCEAFNRGTRYSSIKSQPLSVYYPPQKVWLDAPPEGTLLRSGTTVRLVCFSSGGNPTGRLTWMKNNKTVQVPSKHVESERGVSREFLLTLQPSDNLATYRCDATNEAK---EIRSAQIKLLVQFPAVSVKIL---VKQKELRAGRSLSLDCLAGSSNPKANISWSL------------------GSTRLNGVEQAPKRAEFGGLSVSSKLTLPLGSEHHGKRVSCQ--------AFSSLLSEGVNTFYVLNVLFPPEFAEDQP--SEVQIVEDDTATLPIMVSANPEDFTCEWLFHGEKVVKDRDHRYHFPEGWALEIWNVTRRDAGNYRVECSNAEGKRSSIIKLDVQYSPT-VKMKTDPVFVNVGDTADLMCEADANPITPGMFSWK 782 HSP 2 Score: 71.633 bits (174), Expect = 7.051e-12 Identity = 68/278 (24.46%), Postives = 114/278 (41.01%), Query Frame = 2
Query: 1844 PNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVS---GEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPH--FSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLE 2662
P P + G E + ++ L+C + G PL L W K LS V K SS L+ +V EDNG ++ C + N +S + +I + + T + +E + L C T SSNP I+WW + KEL+ V+ GE G T+S L Y+V +N + CE+ ++ T L+V + P + P G++ ++ + L + F+ GNP R W++ ++ ++
Sbjct: 255 PQAPVIEGLRSQE-VKAGTSLRLVCLSYGGNPLATLHWTKNGEVLSTNWEVDVVSQKASSGLNMQVQPEDNGAVLCCESVN------QVSRSPQSITRTLTVLFEPAEVTLLGSFEAVEGNQVTLCCHTSSSNPPVHIRWW---------------------LGFKELNNATVTISEGENGGMMTMSNLTYTVSREENSLPLTCEA----FNRGTRYSSIKSQPLSVYYPPQKVWLDAPPEGTLLRSGTTVRL-VCFSSGGNP-TGRLTWMKNNKTVQ 498 HSP 3 Score: 70.8626 bits (172), Expect = 1.386e-11 Identity = 37/112 (33.04%), Postives = 62/112 (55.36%), Query Frame = 2
Query: 395 QCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYF 727
Q F P++ V ++ ++KC V G VQW ++LG RS+PG R ++ GD +G+++LQI +++ ED YECQV + T ++ + + V + P+ P YF
Sbjct: 43 QAFRTEPRN-LTVRTGATALLKCEVLRASGSVQWVKDGLLLGPQRSLPGHPRYSMTGDQDRGQYHLQIEDVRLEDDSPYECQVGRSKTSRPIVSRTVWLNVQIPPAEP--YF 151 HSP 4 Score: 51.6026 bits (122), Expect = 8.089e-6 Identity = 30/112 (26.79%), Postives = 59/112 (52.68%), Query Frame = 2
Query: 3497 CQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFAD 3832
C A N G D+ ++++ S P+PP +++ L + S+ + ++W GF+GG Q+FR++ E + D+ R S + ++GL + V + N++ + S+AD
Sbjct: 923 CTARNSQGEDNLDIQLLSTSYPDPPSDLKLLSVASDSVTLEWMPGFDGGLIQNFRVRYQWAES-ASYLYVDVFPPR---STVYTVTGLKPVTAYNFSVNAINSM-GESSYAD 1029
BLAST of NPHS1-4 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 84.7297 bits (208), Expect = 4.637e-16 Identity = 66/230 (28.70%), Postives = 98/230 (42.61%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI----YFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITI 1060
F PQD+ V+ SV + CV+ G VQW + LG +R +PG R +++GD + GEH+L I + + D YECQ T+ L +A++TV V PS PV+ R K T H L C G + WY + +V+ + SL +G S + + + S CRV N A+ R + SVTI++
Sbjct: 15 FSQQPQDQV-VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQ---ATQAGLRSHRARLTVLVPPSDPVVDGGPVVRLKAHTPHNLT------------------CRATGAKPAAEITWYRDGEVMHTA----IYSKSLMEDGKRETAVSLLPMTPEDRDSGRTYTCRVLNPAAPAGR-------QTSVTISV 211
BLAST of NPHS1-4 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 84.7297 bits (208), Expect = 5.713e-16 Identity = 66/230 (28.70%), Postives = 98/230 (42.61%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI----YFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITI 1060
F PQD+ V+ SV + CV+ G VQW + LG +R +PG R +++GD + GEH+L I + + D YECQ T+ L +A++TV V PS PV+ R K T H L C G + WY + +V+ + SL +G S + + + S CRV N A+ R + SVTI++
Sbjct: 18 FSQQPQDQV-VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQ---ATQAGLRSHRARLTVLVPPSDPVVDGGPVVRLKAHTPHNLT------------------CRATGAKPAAEITWYRDGEVMHTA----IYSKSLMEDGKRETAVSLLPMTPEDRDSGRTYTCRVLNPAAPAGR-------QTSVTISV 214
BLAST of NPHS1-4 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 84.7297 bits (208), Expect = 6.256e-16 Identity = 66/230 (28.70%), Postives = 98/230 (42.61%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVI----YFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITI 1060
F PQD+ V+ SV + CV+ G VQW + LG +R +PG R +++GD + GEH+L I + + D YECQ T+ L +A++TV V PS PV+ R K T H L C G + WY + +V+ + SL +G S + + + S CRV N A+ R + SVTI++
Sbjct: 53 FSQQPQDQV-VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQ---ATQAGLRSHRARLTVLVPPSDPVVDGGPVVRLKAHTPHNLT------------------CRATGAKPAAEITWYRDGEVMHTA----IYSKSLMEDGKRETAVSLLPMTPEDRDSGRTYTCRVLNPAAPAGR-------QTSVTISV 249
BLAST of NPHS1-4 vs. Ensembl Cavefish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 73.9442 bits (180), Expect = 1.093e-12 Identity = 70/264 (26.52%), Postives = 107/264 (40.53%), Query Frame = 2
Query: 365 CTNFEIQEKLQCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSET---------NQPEVSITSLSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFE 1129
C F + F P D V+ V + C + HG V W + LG +R + G R ++IGD SKGE++L I + D G +ECQ + + + A++TV V P PVI I P+ + L L C + IW N +V++ Q E ++++L + S IE S +I CR NKA+ ++ T+TI P ++NLSV + E
Sbjct: 19 CLCFCVSGACAVFVQQPHDVV-VVAGQPVTLPCSITGFHGMVLW--DGLALGVNRDLSGYPRYDVIGDHSKGEYHLLIQRTELGDDGSFECQ---GIQAGIRSRPARLTVLVPPDDPVI------------IGAPI--VSLRAGDPLNLTCHADNAKPAASIIWIRNGEVLNGAMYSKTLLRDGQRESTVSTLYISASNIE-------SGQRITCRASNKAAPNGKE---------ATVTIDIQHLP----LVNLSVEPQPILE 242
BLAST of NPHS1-4 vs. Ensembl Sea Lamprey
Match: cadm2a (cell adhesion molecule 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1614]) HSP 1 Score: 53.9138 bits (128), Expect = 1.175e-7 Identity = 49/204 (24.02%), Postives = 81/204 (39.71%), Query Frame = 2
Query: 2192 ILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYA 2803
++E I L+C++ S PA++I+W+ +LK RE + IS + +V+W+DN V C+ P ++L L+V + P P+ +F EE + + GNP + W R R E R+ N L I L + G Y N+IG + + + V+ A
Sbjct: 9 LMEGDTIQLTCQSTGSKPAANIRWFK------------------GNQELKGQRREKKDNNKKTFTVISTVNITVEWVDNMTPVRCQMDHP---ALRTTLLQTSQDLHVQYKPRLVIT-PSSDIF--EEGDTMELSCIATGNPNPSSIQWAR--RDGEMPDRARILGNKLVITALNKTDNGTYKCEGFNNIGHSAEEYTLYVREA 186
BLAST of NPHS1-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004946.1 (pep scaffold:Pmarinus_7.0:GL501092:7822:21335:1 gene:ENSPMAG00000004486.1 transcript:ENSPMAT00000004946.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 48.521 bits (114), Expect = 9.800e-6 Identity = 70/301 (23.26%), Postives = 124/301 (41.20%), Query Frame = 2
Query: 1916 CSTKNGYPLPDLVWYKEN-----ATLSNAE--PVVSNGDK----ISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTI---FPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSS-------LHY-DRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAH 2752
C K+GYP P +VW+K + + NAE PVV+ ++ ++S+L + + D G +I C GN I + + TI +P V ++ P +K E + L+C TD+ P + +F G L+ + K+ ++R G Y +CE++ +S T++ +HY +RLR+ I P+G V + +N+ +T + GN + W ++ E + R+ L ++T Y G+Y+ VA
Sbjct: 162 CVAKDGYPEPKIVWFKNSNPIIEVKIENAEVMPVVTKNNQGLFTVTSVLRMRPQKTDKGSLINCCVMT-GGGNTEIEAPLATMVTIHLSYPPEGVSLEVFPNFIK---EGDTVTLTCVTDAFPPPT--------YAFFQGETLLAETGNKNTFKMPYINRR-QGGVY----------------------KCEARYTSSPMQTTAKAERQINVHYIERLRIMGI---------PDGEVNKG-DNITMTCSASSSGNIT---YHW---NKDGERKEDGRI----LTRLHVTSYDSGSYTCVAQ 407
BLAST of NPHS1-4 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 144.436 bits (363), Expect = 2.869e-34 Identity = 205/862 (23.78%), Postives = 343/862 (39.79%), Query Frame = 2
Query: 395 QCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVP------VIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGL-VTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFF---ESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENS-----REQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILS-ASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTI-SKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFR---LFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
Q F P++ V ++ V+KC V G VQW ++LG RS+PG R ++IG +G+++L+I N Q ED YECQ Q T ++ + V + PS P Y+ + D T + P K P+ + + +G+ +T ++ + EV++T+LS++ ++ + C +N A + K + + T+T+ Y P P I+NL + L + C VS G+P+ +R W S NG EI S D F + S +++ + ++ + A L+C +S + +S S + F E + + S+ I+GQ +C ++I W L KE + RE+ DN + + S V + ++E N + L C A+ + +I +YY P K L +N ++L+C + G P P L W K +++A S+ + L ++ DN +C A N A S + F A +KI + L+Q V+ L C +SSNP ++I W V F K ++ H S + SG T+ S L ++ ++ V C++ P ++ ++ + RLNV+ P FS P + Q EE+ TI V NP+ VWL L +N L +L I N TR G Y++ N+ G ++ +DV YA + E++ + V + C +NP + F WK
Sbjct: 27 QAFRIEPRN-LTVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIG--KRGQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPYFTMDMAAYWVAGESYDVT-CVAPDAK------PEAEVILYKDGVKLTGVESSSVSGSKDKLLNTLAEVTVTALSSDNGRL------------LACHAKNAA---LSKPVVT------TVTMNVYYPPDAPFIVNL---DRDEVRAGRMLMLEC-VSGSGNPLAALR--W----------------SKNG---EIVSTTWEADEFARK-SRSVLKMVITPADNQAELSC-----------------------------ESFNAVSLSPLSITRKLDVFFDPEELTLSGSLEAIEGQEVVLSCFAASSNPPVDIRWWLGYKELNNTVFIREEG--DNGGMTTV----------SNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPSDNMATYRCDATNNAKKTI---SAHAKLLVYFTAVSMKITAHQEVLRQ---GEVLTLECLAESSNPTANIFWSVGAVRFQ---------------KAEQTH----SKDKSGAVTVNSTLLLNLTSQHHKQNVICQAYNPVLEEGANTYY----RLNVLHPPEFSSAQP--TEIQVEEDEMATIPLLVSANPEGVSCVWLHRRETLVKGNNLHHYWLEDGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVF-VNLGGTADLICVADANPIISDMFSWK 758
BLAST of NPHS1-4 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 138.658 bits (348), Expect = 2.410e-32 Identity = 202/852 (23.71%), Postives = 331/852 (38.85%), Query Frame = 2
Query: 395 QCFEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQV-QPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNGSQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIYWNFNPISLKLFQNFKLDSSNGNSVEIKSQRITIDSFKDELSGNIVDLRLSKWHHAANLTCFIKQLLLKNNYFSTVRIYVKYPPSVVKIYQSSDPISTDHFSYDEYISFF---ESIKTNASISLIDGQFYRFACSTNLHYTTLNINWSLAMKENS-----REQYNLDNIAQSEIIVGLYFKQTNSFVELKATKEINNMDLLCTAWPVDSVILS-ASIRIKIYYSPNKPTLSGYTIAEAINEHETISLLCSTKNGYPLPDLVWYKENATLSNAEPVVSNGDKISSLLSFKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTI-SKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWK 2917
Q F P++ V ++ V+KC V G VQW ++LG RS+PG R ++IG +G+++L+I N Q ED YECQ Q T ++ + V + PS P YF + + + + + K E F I+ + V SE +T+LS++ ++ + C +N A + K + + T+T+ Y P P I+NL + L + C VS G+P+ +R W S NG EI S D F + S +++ + ++ + A L+C +S + +S S + F E + + S+ I+GQ +C ++I W L KE + RE+ DN + + S V + ++E N + L C A+ + +I +YY P K L +N ++L+C + G P P L W K +++A S+ + L ++ DN +C A N A S + F A +KI + L+Q V+ L C +SSNP ++I W V F V L H+ SG T+ S L ++ ++ V C++ P ++ ++ + RLNV+ P FS P + Q EE+ TI V NP+ VWL L +L I N TR G Y++ N+ G ++ +DV YA + E++ + V + C +NP + F WK
Sbjct: 50 QAFRIEPRN-LTVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIG--KRGQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNP--YFTMD-MAAYWVAGESYDVTCVAPDAKPEAEHF-------FVCIFTFGSCVTSE------RVTALSSDNGRL------------LACHAKNAA---LSKPVVT------TVTMNVYYPPDAPFIVNL---DRDEVRAGRMLMLEC-VSGSGNPLAALR--W----------------SKNG---EIVSTTWEADEFARK-SRSVLKMVITPADNQAELSC-----------------------------ESFNAVSLSPLSITRKLDVFFDPEELTLSGSLEAIEGQEVVLSCFAASSNPPVDIRWWLGYKELNNTVFIREEG--DNGGMTTV----------SNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPSDNMATYRCDATNNAKKTI---SAHAKLLVYFTAVSMKITAHQEVLRQ---GEVLTLECLAESSNPTANIFWSVGAVRF--------------GVTLSTKHQ-------SGAVTVNSTLLLNLTSQHHKQNVICQAYNPVLEEGANTYY----RLNVLHPPEFSSAQP--TEIQVEEDEMATIPLLVSANPEGVSCVWLHRRETLVKGDP----DGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVF-VNLGGTADLICVADANPIISDMFSWK 756
BLAST of NPHS1-4 vs. Ensembl Medaka
Match: kirrel3l (kirre like nephrin family adhesion molecule 2 [Source:NCBI gene;Acc:101173407]) HSP 1 Score: 87.4261 bits (215), Expect = 8.269e-17 Identity = 86/303 (28.38%), Postives = 128/303 (42.24%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQ-KKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITS--LSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKLFQNFKLDSSNGNSVEI 1279
F PQD+ V+ SV + CV+ G VQW + LG +R +PG R +++GD GEH+L I + + D YECQ T+ L +A++TV V PS PV+ +L H TP N L C +G + WY + +V+ E +I S L +G S + + + + CRV N A+ R + SVTI + P + LSV V E + L +C S PEI Y W+ + +NG+S+E+
Sbjct: 20 FSQQPQDQV-VVSGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLMGDPLSGEHSLLIDSAELADDAVYECQ---ATQAGLRSHRAKLTVLVPPSDPVVEGGPVVRLKAH----TPHN-----------LTCRASGAKPAAEITWYRDGEVM------ETAIYSKTLMQDGKRETAVSMLPIIPEDSDTGRTYTCRVLNPAAPAGR-------QTSVTINVQ------HPPSVTLSVQPQTVTEGAKVL-FLCSASAN----PEITGYRWSKG--------GVPISEANGDSLEV 271
BLAST of NPHS1-4 vs. Ensembl Medaka
Match: kirrel3l (kirre like nephrin family adhesion molecule 2 [Source:NCBI gene;Acc:101173407]) HSP 1 Score: 87.0409 bits (214), Expect = 1.040e-16 Identity = 86/303 (28.38%), Postives = 128/303 (42.24%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQ-KKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITS--LSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFEDKETLNMVCRVSPVGSPIPEIRIY-WNFNPISLKLFQNFKLDSSNGNSVEI 1279
F PQD+ V+ SV + CV+ G VQW + LG +R +PG R +++GD GEH+L I + + D YECQ T+ L +A++TV V PS PV+ +L H TP N L C +G + WY + +V+ E +I S L +G S + + + + CRV N A+ R + SVTI + P + LSV V E + L +C S PEI Y W+ + +NG+S+E+
Sbjct: 19 FSQQPQDQV-VVSGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLMGDPLSGEHSLLIDSAELADDAVYECQ---ATQAGLRSHRAKLTVLVPPSDPVVEGGPVVRLKAH----TPHN-----------LTCRASGAKPAAEITWYRDGEVM------ETAIYSKTLMQDGKRETAVSMLPIIPEDSDTGRTYTCRVLNPAAPAGR-------QTSVTINVQ------HPPSVTLSVQPQTVTEGAKVL-FLCSA----SANPEITGYRWSKG--------GVPISEANGDSLEV 270
BLAST of NPHS1-4 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 78.9518 bits (193), Expect = 3.427e-14 Identity = 68/249 (27.31%), Postives = 104/249 (41.77%), Query Frame = 2
Query: 401 FEDLPQDRYEVLHNSSVVIKCVVRNQHGKVQWRAQNVVLGYDRSVPGMERLNIIGDASKGEHNLQISNIQPEDSGEYECQVQPVTKHSLLRKKAQVTVHVLPSVPVIYFRQKKLTDHTLIITPMNKNNYVVNPKLVLECFTNGLVTPLQFIWYWNRQVVSETNQPEVSITSLSNNG------SQIELRIDQLKSNDKIQCRVENKASRMIRKRMKSYIELSVTITILFYSFPGEPKILNLSVLNSKVFE 1129
F PQD V+ V + C + HG V W + LG R + G R +IGD GEH+L+I ++ D +ECQ + ++ + A++TV V P PVI + + + L L C + IW N +V++ +VSI +L +G S + L +++ +I CR NKA + + E SVTI I ++NLSV V E
Sbjct: 20 FSQQPQDLV-VVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQA---VQAAMRSRPARLTVLVPPDDPVIGGGP---------VVSLRAGD-----PLNLTCHADNAKPAASIIWMRNGEVLNGAMYSKVSIEALVRDGRRESTVSTLYLSPSNIETGQQIICRASNKA-------VPNGKEASVTIDIQHL------PLVNLSVEPQAVLE 237
BLAST of NPHS1-4 vs. Planmine SMEST
Match: SMESG000049955.1 (SMESG000049955.1) HSP 1 Score: 1748.79 bits (4528), Expect = 0.000e+0 Identity = 899/914 (98.36%), Postives = 906/914 (99.12%), Query Frame = 2
Query: 2033 FKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYXXXXXXXXXXXXXXXVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 4774
F + E N IIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRL+PCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNS+IHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVE NYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPEN+QCLQI SNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEV+QFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIIN+LTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPD+TLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN
Sbjct: 28 FMILIESNDFIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLVPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSVIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVEPNYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENLQCLQIKSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVVQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIINVLTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDETLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 941
BLAST of NPHS1-4 vs. Planmine SMEST
Match: SMESG000049955.1 (SMESG000049955.1) HSP 1 Score: 1748.79 bits (4528), Expect = 0.000e+0 Identity = 899/914 (98.36%), Postives = 906/914 (99.12%), Query Frame = 2
Query: 2033 FKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYXXXXXXXXXXXXXXXVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 4774
F + E N IIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRL+PCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNS+IHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVE NYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPEN+QCLQI SNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEV+QFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIIN+LTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPD+TLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN
Sbjct: 29 FMILIESNDFIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLVPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSVIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVEPNYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENLQCLQIKSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVVQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIINVLTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDETLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 942
BLAST of NPHS1-4 vs. Planmine SMEST
Match: SMESG000049955.1 (SMESG000049955.1) HSP 1 Score: 1747.64 bits (4525), Expect = 0.000e+0 Identity = 899/914 (98.36%), Postives = 906/914 (99.12%), Query Frame = 2
Query: 2033 FKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYXXXXXXXXXXXXXXXVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 4774
F + E N IIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRL+PCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNS+IHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVE NYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPEN+QCLQI SNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEV+QFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIIN+LTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPD+TLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN
Sbjct: 18 FMILIESNDFIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLVPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSVIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVEPNYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENLQCLQIKSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVVQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIINVLTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDETLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 931
BLAST of NPHS1-4 vs. Planmine SMEST
Match: SMESG000049955.1 (SMESG000049955.1) HSP 1 Score: 1660.58 bits (4299), Expect = 0.000e+0 Identity = 865/914 (94.64%), Postives = 876/914 (95.84%), Query Frame = 2
Query: 2033 FKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYXXXXXXXXXXXXXXXVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 4774
F + E N IIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRL+PCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEEN RF + + N ++ NTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNS+IHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVE NYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPEN+QCLQI SNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEV+QFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIIN+LTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPD+TLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN
Sbjct: 18 FMILIESNDFIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLVPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENFF--------------RFKLDNQIQCFRESENQQIRLNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSVIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVEPNYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENLQCLQIKSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVVQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIINVLTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDETLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 917
BLAST of NPHS1-4 vs. Planmine SMEST
Match: SMESG000049955.1 (SMESG000049955.1) HSP 1 Score: 1660.2 bits (4298), Expect = 0.000e+0 Identity = 865/914 (94.64%), Postives = 876/914 (95.84%), Query Frame = 2
Query: 2033 FKVSREDNGKIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLIPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENLNLTIKFNVFGNPKINRFVWLRGHRVLESNSNFRLFQNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSIIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVESNYMESIKSRPVXXXXXXXXXXXXXXXDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENVQCLQITSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVMQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEAXXXXXXXXXXXXXXXXXEESIQKSTSFIVTVAGCVFGFIILIINMLTFVICYXXXXXXXXXXXXXXXVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDDTLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 4774
F + E N IIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRL+PCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEEN RF + + N ++ NTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNS+IHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVE NYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPEN+QCLQI SNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEV+QFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIIN+LTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPD+TLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN
Sbjct: 29 FMILIESNDFIIKCAARNIATGNFGISSNSIAIRTIFPARYVKIKSTPFQLKQILENTVIYLSCETDSSNPASDIQWWVIRCSFLDGPRLVKRLVPCSKVKLKELHREIVSGEYSGWRTISKLKYSVKWLDNENFVECESKLPNSDKATSSLHYDRLRLNVIFGPHFSKQYPNGSVFQAEENFF--------------RFKLDNQIQCFRESENQQIRLNTLRIFNLTRYHRGNYSMVAHNDIGKTEILFFIDVKYAPVIVGERNSVIHVPFKHNISVGCEVASNPYSTANFQWKLSFPSDILAPFNNSLEWHINYTEKCPEFRDLSTIFSNSTKPFASVSCATPRKFATLSNFHSTNSVDFQNIRLQCFYHNNISSSAIKNFIFYIHSAPEFQAVSQFAFEVVESSEAVTFTCIFKGYPINSVEWHNCTFVEPNYMESIKSRPVTNNSLTNQTLLLQSTDIQQIRPNFYRAEQIFSYRDLKMLNRICCQAANRYGTDHGIVRIVLKSKPNPPENLQCLQIKSNFLLIKWDQGFNGGSDQSFRIKVSRQEGFHEVVQFDISGNRHRNSLFHNISGLLSNVCFVIRVGSFNNLFADVSFADPVIACTLSDQNISINHRVEANTKTNITNIISINPKPNEESIQKSTSFIVTVAGCVFGFIILIINVLTFVICYKKKQERIRKRRQSKEVQSTDERQTENLSKYDSSLIENHLYDGSILAASDSCFGGELRDKEEEIPMLMYQKPICPLHFVPNDQYSNPLIIQDHYLQSAISCNQTINRNETTCYGCTSIELPLNSMILSNQYLPFPLLESTYSSLMRNSCEYSLSSTSNPSDIRILNETSQVDSRALPDETLSNGFPKIPLNISAISIPSTNSSVKQKQDSYQNIFQKYSLPEEKQPIQSPTFQRGFVKNSGNN 928 The following BLAST results are available for this feature:
BLAST of NPHS1-4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of NPHS1-4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of NPHS1-4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of NPHS1-4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of NPHS1-4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of NPHS1-4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of NPHS1-4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of NPHS1-4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of NPHS1-4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of NPHS1-4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of NPHS1-4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of NPHS1-4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of NPHS1-4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of NPHS1-4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30021054 ID=SMED30021054|Name=NPHS1-4|organism=Schmidtea mediterranea sexual|type=transcript|length=4976bpback to top protein sequence of SMED30021054-orf-1 >SMED30021054-orf-1 ID=SMED30021054-orf-1|Name=SMED30021054-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1554bp MTMQFQIISINALIISLLIDLRRGANQKSFNVKFHSIQSFHNIITTVRTSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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