Tubulin alpha chain

Overview
NameTubulin alpha chain
Smed IDSMED30021022
Length (bp)1214
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tubulin alpha chain (SMED30021022) t-SNE clustered cells

Violin plots show distribution of expression levels for Tubulin alpha chain (SMED30021022) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30021022

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 52

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30021022h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30021022h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30021022h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30021022h1SMcG0000479 PL08009A2C06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
prepharyngeal regionSMED30021022h1SMcG0000478 SmedASXL_008060SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30021022h1SMcG0000478 SmedASXL_008060SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14354newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14354uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig41392newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig41392uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14476uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14476newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig45405uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig45405newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30021022h1SMcG0000479 dd_Smed_v4_18010_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14354newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14354uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig41392newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig41392uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14476uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig14476newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig45405uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30021022h1SMcG0000479 Contig45405newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30021022h1SMcG0000479 dd_Smed_v4_18010_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30021022h1SMcG0000479 dd_Smed_v4_6577_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30021022h1SMcG0000479 dd_Smed_v6_60553_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30021022h1SMcG0000479 dd_Smed_v6_18010_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30021022h1SMcG0000479 dd_Smed_v6_60553_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30021022h1SMcG0000479 dd_Smed_v6_18010_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
gutSMED30021022h1SMcG0000479 SMED30000639smed_20140614PMID:26457503
Tu et al., 2015
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30021022h1SMcG0000479 SMED30000639smed_20140614PMID:26457503
Tu et al., 2015
whole organism asexual adult colorimetric in situ hybridization evidence
gamma neoblastSMED30021022h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30021022h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30021022h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30021022h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30021022h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30021022h1SMcG0000479 dd_Smed_v6_6577_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 234.572 bits (597), Expect = 4.526e-75
Identity = 126/251 (50.20%), Postives = 174/251 (69.32%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF +               +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFAS---------------LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.781 bits (164), Expect = 4.526e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1C (tubulin alpha 1c [Source:HGNC Symbol;Acc:HGNC:20768])

HSP 1 Score: 234.572 bits (597), Expect = 4.998e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.998e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA1B (tubulin alpha 1b [Source:HGNC Symbol;Acc:HGNC:18809])

HSP 1 Score: 234.572 bits (597), Expect = 5.297e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.297e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 234.187 bits (596), Expect = 6.150e-75
Identity = 126/251 (50.20%), Postives = 174/251 (69.32%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF +               +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFAS---------------LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 6.150e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Human
Match: TUBA3D (tubulin alpha 3d [Source:HGNC Symbol;Acc:HGNC:24071])

HSP 1 Score: 234.187 bits (596), Expect = 6.150e-75
Identity = 126/251 (50.20%), Postives = 174/251 (69.32%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF +               +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFAS---------------LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 6.150e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-2 (Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P34690])

HSP 1 Score: 239.195 bits (609), Expect = 2.004e-76
Identity = 118/247 (47.77%), Postives = 171/247 (69.23%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  +H+GQ G+QIG A WEL   EHGI  DG M     +E  S+ T+F+ + +G+YVPRSIFVDLEPTVVD++RTG Y++L+HP Q+I G EDAANN+ARG Y++GK+ +  V+   R+ A+NC  +  F + HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNR+++QV
Sbjct:    1 MREVISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPTQSTNEGESFTTFFSDTGSGRYVPRSIFVDLEPTVVDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDVERPSYTNLNRIISQV 232          

HSP 2 Score: 66.2402 bits (160), Expect = 2.004e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  231 QVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISAEK 278          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-2 (Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P34690])

HSP 1 Score: 239.195 bits (609), Expect = 2.004e-76
Identity = 118/247 (47.77%), Postives = 171/247 (69.23%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  +H+GQ G+QIG A WEL   EHGI  DG M     +E  S+ T+F+ + +G+YVPRSIFVDLEPTVVD++RTG Y++L+HP Q+I G EDAANN+ARG Y++GK+ +  V+   R+ A+NC  +  F + HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNR+++QV
Sbjct:    1 MREVISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPTQSTNEGESFTTFFSDTGSGRYVPRSIFVDLEPTVVDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDVERPSYTNLNRIISQV 232          

HSP 2 Score: 66.2402 bits (160), Expect = 2.004e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  231 QVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISAEK 278          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-2 (Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P34690])

HSP 1 Score: 239.195 bits (609), Expect = 2.004e-76
Identity = 118/247 (47.77%), Postives = 171/247 (69.23%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  +H+GQ G+QIG A WEL   EHGI  DG M     +E  S+ T+F+ + +G+YVPRSIFVDLEPTVVD++RTG Y++L+HP Q+I G EDAANN+ARG Y++GK+ +  V+   R+ A+NC  +  F + HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNR+++QV
Sbjct:    1 MREVISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPTQSTNEGESFTTFFSDTGSGRYVPRSIFVDLEPTVVDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDVERPSYTNLNRIISQV 232          

HSP 2 Score: 66.2402 bits (160), Expect = 2.004e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  231 QVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISAEK 278          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-4 (Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:G5EDD4])

HSP 1 Score: 236.113 bits (601), Expect = 7.561e-76
Identity = 118/247 (47.77%), Postives = 170/247 (68.83%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  +H+GQ G+QIG A WEL   EHGI  DG M +   +E  S+ T+F+ +  G+YVPRSIFVDLEPTVVD++RTG Y++L+HP Q+I G EDAANN+ARG Y++GK+ +   +   R+ A+NC  +  F + HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD D  ++TNLNR+++QV
Sbjct:    1 MREVISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSEQQNEGGSFTTFFSDTGNGRYVPRSIFVDLEPTVVDEIRTGNYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTTLDRIRRLADNCSGLQGFFVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICKRNLDVDRPSYTNLNRIISQV 232          

HSP 2 Score: 67.3958 bits (163), Expect = 7.561e-76
Identity = 28/48 (58.33%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL S+ P +   K
Sbjct:  231 QVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLASYTPLISAEK 278          
BLAST of Tubulin alpha chain vs. Ensembl Celegans
Match: tba-9 (Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:Q20221])

HSP 1 Score: 233.417 bits (594), Expect = 2.669e-75
Identity = 123/251 (49.00%), Postives = 176/251 (70.12%), Query Frame = 2
Query:   56 KMHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGD---VDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            +  ++  +H+GQ G+Q+G A WEL   EHGI  DG++N  D   VD+  S+NT+F+ +MAG++VPR+I VDLEPT +D++RTG Y+ L+HP QL+ G EDAANN+ARG Y+IGK+ +  V+   R+  ENC  +  FL+ HSFGGGTGSGFTA               +LME LSV+YGK++K+E  ++P+P +STS+VEPYN++L  H  +E+SD +   +NE+++ +C  NLD    T+TNLNRL+AQV
Sbjct:    5 RSREVISIHVGQAGVQMGNACWELYCLEHGIQPDGMINEEDSLGVDDD-SFNTFFSETMAGKHVPRAIMVDLEPTPIDEIRTGTYKTLFHPEQLLTGKEDAANNYARGHYTIGKEIIDVVMDRVRRLVENCRGLQGFLVFHSFGGGTGSGFTA---------------LLMERLSVDYGKKSKLEFCIYPAPQVSTSMVEPYNSILTTHTTLEHSDCSFMVDNEAIYDICRRNLDLARPTYTNLNRLIAQV 239          

HSP 2 Score: 68.5514 bits (166), Expect = 2.669e-75
Identity = 28/48 (58.33%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q+IS  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  238 QVISSVTASLRFDGALNVDLTEFQTNLVPYPRIHFPLTTYAPIISAEK 285          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub84D (gene:FBgn0003885 transcript:FBtr0081538)

HSP 1 Score: 235.728 bits (600), Expect = 1.016e-75
Identity = 126/251 (50.20%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.781 bits (164), Expect = 1.016e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub84B (gene:FBgn0003884 transcript:FBtr0081639)

HSP 1 Score: 235.343 bits (599), Expect = 1.428e-75
Identity = 125/251 (49.80%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.781 bits (164), Expect = 1.428e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub85E (gene:FBgn0003886 transcript:FBtr0082087)

HSP 1 Score: 230.72 bits (587), Expect = 2.587e-74
Identity = 123/251 (49.00%), Postives = 171/251 (68.13%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +HIGQ G+QIG A WEL   EHGI  DG M +    G  D+  S++T+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+ NLNRL+ Q+
Sbjct:    1 MRECISVHIGQAGVQIGNACWELYCLEHGIQPDGHMPSDKTVGGGDD--SFSTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDVVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYMNLNRLIGQI 234          

HSP 2 Score: 68.1662 bits (165), Expect = 2.587e-74
Identity = 27/48 (56.25%), Postives = 38/48 (79.17%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P + + K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISVEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: alphaTub67C (gene:FBgn0087040 transcript:FBtr0076393)

HSP 1 Score: 217.238 bits (552), Expect = 1.096e-69
Identity = 119/260 (45.77%), Postives = 165/260 (63.46%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLM----------NNGDVDEKASYN---TYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  + IGQCG+QIG A WEL + EHGI+ DG +          ++  V    S N   T+F  +  G+ VPRSIFVDLEPTV+D +R G  R+LYHP QLI+G EDAANN+ARG YSIGK+ + +V    +K AE CD++  FLI HS GGGTGSG                  +L+E LS +Y K+ K++  V+PSP +ST+VVEPYN LL  H+ M++SD     +NE+++ +C+++L  D   + NLNRL+AQ+
Sbjct:    1 MREVVSIQIGQCGIQIGNACWELYLLEHGINLDGSLKTKEELTASGSSASVGHDTSANDARTFFTETGNGKQVPRSIFVDLEPTVIDDVRNGCMRELYHPEQLISGKEDAANNYARGRYSIGKEVIDRVTSRLQKIAEQCDSLQGFLIFHSLGGGTGSG---------------FTSLLVERLSTDYSKKCKLDFAVYPSPKVSTAVVEPYNALLTTHSTMDHSDCVFMVDNEAIYDICNNSLGVDRPAYRNLNRLIAQI 245          

HSP 2 Score: 66.2402 bits (160), Expect = 1.096e-69
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S TTASLRF G MN ++ +FQTNLVP+PRIHFPL ++ P +   +
Sbjct:  244 QIVSSTTASLRFSGSMNVDLNEFQTNLVPFPRIHFPLVAYAPLMSAER 291          
BLAST of Tubulin alpha chain vs. Ensembl Fly
Match: CG7794 (gene:FBgn0038565 transcript:FBtr0083540)

HSP 1 Score: 170.244 bits (430), Expect = 4.588e-48
Identity = 96/247 (38.87%), Postives = 150/247 (60.73%), Query Frame = 2
Query:   65 DIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYV-PRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKR-NKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            +I  +HIGQ G+QI  A WEL   EHGI  +G +    +D+  S+ T+F  +     V PR + +D EPTV+D++RTG+YR L+HP+ LI G +D+ +NFARG+  +  + L + +   R+ A+ C N+  FL+  + GGG+GSG                   +ME L  ++GK+   +E  V+PSP++S  +VEPYN LLA H  M+ +D++   +NE+L+ +C + L+    T+TNLNR++AQV
Sbjct:    6 EIIQIHIGQAGVQIANACWELFCLEHGILANGRLTQSPMDD--SFLTFFEFTSHQPCVQPRLVMIDTEPTVIDEIRTGSYRNLFHPDTLITGKDDSGSNFARGYNLMASELLDRSMNAIRRVADRCRNLRGFLVFRAIGGGSGSGLGTR---------------IMERLVEDFGKKMTVVEFLVYPSPSISPVIVEPYNALLAAHFSMDCADVSFIVDNEALYDICANTLNVPAPTYTNLNRIIAQV 235          

HSP 2 Score: 51.2174 bits (121), Expect = 1.015e-6
Identity = 26/58 (44.83%), Postives = 35/58 (60.34%), Query Frame = 3
Query:  798 YASTQKIIVPDYQIQMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFV 971
            Y +  +II      Q++S  TAS RF G    +  + QTNLVPYPRIH+PL ++ P V
Sbjct:  225 YTNLNRIIA-----QVVSSFTASQRFGGGSTVSFQELQTNLVPYPRIHYPLINYAPLV 277          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba8l3 (tubulin, alpha 8 like 3 [Source:ZFIN;Acc:ZDB-GENE-040801-77])

HSP 1 Score: 237.654 bits (605), Expect = 4.847e-76
Identity = 126/251 (50.20%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   MH+GQ G Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR+IFVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISMHVGQAGAQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRKNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.847e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba8l4 (tubulin, alpha 8 like 4 [Source:ZFIN;Acc:ZDB-GENE-040426-860])

HSP 1 Score: 237.269 bits (604), Expect = 5.722e-76
Identity = 126/251 (50.20%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   MH+GQ G Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISMHVGQAGAQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.722e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: si:ch211-114n24.6 (si:ch211-114n24.6 [Source:ZFIN;Acc:ZDB-GENE-120214-26])

HSP 1 Score: 235.728 bits (600), Expect = 1.754e-75
Identity = 124/251 (49.40%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 1.754e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba1c (tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114-1])

HSP 1 Score: 234.572 bits (597), Expect = 4.125e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.125e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Match: tuba1c (tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114-1])

HSP 1 Score: 234.572 bits (597), Expect = 4.446e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.446e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: pex1 (peroxisomal biogenesis factor 1 [Source:Xenbase;Acc:XB-GENE-985415])

HSP 1 Score: 234.958 bits (598), Expect = 4.438e-75
Identity = 124/251 (49.40%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.438e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: pex1 (peroxisomal biogenesis factor 1 [Source:Xenbase;Acc:XB-GENE-985415])

HSP 1 Score: 234.958 bits (598), Expect = 4.863e-75
Identity = 124/251 (49.40%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.863e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 234.572 bits (597), Expect = 5.693e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.693e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 234.572 bits (597), Expect = 5.693e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.693e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Match: tuba1b (tubulin alpha 4a [Source:NCBI gene;Acc:100038108])

HSP 1 Score: 234.572 bits (597), Expect = 5.693e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.693e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1b (tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:107804])

HSP 1 Score: 234.572 bits (597), Expect = 3.865e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 3.865e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1c (tubulin, alpha 1C [Source:MGI Symbol;Acc:MGI:1095409])

HSP 1 Score: 234.572 bits (597), Expect = 3.865e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 3.865e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba3a (tubulin, alpha 3A [Source:MGI Symbol;Acc:MGI:1095406])

HSP 1 Score: 234.187 bits (596), Expect = 4.488e-75
Identity = 126/251 (50.20%), Postives = 174/251 (69.32%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF +               +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFAS---------------LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.488e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba3b (tubulin, alpha 3B [Source:MGI Symbol;Acc:MGI:1095408])

HSP 1 Score: 234.187 bits (596), Expect = 4.488e-75
Identity = 126/251 (50.20%), Postives = 174/251 (69.32%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSGF +               +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFAS---------------LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.488e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Mouse
Match: Tuba1a (tubulin, alpha 1A [Source:MGI Symbol;Acc:MGI:98869])

HSP 1 Score: 234.187 bits (596), Expect = 5.254e-75
Identity = 123/251 (49.00%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.254e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P30436|TBA_ONCKE (Tubulin alpha chain OS=Oncorhynchus keta OX=8018 PE=2 SV=2)

HSP 1 Score: 238.424 bits (607), Expect = 1.761e-75
Identity = 126/251 (50.20%), Postives = 174/251 (69.32%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  MH+GQ G+Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR+IFVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNRL+ Q+
Sbjct:    1 MREVISMHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTCGGGDD--SFNTFFSETGAGKHVPRAIFVDLEPTVIDEVRTGIYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDIVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 1.761e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P34690|TBA2_CAEEL (Tubulin alpha-2 chain OS=Caenorhabditis elegans OX=6239 GN=tba-2 PE=1 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 2.061e-75
Identity = 118/247 (47.77%), Postives = 171/247 (69.23%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  +H+GQ G+QIG A WEL   EHGI  DG M     +E  S+ T+F+ + +G+YVPRSIFVDLEPTVVD++RTG Y++L+HP Q+I G EDAANN+ARG Y++GK+ +  V+   R+ A+NC  +  F + HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  ++TNLNR+++QV
Sbjct:    1 MREVISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPTQSTNEGESFTTFFSDTGSGRYVPRSIFVDLEPTVVDEIRTGTYKKLFHPEQMITGKEDAANNYARGHYTVGKELIDTVLDRIRRLADNCSGLQGFFVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDVERPSYTNLNRIISQV 232          

HSP 2 Score: 66.2402 bits (160), Expect = 2.061e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  231 QVVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLAAYTPLISAEK 278          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P06605|TBA3_DROME (Tubulin alpha-3 chain OS=Drosophila melanogaster OX=7227 GN=alphaTub84D PE=2 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 7.714e-75
Identity = 126/251 (50.20%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.781 bits (164), Expect = 7.714e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P06603|TBA1_DROME (Tubulin alpha-1 chain OS=Drosophila melanogaster OX=7227 GN=alphaTub84B PE=1 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 1.084e-74
Identity = 125/251 (49.80%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.781 bits (164), Expect = 1.084e-74
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Match: sp|P18288|TBAT_ONCMY (Tubulin alpha chain, testis-specific OS=Oncorhynchus mykiss OX=8022 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.149e-74
Identity = 125/251 (49.80%), Postives = 174/251 (69.32%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK ++ C  +  FLI HSFGGGTGSGF +               +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRVRKLSDQCTGLQGFLIFHSFGGGTGSGFAS---------------LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 68.1662 bits (165), Expect = 1.149e-74
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A2D0PW74 (Tubulin alpha chain OS=Ictalurus punctatus OX=7998 GN=LOC108256914 PE=3 SV=1)

HSP 1 Score: 243.817 bits (621), Expect = 5.456e-75
Identity = 128/259 (49.42%), Postives = 179/259 (69.11%), Query Frame = 2
Query:   32 FKLIKSIAKMHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGD---VDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            F    S+ KM +   +HIGQ G+QIG + WEL   EHGI  DG +++G+   VD  +S+ T+F+ + AG+YVPR++F+DLEPTVVD++R G YRQLYHP QLI+G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD +   +NE++F +C  NLD D  +++NLNRL+AQ+
Sbjct:   15 FSFTSSLNKMRECISVHIGQAGVQIGNSCWELYCLEHGIQPDGTISSGNTCLVD--SSFGTFFSDTGAGKYVPRAVFIDLEPTVVDEIRNGHYRQLYHPEQLISGKEDAANNYARGHYTIGKEIVDSVLDRMRKMADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSVYPAPQVSTAVVEPYNSILTTHTTLEHSDCSFMVDNEAIFDICKRNLDIDRPSYSNLNRLIAQI 256          

HSP 2 Score: 68.1662 bits (165), Expect = 5.456e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  255 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEK 302          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A2D0PUJ4 (Tubulin alpha chain OS=Ictalurus punctatus OX=7998 GN=LOC108256914 PE=3 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 7.606e-75
Identity = 128/259 (49.42%), Postives = 179/259 (69.11%), Query Frame = 2
Query:   32 FKLIKSIAKMHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGD---VDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            F    S+ KM +   +HIGQ G+QIG + WEL   EHGI  DG +++G+   VD  +S+ T+F+ + AG+YVPR++F+DLEPTVVD++R G YRQLYHP QLI+G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD +   +NE++F +C  NLD D  +++NLNRL+AQ+
Sbjct:    6 FSFTSSLNKMRECISVHIGQAGVQIGNSCWELYCLEHGIQPDGTISSGNTCLVD--SSFGTFFSDTGAGKYVPRAVFIDLEPTVVDEIRNGHYRQLYHPEQLISGKEDAANNYARGHYTIGKEIVDSVLDRMRKMADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSVYPAPQVSTAVVEPYNSILTTHTTLEHSDCSFMVDNEAIFDICKRNLDIDRPSYSNLNRLIAQI 247          

HSP 2 Score: 68.1662 bits (165), Expect = 7.606e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  246 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEK 293          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A023FK91 (Tubulin alpha chain (Fragment) OS=Amblyomma cajennense OX=34607 PE=2 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.152e-74
Identity = 128/253 (50.59%), Postives = 177/253 (69.96%), Query Frame = 2
Query:   53 AKMHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            A M +   +HIGQ G+QIG A WEL   EHGI +DG M +    G +D+  S+NT+F  + AG++VPR++FVDLEPTVVD++R+GAYRQLYHP QLI+G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD D  T+TNLNRL++Q+
Sbjct:    8 AAMRECISVHIGQAGVQIGNACWELYCLEHGIQQDGQMPSDKVSGGIDD--SFNTFFAETGAGRHVPRAVFVDLEPTVVDEVRSGAYRQLYHPEQLISGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIDRPTYTNLNRLISQI 243          

HSP 2 Score: 67.781 bits (164), Expect = 1.152e-74
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  242 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 289          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: A0A131XG32 (Tubulin alpha chain (Fragment) OS=Hyalomma excavatum OX=257692 PE=2 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.283e-74
Identity = 127/258 (49.22%), Postives = 179/258 (69.38%), Query Frame = 2
Query:   38 LIKSIAKMHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            ++ +   M +   +HIGQ G+QIG A WEL   EHGI +DG M +    G +D+  S+NT+F  + AG++VPR++FVDLEPTVVD++R+GAYRQLYHP QLI+G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD D  T+TNLNRL++Q+
Sbjct:   17 VLPTTPNMRECISVHIGQAGVQIGNACWELYCLEHGIQQDGQMPSDKVSGGIDD--SFNTFFAETGAGRHVPRAVFVDLEPTVVDEVRSGAYRQLYHPEQLISGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIDRPTYTNLNRLISQI 257          

HSP 2 Score: 67.781 bits (164), Expect = 1.283e-74
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  256 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISSEK 303          
BLAST of Tubulin alpha chain vs. TrEMBL
Match: Q7YZZ3 (Tubulin alpha chain OS=Oikopleura dioica OX=34765 GN=TUBAD PE=3 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.394e-74
Identity = 126/251 (50.20%), Postives = 175/251 (69.72%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++ C+H+GQCG+Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPRS+FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P ++T+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MREVICIHVGQCGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRSVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQIATAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 1.394e-74
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000042562.1 (tubulin alpha chain [Source:NCBI gene;Acc:103038315])

HSP 1 Score: 238.039 bits (606), Expect = 3.454e-76
Identity = 126/251 (50.20%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   MH+GQ G Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR+IFVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISMHVGQAGAQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAIFVDLEPTVIDEIRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRKNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 3.454e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: si:ch211-114n24.6 (tubulin alpha-1A chain-like [Source:NCBI gene;Acc:103034569])

HSP 1 Score: 235.728 bits (600), Expect = 1.428e-75
Identity = 124/251 (49.40%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 1.428e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000030346.1 (tubulin alpha-1B chain [Source:NCBI gene;Acc:111195381])

HSP 1 Score: 234.572 bits (597), Expect = 3.587e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 3.587e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: ENSAMXT00000049713.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:103046666])

HSP 1 Score: 234.187 bits (596), Expect = 4.270e-75
Identity = 123/251 (49.00%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 4.270e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Match: tuba1a (tubulin alpha chain [Source:NCBI gene;Acc:103037973])

HSP 1 Score: 233.032 bits (593), Expect = 1.166e-74
Identity = 123/257 (47.86%), Postives = 174/257 (67.70%), Query Frame = 2
Query:   41 IKSIAKMHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            I + +   +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    6 ICNFSPQRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 245          

HSP 2 Score: 67.3958 bits (163), Expect = 1.166e-74
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  244 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 291          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 234.958 bits (598), Expect = 5.602e-76
Identity = 125/251 (49.80%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.602e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 166.007 bits (419), Expect = 2.294e-55
Identity = 98/252 (38.89%), Postives = 150/252 (59.52%), Query Frame = 2
Query:   62 HDIFCMHIGQCGLQIGFALWELLIAEHGISRDG-LMNNGDVDE--KASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQ---MRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
             D   +HIGQ G+Q+G A WEL   EHGI  DG L  + DV     +S+NT+F+ + +G++VPR+IF+DLEP+V+ +   +RT         + ++ G E  ++   RG    G+K   +V+   R  +E C  +  FL+ HSFGGGTGSG                  +LME LS +YGK++K+E  ++P+P +ST+VVEPYN +L  H  +E+SD +   +NE+++ +C   LD D  ++ NLNRL+AQ+
Sbjct:    3 RDCISIHIGQAGVQMGNACWELYCLEHGIKPDGHLATHPDVTSVVDSSFNTFFSETGSGKHVPRAIFIDLEPSVIGEPCGLRTLTVGGXLRMSMMLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSG---------------FTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQM 239          

HSP 2 Score: 67.3958 bits (163), Expect = 2.294e-55
Identity = 28/48 (58.33%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            QM+S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  238 QMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVISAEK 285          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005307.1 (pep scaffold:Pmarinus_7.0:GL485425:14485:17012:-1 gene:ENSPMAG00000004835.1 transcript:ENSPMAT00000005307.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 136.346 bits (342), Expect = 2.161e-39
Identity = 67/124 (54.03%), Postives = 88/124 (70.97%), Query Frame = 2
Query:   65 DIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRK 424
            +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVV  +RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK
Sbjct:    3 ECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSEKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVGAVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 124          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 133.65 bits (335), Expect = 2.975e-38
Identity = 85/228 (37.28%), Postives = 129/228 (56.58%), Query Frame = 2
Query:  116 LWELLIAEHGISRDGLMNNGDVD-EKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEY-LSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLA 793
             WE++  EHGI   G  + GD D +    N Y+N +  G+YVPR+I VDLEP  +D +R+G + Q++ P+  + G   A NN+A+G Y+ G + +  V+ + RK AE+CD +  F + HS GGGTGSG     I          +K+  EY + V Y     +  G  PSP +S +VVEPYN  L++H  +EN+D     +NE+L+ +C   L     T+ +LN L++
Sbjct:    2 FWEVISDEHGIDPTGSYH-GDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLI----------SKIREEYIIQVLY---TVVASG--PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVS 213          

HSP 2 Score: 42.743 bits (99), Expect = 2.975e-38
Identity = 18/41 (43.90%), Postives = 26/41 (63.41%), Query Frame = 3
Query:  846 ISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPF 968
            +SG T  LRF G +NA++     N+VP+PR+HF +  F P 
Sbjct:  216 MSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL 256          
BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 107.071 bits (266), Expect = 5.145e-29
Identity = 70/249 (28.11%), Postives = 124/249 (49.80%), Query Frame = 2
Query:   62 HDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYE--DAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSP-TLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
             +I  + +GQCG QIGF  W+ L AEHGIS DG++ +   +     + +F  +    Y+PR++ +DLEP V++ +   +Y  LY+P  +        A NN+A G +S G+K  +++  +  + A+  D++    +         +       K+    RF K K++  Y              VFP+   +S  VV+PYN+LL L    +N+D     +N +L R+  D L   + +F+ +N+L++ +
Sbjct:    3 REIITLQLGQCGNQIGFEFWKQLCAEHGISPDGILEDFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVINSIVNSSYANLYNPENIYLSEHGGGAGNNWASG-HSQGEKIHEEIFDIIDREADGSDSLEKCPLTRRLSATNWN----ELCKYFC--RFPK-KLIQTY-------------SVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTI 230          

HSP 2 Score: 37.7354 bits (86), Expect = 5.145e-29
Identity = 16/50 (32.00%), Postives = 29/50 (58.00%), Query Frame = 3
Query:  843 MISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDS 992
            ++S +T +LR+ G MN ++I    +L+P PR+HF +  + P      + S
Sbjct:  230 IMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVAS 279          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 214.542 bits (545), Expect = 6.348e-70
Identity = 112/250 (44.80%), Postives = 163/250 (65.20%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKAS---YNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  +++GQ G QIG A WEL   EHGI  DG + +G    K     ++T+F+ +  G++VPR+I+VDLEP V+D++RTG +++L+HP QLING EDAANN+ARG Y++G++ + +V    RK A+ CD +  FL  HS GGGTGSG  +                 +E LS EYGK++K+E  V+P+P LSTSVVEPYNT+L  H  +E++D     +NE+++ +C  NL     +F+NLN L+AQV
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLL---------------LENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQV 235          

HSP 2 Score: 66.6254 bits (161), Expect = 6.348e-70
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSAS 998
            Q+IS  TASLRF+G +N ++ +FQTNLVPYPRIHFPL S+ P +   +    S
Sbjct:  234 QVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHES 286          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 207.608 bits (527), Expect = 6.500e-68
Identity = 112/250 (44.80%), Postives = 161/250 (64.40%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKAS---YNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M ++  +++GQ G QIG A WEL   EHGI  DG + +G    K     ++T+F+ +  G++VPR+I+VDLEP V+D++R G Y+ L+HP QLI+G EDAANN+ARG Y++G++ L  V+   RK A+ CD +  FL  HS GGGTGSG  +                 +E LS EYGK++K+E  V+P+P +STSVVEPYNT+L  H  +E++D     +NE+++ +C  NLD    +F NLN L+AQV
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLL---------------LEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQV 235          

HSP 2 Score: 67.0106 bits (162), Expect = 6.500e-68
Identity = 28/48 (58.33%), Postives = 38/48 (79.17%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL S+ P +  +K
Sbjct:  234 QVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSK 281          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 155.221 bits (391), Expect = 2.505e-45
Identity = 92/247 (37.25%), Postives = 133/247 (53.85%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +I  +  GQCG QIG A WE +  EHG+  +G  +  D  +K   N YFN + +G++VPRSI VDLEP  +D +R  A   L+ P+  I G   A N +A+G Y+ G + +  V+ + R+ AE CD++  F I HS GGGTGSG     I  I                 E+  R      V PSP  S +VVEPYN  L++H  +E+SD     +NE+L+ +C   L  +  ++ +LN L++ V
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIRE---------------EFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSV 232          

HSP 2 Score: 43.8986 bits (102), Expect = 2.505e-45
Identity = 17/45 (37.78%), Postives = 29/45 (64.44%), Query Frame = 3
Query:  843 MISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGM 977
            ++SG T SLR+ G +N+++     NLVP+PR+HF +  + P   +
Sbjct:  232 VMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAI 276          
BLAST of Tubulin alpha chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 88.1965 bits (217), Expect = 5.079e-20
Identity = 74/255 (29.02%), Postives = 119/255 (46.67%), Query Frame = 2
Query:   65 DIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNT--YFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQ--LINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQVCFNSKN 817
            +I  +  GQCG  +G  LW  L  EH I  DGL    D   +   +T  +F  +   ++ PR+I +D EP+V+  +    +R  + P    + +    A N++A G Y IG +    ++    K  ++ DN   F + HS  GGTGSG  ++               L+E L   Y K+      VFP+ + S  VV+ YNT+LAL   +E+SD    F+N SL  +        +I   + N+L++ +  +  N
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVEN-TFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSN---------------LLEALCDRYPKKILTTYSVFPARS-SEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTN 240          

HSP 2 Score: 25.7942 bits (55), Expect = 5.079e-20
Identity = 16/43 (37.21%), Postives = 24/43 (55.81%), Query Frame = 3
Query:  843 MISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFV 971
            +IS  T S+RF   M +++    + L+P P +HF   SF PF 
Sbjct:  234 IISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFT 276          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO46332 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQB6])

HSP 1 Score: 233.802 bits (595), Expect = 1.536e-76
Identity = 118/249 (47.39%), Postives = 172/249 (69.08%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKA--SYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    ++   S++T+F+ + +G++VPR++FVDLEPTV+D++RTG YRQL+HP Q++ G EDAANN+ARG Y++GK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                + +LME LSV+YGKR+K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECLSIHVGQAGVQIGNACWELYCLEHGIQPDGHMPSDQSADRCDDSFSTFFSETASGKHVPRAVFVDLEPTVIDEIRTGTYRQLFHPEQMVTGKEDAANNYARGHYTVGKELIDVVLDKIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FSSLLMERLSVDYGKRSKLEFAVYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 71.2478 bits (173), Expect = 1.536e-76
Identity = 28/48 (58.33%), Postives = 39/48 (81.25%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +G +K
Sbjct:  233 QIVSSVTASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVIGADK 280          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO38066 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDY2])

HSP 1 Score: 235.343 bits (599), Expect = 6.195e-76
Identity = 125/251 (49.80%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                + +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FSSLLMERLSVDYGKKSKLEFAIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLMGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 6.195e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO38067 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDY3])

HSP 1 Score: 235.343 bits (599), Expect = 6.844e-76
Identity = 124/251 (49.40%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y++GK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                + +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKELIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FSSLLMERLSVDYGKKSKLEFAIYPAPQISTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 6.844e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO44803 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT0])

HSP 1 Score: 234.958 bits (598), Expect = 7.687e-76
Identity = 124/251 (49.40%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y++GK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 7.687e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Match: EDO38091 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDY0])

HSP 1 Score: 234.958 bits (598), Expect = 8.852e-76
Identity = 124/251 (49.40%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTVVD++RTG YRQL+HP QLI G EDAANN+ARG Y++GK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                + +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKELIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FSSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 8.852e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: tuba8l3 (tubulin alpha chain [Source:NCBI gene;Acc:101168255])

HSP 1 Score: 238.039 bits (606), Expect = 3.377e-76
Identity = 127/251 (50.60%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   MH+GQ G Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR+IFVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISMHVGQAGAQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 3.377e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000003572.2 (tubulin alpha chain [Source:NCBI gene;Acc:101171518])

HSP 1 Score: 237.269 bits (604), Expect = 5.604e-76
Identity = 126/251 (50.20%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   MH+GQ G Q+G A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR+IFVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y++GK+ +  V+  TRK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL+ Q+
Sbjct:    1 MRECISMHVGQAGAQMGNACWELYCLEHGIQPDGRMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.604e-76
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000020468.2 (tubulin alpha-1A chain-like [Source:NCBI gene;Acc:101155224])

HSP 1 Score: 234.958 bits (598), Expect = 1.775e-75
Identity = 124/251 (49.40%), Postives = 170/251 (67.73%), Query Frame = 2
Query:   53 AKMHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKA--SYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            A M +   +HIGQ G+Q+G A WEL   EHGI  DG M +         S+NT+F+ + AG++VPR++FVDLEP+VVD++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FLI HSFGGGTGSG                  +LME LSV+YGK++K+E  V+P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ LC  NLD +  ++TNLNRL+ Q+
Sbjct:   41 AIMRECISIHIGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTISGGDDSFNTFFSETGAGKHVPRAVFVDLEPSVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDLCRRNLDIERPSYTNLNRLIGQI 276          

HSP 2 Score: 68.1662 bits (165), Expect = 1.775e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  275 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 322          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: tuba1c (tubulin alpha-1B chain-like [Source:NCBI gene;Acc:101167538])

HSP 1 Score: 234.572 bits (597), Expect = 3.687e-75
Identity = 123/251 (49.00%), Postives = 173/251 (68.92%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN++L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTLGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 3.687e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Ensembl Medaka
Match: ENSORLT00000033163.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 233.802 bits (595), Expect = 5.139e-75
Identity = 123/251 (49.00%), Postives = 172/251 (68.53%), Query Frame = 2
Query:   59 MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNN----GDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            M +   +H+GQ G+QIG A WEL   EHGI  DG M +    G  D+  S+NT+F+ + AG++VPR++FVDLEPTV+D++RTG YRQL+HP QLI G EDAANN+ARG Y+IGK+ +  V+   RK A+ C  +  FL+ HSFGGGTGSG                  +LME LSV+YGK++K+E  ++P+P +ST+VVEPYN +L  H  +E+SD A   +NE+++ +C  NLD +  T+TNLNRL++Q+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDD--SFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSG---------------FTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQI 234          

HSP 2 Score: 67.3958 bits (163), Expect = 5.139e-75
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNK 983
            Q++S  TASLRF+G +N ++ +FQTNLVPYPRIHFPL ++ P +   K
Sbjct:  233 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK 280          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000017516.1 (SMESG000017516.1)

HSP 1 Score: 301.597 bits (771), Expect = 1.703e-109
Identity = 157/202 (77.72%), Postives = 174/202 (86.14%), Query Frame = 2
Query:  194 SYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            ++  +F  +  G++ PRS+ VDLEPTV+D+++ G YR L++P+ LIN YEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTA               +LMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFD+ITFTNLNRLLAQ+
Sbjct:    2 TFENFFLQTGNGKFTPRSLLVDLEPTVIDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTA---------------MLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQM 188          

HSP 2 Score: 114.39 bits (285), Expect = 1.703e-109
Identity = 56/68 (82.35%), Postives = 59/68 (86.76%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSAS**NFITKDNFRNLFV 1043
            QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSA   N  TK+    +F+
Sbjct:  187 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSA---NLTTKEISEQVFL 251          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000017516.1 (SMESG000017516.1)

HSP 1 Score: 300.056 bits (767), Expect = 6.950e-109
Identity = 157/202 (77.72%), Postives = 174/202 (86.14%), Query Frame = 2
Query:  194 SYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQV 799
            ++  +F  +  G++ PRS+ VDLEPTV+D+++ G YR L++P+ LIN YEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTA               +LMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFD+ITFTNLNRLLAQ+
Sbjct:    2 TFENFFLQTGNGKFTPRSLLVDLEPTVIDEIKLGKYRNLFNPDSLINSYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTA---------------MLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDEITFTNLNRLLAQM 188          

HSP 2 Score: 114.005 bits (284), Expect = 6.950e-109
Identity = 56/68 (82.35%), Postives = 59/68 (86.76%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSAS**NFITKDNFRNLFV 1043
            QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSA   N  TK+    +F+
Sbjct:  187 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKIDSA---NLTTKEISEQVFL 251          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000002455.1 (SMESG000002455.1)

HSP 1 Score: 290.041 bits (741), Expect = 1.221e-90
Identity = 157/259 (60.62%), Postives = 187/259 (72.20%), Query Frame = 2
Query:   62 HDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQVCFNSKNHCSRLSN 838
            HDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGF+++G+   +      RK  E  D+I    I  S GGGTGSG TA+  + +L                EY K +K+E  + PSP LS ++VEPYN +L+ H  M++ D++   +NESL+ +  + +   + T  ++NRL+AQVC +S    SR  N
Sbjct:   98 HDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFHTVGRIMKRVCENRIRKEFEINDSIQGIHIYRSLGGGTGSGMTATLFESLL----------------EYPKISKVEIPIHPSPHLSCAIVEPYNCILSEHFCMDDVDISVLIDNESLYDIMLEQIGLKEPTLHHINRLVAQVC-SSLTASSRFEN 339          

HSP 2 Score: 50.447 bits (119), Expect = 2.032e-6
Identity = 26/49 (53.06%), Postives = 31/49 (63.27%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKI 986
            Q+ S  TAS RFE  +  N     TNLVPYPRIHFPL S+ PF   +K+
Sbjct:  326 QVCSSLTASSRFENEL-YNDFSVLTNLVPYPRIHFPLVSYAPFGSESKL 373          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000002455.1 (SMESG000002455.1)

HSP 1 Score: 277.715 bits (709), Expect = 5.372e-87
Identity = 152/254 (59.84%), Postives = 182/254 (71.65%), Query Frame = 2
Query:   77 MHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESLFRLCHDNLDFDDITFTNLNRLLAQVCFNSKNHCSRLSN 838
            MHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGF+++G+   +      RK  E  D+I    I  S GGGTGSG TA+  + +L                EY K +K+E  + PSP LS ++VEPYN +L+ H  M++ D++   +NESL+ +  + +   + T  ++NRL+AQVC +S    SR  N
Sbjct:    1 MHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFHTVGRIMKRVCENRIRKEFEINDSIQGIHIYRSLGGGTGSGMTATLFESLL----------------EYPKISKVEIPIHPSPHLSCAIVEPYNCILSEHFCMDDVDISVLIDNESLYDIMLEQIGLKEPTLHHINRLVAQVC-SSLTASSRFEN 237          

HSP 2 Score: 50.447 bits (119), Expect = 2.007e-6
Identity = 26/49 (53.06%), Postives = 31/49 (63.27%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKI 986
            Q+ S  TAS RFE  +  N     TNLVPYPRIHFPL S+ PF   +K+
Sbjct:  224 QVCSSLTASSRFENEL-YNDFSVLTNLVPYPRIHFPLVSYAPFGSESKL 271          
BLAST of Tubulin alpha chain vs. Planmine SMEST
Match: SMESG000037910.1 (SMESG000037910.1)

HSP 1 Score: 233.802 bits (595), Expect = 1.636e-79
Identity = 122/219 (55.71%), Postives = 155/219 (70.78%), Query Frame = 2
Query:   68 IFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTYFNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANNFARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHXXXXXXXXXXXASTIKFILH*RFDKNKVLMEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAFFENESL 724
            I    IGQCG QI  A WEL   EH IS DG   +  V + +S+  +F+ S +GQ+VPRS+F+DLEPTV+D+++ G YRQL+HP  LI+  EDA+NNF+RGFYS GK A+++V+  TRK  ENCD + AF+ +HSFGGGTGSG                  +L+EYL+VEY KR K+E GVFPSPTLS +VVEPYN +LALH PMEN+D+A FFENESL
Sbjct:    5 IITFFIGQCGCQIASASWELYCLEHSISPDGRFYDDSVPDDSSFYNFFSESRSGQFVPRSLFIDLEPTVLDEIKVGRYRQLFHPENLISRNEDASNNFSRGFYSCGKLAIKEVIRNTRKLVENCDEVQAFMAHHSFGGGTGSG---------------FTSMLLEYLAVEYSKRTKVEFGVFPSPTLSVAVVEPYNAVLALHTPMENADVAVFFENESL 208          

HSP 2 Score: 82.0333 bits (201), Expect = 1.636e-79
Identity = 35/49 (71.43%), Postives = 42/49 (85.71%), Query Frame = 3
Query:  840 QMISGTTASLRFEGPMNANIIDFQTNLVPYPRIHFPLFSFYPFVGMNKI 986
            Q+ISG T S+RFEGP+N NIID+QTNLVPYPRIHFPL SF PF+  +K+
Sbjct:  212 QVISGMTCSMRFEGPINTNIIDYQTNLVPYPRIHFPLVSFSPFLAQSKL 260          
The following BLAST results are available for this feature:
BLAST of Tubulin alpha chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBA3C4.526e-7550.20tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
TUBA1C4.998e-7549.00tubulin alpha 1c [Source:HGNC Symbol;Acc:HGNC:2076... [more]
TUBA1B5.297e-7549.00tubulin alpha 1b [Source:HGNC Symbol;Acc:HGNC:1880... [more]
TUBA3C6.150e-7550.20tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
TUBA3D6.150e-7550.20tubulin alpha 3d [Source:HGNC Symbol;Acc:HGNC:2407... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tba-22.004e-7647.77Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Pro... [more]
tba-22.004e-7647.77Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Pro... [more]
tba-22.004e-7647.77Tubulin alpha-2 chain [Source:UniProtKB/Swiss-Pro... [more]
tba-47.561e-7647.77Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:... [more]
tba-92.669e-7549.00Tubulin alpha chain [Source:UniProtKB/TrEMBL;Acc:... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alphaTub84D1.016e-7550.20gene:FBgn0003885 transcript:FBtr0081538[more]
alphaTub84B1.428e-7549.80gene:FBgn0003884 transcript:FBtr0081639[more]
alphaTub85E2.587e-7449.00gene:FBgn0003886 transcript:FBtr0082087[more]
alphaTub67C1.096e-6945.77gene:FBgn0087040 transcript:FBtr0076393[more]
CG77944.588e-4838.87gene:FBgn0038565 transcript:FBtr0083540[more]
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BLAST of Tubulin alpha chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tuba8l34.847e-7650.20tubulin, alpha 8 like 3 [Source:ZFIN;Acc:ZDB-GENE-... [more]
tuba8l45.722e-7650.20tubulin, alpha 8 like 4 [Source:ZFIN;Acc:ZDB-GENE-... [more]
si:ch211-114n24.61.754e-7549.40si:ch211-114n24.6 [Source:ZFIN;Acc:ZDB-GENE-120214... [more]
tuba1c4.125e-7549.00tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114... [more]
tuba1c4.446e-7549.00tubulin, alpha 1c [Source:ZFIN;Acc:ZDB-GENE-061114... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pex14.438e-7549.40peroxisomal biogenesis factor 1 [Source:Xenbase;Ac... [more]
pex14.863e-7549.40peroxisomal biogenesis factor 1 [Source:Xenbase;Ac... [more]
tuba1b5.693e-7549.00tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
tuba1b5.693e-7549.00tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
tuba1b5.693e-7549.00tubulin alpha 4a [Source:NCBI gene;Acc:100038108][more]
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BLAST of Tubulin alpha chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tuba1b3.865e-7549.00tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:10780... [more]
Tuba1c3.865e-7549.00tubulin, alpha 1C [Source:MGI Symbol;Acc:MGI:10954... [more]
Tuba3a4.488e-7550.20tubulin, alpha 3A [Source:MGI Symbol;Acc:MGI:10954... [more]
Tuba3b4.488e-7550.20tubulin, alpha 3B [Source:MGI Symbol;Acc:MGI:10954... [more]
Tuba1a5.254e-7549.00tubulin, alpha 1A [Source:MGI Symbol;Acc:MGI:98869... [more]
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BLAST of Tubulin alpha chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P30436|TBA_ONCKE1.761e-7550.20Tubulin alpha chain OS=Oncorhynchus keta OX=8018 P... [more]
sp|P34690|TBA2_CAEEL2.061e-7547.77Tubulin alpha-2 chain OS=Caenorhabditis elegans OX... [more]
sp|P06605|TBA3_DROME7.714e-7550.20Tubulin alpha-3 chain OS=Drosophila melanogaster O... [more]
sp|P06603|TBA1_DROME1.084e-7449.80Tubulin alpha-1 chain OS=Drosophila melanogaster O... [more]
sp|P18288|TBAT_ONCMY1.149e-7449.80Tubulin alpha chain, testis-specific OS=Oncorhynch... [more]
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BLAST of Tubulin alpha chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2D0PW745.456e-7549.42Tubulin alpha chain OS=Ictalurus punctatus OX=7998... [more]
A0A2D0PUJ47.606e-7549.42Tubulin alpha chain OS=Ictalurus punctatus OX=7998... [more]
A0A023FK911.152e-7450.59Tubulin alpha chain (Fragment) OS=Amblyomma cajenn... [more]
A0A131XG321.283e-7449.22Tubulin alpha chain (Fragment) OS=Hyalomma excavat... [more]
Q7YZZ31.394e-7450.20Tubulin alpha chain OS=Oikopleura dioica OX=34765 ... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000042562.13.454e-7650.20tubulin alpha chain [Source:NCBI gene;Acc:10303831... [more]
si:ch211-114n24.61.428e-7549.40tubulin alpha-1A chain-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000030346.13.587e-7549.00tubulin alpha-1B chain [Source:NCBI gene;Acc:11119... [more]
ENSAMXT00000049713.14.270e-7549.00tubulin alpha-1A chain [Source:NCBI gene;Acc:10304... [more]
tuba1a1.166e-7447.86tubulin alpha chain [Source:NCBI gene;Acc:10303797... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBA3C5.602e-7649.80tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
ENSPMAT00000001139.12.294e-5538.89pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
ENSPMAT00000005307.12.161e-3954.03pep scaffold:Pmarinus_7.0:GL485425:14485:17012:-1 ... [more]
tubb22.975e-3837.28tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
tubg15.145e-2928.11tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB36.348e-7044.80Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB16.500e-6844.80Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB22.505e-4537.25Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB45.079e-2029.02Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO463321.536e-7647.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380666.195e-7649.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380676.844e-7649.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448037.687e-7649.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380918.852e-7649.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin alpha chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tuba8l33.377e-7650.60tubulin alpha chain [Source:NCBI gene;Acc:10116825... [more]
ENSORLT00000003572.25.604e-7650.20tubulin alpha chain [Source:NCBI gene;Acc:10117151... [more]
ENSORLT00000020468.21.775e-7549.40tubulin alpha-1A chain-like [Source:NCBI gene;Acc:... [more]
tuba1c3.687e-7549.00tubulin alpha-1B chain-like [Source:NCBI gene;Acc:... [more]
ENSORLT00000033163.15.139e-7549.00tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
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BLAST of Tubulin alpha chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017516.11.703e-10977.72SMESG000017516.1[more]
SMESG000017516.16.950e-10977.72SMESG000017516.1[more]
SMESG000002455.11.221e-9060.62SMESG000002455.1[more]
SMESG000002455.15.372e-8759.84SMESG000002455.1[more]
SMESG000037910.11.636e-7955.71SMESG000037910.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30021022 ID=SMED30021022|Name=Tubulin alpha chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1214bp
CCAATATGGATTTAATGTTGAGTATTCATAATTTAAATTAATAAAATCAA
TTGCCAAAATGCACGACATATTTTGCATGCATATTGGACAGTGCGGTTTA
CAGATTGGGTTTGCCCTGTGGGAGCTGCTTATAGCAGAACATGGCATATC
GAGAGACGGTCTAATGAATAATGGAGACGTAGACGAAAAAGCATCTTACA
ATACTTATTTCAATGTATCTATGGCTGGTCAATATGTTCCTCGTAGCATC
TTTGTAGATCTTGAACCGACAGTTGTTGACCAAATGAGAACTGGAGCTTA
CCGTCAGTTATATCATCCGAATCAGCTGATTAATGGATATGAAGATGCAG
CAAACAATTTTGCTAGAGGATTTTACTCTATTGGTAAAAAGGCATTACAA
AAAGTGGTTTATATGACTCGCAAAACTGCTGAAAACTGTGATAATATTCA
TGCATTTCTGATAAATCATTCATTCGGTGGTGGTACCGGTTCTGGCTTCA
CTGCAAGTACAATAAAATTTATTTTACATTAAAGATTTGACAAAAATAAA
GTGTTAATGGAATATCTATCTGTGGAGTATGGAAAAAGGAATAAAATTGA
ATGTGGAGTATTCCCAAGCCCTACACTTAGTACTTCAGTGGTGGAGCCGT
ACAATACCTTATTAGCATTGCATGCACCAATGGAAAATTCAGATCTCGCC
GCTTTTTTTGAAAATGAATCATTGTTTCGACTCTGCCACGATAATTTAGA
CTTCGATGACATCACTTTCACAAATTTAAATCGCCTTCTCGCACAAGTAT
GCTTCAACTCAAAAAATCATTGTTCCCGATTATCAAATTCAGATGATAAG
TGGGACGACTGCATCACTAAGATTTGAAGGTCCTATGAATGCAAACATAA
TCGACTTCCAAACAAATCTGGTACCATATCCAAGAATACATTTCCCATTA
TTCAGTTTTTATCCATTCGTAGGAATGAATAAAATCGACAGTGCTAGTTA
GTAAAATTTTATTACTAAAGACAATTTCCGAAACTTATTTGTTATTTTGA
GCTTTTTAAACTATAATGTTTTGGTAAAAGAAATTTAAGCTGCAAGTCAC
GGCTTAGTTCAGTAGTTGATAATTTCATTCTGGGCTCAGGTAATGATGTA
ATGTCATGTGGGGATTTTTTGTGGCTAATCTTATAATGAATTTTACAGTG
CTATTAGTACCACC
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protein sequence of SMED30021022-orf-1

>SMED30021022-orf-1 ID=SMED30021022-orf-1|Name=SMED30021022-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=158bp
MHDIFCMHIGQCGLQIGFALWELLIAEHGISRDGLMNNGDVDEKASYNTY
FNVSMAGQYVPRSIFVDLEPTVVDQMRTGAYRQLYHPNQLINGYEDAANN
FARGFYSIGKKALQKVVYMTRKTAENCDNIHAFLINHSFGGGTGSGFTAS
TIKFILH*
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protein sequence of SMED30021022-orf-2

>SMED30021022-orf-2 ID=SMED30021022-orf-2|Name=SMED30021022-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
MEYLSVEYGKRNKIECGVFPSPTLSTSVVEPYNTLLALHAPMENSDLAAF
FENESLFRLCHDNLDFDDITFTNLNRLLAQVCFNSKNHCSRLSNSDDKWD
DCITKI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007010cytoskeleton organization
GO:0007017microtubule-based process
GO:0000132establishment of mitotic spindle orientation
GO:0008104protein localization
GO:0009792embryo development ending in birth or egg hatching
GO:0032465regulation of cytokinesis
GO:0051642centrosome localization
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR003008Tubulin_FtsZ_GTPase
IPR008280Tub_FtsZ_C
IPR000217Tubulin
IPR002452Alpha_tubulin
IPR017975Tubulin_CS
Vocabulary: molecular function
TermDefinition
GO:0003924GTPase activity
GO:0005200structural constituent of cytoskeleton
GO:0005525GTP binding
GO:0000166nucleotide binding
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton