DNA topoisomerase 2

Overview
NameDNA topoisomerase 2
Smed IDSMED30020974
Length (bp)4540
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA topoisomerase 2 (SMED30020974) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA topoisomerase 2 (SMED30020974) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA topoisomerase 2 (SMED30020974) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA topoisomerase 2 (SMED30020974) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30020974

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SmedASXL_017863SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SmedASXL_017863SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30020974SMESG000037559.1 SMESG000037557.1 SmedASXL_033464SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 Contig6222newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 Contig6222uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020974SMESG000077843.1 SMESG000075930.1 SMESG000072474.1 SMESG000071411.1 SMESG000070882.1 SMESG000049741.1 SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SMESG000034944.1 SMESG000021315.1 SMESG000020394.1 SMESG000011999.1 Contig18945uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020974SMESG000077843.1 SMESG000075930.1 SMESG000072474.1 SMESG000071411.1 SMESG000070882.1 SMESG000049741.1 SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SMESG000034944.1 SMESG000021315.1 SMESG000020394.1 SMESG000011999.1 Contig18945newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30020974SMESG000037559.1 SMESG000037557.1 dd_Smed_v4_4804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30020974SMESG000037559.1 SMESG000037557.1 dd_Smed_v4_4804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30020974SMESG000037559.1 SMESG000037557.1 dd_Smed_v6_4804_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
neoblastSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SMESG000070153.1 SMESG000068232.1 SMESG000077027.1 dd_Smed_v6_1626_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SMESG000070153.1 SMESG000068232.1 SMESG000077027.1 dd_Smed_v6_1626_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30020974SMESG000037559.1 SMESG000037557.1 dd_Smed_v4_4804_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30020974SMESG000037559.1 SMESG000037557.1 SMED_13275_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30020974SMESG000037559.1 SMESG000037557.1 SMED_13275_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SMED_13275_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30020974SMESG000037565.1 SMESG000037559.1 SMESG000037557.1 SMED_13275_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.000e+0
Identity = 706/1203 (58.69%), Postives = 898/1203 (74.65%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP-GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   M  +++T+ P LYKIFDEILVNA+DNK RD  MT I++ ID + N++ IWNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    + +I     ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A   +G+ V  NGK++ V  F+ YVDLY K  LD     LKV++E   +RW+V +T S  + F Q+SFVNSIATTKGG HV+Y+ DQ+  K++E  KKK K         P QVK ++W+F+ CLIENP+FDSQTKENMTL  K FGS+C L+EKF    + CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE  EW+K       W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E +LYG  TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G  P  M P Y+NF GTI E+ +N+Y V+GEI  +D  T+EITELP+R WT  YKE V+E   +G DKTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M+LFD  G ++KY    +IL+ FF +RL  Y  RK +L+G+L A+++KL NQARFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK  A ++ E +NQ D   S+      PDF Y+  M + +LT EK + L+K+RD K +E++ ++ KSP  +W +DL    +E++ V +Q+  +    +    +K    K         ET PS + RRI P+I
Sbjct:   51 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEI 1246          
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990])

HSP 1 Score: 1391.71 bits (3601), Expect = 0.000e+0
Identity = 706/1203 (58.69%), Postives = 898/1203 (74.65%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP-GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   M  +++T+ P LYKIFDEILVNA+DNK RD  MT I++ ID + N++ IWNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    + +I     ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A   +G+ V  NGK++ V  F+ YVDLY K  LD     LKV++E   +RW+V +T S  + F Q+SFVNSIATTKGG HV+Y+ DQ+  K++E  KKK K         P QVK ++W+F+ CLIENP+FDSQTKENMTL  K FGS+C L+EKF    + CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE  EW+K       W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E +LYG  TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G  P  M P Y+NF GTI E+ +N+Y V+GEI  +D  T+EITELP+R WT  YKE V+E   +G DKTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M+LFD  G ++KY    +IL+ FF +RL  Y  RK +L+G+L A+++KL NQARFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK  A ++ E +NQ D   S+      PDF Y+  M + +LT EK + L+K+RD K +E++ ++ KSP  +W +DL    +E++ V +Q+  +    +    +K    K         ET PS + RRI P+I
Sbjct:   46 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEI 1241          
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2A (DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989])

HSP 1 Score: 1378.23 bits (3566), Expect = 0.000e+0
Identity = 689/1158 (59.50%), Postives = 881/1158 (76.08%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKL-------LAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3548
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   +  +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV  HK  ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+S+EY K FKQTW +NM    + E+     ED+TC+TF PDL +F M  LD+DIVAL  RRAYDIA  T+ + VFLNG ++ VK F+ YVD+Y K  LD     LKV++E    RWEV +T S  + F Q+SFVNSIAT+KGG HV+Y+ADQ+  K+++  KKK K           QVK +MWIFV  LIENP+FDSQTKENMTL  K FGS C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K  ++K +PKLDDAN AG +NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + YE  +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LL+H F+E+FITPIVK  KN Q ++FYS+PE  EW+  TP   +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED  +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F   D+  LK L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ +++++N++R+++G  P+ M P Y+NF GTI E+  N+YV++GE+  L+ TT+EI+ELP+R WT +YKE V+E   +G +KTP  ITDY+EY+TD TV+F+V+M   K AEAE VG HK FKLQT LT ++M+LFD  G ++KY+  ++IL  FF++RL+ Y  RK +LLG+L A+++KL NQARFILEKI+GK+I EN     LI  L+   YDSDPVKAW+++ +K+        + ++ E E  + + DS  P F YL  M +  LT EKKD L + R+EK +ELD ++ KSP  +W +DL    +E+  V
Sbjct:   30 VERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMS-EKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSG-PTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAV 1181          
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990])

HSP 1 Score: 1366.67 bits (3536), Expect = 0.000e+0
Identity = 678/1112 (60.97%), Postives = 850/1112 (76.44%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLT 3416
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   M  +++T+ P LYKIFDEILVNA+DNK RD  MT I++ ID + N++ IWNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    + +I     ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A   +G+ V  NGK++ V  F+ YVDLY K  LD     LKV++E   +RW+V +T S  + F Q+SFVNSIATTKGG HV+Y+ DQ+  K++E  KKK K         P QVK ++W+F+ CLIENP+FDSQTKENMTL  K FGS+C L+EKF    + CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE  EW+K       W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E +LYG  TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G  P  M P Y+NF GTI E+ +N+Y V+GEI  +D  T+EITELP+R WT  YKE V+E   +G DKTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M+LFD  G ++KY    +IL+ FF +RL  Y  RK +L+G+L A+++KL NQARFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK  A ++ E +NQ D   S+      PDF Y+  M + +LT
Sbjct:   46 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLT 1150          
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2A (DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989])

HSP 1 Score: 170.244 bits (430), Expect = 3.758e-48
Identity = 76/131 (58.02%), Postives = 108/131 (82.44%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVT 488
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   +  +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV  HK  ++Y+P +IFGQLLTS+N++DD+K+VT
Sbjct:   18 VERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVT 147          
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: top-2 (DNA topoisomerase 2 top-2 [Source:UniProtKB/Swiss-Prot;Acc:Q23670])

HSP 1 Score: 1305.04 bits (3376), Expect = 0.000e+0
Identity = 669/1224 (54.66%), Postives = 883/1224 (72.14%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKL-MWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAE-AVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAI-DKGEEENQED------------IEDSNKP------DFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDL-----EILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIE 3689
            IE +YQKK+QLEHILLRPD+Y+GS++  +K  MWV++ E++++ ++ I+Y P LYKI+DEILVNA+DNK RD KM TI+I I+ + N + ++NNG+GIPVT HK  +VY+P +IFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS++F LETSS++Y  +FKQTW  NM    +P+I K+  EDFT +TF PDL +F M  LD+DI  L +RRAYD+A  ++G+ VFLNGK+I +K F+DYV +YT    +   EPLK+ YE  G RW+VA+  S  + F QVSFVNSIATTKGG HV+Y+ADQ+ AK ++  K+K+      +K  P Q+K +MW+FV CLIENP+FDSQTKE MTL QK FGS C L+EKF    +  GI + +  W  FK    L+KKCSKTK +KLK +PKL+DAN AGTKNS QCTLILTEGDSAK+LAVSGL+V+GRD YGVFPLRGKLLNVRE  +KQ+ +NAE+  +IKI+GLQY +KYE  +  KTLRYGKLM+M DQDQDGSHIKGL+INFIH  WP+L++ NFVE+FITPIVKA K  + +SFYS+PE  EW+  T     +++KYYKGLGTSTSKEAKEYF DM RH I+F+Y+G +D  ++ +AFSK K +ERK+WL+ WM + K R++ G+ E YLY K T+++TF++F+N+EL+LFSN DNERSIPCL+DGFKPGQRKVLFAC KR   +E+KVAQLAG+VAE+SAYHHGE SLM TI+NLAQD+VGSNNINLL+P GQFGTRL GGKDSAS RYI TQLS +T+ +F   D+ VL+ L ++NQ IEPEWY PIIPMVL+NG  GIGTGWS+ IPN+NP++++ N+KR+I G     + PWY+NF G I+++  +++   GE+  LD  T+EITELPI+ WT  YKE V+E     +DK    I DYKEY+TD TV+F+V+++P K  E E     H+ FKLQ  +  + M+LFD  G +R Y +   I + F+  R E Y++RK YLLG+L AQ+ +L NQARFIL KIN +++ EN K A ++D L+  K+D+DPVK W K  +KL  + + GE E  ED            I  + K       D+ YL  M ++ L+ E+K+ L+KE +EK+ E+ +I  K+ Q +W +DL     E+ ++E     +QD S        +        G    +  E  PSK  +RIEP ++
Sbjct:   63 IEDIYQKKSQLEHILLRPDTYIGSVEHTEKTPMWVYNMEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQ-FNNEGEPLKIAYEQVGDRWQVALALS-EKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLTKTSMNIK--PFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILAKINNEIVLENKKKAAIVDVLIKMKFDADPVKKW-KEEQKLKELRESGEIELDEDDLAAVAVEEDEAISSAAKAVETKLSDYDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKKTWQDLWHEDLDNFVSELDKQEAREKADQDAS-IKNAAKKLAADAKTGRGPKKNVCTEVLPSKDGQRIEPMLD 1280          
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534])

HSP 1 Score: 823.543 bits (2126), Expect = 0.000e+0
Identity = 453/1133 (39.98%), Postives = 690/1133 (60.90%), Query Frame = 3
Query:  108 YQKKTQLEHILLRPDSYVGSIK-KEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKE---DFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAAC-TQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQ--------VSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGS--ECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKR-NLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMING-GTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQL-------DGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMN---PAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTA 3419
            Y+KK+  EH+LLRPD+Y+G +  +ED+++W+ D E  +M+ K++TY P L KIFDEILVNA+DNK RDS M  + + +D +   + +WNNG G+PV IH    +Y+PT++FG L TS+N++D + +  GGRNGYGAKLCNIFS EF++ET      + F+Q W +NM  C + E+ +   E   D+T V F PDL+RF + +L +D++ L  RR +++AA   + + V+LNG++ +V  F+DYV ++         +   +++     RW V V    N  F +        VSFVN+I T KGG+HV+Y+ D      + K     K       +KPA +K N+ IF+ CLIENPSF+SQTKE +T   K+FGS  EC   +K      + G++E +    L  N         +   + ++D+ KL+DA +AG T  + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV      + ++N EI+N+++I+GL++     +  + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH  WP+L+  + F++ F TP++KA K ++V SF+S+ E  +W  V  G  +W++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G  D  +I +AF + K+DERKEW+        RR E   +E+     G   I+++ F++ +L+ F   D +RSIP L+DG KP QRK+L+  L   +   E+KV+QLAG+VA   +YHHGE SL+ TII + Q F GS+N+ LL P GQFGTR  GG D+AS RYI T L+  T+L+F   D+ +L+  +++   +EP W  PI+P++LING +GIGTGWS+KI N NP D+ID ++R I+   T  ++ P+Y  F G +  V   K++ +G+I  +          ++EI ELPI +WT+ YKE + +I     +  P  + ++ E +T+K V F + ++    +++ +        +FKL+T LT  N +LFD  G ++++ N  EI   FF+VR ++Y KR         A+   ++NQ  FI    NG +   ++    L +KL    +  DP+    K                    +S K ++GYL +M +  LT+
Sbjct:   47 YEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFN--------DSSSLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD--KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDA-KKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWADVEEGG-KWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRSIPSLIDGLKPSQRKILWTLLNNMDESTEIKVSQLAGAVAHRQSYHHGEESLVRTIIRMGQTFCGSSNLPLLQPIGQFGTRHEGGNDAASARYIFTALAPTTRLLFPQADDDLLQKNVEEGMVVEPTWLCPIVPLILINGTEGIGTGWSTKIANRNPIDIIDMIRRKIDSISTEYEIPPFYEEFRGKLEVVTPTKFISSGKIQLIRPERKNASTFSIEIVELPIGIWTSKYKEKLSKIV----ETLP--VLEFSERHTEKRVHFRITLDRKKSSRFLQKSNSDLLNYFKLRTSLT-ENRVLFDRNGELKEFGNISEIAAEFFEVRRDLYEKRLKIQKEECEAKLIYVENQLNFIEMVTNGTIEIRSMGRNQLEEKLQEMGFRVDPMATIAK--------------------NSKKANYGYLLEMPVSRLTS 1124          
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: cin-4 (DNA topoisomerase-like protein cin-4 [Source:UniProtKB/Swiss-Prot;Acc:G5ECQ8])

HSP 1 Score: 766.533 bits (1978), Expect = 0.000e+0
Identity = 385/755 (50.99%), Postives = 524/755 (69.40%), Query Frame = 3
Query: 1335 MPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAE-AVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEE-------------ENQEDIEDSNKP------DFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEI 3539
            +PKL+DAN AGTKNS QCTLILTEGDSAK+LAVSGL+V+GRD YGVFP R KLLNV +  V Q+ ++AE+  IIKI+GLQY++KYE  +  KTLRYGKL+IM + D DGS  KGLL+NF H  WP L K +FVE FITPI KA +  + +SFYS+PE  EW+  T     + +KYY GLGT TSKEAK+ FSDM RH I+F+Y+G +D K++ +AFSK K + R ++L   M+D  +R++ G+ E  LY K T+++T ++F N E++    + N  SIPCL+DG KPGQRKVLFAC KR   +E+KVAQLAG+VAE+SAYHHGE SLM TI+NLAQD+VGS+NINLL+P GQFGTRL GGKDSAS R I  QLS++T+ +F   D+ VL+ L ++NQ IEPEWY PIIPMVL+NG  G GTGWS+ IPN+NP++++ N+KR+I G     + PWY+NF G I+++  +++   GE++ LD  T+EITELPI+ WT  YKE V+E     +DK    I DYKEY+TD TV+F+V+++P K  E E     H+ FKLQ  +  + M+LFD  G +R Y +   I + F+  R E Y++RK YLLG+L AQ+ +L NQARFIL  IN K++ EN K   ++D L+  K+D+DPVK W++  +KL  + +  E             E  EDI  + K       D+ YL  + ++ L+ E+K+ L+KE +EK+ E+ ++  K+ Q +W+ DL+    E+
Sbjct:   40 IPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPFRRKLLNVCDLNVNQIADSAEVNAIIKILGLQYTKKYETEDDFKTLRYGKLLIMANHDSDGSQFKGLLLNFFHRFWPALFKRDFVEDFITPIAKATEGKEEVSFYSLPEYSEWRMNTDNWKSYTIKYYNGLGTLTSKEAKKCFSDMVRHRIRFKYNGADDDKAVNMAFSKKKIEARTDYLMKLMQDKNQRKQQGLAEECLYNKETRFVTLKDFFNYEIVC---SWNLHSIPCLVDGLKPGQRKVLFACFKRANKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSHNINLLLPIGQFGTRLQGGKDSASARSIFAQLSQVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGTGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVSVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGWLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILATINNKIVLENKKKTAIVDVLIKMKFDADPVKKWKED-QKLKELRESGEIELDEDDLAAVAVEEGEDISSAAKAVETKLSDYDYLVGLALIKLSEEEKNKLIKESEEKMAEVRVLEKKTWQDLWITDLDNFMSEL 790          
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534])

HSP 1 Score: 556.984 bits (1434), Expect = 1.758e-180
Identity = 296/703 (42.11%), Postives = 449/703 (63.87%), Query Frame = 3
Query:  108 YQKKTQLEHILLRPDSYVGSIK-KEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKE---DFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAAC-TQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQ--------VSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGS--ECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERS 2165
            Y+KK+  EH+LLRPD+Y+G +  +ED+++W+ D E  +M+ K++TY P L KIFDEILVNA+DNK RDS M  + + +D +   + +WNNG G+PV IH    +Y+PT++FG L TS+N++D + +  GGRNGYGAKLCNIFS EF++ET      + F+Q W +NM  C + E+ +   E   D+T V F PDL+RF + +L +D++ L  RR +++AA   + + V+LNG++ +V  F+DYV ++         +   +++     RW V V    N  F +        VSFVN+I T KGG+HV+Y+ D      + K     K       +KPA +K N+ IF+ CLIENPSF+SQTKE +T   K+FGS  EC   +K      + G++E +    L  N         +   + ++D+ KL+DA +AG T  + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV      + ++N EI+N+++I+GL++     +  + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH  WP+L+  + F++ F TP++KA K ++V SF+S+ E  +W  V  G  +W++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G  D  +I +AF + K+DERKEW+        RR E   +E+     G   I+++ F++ +L+ F   D +R+
Sbjct:   47 YEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFN--------DSSSLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD--KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDA-KKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWADVEEGG-KWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721          
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534])

HSP 1 Score: 556.984 bits (1434), Expect = 1.758e-180
Identity = 296/703 (42.11%), Postives = 449/703 (63.87%), Query Frame = 3
Query:  108 YQKKTQLEHILLRPDSYVGSIK-KEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKE---DFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAAC-TQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQ--------VSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGS--ECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERS 2165
            Y+KK+  EH+LLRPD+Y+G +  +ED+++W+ D E  +M+ K++TY P L KIFDEILVNA+DNK RDS M  + + +D +   + +WNNG G+PV IH    +Y+PT++FG L TS+N++D + +  GGRNGYGAKLCNIFS EF++ET      + F+Q W +NM  C + E+ +   E   D+T V F PDL+RF + +L +D++ L  RR +++AA   + + V+LNG++ +V  F+DYV ++         +   +++     RW V V    N  F +        VSFVN+I T KGG+HV+Y+ D      + K     K       +KPA +K N+ IF+ CLIENPSF+SQTKE +T   K+FGS  EC   +K      + G++E +    L  N         +   + ++D+ KL+DA +AG T  + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV      + ++N EI+N+++I+GL++     +  + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH  WP+L+  + F++ F TP++KA K ++V SF+S+ E  +W  V  G  +W++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G  D  +I +AF + K+DERKEW+        RR E   +E+     G   I+++ F++ +L+ F   D +R+
Sbjct:   47 YEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFN--------DSSSLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD--KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDA-KKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWADVEEGG-KWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721          
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0332320)

HSP 1 Score: 1342.02 bits (3472), Expect = 0.000e+0
Identity = 677/1173 (57.72%), Postives = 870/1173 (74.17%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYA--EAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAI-----------------DKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEIN 3542
            IE++YQKK+QLEHILLRPDSY+GS++   +LMWV+D+ +N MV+K+I++ P LYKIFDEILVNA+DNK RD  M TI+IDID + NMV +WNNG+GIPVT+HKE ++Y+PTMIFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS+ F +ET+++EY KSFKQTW NNM    D +I      D+T +TF PDL +F M RLDEDIVAL SRRAYD+AA ++G++VFLNG ++ V+ FKDY+DL+ K+  D +  P+K+V+E   +RWEVA   S +  F QVSFVNSIAT KGG HV+++ D L  ++LE  KKK K         P QV+ ++W+FV CLIENP+FDSQTKENMTL QK FGS+CTL+EKFI+ ++K GIVE +  W  FK Q     K    K +K+K +PKL+DAN AG KNS++CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGKLLNVREA  KQL+ENAEI N+ KIIGLQY +KY   + LKTLRYGK+MIMTDQDQDGSHIKGLLINFIH+ WP LL+  F+E+FITPIVKA K N+ LSFYS+PE  EW+  T   + + +KYYKGLGTSTSKEAKEYF DM+RH I F+Y G  D +SIV+AFSK   + RK WLTN M++ KRR+ELG+ E YLY KGTK IT+ +FIN EL+LFSN DNERSIP L+DG KPGQRKV+F C KRN  +E+KVAQL+GSVAE+SAYHHGE SL  TI+NLAQ+FVG+NNINLL P GQFGTRL+GGKD AS RYI T +S LT+LI+H  D+ +L   +DD Q IEP WY PIIPMVL+NG +GIGTGWS+KI N+NP++++ NL++MING  P  MHPWY+NF G +  V + +Y+  G I  L G  LEI+ELP+ VWT +YKE+V+E   +G +K    I++Y+EY+TD TVRF++   P ++    AE  GF++ FKL T L+ + M  FD+   +R++   I+IL+ ++K+R E Y +R+++L+G L+AQA +L +QARFILEK   K++ EN +   + D+L+   Y  DPVK WQ+ ++   A                   K ++E + D E + K       F YL  M +  LT EKK+ LLK+RD KL EL+ +R K+P+ +WLDDL+ LE ++N
Sbjct:   10 IEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPS-DRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIK---PFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQN-DIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLN 1177          
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0081287)

HSP 1 Score: 1342.02 bits (3472), Expect = 0.000e+0
Identity = 677/1173 (57.72%), Postives = 870/1173 (74.17%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYA--EAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAI-----------------DKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEIN 3542
            IE++YQKK+QLEHILLRPDSY+GS++   +LMWV+D+ +N MV+K+I++ P LYKIFDEILVNA+DNK RD  M TI+IDID + NMV +WNNG+GIPVT+HKE ++Y+PTMIFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS+ F +ET+++EY KSFKQTW NNM    D +I      D+T +TF PDL +F M RLDEDIVAL SRRAYD+AA ++G++VFLNG ++ V+ FKDY+DL+ K+  D +  P+K+V+E   +RWEVA   S +  F QVSFVNSIAT KGG HV+++ D L  ++LE  KKK K         P QV+ ++W+FV CLIENP+FDSQTKENMTL QK FGS+CTL+EKFI+ ++K GIVE +  W  FK Q     K    K +K+K +PKL+DAN AG KNS++CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGKLLNVREA  KQL+ENAEI N+ KIIGLQY +KY   + LKTLRYGK+MIMTDQDQDGSHIKGLLINFIH+ WP LL+  F+E+FITPIVKA K N+ LSFYS+PE  EW+  T   + + +KYYKGLGTSTSKEAKEYF DM+RH I F+Y G  D +SIV+AFSK   + RK WLTN M++ KRR+ELG+ E YLY KGTK IT+ +FIN EL+LFSN DNERSIP L+DG KPGQRKV+F C KRN  +E+KVAQL+GSVAE+SAYHHGE SL  TI+NLAQ+FVG+NNINLL P GQFGTRL+GGKD AS RYI T +S LT+LI+H  D+ +L   +DD Q IEP WY PIIPMVL+NG +GIGTGWS+KI N+NP++++ NL++MING  P  MHPWY+NF G +  V + +Y+  G I  L G  LEI+ELP+ VWT +YKE+V+E   +G +K    I++Y+EY+TD TVRF++   P ++    AE  GF++ FKL T L+ + M  FD+   +R++   I+IL+ ++K+R E Y +R+++L+G L+AQA +L +QARFILEK   K++ EN +   + D+L+   Y  DPVK WQ+ ++   A                   K ++E + D E + K       F YL  M +  LT EKK+ LLK+RD KL EL+ +R K+P+ +WLDDL+ LE ++N
Sbjct:   10 IEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPS-DRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIK---PFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQN-DIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLN 1177          
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136])

HSP 1 Score: 1379.39 bits (3569), Expect = 0.000e+0
Identity = 701/1202 (58.32%), Postives = 900/1202 (74.88%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKG---EEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ--DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+E   M  ++I++ P LYKIFDEILVNA+DNK RD  MTTI+I ID + N + +WNNG+GIPV  HK+ ++Y+P +IFG LLTS+N++D++K+VTGGRNGYGAKLCNIFS +F +ET+ KEY +SFKQTW+ NM    + +I     EDFTCVTF PDL +F+M +LD+DIVAL +RRAYD+A   +G+ V LNGK++ V +F+ YVD Y K  LD     LKVV E+  +RWEV +T S  + F Q+SFVNSIATTKGG H++Y+ DQ+  K++E  KKK K         P QVK ++W+FV  LIENP+FDSQTKENMTL  K FGS+C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+ PESLK+LRYG++MIMTDQDQDGSHIKGLLINF H  WP+LLKH F+E+FITPIVKA KN Q +SFYSIPE  EW+K T     W +KYYKGLGTSTSKEAKEYFSDMERH I F+Y+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E YLYG  T+++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C+KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+N+L P GQFGTR+ GGKD+ASPRYI T LS L KL+F   D ++LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N   P+ M P Y+NF GTI E+ +N+Y+V+GE++ LD  T+EI+ELP+R WT +YKESV+E    G DKTP  I+DYKEY+TD TV+F+V M   K A+AEA G HK FKLQ+ LT ++M+LFD  G +++Y +  EIL+ FF++RL  Y  RK++L+G L A+++KL NQARF+LEKI GK+  EN     LI  LV   Y+SDPV AW K+ EK L  + G   + ++  D    + P+F Y+  M +  LT EK + LLK+RD K  EL+ +++K+ + +W +DL +  +E++ V  Q  ++   G+ V  +VK    K         ET PS F RR+EP+I
Sbjct:   50 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREISFVPGLYKIFDEILVNAADNKQRDKNMTTIKITIDPESNTISVWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDNEKKVTGGRNGYGAKLCNIFSLKFTVETACKEYKRSFKQTWTENMGKSNEAKIKYFDGEDFTCVTFQPDLAKFNMEKLDKDIVALLTRRAYDVAGSCRGVKVILNGKKLPVNSFRSYVDFYVKDKLDETGVALKVVNETVNERWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYIVDQIVGKLIEVVKKKNKAGVSVK---PFQVKNHLWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKILGLQYKKSYDDPESLKSLRYGRIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEEFITPIVKASKNKQEVSFYSIPEFEEWKKHTESYKTWHIKYYKGLGTSTSKEAKEYFSDMERHRIMFKYNGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTSTRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCVKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSSLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGTIHELGQNQYLVSGEVSVLDKNTIEISELPVRTWTQAYKESVLEPMLQGTDKTPPLISDYKEYHTDTTVKFVVRMTEEKLAQAEAAGLHKVFKLQSSLTCNSMVLFDHMGCMKRYESVQEILKEFFELRLHYYKLRKDWLVGSLGAESAKLSNQARFVLEKIEGKLSIENKSKRDLIRMLVQRGYESDPVAAWNKAQEKSLEEEDGGGNDSDSSVDSGSFSGPNFNYILNMPLWCLTKEKVEELLKQRDLKKAELNELQSKTSENLWREDLAVFIEELDRVEEQEKESVNLGKAVK-LVKGKVGKPKVKKMQLEETLPSPFGRRVEPQI 1245          
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453])

HSP 1 Score: 1345.49 bits (3481), Expect = 0.000e+0
Identity = 687/1197 (57.39%), Postives = 888/1197 (74.19%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD++   M  + IT+ P LYKIFDEILVNA+DNK RD  M  I+++ID + N + +WNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ +E  + FKQTW +NM    + +I     +D+TC+TF PDL +F M  LD+D VAL +RRAYDIA  T+G+ V LNGK++ +  F+ YVDLY K  +D    PL VV+E   +RWEV +T S  + F QVSFVNSIATTKGG H +Y+ADQ+ +K++E  KKK K         P QVK +MW+FV CLIENPSFDSQTKENMTL QK+FGS C+L++KF+     CGIVE +  W  FK QA L+KKCS  K TK+K +PKLDDAN AG KNS  CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y  PESLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH  WP+LL+HNF+E+FITPI+KA    Q +SFYSIPE  EW++       W++KYYKGLGTSTSKEAKEYFSDM RH I F+YSG  D ++I LAFSK   +ERKEWLT++M + ++RRE  + E YLYG+ TK +++ +FIN+EL+LFSN+DNERSIPC++DG KPGQRKV+F C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F   D+ +LK   DDN  +EPEWY PIIP+VL NG +GIGTGW+S+IPN++ +++I+N+ RM+NG  P+ M P Y+ F GTI E+ +N+YV NGE+  +D TT+EITELP++ WT +YKE+V+E+  +G +K PA ITDYKEY+TD TVRF+V+M   +  EAEA G HK FKLQ  LT ++M+LFD  G ++KY +  +IL+ FF++R++ Y+ RK++L+G+L A+++KL NQARFILEKI G ++ EN     LI  L    YDSDPVKAW+ + EK    D  +++ +E  + S  PD+ YL  M +  LT EKKD L K+RD K+ EL+ ++ K+P  +W +DL    +E+  V  ++      +    + K      K +K+  ET P+   RR+ P+I
Sbjct:   29 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDTG-MNCRDITFVPGLYKIFDEILVNAADNKQRDKTMNCIKVNIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSLKFTVETACRESKRCFKQTWFDNMGRAGESKIKPFDGDDYTCITFQPDLAKFKMQALDKDTVALLTRRAYDIAGSTKGVRVLLNGKRLPINNFRTYVDLYVKDKVDETGSPLTVVHEMVNERWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKKNKAGVAVK---PFQVKNHMWLFVNCLIENPSFDSQTKENMTLQQKNFGSACSLSDKFVKQANGCGIVESIMNWVKFKAQAQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSNSCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKILGLQYKKNYSDPESLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKASNKKQEISFYSIPEFTEWKEKQSSIKSWKIKYYKGLGTSTSKEAKEYFSDMTRHRIPFKYSGPADDEAITLAFSKKMIEERKEWLTSFMINRRQRREHNLPEEYLYGQETKSLSYHDFINKELVLFSNSDNERSIPCMVDGLKPGQRKVVFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDNLRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVNNGEVAIIDSTTIEITELPVKTWTQTYKENVLEVMLNGTEKVPALITDYKEYHTDTTVRFVVKMTEERLREAEAAGLHKVFKLQNLLTCNSMVLFDHVGSLKKYESVQDILKEFFELRMKYYVLRKDWLVGMLGAESAKLSNQARFILEKIQGTLVIENKPKKELIRMLQQMGYDSDPVKAWKDAQEKEEEEDAEDDDEKEKEDTSG-PDYNYLLSMPMWYLTKEKKDELCKQRDAKMTELNTLKMKAPADLWKEDLAAFTEELERVEQKEKESQNAM---PLVKGKVGKPKVVKVKNETMPTPQGRRVIPRI 1216          
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453])

HSP 1 Score: 416.387 bits (1069), Expect = 6.813e-127
Identity = 226/459 (49.24%), Postives = 316/459 (68.85%), Query Frame = 3
Query: 2310 SLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F   D+ +LK   DDN  +EPEWY PIIP+VL NG +GIGTGW+S+IPN++ +++I+N+ RM+NG  P+ M P Y+ F GTI E+ +N+YV NGE+  +D TT+EITELP++ WT +YKE+V+E+  +G +K PA ITDYKEY+TD TVRF+V+M   +  EAEA G HK FKLQ  LT ++M+LFD  G ++KY +  +IL+ FF++R++ Y+ RK++L+G+L A+++KL NQARFILEKI G ++ EN     LI  L    YDSDPVKAW+ + EK    D  +++ +E  + S  PD+ YL  M +  LT EKKD L K+RD K+ EL+ ++ K+P  +W +DL    +E+  V  ++      +    + K      K +K+  ET P+   RR+ P+I
Sbjct:    2 SLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDNLRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVNNGEVAIIDSTTIEITELPVKTWTQTYKENVLEVMLNGTEKVPALITDYKEYHTDTTVRFVVKMTEERLREAEAAGLHKVFKLQNLLTCNSMVLFDHVGSLKKYESVQDILKEFFELRMKYYVLRKDWLVGMLGAESAKLSNQARFILEKIQGTLVIENKPKKELIRMLQQMGYDSDPVKAWKDAQEKEEEEDAEDDDEKEKEDTSG-PDYNYLLSMPMWYLTKEKKDELCKQRDAKMTELNTLKMKAPADLWKEDLAAFTEELERVEQKEKESQNAM---PLVKGKVGKPKVVKVKNETMPTPQGRRVIPRI 456          
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136])

HSP 1 Score: 127.872 bits (320), Expect = 1.289e-33
Identity = 58/96 (60.42%), Postives = 77/96 (80.21%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRG 383
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+E   M  ++I++ P LYKIFDEILVNA+DNK RD  MTTI+I ID + N + +WNNG+G
Sbjct:   45 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREISFVPGLYKIFDEILVNAADNKQRDKNMTTIKITIDPESNTISVWNNGKG 139          
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293])

HSP 1 Score: 1400.96 bits (3625), Expect = 0.000e+0
Identity = 709/1197 (59.23%), Postives = 898/1197 (75.02%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+++  +  + +T+ P LYKIFDEILVNA+DNK RD  M+ I+I I+ + N V +WNNG+GIPV  HK  +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D  D +I     ED+TC+TF PDL +F M  LD+DIVAL SRRAYD+A  ++G+ VFLNGK++ +  F+ YVDLY K+ LD    PLKV++E    RWE+ +T S  + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E  KKK K         P QVK +MW+FV  LIENP+FDSQTKENMTL  K FGS+CTL+EKFI     CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPI+K  K+ Q LSFYSIPE  EW+  T     W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G  D  +I LAFS+ K D+RKEWLTN+M+D + RR  G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G  P+ M P Y+NF G I E+  N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E   +G +KTP  ITDYKEY+TD TV+F+V++   K  EAEA G HK FKLQTPLT ++M+LFD  G +RKY    +IL  FF +RL+ Y  RK++L+G+L+A+AS+L NQARFILEKI GK++ EN     LI  LV   YDSDPVK+W+++ EK  A +  EEE + ++ ++  PDF YL  M +  L+ EKKD L K+RD K  EL  ++ KSP  +W +DL    +E++ V  ++  E       VVK    +         E  PS   RR+ PK+
Sbjct:   27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEV-NTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217          
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293])

HSP 1 Score: 1397.88 bits (3617), Expect = 0.000e+0
Identity = 709/1197 (59.23%), Postives = 897/1197 (74.94%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+++  +  + +T+ P LYKIFDEILVNA+DNK RD  M+ I+I I+ + N V +WNNG+GIPV  HK  +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D  D +I     ED+TC+TF PDL +F M  LD+DIVAL SRRAYD+A  ++G+ VFLNGK++ +  F+ YVDLY K+ LD    PLKV++E    RWE+ +T S  + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E  KKK K         P QVK +MW+FV  LIENP+FDSQTKENMTL  K FGS+CTL+EKFI     CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPI+K  K+ Q LSFYSIPE  EW+  T     W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G  D  +I LAFS+ K D+RKEWLTN+M+D + RR  G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G  P+ M P Y+NF G I E+  N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E   +G +KTP  ITDYKEY+TD TV+F+V++   K  EAEA G HK FKLQTPLT ++M+LFD  G +RKY    +IL  FF +RL+ Y  RK++L+G+L+A+AS+L NQARFILEKI GK++ EN     LI  LV   YDSDPVK+W+++ EK  A +  EEE + ++  +  PDF YL  M +  L+ EKKD L K+RD K  EL  ++ KSP  +W +DL    +E++ V  ++  E       VVK    +         E  PS   RR+ PK+
Sbjct:   27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEVNTAG-PDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217          
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293])

HSP 1 Score: 1397.88 bits (3617), Expect = 0.000e+0
Identity = 709/1197 (59.23%), Postives = 898/1197 (75.02%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+++  +  + +T+ P LYKIFDEILVNA+DNK RD  M+ I+I I+ + N V +WNNG+GIPV  HK  +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D  D +I     ED+TC+TF PDL +F M  LD+DIVAL SRRAYD+A  ++G+ VFLNGK++ +  F+ YVDLY K+ LD    PLKV++E    RWE+ +T S  + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E  KKK K         P QVK +MW+FV  LIENP+FDSQTKENMTL  K FGS+CTL+EKFI     CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPI+K  K+ Q LSFYSIPE  EW+  T     W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G  D  +I LAFS+ K D+RKEWLTN+M+D + RR  G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G  P+ M P Y+NF G I E+  N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E   +G +KTP  ITDYKEY+TD TV+F+V++   K  EAEA G HK FKLQTPLT ++M+LFD  G +RKY    +IL  FF +RL+ Y  RK++L+G+L+A+AS+L NQARFILEKI GK++ EN     LI  LV   YDSDPVK+W+++ EK  A +  EEE + ++ ++  PDF YL  M +  L+ EKKD L K+RD K  EL  ++ KSP  +W +DL    +E++ V  ++  E       VVK    +         E  PS   RR+ PK+
Sbjct:   27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEV-NTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217          
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293])

HSP 1 Score: 1396.33 bits (3613), Expect = 0.000e+0
Identity = 709/1197 (59.23%), Postives = 897/1197 (74.94%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+++  +  + +T+ P LYKIFDEILVNA+DNK RD  M+ I+I I+ + N V +WNNG+GIPV  HK  +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D  D +I     ED+TC+TF PDL +F M  LD+DIVAL SRRAYD+A  ++G+ VFLNGK++ +  F+ YVDLY K+ LD    PLKV++E    RWE+ +T S  + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E  KKK K         P QVK +MW+FV  LIENP+FDSQTKENMTL  K FGS+CTL+EKFI     CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPI+K  K+ Q LSFYSIPE  EW+  T     W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G  D  +I LAFS+ K D+RKEWLTN+M+D + RR  G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G  P+ M P Y+NF G I E+  N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E   +G +KTP  ITDYKEY+TD TV+F+V++   K  EAEA G HK FKLQTPLT ++M+LFD  G +RKY    +IL  FF +RL+ Y  RK++L+G+L+A+AS+L NQARFILEKI GK++ EN     LI  LV   YDSDPVK+W+++ EK  A +  EEE + ++  +  PDF YL  M +  L+ EKKD L K+RD K  EL  ++ KSP  +W +DL    +E++ V  ++  E       VVK    +         E  PS   RR+ PK+
Sbjct:   27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEVNTAG-PDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217          
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293])

HSP 1 Score: 1394.02 bits (3607), Expect = 0.000e+0
Identity = 709/1207 (58.74%), Postives = 899/1207 (74.48%), Query Frame = 3
Query:   66 ESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +S N+R+   +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+++  +  + +T+ P LYKIFDEILVNA+DNK RD  M+ I+I I+ + N V +WNNG+GIPV  HK  +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D  D +I     ED+TC+TF PDL +F M  LD+DIVAL SRRAYD+A  ++G+ VFLNGK++ +  F+ YVDLY K+ LD    PLKV++E    RWE+ +T S  + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E  KKK K         P QVK +MW+FV  LIENP+FDSQTKENMTL  K FGS+CTL+EKFI     CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPI+K  K+ Q LSFYSIPE  EW+  T     W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G  D  +I LAFS+ K D+RKEWLTN+M+D + RR  G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G  P+ M P Y+NF G I E+  N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E   +G +KTP  ITDYKEY+TD TV+F+V++   K  EAEA G HK FKLQTPLT ++M+LFD  G +RKY    +IL  FF +RL+ Y  RK++L+G+L+A+AS+L NQARFILEKI GK++ EN     LI  LV   YDSDPVK+W+++ EK+        E ++     + PDF YL  M +  L+ EKKD L K+RD K  EL  ++ KSP  +W +DL    +E++ V  ++  E       VVK    +         E  PS   RR+ PK+
Sbjct:   19 KSDNKRL--SVERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKVFIC----VEFRDRSLLPSGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1214          
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2a (topoisomerase (DNA) II alpha [Source:MGI Symbol;Acc:MGI:98790])

HSP 1 Score: 1389.79 bits (3596), Expect = 0.000e+0
Identity = 693/1155 (60.00%), Postives = 882/1155 (76.36%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKL----LAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3548
            IE++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   +  +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV  HK  ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+S+EY K FKQTW +NM    D E+   + ED+TC+TF PDL +F M  LD+DIVAL  RRAYDIA  T+ + VFLNG  + VK F+ YVDLY K  +D     LKV++E    RWEV +T S    F Q+SFVNSIAT+KGG HV+Y+ADQ+ +K+++  KKK K           QVK +MWIFV  LIENP+FDSQTKENMTL  K FGS C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + YE  +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LL+H F+E+FITPIVK  KN Q ++FYS+PE  EW+  TP   +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED  +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F   D+  L+ L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G  P+ M P Y+NF GTI E+  N+YV+NGE+  LD TT+EI+ELPIR WT +YKE V+E   +G +KTP+ ITDY+EY+TD TV+F+++M   K AEAE VG HK FKLQ+ LT ++M+LFD  G ++KY+  ++IL  FF++RL+ Y  RK +LLG+L A++SKL NQARFILEKI+GK++ EN     LI  L+   YDSDPVKAW+++ +K+       +   E +  D      P F YL  M +  LT EKKD L K+R+EK +EL+ ++ KSP  +W +DL +  +E+ VV
Sbjct:   29 IERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMS-ERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVV 1178          
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791])

HSP 1 Score: 1385.16 bits (3584), Expect = 0.000e+0
Identity = 703/1201 (58.53%), Postives = 896/1201 (74.60%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDS---NKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE-PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   +LMWV+D++   M  +++T+ P LYKIFDEILVNA+DNK RD  MT I++ ID + N++ IWNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    + +I     ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A   +G+ V  NGK++ V  F+ YVDLY K  LD     LKV++E   +RW+V +T S  + F Q+SFVNSIATTKGG HV+Y+ DQ+ +K++E  KKK K         P QVK ++W+F+ CLIENP+FDSQTKENMTL  K FGS+C L+EKF    + CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE  EW+K       W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E +LYG  TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM+ G  P  M P Y+NF GTI E+ +N+Y V+GEI  +D  T+EITELP+R WT  YKE V+E   +G DKTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M+LFD  G ++KY    +IL+ FF +RL  Y  RK +L+G+L A+++KL NQARFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK    +  + ++ +   DS   + PDF Y+  M + +LT EK + L+K+RD K +E++ ++ KSP  +W +DL    +E++ V  Q+  +    +    +K    K         ET PS + RRI P+I
Sbjct:   39 VERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEI 1234          
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791])

HSP 1 Score: 184.111 bits (466), Expect = 4.742e-52
Identity = 84/156 (53.85%), Postives = 116/156 (74.36%), Query Frame = 3
Query: 2571 INGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDE 3038
            ING +GIGTGW+ K+PN++ +++++N++RM+ G  P  M P Y+NF GTI E+ +N+Y V+GEI  +D  T+EITELP+R WT  YKE V+E   +G DKTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M   +E
Sbjct:    2 INGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMAAEEE 157          
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791])

HSP 1 Score: 66.6254 bits (161), Expect = 8.586e-11
Identity = 49/124 (39.52%), Postives = 76/124 (61.29%), Query Frame = 3
Query: 3180 RFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDS---NKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEIN 3542
            RFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK    +  + ++ +   DS   + PDF Y+  M + +LT EK + L+K+RD K +E++ ++ KSP  +W +DL    +E++
Sbjct:    1 RFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELD 124          
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q02880|TOP2B_HUMAN (DNA topoisomerase 2-beta OS=Homo sapiens OX=9606 GN=TOP2B PE=1 SV=3)

HSP 1 Score: 1392.1 bits (3602), Expect = 0.000e+0
Identity = 706/1203 (58.69%), Postives = 898/1203 (74.65%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP-GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   M  +++T+ P LYKIFDEILVNA+DNK RD  MT I++ ID + N++ IWNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    + +I     ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A   +G+ V  NGK++ V  F+ YVDLY K  LD     LKV++E   +RW+V +T S  + F Q+SFVNSIATTKGG HV+Y+ DQ+  K++E  KKK K         P QVK ++W+F+ CLIENP+FDSQTKENMTL  K FGS+C L+EKF    + CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE  EW+K       W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E +LYG  TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G  P  M P Y+NF GTI E+ +N+Y V+GEI  +D  T+EITELP+R WT  YKE V+E   +G DKTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M+LFD  G ++KY    +IL+ FF +RL  Y  RK +L+G+L A+++KL NQARFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK  A ++ E +NQ D   S+      PDF Y+  M + +LT EK + L+K+RD K +E++ ++ KSP  +W +DL    +E++ V +Q+  +    +    +K    K         ET PS + RRI P+I
Sbjct:   51 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEI 1246          
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|P41515|TOP2A_CRIGR (DNA topoisomerase 2-alpha OS=Cricetulus griseus OX=10029 GN=TOP2A PE=1 SV=1)

HSP 1 Score: 1392.1 bits (3602), Expect = 0.000e+0
Identity = 698/1154 (60.49%), Postives = 885/1154 (76.69%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW---QKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3548
            IE++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   +  +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV  HK  ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS+ F +ET+SKEY K FKQTW +NM    D E+     ED+TC+TF PDL +F M  LD+DIVAL  RRAYDIA  T+ + VFLNG ++ VK F+ YVD+Y K  LD     LKVV+E    RWEV +T S  + F Q+SFVNSIAT+KGG HV+Y+ADQ+ +K+++  KKK K           QVK +MWIFV  LIENP+FDSQTKENMTL  K FGS C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K  ++K +PKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + YE  +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LL+H F+E+FITPIVK  KN Q L+FYS+PE  EW+  TP   +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED  +I LAFSK + D+RKEWLT++MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS LT+L+F   D+  LK L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G  P+ M P Y+NF GTI E+  N+YV+NGE+  L+ TT+EI+ELPIR WT +YKE V+E   +G +KTP  ITDY+EY+TD TV+F+++M   K AEAE VG HK FKLQT LT ++M+LFD  G ++KY+  ++IL+ FF++RL+ Y  RK +LLG+L A+++KL NQARFILEKI+GK+I EN     LI  L+   YDSDPVKAW   Q+ V      ++ + EN + + +S  P F YL  M +  LT EKKD L K+R+EK +EL+ ++NKSP  +W +DL +  +E+ VV
Sbjct:   29 IERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTRFTVETASKEYKKMFKQTWMDNMGRAGDMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNALKVVHEQVNPRWEVCLTMS-EKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQELAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLTRLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILKDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENSDSVAESG-PTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKNKSPSDLWKEDLAVFIEELEVV 1176          
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q01320|TOP2A_MOUSE (DNA topoisomerase 2-alpha OS=Mus musculus OX=10090 GN=Top2a PE=1 SV=2)

HSP 1 Score: 1389.79 bits (3596), Expect = 0.000e+0
Identity = 693/1155 (60.00%), Postives = 882/1155 (76.36%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKL----LAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3548
            IE++YQKKTQLEHILLRPD+Y+GS++   + MWV+D++   +  +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV  HK  ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+S+EY K FKQTW +NM    D E+   + ED+TC+TF PDL +F M  LD+DIVAL  RRAYDIA  T+ + VFLNG  + VK F+ YVDLY K  +D     LKV++E    RWEV +T S    F Q+SFVNSIAT+KGG HV+Y+ADQ+ +K+++  KKK K           QVK +MWIFV  LIENP+FDSQTKENMTL  K FGS C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + YE  +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LL+H F+E+FITPIVK  KN Q ++FYS+PE  EW+  TP   +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED  +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F   D+  L+ L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G  P+ M P Y+NF GTI E+  N+YV+NGE+  LD TT+EI+ELPIR WT +YKE V+E   +G +KTP+ ITDY+EY+TD TV+F+++M   K AEAE VG HK FKLQ+ LT ++M+LFD  G ++KY+  ++IL  FF++RL+ Y  RK +LLG+L A++SKL NQARFILEKI+GK++ EN     LI  L+   YDSDPVKAW+++ +K+       +   E +  D      P F YL  M +  LT EKKD L K+R+EK +EL+ ++ KSP  +W +DL +  +E+ VV
Sbjct:   29 IERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMS-ERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVV 1178          
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q64511|TOP2B_MOUSE (DNA topoisomerase 2-beta OS=Mus musculus OX=10090 GN=Top2b PE=1 SV=2)

HSP 1 Score: 1385.16 bits (3584), Expect = 0.000e+0
Identity = 703/1201 (58.53%), Postives = 896/1201 (74.60%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDS---NKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE-PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   +LMWV+D++   M  +++T+ P LYKIFDEILVNA+DNK RD  MT I++ ID + N++ IWNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    + +I     ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A   +G+ V  NGK++ V  F+ YVDLY K  LD     LKV++E   +RW+V +T S  + F Q+SFVNSIATTKGG HV+Y+ DQ+ +K++E  KKK K         P QVK ++W+F+ CLIENP+FDSQTKENMTL  K FGS+C L+EKF    + CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+  ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE  EW+K       W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E +LYG  TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM+ G  P  M P Y+NF GTI E+ +N+Y V+GEI  +D  T+EITELP+R WT  YKE V+E   +G DKTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M+LFD  G ++KY    +IL+ FF +RL  Y  RK +L+G+L A+++KL NQARFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK    +  + ++ +   DS   + PDF Y+  M + +LT EK + L+K+RD K +E++ ++ KSP  +W +DL    +E++ V  Q+  +    +    +K    K         ET PS + RRI P+I
Sbjct:   39 VERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEI 1234          
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|O42131|TOP2B_CHICK (DNA topoisomerase 2-beta OS=Gallus gallus OX=9031 GN=TOP2B PE=2 SV=1)

HSP 1 Score: 1383.24 bits (3579), Expect = 0.000e+0
Identity = 704/1201 (58.62%), Postives = 893/1201 (74.35%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQE---DIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP-GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   +LMWV+D++   M  +++T+ P LYKIFDEILVNA+DNK RD  MT I+I ID + N++ IWNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    +P+I     +D+TC+TF PDL +F M  LD+DIV+L +RRAYD+A   +G+ V LNGK++ V  F+ YVDLY K  LD     LKV++E   +RW+V +T S  + F Q+SFVNSIATTKGG HV+Y+ DQ+  K++E  KKK K         P QVK ++W+FV CLIENPSFDSQTKENMTL  K FGS+C L+EKF    + CGI+E +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+SL CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + YE PESLK+LRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE  EW+K       W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E +LYG  TK++T+ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+NLL P GQFGTRL GGKD+ASPRYI T LS L +L+F   D+ +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ K+PN++ +++++N++RM++G  P  M P Y+NF GTI E+ +N+YVV+GEI  +D  T+EITELP+R WT  YKE V+E   +G +KTPA I+DYKEY+TD TV+F+V+M   K A+AEA G HK FKLQT LT ++M+LFD  G ++KY    +IL+ FF +RL  Y  RK +L+G+L A+++KL NQARFILEKI GK+  EN     LI  LV   Y+SDPVKAW+++ EK    +  +  N +       ++ PDF Y+  M + +LT EK + L+K RD K +EL+ ++ KS   +W +DL    +E+  V  Q+  +    +V   +K    K         ET PS F RRI P+I
Sbjct:   56 VERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMENLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQI 1251          
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A2R2MQU0 (DNA topoisomerase 2 OS=Lingula unguis OX=7574 GN=LOC106174550 PE=3 SV=1)

HSP 1 Score: 1446.03 bits (3742), Expect = 0.000e+0
Identity = 728/1206 (60.36%), Postives = 922/1206 (76.45%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK------PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNT-VVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEE 3692
            +E++YQKK+QLEHILLRPD+Y+GS++K  + MWV ++E + M+++ +T+ P LYKIFDEILVNA+DNK RD KMTTI+IDIDS+ N V+IWNNG+GIPV  HK  +V +PT+IFG LLTS+N+ND++++VTGGRNGYGAKLCNIFS++FV+ETSSK+ G +FKQTW++NM    + +I+  A ED+TC+TF PDL +F M  LD+DIV LFSRRAYDIAA T+G+ VFLNGK++ + +F+ YVDL+ K   D N +PL VV+E    RWEVAVT S +  F Q SFVNSIATTKGG H++Y  DQ+  K+ +  KKK      AVK  P Q+K +MW+FV CLI NP+FDSQTKENMTL  K FGS+C LN+KFI  V+KCGIVE +  W  FK Q  L+KKC  +K +KLK +PKLDDAN AGTKNS  CTLILTEGDSAK+LAV+GL+VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI N+IKI+GLQY  KY+ PE+LKTLRYGK+MIMTDQDQDGSHIKGLL+NFIH  WP+LLKHNFVE+FITPIVK  K  +  +FYS+PE  EW++ TP  + +R KYYKGLGTSTSKEAKEYFSDMERH I F+YSG  D  SI LAFSK K +ERK+WL+N+MED KRR ELG+ +VYLYGK T++IT+ +FIN+ELILFSN DNERSIP L+DGFKPGQRKV+F CLKRNL KE+KVAQLAGSV+E+SAYHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKD+ASPRYI T LS L + +F+  DE + K   DDN  +EP++Y PI+PMVL+NG +GIGTGWS+KIPN++ ++++ NLKR+I+G  P  M P ++NF GTI E    + + +GE++ LD  T+EITELPIRVWT +YKESV+E    G DKTPA I+DYKEY+TD TV+F+V+M   K A+AE VG HKFFKLQT ++ SNM+LFD+ G ++KY+   ++L  F+ VR+E YIKRK+YL G+LSA+A KL+NQARFI EKI+G++  EN     LI  LV   YDSDP+KAW+++ +KL   D+G  E + D E S        PDF Y+  + + +L+ EKK+ LLK+RD K +EL  +R K+P  +W DDLE   +E+  V  ++  +    VN  VVK    +         ET PS   RR+EP+I++
Sbjct:   65 VERIYQKKSQLEHILLRPDTYIGSVEKVTQPMWVINEEADRMIQRDLTFVPGLYKIFDEILVNAADNKQRDPKMTTIKIDIDSENNRVKIWNNGKGIPVIEHKTEKVMVPTLIFGHLLTSSNYNDEEQKVTGGRNGYGAKLCNIFSNKFVVETSSKDEGHAFKQTWTHNMGKATEAKITPCASEDYTCITFEPDLAKFKMDSLDKDIVDLFSRRAYDIAASTKGVKVFLNGKKLPINSFRSYVDLFLKDKFDDNGQPLNVVHEVINPRWEVAVTLS-DSGFKQASFVNSIATTKGGRHIDYFVDQVVNKLADVVKKKQGKGGMAVK--PFQIKNHMWVFVNCLIVNPTFDSQTKENMTLQAKSFGSKCELNDKFIQQVSKCGIVESILSWVKFKTQTQLNKKCHASKHSKLKGIPKLDDANDAGTKNSAHCTLILTEGDSAKTLAVAGLSVIGRDQYGVFPLRGKVLNVREATHKQIMENAEINNLIKIVGLQYKNKYDSPETLKTLRYGKIMIMTDQDQDGSHIKGLLVNFIHHNWPSLLKHNFVEEFITPIVKVTKGKEEKAFYSLPEFEEWKEATPNWHTYRCKYYKGLGTSTSKEAKEYFSDMERHRIPFKYSGPSDDASITLAFSKKKIEERKQWLSNFMEDRKRRAELGLPDVYLYGKDTRHITYNDFINKELILFSNMDNERSIPSLIDGFKPGQRKVMFTCLKRNLQKEIKVAQLAGSVSEMSAYHHGEMSLMGTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDAASPRYIFTALSPLARAVFNAHDEPLYKYCYDDNLKVEPQFYVPILPMVLVNGAEGIGTGWSTKIPNYDIREIVSNLKRLIDGEEPRPMIPAFKNFIGTI-EDHSGETICHGEVSILDDETIEITELPIRVWTQNYKESVLEPMLQGTDKTPAVISDYKEYHTDVTVKFVVKMTAEKLAQAEQVGLHKFFKLQTSIS-SNMVLFDKNGCLKKYDRAEDVLREFYDVRMEYYIKRKDYLEGLLSAEALKLENQARFICEKIDGEISIENKPKKELIQLLVRRGYDSDPLKAWREAQDKLAEADRGGVETESDDEQSEASSTAGGPDFNYILNLPLWSLSKEKKEELLKQRDAKQEELRALRRKTPSCLWKDDLEKFLEELEKVEQKEREDAAAGVNPGVVKTAKGRAKPKKIHIEETKPSPMGRRVEPRIDQ 1265          
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A094ZNL1 (DNA topoisomerase 2 OS=Schistosoma haematobium OX=6185 GN=MS3_03959 PE=3 SV=1)

HSP 1 Score: 1442.17 bits (3732), Expect = 0.000e+0
Identity = 761/1367 (55.67%), Postives = 982/1367 (71.84%), Query Frame = 3
Query:   66 ESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEEN------QEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTV---------VXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGD-NNSENDEELTLAQRLAKTHGSEAFKGLIGKG---DKPTKTNKTNILIEKNAKVSP-------GNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMS 4088
            E++ +R+   +E++YQKKTQLEHILLRPDSY+GS  K+ + MWV D+ +  MV + IT++P LYKIFDEILVNA+DNK+RD  M+ I+IDI+   NMVRIWN+G+GIPV  HK    Y+PT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +F++ETSSKEY KSF+Q W  NM    DP+I     +DFTC+TFYPDL RF M  L++D V+LF+RRAYD+AA   G+ VFLNGK++ +K+F DYV+L+ K   D + + +KVVYE+   RW+VAV  S N  F QVSFVNSIATTKGGTH+NY++DQ+  K++E  KKK       +K  P Q+K +MW+FV CLIENP+FDSQTKENMTL  K FGS CTL+EKFI   +K GIVE +  W  FK    +DK+C K+K +KLK +PKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI  +IKI+GLQY  KYE  ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H  WPNLLKHN VE+FITPIVK FK  Q  SFYS+PE  EWQK TP  + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED  SI LAF K+K  +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L  L DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N  ++I+NL RM++G  P +M P +R FTGTI ++  N+YV  GE+  LD  TLEITELPIRVWT +YKESV+E   +G++K PA ITDYKEY+TD TVRF+V+M+P K  EAE+ G HKFFKLQT ++  +M+ FD  G ++ Y N + I+  F+++RL  Y KRK YL G+LSA+A KL+NQARF+LEKI   ++ EN     LI  L  A YDSDPVKAW++S++K  A+ + EE        Q +  ++ +PD+ Y+  M + +LT E+KD LL +RD K KEL I+++KSP  +W +DL+ LE+E   V  +  ++    +  V                  K+ K+A ET P    RRI P ++ + +KK+    +K+ K   +  +   ++N         + EA D  N  NDE   LAQRL  +  ++  K    +G    +   T++  +L   +  +SP       GN +++ S  +   S +D GDS ++Q  + ++   +S
Sbjct:   24 EAVKKRL--SVERIYQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVSRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVENMTKTTDPKILADKGDDFTCITFYPDLARFGMNELEQDTVSLFTRRAYDMAATCNGVKVFLNGKRLPIKSFMDYVNLFIKPNKD-DGDSVKVVYEAVNPRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMEIVKKKSGKSGVQIK--PFQIKNHMWLFVNCLIENPTFDSQTKENMTLQTKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESVDTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEYSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGTEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGNNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVVKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEMVIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAWKESIDKAAAVQEQEESRTDEGAPQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAVEKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVPTVDADMMKKVEGNKKKRNKTENDLNEGNELDN---------SFEANDEENDGNDEPKPLAQRLGASEKTDVQKTATTRGRGKGRTVPTSRPKLLKPSSPNISPIKRRNVGGNAKRKRSSRMPWESGSDTGDS-ESQVSIDSDNDHIS 1374          
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A3Q0KH18 (DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 1436.01 bits (3716), Expect = 0.000e+0
Identity = 756/1342 (56.33%), Postives = 969/1342 (72.21%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEE------NQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ---------DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKG---DKPTKTNKTNILIEKNAKVSP-------GNKRKRDSGEIIELSDNDDGDS 4043
            +E++YQKKTQLEHILLRPDSY+GS  K+ + MWV D+ +  MV + IT++P LYKIFDEILVNA+DNK+RD  M+ I+IDI+   NMVRIWN+G+GIPV  HK    Y+PT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +F++ETSSKEY KSF+Q W +NM    DP+I     EDFTC+TFYPDL RF M  L++D V+LF+RRAYD+AA   GI VFLNGK++ +K F DYV+L+ K   D + + +KVVYE+  +RW+VAV  S N  F QVSFVNSIATTKGGTH+NY++DQ+  K+++  KKK       +K  P Q+K +MW+FV CLIENP+FDSQTKENMTL  K FGS CTL+EKFI   +K GIVE +  W  FK    +DK+C K+K +KLK +PKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI  +IKI+GLQY  KYE  ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H  WPNLLKHN VE+FITPIVK FK  Q  SFYS+PE  EWQK TP  + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED  SI LAF K+K  +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L  L DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N  ++I+NL RM++G  P +M P +R FTGTI ++  N+YV  GE+  LD  TLEITELPIRVWT +YKESV+E   +G++K PA ITDYKEY+TD TVRF+++M+P K  EAE+ G HKFFKLQT ++  +M+ FD  G ++ Y N I I+  F+++RL  Y KRK YL G+LSA+A KL+NQARF+LEKI   ++ EN     LI  L  A YDSDPVKAW++S++K  A+ + EE       +Q +  ++ +PD+ Y+  M + +LT E+KD LL +RD K KEL I+++KSP  +W +DL+ LE+E   V  +         + ++    + +          K+ K+A ET P    RRI P ++ + +KK+    +K+ K   +  +   ++N         + EA D  N  NDE   LAQRL  +  ++  K    +G    +   T++  +    +   SP       GN +++ S  +   S +D GDS
Sbjct:   32 VERIYQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVNRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVDNMTKTSDPKILADKGEDFTCITFYPDLSRFGMNELEQDTVSLFTRRAYDMAATCNGIKVFLNGKRLPIKNFVDYVNLFIKPNKD-DADSVKVVYEAVNQRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMDIVKKKSGKSGVQIK--PFQIKNHMWLFVNCLIENPTFDSQTKENMTLQIKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESADTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEHSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGIEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGSNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVIKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEIIIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAWKESIDKAAALQEQEEARTDEAVSQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAADKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVPTVDADIMKKVEGNKKKRNKTENDPNEGNELDN---------SFEANDEENDGNDEPKPLAQRLGVSEKTDIQKTATTRGRGKGRTVPTSRPKLPKPSSPNNSPIKRKNMGGNAKRKRSSRMPWESGSDTGDS 1360          
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: G4VPA9 (DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183 GN=Smp_065840 PE=3 SV=1)

HSP 1 Score: 1434.47 bits (3712), Expect = 0.000e+0
Identity = 756/1342 (56.33%), Postives = 969/1342 (72.21%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEE------NQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ---------DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKG---DKPTKTNKTNILIEKNAKVSP-------GNKRKRDSGEIIELSDNDDGDS 4043
            +E++YQKKTQLEHILLRPDSY+GS  K+ + MWV D+ +  MV + IT++P LYKIFDEILVNA+DNK+RD  M+ I+IDI+   NMVRIWN+G+GIPV  HK    Y+PT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +F++ETSSKEY KSF+Q W +NM    DP+I     EDFTC+TFYPDL RF M  L++D V+LF+RRAYD+AA   GI VFLNGK++ +K F DYV+L+ K   D + + +KVVYE+  +RW+VAV  S N  F QVSFVNSIATTKGGTH+NY++DQ+  K+++  KKK       +K  P Q+K +MW+FV CLIENP+FDSQTKENMTL  K FGS CTL+EKFI   +K GIVE +  W  FK    +DK+C K+K +KLK +PKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI  +IKI+GLQY  KYE  ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H  WPNLLKHN VE+FITPIVK FK  Q  SFYS+PE  EWQK TP  + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED  SI LAF K+K  +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L  L DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N  ++I+NL RM++G  P +M P +R FTGTI ++  N+YV  GE+  LD  TLEITELPIRVWT +YKESV+E   +G++K PA ITDYKEY+TD TVRF+++M+P K  EAE+ G HKFFKLQT ++  +M+ FD  G ++ Y N I I+  F+++RL  Y KRK YL G+LSA+A KL+NQARF+LEKI   ++ EN     LI  L  A YDSDPVKAW++S++K  A+ + EE       +Q +  ++ +PD+ Y+  M + +LT E+KD LL +RD K KEL I+++KSP  +W +DL+ LE+E   V  +         + ++    + +          K+ K+A ET P    RRI P ++ + +KK+    +K+ K   +  +   ++N         + EA D  N  NDE   LAQRL  +  ++  K    +G    +   T++  +    +   SP       GN +++ S  +   S +D GDS
Sbjct:   32 VERIYQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVNRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVDNMTKTSDPKILADKGEDFTCITFYPDLSRFGMNELEQDTVSLFTRRAYDMAATCNGIKVFLNGKRLPIKNFVDYVNLFIKPNKD-DADSVKVVYEAVNQRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMDIVKKKSGKSGVQIK--PFQIKNHMWLFVNCLIENPTFDSQTKENMTLQIKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESADTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEHSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGIEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGSNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVIKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEIIIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAWKESIDKAAALQEQEEARTDEAVSQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAADKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVPTVDADIMKKVEGNKKKRNKTENDPNEGNELDN---------SFEANDEENDGNDEPKPLAQRLGVSEKTDIQKTATTRGRGKGRTVPTSRPKLPKPSSPNNSPIKRKNMGGNAKRKRSSRMPWESGSDTGDS 1360          
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A0X3PUD6 (DNA topoisomerase 2 OS=Schistocephalus solidus OX=70667 GN=TR96187 PE=3 SV=1)

HSP 1 Score: 1431.77 bits (3705), Expect = 0.000e+0
Identity = 717/1180 (60.76%), Postives = 893/1180 (75.68%), Query Frame = 3
Query:   75 NERVPED-----------IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANE--EPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQ---------------EDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILE 3530
            N  VPED           +E++YQKKTQLEHIL+RPD+Y+GS+++   LMW++D EK  M++K+ITY P LYKI+DEILVNA+DNK+RD  M  I+IDI ++ N++RIWNNG GIPV  H+  ++++P++IFG LLTS+N++D +K+VTGGRNG+GAKLCNIFS +FV+ETSSKE+ K FKQTW +NM    +P I+ +A EDFTC++FYPDL RF MT L+ D VALF RRAYDIAA T+G+ V LNG ++ +K FKDYVDLY K   D  E  E + VVYE    RWEVAV  S N  F QVSFVNSIATTKGG H++Y++DQ+A K+++  KKK       VK    Q++ +MW+FV CLIENPSFDSQTKE MTL  K FGS C L+EKFI+ V++ GIVE +  W  FK Q  +DK+C K+K  KLK +PKLDDAN AGTKNS  CTLILTEGDSAK+LAV+GL+V+GRD YGVFPLRGKLLNVREA  KQ+ ENAEI N+IKI+GLQY  KYE PES+ TLRYGK+MIMTDQDQDGSHIKGLLI+FIH  WP LL HNF+E+FITPIVK FK  Q L+FYSIPE  EWQK T   + WR+KYYKGLGTSTSKEAK+YFSDM RH I+FRYSG ED  SI LAF+K K+D+RK WLT WM + KRR ELG+ E YLYG GT  IT+ +F+++ELILFSN DNERSIP L+DG KPGQRKVL+ CLKRNLVKE+KVAQL+GSVAE+S+YHHGEASLM TII LAQDFVGSNN+NLLMP+GQFGTRL+GGKD+ASPRYI T LS  T+ IF   D+A+L  L +DNQ IEP WY P+IPMVLING DGIGTGW +KIPN++  +VI NL+RMI+G  P+ M P +R+F G I E+ +N+YVV GEI  LD  T+EITELP+RVWT SYKE V+E+  +G++K PA I DYKEY+TD TVRF+V M P K  EAEA+G HKFFKL T +T ++M+LFD  G +++Y +  EIL+ FF++RLE Y KRK+YL G+LSA+A +L+NQARF+LEKI GK+  EN     LI  L    Y+ DPV AW++ ++KL AI+     ++               +    S   D+ Y+  M + +LT E+KD LL +RD+K  EL  + +KS +++W DDL+ LE
Sbjct:    8 NGDVPEDGIVDPAKKRLSVERIYQKKTQLEHILIRPDTYIGSVERATTLMWLYDKEKQAMIQKEITYTPGLYKIYDEILVNAADNKVRDPSMNCIKIDIKAEDNLIRIWNNGAGIPVVHHRVEKMFVPSLIFGHLLTSSNYDDTEKKVTGGRNGFGAKLCNIFSRKFVVETSSKEHKKCFKQTWIDNMTKTSEPRITASAGEDFTCISFYPDLARFGMTELEADTVALFERRAYDIAASTRGVKVILNGVRVPIKNFKDYVDLYLK---DKGEDGEAVPVVYECVNPRWEVAVAAS-NLGFQQVSFVNSIATTKGGKHIDYISDQVANKLVDVVKKKSGKSGVTVKSF--QIRSHMWVFVNCLIENPSFDSQTKEFMTLQSKSFGSTCVLSEKFINQVSRSGIVENVLSWVRFKAQEKMDKQCHKSKHVKLKGIPKLDDANDAGTKNSAACTLILTEGDSAKTLAVAGLSVVGRDRYGVFPLRGKLLNVREASSKQIMENAEINNLIKILGLQYKNKYETPESVATLRYGKIMIMTDQDQDGSHIKGLLISFIHHNWPKLLHHNFLEEFITPIVKVFKGKQELAFYSIPEFEEWQKTTSNWHTWRVKYYKGLGTSTSKEAKDYFSDMTRHRIRFRYSGPEDDASITLAFNKNKSDDRKSWLTTWMNERKRRVELGLPEDYLYGSGTHAITYSDFVHKELILFSNMDNERSIPSLVDGLKPGQRKVLYTCLKRNLVKEIKVAQLSGSVAEMSSYHHGEASLMGTIIGLAQDFVGSNNLNLLMPHGQFGTRLSGGKDAASPRYIFTSLSPFTRKIFSERDDAILHYLFEDNQRIEPYWYIPVIPMVLINGADGIGTGWGTKIPNYDVMEVISNLRRMIDGADPLPMLPSFRDFRGVIQELGDNRYVVFGEIAILDEQTVEITELPVRVWTQSYKEGVLEVMLNGSEKVPASILDYKEYHTDATVRFVVRMTPEKLREAEAIGLHKFFKLSTAMTTNSMVLFDHMGCVKRYASVSEILKEFFELRLEWYNKRKSYLEGMLSAEARRLENQARFVLEKIQGKISIENRAKRDLIRLLKEHNYEPDPVHAWKECIDKLAAIEDARAASRAAGEGEEEDSEEQGGDASTSSAAADYNYILGMPLWSLTKERKDDLLAQRDKKQAELTELLSKSARRLWRDDLDELE 1181          
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136])

HSP 1 Score: 1389.79 bits (3596), Expect = 0.000e+0
Identity = 712/1207 (58.99%), Postives = 899/1207 (74.48%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIE--------DSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ--DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD+E   M  ++ITY P LYKIFDEILVNA+DNK RD  M++I+I ID + N V IWNNG+GIPV  HK+ ++Y+P +IFG LLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    DP+I     +DFTCVTF PDL +F M +LD+DIVAL +RRAYD+A   +G+ VFLNGK++ V  F+ YVDLY K  LD     LKVV ES  +RWEV +T S  + F Q+SFVNSIATTKGG H++Y+ DQ+ +K++E  KKK K         P QVK ++W+FV  LIENP+FDSQTKENMTL  K FGS+C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+ PESLK+LRYGK+MIMTDQDQDGSHIKGLLINF H  WP+LLKH F+E+FITPIVKA KN Q + FYSIPE  EW+K T     W +KYYKGLGTSTSKEAKEYF+DMERH I F+Y G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E YLYG  T+++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C+KRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+N+L P GQFGTR+ GGKD+ASPRYI T LS L KL+F   D  +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM++   P+ M P Y+NF G I E+ +N+YVV+GEI+ LD  T+EITELPIR WT +YKESV+E    G +KTPA I DYKEY+TD TV+F+V M   K A+AEA G HK FKLQ+ LT ++M+LFD  G +++Y +  +IL+ FF++RL  Y  RK++L+G L A+++KL NQARF+LEKI GK+  EN     LI  LV   Y+SDPV AW ++ EK L     EEE+++  E         S+ P+F Y+  M +  LT EK D LLK+RD K  EL+ ++ K  + +W +DL +  +E++ V  Q  +++  G+ V  +VK    K         ET PS F RR+EP+I
Sbjct:   50 VERVYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREITYVPGLYKIFDEILVNAADNKQRDKNMSSIKITIDPESNTVSIWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWQNNMTKTSDPKIKYFDGDDFTCVTFQPDLSKFKMEKLDKDIVALLTRRAYDVAGSCKGVKVFLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVVNESVNERWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVSKLIEVVKKKNKAGVTVK---PFQVKNHIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEEFITPIVKASKNKQEIPFYSIPEFDEWKKQTENYKTWHIKYYKGLGTSTSKEAKEYFADMERHRIMFKYGGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTATRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCMKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLDHQDPLPMLPSYKNFKGVIHELGQNQYVVSGEISVLDKNTIEITELPIRTWTQAYKESVLEPMLQGTEKTPALINDYKEYHTDTTVKFVVRMTEEKLAQAEAAGLHKVFKLQSSLTCNSMVLFDHMGCMKRYESVQDILKEFFELRLHYYKLRKDWLVGSLGAESAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQRGYESDPVAAWNRAQEKAL-----EEEDRDGNESDSSIDSGSSSGPNFNYILNMPLWCLTKEKVDELLKQRDLKKAELNELQRKCSEDLWREDLAVFVEELDRVEEQERESASQGKGVK-LVKGKVGKPKVKKIQLEETLPSPFGRRVEPQI 1245          
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453])

HSP 1 Score: 1339.32 bits (3465), Expect = 0.000e+0
Identity = 680/1197 (56.81%), Postives = 878/1197 (73.35%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD++   +  + IT+ P LYKIFDEILVNA+DNK RD+ M  I+I+IDS+ N + +WNNG+GIPV  HK  +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+  E   SFKQTW +NM+   + +I   + +D+TC+TF PDL +F M  LD D VAL +RRAYDIA  ++G+ V LNG +++V  F+ YVDLY K  +D    PL VV+E    RWEV +T S  + F QVSFVNSIATTKGG H +Y+ADQ+ +K++E  KK+ K         P QVK +MW+FV CLIENPSFDSQTKENMTL QK FGS C L+EKFI     CGIV+ +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG K+S  CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y  P+SLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH  WP+LL+HNF+E+FITPI+K    NQ  SFYSIPE  EW++       W++KYYKGLGTSTSKEAKEYFSDM RH I FRYSG  D ++I LAFS+ K +ERK+WLT++M + ++RRE  + E YLYGK T  +T+ +F+N+EL+LFSN+DNERSIPCL+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TI+ L              P GQFGTRL GGKDSASPRYI T LS L++L+F   D+ +LK   DDN  +EPEWY PIIP+VL+NG DGIGTGW+ K+PNF+ +++I+N+ RM+NG  P+ M P Y+ F GTI E+  N Y+++GE++ +D TT+EI+ELP++ WT SYKE+V+E+  +G+DK PA ITDY+EY+TD TVRF+V+M   +  EAEA G HK FKLQ+PLT ++M+LFD  G ++KY    +IL+ FF++RL+ Y+ RK +L+G+L A+++KL NQARFILEKI G ++ EN     LI  L    YDSDPVK W+++ EK + ID GEE+ Q++ E ++ PD+ YL  M +  LT EKKD L K+RD KL EL+++  K+P  +W  DL    +E+  V   +     ++      K      K +K+  ET P+   RR+ P+I
Sbjct:   29 VERIYQKKTQLEHILLRPDTYIGSVEPLSQQMWVFDEDVG-LNCRDITFVPGLYKIFDEILVNAADNKQRDNSMNCIKINIDSENNTISVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSTKFTVETACAESKYSFKQTWFDNMSRAGEAKIKPFSGDDYTCITFRPDLTKFKMQSLDRDTVALMTRRAYDIAGASKGVRVLLNGTKLSVNGFRSYVDLYLKDKVDETGTPLTVVHEVVNSRWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKRNKGGVAVK---PFQVKNHMWLFVNCLIENPSFDSQTKENMTLQQKSFGSTCLLSEKFIKQANTCGIVDSIMNWVKFKAQNQLNKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTIVGL--------------PLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDNVKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEELTSNHYLISGEVSIIDSTTIEISELPVKTWTQSYKENVLEVMLNGSDKVPALITDYREYHTDSTVRFVVKMTEERLIEAEAAGLHKVFKLQSPLTCTSMVLFDHVGSLKKYEFVQDILKDFFELRLKYYVLRKAWLVGMLGAESAKLSNQARFILEKIEGTLVIENKPKKELIRMLQEMGYDSDPVKVWKQAQEKEVEIDAGEEDEQKE-EQTSGPDYNYLLSMPMWYLTKEKKDELCKQRDAKLTELNMLEKKTPSDLWQADLAAFTEELERVEQAEKESQAKVTQV---KGKGGRTKVVKMKDETLPTPQGRRVIPRI 1202          
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453])

HSP 1 Score: 1339.32 bits (3465), Expect = 0.000e+0
Identity = 680/1197 (56.81%), Postives = 878/1197 (73.35%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD++   +  + IT+ P LYKIFDEILVNA+DNK RD+ M  I+I+IDS+ N + +WNNG+GIPV  HK  +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+  E   SFKQTW +NM+   + +I   + +D+TC+TF PDL +F M  LD D VAL +RRAYDIA  ++G+ V LNG +++V  F+ YVDLY K  +D    PL VV+E    RWEV +T S  + F QVSFVNSIATTKGG H +Y+ADQ+ +K++E  KK+ K         P QVK +MW+FV CLIENPSFDSQTKENMTL QK FGS C L+EKFI     CGIV+ +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG K+S  CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y  P+SLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH  WP+LL+HNF+E+FITPI+K    NQ  SFYSIPE  EW++       W++KYYKGLGTSTSKEAKEYFSDM RH I FRYSG  D ++I LAFS+ K +ERK+WLT++M + ++RRE  + E YLYGK T  +T+ +F+N+EL+LFSN+DNERSIPCL+DG KPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TI+ L              P GQFGTRL GGKDSASPRYI T LS L++L+F   D+ +LK   DDN  +EPEWY PIIP+VL+NG DGIGTGW+ K+PNF+ +++I+N+ RM+NG  P+ M P Y+ F GTI E+  N Y+++GE++ +D TT+EI+ELP++ WT SYKE+V+E+  +G+DK PA ITDY+EY+TD TVRF+V+M   +  EAEA G HK FKLQ+PLT ++M+LFD  G ++KY    +IL+ FF++RL+ Y+ RK +L+G+L A+++KL NQARFILEKI G ++ EN     LI  L    YDSDPVK W+++ EK + ID GEE+ Q++ E ++ PD+ YL  M +  LT EKKD L K+RD KL EL+++  K+P  +W  DL    +E+  V   +     ++      K      K +K+  ET P+   RR+ P+I
Sbjct:   29 VERIYQKKTQLEHILLRPDTYIGSVEPLSQQMWVFDEDVG-LNCRDITFVPGLYKIFDEILVNAADNKQRDNSMNCIKINIDSENNTISVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSTKFTVETACAESKYSFKQTWFDNMSRAGEAKIKPFSGDDYTCITFRPDLTKFKMQSLDRDTVALMTRRAYDIAGASKGVRVLLNGTKLSVNGFRSYVDLYLKDKVDETGTPLTVVHEVVNSRWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKRNKGGVAVK---PFQVKNHMWLFVNCLIENPSFDSQTKENMTLQQKSFGSTCLLSEKFIKQANTCGIVDSIMNWVKFKAQNQLNKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTIVGL--------------PLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDNVKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEELTSNHYLISGEVSIIDSTTIEISELPVKTWTQSYKENVLEVMLNGSDKVPALITDYREYHTDSTVRFVVKMTEERLIEAEAAGLHKVFKLQSPLTCTSMVLFDHVGSLKKYEFVQDILKDFFELRLKYYVLRKAWLVGMLGAESAKLSNQARFILEKIEGTLVIENKPKKELIRMLQEMGYDSDPVKVWKQAQEKEVEIDAGEEDEQKE-EQTSGPDYNYLLSMPMWYLTKEKKDELCKQRDAKLTELNMLEKKTPSDLWQADLAAFTEELERVEQAEKESQAKVTQV---KGKGGRTKVVKMKDETLPTPQGRRVIPRI 1202          
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453])

HSP 1 Score: 1024.62 bits (2648), Expect = 0.000e+0
Identity = 515/879 (58.59%), Postives = 661/879 (75.20%), Query Frame = 3
Query: 1089 VKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYK---GLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKS-VEKLLAIDKGEEENQEDIEDSNK---------PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3686
            VK +MWIFV CL+ENP+FDSQTKENMTL  K FGS C+L+EKFI     CGIVE +  W  FK Q+ L++ CS  K +KL+ +PKLDDAN AG KNS +CTLILTEGDSAK+LAVSGL V+GRD+YG+FPLRGK+LNVREA  KQ+ ENAEI N+IKI+GLQY +KY+ PESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LL+H F+E+FITPIVK  KN Q L FYS+PE  EW+  T     W++KYYK   GLGTSTSKEAKEYF+DM+RH I+FRYSG ED  +I LAFS+ K ++RKEWLTNWM D ++RRE G+ E +LYGK T Y+T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TI++LAQ+FVGSNNIN+L P GQFGTRL GGKD+ASPRYI T +S + +L+F   D+ +LK L DDNQ +EPEWY PIIP+VL+NG DGIGTGW+S+IPNFNP+++I+N++R+++G  P+ M PWY+NF G+I E+  N+++ +GEI+ LD  T+EI+ELP+R WT +YKESV+E    G DKT   ITDYKEY+TD TVRF+++++  K  EAEA G HK FKLQ PLT ++M+LFD  G ++KY +  +IL  F++VRL+ Y  RK +L GIL A+++KL NQARFILEKI G V+ EN     LI  L A  YDSDP++AW+++ V+     +   +E+ +D E+S           PD+ Y+  M +  LT E+KD LL++RD K++EL+ ++ KSP  +W DDL +  +E++ V  ++  +    +        K G K++ + AE  PS   RRI P I
Sbjct:    1 VKNHMWIFVNCLVENPTFDSQTKENMTLQHKKFGSTCSLSEKFIKTAMGCGIVESILNWVKFKAQSQLNQNCSAKKISKLRGIPKLDDANDAGGKNSQKCTLILTEGDSAKTLAVSGLGVVGRDHYGIFPLRGKMLNVREASYKQIMENAEINNLIKIVGLQYKKKYDDPESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLQHRFLEEFITPIVKVSKNKQELPFYSMPEFDEWKNETSNYKTWKIKYYKDMPGLGTSTSKEAKEYFADMKRHRIEFRYSGPEDDNAITLAFSRKKIEDRKEWLTNWMVDRRQRREQGLPESFLYGKQTAYLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQSLMMTIVSLAQNFVGSNNINVLQPIGQFGTRLNGGKDAASPRYIFTMISTVARLLFPPVDDNLLKFLWDDNQRVEPEWYCPIIPLVLVNGADGIGTGWASRIPNFNPREIINNIRRLLDGEEPLPMLPWYKNFKGSIDELGPNQFMFSGEISILDSETIEISELPVRTWTQTYKESVLEPMLSGTDKTTPLITDYKEYHTDTTVRFIIKISREKLLEAEAAGLHKVFKLQAPLTCNSMVLFDHLGCLKKYESVADILREFYEVRLKYYSLRKEWLNGILGAESAKLSNQARFILEKIEGTVVIENKPKKELIRMLEARGYDSDPIRAWKEAQVKSNFEEEANSQEDSDDGEESGSATSSTPSSGPDYNYILNMALWCLTKERKDELLRQRDLKIQELEDLKCKSPCDLWKDDLAVFLEELDRVEAKERKDDTADMARGKGGQKKAGSKSLAMKAEVVPSPHGRRIVPVI 879          
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007762.1 (pep scaffold:Pmarinus_7.0:GL492656:1017:4077:-1 gene:ENSPMAG00000007010.1 transcript:ENSPMAT00000007762.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 374.015 bits (959), Expect = 2.561e-118
Identity = 182/326 (55.83%), Postives = 242/326 (74.23%), Query Frame = 3
Query:   39 FKMQSSLL---DESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLAD 1007
            F +Q +LL   D++ ++R+   +E++YQKKTQLEHILLRPD+Y+GS++   + MWVFD E   M  + +TY P L+KIFDEILVNA+DNK RD  MT I++ ID + N + +WNNG+GIPV  HK  +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ETS  E  K FKQ W NNM+   D  I  ++ EDFTC+TF PDL +F+MT LD+DI+A+ +RRAYDIA   +G+ VFLNG+++ +  F+ YVD Y K  +D +  PL VV+E    RWEV +T S  + F QVSFVNSIATTKGG HV+Y+ D
Sbjct:    8 FYLQKNLLNVKDDAKSKRL--SVERIYQKKTQLEHILLRPDTYIGSVEPVTQNMWVFD-EGIGMNCRDVTYVPGLFKIFDEILVNAADNKQRDPNMTCIKVTIDPENNKISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTQFTVETSCSESKKMFKQKWMNNMSKECDSVIKPSSGEDFTCITFQPDLTKFNMTILDKDIMAIMTRRAYDIAGICKGVKVFLNGQKLPLTGFRKYVDFYLKDRVDESGMPLHVVHEVVSNRWEVCLTIS-EKGFQQVSFVNSIATTKGGRHVDYVVD 329          
BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Match: TOP2 (Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant [Source:SGD;Acc:S000005032])

HSP 1 Score: 1004.2 bits (2595), Expect = 0.000e+0
Identity = 525/1065 (49.30%), Postives = 720/1065 (67.61%), Query Frame = 3
Query:  108 YQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAK-EDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHV-----LDANEEPLK------VVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLK-HNFVEQFITPIVKAF---KNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKL 3254
            YQK +QLEHIL RPD+Y+GS++ +++L W++D+E + M+EK +T  P L+KIFDEILVNA+DNK+RD  M  I ++I ++ + + + N+G+GIP+ IH +  +YIP MIFG LLTS+N++DD+K+VTGGRNGYGAKLCNIFS+EF+LET+    G+ + Q W NNM+ C  P+I+   K   +T VTF PDL RF M  LD DI+ +  RR YDI    + I V+LNGK + ++ FK+YV+LY K +     LD  E+         ++YE    RWEVA   S + +F Q+SFVNSIATT GGTHVNY+ DQ+  KI E  KKK K   ++      Q+K NM+IF+ CLIENP+F SQTKE +T   KDFGS C +  ++I+ + K  +  ++       N+    KK   T+++++ + PKL+DAN AGTK   +CTL+LTEGDSA SLAV+GLAV+GRDYYG +PLRGK+LNVREA   Q+ +NAEI  I KI+GLQ+ +KYE     K+LRYG LMIMTDQD DGSHIKGL+INF+ S +P LL    F+ +FITPI+K          ++FY++P+  +W++       W+ KYYKGLGTS ++E +EYFS+++RHL  F      D   I LAFSK KAD+RKEWL        R+ E G     +     K I   +FIN+ELILFS  DN RSIP ++DGFKPGQRKVL+ C K+NL  ELKVAQLA  V+E +AYHHGE SL  TII LAQ+FVGSNNI LL+PNG FGTR  GGKD+A+ RYI T+L+KLT+ IFH  D+ + K + +D +++EPEWY PI+PM+L+NG +GIGTGWS+ IP FNP ++I N++ ++N     +MHPW+R +TGTI E+   +Y + G I Q+    LEITELP R WT++ KE ++ +   GNDK   +I D +E + D  ++F++ ++P + A+   +GF++ FKL +P++L NM+ FD  G I+KYN+  EIL  F+ VRLE Y KRK+++   L  +  K   Q +FI   I  ++   N     +I +L
Sbjct:   12 YQKISQLEHILKRPDTYIGSVETQEQLQWIYDEETDCMIEKNVTIVPGLFKIFDEILVNAADNKVRDPSMKRIDVNIHAEEHTIEVKNDGKGIPIEIHNKENIYIPEMIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTEFILETADLNVGQKYVQKWENNMSICHPPKITSYKKGPSYTKVTFKPDLTRFGMKELDNDILGVMRRRVYDINGSVRDINVYLNGKSLKIRNFKNYVELYLKSLEKKRQLDNGEDGAAKSDIPTILYERINNRWEVAFAVS-DISFQQISFVNSIATTMGGTHVNYITDQIVKKISEILKKKKKKSVKSF-----QIKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLEYINKIMKTDLATRM-FEIADANEENALKKSDGTRKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRKKYE---DTKSLRYGHLMIMTDQDHDGSHIKGLIINFLESSFPGLLDIQGFLLEFITPIIKVSITKPTKNTIAFYNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHLKIFHSLQGNDKDYIDLAFSKKKADDRKEWL--------RQYEPGT----VLDPTLKEIPISDFINKELILFSLADNIRSIPNVLDGFKPGQRKVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQTIIGLAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIYTELNKLTRKIFHPADDPLYKYIQEDEKTVEPEWYLPILPMILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHPWFRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELPARTWTSTIKEYLL-LGLSGNDKIKPWIKDMEEQHDD-NIKFIITLSPEEMAKTRKIGFYERFKLISPISLMNMVAFDPHGKIKKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQWEVEKYSFQVKFIKMIIEKELTVTNKPRNAIIQEL 1052          
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44813 (DNA topoisomerase 2 [Source:UniProtKB/TrEMBL;Acc:A7RUU5])

HSP 1 Score: 548.895 bits (1413), Expect = 0.000e+0
Identity = 280/484 (57.85%), Postives = 364/484 (75.21%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISK-AAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLK------VVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQL 1526
            +E++Y KKTQLEHILLRPD+Y+GS++   + M+V+DD    +V ++ITY P L+KIFDEILVNA+DNK RD  M+TI+I+I+ + N + +WN+GRGIP+ +HKEHQVY+PTMIFGQLLTS+N+NDD+K+VTGGRNGYGAKLCNIFS +F +ET+  E  K FKQ W+NNM    +P I +  + +++TC+TF PDL++F M  LD D VAL +RRAYDIAA  +G++V LNGK++ VKTFKDYV L  K      EE  +      VV+E    RWEV VT S ++ F Q SFVNSIATTKGGTHVNY+ D     + +      K +K  + +KP Q+K ++W+F+ CLIENP+FDSQTKENMTL  KDFGS+C L++KFI  V   G++E +  W  FK +A L+KKCS +K +K++ +PKLDDAN AG KNS  CTLILTEGDSAK+LA+SGL+++GRD YGVFPLRGKLLNVREA  KQ+
Sbjct:   37 VERIYTKKTQLEHILLRPDTYIGSVEPNKQNMFVYDDILEAIVNREITYVPGLFKIFDEILVNAADNKQRDPAMSTIKIEINPEKNFISVWNDGRGIPIEMHKEHQVYVPTMIFGQLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSKKFSVETACLENKKRFKQVWTNNMGKAAEPVIKEFTSNKEYTCITFQPDLEKFKMETLDRDTVALLTRRAYDIAASVRGVSVILNGKKLKVKTFKDYVQLCLKGATKDKEEDDEEEKKPTVVHEVVNNRWEVCVTVS-DKGFQQASFVNSIATTKGGTHVNYVTD---QIVDKLMTVIKKKNKGGLAIKPFQIKSHLWVFINCLIENPAFDSQTKENMTLRAKDFGSKCELSDKFIKAVQNSGVMENVMNWMRFKEKAQLNKKCSGSKHSKIRGIPKLDDANDAGGKNSRHCTLILTEGDSAKTLAISGLSIVGRDRYGVFPLRGKLLNVREASHKQV 516          
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44811 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU3])

HSP 1 Score: 349.747 bits (896), Expect = 1.438e-108
Identity = 185/370 (50.00%), Postives = 252/370 (68.11%), Query Frame = 3
Query: 2451 LSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3548
            LS ++K++F   DE  LK L +DNQ IEPEWY PIIP +L+NG +GIGTG+S+K+PN++P+ + +N+++M+NG  P +M PWY+NF GTI +V + KY V G I  +D TTLEITELPI  WT SYKESV+E   HG DK P  ITDYKEY+T+  VRF++ M   K  EAE VG HK FKL+  +  SNM+LFD  G ++KY+   EIL+ FF +R+E YI RK Y+ G+L+A++ KL +QARFILEKI G ++ EN     LI  LV   Y SDPVKAW+ S +  L +D  +++    +  +      PDF YL  M + +L+ EKKD LLK+RD+K  EL+ +R KSP+ +W +DL+I   E+  V
Sbjct:    2 LSTVSKMLFPPSDEPNLKFLNEDNQRIEPEWYCPIIPTILVNGAEGIGTGYSTKVPNYDPRAIAENIRKMMNGEEPEEMLPWYKNFIGTITKVDDTKYSVTGNIEVIDDTTLEITELPIGTWTQSYKESVLEPMLHGTDKVPPCITDYKEYHTETRVRFVITMAENKMREAEQVGLHKKFKLEATINTSNMVLFDAMGCLKKYDTTREILKEFFTLRMERYIMRKAYMEGMLTAESGKLNSQARFILEKIEGTIVIENKPKRDLITMLVQRGYPSDPVKAWKDSQDTTLGVDDSDDDTMSTVSSTASGAGGPDFNYLLNMSMWSLSQEKKDELLKQRDQKAAELEDLRKKSPKDLWNEDLDIFLAELEAV 371          
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO29547 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3B9])

HSP 1 Score: 231.876 bits (590), Expect = 4.501e-70
Identity = 109/170 (64.12%), Postives = 133/170 (78.24%), Query Frame = 3
Query: 1806 LVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASL 2315
            + EW+  T     W++KYYKGLGTST KEAKEYFSDMERH I F+Y G  D ++IV+AFSK K  ERK+WLT  +E+ K RRE G+ E+YLY K TK +++ +F+N+ELILFSNTDNERSIP L+DG KPGQRKV+F C KRN  +E+KVAQLAGS+AELSAYHHGE   
Sbjct:    1 MEEWKAGTANHKSWKVKYYKGLGTSTPKEAKEYFSDMERHKINFKYEGPHDDEAIVMAFSKKKIQERKDWLTRGLEERKWRREQGLSELYLYEKDTKRVSYCDFVNKELILFSNTDNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLAGSIAELSAYHHGEVGF 170          
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44812 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU4])

HSP 1 Score: 159.844 bits (403), Expect = 1.189e-45
Identity = 76/113 (67.26%), Postives = 87/113 (76.99%), Query Frame = 3
Query: 1530 ENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKG 1868
            +NAEI NIIKI GLQY++KY+  + LK+LRYG LMIMTDQDQDGSHIKGL+INFIHS WP LLK  FVEQFITPIVK  K  +  SFYSIPE  EW+        W++KYYKG
Sbjct:    2 DNAEINNIIKITGLQYNKKYKSEDDLKSLRYGHLMIMTDQDQDGSHIKGLVINFIHSNWPGLLKLGFVEQFITPIVKVSKGKEEHSFYSIPEYEEWKAGNANHKSWKVKYYKG 114          
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO25698 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVR1])

HSP 1 Score: 79.7221 bits (195), Expect = 5.607e-16
Identity = 57/178 (32.02%), Postives = 91/178 (51.12%), Query Frame = 3
Query: 1374 NSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPES--LKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFY---SIPELVEWQKVTPGANQWRLKYYKGLGTSTSKE 1892
            +  QC + L EGDSA   A  G     R++  + PLRGK+LNV +A   ++ EN EI N+   +G+    + E P +  L  +RY K++IM D D DGSHI  L++ F       +++  +V    TP +   K  Q   +    +  +L+  QK+  G     ++ YKGLG   +++
Sbjct:   59 DPAQCEIFLVEGDSAGGTAKQGR---DRNFQAILPLRGKILNVEKAMQHKVFENEEIKNMFTALGVSIGTE-EDPRALNLSKVRYHKVVIMCDADVDGSHIATLILTFFFRYMREMIEQGYV-YIATPPLYLVKKGQKREYAWDDNQRDLIA-QKMGGGVT---IQRYKGLGEMNAEQ 227          
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229])

HSP 1 Score: 1390.56 bits (3598), Expect = 0.000e+0
Identity = 713/1202 (59.32%), Postives = 894/1202 (74.38%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQE-----DIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEP 3680
            +E++YQKKT LEHILLRPD+YVGS++   + MWVFD++   M +++ITY P LYKIFDEILVNA+DNK RD  M+ I+I ID + N + IWNNG+GIPV  HK+ ++Y+P +IFG LLTS+N++D++K+VTGGRNGYGAKLCNIFS++F +ET+ KEY  SFKQTW NNM    +P+I     +DFTCVTF PDL +F M +LD D VAL +RRAYD+A   +G+ V LNGK++ V  F+ YVDLY K  LD    PLKVV E    RWEV +T S  + F Q+SFVNSIATTKGG H++Y+ DQ+ AKI+E  KKK K         P QVK ++W+FV  LIENP+FDSQTKENMTL  K FGS+C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+ PESL++LRYGK+MIMTDQDQDGSHIKGLLINF H  WP+LLKH F+EQFITPIVKA KN Q LSFYS+PE  EW+K T     W +KYYKGLGTSTSKEAKEYF+DMERH I FRY G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E YLYG  TK++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF CLKRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNIN+L P GQFGTR+ GGKD+ASPRYI T LS LTKL+F   D  +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N   P+ M P Y+NF G I E+ +N+Y+V+GE++ LD  T+EITELP+R WT +YKESV+E    G DKTPA I DYKEY+TD TV+F+V M+  K A+AEAVG HK FKLQ+ LT ++M+LFD  G +++Y++  +IL+ FF++RL     RK++LLG L A+A+KL NQARF+LEKI GK+  EN     LI  LV   Y+SDPV AW K+ EK  A ++GE +  +     D   S+ P+F Y+  M +  LT EK + LLK+RD K  EL  ++ KS + +W +DL +  +E++ V  Q+  E   G  +  +VK    K         ET PS F RR++P
Sbjct:   40 VERVYQKKTPLEHILLRPDTYVGSVEPVSQPMWVFDEDVG-MNQREITYVPGLYKIFDEILVNAADNKQRDKNMSAIKITIDPESNTITIWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYRHSFKQTWQNNMTKTSEPKIRFFDGDDFTCVTFQPDLAKFKMEKLDRDTVALMTRRAYDVAGSCRGVKVTLNGKKLPVNGFRSYVDLYVKEKLDETGVPLKVVNEMVNDRWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVAKIIEVVKKKNKAGVTVK---PFQVKNHIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCVLSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQTKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEK--AQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDNVEAQEREEQSAGRAIK-LVKGKVGKPKAKKMNLEETMPSPFGRRVDP 1233          
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2a (DNA topoisomerase II alpha [Source:NCBI gene;Acc:101158729])

HSP 1 Score: 1357.81 bits (3513), Expect = 0.000e+0
Identity = 712/1309 (54.39%), Postives = 934/1309 (71.35%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW---QKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ---DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSG 4004
            +E++YQKKTQLEHILLRPDSY+GS++   + MWV+D +   +  + +T+ P LYKIFDEILVNA+DNK RD  M+ I+++ID + N + +WNNG+GIPV  HK  +VY+P +IFGQLLTS+N++D++K+VTGGRNGYGAKLCNIFS++F +ET+ KE  K+FKQTW +NM    D  I     E+FTC+TF PDL +F M+ LD+D VAL +RRAYD+A  ++G+ VF NGK++ +  F+ YVD+Y K  +D    PL V++E+  +RWEV +T S  + F QVSFVNSIATTKGG H++Y+ADQ+  +++E  KKK K         P QVK +MW+FV CL+ENP+FDSQTKENMTL QK FGS C+L++KF      CGIVE +  W  FK Q  L+KKCS  K TK+K +PKLDDAN AG KNS+ CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA  KQ+ ENAEI N+IKI+GLQY + Y  PESLK+LRYGK+MIMTDQDQDGSHIKGLLINFIH  WP+LL+HNF+E+FITPI+KA      LSFYSIPE   W++       W++KYYKGLGTSTS+EAKEYFSDMERH I F+YSG ED ++I LAFSK K +ERKEWLTN+M + ++RRE  + E YLYG+ TK +++ +FIN+EL+LFSN+DNERSIPCL+DGFKPGQRKVLF C KRN  +E+KVAQLAGSVAE+SAYHHGE SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F   D+ +LK   DDNQ +EPEWY PIIP VL+NG +GIGTGW+SKIPN++ ++++ N+ RM++G  P+ M P Y+ F GTI +V  N+Y+ +GE+  +D TT+EI+ELP++ WT +YKE+V+E   +G DK PA ITDYKEY+TD TVRF+V+M   K  EAEA G HK FKLQ+PLT ++M+LFD  G ++KY +  +IL+ FF +R++ Y+ RK +L+G+L A++SKL NQARFILEKI G ++ EN     LI  L    YDSDPV+AW   QK  E  +  ++ +EE  E  + S+  D+ YL  M +  LT EKK+ L K+RD K+ EL+ ++ KSP  +W +DL    +E+  +  +   D S P +           KG K +K+  ET P+   RR+ P++      + + + E KK      K+ +  E+ V +   G   E    N E  EE  LA RL K   ++  + L  KG + T    T   + K  K +P +  + ++G
Sbjct:   26 VERIYQKKTQLEHILLRPDSYIGSVEPVTQQMWVYDVDVG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVNIDVENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDEQKKVTGGRNGYGAKLCNIFSTKFTVETACKESRKTFKQTWYDNMGRAGDTNIKAFDGEEFTCITFKPDLSKFKMSTLDKDTVALMTRRAYDVAGSSKGVKVFFNGKRLPITGFRSYVDMYVKDKVDELGGPLTVIHEAVNERWEVCLTMS-EKGFQQVSFVNSIATTKGGRHIDYVADQIVGRLIEVVKKKNKAGVAVK---PFQVKNHMWLFVNCLVENPTFDSQTKENMTLQQKSFGSTCSLSDKFFKQATSCGIVESIMNWVKFKAQTQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSIGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNVIKILGLQYKKNYSDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKASNKKNQLSFYSIPEFNAWKETQSNHKSWKIKYYKGLGTSTSQEAKEYFSDMERHRIPFKYSGPEDDEAISLAFSKKKVEERKEWLTNFMVNRRQRREHNLPEDYLYGQSTKSLSYNDFINKELVLFSNSDNERSIPCLVDGFKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSPLARLVFPPVDDNLLKYNYDDNQRVEPEWYIPIIPTVLVNGAEGIGTGWASKIPNYDIREIVSNIHRMLDGEEPLPMLPNYKGFRGTIDQVMSNQYMNSGEVAIIDSTTIEISELPVKTWTQAYKENVLEPMLNGTDKVPALITDYKEYHTDATVRFVVKMTEEKLMEAEAAGLHKVFKLQSPLTCNSMVLFDYAGSLKKYESVQDILKDFFDLRMKYYVLRKEWLVGMLGAESSKLSNQARFILEKIQGTLVIENKPKKELIRMLQEMGYDSDPVRAWKEAQKKDEDDMEAEEEQEEGAEREDQSSGADYNYLLSMPMWFLTKEKKEDLCKQRDAKMTELNTLKGKSPADLWKEDLAAFSEELERLEQKEKDDASAPAK--------RPAKGKKPMKVKQETLPTPQGRRVIPRVTSTMKAEAHKKAELKKGEGRRGKKVKS-EDVVVKMEFGEECE----NLEQIEETGLAARLGKKTKTQTKRALSSKGKQTTLQFST---VSKKPKKNPWSDEESEAG 1313          
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229])

HSP 1 Score: 1171.38 bits (3029), Expect = 0.000e+0
Identity = 612/1042 (58.73%), Postives = 761/1042 (73.03%), Query Frame = 3
Query:  585 QTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQE-----DIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIE 3689
            QTW NNM    +P+I     +DFTCVTF PDL +F M +LD D VAL +RRAYD+A   +G+ V LNGK++ V  F+ YVDLY K  LD    PLKVV E    RWEV +T S  + F Q+SFVNSIATTKGG H++Y+ DQ+ AKI+E  KKK K         P QVK ++W+FV  LIENP+FDSQTKENMTL  K FGS+C L+EKFI     CGIVE +  W  FK Q  L+KKCS  K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA  KQ+ ENAEI NIIKI+GLQY + Y+ PESL++LRYGK+MIMTDQDQDGSHIKGLLINF H  WP+LLKH F+EQFITPIVKA KN Q LSFYS+PE  EW+K T     W +KYYKGLGTSTSKEAKEYF+DMERH I FRY G ED  +I LAFSK K D+RKEWLTN+MED ++RR  G+ E YLYG  TK++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF CLKRN  +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNIN+L P GQFGTR+ GGKD+ASPRYI T LS LTKL+F   D  +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N   P+ M P Y+NF G I E+ +N+Y+V+GE++ LD  T+EITELP+R WT +YKESV+E    G DKTPA I DYKEY+TD TV+F+V M+  K A+AEAVG HK FKLQ+ LT ++M+LFD  G +++Y++  +IL+ FF++RL     RK++LLG L A+A+KL NQARF+LEKI GK+  EN     LI  LV   Y+SDPV AW K+ EK  A ++GE +  +     D   S+ P+F Y+  M +  LT EK + LLK+RD K  EL  ++ KS + +W +DL +  +E++ V  Q+  E   G  +  +VK    K         ET PS F RR++P  +
Sbjct:  123 QTWQNNMTKTSEPKIRFFDGDDFTCVTFQPDLAKFKMEKLDRDTVALMTRRAYDVAGSCRGVKVTLNGKKLPVNGFRSYVDLYVKEKLDETGVPLKVVNEMVNDRWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVAKIIEVVKKKNKAGVTVK---PFQVKNHIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCVLSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQTKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEK--AQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDNVEAQEREEQSAGRAIK-LVKGKVGKPKAKKMNLEETMPSPFGRRVDPPTQ 1157          
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229])

HSP 1 Score: 351.288 bits (900), Expect = 7.387e-109
Identity = 186/372 (50.00%), Postives = 261/372 (70.16%), Query Frame = 3
Query: 2454 SKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQE-----DIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYN 3554
            S LTKL+F   D  +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N   P+ M P Y+NF G I E+ +N+Y+V+GE++ LD  T+EITELP+R WT +YKESV+E    G DKTPA I DYKEY+TD TV+F+V M+  K A+AEAVG HK FKLQ+ LT ++M+LFD  G +++Y++  +IL+ FF++RL     RK++LLG L A+A+KL NQARF+LEKI GK+  EN     LI  LV   Y+SDPV AW K+ EK  A ++GE +  +     D   S+ P+F Y+  M +  LT EK + LLK+RD K  EL  ++ KS + +W +DL +  +E++V ++
Sbjct:    5 SPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEK--AQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDVSFS 374          
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2a (DNA topoisomerase II alpha [Source:NCBI gene;Acc:101158729])

HSP 1 Score: 249.98 bits (637), Expect = 4.184e-75
Identity = 114/196 (58.16%), Postives = 153/196 (78.06%), Query Frame = 3
Query:   96 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLK 683
            +E++YQKKTQLEHILLRPDSY+GS++   + MWV+D +   +  + +T+ P LYKIFDEILVNA+DNK RD  M+ I+++ID + N + +WNNG+GIPV  HK  +VY+P +IFGQLLTS+N++D++K+VTGGRNGYGAKLCNIFS++F +ET+ KE  K+FKQTW +NM    D  I     E+FTC+TF PDLK
Sbjct:   20 VERIYQKKTQLEHILLRPDSYIGSVEPVTQQMWVYDVDVG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVNIDVENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDEQKKVTGGRNGYGAKLCNIFSTKFTVETACKESRKTFKQTWYDNMGRAGDTNIKAFDGEEFTCITFKPDLK 214          
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037559.1 (SMESG000037559.1)

HSP 1 Score: 2530.36 bits (6557), Expect = 0.000e+0
Identity = 1398/1454 (96.15%), Postives = 1409/1454 (96.91%), Query Frame = 3
Query:   45 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTNHSDDSSEGAVRKERSKRKAAIKTY 4403
            MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA  KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAG                  K+  H    AVLK LLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAW K +  KL      +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL  I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEI ENTNHSDDSSEGAVRKERSKRKAAIKTY
Sbjct:    1 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGA-----------------KIALHLVTYAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEIVENTNHSDDSSEGAVRKERSKRKAAIKTY 1437          
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037559.1 (SMESG000037559.1)

HSP 1 Score: 2515.34 bits (6518), Expect = 0.000e+0
Identity = 1391/1454 (95.67%), Postives = 1402/1454 (96.42%), Query Frame = 3
Query:   45 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTNHSDDSSEGAVRKERSKRKAAIKTY 4403
            MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA  KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASL+                               GKDSASPRYICTQLSKLTKLIFHHDDEAVLK LLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAW K +  KL      +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL  I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEI ENTNHSDDSSEGAVRKERSKRKAAIKTY
Sbjct:    1 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLI------------------------------WGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEIVENTNHSDDSSEGAVRKERSKRKAAIKTY 1424          
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037557.1 (SMESG000037557.1)

HSP 1 Score: 2313.88 bits (5995), Expect = 0.000e+0
Identity = 1297/1406 (92.25%), Postives = 1313/1406 (93.39%), Query Frame = 3
Query:  189 MWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTNHSDDSSEGAVRKERSKRKAAIKTY 4403
            MWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDID                 ++H ++        FGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVN                 +  K   KAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA  KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK LLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAW K +  KL      +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL  I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAF                        KVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKM KKPAAPKKKEIAENTNHSDDSSEGAVRKERSKRKAA+KTY
Sbjct:    1 MWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDR--------------TSSLHTDYD-------FGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVN-----------------IGCKDFRKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAF------------------------KVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMAKKPAAPKKKEIAENTNHSDDSSEGAVRKERSKRKAAVKTY 1344          
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037557.1 (SMESG000037557.1)

HSP 1 Score: 2112.81 bits (5473), Expect = 0.000e+0
Identity = 1175/1268 (92.67%), Postives = 1187/1268 (93.61%), Query Frame = 3
Query:  603 MNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTNHSDDSSEGAVRKERSKRKAAIKTY 4403
            MNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLAD                                    +CLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA  KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK LLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAW K +  KL      +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL  I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAF                        KVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKM KKPAAPKKKEIAENTNHSDDSSEGAVRKERSKRKAA+KTY
Sbjct:    1 MNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLAD------------------------------------QCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAF------------------------KVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMAKKPAAPKKKEIAENTNHSDDSSEGAVRKERSKRKAAVKTY 1208          
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1)

HSP 1 Score: 2112.04 bits (5471), Expect = 0.000e+0
Identity = 1123/1315 (85.40%), Postives = 1182/1315 (89.89%), Query Frame = 3
Query:   63 DESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKGDKP--TKTNKTNILIEKNAKVSPGNKRKRD 3998
            D+SL+    E IEK Y KKTQLEHILLRPDSYVGSIK E+K +W+FD EKN MVEK+ITYNP LYKI+DEILVNASDNKIRD KM+ IRIDI+   NM+RIWNNG+GIPV +HK+  V++PTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFS+EF+LETS+KEYGKSFKQTW+ NM    DP IS   KEDFTCVTFYPDLKRF+M+ LD++ +ALFSRRAYDIAACT GI V+LNG+QI VK FKDYV+LYTK+ LD+NEE LKV+YE +G RWEVA+TTS+N+ F QVSFVNSIATTKGGTHVN++ DQ A K+LE AKKKIK DKQAVKLK AQVKMNMW+FV+CLIENPSFDSQTKENMTLN KDFGS C L +KFIS VAKCGIVEK+TMW +FKNQALLDKKCSKTK TKLKD+PKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQ+ TP +N+WRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQL                            +WYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKK+  ETEKKKK  E KKQ ++I   V          + DN +S + EE  LAQRL+  + +EAFK L+ K  KP  TK        +K    SP  KRKR+
Sbjct:    4 DDSLSLEENESIEKRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKRITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHKDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQL----------------------------KWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKKTETKKQNQII---VKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRN 1287          
The following BLAST results are available for this feature:
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TOP2B0.000e+058.69DNA topoisomerase II beta [Source:HGNC Symbol;Acc:... [more]
TOP2B0.000e+058.69DNA topoisomerase II beta [Source:HGNC Symbol;Acc:... [more]
TOP2A0.000e+059.50DNA topoisomerase II alpha [Source:HGNC Symbol;Acc... [more]
TOP2B0.000e+060.97DNA topoisomerase II beta [Source:HGNC Symbol;Acc:... [more]
TOP2A3.758e-4858.02DNA topoisomerase II alpha [Source:HGNC Symbol;Acc... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
top-20.000e+054.66DNA topoisomerase 2 top-2 [Source:UniProtKB/Swiss... [more]
R05D3.120.000e+039.98Putative DNA topoisomerase 2, mitochondrial [Sour... [more]
cin-40.000e+050.99DNA topoisomerase-like protein cin-4 [Source:UniP... [more]
R05D3.121.758e-18042.11Putative DNA topoisomerase 2, mitochondrial [Sour... [more]
R05D3.121.758e-18042.11Putative DNA topoisomerase 2, mitochondrial [Sour... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Top20.000e+057.72gene:FBgn0284220 transcript:FBtr0332320[more]
Top20.000e+057.72gene:FBgn0284220 transcript:FBtr0081287[more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
top2b0.000e+058.32DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GEN... [more]
top2a0.000e+057.39DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GE... [more]
top2a6.813e-12749.24DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GE... [more]
top2b1.289e-3360.42DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TOP2A0.000e+059.23DNA topoisomerase II alpha [Source:NCBI gene;Acc:1... [more]
TOP2A0.000e+059.23DNA topoisomerase II alpha [Source:NCBI gene;Acc:1... [more]
TOP2A0.000e+059.23DNA topoisomerase II alpha [Source:NCBI gene;Acc:1... [more]
TOP2A0.000e+059.23DNA topoisomerase II alpha [Source:NCBI gene;Acc:1... [more]
TOP2A0.000e+058.74DNA topoisomerase II alpha [Source:NCBI gene;Acc:1... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Top2a0.000e+060.00topoisomerase (DNA) II alpha [Source:MGI Symbol;Ac... [more]
Top2b0.000e+058.53topoisomerase (DNA) II beta [Source:MGI Symbol;Acc... [more]
Top2b4.742e-5253.85topoisomerase (DNA) II beta [Source:MGI Symbol;Acc... [more]
Top2b8.586e-1139.52topoisomerase (DNA) II beta [Source:MGI Symbol;Acc... [more]
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BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q02880|TOP2B_HUMAN0.000e+058.69DNA topoisomerase 2-beta OS=Homo sapiens OX=9606 G... [more]
sp|P41515|TOP2A_CRIGR0.000e+060.49DNA topoisomerase 2-alpha OS=Cricetulus griseus OX... [more]
sp|Q01320|TOP2A_MOUSE0.000e+060.00DNA topoisomerase 2-alpha OS=Mus musculus OX=10090... [more]
sp|Q64511|TOP2B_MOUSE0.000e+058.53DNA topoisomerase 2-beta OS=Mus musculus OX=10090 ... [more]
sp|O42131|TOP2B_CHICK0.000e+058.62DNA topoisomerase 2-beta OS=Gallus gallus OX=9031 ... [more]
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BLAST of DNA topoisomerase 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2R2MQU00.000e+060.36DNA topoisomerase 2 OS=Lingula unguis OX=7574 GN=L... [more]
A0A094ZNL10.000e+055.67DNA topoisomerase 2 OS=Schistosoma haematobium OX=... [more]
A0A3Q0KH180.000e+056.33DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183... [more]
G4VPA90.000e+056.33DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183... [more]
A0A0X3PUD60.000e+060.76DNA topoisomerase 2 OS=Schistocephalus solidus OX=... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
top2b0.000e+058.99DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GEN... [more]
top2a0.000e+056.81DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GE... [more]
top2a0.000e+056.81DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
top2a0.000e+058.59DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GE... [more]
ENSPMAT00000007762.12.561e-11855.83pep scaffold:Pmarinus_7.0:GL492656:1017:4077:-1 ge... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
TOP20.000e+049.30Topoisomerase II; relieves torsional strain in DNA... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO448130.000e+057.85DNA topoisomerase 2 [Source:UniProtKB/TrEMBL;Acc:... [more]
EDO448111.438e-10850.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO295474.501e-7064.12Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448121.189e-4567.26Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO256985.607e-1632.02Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8... [more]
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BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
top2b0.000e+059.32DNA topoisomerase II beta [Source:NCBI gene;Acc:10... [more]
top2a0.000e+054.39DNA topoisomerase II alpha [Source:NCBI gene;Acc:1... [more]
top2b0.000e+058.73DNA topoisomerase II beta [Source:NCBI gene;Acc:10... [more]
top2b7.387e-10950.00DNA topoisomerase II beta [Source:NCBI gene;Acc:10... [more]
top2a4.184e-7558.16DNA topoisomerase II alpha [Source:NCBI gene;Acc:1... [more]
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BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000037559.10.000e+096.15SMESG000037559.1[more]
SMESG000037559.10.000e+095.67SMESG000037559.1[more]
SMESG000037557.10.000e+092.25SMESG000037557.1[more]
SMESG000037557.10.000e+092.67SMESG000037557.1[more]
SMESG000037565.10.000e+085.40SMESG000037565.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020974 ID=SMED30020974|Name=DNA topoisomerase 2|organism=Schmidtea mediterranea sexual|type=transcript|length=4540bp
CGCAATAAAGAACATAATTAATTTCAACTTTTAATTGTTTTAAAATGCAG
TCTTCATTGCTAGACGAATCTTTAAATGAAAGGGTTCCCGAAGATATAGA
AAAATTATATCAGAAAAAAACTCAATTAGAACATATATTACTTCGACCAG
ATTCTTATGTCGGGTCAATAAAAAAAGAAGATAAACTAATGTGGGTTTTT
GACGACGAGAAAAATGAGATGGTTGAAAAAAAGATTACTTACAATCCAGC
TCTTTATAAGATATTCGATGAAATTCTAGTTAATGCATCTGATAATAAAA
TTAGAGATTCTAAAATGACTACTATTAGAATCGATATAGATAGTAAAATG
AATATGGTCCGTATTTGGAACAATGGTAGAGGTATACCTGTTACGATACA
TAAAGAACATCAAGTTTACATACCGACTATGATTTTTGGTCAGTTATTGA
CATCAACTAACTTCAACGACGACAAAAAACAAGTGACTGGTGGCCGAAAT
GGATACGGTGCTAAACTCTGCAATATATTCAGTTCTGAATTCGTGCTGGA
AACATCAAGCAAAGAATACGGTAAAAGTTTTAAGCAGACTTGGTCGAATA
ACATGAATGATTGTTTTGATCCCGAAATTTCTAAAGCCGCCAAAGAAGAT
TTCACCTGTGTCACTTTCTATCCAGATCTTAAACGATTCAGCATGACTCG
TTTGGATGAGGATATTGTCGCTCTGTTTAGCAGGAGAGCTTATGATATAG
CAGCGTGTACGCAAGGGATCACTGTCTTTTTAAATGGCAAGCAGATCAAC
GTAAAAACCTTTAAAGATTACGTAGATTTATACACGAAACATGTTCTCGA
TGCAAACGAAGAGCCGTTGAAAGTAGTTTATGAATCTTATGGTAAAAGAT
GGGAAGTTGCCGTAACAACAAGTGTTAATGAGACATTTTCCCAAGTTAGT
TTCGTCAACAGTATTGCAACAACAAAGGGTGGAACCCATGTTAACTATTT
GGCGGATCAGTTGGCTGCAAAGATTTTAGAAAAAGCTAAAAAGAAAATTA
AAGATGATAAACAAGCTGTCAAATTAAAGCCTGCCCAAGTCAAAATGAAT
ATGTGGATATTTGTTAGATGTCTAATAGAAAATCCCAGTTTCGATTCTCA
GACAAAGGAAAATATGACTTTAAACCAAAAAGATTTCGGCTCAGAATGTA
CTTTAAATGAAAAATTCATTTCATTAGTGGCAAAATGCGGTATTGTTGAA
AAGTTAACCATGTGGACATTGTTCAAGAATCAAGCTTTATTGGACAAGAA
ATGCAGTAAAACTAAACAAACAAAACTGAAGGATATGCCAAAATTGGATG
ATGCCAATTTCGCGGGCACTAAGAACTCGCTGCAGTGTACTTTGATCTTA
ACGGAAGGAGATTCAGCCAAATCATTGGCTGTTTCGGGTTTGGCAGTTAT
CGGCAGGGATTATTATGGTGTATTTCCACTAAGGGGAAAATTATTAAATG
TCAGAGAAGCAGGTGTAAAGCAATTAACAGAAAATGCTGAAATCACAAAC
ATCATAAAGATTATTGGCTTGCAATACAGTCAAAAGTATGAATTACCGGA
ATCATTGAAGACTTTGCGATATGGAAAACTAATGATAATGACAGATCAGG
ATCAAGACGGATCTCACATAAAGGGCCTGTTGATAAATTTCATTCATTCA
AAATGGCCCAATTTATTAAAACACAATTTCGTCGAACAATTCATCACACC
AATAGTAAAAGCATTCAAAAACAACCAAGTATTATCTTTCTATAGCATTC
CTGAGCTTGTCGAATGGCAAAAAGTCACTCCAGGTGCGAATCAATGGCGG
TTGAAGTATTACAAGGGGTTGGGTACATCCACTAGTAAGGAAGCAAAGGA
ATATTTCAGTGACATGGAACGGCATTTGATTAAGTTTCGATACAGTGGCG
AAGAGGATGGTAAAAGTATTGTTTTGGCATTTTCAAAAACTAAAGCTGAT
GAGCGTAAAGAATGGTTGACAAATTGGATGGAAGACTGTAAACGCCGCCG
AGAGCTTGGAATGGACGAGGTTTATTTGTACGGAAAGGGCACAAAATACA
TCACGTTTGAAAACTTCATTAATCAGGAGTTGATTTTATTCAGCAATACG
GATAATGAGAGAAGCATTCCATGTTTAATGGACGGGTTCAAGCCAGGTCA
AAGAAAAGTGTTATTTGCCTGCTTGAAAAGGAATTTAGTGAAGGAATTAA
AAGTTGCACAATTAGCTGGATCCGTGGCTGAACTATCCGCATATCACCAC
GGTGAAGCATCCTTGATGTCAACTATCATTAATTTGGCTCAGGATTTCGT
CGGCAGCAATAACATCAATCTATTGATGCCAAACGGCCAATTCGGTACTC
GGCTAGCTGGGGGCAAAGATAGCGCTTCACCTCGTTACATATGTACTCAA
TTGAGTAAATTGACGAAATTGATTTTCCACCACGACGACGAGGCGGTATT
GAAAAATTTGCTGGATGACAATCAATCTATCGAACCAGAATGGTATGCAC
CAATTATTCCGATGGTATTAATTAACGGGTGTGACGGTATCGGAACTGGG
TGGAGTAGTAAAATTCCTAATTTCAACCCGAAAGATGTTATAGATAATTT
AAAACGAATGATTAATGGTGGAACTCCCGTGAAAATGCATCCATGGTATC
GTAATTTCACCGGAACAATCGTTGAGGTGTGTGAAAACAAGTATGTGGTT
AATGGGGAAATAAATCAACTCGATGGAACTACTTTGGAAATAACCGAGTT
ACCCATTCGAGTTTGGACTAATTCTTACAAAGAGAGCGTAATGGAAATTT
ATCGTCATGGAAACGATAAAACTCCAGCATTTATCACCGATTACAAAGAG
TACAATACGGATAAAACAGTTCGATTCCTCGTGGAAATGAATCCAGCCAA
ATACGCAGAAGCAGAAGCGGTGGGTTTCCACAAATTTTTCAAACTGCAGA
CACCTTTGACGTTGAGCAACATGATATTGTTTGATGAATGCGGAGTCATC
AGAAAATATAATAACGGAATAGAAATATTAGAGAGCTTTTTTAAAGTGCG
TTTGGAAATTTATATCAAACGGAAAAATTATTTATTGGGCATTTTATCAG
CTCAGGCGTCCAAGTTGCAAAACCAAGCGAGATTTATTTTAGAGAAAATT
AATGGAAAAGTTATTTGTGAAAATGTGAAAATTGCCGTTTTGATTGATAA
ATTGGTGGCAGCAAAATATGATTCCGATCCTGTTAAAGCTTGGCAGAAAA
GTGTTGAAAAGTTATTGGCAATCGATAAGGGAGAAGAAGAAAACCAAGAA
GATATTGAGGATTCTAACAAACCTGATTTTGGATATTTAACCCAGATGCA
AATCTTAACTTTAACAGCAGAAAAGAAAGACGCATTGTTGAAGGAGAGAG
ACGAAAAATTAAAAGAATTGGATATTATACGAAATAAATCACCGCAACAA
ATGTGGTTGGATGATTTAGAAATACTGGAAAAGGAAATAAATGTGGTTTA
TAATCAAGATACTTCGGAACCAGGGGAAATAGTGAATACCGTTGTGAAGA
AGCCTGGTAAAAAAGGTGGAAAAGCTATAAAAATAGCAGCAGAAACCGCT
CCGTCGAAATTCAGTCGCCGTATTGAACCGAAAATAGAAGAGGAATATTT
GAAAAAAATCAATTTAGAAACGGAAAAAAAGAAAAAGCTAGTTGAGAATA
AAAAGCAGAAAGAAATGATAGAAAATTTCGTTACAAGAGAATCTACAGGT
ACAAATGCAGAAGCTGGCGACAATAATTCTGAAAATGACGAAGAGCTGAC
TCTGGCTCAGAGATTGGCCAAGACTCATGGAAGTGAGGCGTTCAAAGGTT
TGATAGGAAAAGGAGATAAACCAACTAAAACAAACAAAACGAATATTTTG
ATTGAAAAAAATGCTAAAGTTAGTCCCGGTAACAAGAGAAAACGAGATAG
CGGTGAAATTATCGAATTATCCGATAATGATGACGGCGACTCCAATCAAG
CTCAAAATAAAGTTAAAACCAATAAATCAGAAATGTCCTCGGAAAGCCTA
TTTAAAAATATCAAGTTTCCGCAAAAGCAATCAAAACTTCTGGTAGAGAA
TAAAGACAAGCCCGTCGCTAAAAAAGCTAAAATTGAGAAACCCAAGACAA
TGAAGCCTAAAACCGATAAACCAAAACCCCAGAAGAAAGATGCGGCTCCT
AAAGACAAAAAAGAAGCAGCTCCGAAAATGACAAAAAAGCCAGCAGCGCC
AAAAAAGAAAGAAATCGCGGAAAATACAAATCATTCTGACGATTCATCTG
AAGGCGCTGTGAGGAAAGAAAGATCAAAGAGAAAAGCCGCCATTAAAACT
TACGATGTTTTTTCCGATGAAGAGGAATCTGTGAAGGATGACGACAGCGA
TTTCGATTTCAATGCGGAAAAGAGTAAAGCGTCAAATAAGAAGGCAAGGA
GGATTAGCAGTGACTCGGATTAAATGATTTGTATATAGAG
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protein sequence of SMED30020974-orf-1

>SMED30020974-orf-1 ID=SMED30020974-orf-1|Name=SMED30020974-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1493bp
MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMW
VFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDS
KMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGG
RNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAK
EDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQ
INVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQ
VSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVK
MNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGI
VEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTL
ILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEI
TNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFI
HSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQ
WRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTK
ADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFS
NTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAY
HHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYIC
TQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIG
TGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKY
VVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDY
KEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECG
VIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILE
KINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEEN
QEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSP
QQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAE
TAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRES
TGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTN
ILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSE
SLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDA
APKDKKEAAPKMTKKPAAPKKKEIAENTNHSDDSSEGAVRKERSKRKAAI
KTYDVFSDEEESVKDDDSDFDFNAEKSKASNKKARRISSDSD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001241Topo_IIA
IPR001154TopoII_euk
IPR002205Topo_IIA_A/C
IPR003594HATPase_C
IPR013757Topo_IIA_A_a_sf
IPR013506Topo_IIA_bsu_dom2
IPR013758Topo_IIA_A/C_ab
IPR006171TOPRIM_domain
IPR024946Arg_repress_C-like
IPR013759Topo_IIA_B_C
IPR014721Ribosomal_S5_D2-typ_fold_subgr
IPR031660TOPRIM_C
IPR018522TopoIIA_CS
IPR013760Topo_IIA-like_dom_sf
IPR020568Ribosomal_S5_D2-typ_fold
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0003918DNA topoisomerase type II (ATP-hydrolyzing) activity
GO:0005524ATP binding
GO:0000166nucleotide binding
GO:0003916DNA topoisomerase activity
GO:0016853isomerase activity
Vocabulary: biological process
TermDefinition
GO:0006265DNA topological change
GO:0006259DNA metabolic process