G2/mitotic-specific cyclin-B3

Overview


Name	G2/mitotic-specific cyclin-B3
Smed ID	SMED30021004
Length (bp)	2322

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of G2/mitotic-specific cyclin-B3 (SMED30021004) t-SNE clustered cells

Violin plots show distribution of expression levels for G2/mitotic-specific cyclin-B3 (SMED30021004) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G2/mitotic-specific cyclin-B3 (SMED30021004) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G2/mitotic-specific cyclin-B3 (SMED30021004) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30021004

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
X1 cell	SMED30021004	SMESG000001506.1	SmedASXL_015418	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015

Note: Hover over icons to view figure legend

Homology

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BLAST of G2/mitotic-specific cyclin-B3 vs. UniProt/SwissProt
Match: sp|P36630|CG22_SCHPO (G2/mitotic-specific cyclin cig2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cig2 PE=1 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 2.197e-8
Identity = 60/201 (29.85%), Postives = 102/201 (50.75%), Query Frame = 1

Query:  937 HISS--LMASNINTSHFHDLHLEELQCNKQFDFMRNSTQLT---GSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEGLNIISNIKDF---ADCKHT--DLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPESKRAAAAMWLAR 1509
            H+SS  LMA       F  +   +L+C     +M    +LT     IL  ++ +I   SN   +P + ++ AV++I  +LSR     L   +   + A+ +A+K E + +  +I++F    D   T  D+  AE  ++NV +F+L+ P  +NF  +    E  ++ T  L  ++ E+ L  + LL  P SK AAAAM+L+R
Sbjct:  125 HLSSNPLMAEEYAPEIFEYIRKLDLKCLPNPKYMDQQKELTWKMREILNEWLVEIH--SNFCLMP-ETLYLAVNIIDRFLSR-RSCSLSKFQLTGITALLIASKYEEV-MCPSIQNFVYMTDGAFTVEDVCVAERYMLNVLNFDLSYPSPLNFLRKISQAEGYDAQTRTLGKYLTEIYLFDHDLLRYPMSKIAAAAMYLSR 320

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BLAST of G2/mitotic-specific cyclin-B3 vs. UniProt/SwissProt
Match: sp|P30284|CG21_EMENI (G2/mitotic-specific cyclin-B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=nimE PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 6.838e-8
Identity = 51/193 (26.42%), Postives = 97/193 (50.26%), Query Frame = 1

Query:  949 LMASNINTSHFHDLHLEELQCNKQFDFMRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEGL--NIISNIKDFADCKHTDLQ--QAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPESKRAAAAMWLARLI 1515
            LMA+      F  L   E++     D++ +   L   + G  ++ + E+     L  + +  AV++I  +LS    V L  ++ V + A+++A+K E +    ++N    AD   +D +   AE  ++   ++N++ P+ +NF  R    +  +  T  L  +++E+SL  +  L  P+S+  AAAM+LARLI
Sbjct:  203 LMAAEYVVEIFDYLRELEMETLPNPDYIDHQPDLEWKMRGILVDWLIEVHTRFRLLPETLFLAVNIIDRFLS-AEVVALDRLQLVGVAAMFIASKYEEVLSPHVANFSHVADETFSDKEILDAERHILATLEYNMSYPNPMNFLRRISKADNYDIQTRTLGKYLMEISLLDHRFLGYPQSQIGAAAMYLARLI 394

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BLAST of G2/mitotic-specific cyclin-B3 vs. UniProt/SwissProt
Match: sp|P10815|CG23_SCHPO (G2/mitotic-specific cyclin cdc13 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cdc13 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.528e-7
Identity = 52/193 (26.94%), Postives = 100/193 (51.81%), Query Frame = 1

Query:  949 LMASNINTSHFHDLHLEELQCNKQFDFMRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLS-RVHDVPLVDMRWVTLGAVWVAAKNEGLNIISNIKDF---ADCKHTDLQ--QAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPESKRAAAAMWLAR 1509
            LM S      F  L+  E++      +M    +L   + G   + + E+ +   L  + +  AV++I  +LS RV    L  ++ V + A+++A+K E + +  ++++F   AD  + + +  QAE  ++ V +FNL  P+ +NF  R    +  +  T  ++ +++E+ L  + LL  P S++ AAAM+LAR
Sbjct:  198 LMVSEYVVDIFEYLNELEIETMPSPTYMDRQKELAWKMRGILTDWLIEVHSRFRLLPETLFLAVNIIDRFLSLRV--CSLNKLQLVGIAALFIASKYEEV-MCPSVQNFVYMADGGYDEEEILQAERYILRVLEFNLAYPNPMNFLRRISKADFYDIQTRTVAKYLVEIGLLDHKLLPYPPSQQCAAAMYLAR 387

[Close]

BLAST of G2/mitotic-specific cyclin-B3 vs. UniProt/SwissProt
Match: sp|Q7XSJ6|CCB21_ORYSJ (Cyclin-B2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCB2-1 PE=1 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 9.054e-6
Identity = 46/175 (26.29%), Postives = 87/175 (49.71%), Query Frame = 1

Query:  997 EELQCNKQFDFMRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNE--GLNIISNIKDFADCKHTDLQQAELR--LINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPESKRAAAAMWLAR 1509
            EE+ C  Q D+M +   +   +    I+ + E+ +   L ++ +   V+++  +L +   VP   ++ V + A+ +A K E   + ++ ++   +D  +T  Q  E+   ++N   FN++ P    F  RFL    ++     LS FILE+SL  Y +L    S  AAAA++ A+
Sbjct:  166 EEMSC-VQPDYMSSQGDINEKMRAILIDWLIEVHHKFELMDETLFLTVNIVDRFLEK-QVVPRKKLQLVGVTAMLLACKYEEVAVPVVEDLVLISDRAYTKGQILEMEKLILNTLQFNMSVPTPYVFMRRFLKAAQSDKQLQLLSFFILELSLVEYQMLKYRPSLLAAAAVYTAQ 338

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BLAST of G2/mitotic-specific cyclin-B3 vs. TrEMBL
Match: A0A4Z2CRG4 (G2/mitotic-specific cyclin-B3 OS=Schistosoma japonicum OX=6182 GN=EWB00_008109 PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.785e-11
Identity = 53/172 (30.81%), Postives = 91/172 (52.91%), Query Frame = 1

Query: 1024 DFMRNSTQ--LTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEG-----LNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSS-FILEMSLHSYTLLLEPESKRAAAAMWLARLI 1515
            DF+    Q  L+  +L    + + E+     L ++ IH A  L+Y +L R   +   +++ +   A+ VA K+E      LN    I D A  K  +  +AE RL+N  DF + +P+   F  R+  +  A+S  +  +S F+LE  +HS+ + L+ ES++AAA +WLAR++
Sbjct:  240 DFLAAGKQPNLSADMLTTLADWLVEVQENFALNHETIHLAWGLLYAFLDRGQPLARREIQLMACAAIMVACKHEERQMSHLNEFLYITDNAYTKE-EFIEAERRLLNAIDFAVHRPNPYVFLRRYARVLDAHSGPVQFTSRFLLEAGMHSHAISLKRESRKAAAVLWLARVV 410

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BLAST of G2/mitotic-specific cyclin-B3 vs. TrEMBL
Match: A0A3P8BQ90 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS16081 PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.433e-10
Identity = 56/181 (30.94%), Postives = 94/181 (51.93%), Query Frame = 1

Query:  997 EELQCNKQFDFMRNSTQ--LTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEG-----LNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSS-FILEMSLHSYTLLLEPESKRAAAAMWLARLI 1515
            E+ Q N   DF+    Q  L   +L    + + E+     L ++ IH A  L+Y +L R   +   +++ +   A+ VA K+E      LN    I D A  K  +  +AE RL+   DF + +P+   F  R+  +  A+S  +  +S F+LE  +HS+T+ L+ ES++AAA +WLAR++
Sbjct:  162 EDFQVN---DFLAAGKQPNLNADMLTTLADWLVEVQENFALNHETIHLAWGLLYAFLDRGQPLARREIQLMACAAIMVACKHEERQMPHLNEFLYITDNAYTKE-EFIEAERRLLIAIDFAVHRPNPYVFLRRYARVLDAHSGPVQFTSRFLLEAGMHSHTISLKRESRKAAAVLWLARVV 338

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BLAST of G2/mitotic-specific cyclin-B3 vs. TrEMBL
Match: A0A183MJ56 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.485e-10
Identity = 56/181 (30.94%), Postives = 94/181 (51.93%), Query Frame = 1

Query:  997 EELQCNKQFDFMRNSTQ--LTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEG-----LNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSS-FILEMSLHSYTLLLEPESKRAAAAMWLARLI 1515
            E+ Q N   DF+    Q  L   +L    + + E+     L ++ IH A  L+Y +L R   +   +++ +   A+ VA K+E      LN    I D A  K  +  +AE RL+   DF + +P+   F  R+  +  A+S  +  +S F+LE  +HS+T+ L+ ES++AAA +WLAR++
Sbjct:  162 EDFQVN---DFLAAGKQPNLNADMLTTLADWLVEVQENFALNHETIHLAWGLLYAFLDRGQPLARREIQLMACAAIMVACKHEERQMPHLNEFLYITDNAYTKE-EFIEAERRLLIAIDFAVHRPNPYVFLRRYARVLDAHSGPVQFTSRFLLEAGMHSHTISLKRESRKAAAVLWLARVV 338

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BLAST of G2/mitotic-specific cyclin-B3 vs. TrEMBL
Match: A0A095CAS9 (G2/mitotic-specific cyclin-B3 OS=Schistosoma haematobium OX=6185 GN=MS3_07971 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.864e-10
Identity = 56/181 (30.94%), Postives = 94/181 (51.93%), Query Frame = 1

Query:  997 EELQCNKQFDFMRNSTQ--LTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEG-----LNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSS-FILEMSLHSYTLLLEPESKRAAAAMWLARLI 1515
            E+ Q N   DF+    Q  L   +L    + + E+     L ++ IH A  L+Y +L R   +   +++ +   A+ VA K+E      LN    I D A  K  +  +AE RL+   DF + +P+   F  R+  +  A+S  +  +S F+LE  +HS+T+ L+ ES++AAA +WLAR++
Sbjct:  232 EDFQVN---DFLAAGKQPNLNADMLTTLADWLVEVQENFALNHETIHLAWGLLYAFLDRGQPLARREIQLMACAAIMVACKHEERQMPHLNEFLYITDNAYTKE-EFIEAERRLLIAIDFAVHRPNPYVFLRRYARVLDAHSGPVQFTSRFLLEAGMHSHTISLKRESRKAAAVLWLARVV 408

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BLAST of G2/mitotic-specific cyclin-B3 vs. TrEMBL
Match: A0A183NZS6 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS7612 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.879e-10
Identity = 56/181 (30.94%), Postives = 94/181 (51.93%), Query Frame = 1

Query:  997 EELQCNKQFDFMRNSTQ--LTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEG-----LNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSS-FILEMSLHSYTLLLEPESKRAAAAMWLARLI 1515
            E+ Q N   DF+    Q  L   +L    + + E+     L ++ IH A  L+Y +L R   +   +++ +   A+ VA K+E      LN    I D A  K  +  +AE RL+   DF + +P+   F  R+  +  A+S  +  +S F+LE  +HS+T+ L+ ES++AAA +WLAR++
Sbjct:  237 EDFQVN---DFLAAGKQPNLNADMLTTLADWLVEVQENFALNHETIHLAWGLLYAFLDRGQPLARREIQLMACAAIMVACKHEERQMPHLNEFLYITDNAYTKE-EFIEAERRLLIAIDFAVHRPNPYVFLRRYARVLDAHSGPVQFTSRFLLEAGMHSHTISLKRESRKAAAVLWLARVV 413

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BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Nematostella
Match: EDO39942 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8H6])

HSP 1 Score: 47.3654 bits (111), Expect = 8.753e-6
Identity = 41/154 (26.62%), Postives = 74/154 (48.05%), Query Frame = 1

Query: 1030 MRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEGLNIISNIKDFA-DCKHTDLQQA----ELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPES 1476
            M    +LT S+    ++ + E+     L ++ ++  V ++  YL R + V   +++ V   ++++A K E  +    + DF   C     Q+A    E +++N  +FN+  P    F  RF  +  A+  TL LS FILE +LH Y  ++   S
Sbjct:    1 MEKQKELTISMRAILVDWLVEVQESFELYHETLYLGVRVLDNYLMRSY-VERENLQLVGAVSLYIACKVEERHP-PCLDDFLYICDDAYQQKAFVAMEKKILNSLEFNINMPIPYRFLRRFAKVASADVKTLTLSRFILETTLHHYKFIVHKPS 152

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BLAST of G2/mitotic-specific cyclin-B3 vs. Planmine SMEST
Match: SMESG000001506.1 (SMESG000001506.1)

HSP 1 Score: 1306.2 bits (3379), Expect = 0.000e+0
Identity = 661/664 (99.55%), Postives = 662/664 (99.70%), Query Frame = 1

Query:  112 MNEYNHDIVKYVDENARDEFSAAHFFKNDQGKRNNCISGQALIKKSKNVLADITNNSSTKNRLILRTALTNKMGVLNMKLETSDRTNIENNVINNKPELKRSYXXXXXXXXXXXTVFLPSIRDEINVYPNPIKRCKSFGFANNLEIIEPIFKNFDKESNEMFIGSISHALTTPANGLNKHYKNAITVAVDSPNSTVIRVREGSVAWKVMKETSKIEERVLLTSPRIFCSPREQFVVTKNNQYLSKCLIDSYLTKMNINSSLKSFELFGGSFRKFGHISSLMASNINTSHFHDLHLEELQCNKQFDFMRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEGLNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPESKRAAAAMWLARLIDKFADVRFNEDILCDEELAAQIMNSVWPDYFVQLSGYFHREISNLALKMHVIIIAFISSDEKXXXXXXXXXXXXXVSACHEKYSSLKYGELTTSYEKFTARTVYYASELYCKLNDLQLSTWMDKNEYEILLHAFKENKAKRPFVVSEIPNHKSSNSCPEFIGMTTRYSQMKSAGKPTDKEFKLLTADSGYSTLSND 2103
            MNEYNHDIVKYVDENARDEFSAAHFFKNDQGKRNNCISGQALIKKSKNVLADITNNSSTKNRLILRTALTNKMGVLNMKLETSDRTNIENNVINNKPELKRSYDLSDLNNNDDDTVFLPSIRDEINVYPNPIKRCKSFGFANNLEIIEPIFKNFDKESNEMFIGSISHALTTPANGLNKHYKNAITVAVDSPNSTVIRVREGSVAWKVMKETSKIEERVLLTSPRIFCSPREQFVVTKNNQYLSKCLIDSYLTKMNINSSLKSFELFGGSFRKFGHISSLMASNINTSHFHDLHLEELQCNKQFDFMRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEGLNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPESKRAAAAMWLARLIDKFADVRFNEDILCDEELAAQIMNSVWPDYFVQLSGYFHREISNLALKMHVIIIAFISSDEKHPDISPDPIIHPSVSACHEKYSSLKYGELTTSYEKFTARTVYYASELYC+LNDLQLSTWMDKNEYEILLHAFKENKAKRPFVVSEIPNHKSSNSCPEFIGMTTR  QMKSAGKPTDKEFKLLTADSGYSTLSND
Sbjct:    1 MNEYNHDIVKYVDENARDEFSAAHFFKNDQGKRNNCISGQALIKKSKNVLADITNNSSTKNRLILRTALTNKMGVLNMKLETSDRTNIENNVINNKPELKRSYDLSDLNNNDDDTVFLPSIRDEINVYPNPIKRCKSFGFANNLEIIEPIFKNFDKESNEMFIGSISHALTTPANGLNKHYKNAITVAVDSPNSTVIRVREGSVAWKVMKETSKIEERVLLTSPRIFCSPREQFVVTKNNQYLSKCLIDSYLTKMNINSSLKSFELFGGSFRKFGHISSLMASNINTSHFHDLHLEELQCNKQFDFMRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYLSRVHDVPLVDMRWVTLGAVWVAAKNEGLNIISNIKDFADCKHTDLQQAELRLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLLLEPESKRAAAAMWLARLIDKFADVRFNEDILCDEELAAQIMNSVWPDYFVQLSGYFHREISNLALKMHVIIIAFISSDEKHPDISPDPIIHPSVSACHEKYSSLKYGELTTSYEKFTARTVYYASELYCRLNDLQLSTWMDKNEYEILLHAFKENKAKRPFVVSEIPNHKSSNSCPEFIGMTTRNFQMKSAGKPTDKEFKLLTADSGYSTLSND 664

The following BLAST results are available for this feature:

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Human
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Celegans
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Fly
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Zebrafish
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Xenopus
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Mouse
BLAST of G2/mitotic-specific cyclin-B3 vs. UniProt/SwissProt
BLAST of G2/mitotic-specific cyclin-B3 vs. TrEMBL
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Cavefish
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Sea Lamprey
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Yeast
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Nematostella
BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Medaka
BLAST of G2/mitotic-specific cyclin-B3 vs. Planmine SMEST

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4


Match Name	E-value	Identity	Description
sp\|P36630\|CG22_SCHPO	2.197e-8	29.85	G2/mitotic-specific cyclin cig2 OS=Schizosaccharom...	[more]
sp\|P30284\|CG21_EMENI	6.838e-8	26.42	G2/mitotic-specific cyclin-B OS=Emericella nidulan...	[more]
sp\|P10815\|CG23_SCHPO	4.528e-7	26.94	G2/mitotic-specific cyclin cdc13 OS=Schizosaccharo...	[more]
sp\|Q7XSJ6\|CCB21_ORYSJ	9.054e-6	26.29	Cyclin-B2-1 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of G2/mitotic-specific cyclin-B3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4Z2CRG4	6.785e-11	30.81	G2/mitotic-specific cyclin-B3 OS=Schistosoma japon...	[more]
A0A3P8BQ90	1.433e-10	30.94	Uncharacterized protein OS=Schistosoma margrebowie...	[more]
A0A183MJ56	1.485e-10	30.94	Uncharacterized protein OS=Schistosoma margrebowie...	[more]
A0A095CAS9	2.864e-10	30.94	G2/mitotic-specific cyclin-B3 OS=Schistosoma haema...	[more]
A0A183NZS6	2.879e-10	30.94	Uncharacterized protein OS=Schistosoma mattheei OX...	[more]

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO39942	8.753e-6	26.62	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of G2/mitotic-specific cyclin-B3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G2/mitotic-specific cyclin-B3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000001506.1	0.000e+0	99.55	SMESG000001506.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30021004 ID=SMED30021004|Name=G2/mitotic-specific cyclin-B3|organism=Schmidtea mediterranea sexual|type=transcript|length=2322bp
TTAAATAAACACGCATTTGTAAAATCTAATCTTTGTAGTTTATAACCGAC
TTAATATATTTTAAAAATATATAAAAATATTTCGCTAATTTTAAACAGAT
CAGTGAATAATATGAATGAATATAATCATGATATTGTTAAATATGTTGAC
GAAAATGCTAGGGATGAATTTTCAGCGGCCCATTTTTTTAAAAACGATCA
AGGGAAAAGAAACAATTGTATTTCAGGTCAGGCTTTAATAAAAAAATCAA
AAAATGTATTAGCTGATATCACCAATAATTCCTCCACTAAAAATAGATTA
ATATTGAGGACAGCATTGACAAATAAAATGGGTGTTCTCAACATGAAATT
AGAAACTTCAGATAGAACCAATATTGAAAATAATGTAATAAATAACAAGC
CCGAATTAAAAAGAAGTTACGATCTTTCAGACTTGAATAATAATGATGAC
GATACTGTGTTCTTGCCCAGTATCAGAGATGAAATTAATGTATATCCAAA
TCCAATTAAAAGATGCAAAAGCTTTGGATTTGCTAATAATTTAGAGATAA
TTGAACCCATTTTTAAAAATTTTGATAAAGAATCAAATGAAATGTTTATT
GGTAGTATTTCACATGCTTTGACCACACCGGCTAATGGATTAAATAAACA
TTATAAAAATGCAATTACAGTCGCAGTTGACTCTCCGAATTCTACTGTCA
TAAGAGTCCGTGAAGGCTCCGTGGCTTGGAAAGTAATGAAAGAAACCTCG
AAAATAGAAGAAAGGGTTTTGTTGACATCTCCTAGAATATTTTGTTCACC
TCGAGAACAATTCGTTGTTACAAAAAATAACCAATATCTGTCAAAATGCC
TCATTGATTCATATTTAACAAAAATGAATATTAATTCTTCTTTAAAATCA
TTCGAATTGTTTGGAGGATCTTTTCGAAAATTTGGTCATATTAGTTCTCT
AATGGCTAGCAATATCAATACTTCTCATTTTCATGACTTGCATCTCGAAG
AATTGCAATGCAATAAACAATTTGATTTCATGCGTAACAGTACGCAATTG
ACTGGTAGCATTCTCGGGGGTTTTATCAATGACATATTTGAATTATCAAA
TTGTATTTATCTACCGAATGATGTAATCCATTCTGCGGTTGACTTGATTT
ATGGATATCTCAGTCGAGTTCACGATGTTCCACTAGTCGACATGAGATGG
GTTACACTTGGCGCAGTTTGGGTTGCGGCAAAAAATGAAGGCCTAAATAT
CATATCGAACATCAAAGATTTTGCCGATTGCAAACATACAGACCTGCAAC
AGGCCGAACTGCGGTTGATCAATGTCACTGATTTCAACCTGACAAAGCCG
CATGCTATTAATTTCCGCAATCGATTTCTCCTTATTGAACCAGCAAACTC
GACAACTTTACACCTGTCCAGTTTTATTTTAGAAATGAGCTTGCATAGCT
ATACGCTGCTGTTGGAGCCGGAATCCAAGCGAGCCGCTGCTGCAATGTGG
CTAGCTCGTTTGATAGATAAATTCGCTGACGTCAGATTCAATGAAGATAT
TTTGTGTGACGAAGAATTAGCGGCTCAAATAATGAACTCGGTATGGCCGG
ATTATTTTGTCCAGTTGTCCGGTTATTTCCACCGAGAGATTTCCAATTTG
GCGCTCAAAATGCACGTGATTATCATAGCCTTCATATCGAGCGATGAAAA
GCATCCCGACATCTCGCCAGACCCGATTATCCATCCATCTGTCAGTGCTT
GCCACGAGAAATATTCCAGCCTAAAATACGGCGAATTGACAACATCATAT
GAGAAATTCACCGCTAGAACCGTTTATTATGCCAGTGAACTGTATTGCAA
GCTAAACGATTTACAGCTGTCCACGTGGATGGACAAGAACGAATATGAGA
TTTTATTGCATGCATTTAAGGAAAATAAAGCCAAACGTCCATTTGTCGTG
TCGGAAATTCCCAATCATAAATCCTCGAATTCCTGTCCGGAATTTATTGG
CATGACAACCAGGTATTCCCAAATGAAATCCGCAGGCAAACCCACCGATA
AAGAGTTCAAGTTGCTGACGGCAGATTCTGGCTACAGCACTCTGAGCAAC
GATTAGATATATTTATATATAAATTCATCTGGAATCGATTTTATTAGATT
TTCCACTTTATTATTTTCAACTCAAAATGCTCAAATCGACATGAATGCAT
TTAAAAACAATATTATATCAAAAATTTTATTGTCTTATTGTTTATTTTTT
ATGATAATATTTCACTTCATCAAATTGATTGTTGCATTGTGATTTTGTAG
TACATTTTTTTGCATATATGTT

protein sequence of SMED30021004-orf-1

>SMED30021004-orf-1 ID=SMED30021004-orf-1|Name=SMED30021004-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=665bp
MNEYNHDIVKYVDENARDEFSAAHFFKNDQGKRNNCISGQALIKKSKNVL
ADITNNSSTKNRLILRTALTNKMGVLNMKLETSDRTNIENNVINNKPELK
RSYDLSDLNNNDDDTVFLPSIRDEINVYPNPIKRCKSFGFANNLEIIEPI
FKNFDKESNEMFIGSISHALTTPANGLNKHYKNAITVAVDSPNSTVIRVR
EGSVAWKVMKETSKIEERVLLTSPRIFCSPREQFVVTKNNQYLSKCLIDS
YLTKMNINSSLKSFELFGGSFRKFGHISSLMASNINTSHFHDLHLEELQC
NKQFDFMRNSTQLTGSILGGFINDIFELSNCIYLPNDVIHSAVDLIYGYL
SRVHDVPLVDMRWVTLGAVWVAAKNEGLNIISNIKDFADCKHTDLQQAEL
RLINVTDFNLTKPHAINFRNRFLLIEPANSTTLHLSSFILEMSLHSYTLL
LEPESKRAAAAMWLARLIDKFADVRFNEDILCDEELAAQIMNSVWPDYFV
QLSGYFHREISNLALKMHVIIIAFISSDEKHPDISPDPIIHPSVSACHEK
YSSLKYGELTTSYEKFTARTVYYASELYCKLNDLQLSTWMDKNEYEILLH
AFKENKAKRPFVVSEIPNHKSSNSCPEFIGMTTRYSQMKSAGKPTDKEFK
LLTADSGYSTLSND*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0042025	host cell nucleus
GO:0005634	nucleus

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR039361	Cyclin
IPR036915	Cyclin-like_sf
IPR004367	Cyclin_C-dom
IPR013763	Cyclin-like
IPR006671	Cyclin_N

Vocabulary: **biological process**
Term	Definition
GO:0000079	regulation of cyclin-dependent protein serine/threonine kinase activity
GO:0010389	regulation of G2/M transition of mitotic cell cycle

Vocabulary: **molecular function**
Term	Definition
GO:0019901	protein kinase binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004367	Cyclin, C-terminal domain	SMART	SM01332	Cyclin_C_2	coord: 413..563 e-value: 1.7E-4 score: 31.0
IPR004367	Cyclin, C-terminal domain	PFAM	PF02984	Cyclin_C	coord: 413..558 e-value: 3.7E-7 score: 30.2
None	No IPR available	GENE3D	G3DSA:1.10.472.10		coord: 302..405 e-value: 5.5E-7 score: 31.5
None	No IPR available	GENE3D	G3DSA:1.10.472.10		coord: 406..572 e-value: 8.5E-18 score: 66.7
None	No IPR available	PANTHER	PTHR10177:SF254	CYCLIN-A1	coord: 291..524
IPR006671	Cyclin, N-terminal	PFAM	PF00134	Cyclin_N	coord: 293..410 e-value: 2.1E-6 score: 27.5
IPR039361	Cyclin	PANTHER	PTHR10177	CYCLINE	coord: 291..524
IPR013763	Cyclin-like	CDD	cd00043	CYCLIN	coord: 411..518 e-value: 0.00857476 score: 34.1365
IPR036915	Cyclin-like superfamily	SUPERFAMILY	SSF47954	Cyclin-like	coord: 413..559
IPR036915	Cyclin-like superfamily	SUPERFAMILY	SSF47954	Cyclin-like	coord: 287..410

Main menu

G2/mitotic-specific cyclin-B3

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Owlery

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