Muscarinic acetylcholine receptor

Overview
NameMuscarinic acetylcholine receptor
Smed IDSMED30020987
Length (bp)2566
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Muscarinic acetylcholine receptor (SMED30020987) t-SNE clustered cells

Violin plots show distribution of expression levels for Muscarinic acetylcholine receptor (SMED30020987) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Muscarinic acetylcholine receptor (SMED30020987) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Muscarinic acetylcholine receptor (SMED30020987) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020987

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30020987SMESG000039095.1 SmedASXL_012790SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30020987SMESG000039095.1 dd_Smed_v4_13962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020987SMESG000039095.1 dd_Smed_v4_13962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30020987SMESG000039095.1 dd_Smed_v4_13962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30020987SMESG000039095.1 dd_Smed_v4_13962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30020987SMESG000039095.1 dd_Smed_v6_13962_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Human
Match: CHRM1 (cholinergic receptor muscarinic 1 [Source:HGNC Symbol;Acc:HGNC:1950])

HSP 1 Score: 155.992 bits (393), Expect = 4.023e-40
Identity = 80/199 (40.20%), Postives = 111/199 (55.78%), Query Frame = 2 
Query:  440 AILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDA 1036
            A +G   G++SLATV GNLLV+ SF V   L+   NYF+ SLA  DL+IG FSMN YT YLL+ +W LG + CDLWL+LDY A   S   +  I+ DR+ SV  P  YR  RT  +  +M+   W++   ++   IL W +  G+R     +C+  F +         +  F+L + VMC+LY  IY    N  R+  A
Sbjct:   26 AFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAA 224

HSP 2 Score: 82.4185 bits (202), Expect = 1.073e-15
Identity = 46/108 (42.59%), Postives = 64/108 (59.26%), Query Frame = 2 
Query: 2144 RTRAKPCREP--KEIFERGRTEN--RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAF--LRLLRGDFHR 2449
            R RA   ++P  KE   + +T +  + +KA RT++ IL AF++ WTP++I+VL+  +  D V    + L YWLCY NS INP CYAL N  F+  F  L L R D  R
Sbjct:  334 RDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRR 441
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Human
Match: CHRM1 (cholinergic receptor muscarinic 1 [Source:HGNC Symbol;Acc:HGNC:1950])

HSP 1 Score: 155.992 bits (393), Expect = 4.402e-40
Identity = 80/199 (40.20%), Postives = 111/199 (55.78%), Query Frame = 2 
Query:  440 AILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDA 1036
            A +G   G++SLATV GNLLV+ SF V   L+   NYF+ SLA  DL+IG FSMN YT YLL+ +W LG + CDLWL+LDY A   S   +  I+ DR+ SV  P  YR  RT  +  +M+   W++   ++   IL W +  G+R     +C+  F +         +  F+L + VMC+LY  IY    N  R+  A
Sbjct:   26 AFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAA 224

HSP 2 Score: 82.4185 bits (202), Expect = 1.057e-15
Identity = 46/108 (42.59%), Postives = 64/108 (59.26%), Query Frame = 2 
Query: 2144 RTRAKPCREP--KEIFERGRTEN--RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAF--LRLLRGDFHR 2449
            R RA   ++P  KE   + +T +  + +KA RT++ IL AF++ WTP++I+VL+  +  D V    + L YWLCY NS INP CYAL N  F+  F  L L R D  R
Sbjct:  334 RDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRR 441
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Human
Match: CHRM3 (cholinergic receptor muscarinic 3 [Source:HGNC Symbol;Acc:HGNC:1952])

HSP 1 Score: 156.762 bits (395), Expect = 1.864e-39
Identity = 98/308 (31.82%), Postives = 150/308 (48.70%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDH----RKPTNNMVLDEMTPGEDNPKKSKGFH-WHGNKVDQSGSHQKLKQKKHSEKQD-DRSSSPTFPSDSDIVSIDN 1351
            + FL G+++L T+ GN+LVI SF V + L+   NYF+ SLA  DL+IG+ SMN +T Y+++N W LG + CDLWL++DY A   S   +  I+ DR+ S+  P  YR  RT  +  +M+   W+I   ++   IL W +F GKR  P  EC+  F ++        I  F++ + +M  LY  IY        K   KR K  +            G+  +  E E E F H     +  ++  L + +    N +K    H W   K            K  SE+ D D SSS ++ ++    S++N
Sbjct:   71 IAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIY--------KETEKRTKELA------------GLQASGTEAETENFVHPTGSSRSCSSYELQQQSMKRSNRRKYGRCHFWFTTK----------SWKPSSEQMDQDHSSSDSWNNNDAAASLEN 348

HSP 2 Score: 81.2629 bits (199), Expect = 3.983e-15
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            + +KA +T++ IL AF+I WTP++I+VL+  +    +   F+NL YWLCY NS +NP CYAL N  F+  F  LL
Sbjct:  485 KEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLL 559
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Human
Match: CHRM3 (cholinergic receptor muscarinic 3 [Source:HGNC Symbol;Acc:HGNC:1952])

HSP 1 Score: 156.762 bits (395), Expect = 1.864e-39
Identity = 98/308 (31.82%), Postives = 150/308 (48.70%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDH----RKPTNNMVLDEMTPGEDNPKKSKGFH-WHGNKVDQSGSHQKLKQKKHSEKQD-DRSSSPTFPSDSDIVSIDN 1351
            + FL G+++L T+ GN+LVI SF V + L+   NYF+ SLA  DL+IG+ SMN +T Y+++N W LG + CDLWL++DY A   S   +  I+ DR+ S+  P  YR  RT  +  +M+   W+I   ++   IL W +F GKR  P  EC+  F ++        I  F++ + +M  LY  IY        K   KR K  +            G+  +  E E E F H     +  ++  L + +    N +K    H W   K            K  SE+ D D SSS ++ ++    S++N
Sbjct:   71 IAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIY--------KETEKRTKELA------------GLQASGTEAETENFVHPTGSSRSCSSYELQQQSMKRSNRRKYGRCHFWFTTK----------SWKPSSEQMDQDHSSSDSWNNNDAAASLEN 348

HSP 2 Score: 81.2629 bits (199), Expect = 3.983e-15
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            + +KA +T++ IL AF+I WTP++I+VL+  +    +   F+NL YWLCY NS +NP CYAL N  F+  F  LL
Sbjct:  485 KEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLL 559
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Human
Match: CHRM4 (cholinergic receptor muscarinic 4 [Source:HGNC Symbol;Acc:HGNC:1953])

HSP 1 Score: 149.828 bits (377), Expect = 8.769e-38
Identity = 92/299 (30.77%), Postives = 139/299 (46.49%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVAL------NLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPS 1324
            +  + G +SL TV GN+LV+ S  V R L+   NYF+ SLA  DL+IG FSMN YT+Y++  YWPLG + CDLWL+LDY     S   +  I+ DR+  V  P  Y   RT     +M+AA W++   ++   IL W F  GKR  P N+C+  F ++        I  F+L +++M  LY    H++L      +  R    K +K  +L  +    M +        E   E  + R            P    P   K      N+     + Q  K++  +E     +++P  P+
Sbjct:   35 IATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLY---IHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAARE--ELRNGKLEEAPPPALPPPPRPVADKD---TSNESSSGSATQNTKERPATELSTTEATTPAMPA 325

HSP 2 Score: 82.0333 bits (201), Expect = 1.523e-15
Identity = 37/90 (41.11%), Postives = 55/90 (61.11%), Query Frame = 2 
Query: 2177 EIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            ++ ++ +   R RK  RTI  IL AF++ WTP++++VL+  +    +    +++ YWLCY NS INP CYAL NA FKK F  LL   + 
Sbjct:  384 QVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYR 473
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Celegans
Match: gar-2 (Muscarinic acetylcholine receptor gar-2 [Source:UniProtKB/Swiss-Prot;Acc:Q09388])

HSP 1 Score: 187.193 bits (474), Expect = 1.121e-53
Identity = 96/213 (45.07%), Postives = 133/213 (62.44%), Query Frame = 2 
Query:  437 TAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYT-IYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGK-RGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSM 1069
            + +L      +S+ TV GNL V+ S+++++ +R PTNYFI SLA++DLLIG+  +  YT  YL  N W  G++ CDLWLS+DY  CL S YTV  ITVDR+ SV  PA YRNWRT  +V++++   W++PS +F   I G+  FTG  R   + EC+  F T+ + N  + I Y+W TL VM  LY GIY  A  L  KSD K +++  L  M
Sbjct:    5 SVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIPVYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYWTTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEM 217
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Celegans
Match: gar-2 (Muscarinic acetylcholine receptor gar-2 [Source:UniProtKB/Swiss-Prot;Acc:Q09388])

HSP 1 Score: 194.512 bits (493), Expect = 1.446e-52
Identity = 96/214 (44.86%), Postives = 133/214 (62.15%), Query Frame = 2 
Query:  434 VTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYT-IYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGK-RGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSM 1069
             + +L      +S+ TV GNL V+ S+++++ +R PTNYFI SLA++DLLIG+  +  YT  YL  N W  G++ CDLWLS+DY  CL S YTV  ITVDR+ SV  PA YRNWRT  +V++++   W++PS +F   I G+  FTG  R   + EC+  F T+ + N  + I Y+W TL VM  LY GIY  A  L  KSD K +++  L  M
Sbjct:    4 ASVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIPVYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYWTTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEM 217

HSP 2 Score: 115.931 bits (289), Expect = 1.750e-26
Identity = 59/121 (48.76%), Postives = 79/121 (65.29%), Query Frame = 2 
Query: 2099 RVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFN----LSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            +VR +I Q+    +H  R+K  +  K    + R+ENRARKALRTIT ILG+F+I WTPF++L  + G+         FN    +SY+LCY NSP+NPFCYA+AN QFKK   R+ +GDF R
Sbjct:  497 KVRRLITQMR---SHSIRSKR-KANKNKEYKSRSENRARKALRTITFILGSFIILWTPFYVLATIYGFCETCKASPSFNTLYTISYYLCYMNSPLNPFCYAMANQQFKKTLTRIFKGDFRR 613
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Celegans
Match: gar-2 (Muscarinic acetylcholine receptor gar-2 [Source:UniProtKB/Swiss-Prot;Acc:Q09388])

HSP 1 Score: 194.512 bits (493), Expect = 1.811e-52
Identity = 96/214 (44.86%), Postives = 133/214 (62.15%), Query Frame = 2 
Query:  434 VTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYT-IYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGK-RGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSM 1069
             + +L      +S+ TV GNL V+ S+++++ +R PTNYFI SLA++DLLIG+  +  YT  YL  N W  G++ CDLWLS+DY  CL S YTV  ITVDR+ SV  PA YRNWRT  +V++++   W++PS +F   I G+  FTG  R   + EC+  F T+ + N  + I Y+W TL VM  LY GIY  A  L  KSD K +++  L  M
Sbjct:    4 ASVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIPVYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYWTTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEM 217

HSP 2 Score: 113.62 bits (283), Expect = 8.227e-26
Identity = 51/91 (56.04%), Postives = 65/91 (71.43%), Query Frame = 2 
Query: 2189 RGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFN----LSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            + R+ENRARKALRTIT ILG+F+I WTPF++L  + G+         FN    +SY+LCY NSP+NPFCYA+AN QFKK   R+ +GDF R
Sbjct:  536 KSRSENRARKALRTITFILGSFIILWTPFYVLATIYGFCETCKASPSFNTLYTISYYLCYMNSPLNPFCYAMANQQFKKTLTRIFKGDFRR 626
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Celegans
Match: gar-2 (Muscarinic acetylcholine receptor gar-2 [Source:UniProtKB/Swiss-Prot;Acc:Q09388])

HSP 1 Score: 194.897 bits (494), Expect = 2.360e-52
Identity = 96/214 (44.86%), Postives = 133/214 (62.15%), Query Frame = 2 
Query:  434 VTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYT-IYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGK-RGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSM 1069
             + +L      +S+ TV GNL V+ S+++++ +R PTNYFI SLA++DLLIG+  +  YT  YL  N W  G++ CDLWLS+DY  CL S YTV  ITVDR+ SV  PA YRNWRT  +V++++   W++PS +F   I G+  FTG  R   + EC+  F T+ + N  + I Y+W TL VM  LY GIY  A  L  KSD K +++  L  M
Sbjct:    4 ASVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIPVYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYWTTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEM 217

HSP 2 Score: 115.161 bits (287), Expect = 3.122e-26
Identity = 57/115 (49.57%), Postives = 73/115 (63.48%), Query Frame = 2 
Query: 2117 QQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFN----LSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            Q ++  +    R K  RE K      R+ENRARKALRTIT ILG+F+I WTPF++L  + G+         FN    +SY+LCY NSP+NPFCYA+AN QFKK   R+ +GDF R
Sbjct:  557 QSVLSALNFFQRKKVTREYK-----SRSENRARKALRTITFILGSFIILWTPFYVLATIYGFCETCKASPSFNTLYTISYYLCYMNSPLNPFCYAMANQQFKKTLTRIFKGDFRR 666
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Celegans
Match: gar-1 (Probable muscarinic acetylcholine receptor gar-1 [Source:UniProtKB/Swiss-Prot;Acc:Q18007])

HSP 1 Score: 166.777 bits (421), Expect = 4.926e-43
Identity = 82/216 (37.96%), Postives = 133/216 (61.57%), Query Frame = 2 
Query:  467 VSLATVAGNLLVITSFFVERAL-RNPTNYFIASLAVTDLLIGIFSMNFYTIYLL-LNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKR-GCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGM 1105
            +SL T+ GN +V+ ++ +ER + +  +N +I SLA++DL+IGI    F+T+Y+L  + WPLG + C  WL LDYT CL S  TV  IT DR+ SV   AKY  W++  K  +++  +W++P+ IF   I GW   TG+       EC A F ++ + N  + + Y+W TL+ M  LY GI+  A NL++K+ AK R+  +L+ ++ +  +++GV +
Sbjct:   34 LSLETIIGNAMVVMAYRIERNISKQVSNRYIVSLAISDLIIGIEGFPFFTVYVLNGDRWPLGWVACQTWLFLDYTLCLVSILTVLLITADRYLSVCHTAKYLKWQSPTKTQLLIVMSWLLPAIIFGIMIYGWQAMTGQSTSMSGAECSAPFLSNPYVNMGMYVAYYWTTLVAMLILYKGIHQAAKNLEKKAKAKERRHIALI-LSQRLGTQVGVSL 248

HSP 2 Score: 99.7525 bits (247), Expect = 2.460e-21
Identity = 45/98 (45.92%), Postives = 64/98 (65.31%), Query Frame = 2 
Query: 2171 PKEIFERGR----TENRARKALRTITLILGAFVICWTPFHILVLLKGYYP-DLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            P  +F RGR     E RA KA RTIT I+G F I W+P++I+  + G+   + +    + LSY++CY NS  NPF YALAN QF+ AF+R+ RG+F++
Sbjct:  523 PIAVFNRGRKQTKAEKRAHKAFRTITFIVGFFAILWSPYYIMATVYGFCKGECIPSFLYTLSYYMCYLNSSGNPFAYALANRQFRSAFMRMFRGNFNK 620
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Fly
Match: mAChR-B (gene:FBgn0037546 transcript:FBtr0344726)

HSP 1 Score: 268.855 bits (686), Expect = 7.185e-78
Identity = 138/313 (44.09%), Postives = 191/313 (61.02%), Query Frame = 2 
Query:  479 TVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRI-----GVGM-------TNDENEDEIFDHRKPTNNMVLDEMTPGEDNP-----KKSKGFHWHGNKVDQSGSHQKLKQKKHS--EKQDDRSSSPTFPSDSDIVSIDNLQQ 1360
            TV GN+LV+ +F V+R +R P+NYFIASLA TD+LIG  SM FYTIY+L  YW LG M CDLWLS+DYT CL SQYTV  IT+DR+CSV I AKYR+WRT  +V+ MV  TWIIP+ +FF  I GW  FTGKR     +C   F  D  FNT L IGY+W TLIV+  LY GIY  A ++Q++S+AK+RKM S+V+++   MS +     G+G+       T  E   +  D       ++      G+ NP     ++ +       +   +   ++ K+++ +   ++ +RSSSP F SD +  S++  QQ
Sbjct:  147 TVGGNILVLLAFIVDRNIRQPSNYFIASLAATDMLIGTVSMPFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRTRTRVIYMVTITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTLIVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAGDQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREAAVDAEKSERSSSPAFDSDEE-SSVNQAQQ 458

HSP 2 Score: 133.65 bits (335), Expect = 9.006e-32
Identity = 55/91 (60.44%), Postives = 72/91 (79.12%), Query Frame = 2 
Query: 2186 ERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGY--YPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            ++ ++ENRARKA RTI+ ILG FV CWTP+H+L L++G+  +P  +N H +  SY+LCYANSP+NPFCYALAN QFKK F R+L+GD H T
Sbjct:  665 QKSKSENRARKAFRTISFILGCFVACWTPYHVLALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRILKGDLHMT 755
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Fly
Match: mAChR-B (gene:FBgn0037546 transcript:FBtr0081789)

HSP 1 Score: 270.011 bits (689), Expect = 1.370e-76
Identity = 138/313 (44.09%), Postives = 191/313 (61.02%), Query Frame = 2 
Query:  479 TVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRI-----GVGM-------TNDENEDEIFDHRKPTNNMVLDEMTPGEDNP-----KKSKGFHWHGNKVDQSGSHQKLKQKKHS--EKQDDRSSSPTFPSDSDIVSIDNLQQ 1360
            TV GN+LV+ +F V+R +R P+NYFIASLA TD+LIG  SM FYTIY+L  YW LG M CDLWLS+DYT CL SQYTV  IT+DR+CSV I AKYR+WRT  +V+ MV  TWIIP+ +FF  I GW  FTGKR     +C   F  D  FNT L IGY+W TLIV+  LY GIY  A ++Q++S+AK+RKM S+V+++   MS +     G+G+       T  E   +  D       ++      G+ NP     ++ +       +   +   ++ K+++ +   ++ +RSSSP F SD +  S++  QQ
Sbjct:  147 TVGGNILVLLAFIVDRNIRQPSNYFIASLAATDMLIGTVSMPFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRTRTRVIYMVTITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTLIVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAGDQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREAAVDAEKSERSSSPAFDSDEE-SSVNQAQQ 458

HSP 2 Score: 135.191 bits (339), Expect = 4.797e-32
Identity = 55/91 (60.44%), Postives = 72/91 (79.12%), Query Frame = 2 
Query: 2186 ERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGY--YPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            ++ ++ENRARKA RTI+ ILG FV CWTP+H+L L++G+  +P  +N H +  SY+LCYANSP+NPFCYALAN QFKK F R+L+GD H T
Sbjct:  929 QKSKSENRARKAFRTISFILGCFVACWTPYHVLALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRILKGDLHMT 1019
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Fly
Match: mAChR-A (gene:FBgn0000037 transcript:FBtr0072367)

HSP 1 Score: 155.606 bits (392), Expect = 9.489e-39
Identity = 109/408 (26.72%), Postives = 186/408 (45.59%), Query Frame = 2 
Query:  422 FPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGF-QTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDH-------------------RKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKK--------------HS--EKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVIL--DDSPSPSETSPF-NFFQYGNMSTVTTTSSS 1528
            + L    ++GF+A ++S  TVAGN++V+ SF +++ L+  +NYF+ SLA+ D  IG  SM  + +  +L YWPLG + CD WL+LDY A   S   +  I+ DR+ SV  P  YR  RT N+  +M+ A W I   ++   I  WP+  GKR  P++EC+  F +T+ +      +  F+  + +MC LY  I+       R++  +++ + +L +    +  R     +N  +E+ + +H                   R+P +    D  +    N     G+H   ++     S   +K+                HS  E  DD +     PSD    S++ ++       +V            ++ P IL  D SP+P    P  +  Q   MS VT ++++
Sbjct:   99 YSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIW-------RETKKRQKDLPNLQAGKKDSSKR-----SNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGCTSMNVMRDNYSMGGSV----------SGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTA 484

HSP 2 Score: 75.8702 bits (185), Expect = 1.041e-13
Identity = 35/79 (44.30%), Postives = 52/79 (65.82%), Query Frame = 2 
Query: 2216 KALRTITLILGAFVICWTPFHILVLLKGYY--PDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            KA +T++ IL +F+I WTP++ILVL+K      D +    ++  Y LCY NS INP CYAL NA F++ ++R+L   +H
Sbjct:  697 KAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRTYVRILTCKWH 775
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Fly
Match: mAChR-A (gene:FBgn0000037 transcript:FBtr0072368)

HSP 1 Score: 155.221 bits (391), Expect = 1.204e-38
Identity = 78/241 (32.37%), Postives = 133/241 (55.19%), Query Frame = 2 
Query:  422 FPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGF-QTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDH 1141
            + L    ++GF+A ++S  TVAGN++V+ SF +++ L+  +NYF+ SLA+ D  IG  SM  + +  +L YWPLG + CD WL+LDY A   S   +  I+ DR+ SV  P  YR  RT N+  +M+ A W I   ++   I  WP+  GKR  P++EC+  F +T+ +      +  F+  + +MC LY  I+       R++  +++ + +L +    +  R     +N  +E+ + +H
Sbjct:   99 YSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIW-------RETKKRQKDLPNLQAGKKDSSKR-----SNSSDENTVVNH 327

HSP 2 Score: 76.2554 bits (186), Expect = 8.522e-14
Identity = 35/79 (44.30%), Postives = 52/79 (65.82%), Query Frame = 2 
Query: 2216 KALRTITLILGAFVICWTPFHILVLLKGYY--PDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            KA +T++ IL +F+I WTP++ILVL+K      D +    ++  Y LCY NS INP CYAL NA F++ ++R+L   +H
Sbjct:  714 KAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRTYVRILTCKWH 792
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Fly
Match: 5-HT1A (gene:FBgn0004168 transcript:FBtr0474126)

HSP 1 Score: 108.997 bits (271), Expect = 6.393e-24
Identity = 69/219 (31.51%), Postives = 106/219 (48.40%), Query Frame = 2 
Query:  353 SGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVA 1009
            +G LN TT+     +++ + T L  + VT++L    G++ L T+ GN+ VI +  +ER L+N  NY +ASLAV DL +    M    +Y +   W LG   CD+W S D   C  S   +  I VDR+ +V     Y + RT N+V +M+   W     +      GW      +   Q +C      DV +    T   F++ L+V+ +LY  IY  A
Sbjct:  205 TGGLNVTTSK----VAEDDFTQLLRMAVTSVL---LGLMILVTIIGNVFVIAAIILERNLQNVANYLVASLAVADLFVACLVMPLGAVYEISQGWILGPELCDIWTSCDVLCCTASILHLVAIAVDRYWAV-TNIDYIHSRTSNRVFMMIFCVWTAAVIVSLAPQFGWKDPDYLQRIEQQKCM--VSQDVSYQVFATCCTFYVPLLVILALYWKIYQTA 413

HSP 2 Score: 64.3142 bits (155), Expect = 3.957e-10
Identity = 35/93 (37.63%), Postives = 51/93 (54.84%), Query Frame = 2 
Query: 2174 KEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDL-VNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            KE  E      R RKA +T+ +I GAFV+CW PF ++ L         ++    +L  WL Y NS +NP  Y + + +F++AF R+L G  HR
Sbjct:  738 KETLE----AKRERKAAKTLAIITGAFVVCWLPFFVMALTMPLCAACQISDSVASLFLWLGYFNSTLNPVIYTIFSPEFRQAFKRILFGG-HR 825
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Zebrafish
Match: chrm1a (cholinergic receptor, muscarinic 1a [Source:ZFIN;Acc:ZDB-GENE-090410-9])

HSP 1 Score: 152.91 bits (385), Expect = 3.278e-39
Identity = 80/213 (37.56%), Postives = 123/213 (57.75%), Query Frame = 2 
Query:  437 TAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAG 1075
            TA++ F+   +SL T+ GN+LV+ SF V   LR  +NYF+ SLAV D+++G  SMN YT Y+LLN W LG + CD+WL++DY A   S   +  I++DR+ SV  P  YR  RT  + +IM++  W I   I+   IL W +  G+R   +NEC   F ++        I  F+L + VM +LY  +Y       R+++ + +K+  L++  G
Sbjct:   22 TAMITFITVPLSLITITGNVLVMISFRVNPLLRTVSNYFLLSLAVADVILGSISMNLYTTYILLNGWTLGNLACDVWLAVDYVASNASVMNLLAISIDRYLSVMRPLTYRAKRTPKRAMIMISLAWTISFVIWAPAILFWQYIVGERTVQENECSIQFLSEPVITFGTAIAAFYLPVSVMVALYWRVY-------RETEKRSQKLAGLMASQG 227

HSP 2 Score: 80.4925 bits (197), Expect = 2.910e-15
Identity = 36/84 (42.86%), Postives = 53/84 (63.10%), Query Frame = 2 
Query: 2186 ERGRTEN--RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
             + RT +  + +KA RT++ IL AF++ WTP++I+VL+  +  + V    + L YWLCY NS +NP CYAL N  F+  F  LL
Sbjct:  363 RKARTSSLIKEKKAARTLSAILLAFIVTWTPYNIMVLVSTFCDNCVPERLWKLGYWLCYVNSTVNPLCYALCNKHFRVTFRALL 446
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Zebrafish
Match: chrm1a (cholinergic receptor, muscarinic 1a [Source:ZFIN;Acc:ZDB-GENE-090410-9])

HSP 1 Score: 151.754 bits (382), Expect = 3.794e-39
Identity = 85/225 (37.78%), Postives = 129/225 (57.33%), Query Frame = 2 
Query:  437 TAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLV-SMAGQTMSRIGVGMT 1108
            TA++ F+   +SL T+ GN+LV+ SF V   LR  +NYF+ SLAV D+++G  SMN YT Y+LLN W LG + CD+WL++DY A   S   +  I++DR+ SV  P  YR  RT  + +IM++  W I   I+   IL W +  G+R   +NEC   F ++        I  F+L + VM +LY  +Y       R+++ + +K+  L+ S  G+T +   VG T
Sbjct:   22 TAMITFITVPLSLITITGNVLVMISFRVNPLLRTVSNYFLLSLAVADVILGSISMNLYTTYILLNGWTLGNLACDVWLAVDYVASNASVMNLLAISIDRYLSVMRPLTYRAKRTPKRAMIMISLAWTISFVIWAPAILFWQYIVGERTVQENECSIQFLSEPVITFGTAIAAFYLPVSVMVALYWRVY-------RETEKRSQKLAGLMASQGGRTGNASQVGFT 239

HSP 2 Score: 79.7221 bits (195), Expect = 3.271e-15
Identity = 36/84 (42.86%), Postives = 53/84 (63.10%), Query Frame = 2 
Query: 2186 ERGRTEN--RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
             + RT +  + +KA RT++ IL AF++ WTP++I+VL+  +  + V    + L YWLCY NS +NP CYAL N  F+  F  LL
Sbjct:  312 RKARTSSLIKEKKAARTLSAILLAFIVTWTPYNIMVLVSTFCDNCVPERLWKLGYWLCYVNSTVNPLCYALCNKHFRVTFRALL 395
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Zebrafish
Match: CR812847.1 (pep chromosome:GRCz11:CHR_ALT_CTG14_1_19:24256795:24262094:1 gene:ENSDARG00000112321.1 transcript:ENSDART00000181335.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR812847.1)

HSP 1 Score: 150.984 bits (380), Expect = 4.504e-39
Identity = 85/225 (37.78%), Postives = 129/225 (57.33%), Query Frame = 2 
Query:  437 TAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLV-SMAGQTMSRIGVGMT 1108
            TA++ F+   +SL T+ GN+LV+ SF V   LR  +NYF+ SLAV D+++G  SMN YT Y+LLN W LG + CD+WL++DY A   S   +  I++DR+ SV  P  YR  RT  + +IM++  W I   I+   IL W +  G+R   +NEC   F ++        I  F+L + VM +LY  +Y       R+++ + +K+  L+ S  G+T +   VG T
Sbjct:   19 TAMITFITVPLSLITITGNVLVMISFRVNPLLRTVSNYFLLSLAVADVILGSISMNLYTTYILLNGWTLGNLACDVWLAVDYVASNASVMNLLAISIDRYLSVMRPLTYRAKRTPKRAMIMISLAWTISFVIWAPAILFWQYIVGERTVQENECSIQFLSEPVITFGTAIAAFYLPVSVMVALYWRVY-------RETEKRSQKLAGLMASQGGRTGNASQVGFT 236

HSP 2 Score: 79.7221 bits (195), Expect = 3.366e-15
Identity = 36/84 (42.86%), Postives = 53/84 (63.10%), Query Frame = 2 
Query: 2186 ERGRTEN--RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
             + RT +  + +KA RT++ IL AF++ WTP++I+VL+  +  + V    + L YWLCY NS +NP CYAL N  F+  F  LL
Sbjct:  302 RKARTSSLIKEKKAARTLSAILLAFIVTWTPYNIMVLVSTFCDNCVPERLWKLGYWLCYVNSTVNPLCYALCNKHFRVTFRALL 385
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Zebrafish
Match: chrm3a (cholinergic receptor, muscarinic 3a [Source:ZFIN;Acc:ZDB-GENE-090410-4])

HSP 1 Score: 151.754 bits (382), Expect = 6.104e-38
Identity = 103/376 (27.39%), Postives = 167/376 (44.41%), Query Frame = 2 
Query:  461 GVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKK------SKGFH---WHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDL-----IITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYRQM 1546
            G++SL T+ GN+LV+ SF V R L+   NYF+ SLAV DL+IG+ SMN YT Y+++  W +G   CDLWL++DY A   S   +  I+ DR+ S+  P  YR  RT  +  +M+   W +   ++   IL W +F G+R  PQ++C+  F ++        +  F+L + +M  LY  IY       ++++ + R++  L    G    R G              H    ++        G+   KK      S  FH   W     D+SG +++  Q       ++ +      S S     D  +  P TT  +      L      +  ++ S   LD    P  ++     + G++S   T  + RF   M
Sbjct:   76 GLLSLITIIGNILVMVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGQWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWFVSLILWAPAILFWQYFVGQRTVPQDKCYIQFLSEPIITFCTAMAAFYLPVTIMSVLYWRIY-------KETENRSRELAGLQGSGG----RFG-----GVERPRFHLHATRGSSRSCSSFELGQPGHKKASVHSLSGRFHCWGWKSGGSDKSGPNREADQSSSDSWNNNDAGLSADHSGSS----DEDESAPSTTRAIFSIVLSLPGVRAAVNSQVTSCEELDTEEDPLRSAEEKDSRDGSISRSVTNGNKRFVGGM 431

HSP 2 Score: 77.0258 bits (188), Expect = 5.133e-14
Identity = 36/91 (39.56%), Postives = 55/91 (60.44%), Query Frame = 2 
Query: 2162 CREPKEIFERGRTE-NRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
             R   +I +R R    + +KA +T++ IL AF+I WTP++I+VL+  +    +  + + L YWLCY NS +NP CYAL N  F+  F  +L
Sbjct:  475 ARARTQITKRKRMSLIKEKKAAQTLSAILFAFIITWTPYNIMVLVNTFCNGCIPENLWALGYWLCYVNSTVNPMCYALCNKTFRSTFKMIL 565
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Zebrafish
Match: chrm2a (cholinergic receptor, muscarinic 2a [Source:ZFIN;Acc:ZDB-GENE-030314-1])

HSP 1 Score: 149.828 bits (377), Expect = 6.413e-38
Identity = 85/243 (34.98%), Postives = 126/243 (51.85%), Query Frame = 2 
Query:  452 FLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMT 1180
             +AG +SL TV GN+LV+ S  V R+L+   NYF+ SLA  DL+IG+ SMN YT+Y+++ YWPLG + CDLWL+LDY     S   +  I+ DR+  V  P  Y   RT     +M+AA W++   ++   IL W F  G R  P+ EC+  F ++        I  F+L +I+M  LY  +   + +  +K + K             +   + V  +N   E+      KPTNN +  E T
Sbjct:   36 LVAGSLSLVTVIGNILVMLSIKVNRSLQTVNNYFLFSLACADLIIGLCSMNLYTVYIVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLSYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVPEKECYIQFFSNAAVTFGTAIAAFYLPVIIMMVLYWQVSRASKSRVKKDNRK------------PSGGNLDVASSNQIREN---SANKPTNNNLTAEET 263

HSP 2 Score: 79.7221 bits (195), Expect = 5.663e-15
Identity = 36/76 (47.37%), Postives = 48/76 (63.16%), Query Frame = 2 
Query: 2204 NRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            +R +K  RTI  IL AFV  WTP++++VL+  +    +    + + YWLCY NS INP CYAL N  FKK F +LL
Sbjct:  409 SREKKVTRTIMAILVAFVATWTPYNVMVLINTFCSSCIPNTVWTIGYWLCYINSTINPACYALCNITFKKTFKQLL 484
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Xenopus
Match: ENSXETT00000048394.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:5:59937956:59939943:1 gene:ENSXETG00000020181.1 transcript:ENSXETT00000048394.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 152.525 bits (384), Expect = 3.020e-38
Identity = 74/212 (34.91%), Postives = 121/212 (57.08%), Query Frame = 2 
Query:  443 ILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQ 1078
            ++   +G+++L TV GN+LVI +F V + L+   NYF+ SLA  DL+IG+ SMN +T Y+++++W +G + CDLWL +DY A   S   +  I+ DR+ S+  P  YR  RT  +  IM+   WII   ++   IL W +F GKR  P +EC+  F T+        I  F+L + +M  LY  IY       ++++ + +++  L +  G+
Sbjct:   51 LIASFSGIIALVTVIGNILVILAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMDHWIMGSLACDLWLMIDYVASNASVMNLLVISFDRYFSITRPLTYRTKRTTKRAGIMIGLAWIISFILWAPAILFWQYFVGKRTVPSDECFIQFFTEPIITFGTAIAAFYLPVSIMTILYWRIY-------KETEKRTKELAGLQASGGK 255

HSP 2 Score: 77.411 bits (189), Expect = 5.015e-14
Identity = 34/75 (45.33%), Postives = 49/75 (65.33%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            + +KA +T++ IL AF+I W P++I+VL+  +    +   ++NL YWLCY NS INP CYAL N  F+  F  LL
Sbjct:  456 KEKKAAQTLSAILLAFIITWAPYNIMVLVNTFCESCIPRIYWNLGYWLCYINSTINPICYALCNKTFRTTFKILL 530
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Xenopus
Match: CHRM2 (cholinergic receptor, muscarinic 2 [Source:NCBI gene;Acc:100485603])

HSP 1 Score: 149.058 bits (375), Expect = 1.008e-37
Identity = 94/308 (30.52%), Postives = 146/308 (47.40%), Query Frame = 2 
Query:  452 FLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFP--SDSDIVSIDNLQQGPK 1369
             +AG +SL T+ GN+LV+ S  V R L+   NYF+ SLA  DL+IG+FSMN YT+Y ++ YWP+G + CDLWL+LDY     S   +  I+ DR+  V  P  Y   RT     +M+AA W++   ++   IL W F  G R  P+ EC+  F ++        I  F+L +I+M  LY  I   + +  +K   +       +S +      +     N    ++  DH K  N  V      G +N  +++G     +    S S    K +  ++     S S   P   +S +  I  + + PK
Sbjct:   31 IVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPIGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVRRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVPEGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWQISRASKSRIKKGKKEAAPNQDPISPSNVQGKIVKPNNNNVSANEDGLDHGKIQNGKVTSN--SGIENCVQAEGEEKEISNDSTSVSVVNTKDEGAAKDVPQASGSQANPKTENSKLTCIRIVTKSPK 336

HSP 2 Score: 80.1073 bits (196), Expect = 4.437e-15
Identity = 37/81 (45.68%), Postives = 49/81 (60.49%), Query Frame = 2 
Query: 2204 NRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            +R +K  RTI  IL AF+I WTP++++VL+  +    V    + + YWLCY NS INP CYAL N  FKK F  LL   + 
Sbjct:  384 SREKKVTRTILAILLAFIITWTPYNVMVLINTFCAVCVPNTVWTIGYWLCYINSTINPACYALCNTTFKKTFKHLLMCQYK 464
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Xenopus
Match: slitrk3 (SLIT and NTRK like family member 3 [Source:Xenbase;Acc:XB-GENE-964617])

HSP 1 Score: 144.436 bits (363), Expect = 7.876e-36
Identity = 74/185 (40.00%), Postives = 103/185 (55.68%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIY 1000
            +  ++ +VSL T+ GN+LV+ SF V   L+   NY++ SLA  DL+IGIFSMN YT Y+L+ +W LG + CDLWL+LDY A   S   +  I+ DR+ S+  P  YR  RT  +  +M+   W I   ++   IL W +F G R  P  EC   F  +        I  F+L + VM  LY  IY
Sbjct:   28 IATVSAIVSLITIVGNILVMVSFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTSYILIGHWSLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGLMIGLAWFISFILWAPAILCWQYFVGGRTVPPEECQIQFLYEPIITFGTAIAAFYLPVSVMTILYCRIY 212

HSP 2 Score: 84.7297 bits (208), Expect = 2.113e-16
Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            + RKA +T++ IL AF+I WTP++I+VL+  +  D +    ++L YWLCY NS +NP CYAL N  F+K F  LL
Sbjct:  424 KERKAAQTLSAILLAFIITWTPYNIMVLVSTFCSDCIPSSLWHLGYWLCYVNSTVNPICYALCNKTFRKTFKMLL 498
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Xenopus
Match: CHRM1 (cholinergic receptor, muscarinic 1 [Source:NCBI gene;Acc:100490434])

HSP 1 Score: 132.88 bits (333), Expect = 5.433e-32
Identity = 68/200 (34.00%), Postives = 107/200 (53.50%), Query Frame = 2 
Query:  461 GVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSL 1060
            G++SL TV GN+LV+ +F V   L+   NYF+ SLA  D++IG  SMN YT Y+++  W LG + CDLWL+LDY A   S   +  I+ DR+ S+  P  YR  RT  +  IM+   W I   ++   I+ W +  G+R     +C+  F +         I  F+L + +M  LY  +Y       R+++ + +++  L
Sbjct:   55 GILSLVTVIGNVLVLLAFKVNSDLKTVNNYFLLSLACADIIIGALSMNLYTTYIVMGRWALGPVSCDLWLALDYVASNASVMNLLIISFDRYFSITRPLTYRAKRTPRRAAIMIGMAWFISFVLWAPAIIFWQYIVGERTVEATDCYIQFFSQPIITFGTAIAAFYLPVTIMIILYWRVY-------RETENRSKELAGL 247

HSP 2 Score: 84.3445 bits (207), Expect = 2.906e-16
Identity = 35/75 (46.67%), Postives = 51/75 (68.00%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            + +KA +T++ IL AF++ WTP++I+VL+  + PD V    + L YWLCY NS +NP CYAL N  F++ F  LL
Sbjct:  423 KEKKAAKTLSAILLAFILTWTPYNIMVLVSTFCPDCVPKKLWELGYWLCYVNSTVNPMCYALCNRSFRRTFKMLL 497
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Xenopus
Match: cdkn2aip (CDKN2A interacting protein [Source:Xenbase;Acc:XB-GENE-984876])

HSP 1 Score: 121.324 bits (303), Expect = 2.117e-29
Identity = 79/229 (34.50%), Postives = 121/229 (52.84%), Query Frame = 2 
Query:  479 TVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTD-NKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNM 1162
            TV GN LVI +FFVE+ LRN +N+F+ +L++ D ++G F++  Y  YLL   W LG+  C LWL +DYT C  S + V  I+ DRF SV     YR+ +    + +I +AA WI+   ++   I+ W F       P+N C AGF    ++    +   F L LI +      IY    N++++S   RRKM + +S+  Q  SR    + N      ++  R  +NN+
Sbjct:   44 TVGGNTLVILAFFVEKRLRNQSNFFLLNLSIADFILGTFAIPLYVPYLLTGKWLLGKFLCKLWLIVDYTMCTASAFNVALISWDRFLSVTQAVLYRSQQNRPCRTVIKMAAVWILSFLLYGPAIIFWDFVPSTEEIPENICVAGFYYTWYYLLTASAFDFVLPLISISFFNLSIY---CNIKKRS---RRKMQNSISLPPQK-SRKEAKLYN-----RLYQKRLCSNNL 260
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Mouse
Match: Chrm1 (cholinergic receptor, muscarinic 1, CNS [Source:MGI Symbol;Acc:MGI:88396])

HSP 1 Score: 156.377 bits (394), Expect = 2.466e-40
Identity = 80/199 (40.20%), Postives = 111/199 (55.78%), Query Frame = 2 
Query:  440 AILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDA 1036
            A +G   G++SLATV GNLLV+ SF V   L+   NYF+ SLA  DL+IG FSMN YT YLL+ +W LG + CDLWL+LDY A   S   +  I+ DR+ SV  P  YR  RT  +  +M+   W++   ++   IL W +  G+R     +C+  F +         +  F+L + VMC+LY  IY    N  R+  A
Sbjct:   26 AFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAA 224

HSP 2 Score: 80.8777 bits (198), Expect = 2.228e-15
Identity = 38/83 (45.78%), Postives = 52/83 (62.65%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAF--LRLLRGDFHR 2449
            + +KA RT++ IL AF++ WTP++I+VL+  +  D V    + L YWLCY NS +NP CYAL N  F+  F  L L R D  R
Sbjct:  359 KEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTVNPMCYALCNKAFRDTFRLLLLCRWDKRR 441
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Mouse
Match: Chrm1 (cholinergic receptor, muscarinic 1, CNS [Source:MGI Symbol;Acc:MGI:88396])

HSP 1 Score: 156.377 bits (394), Expect = 2.466e-40
Identity = 80/199 (40.20%), Postives = 111/199 (55.78%), Query Frame = 2 
Query:  440 AILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDA 1036
            A +G   G++SLATV GNLLV+ SF V   L+   NYF+ SLA  DL+IG FSMN YT YLL+ +W LG + CDLWL+LDY A   S   +  I+ DR+ SV  P  YR  RT  +  +M+   W++   ++   IL W +  G+R     +C+  F +         +  F+L + VMC+LY  IY    N  R+  A
Sbjct:   26 AFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAA 224

HSP 2 Score: 80.8777 bits (198), Expect = 2.228e-15
Identity = 38/83 (45.78%), Postives = 52/83 (62.65%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAF--LRLLRGDFHR 2449
            + +KA RT++ IL AF++ WTP++I+VL+  +  D V    + L YWLCY NS +NP CYAL N  F+  F  L L R D  R
Sbjct:  359 KEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTVNPMCYALCNKAFRDTFRLLLLCRWDKRR 441
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Mouse
Match: Chrm3 (cholinergic receptor, muscarinic 3, cardiac [Source:MGI Symbol;Acc:MGI:88398])

HSP 1 Score: 155.221 bits (391), Expect = 3.899e-39
Identity = 97/308 (31.49%), Postives = 149/308 (48.38%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDH----RKPTNNMVLDEMTPGEDNPKKSKGFH-WHGNKVDQSGSHQKLKQKKHSEKQD-DRSSSPTFPSDSDIVSIDN 1351
            + FL G ++L T+ GN+LVI +F V + L+   NYF+ SLA  DL+IG+ SMN +T Y+++N W LG + CDLWLS+DY A   S   +  I+ DR+ S+  P  YR  RT  +  +M+   W+I   ++   IL W +F GKR  P  EC+  F ++        I  F++ + +M  LY  IY        K   KR K  +            G+  +  E E E F H     +  ++  L +      + +K  G H W   K            K  +E+ D D SSS ++ ++    S++N
Sbjct:   70 IAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIY--------KETEKRTKELA------------GLQASGTEAEAENFVHPTGSSRSCSSYELQQQGTKRSSRRKYGGCHFWFTTK----------SWKPSAEQMDQDHSSSDSWNNNDAAASLEN 347

HSP 2 Score: 80.4925 bits (197), Expect = 3.997e-15
Identity = 56/186 (30.11%), Postives = 85/186 (45.70%), Query Frame = 2 
Query: 1919 MGTENFVQRNSTKAEGVKS------SHIQFCEIPNQTFQRHDIKTLNKTKRQS---------SYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            +GT + V+RN+ K +  KS          F ++P Q     D    + T             S++     + F L+ R        +IT R R    +E              +KA +T++ IL AF+I WTP++I+VL+  +    +   ++NL YWLCY NS +NP CYAL N  F+  F  LL
Sbjct:  395 LGTMD-VERNAHKLQAQKSMDDRDNCQKDFSKLPIQLESAVDTAKTSDTNSSVDKTTAALPLSFKEATLAKRFALKTRS-------QITKRKRMSLIKE--------------KKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLL 558
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Mouse
Match: Chrm3 (cholinergic receptor, muscarinic 3, cardiac [Source:MGI Symbol;Acc:MGI:88398])

HSP 1 Score: 155.221 bits (391), Expect = 3.899e-39
Identity = 97/308 (31.49%), Postives = 149/308 (48.38%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDH----RKPTNNMVLDEMTPGEDNPKKSKGFH-WHGNKVDQSGSHQKLKQKKHSEKQD-DRSSSPTFPSDSDIVSIDN 1351
            + FL G ++L T+ GN+LVI +F V + L+   NYF+ SLA  DL+IG+ SMN +T Y+++N W LG + CDLWLS+DY A   S   +  I+ DR+ S+  P  YR  RT  +  +M+   W+I   ++   IL W +F GKR  P  EC+  F ++        I  F++ + +M  LY  IY        K   KR K  +            G+  +  E E E F H     +  ++  L +      + +K  G H W   K            K  +E+ D D SSS ++ ++    S++N
Sbjct:   70 IAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIY--------KETEKRTKELA------------GLQASGTEAEAENFVHPTGSSRSCSSYELQQQGTKRSSRRKYGGCHFWFTTK----------SWKPSAEQMDQDHSSSDSWNNNDAAASLEN 347

HSP 2 Score: 80.4925 bits (197), Expect = 3.997e-15
Identity = 56/186 (30.11%), Postives = 85/186 (45.70%), Query Frame = 2 
Query: 1919 MGTENFVQRNSTKAEGVKS------SHIQFCEIPNQTFQRHDIKTLNKTKRQS---------SYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            +GT + V+RN+ K +  KS          F ++P Q     D    + T             S++     + F L+ R        +IT R R    +E              +KA +T++ IL AF+I WTP++I+VL+  +    +   ++NL YWLCY NS +NP CYAL N  F+  F  LL
Sbjct:  395 LGTMD-VERNAHKLQAQKSMDDRDNCQKDFSKLPIQLESAVDTAKTSDTNSSVDKTTAALPLSFKEATLAKRFALKTRS-------QITKRKRMSLIKE--------------KKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLL 558
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Mouse
Match: Chrm4 (cholinergic receptor, muscarinic 4 [Source:MGI Symbol;Acc:MGI:88399])

HSP 1 Score: 148.673 bits (374), Expect = 1.261e-37
Identity = 86/247 (34.82%), Postives = 128/247 (51.82%), Query Frame = 2 
Query:  362 LNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVAL------NLQRKSDAKRRKMTSLVSMAGQTM 1084
            +NG++   ++ L    H  L  + +  I   + G +SL TV GN+LV+ S  V R L+   NYF+ SLA  DL+IG FSMN YT+Y++  YWPLG + CDLWL+LDY     S   +  I+ DR+  V  P  Y   RT     +M+AA W++   ++   IL W F  GKR  P N+C+  F ++        I  F+L +++M  LY    H++L      +  R    K +K  +L  +    M
Sbjct:    7 VNGSSANQSVRLVTTAHNHLETVEMVFI-ATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTLYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLY---IHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLM 249

HSP 2 Score: 82.8037 bits (203), Expect = 5.686e-16
Identity = 37/90 (41.11%), Postives = 55/90 (61.11%), Query Frame = 2 
Query: 2177 EIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            ++ ++ +   R RK  RTI  IL AF++ WTP++++VL+  +    +    +++ YWLCY NS INP CYAL NA FKK F  LL   + 
Sbjct:  384 QVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPERVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYR 473
[Close]
BLAST of Muscarinic acetylcholine receptor vs. UniProt/SwissProt
Match: sp|Q09388|ACM2_CAEEL (Muscarinic acetylcholine receptor gar-2 OS=Caenorhabditis elegans OX=6239 GN=gar-2 PE=2 SV=3)

HSP 1 Score: 194.512 bits (493), Expect = 2.303e-51
Identity = 96/214 (44.86%), Postives = 133/214 (62.15%), Query Frame = 2 
Query:  434 VTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYT-IYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGK-RGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSM 1069
             + +L      +S+ TV GNL V+ S+++++ +R PTNYFI SLA++DLLIG+  +  YT  YL  N W  G++ CDLWLS+DY  CL S YTV  ITVDR+ SV  PA YRNWRT  +V++++   W++PS +F   I G+  FTG  R   + EC+  F T+ + N  + I Y+W TL VM  LY GIY  A  L  KSD K +++  L  M
Sbjct:    4 ASVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIPVYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYWTTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEM 217

HSP 2 Score: 113.62 bits (283), Expect = 1.046e-24
Identity = 51/91 (56.04%), Postives = 65/91 (71.43%), Query Frame = 2 
Query: 2189 RGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFN----LSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            + R+ENRARKALRTIT ILG+F+I WTPF++L  + G+         FN    +SY+LCY NSP+NPFCYA+AN QFKK   R+ +GDF R
Sbjct:  536 KSRSENRARKALRTITFILGSFIILWTPFYVLATIYGFCETCKASPSFNTLYTISYYLCYMNSPLNPFCYAMANQQFKKTLTRIFKGDFRR 626
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BLAST of Muscarinic acetylcholine receptor vs. UniProt/SwissProt
Match: sp|Q18007|ACM1_CAEEL (Probable muscarinic acetylcholine receptor gar-1 OS=Caenorhabditis elegans OX=6239 GN=gar-1 PE=2 SV=3)

HSP 1 Score: 167.548 bits (423), Expect = 8.565e-42
Identity = 82/216 (37.96%), Postives = 133/216 (61.57%), Query Frame = 2 
Query:  467 VSLATVAGNLLVITSFFVERAL-RNPTNYFIASLAVTDLLIGIFSMNFYTIYLL-LNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKR-GCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGM 1105
            +SL T+ GN +V+ ++ +ER + +  +N +I SLA++DL+IGI    F+T+Y+L  + WPLG + C  WL LDYT CL S  TV  IT DR+ SV   AKY  W++  K  +++  +W++P+ IF   I GW   TG+       EC A F ++ + N  + + Y+W TL+ M  LY GI+  A NL++K+ AK R+  +L+ ++ +  +++GV +
Sbjct:   34 LSLETIIGNAMVVMAYRIERNISKQVSNRYIVSLAISDLIIGIEGFPFFTVYVLNGDRWPLGWVACQTWLFLDYTLCLVSILTVLLITADRYLSVCHTAKYLKWQSPTKTQLLIVMSWLLPAIIFGIMIYGWQAMTGQSTSMSGAECSAPFLSNPYVNMGMYVAYYWTTLVAMLILYKGIHQAAKNLEKKAKAKERRHIALI-LSQRLGTQVGVSL 248

HSP 2 Score: 99.3673 bits (246), Expect = 4.487e-20
Identity = 45/98 (45.92%), Postives = 64/98 (65.31%), Query Frame = 2 
Query: 2171 PKEIFERGR----TENRARKALRTITLILGAFVICWTPFHILVLLKGY-YPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            P  +F RGR     E RA KA RTIT I+G F I W+P++I+  + G+   + +    + LSY++CY NS  NPF YALAN QF+ AF+R+ RG+F++
Sbjct:  614 PIAVFNRGRKQTKAEKRAHKAFRTITFIVGFFAILWSPYYIMATVYGFCKGECIPSFLYTLSYYMCYLNSSGNPFAYALANRQFRSAFMRMFRGNFNK 711
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BLAST of Muscarinic acetylcholine receptor vs. UniProt/SwissProt
Match: sp|Q5R949|ACM1_PONAB (Muscarinic acetylcholine receptor M1 OS=Pongo abelii OX=9601 GN=CHRM1 PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 9.241e-40
Identity = 80/207 (38.65%), Postives = 118/207 (57.00%), Query Frame = 2 
Query:  440 AILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSL 1060
            A +G   G++SLATV GNLLV+ SF V   L+   NYF+ SLA  DL+IG FSMN YT YLL+ +W LG + CDLWL+LDY A   S   +  I+ DR+ SV  P  YR  RT  +  +M+   W++   ++   IL W +  G+R     +C+  F +         +  F+L + VMC+LY  IY       R+++++ R++ +L
Sbjct:   26 AFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIY-------RETESRARELAAL 225

HSP 2 Score: 82.4185 bits (202), Expect = 5.106e-15
Identity = 46/108 (42.59%), Postives = 64/108 (59.26%), Query Frame = 2 
Query: 2144 RTRAKPCREP--KEIFERGRTEN--RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAF--LRLLRGDFHR 2449
            R RA   ++P  KE   + +T +  + +KA RT++ IL AF++ WTP++I+VL+  +  D V    + L YWLCY NS INP CYAL N  F+  F  L L R D  R
Sbjct:  334 RDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRR 441
[Close]
BLAST of Muscarinic acetylcholine receptor vs. UniProt/SwissProt
Match: sp|P56489|ACM1_MACMU (Muscarinic acetylcholine receptor M1 OS=Macaca mulatta OX=9544 GN=CHRM1 PE=2 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 1.085e-39
Identity = 80/199 (40.20%), Postives = 111/199 (55.78%), Query Frame = 2 
Query:  440 AILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDA 1036
            A +G   G++SLATV GNLLV+ SF V   L+   NYF+ SLA  DL+IG FSMN YT YLL+ +W LG + CDLWL+LDY A   S   +  I+ DR+ SV  P  YR  RT  +  +M+   W++   ++   IL W +  G+R     +C+  F +         +  F+L + VMC+LY  IY    N  R+  A
Sbjct:   26 AFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAA 224

HSP 2 Score: 82.8037 bits (203), Expect = 3.811e-15
Identity = 46/108 (42.59%), Postives = 64/108 (59.26%), Query Frame = 2 
Query: 2144 RTRAKPCREP--KEIFERGRTEN--RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAF--LRLLRGDFHR 2449
            R RA   ++P  KE   + +T +  + +KA RT++ IL AF++ WTP++I+VL+  +  D V    + L YWLCY NS INP CYAL N  F+  F  L L R D  R
Sbjct:  334 RDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRR 441
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BLAST of Muscarinic acetylcholine receptor vs. UniProt/SwissProt
Match: sp|P12657|ACM1_MOUSE (Muscarinic acetylcholine receptor M1 OS=Mus musculus OX=10090 GN=Chrm1 PE=1 SV=2)

HSP 1 Score: 156.377 bits (394), Expect = 1.725e-39
Identity = 80/199 (40.20%), Postives = 111/199 (55.78%), Query Frame = 2 
Query:  440 AILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDA 1036
            A +G   G++SLATV GNLLV+ SF V   L+   NYF+ SLA  DL+IG FSMN YT YLL+ +W LG + CDLWL+LDY A   S   +  I+ DR+ SV  P  YR  RT  +  +M+   W++   ++   IL W +  G+R     +C+  F +         +  F+L + VMC+LY  IY    N  R+  A
Sbjct:   26 AFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAA 224

HSP 2 Score: 80.8777 bits (198), Expect = 1.559e-14
Identity = 38/83 (45.78%), Postives = 52/83 (62.65%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAF--LRLLRGDFHR 2449
            + +KA RT++ IL AF++ WTP++I+VL+  +  D V    + L YWLCY NS +NP CYAL N  F+  F  L L R D  R
Sbjct:  359 KEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTVNPMCYALCNKAFRDTFRLLLLCRWDKRR 441
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BLAST of Muscarinic acetylcholine receptor vs. TrEMBL
Match: A0A193KU58 (GCR010 OS=Schmidtea mediterranea OX=79327 GN=gcr010 PE=2 SV=1)

HSP 1 Score: 1433.7 bits (3710), Expect = 0.000e+0
Identity = 704/704 (100.00%), Postives = 704/704 (100.00%), Query Frame = 2 
Query:  341 MSVSSGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWXXXXXXXXXXXXXXXDQGINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGFCPSTENNSPVWIKRKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            MSVSSGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWRHNNSYHHSNNRHSSDQGINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGFCPSTENNSPVWIKRKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT
Sbjct:    1 MSVSSGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWRHNNSYHHSNNRHSSDQGINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGFCPSTENNSPVWIKRKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 704
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BLAST of Muscarinic acetylcholine receptor vs. TrEMBL
Match: A0A1S3HMP0 (muscarinic acetylcholine receptor M4 OS=Lingula unguis OX=7574 GN=LOC106155855 PE=3 SV=1)

HSP 1 Score: 435.261 bits (1118), Expect = 1.265e-137
Identity = 266/697 (38.16%), Postives = 365/697 (52.37%), Query Frame = 2 
Query:  413 TLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNY-WPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSE--KQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMST---VTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWXXXXXXXXXXXXXXXDQGINKSNTTATGLQYIDSNSLQNSRS----KLILNISPANGFCPST-ENNSPVWIKRKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKS------SHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            TL  P+ +T +L    G+VS+ TV GN LV+ SF+++R +R P+NYFIASLAV+DLLIG+FSM  YT+YLLL+  WPLG   CDLWLSLDYT+CL SQYTVFFITVDRFCSV IPAKYRNWRT++KV +MV+ TW+IP  IF+T I+GW +F G R  P  ECW  F +   FNTIL IGY+W+TL+VM  LY GIY VAL+L RKS AK++KMTSLVSMAGQTM+ IG+G++           +K +   V + ++P                          LK KK +   ++++ SSS  +PSD +  S  +    P   +++        + K  Q                 +   + N  T   +TT    ++            S   N+S  QTA    T   S                      ++     G+++ID  S Q+  S    KL++     +    S  E  SP+W KR+   +  + E   +       ++D +  +  V   S     +K+      +H         T Q HDI T         Y RR N                     + + K   + K    + ++ENRARKALRTI+LILGAFV+ WTP+H++V++ G+  D V++  + +SYW CY NSPINPFCYA ANAQFK+ F R+L GD HRT
Sbjct:  103 TLRHPMWLTVLLAIFGGIVSIVTVLGNSLVLLSFYLDRQIRQPSNYFIASLAVSDLLIGLFSMPLYTMYLLLDAKWPLGPTVCDLWLSLDYTSCLASQYTVFFITVDRFCSVKIPAKYRNWRTEHKVNVMVSVTWVIPILIFYTSIIGWQYFVGLRTVPAQECWVQFMSSPMFNTILVIGYYWITLVVMIILYAGIYKVALDLHRKSAAKQKKMTSLVSMAGQTMTNIGIGISQT---------KKASVKEVKETISP--------------------------LKNKKATAAVEEEEGSSSTAYPSDVEDSSSQSAPHPPPAYNSLDGDSAGTPVNKPKQHHHHHHHRHHHHHHGKDHAHGHKNKKTDDHLTTPKGEQYLTTPTPVSQTPSSLLDNESSTQTAGSHDTSGSS---------------------GETPKRGHGIRFIDQESFQSFASGEDVKLVVETRHIDKLAQSNDEPPSPIW-KRRQSFKAKETEHSAANMVGCNGDKDKVCMQVSVNSKSKGKPVIKTIKPPSGNHEHKSARERVTQQWHDITT---------YFRRKN--------------------QKRKEKGKAQGK---NKSKSENRARKALRTISLILGAFVLFWTPYHVIVIILGFCYDCVHIRLYEISYWFCYMNSPINPFCYAFANAQFKRTFKRILSGDLHRT 710
[Close]
BLAST of Muscarinic acetylcholine receptor vs. TrEMBL
Match: K1QZ79 (Putative muscarinic acetylcholine receptor gar-2 OS=Crassostrea gigas OX=29159 GN=CGI_10014948 PE=3 SV=1)

HSP 1 Score: 397.512 bits (1020), Expect = 1.108e-123
Identity = 254/687 (36.97%), Postives = 366/687 (53.28%), Query Frame = 2 
Query:  434 VTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPG-EDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKK---IQSPVILDDSPSPSETSPFNF-FQYGNMSTVTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWXXXXXXXXXXXXXXXDQGINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGF---CPSTENNSPVWIKRK---VDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYP---DLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            +T ILG L   + + TVAGN+LV+ SF +ER +R PTNYFIASLAV+DLLIG FSM  +T+YLLL YWPLGE+ CDLWL+LD+T CL SQYTVFFITVDRF SV IPAKYR+WRT+ KV+ MV+ TWI P+ IFFT I+GW +F GKR     +C   F +D  F  +LTIGY+W+ L VM  LYGGIY+VAL LQ K++AK +KMT+ + ++    S                  +   N    D  T G E+N  +S        + D+S S  K         +++RSSSP F SD +    +N  QG   +        D+   ++   + S V  D +  PS        F + +   +T T +S           G+ +    + D         E    + H     +      +D+ +   N    G +YI+ +SL+   S   L I P         + E+ SPVW +R+   + + +++D  +T      G       + +        + G  + H +          R++ + ++      + R + ++ A    +++++Q +  +   R ++K  R+        +TENRARKALRTIT ILGA+++CWTP+HI+VL+ G      + VN+  +  +YWLCY NSPINPFCYALAN QFKK FLR+LR D H+T
Sbjct:   45 LTIILGVLVAFIVVVTVAGNVLVLLSFALERTIRQPTNYFIASLAVSDLLIGSFSMPCFTLYLLLGYWPLGELVCDLWLALDWTVCLASQYTVFFITVDRFFSVKIPAKYRHWRTEKKVVTMVSLTWIFPTLIFFTSIVGWQYFVGKRTVKPLQCEVQFMSDPLFTFLLTIGYYWIPLCVMIGLYGGIYYVALTLQWKAEAKHKKMTTAMELSKNKQS-----------------SKSNRNASGADNNTLGTENNNGRSM-------RPDESTSFTK-------APEEERSSSPVFESDEE----NNSSQGACGSSKTHFRHADVDDDEQTCFVNSAVQTDTTYRPSLKGMLGMSFNHVSKIALTITGTS-----------GSETETDGNEDTNATKALIKEPPPPYNHVVPNTYEKASKQTDKLLE--NDVLEGCKYIEEDSLKTLTSNDNLKIYPDGSISFDATTDESCSPVWKRREGRYLPIPIAEDSSNTISTTLEGDSSIRHASNDDEMSKRIPSNGTSAVHSR---------DRNEDEDVD----SDANREKPSIMA---PLQNLVQNMRAK---RKKSKRTRQKV----NSKTENRARKALRTITFILGAYILCWTPYHIMVLIIGVCDGGWNCVNMKLYQFTYWLCYLNSPINPFCYALANQQFKKTFLRILRLDCHKT 660
[Close]
BLAST of Muscarinic acetylcholine receptor vs. TrEMBL
Match: A0A210QWZ4 (Muscarinic acetylcholine receptor gar-2 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT04597 PE=3 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 4.446e-120
Identity = 270/698 (38.68%), Postives = 384/698 (55.01%), Query Frame = 2 
Query:  422 FPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHW-HGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQ-----SPVILDDSPSPSETSPFNFFQYGNMSTV--TTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYV---SEWXXXXXXXXXXXXXXXDQGINKSNTTATGLQYIDSNSLQNSRSK---LILNISPANGFCPSTENNSPVWIKRKVD--LQVSDDEKDTSVENNNGHEQDPMGTE-NFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILV----LLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            +P+ +T +LG +A VV L TV GN+LV+ +F VER++R PTNYFIASLAV+DLLIG FSM  +T+YLLL YWPLG++ CDLWLSLD+T CL SQYTVFFITVDRF SV IPAKYRNWRT+ KV+IM+A TWIIP ++FFT I+GW +F G+R   + EC   F +D  F  +LTIGY+W TL VM  LY GIY VAL+LQ+K++AK++KMT+  +M+ Q+                   H K   +  LD     +++ K+++  +  H   VD +   +          ++DRSSSP F SD D  S    Q+G   +    LT +        Q     S V  D +  PS         +G++S +  + T+SS     + N++  A               + T  +   + +    +  +S N  + D  IN       G +YID +SL++  S     +L+    N    + E +SP+W KR+ D  + +   E   S       E   M T+ + +  N+  + G  S   +    P  + + +D    ++ K  S +       A    +R+  Q+       R +A   RE K    + ++ENRARKA RTIT ILGA+V+CWTP+H++V    L  GY  D +N+  +  +YWLCY NSP+NPFCYALAN QFK+ FLR++R DFHRT
Sbjct:   51 YPMWLTILLGAMAAVVVLVTVLGNILVLIAFAVERSIRQPTNYFIASLAVSDLLIGTFSMPCFTLYLLLGYWPLGDIICDLWLSLDWTVCLASQYTVFFITVDRFFSVKIPAKYRNWRTEKKVIIMIAFTWIIPCAVFFTSIIGWQYFVGERTVDKYECEVQFMSDPLFTFLLTIGYYWTTLFVMIGLYTGIYRVALSLQKKAEAKQKKMTT--AMSHQS-------------------HDKKKKSRALDAGGASQNSRKRTENNNARHLRGVDTTSFTKP--------PEEDRSSSPAFASDEDNSSS---QEGACGSSKTPLTGKHEDDDDDDQTCFVNSAVQTDTTYRPSLKGMLG-VSFGHVSKLAFSITASS---VTLPNDHGEAEEELHTPLPDALPPAYDTVVIPPDNNYAKKVNNSNSINELAVDDVIN-------GCKYIDEDSLKSLASAENIKLLSDPGTNNDSSADEGSSPIW-KRRADRYMPIPAPEGSNSTNMEVIEEDKTMDTDLDHIAPNTLPSNGKASVSGKPHRKPLDSVRSNDTSGSSERKFGSPF------HALVKSMREKQQK-------RQKAARQREQK----KSKSENRARKAFRTITFILGAYVLCWTPYHVMVFIIGLCGGY--DCINIKLYQFTYWLCYLNSPVNPFCYALANQQFKRTFLRIMRFDFHRT 685
[Close]
BLAST of Muscarinic acetylcholine receptor vs. TrEMBL
Match: A0A193KUP4 (GCR071 OS=Schmidtea mediterranea OX=79327 GN=gcr071 PE=2 SV=1)

HSP 1 Score: 324.709 bits (831), Expect = 8.529e-99
Identity = 167/323 (51.70%), Postives = 221/323 (68.42%), Query Frame = 2 
Query:  422 FPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGK-RGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTM---SRIGVGMTND---------------------ENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQK---KHSEKQDDRSS 1306
            FP  V+  LGFL+ VVS++T+ GNLLVI SFF ERALR  TNYFIASLAVTDLLIG+FSMNFYT+YLLL+YWPLGE+FCDLWLSLDYTACLTSQYTVFFITVDRFCSV IPAKYRNWRT++KV +MVA TWI+P ++FFT I+GWP+F  + R    ++C+A F  +  FNTILTI YFW+TL+VM  LY GIY VALNLQ KSD K+++++ L+  + + M   S  G+  +N                      +N   I+  RK  N+   ++++  +++PK++     + N  ++  S      K   ++  K+ D+S+
Sbjct:   18 FPPAVSWTLGFLSAVVSVSTIMGNLLVIVSFFFERALRTSTNYFIASLAVTDLLIGVFSMNFYTVYLLLSYWPLGELFCDLWLSLDYTACLTSQYTVFFITVDRFCSVKIPAKYRNWRTESKVRLMVAITWIVPFAVFFTTIMGWPYFNNQGRIREPDQCYAEFAKEPIFNTILTIAYFWITLMVMVILYAGIYKVALNLQAKSDHKKKRISELIPSSSKRMTETSESGIRTSNASQSLSNSIKQSSYHLGVIQMLDNTSSIWVRRKIENDFTHNKVS-SQNSPKQTNNEILYKNNANKRSSSLIAPIKFVAQYFYKKSDKST 339

HSP 2 Score: 135.191 bits (339), Expect = 4.213e-30
Identity = 63/102 (61.76%), Postives = 75/102 (73.53%), Query Frame = 2 
Query: 2165 REPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPD------LVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            +  K    R RT +RARKALRTIT ILGAFV+ WTPFHI+VL+KG+          VN H +NL+YWLCY NSPINPFCYALAN QFK+ FLR+L+ DF R+
Sbjct:  334 KSDKSTKRRERTVSRARKALRTITFILGAFVLSWTPFHIIVLIKGFCDSTKDKHTCVNSHLYNLTYWLCYLNSPINPFCYALANVQFKRTFLRILKFDFRRS 435
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Cavefish
Match: chrm2a (cholinergic receptor muscarinic 2 [Source:NCBI gene;Acc:103039136])

HSP 1 Score: 152.91 bits (385), Expect = 5.201e-39
Identity = 85/246 (34.55%), Postives = 126/246 (51.22%), Query Frame = 2 
Query:  452 FLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGE 1189
             +AG +SL TV GN+LV+ S  V R+L+   NYF+ SLA  DL+IG+ SMN YT+Y+++ YWPLG + CDLWL+LDY     S   +  I+ DR+  V  P  Y   RT     +M+AA W++   ++   IL W F  G R  P+ EC+  F ++        I  F+L +I+M  LY  I   + +  +K + K               S   VG  +      I   +   NNMV+++   G+
Sbjct:   38 LVAGSLSLVTVIGNILVMLSIKVNRSLQTVNNYFLFSLACADLIIGLCSMNLYTVYIVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLSYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVPEQECYIQFFSNAAVTFGTAIAAFYLPVIIMMVLYWQISRASKSRVKKDNRKP--------------SGANVGPASPSQTRSIMASKPDNNNMVVEDPDRGQ 269

HSP 2 Score: 81.2629 bits (199), Expect = 1.296e-15
Identity = 37/76 (48.68%), Postives = 48/76 (63.16%), Query Frame = 2 
Query: 2204 NRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            +R +K  RTI  IL AFV  WTP++++VL+  +    +    + + YWLCY NS INP CYAL NA FKK F  LL
Sbjct:  409 SREKKVTRTIMAILVAFVATWTPYNVMVLINTFCSSCIPNTVWTIGYWLCYINSTINPACYALCNATFKKTFKHLL 484
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Cavefish
Match: chrm2b (muscarinic acetylcholine receptor M2-like [Source:NCBI gene;Acc:103021745])

HSP 1 Score: 149.828 bits (377), Expect = 2.784e-38
Identity = 85/218 (38.99%), Postives = 120/218 (55.05%), Query Frame = 2 
Query:  407 NHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIG----YFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRK 1048
            N T   P  V  ILG  +  +SL T+ GN+LV+ S  V + L+   NYF+ SLA  DL+IG+FSMN YT+Y++  YWPLG + CDLWL+LDY A   S   +  I+ DR+  V  P  Y   RT     +M+A  WI+   ++   IL W FF G+R   + EC+  F    F N ++T G     F+L +++M  LY  I   +     +S  KRR+
Sbjct:   16 NETFTLPQKVLIILGLSS--LSLITIIGNVLVMVSIKVNKNLQMVNNYFLFSLACADLIIGLFSMNLYTVYIVTGYWPLGPVVCDLWLALDYVASNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGVMIAVAWILSFILWAPAILFWQFFVGERTVEKRECYIQF----FSNPVVTFGTAIAAFYLPVVIMSVLYWQISKAS-----RSRIKRRE 222

HSP 2 Score: 83.9593 bits (206), Expect = 1.848e-16
Identity = 38/92 (41.30%), Postives = 52/92 (56.52%), Query Frame = 2 
Query: 2171 PKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            P    +R R  +R RK  RTI  IL AFV  WTP++++VL+  +    +    + + YWLCY NS +NP CYAL N  FK  F +LL   + 
Sbjct:  362 PPHKKKRNRCSSRERKVTRTIMAILVAFVATWTPYNVMVLISTFCRSCIPNSMWAMGYWLCYINSTVNPACYALCNVTFKNTFKQLLMCKYK 453
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Cavefish
Match: chrm2b (muscarinic acetylcholine receptor M2-like [Source:NCBI gene;Acc:103021745])

HSP 1 Score: 149.828 bits (377), Expect = 2.784e-38
Identity = 85/218 (38.99%), Postives = 120/218 (55.05%), Query Frame = 2 
Query:  407 NHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIG----YFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRK 1048
            N T   P  V  ILG  +  +SL T+ GN+LV+ S  V + L+   NYF+ SLA  DL+IG+FSMN YT+Y++  YWPLG + CDLWL+LDY A   S   +  I+ DR+  V  P  Y   RT     +M+A  WI+   ++   IL W FF G+R   + EC+  F    F N ++T G     F+L +++M  LY  I   +     +S  KRR+
Sbjct:   16 NETFTLPQKVLIILGLSS--LSLITIIGNVLVMVSIKVNKNLQMVNNYFLFSLACADLIIGLFSMNLYTVYIVTGYWPLGPVVCDLWLALDYVASNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGVMIAVAWILSFILWAPAILFWQFFVGERTVEKRECYIQF----FSNPVVTFGTAIAAFYLPVVIMSVLYWQISKAS-----RSRIKRRE 222

HSP 2 Score: 83.9593 bits (206), Expect = 1.848e-16
Identity = 38/92 (41.30%), Postives = 52/92 (56.52%), Query Frame = 2 
Query: 2171 PKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            P    +R R  +R RK  RTI  IL AFV  WTP++++VL+  +    +    + + YWLCY NS +NP CYAL N  FK  F +LL   + 
Sbjct:  362 PPHKKKRNRCSSRERKVTRTIMAILVAFVATWTPYNVMVLISTFCRSCIPNSMWAMGYWLCYINSTVNPACYALCNVTFKNTFKQLLMCKYK 453
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Cavefish
Match: chrm5a (muscarinic acetylcholine receptor M5 [Source:NCBI gene;Acc:103025843])

HSP 1 Score: 149.058 bits (375), Expect = 1.621e-37
Identity = 73/185 (39.46%), Postives = 107/185 (57.84%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIY 1000
            +  ++ +VSL T+ GN+LV+ SF V   L+   NY++ SLA  DL+IG+FSMN YT Y+L+ YW LG + CDLWL+LDY A   S   +  I+ DR+ S+  P  YR  RT  +  IM+   W++   ++   IL W +F GKR  P+ +C   F ++        I  F++ + VM  LY  IY
Sbjct:   31 IATVSAIVSLITIIGNVLVMVSFKVNSQLKTVNNYYLLSLAFADLIIGVFSMNLYTSYILMGYWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPRRAGIMIGLAWLVSFVLWAPPILCWQYFVGKRTVPERQCQIQFFSEPVITFGTAIAAFYIPVSVMTILYCRIY 215

HSP 2 Score: 85.8853 bits (211), Expect = 5.740e-17
Identity = 35/75 (46.67%), Postives = 52/75 (69.33%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            + RKA +T++ IL AF++ WTP++I+VL+  +  D + +  ++L YWLCY NS +NP CYAL N  F+K F  LL
Sbjct:  421 KERKAAQTLSAILLAFILTWTPYNIMVLISTFCSDCIPLSLWHLGYWLCYVNSTVNPMCYALCNKNFQKTFRMLL 495
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Cavefish
Match: chrm4a (cholinergic receptor muscarinic 4 [Source:NCBI gene;Acc:103043196])

HSP 1 Score: 146.747 bits (369), Expect = 8.333e-37
Identity = 76/206 (36.89%), Postives = 113/206 (54.85%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALN-----LQRKSDAKRRK 1048
            +  + G +S  TV GN+LV+ S  V R L+   NYF+ SLA  DL+IG+FSMN YT+Y++  YWPLG + CDLWL+LDY     S   +  I+ DR+  V  P  Y   RT     +M+A+ WI+   ++   IL W F  G+R     EC+  F ++        I  F+L +++M  LY    H++L       ++K +AK+ K
Sbjct:   62 IALVTGSLSFVTVVGNILVMLSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTVYIIKGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLSYPTRRTTKMAGLMIASAWILSFILWAPAILFWQFIVGERTVEPGECYIQFLSNPAVTFGTAIAAFYLPVVIMTVLY---IHISLASRSRVSKQKPEAKKEK 264

HSP 2 Score: 80.4925 bits (197), Expect = 2.582e-15
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 2 
Query: 2177 EIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            ++  + +   R +K  +TI  IL AF+I WTP++++VL+  +    V    + + YWLCY NS INP CYAL NA FKK F  LL   + 
Sbjct:  421 QVKRKRQMAAREKKVTKTIFAILLAFIITWTPYNVMVLISTFCHSCVPDTVWAIGYWLCYVNSTINPACYALCNATFKKTFKNLLMCQYK 510
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Sea Lamprey
Match: CHRM3 (cholinergic receptor muscarinic 3 [Source:HGNC Symbol;Acc:HGNC:1952])

HSP 1 Score: 160.229 bits (404), Expect = 5.016e-42
Identity = 102/329 (31.00%), Postives = 159/329 (48.33%), Query Frame = 2 
Query:  443 ILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRI------------GVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLT 1393
            ++G L G+VSL T+ GN+LV+ +F V R L+   NYF+ SLAV DL+IG+ SMN YT Y++++ W LG+  CDLWL +DY A   S   +  I+ DR+ S+  P KY+  RT  +V +M+   W++   ++   IL W +  GKR  P NEC+  F +         I  F+L + +M  LY  IY   L  Q+++    R++  L     ++ SRI              G   D +  +    ++P+   +L          K  K          + G  +  K   +S ++ D S   +  SD D    D   Q PK   +++LT
Sbjct:   14 VIGTLTGIVSLLTIVGNILVMVAFKVNRQLKTVNNYFLLSLAVADLIIGVVSMNLYTTYIIMSRWALGQAACDLWLVVDYVASNASVMNLLVISFDRYLSITRPLKYKAKRTTKRVALMIGLAWVVSLVLWGPAILFWQYVDGKRTVPDNECFIQFLSVPAITFGTAIAAFYLPVTIMMVLYWRIY---LETQKRT----RELAGLQGSQFESSSRILTQPGSSRQSSTAEGTATDADPAQPVQVKRPSKYGLLKHKWATSSRVKSIK---------SRDGRDRARKVNWNSVREGDPSPIHSLCSDED---DDPPPQRPKAIFSIVLT 323

HSP 2 Score: 52.7582 bits (125), Expect = 2.307e-7
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 2 
Query: 2282 LVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            L+L+  +  + +   ++ L YWLCY NS +NP CYAL N  F+K F  +L   + R
Sbjct:  438 LILVSTFCDNCIPKVWWRLGYWLCYVNSTVNPMCYALCNKTFRKTFKWILLCQWER 493
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Sea Lamprey
Match: CHRM3 (cholinergic receptor muscarinic 3 [Source:HGNC Symbol;Acc:HGNC:1952])

HSP 1 Score: 156.762 bits (395), Expect = 1.042e-40
Identity = 73/193 (37.82%), Postives = 112/193 (58.03%), Query Frame = 2 
Query:  446 LGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQR 1024
            +G   GV+SLAT+ GN+LV+ SF V + L    NY++ SLAV DL+IGIFSMN +T Y+++ +W LG + CDLWL+LDY A   S   +  I+ DRF S+  P  YR  RT ++  +++   W++   ++   IL W +  G+R  P +EC   F ++        I  F+L + +M ++Y  I+    N  R
Sbjct:   63 IGLFTGVMSLATIVGNVLVLVSFKVNKQLHTVNNYYLLSLAVADLIIGIFSMNLFTCYMIMGHWALGSLACDLWLALDYVASNASVMNLLVISFDRFFSITRPLTYRAKRTPHRAALLIGLAWVVSFVLWAPAILFWQYIVGERTVPPDECRIQFLSEPIITFCTAIAAFYLPVTIMATIYWRIFKEIQNRAR 255

HSP 2 Score: 87.4261 bits (215), Expect = 4.007e-18
Identity = 37/75 (49.33%), Postives = 53/75 (70.67%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            + +KA RT++ IL AF+I WTP++I+VL+  + P+ V    +N+ YWLCY NS +NP CYAL N  F+K F +LL
Sbjct:  466 KEKKAARTLSAILLAFIITWTPYNIMVLMSTFCPECVPPVLWNIGYWLCYVNSTVNPVCYALCNRIFRKTFRQLL 540
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Sea Lamprey
Match: chrm5b (cholinergic receptor, muscarinic 5b [Source:ZFIN;Acc:ZDB-GENE-041001-169])

HSP 1 Score: 148.673 bits (374), Expect = 3.641e-38
Identity = 77/211 (36.49%), Postives = 114/211 (54.03%), Query Frame = 2 
Query:  470 SLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVG 1102
            SL TV GN LV+ SF V R L+  +NYF+ SLA  DL++G+FSMN YT Y+L   W +G + CDLWL+LDY A   S   +  I+ DR+ S+  P KY+  RT  +  IM+   W +   ++   IL W    GKR  PQ++C+  F ++        I  F+L + +M  LY  IY       R++  + R+  +L     ++ S +  G
Sbjct:   41 SLITVVGNTLVMLSFRVSRQLKTVSNYFLLSLAAADLIVGLFSMNLYTAYILTGRWAMGAVMCDLWLTLDYVASNASVMNLLAISFDRYFSITRPLKYKAKRTKRRASIMIGLAWAVSLVLWAPAILFWQHMVGKRTVPQDQCYIQFLSEPIITFGTAIAAFYLPITIMMVLYWRIY-------RETRRRSRQFATLQGSESKSRSHVPTG 244

HSP 2 Score: 82.0333 bits (201), Expect = 2.016e-16
Identity = 35/75 (46.67%), Postives = 49/75 (65.33%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            R  KA+R +  IL AF++ WTP++I+VL+  +  D V    ++L YWLCY NS +NP CYAL N  F+K F  +L
Sbjct:  442 RDNKAVRILCAILLAFIVTWTPYNIMVLVSTFCDDCVPKKLWHLGYWLCYVNSTVNPMCYALCNMHFRKTFKTIL 516
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Sea Lamprey
Match: chrm4a (cholinergic receptor, muscarinic 4a [Source:ZFIN;Acc:ZDB-GENE-090410-6])

HSP 1 Score: 120.168 bits (300), Expect = 5.253e-29
Identity = 76/186 (40.86%), Postives = 101/186 (54.30%), Query Frame = 2 
Query:  431 PVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLY 988
            P +A++    G +SL TV GN LVI S  V R L+   +YFI SLA  DL+IG FSMN Y +Y+    WPLG   CDLWLS+DY A   S   +  I+ DRF  V  P  Y   RT     I++AA W++   ++   I+ W F  G R  PQ+ C+  F ++        I  F+L + VMC LY
Sbjct:   32 PRSALIFLGTGALSLLTVLGNALVIISICVNRRLQTVNSYFIFSLACADLIIGAFSMNVYAVYVWRGAWPLGAALCDLWLSIDYVASNASVMNLLIISCDRFLCVSRPHTYPARRTARAAAIVIAAAWLLSLLLWAPAIVLWQFVEGVRTVPQDACYIQFFSNPAATLGTAIAAFYLPVAVMCILY 217

HSP 2 Score: 84.3445 bits (207), Expect = 3.076e-17
Identity = 37/81 (45.68%), Postives = 52/81 (64.20%), Query Frame = 2 
Query: 2192 GRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLR 2434
            G    R RK  RT+  +L AF++ WTP++++VLL  +  D V    + L YWLCY NS +NP CYAL   +FK+ FL+L+R
Sbjct:  380 GARARRERKLTRTVAAVLLAFIVTWTPYNVMVLLAVFCTDCVPHAAWELGYWLCYVNSAVNPACYALCIREFKRTFLQLMR 460
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Sea Lamprey
Match: HTR1F (5-hydroxytryptamine receptor 1F [Source:HGNC Symbol;Acc:HGNC:5292])

HSP 1 Score: 118.627 bits (296), Expect = 1.022e-28
Identity = 66/190 (34.74%), Postives = 97/190 (51.05%), Query Frame = 2 
Query:  491 NLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSL 1060
            N LVI +    R LR P NY I SLAV DLL+    M     Y++   W LG   CDLWLSLD T C  S   +  I +DRF ++   A+Y + RT  +  +M+AA+W++ +     CI   P F  +R   +  C       V +    T G F++ L ++ +LY  I+  A  L  K ++K+R+  S+
Sbjct:   65 NALVIAAISATRKLRQPANYLIGSLAVADLLVAALVMPVAAAYVVTGEWALGGALCDLWLSLDVTCCTASILHLCAIALDRFWAITDAAEYAHKRTPGRAALMIAASWLLAA-----CISVPPLFWRRRDPAKPRCIIE-HDHVLYTIYSTFGAFYVPLALLLALYCRIFRAAKRLYEKRESKQREKLSV 248

HSP 2 Score: 62.7734 bits (151), Expect = 1.619e-10
Identity = 34/77 (44.16%), Postives = 45/77 (58.44%), Query Frame = 2 
Query: 2204 NRARKALRTITLILGAFVICWTPFHILVLLKGYYPDL-VNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            +R R+A +T+ LILGAFV+CW PF I  L+        V+    +L  WL Y NS INP  Y + N  FK AF +L+
Sbjct:  345 SRERRAAKTLGLILGAFVVCWLPFFIKELVVNLCKSCAVSPEVADLLTWLGYMNSLINPLIYTMFNEDFKSAFRKLV 421
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Nematostella
Match: EDO47359 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMJ9])

HSP 1 Score: 100.138 bits (248), Expect = 1.730e-22
Identity = 72/255 (28.24%), Postives = 116/255 (45.49%), Query Frame = 2 
Query:  464 VVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLL-LNYWP--LGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHV---------------ALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIF-----DHRKPTNN 1159
            +V L T+ GNLLV  S F  R LR+PTNY + SLAV+DLL+G  S+ F    LL    WP  LG   C  W+ +D      S   +  I+ DR  ++  P +YR   T  + LI++   WI  +S  F   + W   +     PQ     G +  V+  T++ +  F+  L+++   YG +  V               ALN+  + ++   + +   S+A   + R+    ++   +D  F      HR P ++
Sbjct:   32 IVMLLTIMGNLLVCYSVFYYRRLRSPTNYLVVSLAVSDLLVGTLSLPFRLAQLLNEENWPSHLGREGCQFWIWVDMLCSGASMVNLAGISFDRLLAIKQPLRYREKMTSKRALILILVVWIYAASCAFLSFVEWEGESTIAAYPQ----CGIRARVYI-TLMALAAFFCPLLIVIICYGMVLQVAIRHALQVQKEKEQIALNVSPEPNSNNHEESGETSLASPPVIRLKTSRSSSHRKDINFANGHAKHRNPASS 281
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Nematostella
Match: EDO36610 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI87])

HSP 1 Score: 85.5001 bits (210), Expect = 3.612e-18
Identity = 59/216 (27.31%), Postives = 106/216 (49.07%), Query Frame = 2 
Query:  467 VSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLN-YWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGW-----PFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQR-----KSDAKRRKMTSLVSMAGQT 1081
            + L T  GN +V+ +  +   +R  TNYF+ SLAV+DLL+   S+ F       N  W LG   C  W+ +D   C  S + +  I+VDRF ++ +P +Y    T    + ++ A WI+   +    ++ W     P F  +  C + +        V++  +  +G+F L L ++  +YG ++ VA+N  R     ++D+KRR   S +++  + 
Sbjct:    8 ILLVTFYGNFMVVLAVVLFHRMRTITNYFVVSLAVSDLLVATLSLPFRIHQTTHNTAWCLGLEACVAWIVVDIICCGASIWNLAMISVDRFIAIVMPFRYHALMTPMTGVCLIGAVWILSMGMGMLSLINWTELGSPHFHIEVQCRKFD-------PVYYTVVSVVGFF-LPLFIVLLMYGQVFVVAMNQARAVAALQTDSKRRGRRSSINLVKEV 215
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Nematostella
Match: EDO35450 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLG6])

HSP 1 Score: 82.8037 bits (203), Expect = 3.226e-17
Identity = 62/186 (33.33%), Postives = 95/186 (51.08%), Query Frame = 2 
Query:  482 VAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNY-WPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATW---IIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRK 1027
            V GNLLV  +    R LR  TNYFI SLAV+DLL+   SM F   + L +  W LG   C+ W+ +D      S   +  I+VDRF ++  P  Y +  T  + L+ +A  W   ++ S++ FT  L W       G  Q+      +   ++  ++T+G+F L L+V+   Y  ++ VAL+  RK
Sbjct:   24 VCGNLLVCGAILSYRRLRTVTNYFIISLAVSDLLVAGLSMPFRIHHTLHSMCWDLGISVCEFWIFVDLVCSSASICNLSMISVDRFLALSYPLTYPSIMTKRRGLLAIAMVWGYSLLISALSFT--LPW----SPDGLMQDIPACSLKDKYYYTFVITVGFF-LPLVVLVIAYSLVFKVALHQARK 202
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Nematostella
Match: EDO49899 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF54])

HSP 1 Score: 79.7221 bits (195), Expect = 2.257e-16
Identity = 66/209 (31.58%), Postives = 105/209 (50.24%), Query Frame = 2 
Query:  467 VSLATVAGNLLVITSFFVERALRNPT-NYFIASLAVTDLLIGIFSMNFYTIYLLLNY-WPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCI--LGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRK---SDAKRRKMTSLVSMA 1072
            V +  ++GN+LV T+  V   LR+ T NYFI SLAV+D++    +M F    +L  Y W  GE  C+++++    A   S   +  ++VDR+ ++  P +Y N  T   VL  + + W+   +I FT I  L WP +  ++      C   F    +++ I ++  F L  IVMC LY  IY VA    R+    D K  K  +++  A
Sbjct:    8 VIITALSGNILVCTAILVHERLRSSTTNYFIFSLAVSDIITASIAMPFDVDQILKKYTWEHGEAMCNIFVTSYLVAAPLSMLNLLAVSVDRYHAITQPLQYPNIMTPKVVLTSIFSLWV--YAILFTVIAMLAWPLY--EKSVFDGAC--HFNISPWYSLISSVVNFVLPTIVMCLLYFRIYKVAHAHARRIRGHDIKAAKTIAIIVSA 210

HSP 2 Score: 55.0694 bits (131), Expect = 4.304e-8
Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = 2 
Query: 2195 RTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVN---VHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDF 2443
            R      KA +TI +I+ AF++CW P+ ++       P   +      F ++  L Y NS +NPF YA  N  F+++F RL R  F
Sbjct:  192 RIRGHDIKAAKTIAIIVSAFLLCWMPYTLVSCASVICPPCFHRTPSELFTVTLLLAYINSALNPFLYAFHNTDFRESFRRLFRLQF 277
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Nematostella
Match: EDO36816 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHK5])

HSP 1 Score: 78.5666 bits (192), Expect = 5.536e-16
Identity = 64/213 (30.05%), Postives = 108/213 (50.70%), Query Frame = 2 
Query:  464 VVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLS---LDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKV-LIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALN------LQRKSDAKRRKMTSLVSMA 1072
            ++++  V GN LV+ SF     LR  TNYF+ASLAV D+++G+ S+  +T +++L+  PL   +    ++   LD  A +TS   +  I+++RF +V  P ++RN  T+  V  I +   W++P+            F G      +E +      V+  T+  + +F L L V+   Y GI+ V  N       QR S+ K R M  +V++A
Sbjct:   10 MIAILAVVGNALVVGSFIRHARLRTFTNYFVASLAVADIIVGLISIPMWTAFVILH--PLSLTYPGYVMTYTVLDIFAGITSILHLMVISLERFYAVAYPVRHRN--TNAWVYYICLVFVWVVPA-----------VFAG-----TSEHFRKADKKVYMITMFAV-FFLLPLFVILLAYAGIWRVVRNRVIPQATQRSSNLK-RDMRVIVTIA 200

HSP 2 Score: 54.6842 bits (130), Expect = 5.222e-8
Identity = 33/112 (29.46%), Postives = 53/112 (47.32%), Query Frame = 2 
Query: 2126 IYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGY----------YPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            I+R+  R R  P     +  +R     R  + + TI L++  FVI W PF I+ +L  +           P L + H       + Y NS +NP CYA+   +F++AF +L+
Sbjct:  170 IWRVV-RNRVIP-----QATQRSSNLKRDMRVIVTIALVIALFVISWLPFFIINMLITFCKNCVIGFKGLPPLYSAHLLLFVKVMHYFNSAVNPVCYAVKIPEFRRAFRQLV 275
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Medaka
Match: chrm3a (muscarinic acetylcholine receptor M3 [Source:NCBI gene;Acc:101164585])

HSP 1 Score: 156.377 bits (394), Expect = 1.850e-39
Identity = 94/311 (30.23%), Postives = 150/311 (48.23%), Query Frame = 2 
Query:  425 PLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKG-FH-WHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDN 1351
            P+    ++  L G +SL T+ GN+LV+ SF V R L+   NYF+ SLAV DL+IGI SMN YT YL++ YW +G   CD+WL++DY A   S   +  I+ DR+ S+  P  YR  RT  +  IM+   W +   ++   IL W +F G+R  P NEC+  F ++        +  F+L + +M  LY  IY    N  ++          L  + G                       +  ++  L  ++  +   ++  G FH WHG +  + GS       +  +   D+SSS ++ ++   VS+D+
Sbjct:   73 PVWQVVLIVLLTGTLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGIISMNLYTSYLIMGYWAMGNWACDIWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTRRAGIMIGLAWFVSFVLWAPAILLWQYFVGERTVPPNECYIQFFSEPITTFCTAMAAFYLPVTIMSVLYWRIYKETQNRSKE----------LAGLQGSVGRGGVGTRVGKLRNVHQTGSSRSCSSYELTRLSKKKSTCQELVGRFHCWHGVRSWRPGS------IRQGDGDPDQSSSDSWNNNDAAVSLDH 367

HSP 2 Score: 77.411 bits (189), Expect = 3.027e-14
Identity = 59/216 (27.31%), Postives = 100/216 (46.30%), Query Frame = 2 
Query: 1835 IKRKVDLQVSDDEKDTSVENNN---GHEQDPMGTENFVQRNSTKA---EGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQ----------IIYRITHRTRAKPCREPKEIFERGRTEN-RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            IK  V+ Q++  E    V   +   G EQ+P  + + V  N+T A   EG+ S+   +    +Q F+    ++L+K +  S+   R           +              I ++     +    R   +I +R R    + +KA +T++ IL AF+I WTP++I+VL+  +    +    + + YWLCY NS +NP CYAL N  F+  F  +L
Sbjct:  398 IKAAVNSQLTSCEDLDGVSEEDPLKGAEQNP-DSLSTVTSNTTAAKASEGINSTENSY----HQQFRSRQSQSLSKIQSNSNQGFRAGTPTIPTTASENTTTNTASKSPTVPISFKDAALAKRFAARTRTQITKRKRMSLLKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCEVCIPDTLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMIL 608
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Medaka
Match: chrm5a (muscarinic acetylcholine receptor M5 [Source:NCBI gene;Acc:101169892])

HSP 1 Score: 154.066 bits (388), Expect = 3.264e-39
Identity = 80/213 (37.56%), Postives = 118/213 (55.40%), Query Frame = 2 
Query:  362 LNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIY 1000
            LN T  A+T  +  V H L   + +  +    + +VS+ T+ GN+LV+ SF V   L+   NY++ SLA  DL+IG+FSMN YT Y+L+ YW LG + CDLWL+LDY A   S   +  I+ DR+ S+  P  YR  RT  +  IM+   W++   ++   IL W +F GKR  P+ +C   F ++        I  F++ + VM  LY  IY
Sbjct:    7 LNSTLNATTTDIHLVTHNLWEVITIATV----SAIVSIITIVGNILVMLSFKVNSQLKTVNNYYLLSLAAADLIIGVFSMNLYTSYILMGYWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPRRAAIMIGLAWLVSLILWAPPILCWQYFVGKRTVPERQCQIQFFSEPVITFGTAIAAFYIPVSVMTILYCRIY 215

HSP 2 Score: 85.5001 bits (210), Expect = 6.344e-17
Identity = 35/81 (43.21%), Postives = 54/81 (66.67%), Query Frame = 2 
Query: 2207 RARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            + RKA +T++ IL AF++ WTP++I+VL+  +  D + +  ++L YWLCY NS +NP CYAL N  F+K F  LL   + +
Sbjct:  419 KERKAAQTLSAILLAFILTWTPYNIMVLISTFCSDCIPLSLWHLGYWLCYVNSTVNPMCYALCNKTFQKTFRMLLLCQWKK 499
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Medaka
Match: ENSORLT00000022971.2 (muscarinic acetylcholine receptor M1-like [Source:NCBI gene;Acc:101171981])

HSP 1 Score: 150.984 bits (380), Expect = 1.119e-38
Identity = 94/304 (30.92%), Postives = 152/304 (50.00%), Query Frame = 2 
Query:  443 ILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRI--GVG---MTNDENEDEIFDH--RKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSD 1333
            I+ FL G +S+ TV GN+LV+ SF + + L+   NY++ SLA  DL+IG  SMN YT Y+++ +W LG + CDLWL++DY A   S   +  I+ DR+ SV  P  YR  RT  + + M+   W I   ++   IL W +  G+R  P  EC+  F T+        I  F+L + VM  L+  IY      +R  D +  K +   +   QT+S +  GVG   + +++    +  H  R  ++  +  +  P  D P+ S       +K ++S   ++ K  + +     +  SP F + S 
Sbjct:   16 IIVFLTGSLSVITVVGNILVLISFKINKTLKTVNNYYLLSLAFADLIIGTLSMNLYTTYIIMGHWALGPVLCDLWLAIDYVASNASVMNLLIISFDRYFSVTRPLTYRAKRTTKRTMAMIGLAWSISFVLWAPAILFWQYVVGERTVPPEECYIQFLTEPVTTFCTAIAAFYLPVTVMAFLFWKIYQE--TEKRAKDIQSLKGSCASTNLNQTVSEMSEGVGEGSIKSNQRNTSVKLHLVRTSSSRKLCHQDGPARD-PRGSD-----FDKFEKSVEEEENKDLEQTSTIKRQHGSPLFKASSS 311

HSP 2 Score: 69.3218 bits (168), Expect = 7.422e-12
Identity = 29/69 (42.03%), Postives = 44/69 (63.77%), Query Frame = 2 
Query: 2225 RTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            RT++ IL AF++ W P++I+VL+  +  + +    + L YWLCY NS +NP CYAL N  F+  F  +L
Sbjct:  353 RTLSAILLAFMMTWLPYNIMVLINTFCQNCIPEALWALGYWLCYVNSTVNPMCYALCNKTFRTTFRAIL 421
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Medaka
Match: chrm2a (cholinergic receptor muscarinic 2 [Source:NCBI gene;Acc:101157989])

HSP 1 Score: 150.214 bits (378), Expect = 2.273e-38
Identity = 76/196 (38.78%), Postives = 110/196 (56.12%), Query Frame = 2 
Query:  452 FLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAK 1039
             +AG +SL TV GN+LV+ S  V R L+   NYF+ SLA  DL+IG+ SMN YT+Y+++ YWPLG + CDLWL+LDY     S   +  I+ DR+  V  P  Y   RT     +M+AA W++   ++   IL W F  G R  P+ EC+  F ++        I  F+L +I+M  LY  I   + +  +K + K
Sbjct:   23 LVAGSLSLVTVIGNILVMLSIKVNRNLQTVNNYFLFSLACADLIIGLCSMNLYTVYIVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLSYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVPEKECYIQFFSNAAVTFGTAIAAFYLPVIIMIQLYWQISRASKSRVKKDNRK 218

HSP 2 Score: 82.4185 bits (202), Expect = 5.139e-16
Identity = 58/211 (27.49%), Postives = 92/211 (43.60%), Query Frame = 2 
Query: 1850 DLQVSDDEKDTSVENNNGHEQDPMG--------TENFVQRNSTKAEGVKSSHIQF-CEIPNQTF---QRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFH 2446
            + + S+D    SV  +N  +++P          T   + R   KA G K + I+   + P   +       ++ +  T+RQ+   R+I                          K  ++P    ++G   +R +K  RTI  IL AFV  WTP++++VL+  +    +    + + YWLCY NS INP CYAL N  FKK F  LL   + 
Sbjct:  274 EKESSNDSTSVSVVASNQKDEEPAPSTANSCAETSQQIPRQRAKAGGSKLTCIKIKTKSPKGDYYTPSNATVEIVPATERQNHVARKI-------------------------VKMTKQPPNKKKKG-APSREKKVTRTIMAILVAFVATWTPYNVMVLINTFCSSCIPNTVWTIGYWLCYINSTINPACYALCNVTFKKTFKHLLMCQYK 458
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Medaka
Match: ENSORLT00000036897.1 (muscarinic acetylcholine receptor M1-like [Source:NCBI gene;Acc:101171981])

HSP 1 Score: 150.214 bits (378), Expect = 2.834e-38
Identity = 80/224 (35.71%), Postives = 120/224 (53.57%), Query Frame = 2 
Query:  434 VTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGM 1105
            V  I+ FL G +S+ TV GN+LV+ SF + + L+   NY++ SLA  DL+IG  SMN YT Y+++ +W LG + CDLWL++DY A   S   +  I+ DR+ SV  P  YR  RT  + + M+   W I   ++   IL W +  G+R  P  EC+  F T+        I  F+L + VM  L+  IY      +R  D +  K +   +   QT+S +  G+
Sbjct:   41 VVIIIVFLTGSLSVITVVGNILVLISFKINKTLKTVNNYYLLSLAFADLIIGTLSMNLYTTYIIMGHWALGPVLCDLWLAIDYVASNASVMNLLIISFDRYFSVTRPLTYRAKRTTKRTMAMIGLAWSISFVLWAPAILFWQYVVGERTVPPEECYIQFLTEPVTTFCTAIAAFYLPVTVMAFLFWKIYQE--TEKRAKDIQSLKGSCASTNLNQTVSEMSEGV 262

HSP 2 Score: 69.3218 bits (168), Expect = 6.824e-12
Identity = 29/69 (42.03%), Postives = 44/69 (63.77%), Query Frame = 2 
Query: 2225 RTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLL 2431
            RT++ IL AF++ W P++I+VL+  +  + +    + L YWLCY NS +NP CYAL N  F+  F  +L
Sbjct:  370 RTLSAILLAFMMTWLPYNIMVLINTFCQNCIPEALWALGYWLCYVNSTVNPMCYALCNKTFRTTFRAIL 438
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Planmine SMEST
Match: SMESG000039095.1 (SMESG000039095.1)

HSP 1 Score: 1432.54 bits (3707), Expect = 0.000e+0
Identity = 703/704 (99.86%), Postives = 704/704 (100.00%), Query Frame = 2 
Query:  341 MSVSSGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWXXXXXXXXXXXXXXXDQGINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGFCPSTENNSPVWIKRKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            MSVSSGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPG+DNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWRHNNSYHHSNNRHSSDQGINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGFCPSTENNSPVWIKRKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT
Sbjct:    1 MSVSSGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGDDNPKKSKGFHWHGNKVDQSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLLTCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYRQMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWRHNNSYHHSNNRHSSDQGINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGFCPSTENNSPVWIKRKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQFCEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRITHRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 704
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Planmine SMEST
Match: SMESG000066813.1 (SMESG000066813.1)

HSP 1 Score: 327.02 bits (837), Expect = 4.218e-102
Identity = 151/224 (67.41%), Postives = 184/224 (82.14%), Query Frame = 2 
Query:  422 FPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGK-RGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSR 1090
            FP  V+  LGFL+ VVS++T+ GNLLVI SFF ERALR  TNYFIASLAVTDLLIG+FSMNFYT+YLLL+YWPLGE+FCDLWLSLDYTACLTSQYTVFFITVDRFCSV IPAKYRNWRT++KV +MVA TWI+P ++FFT I+GWP+F  + R    ++C+A F  +  FNTILTI YFW+TL+VM  LY GIY VALNLQ KSD K+++++ L+  + + M+ 
Sbjct:   18 FPPAVSWTLGFLSAVVSVSTIMGNLLVIVSFFFERALRTSTNYFIASLAVTDLLIGVFSMNFYTVYLLLSYWPLGELFCDLWLSLDYTACLTSQYTVFFITVDRFCSVKIPAKYRNWRTESKVRLMVAITWIVPFAVFFTTIMGWPYFNNQGRIREPDQCYAEFAKEPIFNTILTIAYFWITLMVMVILYAGIYKVALNLQAKSDHKKKRISELIPSSSKRMTE 241

HSP 2 Score: 135.961 bits (341), Expect = 2.181e-33
Identity = 63/102 (61.76%), Postives = 75/102 (73.53%), Query Frame = 2 
Query: 2165 REPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPD------LVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            +  K    R RT +RARKALRTIT ILGAFV+ WTPFHI+VL+KG+          VN H +NL+YWLCY NSPINPFCYALAN QFK+ FLR+L+ DF R+
Sbjct:  425 KSDKSTKRRERTVSRARKALRTITFILGAFVLSWTPFHIIVLIKGFCDSTKDKHTCVNSHLYNLTYWLCYLNSPINPFCYALANVQFKRTFLRILKFDFRRS 526
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Planmine SMEST
Match: SMESG000033502.1 (SMESG000033502.1)

HSP 1 Score: 318.161 bits (814), Expect = 3.735e-99
Identity = 162/284 (57.04%), Postives = 205/284 (72.18%), Query Frame = 2 
Query:  359 FLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMT--NDENEDEIFDHRKPTNNMVLDEMTPGEDNPKK 1204
            FLNGT  A     S  N +L   +P+  ++   + V SLAT+ GN+LV+ +F +E+++R P+NYFI SLA TDLLIG+ SMN +TIYL++  WPL ++ CDLWLSLDYTACLTSQYTV FITVDRFCSV IPA YR WRT NKV+IMV  +W++PSSIFFTCILGWP+F   R     EC+A F ++  FNT+LTI YFW+TLIVMC LY GIY VA  LQ  SD KR+ M +L++MAGQTMSRIGVG++  N ++ DEI +   PT N  ++E   G D+  K
Sbjct:    3 FLNGTLNAQ----SPRNDSL--SIPIIFLIVVSSSVASLATIGGNILVLIAFLLEKSIRTPSNYFIISLASTDLLIGLISMNLFTIYLIIGNWPLSQLLCDLWLSLDYTACLTSQYTVLFITVDRFCSVKIPATYRLWRTKNKVIIMVLISWLVPSSIFFTCILGWPYFNNGRQRGATECYAEFTSNTVFNTVLTIVYFWITLIVMCVLYAGIYRVAKRLQASSD-KRKGMNTLLAMAGQTMSRIGVGVSWGNADDLDEIAN---PTVNFRINE---GYDSSNK 273

HSP 2 Score: 144.436 bits (363), Expect = 1.992e-36
Identity = 63/85 (74.12%), Postives = 72/85 (84.71%), Query Frame = 2 
Query: 2195 RTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
            R+ NRARKALRTI+LILGAFV CWTP+H+LVL+KG+    VN H +NLSYWLCY NSPINPFCYALAN+Q K  F+RLLRGDF R
Sbjct:  402 RSRNRARKALRTISLILGAFVTCWTPYHVLVLIKGFCNSCVNDHLYNLSYWLCYINSPINPFCYALANSQIKTTFMRLLRGDFRR 486
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Planmine SMEST
Match: SMESG000043689.1 (SMESG000043689.1)

HSP 1 Score: 295.434 bits (755), Expect = 5.470e-89
Identity = 144/244 (59.02%), Postives = 184/244 (75.41%), Query Frame = 2 
Query:  455 LAGVVSLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMTNDENEDEIFDH-RKPTNNMVLDEMTP 1183
            L+  VS+ T+AGN+LVIT+F +E+ LR   NYFI SLA TDLLIG+FSMN +T+YLLL  WPLG+  CDLWLS+DYTACLTSQ TV  ITVDR+CSV IP  YR+WRT +KV+IMVA  W++PS +FFT ILGWP FT ++     EC+A F +   FNT+LT+GYFW+ L +M  LY GIY+VA NLQ+KSD + +KMT+L++MAGQTMSRIGVG+  + NE E  +H RK + + +  E  P
Sbjct:  169 LSVAVSVITIAGNILVITAFALEKKLRVIGNYFIVSLAATDLLIGLFSMNLFTVYLLLGCWPLGQFLCDLWLSVDYTACLTSQCTVLSITVDRYCSVKIPNIYRSWRTGSKVIIMVAIAWLVPSILFFTAILGWPLFTIRKRLIH-ECFADFTSQPVFNTLLTVGYFWVPLFIMLFLYYGIYNVACNLQKKSDERHKKMTTLMAMAGQTMSRIGVGVNWNNNESE--NHPRKDSGDYLQTEKHP 409

HSP 2 Score: 139.813 bits (351), Expect = 3.409e-34
Identity = 59/90 (65.56%), Postives = 74/90 (82.22%), Query Frame = 2 
Query: 2192 GRTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPD----LVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHR 2449
             R+ENRARKA RTI+LILGAF+ CWTP+H+LV++KG+  +     VN H +N+SYWLCY NSPINPFCYALAN QFK+ F+RL++GD HR
Sbjct:  537 SRSENRARKAFRTISLILGAFLACWTPYHVLVMIKGFCYNRSEICVNDHLYNISYWLCYMNSPINPFCYALANIQFKRTFIRLMKGDLHR 626
[Close]
BLAST of Muscarinic acetylcholine receptor vs. Planmine SMEST
Match: SMESG000027386.1 (SMESG000027386.1)

HSP 1 Score: 268.855 bits (686), Expect = 2.108e-80
Identity = 120/213 (56.34%), Postives = 160/213 (75.12%), Query Frame = 2 
Query:  470 SLATVAGNLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWPLGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKVLIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTILTIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSRIGVGMT 1108
            S+ TV GN L+IT+F +E+ +R P+NYFIASLA+TD+LIG+FSMN ++IY++L YWPLG + C++WLS DYT CLTSQYTV  ITVDR+CSV IPAKYR WRT+ KV +++   WIIP+ +F T I+ W   +        +C+A F     FNT+ TI YFW+TLI+MC LY GIY VA  L + SD KR+++TSL++ AGQTMS+IGVG++
Sbjct:   31 SIGTVFGNGLIITAFIMEKKIRIPSNYFIASLAITDILIGLFSMNLFSIYVVLGYWPLGYILCNIWLSFDYTVCLTSQYTVLLITVDRYCSVKIPAKYRIWRTEKKVYLLIVLAWIIPACLFITIIIIWNCRSQSPSVDSQQCFAEFTLYPIFNTVFTIAYFWITLIIMCVLYIGIYRVARRLHQNSDTKRKRLTSLMATAGQTMSKIGVGLS 243

HSP 2 Score: 123.635 bits (309), Expect = 2.574e-29
Identity = 51/90 (56.67%), Postives = 72/90 (80.00%), Query Frame = 2 
Query: 2195 RTENRARKALRTITLILGAFVICWTPFHILVLLKGYYPD----LVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGDFHRT 2452
            R  ++A+K+L+TI+ ILGAF+ICWTP+H+L+L+KG+  +     +N   +N SYWLCY NSPINPFCYAL+N+QFKK  L+L++GDF R+
Sbjct:  422 RNHSKAKKSLKTISFILGAFIICWTPYHVLILIKGFCDNPPKSCINDVLYNASYWLCYINSPINPFCYALSNSQFKKTLLKLIKGDFSRS 511
The following BLAST results are available for this feature:
BLAST of Muscarinic acetylcholine receptor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CHRM14.023e-4040.20cholinergic receptor muscarinic 1 [Source:HGNC Sym... [more]
CHRM14.402e-4040.20cholinergic receptor muscarinic 1 [Source:HGNC Sym... [more]
CHRM31.864e-3931.82cholinergic receptor muscarinic 3 [Source:HGNC Sym... [more]
CHRM31.864e-3931.82cholinergic receptor muscarinic 3 [Source:HGNC Sym... [more]
CHRM48.769e-3830.77cholinergic receptor muscarinic 4 [Source:HGNC Sym... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gar-21.121e-5345.07Muscarinic acetylcholine receptor gar-2 [Source:U... [more]
gar-21.446e-5244.86Muscarinic acetylcholine receptor gar-2 [Source:U... [more]
gar-21.811e-5244.86Muscarinic acetylcholine receptor gar-2 [Source:U... [more]
gar-22.360e-5244.86Muscarinic acetylcholine receptor gar-2 [Source:U... [more]
gar-14.926e-4337.96Probable muscarinic acetylcholine receptor gar-1 ... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mAChR-B7.185e-7844.09gene:FBgn0037546 transcript:FBtr0344726[more]
mAChR-B1.370e-7644.09gene:FBgn0037546 transcript:FBtr0081789[more]
mAChR-A9.489e-3926.72gene:FBgn0000037 transcript:FBtr0072367[more]
mAChR-A1.204e-3832.37gene:FBgn0000037 transcript:FBtr0072368[more]
5-HT1A6.393e-2431.51gene:FBgn0004168 transcript:FBtr0474126[more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrm1a3.278e-3937.56cholinergic receptor, muscarinic 1a [Source:ZFIN;A... [more]
chrm1a3.794e-3937.78cholinergic receptor, muscarinic 1a [Source:ZFIN;A... [more]
CR812847.14.504e-3937.78pep chromosome:GRCz11:CHR_ALT_CTG14_1_19:24256795:... [more]
chrm3a6.104e-3827.39cholinergic receptor, muscarinic 3a [Source:ZFIN;A... [more]
chrm2a6.413e-3834.98cholinergic receptor, muscarinic 2a [Source:ZFIN;A... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000048394.13.020e-3834.91pep primary_assembly:Xenopus_tropicalis_v9.1:5:599... [more]
CHRM21.008e-3730.52cholinergic receptor, muscarinic 2 [Source:NCBI ge... [more]
slitrk37.876e-3640.00SLIT and NTRK like family member 3 [Source:Xenbase... [more]
CHRM15.433e-3234.00cholinergic receptor, muscarinic 1 [Source:NCBI ge... [more]
cdkn2aip2.117e-2934.50CDKN2A interacting protein [Source:Xenbase;Acc:XB-... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Chrm12.466e-4040.20cholinergic receptor, muscarinic 1, CNS [Source:MG... [more]
Chrm12.466e-4040.20cholinergic receptor, muscarinic 1, CNS [Source:MG... [more]
Chrm33.899e-3931.49cholinergic receptor, muscarinic 3, cardiac [Sourc... [more]
Chrm33.899e-3931.49cholinergic receptor, muscarinic 3, cardiac [Sourc... [more]
Chrm41.261e-3734.82cholinergic receptor, muscarinic 4 [Source:MGI Sym... [more]
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BLAST of Muscarinic acetylcholine receptor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q09388|ACM2_CAEEL2.303e-5144.86Muscarinic acetylcholine receptor gar-2 OS=Caenorh... [more]
sp|Q18007|ACM1_CAEEL8.565e-4237.96Probable muscarinic acetylcholine receptor gar-1 O... [more]
sp|Q5R949|ACM1_PONAB9.241e-4038.65Muscarinic acetylcholine receptor M1 OS=Pongo abel... [more]
sp|P56489|ACM1_MACMU1.085e-3940.20Muscarinic acetylcholine receptor M1 OS=Macaca mul... [more]
sp|P12657|ACM1_MOUSE1.725e-3940.20Muscarinic acetylcholine receptor M1 OS=Mus muscul... [more]
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BLAST of Muscarinic acetylcholine receptor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KU580.000e+0100.00GCR010 OS=Schmidtea mediterranea OX=79327 GN=gcr01... [more]
A0A1S3HMP01.265e-13738.16muscarinic acetylcholine receptor M4 OS=Lingula un... [more]
K1QZ791.108e-12336.97Putative muscarinic acetylcholine receptor gar-2 O... [more]
A0A210QWZ44.446e-12038.68Muscarinic acetylcholine receptor gar-2 OS=Mizuhop... [more]
A0A193KUP48.529e-9951.70GCR071 OS=Schmidtea mediterranea OX=79327 GN=gcr07... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrm2a5.201e-3934.55cholinergic receptor muscarinic 2 [Source:NCBI gen... [more]
chrm2b2.784e-3838.99muscarinic acetylcholine receptor M2-like [Source:... [more]
chrm2b2.784e-3838.99muscarinic acetylcholine receptor M2-like [Source:... [more]
chrm5a1.621e-3739.46muscarinic acetylcholine receptor M5 [Source:NCBI ... [more]
chrm4a8.333e-3736.89cholinergic receptor muscarinic 4 [Source:NCBI gen... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CHRM35.016e-4231.00cholinergic receptor muscarinic 3 [Source:HGNC Sym... [more]
CHRM31.042e-4037.82cholinergic receptor muscarinic 3 [Source:HGNC Sym... [more]
chrm5b3.641e-3836.49cholinergic receptor, muscarinic 5b [Source:ZFIN;A... [more]
chrm4a5.253e-2940.86cholinergic receptor, muscarinic 4a [Source:ZFIN;A... [more]
HTR1F1.022e-2834.745-hydroxytryptamine receptor 1F [Source:HGNC Symbo... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO473591.730e-2228.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO366103.612e-1827.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO354503.226e-1733.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO498992.257e-1631.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO368165.536e-1630.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrm3a1.850e-3930.23muscarinic acetylcholine receptor M3 [Source:NCBI ... [more]
chrm5a3.264e-3937.56muscarinic acetylcholine receptor M5 [Source:NCBI ... [more]
ENSORLT00000022971.21.119e-3830.92muscarinic acetylcholine receptor M1-like [Source:... [more]
chrm2a2.273e-3838.78cholinergic receptor muscarinic 2 [Source:NCBI gen... [more]
ENSORLT00000036897.12.834e-3835.71muscarinic acetylcholine receptor M1-like [Source:... [more]
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BLAST of Muscarinic acetylcholine receptor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000039095.10.000e+099.86SMESG000039095.1[more]
SMESG000066813.14.218e-10267.41SMESG000066813.1[more]
SMESG000033502.13.735e-9957.04SMESG000033502.1[more]
SMESG000043689.15.470e-8959.02SMESG000043689.1[more]
SMESG000027386.12.108e-8056.34SMESG000027386.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020987 ID=SMED30020987|Name=Muscarinic acetylcholine receptor|organism=Schmidtea mediterranea sexual|type=transcript|length=2566bp
CTTCCGATCTCTTACGACAAGTAGAACCAGAGTTGCTATCATCCTTGGAC
GTATCCACATCTGTCAAGGTCTTTAATGGAGATATTTCGGCTTTTCCTCG
TTATCGCTAATATAAAATACTTATTTTTTATGTTTAAATGAAATGATTAT
AATTAAATTTTTTATTTTTCAAAAAACACAGATTTTGGCCTCTAAAAACA
CTAAACGTCGGCAGTAGGCCACTATTGTAAGATCGTTTGCTGCATTTTTG
GCTTGCATAATGACCAAAAATATGGATATGAATGAAATTTTTTGACAATA
AAATTATGTAGAAGCAAATTTCTTTCTTCAACTTTTTACAATGTCAGTTT
CAAGTGGATTTCTTAATGGTACAACAACGGCATCAACTATTATTCTCAGT
AAAGTCAATCATACATTGCTTTTCCCATTGCCTGTAACAGCAATCCTCGG
ATTTCTAGCTGGAGTTGTCAGTTTGGCAACCGTCGCTGGTAACTTGCTAG
TAATTACATCGTTTTTCGTTGAAAGGGCTTTGAGAAATCCGACGAATTAT
TTTATTGCATCATTAGCCGTGACGGACCTTTTGATTGGAATATTTTCGAT
GAACTTCTATACGATTTATTTGCTTTTGAACTATTGGCCATTGGGTGAAA
TGTTTTGTGATTTATGGTTGTCGTTGGATTATACAGCTTGTTTAACGTCT
CAATATACCGTATTTTTTATAACTGTCGATCGTTTTTGTTCAGTCCACAT
TCCTGCAAAATACAGAAATTGGAGAACTGACAACAAAGTCCTAATCATGG
TAGCGGCAACTTGGATAATTCCTTCTTCGATATTTTTCACTTGCATTCTT
GGTTGGCCGTTTTTTACCGGCAAACGAGGCTGCCCACAAAACGAATGTTG
GGCTGGATTTCAAACCGATGTTTTTTTCAACACAATTCTAACAATAGGTT
ATTTCTGGTTGACTTTAATCGTCATGTGTAGCCTTTATGGGGGAATTTAT
CATGTTGCATTGAATCTCCAAAGAAAGTCTGATGCTAAAAGAAGAAAAAT
GACTAGTCTTGTTTCAATGGCTGGACAAACAATGTCCAGAATCGGAGTTG
GAATGACAAACGATGAGAACGAAGATGAAATATTTGACCATCGTAAACCA
ACAAATAATATGGTTCTTGATGAAATGACACCTGGTGAAGACAATCCTAA
GAAGTCGAAAGGATTTCATTGGCATGGCAACAAGGTTGATCAATCAGGAA
GTCATCAGAAATTAAAACAAAAGAAACATTCAGAAAAACAAGATGATCGA
TCTAGTAGCCCTACCTTTCCTTCTGATTCTGATATCGTATCAATTGATAA
TCTTCAACAAGGTCCTAAAACAACGGATACTGTTTTATTAACTTGTCAAG
ACTTAATTATAACAAAGAAGATTCAATCTCCTGTTATTTTAGATGATTCT
CCAAGTCCATCAGAAACTTCTCCGTTTAATTTCTTCCAATATGGTAACAT
GAGTACGGTTACAACCACATCAAGCTCAAGATTTTATCGTCAAATGAAGA
ACAACTACATAGGAGCTGTTTCAGCTTATTCAAATGACTCCGATTATCAA
ACCGCCAGTGGATTTTCTACCGAATACGTTTCAGAATGGCGTCATAATAA
CTCATATCATCACAGTAATAATAGGCACAGTTCTGATCAAGGAATTAATA
AATCTAATACAACAGCTACTGGTCTACAATATATAGATTCAAATAGTCTT
CAGAATTCAAGATCCAAATTGATATTGAACATTTCTCCTGCAAACGGATT
TTGTCCATCTACCGAAAATAATTCTCCTGTGTGGATCAAAAGGAAAGTTG
ACTTACAGGTTTCAGATGATGAAAAAGACACGTCCGTTGAGAACAACAAC
GGTCATGAGCAAGATCCCATGGGAACCGAAAATTTTGTTCAACGTAATTC
TACTAAAGCTGAAGGCGTCAAATCGAGTCACATTCAATTCTGTGAAATTC
CTAATCAAACTTTTCAAAGGCACGATATCAAAACATTGAACAAAACTAAA
CGACAGTCATCGTATCGAAGACGCATCAATTTAAGAGCATTCTTCCTTCG
AGTTCGAGATATCATTCAACAGATCATTTATCGAATCACTCATCGAACGA
GAGCAAAACCTTGCCGAGAACCAAAAGAAATTTTCGAGCGTGGTCGAACT
GAAAACCGAGCGAGAAAAGCATTGAGGACAATAACATTAATTCTCGGAGC
TTTTGTCATTTGTTGGACTCCTTTTCACATATTGGTATTGCTGAAAGGAT
ATTATCCAGATCTGGTCAATGTTCATTTTTTCAATCTTTCATATTGGCTA
TGCTATGCTAACAGTCCGATTAATCCTTTTTGTTATGCTTTGGCCAATGC
ACAATTCAAGAAAGCATTCTTAAGATTACTTCGGGGTGACTTTCATCGGA
CATGATGAAAAAAGATTTTTTTACGGATAAAAAAGGATATTTCGAATTCA
AATCATTAGTTTTAGTAAAAACAGAAGATTGTTTTAAACGAAATAGCATA
AAAATTAGATTCTTTT
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protein sequence of SMED30020987-orf-1

>SMED30020987-orf-1 ID=SMED30020987-orf-1|Name=SMED30020987-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=705bp
MSVSSGFLNGTTTASTIILSKVNHTLLFPLPVTAILGFLAGVVSLATVAG
NLLVITSFFVERALRNPTNYFIASLAVTDLLIGIFSMNFYTIYLLLNYWP
LGEMFCDLWLSLDYTACLTSQYTVFFITVDRFCSVHIPAKYRNWRTDNKV
LIMVAATWIIPSSIFFTCILGWPFFTGKRGCPQNECWAGFQTDVFFNTIL
TIGYFWLTLIVMCSLYGGIYHVALNLQRKSDAKRRKMTSLVSMAGQTMSR
IGVGMTNDENEDEIFDHRKPTNNMVLDEMTPGEDNPKKSKGFHWHGNKVD
QSGSHQKLKQKKHSEKQDDRSSSPTFPSDSDIVSIDNLQQGPKTTDTVLL
TCQDLIITKKIQSPVILDDSPSPSETSPFNFFQYGNMSTVTTTSSSRFYR
QMKNNYIGAVSAYSNDSDYQTASGFSTEYVSEWRHNNSYHHSNNRHSSDQ
GINKSNTTATGLQYIDSNSLQNSRSKLILNISPANGFCPSTENNSPVWIK
RKVDLQVSDDEKDTSVENNNGHEQDPMGTENFVQRNSTKAEGVKSSHIQF
CEIPNQTFQRHDIKTLNKTKRQSSYRRRINLRAFFLRVRDIIQQIIYRIT
HRTRAKPCREPKEIFERGRTENRARKALRTITLILGAFVICWTPFHILVL
LKGYYPDLVNVHFFNLSYWLCYANSPINPFCYALANAQFKKAFLRLLRGD
FHRT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR000995Musac_Ach_rcpt
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0005887integral component of plasma membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
GO:0007165signal transduction
GO:0007213G-protein coupled acetylcholine receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
GO:0016907G-protein coupled acetylcholine receptor activity
GO:0004871signal transducer activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000995Muscarinic acetylcholine receptor familyPRINTSPR00243MUSCARINICRcoord: 35..47
score: 38.8
coord: 170..180
score: 47.04
coord: 685..699
score: 42.03
coord: 83..93
score: 67.98
coord: 654..672
score: 35.01
coord: 133..147
score: 38.84
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 197..220
score: 23.05
coord: 35..59
score: 26.91
coord: 68..89
score: 36.44
coord: 113..135
score: 22.82
coord: 627..651
score: 33.69
coord: 664..690
score: 31.66
IPR000276G protein-coupled receptor, rhodopsin-likeSMARTSM013817TM_GPCR_Srsx_2coord: 44..406
e-value: 5.7E-5
score: -36.2
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 50..682
e-value: 4.5E-65
score: 219.6
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 119..135
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 620..696
e-value: 8.2E-26
score: 93.0
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 38..225
e-value: 6.7E-60
score: 205.2
NoneNo IPR availableGENE3DG3DSA:1.10.530.40coord: 226..259
e-value: 6.7E-60
score: 205.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 323..338
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 298..338
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 298..322
NoneNo IPR availablePANTHERPTHR24248:SF137coord: 619..700
coord: 3..247
NoneNo IPR availablePANTHERPTHR24248ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTORcoord: 619..700
coord: 3..247
NoneNo IPR availableCDDcd153027tmA_mAChR_GAR-2-likecoord: 35..230
e-value: 8.08373E-97
score: 297.81
NoneNo IPR availableCDDcd153027tmA_mAChR_GAR-2-likecoord: 619..693
e-value: 9.15028E-36
score: 134.1
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 22..242
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 614..700
NoneNo IPR availableTMHMMTMhelixcoord: 202..224
NoneNo IPR availableTMHMMTMhelixcoord: 627..649
NoneNo IPR availableTMHMMTMhelixcoord: 150..172
NoneNo IPR availableTMHMMTMhelixcoord: 664..686
NoneNo IPR availableTMHMMTMhelixcoord: 107..129
NoneNo IPR availableTMHMMTMhelixcoord: 35..57
NoneNo IPR availableTMHMMTMhelixcoord: 70..92
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 50..682
score: 30.943