Calumenin-like protein

Overview
NameCalumenin-like protein
Smed IDSMED30020840
Length (bp)965
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calumenin-like protein (SMED30020840) t-SNE clustered cells

Violin plots show distribution of expression levels for Calumenin-like protein (SMED30020840) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calumenin-like protein (SMED30020840) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calumenin-like protein (SMED30020840) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020840

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30020840h1SMcG0021557 SmedASXL_019433SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30020840h1SMcG0021557 dd_Smed_v4_7226_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
prepharyngeal regionSMED30020840h1SMcG0021557 dd_Smed_v6_7226_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30020840h1SMcG0021557 OX_Smed_1.0.15336ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Calumenin-like protein vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 121.709 bits (304), Expect = 1.619e-31
Identity = 80/257 (31.13%), Postives = 140/257 (54.47%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L K++ KID ++D  +   EL+ WI+   +    ED + Q + HD + DG +S  EY  ++Y +     +    F+    + ++ +++    +R+F MAD + D +  + EF  F HPE + YM  IV+  T+ + DKN DGFI+LEEYI ++  HD   +     K++ + +    DKN+DG++D+ E   WI+P     A  EA+ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   69 FDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFN----YKQMMVRD----ERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEAL 317
[Close]
BLAST of Calumenin-like protein vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 121.324 bits (303), Expect = 1.887e-31
Identity = 80/257 (31.13%), Postives = 140/257 (54.47%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L K++ KID ++D  +   EL+ WI+   +    ED + Q + HD + DG +S  EY  ++Y +     +    F+    + ++ +++    +R+F MAD + D +  + EF  F HPE + YM  IV+  T+ + DKN DGFI+LEEYI ++  HD   +     K++ + +    DKN+DG++D+ E   WI+P     A  EA+ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFN----YKQMMVRD----ERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Human
Match: RCN2 (reticulocalbin 2 [Source:HGNC Symbol;Acc:HGNC:9935])

HSP 1 Score: 116.316 bits (290), Expect = 1.237e-29
Identity = 88/277 (31.77%), Postives = 145/277 (52.35%), Query Frame = 1 
Query:   67 NDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK----FDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-DSALNKSDE------KNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQ--FFKNRYKDEL 855
            +++ KL  +  ++ LQ +++KID + D  +   EL  WI+ + ++  ++++K Q   +D ++D  ++  EY I  Y     FDE++         +DD  +   +++ L  K++F  A+ +    L   EF+ FEHPE   YM   VI   L  +DKN DGF++LEE++ +   D   N+  E        + N  DK+ DGRLD  E+  W++P    IA EEA  L+ + + N  + L  +EIL +   ++ S AT YG QL   +F   Y DEL
Sbjct:   52 DEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENT--------ALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFLGDYRWDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLPWVVPNNQGIAQEEALHLIDEMDLNGDKKLSEEEILENPDLFLTSEATDYGRQLHDDYF---YHDEL 317
[Close]
BLAST of Calumenin-like protein vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 113.62 bits (283), Expect = 1.195e-28
Identity = 78/259 (30.12%), Postives = 139/259 (53.67%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L  ++ KID ++D  +   EL+ WI++  +    ++ ++Q +  D + DG IS  EY   +Y    D+   +   N+       ++ +++    +R+F MAD + D +  + EF  F HPE + YM  IV+  T+ + DKN DGFI+LEEYI ++  HD   +     K++ + +    DKN+DG++D+ E   WI+P     A  EA+ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYK------QMMVRD----ERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 113.62 bits (283), Expect = 1.426e-28
Identity = 78/259 (30.12%), Postives = 139/259 (53.67%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L  ++ KID ++D  +   EL+ WI++  +    ++ ++Q +  D + DG IS  EY   +Y    D+   +   N+       ++ +++    +R+F MAD + D +  + EF  F HPE + YM  IV+  T+ + DKN DGFI+LEEYI ++  HD   +     K++ + +    DKN+DG++D+ E   WI+P     A  EA+ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   69 FDQLTPEESKERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYK------QMMVRD----ERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEAL 317
[Close]
BLAST of Calumenin-like protein vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 93.9745 bits (232), Expect = 8.960e-22
Identity = 78/266 (29.32%), Postives = 133/266 (50.00%), Query Frame = 1 
Query:   70 DFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADN--DGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYI---YEIHDSALNKSDEKNYFNS--------LDKNKDGRLDRSEIWQWIMP-GIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQ 825
            +F +L  +  ++ L KL+ K+D + D  I+  EL+  I    +     D     + + A+   DG+I   +Y    Y   + + +++         +  + K I   ++++ +AD + +G LD TE+  F HPE   +M  +V+  T+++ DKN DG ++L+EYI   Y   D       E ++  S         DK+ DG+L++ E+  WIMP G D  A  EA+ L+G  + N    L++ EI+  Y  ++ S AT YG QLQ
Sbjct:   52 EFDELTPEKSKEKLAKLVPKMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKNYKAEKIVDGKIKWEDYREMVYGSADGAGQEL---------SPEYAKMIARDEKRWAVADYDSNGALDRTEYGCFMHPEDCDHMRDVVVAETVDDIDKNKDGSVDLDEYIGDMYRPDDYPELNGKEPDWVQSEREMFKEHRDKDGDGKLNQEEMRDWIMPVGFDH-AEAEARHLVGIADDNKDGKLNLDEIVAHYDTFVGSQATDYGEQLQ 307
[Close]
BLAST of Calumenin-like protein vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 93.9745 bits (232), Expect = 8.960e-22
Identity = 78/266 (29.32%), Postives = 133/266 (50.00%), Query Frame = 1 
Query:   70 DFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADN--DGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYI---YEIHDSALNKSDEKNYFNS--------LDKNKDGRLDRSEIWQWIMP-GIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQ 825
            +F +L  +  ++ L KL+ K+D + D  I+  EL+  I    +     D     + + A+   DG+I   +Y    Y   + + +++         +  + K I   ++++ +AD + +G LD TE+  F HPE   +M  +V+  T+++ DKN DG ++L+EYI   Y   D       E ++  S         DK+ DG+L++ E+  WIMP G D  A  EA+ L+G  + N    L++ EI+  Y  ++ S AT YG QLQ
Sbjct:   52 EFDELTPEKSKEKLAKLVPKMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKNYKAEKIVDGKIKWEDYREMVYGSADGAGQEL---------SPEYAKMIARDEKRWAVADYDSNGALDRTEYGCFMHPEDCDHMRDVVVAETVDDIDKNKDGSVDLDEYIGDMYRPDDYPELNGKEPDWVQSEREMFKEHRDKDGDGKLNQEEMRDWIMPVGFDH-AEAEARHLVGIADDNKDGKLNLDEIVAHYDTFVGSQATDYGEQLQ 307
[Close]
BLAST of Calumenin-like protein vs. Ensembl Celegans
Match: calu-2 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:Q9TYS8])

HSP 1 Score: 80.4925 bits (197), Expect = 3.800e-17
Identity = 65/247 (26.32%), Postives = 115/247 (46.56%), Query Frame = 1 
Query:  100 RQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHD---SALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFF 831
            ++S++K  + +D N D  +D  E+  W+  + +     ++  ++   D + DG +S  EY   S+  +E              H K     I   K  F  AD + DG L+  E  +F +PEH  +M P++I  TL   D+N DG I  +E++ E+ +   S   K + + +    DKNKDG+L   E+  W++       S EA+ LL  ++ +    L  +EI+  +  + K+ A +    L  +
Sbjct:   47 QESVEKFAKALDTNNDGFVDKSEILAWVSESYQKTVDREAVERISELDENADGFVSWEEYLADSFPDEEL-------------HNKEEESLIAQDKMYFKQADEDNDGKLNLEELASFLNPEHHPHMHPVLIAVTLLEKDQNGDGAIEEKEFLGELDEQRGSEWYKVEVERFRTVYDKNKDGKLAGDELTDWLLVDGTTAGSYEAESLLTNSDDDKDGQLSYEEIVKHHALFAKTEAAQEADHLHPY 280
[Close]
BLAST of Calumenin-like protein vs. Ensembl Celegans
Match: C56C10.9 (pep chromosome:WBcel235:II:6591048:6592351:-1 gene:WBGene00016965.1 transcript:C56C10.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C56C10.9)

HSP 1 Score: 56.6102 bits (135), Expect = 6.568e-9
Identity = 63/233 (27.04%), Postives = 107/233 (45.92%), Query Frame = 1 
Query:   97 IRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLH----DADNDGRISLSEY---FISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSA------------LNKSDEKNYFN-SLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTN 735
            + +S++++ +K D N D  +   EL+  IR NM    LE SK+   +     D + DG I   E+   F   ++ D S  E M +  T D H       +D  KR FN +D  +DG LD  E+  F HPE+       +++  +  YDKN D  ++ ++++  I  +             L K      FN  +D+N DG+    E++ ++ P    +AS+E   ++   + N
Sbjct:   63 LSESIKEMFKKTDVNDDGFLTAGELKQQIRKNMEEH-LEKSKNDSEIFFDIVDTNKDGSIVWEEFEPHFSKMHEKDHSDSELM-DVHTEDPHR------VDDEKRMFNRSDITRDGRLDRMEWHIFLHPEYSAQGLVEIVNDLMGVYDKNNDEVVSQDDFVNGIPGTVDELNPEFENMEKLEKERRLREFNEEIDENSDGKATFRELYDYVDPQNFRLASKEVNDIMMLTDAN 287
[Close]
BLAST of Calumenin-like protein vs. Ensembl Celegans
Match: cbn-1 (Calcium BiNding protein homolog [Source:UniProtKB/TrEMBL;Acc:H2KY96])

HSP 1 Score: 52.373 bits (124), Expect = 2.220e-7
Identity = 50/192 (26.04%), Postives = 82/192 (42.71%), Query Frame = 1 
Query:  109 LQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSE---YFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTT--LNNYDKNMDGFI-NLEEYIYEIHDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQW 666
            +  ++  +D+N D +++  E   + R  +R   +E+SK  +R+ DADN G ++L E        Y FDE+++                          F  AD N+DG LD  EF  F      +  A  V D    +   D N DG + N E       +  ++  +    FN  D+NK G+LD+ E+  +
Sbjct:  228 VASMVGSVDENMDGELNAPEFADFERI-IRARAIENSKKAMRVVDADNSGTLTLDEAKRVAFEHYGFDEATLAPF-----------------------FGQADENEDGQLDNVEFAGFRS----VIRARSVRDAMDKMKRIDTNGDGLVSNAEATDSTKKEDDMDSEETLALFNIADQNKSGKLDKVELADF 391
[Close]
BLAST of Calumenin-like protein vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301907)

HSP 1 Score: 122.865 bits (307), Expect = 3.569e-32
Identity = 87/270 (32.22%), Postives = 138/270 (51.11%), Query Frame = 1 
Query:   58 KVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMR-NFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI---HDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            K   +F  L  D  ++ L  L++ +D N+D  ID  EL+ WI  + +    E++  +    D D D RI+  EY   +Y  ++   +K   ++ + +D  K+  ++    K  FN AD NKDGVL   EFV F++PE    M PI+++ T+ + D + DG IN +E++ +    HD     ++++ +    D N DG L   E+  WI+P    IA++E   L    + +H   L   EILN+Y  ++ S AT YG  LQ   N   DEL
Sbjct:   78 KEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQD----KEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFVGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQNI-NHLSDEL 342
[Close]
BLAST of Calumenin-like protein vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301905)

HSP 1 Score: 122.865 bits (307), Expect = 3.569e-32
Identity = 87/270 (32.22%), Postives = 138/270 (51.11%), Query Frame = 1 
Query:   58 KVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMR-NFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI---HDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            K   +F  L  D  ++ L  L++ +D N+D  ID  EL+ WI  + +    E++  +    D D D RI+  EY   +Y  ++   +K   ++ + +D  K+  ++    K  FN AD NKDGVL   EFV F++PE    M PI+++ T+ + D + DG IN +E++ +    HD     ++++ +    D N DG L   E+  WI+P    IA++E   L    + +H   L   EILN+Y  ++ S AT YG  LQ   N   DEL
Sbjct:   78 KEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQD----KEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFVGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQNI-NHLSDEL 342
[Close]
BLAST of Calumenin-like protein vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0079033)

HSP 1 Score: 122.865 bits (307), Expect = 3.569e-32
Identity = 87/270 (32.22%), Postives = 138/270 (51.11%), Query Frame = 1 
Query:   58 KVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMR-NFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI---HDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            K   +F  L  D  ++ L  L++ +D N+D  ID  EL+ WI  + +    E++  +    D D D RI+  EY   +Y  ++   +K   ++ + +D  K+  ++    K  FN AD NKDGVL   EFV F++PE    M PI+++ T+ + D + DG IN +E++ +    HD     ++++ +    D N DG L   E+  WI+P    IA++E   L    + +H   L   EILN+Y  ++ S AT YG  LQ   N   DEL
Sbjct:   78 KEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQD----KEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFVGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQNI-NHLSDEL 342
[Close]
BLAST of Calumenin-like protein vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0079034)

HSP 1 Score: 122.865 bits (307), Expect = 3.569e-32
Identity = 87/270 (32.22%), Postives = 138/270 (51.11%), Query Frame = 1 
Query:   58 KVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMR-NFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI---HDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            K   +F  L  D  ++ L  L++ +D N+D  ID  EL+ WI  + +    E++  +    D D D RI+  EY   +Y  ++   +K   ++ + +D  K+  ++    K  FN AD NKDGVL   EFV F++PE    M PI+++ T+ + D + DG IN +E++ +    HD     ++++ +    D N DG L   E+  WI+P    IA++E   L    + +H   L   EILN+Y  ++ S AT YG  LQ   N   DEL
Sbjct:   78 KEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQD----KEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFVGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQNI-NHLSDEL 342
[Close]
BLAST of Calumenin-like protein vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301906)

HSP 1 Score: 122.865 bits (307), Expect = 3.569e-32
Identity = 87/270 (32.22%), Postives = 138/270 (51.11%), Query Frame = 1 
Query:   58 KVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMR-NFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI---HDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            K   +F  L  D  ++ L  L++ +D N+D  ID  EL+ WI  + +    E++  +    D D D RI+  EY   +Y  ++   +K   ++ + +D  K+  ++    K  FN AD NKDGVL   EFV F++PE    M PI+++ T+ + D + DG IN +E++ +    HD     ++++ +    D N DG L   E+  WI+P    IA++E   L    + +H   L   EILN+Y  ++ S AT YG  LQ   N   DEL
Sbjct:   78 KEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQD----KEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEFVGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQNI-NHLSDEL 342
[Close]
BLAST of Calumenin-like protein vs. Ensembl Zebrafish
Match: calua (calumenin a [Source:NCBI gene;Acc:415241])

HSP 1 Score: 124.02 bits (310), Expect = 1.372e-32
Identity = 87/267 (32.58%), Postives = 139/267 (52.06%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
              E+    F  L  +  +  L K+++KID + D  +   EL+ WI+   +    ++ + Q +  D +ND  IS  EY   +Y    D+   +   N+  +          +   +R+F MAD N D + D+ EF  F HPE + +M  IV+  T+ + DKN DGFI+LEEYI ++  H+  +++ +    E+  F+   DKNKDG++DR E   WI+P     A  EAK L+ +++TN    L  +EILN Y  ++ S AT +G  L
Sbjct:   55 LGEEEAKTFDDLTPEESKNRLGKIVEKIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTYLDDPEPDDGYNYKQM----------MARDERRFKMADGNGDHIADKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMYNHEDEMDEPEWVATEREQFSEFRDKNKDGKMDREETMDWILPADYDHAEAEAKHLVYESDTNKDGKLTKEEILNKYDLFVGSQATDFGEAL 311
[Close]
BLAST of Calumenin-like protein vs. Ensembl Zebrafish
Match: calua (calumenin a [Source:NCBI gene;Acc:415241])

HSP 1 Score: 124.02 bits (310), Expect = 1.372e-32
Identity = 87/267 (32.58%), Postives = 139/267 (52.06%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
              E+    F  L  +  +  L K+++KID + D  +   EL+ WI+   +    ++ + Q +  D +ND  IS  EY   +Y    D+   +   N+  +          +   +R+F MAD N D + D+ EF  F HPE + +M  IV+  T+ + DKN DGFI+LEEYI ++  H+  +++ +    E+  F+   DKNKDG++DR E   WI+P     A  EAK L+ +++TN    L  +EILN Y  ++ S AT +G  L
Sbjct:   55 LGEEEAKTFDDLTPEESKNRLGKIVEKIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTYLDDPEPDDGYNYKQM----------MARDERRFKMADGNGDHIADKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMYNHEDEMDEPEWVATEREQFSEFRDKNKDGKMDREETMDWILPADYDHAEAEAKHLVYESDTNKDGKLTKEEILNKYDLFVGSQATDFGEAL 311
[Close]
BLAST of Calumenin-like protein vs. Ensembl Zebrafish
Match: calub (calumenin b [Source:NCBI gene;Acc:394057])

HSP 1 Score: 124.02 bits (310), Expect = 1.547e-32
Identity = 86/260 (33.08%), Postives = 138/260 (53.08%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKF--DESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQ 825
            F +L  +  ++ L K+++KID++ D  +   E++ WI++  R    ED   Q + HD ++D  +S  EY  ++Y +  DE+  E   N+  +          +   +R+F MAD + D   ++ EF  F HPE F YM  IV+  T+ + DKN DG I+L EYI +++    + S+      E+  F    DKNKDGR+D+ E   WI+P     A  EAK LL +++ +    L  QEI++ Y  ++ S AT +G  LQ
Sbjct:   61 FDQLTPEESKERLGKIVEKIDEDHDGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKNATYGYILDEADPEDGFNYRQM----------MTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLNEYIGDMYSQNGDSSEPEWVKTEREQFTEFRDKNKDGRMDKDETRDWILPADYDHAEAEAKHLLYESDADKDGRLTKQEIVDKYDLFVGSQATDFGDALQ 310
[Close]
BLAST of Calumenin-like protein vs. Ensembl Zebrafish
Match: calub (calumenin b [Source:NCBI gene;Acc:394057])

HSP 1 Score: 122.094 bits (305), Expect = 7.077e-32
Identity = 85/259 (32.82%), Postives = 137/259 (52.90%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKF--DESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L K+++KID++ D  +   E++ WI++  R    ED   Q + HD ++D  +S  EY  ++Y +  DE+  E   N+  +          +   +R+F MAD + D   ++ EF  F HPE F YM  IV+  T+ + DKN DG I+L EYI +++    + S+      E+  F    DKNKDGR+D+ E   WI+P     A  EAK LL +++ +    L  QEI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGKIVEKIDEDHDGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKNATYGYILDEADPEDGFNYRQM----------MTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLNEYIGDMYSQNGDSSEPEWVKTEREQFTEFRDKNKDGRMDKDETRDWILPADYDHAEAEAKHLLYESDADKDGRLTKQEIVDKYDLFVGSQATDFGDAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 112.849 bits (281), Expect = 1.991e-28
Identity = 81/257 (31.52%), Postives = 134/257 (52.14%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  +  L K++ KID ++D  + + EL  WI++  R    E+       +D + DG+I   EY  ++Y +   +      F  VDD    +   ++  +R+F  AD + DGV    EF  F HPE F +M  IVI  T+ + DKN DG I+L+EYI ++++    +++      EK  F+   D NKDG LD +E+  WI+P     A  EA+ L+ + + ++   +  +EIL ++  ++ S AT YG  L
Sbjct:   59 FDQLSPEESKDRLGKIVDKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGYYIDT-----EFDDVDDKA-TYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEEFDFMRDIVIQETIEDIDKNGDGKIDLQEYIGDMYNPEDGETEPDWVTTEKKQFSEFRDMNKDGFLDATEVSHWILPTEVDHADNEARHLIHETDKDNDDKITKKEILENWNMFVGSQATNYGEDL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Xenopus
Match: rcn2 (reticulocalbin 2 [Source:NCBI gene;Acc:733807])

HSP 1 Score: 122.479 bits (306), Expect = 6.190e-32
Identity = 93/268 (34.70%), Postives = 147/268 (54.85%), Query Frame = 1 
Query:   88 EDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK----FDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYF-NSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            ED +++ L+ +++KID + D  +   EL  WI+ + ++  LED+K      D D DG ++  EY +  Y     +DE+++        ++D  +   ++I L  KR+F+ AD +    L+ TEF +FEHPE   +M+  VI+  L  +DK+ DGF++LEEY+ +         D      EK+ F N  DK+ DGRL+  E+  WI+P    I+ EEA  L+ + + N  Q L  +EIL +   ++ S AT YG QLQ  K+ Y DEL
Sbjct:   59 EDQLKR-LKSIIRKIDTDSDGYLTEEELSFWIQKSFKHYILEDTKEHFAEIDKDGDGIVTWDEYNMHMYDRIIDYDENTV--------LEDEEEESFRQIHLRDKRRFDHADRDDIPGLNLTEFTDFEHPEETDHMSEFVIEGALEEHDKDGDGFVSLEEYLGDYTQDPGTVEDPHWLIVEKDRFMNDYDKDGDGRLNPVELLSWIVPNNLGISQEEASHLMEEMDKNEDQRLSEEEILQNKDIFLTSEATDYGRQLQ-DKHFYHDEL 316
[Close]
BLAST of Calumenin-like protein vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 121.709 bits (304), Expect = 1.283e-31
Identity = 85/258 (32.95%), Postives = 141/258 (54.65%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-------DSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  +  L K++ ++DK+ D  I + EL  WI++  R  +LEDS+ Q + +D + DG IS  EY    Y      + K   F  V D  K   +++ +  +R+F +AD + D +    EF  F HPE + YM  IVI  T+ + DKN DG +++ EYI +++       +    K++ + + +  D NKDG++DR+EI QWI+P     A  EAK L+ +++ +    L  +EIL+++  ++ S AT YG  L
Sbjct:   62 FDQLTPEESQHRLGKIIDQMDKDNDKYITSGELFAWIKHVSRRWNLEDSEKQGKKYDTNKDGMISWDEYAKGVYGH---LLGKGEEFYDVADKDKERYRKMMMRDERRFKVADKDGDLIATREEFTAFLHPEEYGYMQDIVITETIEDIDKNDDGIVDVHEYIADMYTPNEDEPEPDWVKTERQQFTDFRDINKDGKMDRTEISQWILPHDYDHADLEAKHLVYESDKDKDGKLTKKEILDNWNMFVGSQATNYGEDL 316
[Close]
BLAST of Calumenin-like protein vs. Ensembl Xenopus
Match: calu (calumenin [Source:NCBI gene;Acc:407965])

HSP 1 Score: 113.235 bits (282), Expect = 1.925e-28
Identity = 78/257 (30.35%), Postives = 136/257 (52.92%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L +++ KID ++D  +   EL  WI    +    ED + Q + HD + D  +S  EY  ++Y +     +   +F+    + ++ +++    +R+F MAD + D +  + EF  F HPE F YM  IV+  T+ + DKN DG I+LEEYI ++  HD   N     K++ + +    DKN DG++D+ E   WI+P     A  E++ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGRIVGKIDADKDGYVTVNELRDWIIFAQKRWIYEDVERQWKGHDLNGDSMVSWEEYKNATYGYILDDPDPDNSFN----YKQMMVRD----ERRFKMADQDGDLIATKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLEEYIGDMYNHDGDANEPEWVKTEREQFVEFRDKNHDGKMDKEETKDWILPSDYDHAEAESRHLVYESDQNKDSKLTREEIVDKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 110.538 bits (275), Expect = 2.128e-27
Identity = 76/257 (29.57%), Postives = 136/257 (52.92%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-------DSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  +  L K++ KIDK++D  + + EL+ W++++      E+       +D + D  IS  EY  ++Y +    +     +   D   + + K +   +R+F +AD + D +    EF  F HPE + YM  IVI  T+ + DKN DG +++ EYI +++       +    K++ + + +  D NKDG++DR+EI QWI+P     A  EAK L+ +++ +    L  +EIL+++  ++ S AT YG  L
Sbjct:   62 FDQLTPEESQHRLGKIVDKIDKDKDGFVTDIELKEWMKHSQNRYIYENVNKHWADYDKNKDDMISWEEYKNTTYGY----LPGEEFYDVADKDKERYRKMMMRDERRFKVADKDGDLIATREEFTAFLHPEEYGYMQDIVITETIEDIDKNDDGIVDVHEYIADMYTPNEDEPEPDWVKTERQQFTDFRDINKDGKMDRTEISQWILPHDYDHADLEAKHLVYESDKDKDGKLTKKEILDNWNMFVGSQATNYGEDL 314
[Close]
BLAST of Calumenin-like protein vs. Ensembl Xenopus
Match: fgf11 (fibroblast growth factor 11 [Source:Xenbase;Acc:XB-GENE-479522])

HSP 1 Score: 110.153 bits (274), Expect = 2.455e-27
Identity = 82/268 (30.60%), Postives = 139/268 (51.87%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            F +L  +  +  L K++ +ID + D  I   EL  WI+   +    E+     + +D + D  IS  EY  ++Y +  ++ E+ ++ +      K+  ++    +R+F  AD N D   +  EF +F HPE F +M  IVI  TL + DKN DGF++ +EYI ++  H+    + D    E+  F    D NKDG++D+ EI  WI+P     A  EA+ L+ +++ +  ++L  +E+L ++  ++ S AT YG  L     R  DEL
Sbjct:   62 FDQLTPEESKDRLGKIINRIDSDSDGLITTEELTAWIKRVQKRYVYENVARVWKDYDVNKDNTISWEEYKQATYGYYLANPEEFQDAAEQFSFKKMLPRD----ERRFKQADLNGDLEANREEFTSFLHPEEFEHMKDIVILETLEDIDKNGDGFVDQDEYIADMFSHEEGQPEPDWVATEREQFADFRDINKDGKMDKEEIRHWILPQDYDHAQAEARHLIYESDLDKDKVLTKEEVLQNWNMFVGSQATNYGEDL----TRNHDEL 321
[Close]
BLAST of Calumenin-like protein vs. Ensembl Mouse
Match: Rcn2 (reticulocalbin 2 [Source:MGI Symbol;Acc:MGI:1349765])

HSP 1 Score: 121.709 bits (304), Expect = 1.027e-31
Identity = 89/277 (32.13%), Postives = 146/277 (52.71%), Query Frame = 1 
Query:   67 NDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK----FDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-DSALNKSDE------KNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQ--FFKNRYKDEL 855
            +++ KL  +  ++ LQ +++KID + D  +   EL  WI+ + ++  ++++K Q   +D ++DG ++  EY I  Y     FDE++         +DD  +   +++ L  K++F  A+ +    L   EF+ FEHPE   YM   VI   L  +DKN DGF++LEE++ +   D   N+  E        + N  DK+ DGRLD  E+  W++P    IA EEA  L+ + + N  + L  +EIL +   ++ S AT YG QL   +F   Y DEL
Sbjct:   55 DEYVKLGHEEQQRRLQSIIKKIDSDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGAVTWDEYNIQMYDRVIDFDENT--------ALDDTEEGSFRQLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFLGDYRRDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEATDYGRQLHDDYF---YHDEL 320
[Close]
BLAST of Calumenin-like protein vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 120.553 bits (301), Expect = 2.267e-31
Identity = 80/257 (31.13%), Postives = 140/257 (54.47%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L K++ KID ++D  +   EL+ WI+   +    ED + Q + HD + DG +S  EY  ++Y +     +    F+    + ++ +++    +R+F MAD + D +  + EF  F HPE + YM  IV+  T+ + DKN DGFI+LEEYI ++  HD   +     K++ + +    DKN+DG++D+ E   WI+P     A  EA+ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGKIVSKIDDDKDGFVTVDELKGWIKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFN----YKQMMVRD----ERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Mouse
Match: Rcn1 (reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559])

HSP 1 Score: 115.161 bits (287), Expect = 3.454e-29
Identity = 83/257 (32.30%), Postives = 138/257 (53.70%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  D  ++ L K++ +ID + D  +   EL++WI+   +    ++     + +D D D +IS  EY  ++Y +   +  +  + S  D HT  F K +   +R+F  +D + D      EF  F HPE F +M  IV+  TL + DKN DGF++ +EYI ++  H+    + D    E+  FN   D NKDG+LD+ EI  WI+P     A  EA+ L+ +++ N  ++L  +EIL+++  ++ S AT YG  L
Sbjct:   66 FDQLSPDESKERLGKIVDRIDSDGDGLVTTEELKLWIKRVQKRYIYDNVAKVWKDYDRDKDEKISWEEYKQATYGYYLGNPAEFHDSS--DHHT--FKKMLPRDERRFKASDLDGDLTATREEFTAFLHPEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADMFSHEDNGPEPDWVLSEREQFNDFRDLNKDGKLDKDEIRHWILPQDYDHAQAEARHLVYESDKNKDEMLTKEEILDNWNMFVGSQATNYGEDL 318
[Close]
BLAST of Calumenin-like protein vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 113.62 bits (283), Expect = 1.018e-28
Identity = 78/259 (30.12%), Postives = 139/259 (53.67%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L  ++ KID ++D  +   EL+ WI++  +    ++ ++Q +  D + DG IS  EY   +Y    D+   +   N+       ++ +++    +R+F MAD + D +  + EF  F HPE + YM  IV+  T+ + DKN DGFI+LEEYI ++  HD   +     K++ + +    DKN+DG++D+ E   WI+P     A  EA+ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYK------QMMVRD----ERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Mouse
Match: Rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:MGI Symbol;Acc:MGI:1277122])

HSP 1 Score: 111.694 bits (278), Expect = 5.391e-28
Identity = 81/274 (29.56%), Postives = 135/274 (49.27%), Query Frame = 1 
Query:   61 VNNDFSKLEEDAIRQSLQKLLQKID--KNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-------DSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            V  +F KL  +  +  L +++ ++D   + D  +   EL  WI +  +    +        +D D DGR+   E   ++Y   E   E    F  V+D  + + K +   +R+F +AD + D +    E   F HPE F +M  IV+  TL + DKN DG++ +EEYI +++       + A  +++ + +    D NKDGRLD SE+  W++P        EA  LL +++T+    L   EIL+++  ++ S AT YG  L     R+ DEL
Sbjct:   64 VAKEFDKLSPEESQARLGRIVDRMDLAGDSDGWVSLAELRAWIAHTQQRHIRDSVSAAWHTYDTDRDGRVGWEELRNATYGHYEPGEE----FHDVED-AETYKKMLARDERRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVVAETLEDLDKNKDGYVQVEEYIADLYSEEPGEEEPAWVQTERQQFREFRDLNKDGRLDGSEVGYWVLPPSQDQPLVEANHLLHESDTDKDGRLSKAEILSNWNMFVGSQATNYGEDL----TRHHDEL 328
[Close]
BLAST of Calumenin-like protein vs. UniProt/SwissProt
Match: sp|J3S9D9|RCN2V_CROAD (Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 9.148e-35
Identity = 86/259 (33.20%), Postives = 140/259 (54.05%), Query Frame = 1 
Query:   70 DFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD-------EKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            +FSKL  +  ++ L+ ++ +ID + D  +   EL  WI+++ ++  +ED+K Q + +D D DGR+S  EY I  Y      +      +T+DD  +   +++ L  K++F  A+ + D  LD  EF  FEHPE   YM   VI  +L  +DK+ DGF++L+E++ +       K D       E  + N  DK+KDG+L   E+  W+MP  + +A EEA  LL + + +  + L   EIL +   ++ S AT YG QL
Sbjct:   42 EFSKLSPEEQQKRLKVIISRIDVDLDGFLTEAELSSWIQHSFKSYIIEDAKQQFQHYDKDGDGRVSWEEYNIQMY----DRVIDFEEDTTLDDAEEESFRQLHLKDKKRFQKANKDGDSHLDFEEFAAFEHPEEADYMKEFVIQESLEEHDKDGDGFVSLQEFLGDYRRDPAAKEDPEWIVVEEDRFKNDYDKDKDGKLSPKELLTWVMPNNEGLAQEEAVHLLDEMDLDGDRRLSANEILENQDLFLNSEATDYGRQL 296
[Close]
BLAST of Calumenin-like protein vs. UniProt/SwissProt
Match: sp|Q6IQP3|CALUA_DANRE (Calumenin-A OS=Danio rerio OX=7955 GN=calua PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 8.965e-32
Identity = 87/267 (32.58%), Postives = 139/267 (52.06%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
              E+    F  L  +  +  L K+++KID + D  +   EL+ WI+   +    ++ + Q +  D +ND  IS  EY   +Y    D+   +   N+  +          +   +R+F MAD N D + D+ EF  F HPE + +M  IV+  T+ + DKN DGFI+LEEYI ++  H+  +++ +    E+  F+   DKNKDG++DR E   WI+P     A  EAK L+ +++TN    L  +EILN Y  ++ S AT +G  L
Sbjct:   53 LGEEEAKTFDDLTPEESKNRLGKIVEKIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTYLDDPEPDDGYNYKQM----------MARDERRFKMADGNGDHIADKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMYNHEDEMDEPEWVATEREQFSEFRDKNKDGKMDREETMDWILPADYDHAEAEAKHLVYESDTNKDGKLTKEEILNKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. UniProt/SwissProt
Match: sp|B5X186|CALUA_SALSA (Calumenin-A OS=Salmo salar OX=8030 GN=calua PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.751e-31
Identity = 86/267 (32.21%), Postives = 137/267 (51.31%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
              +K    F  L  +  ++ L  +++KID + D  +   EL  WI+   +    E+   Q +  D +NDG IS  EY   +Y    D+   +   N+  +          +   +R+F MAD N+D + ++ EF  F HPE + +M  IV+  T+ + DKN DGFI+L EYI ++  H+  + + D    E+  F+   DKNKDG++DR E   WI+P     A  EAK L+ ++++N    L  +EILN Y  ++ S AT +G  L
Sbjct:   53 LGQKEAKSFDDLSPEESKRRLGVIVEKIDGDSDGFVTEVELRAWIKKAQKKYIYENVDRQWKDFDVNNDGMISWEEYRNVTYGTYLDDPEPDDGYNYQHM----------MARDERRFKMADQNRDQIANKEEFTAFLHPEEYDHMKDIVVLETMEDIDKNGDGFIDLNEYIGDMYNHEDEMEEPDWVATEREQFSEFRDKNKDGKMDREETMDWILPSDYDHAEAEAKHLVYESDSNKDGKLSKEEILNKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. UniProt/SwissProt
Match: sp|Q7SXV9|CALUB_DANRE (Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 4.122e-31
Identity = 85/259 (32.82%), Postives = 137/259 (52.90%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKF--DESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L K+++KID++ D  +   E++ WI++  R    ED   Q + HD ++D  +S  EY  ++Y +  DE+  E   N+  +          +   +R+F MAD + D   ++ EF  F HPE F YM  IV+  T+ + DKN DG I+L EYI +++    + S+      E+  F    DKNKDGR+D+ E   WI+P     A  EAK LL +++ +    L  QEI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGKIVEKIDEDHDGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEYKDATYGYILDEADPEDGFNYRQM----------MTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLNEYIGDMYSQNGDSSEPEWVKTEREQFTEFRDKNKDGRMDKDETRDWILPADYDHAEAEAKHLLYESDADKDGRLTKQEIVDKYDLFVGSQATDFGDAL 309
[Close]
BLAST of Calumenin-like protein vs. UniProt/SwissProt
Match: sp|Q4U471|CALU_MESAU (Calumenin OS=Mesocricetus auratus OX=10036 GN=CALU PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 4.573e-31
Identity = 80/257 (31.13%), Postives = 140/257 (54.47%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALN-----KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L K++ KID ++D  +   EL+ WI+   +    ED + Q + HD + DG +S  EY  ++Y +     +    F+    + ++ +++    +R+F MAD + D +  + EF  F HP+ + YM  IV+  T+ + DKN DGFI+LEEYI ++  HD   +     K++ + +    DKN+DGR+D+ E   WI+P     A  EA+ L+ +++ N    L  +EI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGKIVSKIDDDKDGFVTVDELKGWIKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFN----YKQMMVRD----ERRFKMADKDGDLIATKEEFTAFPHPDEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTEREQFVEFRDKNRDGRMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. TrEMBL
Match: A0A3Q0KR76 (Putative reticulocalbin OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 3.746e-44
Identity = 103/273 (37.73%), Postives = 157/273 (57.51%), Query Frame = 1 
Query:   46 TFSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD---EKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            T S + +  F +L+ +  +  L KL  KID + + KID  EL+ WI  +  + DLE SK + + +DAD DG+++ SEY    Y +    +E  R  S  D  TK+F++ +D  K KF+ AD +K G L+ETEFV FEHP ++ +MAP  +  TL ++DK+ DGFI+  EY+    D  +NK     E+  F + D N DG+LD +E+  W+ PG D  A++E + L  + + +    L  +E+L+ +  W+ S AT YG  L+   N  KDEL
Sbjct:   68 TGSHEYSQKFKELDPEEAKSQLGKLFHKIDIDNNLKIDKQELKDWIIQSFISLDLEASKPRFKEYDADGDGQVAWSEYTNKIYGYTAQELEDFRKDSKND--TKLFIQSLDEEKLKFDSADQDKTGYLNETEFVAFEHPHNYRHMAPYELKHTLRDFDKDKDGFISELEYLA---DDKMNKDALIIERENFKNYDINGDGKLDPNEMALWVTPGFDKTATDETEHLFNETDKDKDGSLTKEEVLDQHDLWVGSQATDYGRHLE---NLPKDEL 332
[Close]
BLAST of Calumenin-like protein vs. TrEMBL
Match: A0A4Z2DY42 (Reticulocalbin-2 OS=Schistosoma japonicum OX=6182 GN=EWB00_000740 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 3.472e-43
Identity = 99/273 (36.26%), Postives = 157/273 (57.51%), Query Frame = 1 
Query:   46 TFSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD---EKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            T S + +  F +LE +  +  L KL  KID + + KID  EL+ WI  +  + DLE SK + + +DAD DG+++ SEY    Y + E  +E +R      + T +F++ ID  K +F+ AD +K G L+ETEFV FEHP ++ +MAP  +  TL ++DK+ DG+I+  EY+    D  +NK     E+  F + D N DG+LD  E+  WI PG +  A++E + L  + + +   +L  +E+++ +  W+ S AT YG  L+   N  +DEL
Sbjct:   44 TGSHEYSQKFKELEPEEAKLQLGKLFHKIDVDNNAKIDKEELKNWIIQSFISLDLEASKPRFKEYDADGDGQLAWSEYTNKIYGYTEQELEDLRKDGK--NETSLFMQSIDEEKFRFDSADQDKTGYLNETEFVAFEHPHNYRHMAPYELKHTLRDFDKDKDGYISEVEYLA---DDKMNKDALIVERENFKNYDINADGKLDHDEMALWITPGFNRTATDEMEHLFNETDKDKDGLLTKEEVIDQHDLWVGSQATDYGRHLE---NLPRDEL 308
[Close]
BLAST of Calumenin-like protein vs. TrEMBL
Match: A0A4Z2CL09 (Reticulocalbin-2 OS=Schistosoma japonicum OX=6182 GN=EWB00_010150 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 6.563e-43
Identity = 99/273 (36.26%), Postives = 157/273 (57.51%), Query Frame = 1 
Query:   46 TFSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD---EKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            T S + +  F +LE +  +  L KL  KID + + KID  EL+ WI  +  + DLE SK + + +DAD DG+++ SEY    Y + E  +E +R      + T +F++ ID  K +F+ AD +K G L+ETEFV FEHP ++ +MAP  +  TL ++DK+ DG+I+  EY+    D  +NK     E+  F + D N DG+LD  E+  WI PG +  A++E + L  + + +   +L  +E+++ +  W+ S AT YG  L+   N  +DEL
Sbjct:   67 TGSHEYSQKFKELEPEEAKLQLGKLFHKIDVDNNAKIDKEELKNWIIQSFISLDLEASKPRFKEYDADGDGQLAWSEYTNKIYGYTEQELEDLRKDGK--NETSLFMQSIDEEKFRFDSADQDKTGYLNETEFVAFEHPHNYRHMAPYELKHTLRDFDKDKDGYISEVEYLA---DDKMNKDALIVERENFKNYDINADGKLDHDEMALWITPGFNRTATDEMEHLFNETDKDKDGLLTKEEVIDQHDLWVGSQATDYGRHLE---NLPRDEL 331
[Close]
BLAST of Calumenin-like protein vs. TrEMBL
Match: A0A183QIG8 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.396e-42
Identity = 104/276 (37.68%), Postives = 157/276 (56.88%), Query Frame = 1 
Query:   46 TFSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLI---KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD---EKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            T S + +  F +L+ +  +  L KL  KID + + KID  EL+ WI  +  + DLE SK + + +DAD DG+++ SEY    Y +    +E  R  S  D  TK+F++   L+   K KF+ AD +K G L+ETEFV FEHP ++ +MAP  +  TL ++DK+ DGFI+  EY+    D  +NK     E+  F + D N DG+LD +E+  W+ PG D  A++E + L  + + +    L  +E+LN +  W+ S AT YG  L+   N  KDEL
Sbjct:   68 TGSHEYSQKFKELDPEEAKSQLGKLFHKIDIDNNLKIDKQELKDWIIQSFISLDLEASKPRFKEYDADGDGQVAWSEYTNKIYGYTAQELEDFRKDSKND--TKLFIQVKCLLYEEKLKFDSADQDKTGYLNETEFVAFEHPHNYRHMAPYELKHTLRDFDKDKDGFISELEYLA---DDKMNKDALIIERENFKNYDINGDGKLDPNEMALWVTPGFDKTATDETEHLFNETDKDKDGSLTKEEVLNQHDLWVGSQATDYGRHLE---NLPKDEL 335
[Close]
BLAST of Calumenin-like protein vs. TrEMBL
Match: G4VM03 (Putative reticulocalbin OS=Schistosoma mansoni OX=6183 GN=Smp_158150 PE=4 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 9.396e-42
Identity = 103/276 (37.32%), Postives = 157/276 (56.88%), Query Frame = 1 
Query:   46 TFSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLI---KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD---EKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            T S + +  F +L+ +  +  L KL  KID + + KID  EL+ WI  +  + DLE SK + + +DAD DG+++ SEY    Y +    +E  R  S  D  TK+F++   L+   K KF+ AD +K G L+ETEFV FEHP ++ +MAP  +  TL ++DK+ DGFI+  EY+    D  +NK     E+  F + D N DG+LD +E+  W+ PG D  A++E + L  + + +    L  +E+L+ +  W+ S AT YG  L+   N  KDEL
Sbjct:   68 TGSHEYSQKFKELDPEEAKSQLGKLFHKIDIDNNLKIDKQELKDWIIQSFISLDLEASKPRFKEYDADGDGQVAWSEYTNKIYGYTAQELEDFRKDSKND--TKLFIQVKCLLYEEKLKFDSADQDKTGYLNETEFVAFEHPHNYRHMAPYELKHTLRDFDKDKDGFISELEYLA---DDKMNKDALIIERENFKNYDINGDGKLDPNEMALWVTPGFDKTATDETEHLFNETDKDKDGSLTKEEVLDQHDLWVGSQATDYGRHLE---NLPKDEL 335
[Close]
BLAST of Calumenin-like protein vs. Ensembl Cavefish
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:103045651])

HSP 1 Score: 128.257 bits (321), Expect = 2.527e-34
Identity = 91/272 (33.46%), Postives = 147/272 (54.04%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKF---DE-SSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            F +L  +  +  L K++ +ID ++D  + + EL  WI++  R    E+     + +D D DG+I+ +EY  ++Y F   DE S +E   ++        + +++    +R+F +AD + DG+    EF  F HPE F YM  +V+  T+ + DKN DG INLEEYI +++ S   +++      EK  F+   D NKDG LD SE+ QWI+PG    A  EAK L+ + + ++   +  +EIL ++  ++ S AT YG  L     R  DEL
Sbjct:   56 FDQLTPEESKAKLGKIVDRIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWKEYDQDKDGKIAWTEYKNTTYGFYLDDEFSDVENRESYKA------MLMRD----ERRFKVADKDGDGIASREEFTAFLHPEEFSYMKDLVVQETVEDIDKNGDGKINLEEYIGDMYTSEDGENEPEWVNTEKKQFSEFRDVNKDGFLDASEVAQWILPGDVDHADIEAKHLIHETDRDNDDKMTKKEILENWNMFVGSQATNYGEDL----TRKHDEL 313
[Close]
BLAST of Calumenin-like protein vs. Ensembl Cavefish
Match: calua (calumenin [Source:NCBI gene;Acc:103043492])

HSP 1 Score: 126.716 bits (317), Expect = 1.000e-33
Identity = 87/267 (32.58%), Postives = 141/267 (52.81%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYK--FDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
              E+V   F  L  +  +  L K+++KID ++D  +   EL+ WI+   +    ++   Q +  D +NDG IS  EY   +Y    D+   +   N+  +          +   +R+F MAD N D + D+ EF  F HPE + +M  IV+  T+ + DKN DGFI+LEEYI ++  H+  +++ +    E+  F+   DKNKDG++D+ E   WI+P     A  EAK L+ ++++N    L  +EILN Y  ++ S AT +G  L
Sbjct:   53 LGEEVAKSFDDLTPEESKDRLGKIVEKIDDDQDGFVTEAELKEWIKKAQKKYIYDNVDRQWKDFDMNNDGMISWDEYKNVTYGTYLDDPEPDDGYNYKQM----------MARDERRFKMADRNGDSIADKEEFTAFLHPEEYEHMKEIVVLETMEDIDKNGDGFIDLEEYIGDMYNHEDEMDEPEWVATEREQFSEFRDKNKDGKMDKEETMDWILPADYDHAEAEAKHLVYESDSNKDGKLSKEEILNKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Cavefish
Match: calub (calumenin-B [Source:NCBI gene;Acc:103027690])

HSP 1 Score: 123.25 bits (308), Expect = 1.877e-32
Identity = 84/259 (32.43%), Postives = 139/259 (53.67%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKF--DESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L  ++ KID++ D  +   EL+ WI++  +    ED   Q + HD + DG +S  EY  ++Y +  D+S  +   N+  +          +   +R+F MAD + +   D+ EF  F HPE + YM  IV+  T+ + DKN DGFI+L+EYI +++    + ++      E+  F    DKNKDGR+D+ E   WI+P     A  EAK LL +++T+   +L  QEI++ Y  ++ S AT +G  L
Sbjct:   61 FDQLTPEESKERLGLIVDKIDEDHDAHVTVEELKKWIKHAQKRWIYEDVDRQWQSHDLNADGYVSWEEYKNATYGYILDDSDPDDGFNYRQM----------MARDERRFKMADTDGNLQADKEEFTAFLHPEEYDYMKDIVVLETMEDIDKNGDGFIDLDEYIGDMYSQDGDPTEPEWVRTEREQFTEFRDKNKDGRMDKEETRDWILPSDYDHAEAEAKHLLYESDTDKDGMLTKQEIVDKYDLFVGSQATDFGEAL 309
[Close]
BLAST of Calumenin-like protein vs. Ensembl Cavefish
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:103039743])

HSP 1 Score: 106.301 bits (264), Expect = 4.008e-26
Identity = 82/268 (30.60%), Postives = 139/268 (51.87%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            F +L     R  L K++ +ID + +  I   EL+ WI+   +    E+       +D + D +IS  EY  ++Y +  ++ E+  + +      K+  ++    +R+F +AD + D   +  EF  F HPE F +M  IV+  T+ + DKN DG ++ +EYI ++  H+    + D    E++ F+   D NKDG++D  EI  WI+P     A  EA+ LL +++T+  Q+L  +EIL ++  ++ S AT YG  L     R  DEL
Sbjct:   62 FDQLSPAESRDRLGKIVDRIDADGNGYITTEELKAWIKRVQKRYVYENVAKVWSDYDLNKDNKISWDEYKQATYGYYLANPEEFEDATDQFSFKKMLPRD----ERRFKLADLDGDLAAEREEFTAFLHPEEFDHMKEIVVLETMEDIDKNGDGHVDEDEYIADMFAHEEGGPEPDWVKTERDQFSDFRDLNKDGKMDLEEIRHWILPQDYDHAQAEARHLLLESDTDKDQMLTKEEILENWNMFVGSQATNYGEDL----TRNHDEL 321
[Close]
BLAST of Calumenin-like protein vs. Ensembl Cavefish
Match: rcn2 (reticulocalbin 2 [Source:NCBI gene;Acc:103045530])

HSP 1 Score: 96.6709 bits (239), Expect = 9.492e-23
Identity = 73/259 (28.19%), Postives = 137/259 (52.90%), Query Frame = 1 
Query:   67 NDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALN-----KSDEKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            ++  KL     R  + +++++ID + D  +   E+ +WI+   +   ++D++ +    D ++DG ++  EY +  +     ++E   N + ++D  +  L+ + L  KR+F+ AD +    L+ TEF+ F HP    +M+   I+  L+ YD + DGFI+L E+I ++  S  +     +++E   F  L D+NKDG+L+R E  +W+ P     A EEA  L+ + + +  + L   EIL +   ++ S  T YG QL
Sbjct:   40 DEIKKLSPSEQRGKMVEIVKRIDTDSDKFLTPEEITVWIQRVYKRYAMDDAQERFPGFDINSDGLVTWDEYNMVMHGH---TVEVDEN-AVLEDPEEESLRFLHLKEKRRFDFADTDGTSGLNVTEFLAFTHPSEVDHMSDFAIEDVLSEYDLDKDGFISLSEFIGDLRASESDEPSQWEAEETVRFKDLYDQNKDGKLNRDEQLRWVAPNSYGSAREEAVHLIKEMDQDGDERLSETEILKNQDIFMNSEVTDYGRQL 294
[Close]
BLAST of Calumenin-like protein vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 126.716 bits (317), Expect = 1.946e-34
Identity = 78/257 (30.35%), Postives = 136/257 (52.92%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALN-------KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L K+++KID + D  +   E+ +WI++  R    ED   Q   HD D DG +S  EY  ++Y F           S +++    + + +   +R+F  AD + DG   + EF  F HPE F +M  I++  T+ + DKN DGFI+L EYI ++++ A         K++ + + +  D NKDG++D+ E   WI+P     A  EA+ L+ +++++    L  +EIL+ +  ++ S AT +G  L
Sbjct:   63 FDQLSPEESKERLGKIVEKIDSDGDGLVTQDEMTMWIKHTQRRWVYEDVDRQWTGHDIDGDGSVSWEEYRNATYGF--------LAVSPIEEDGFNYKQMLGRDERRFKAADVDGDGQASKEEFTAFLHPEEFEHMKDIIVTETMEDIDKNGDGFIDLNEYIGDMYNQAGESEEPEWVKTERQQFTDFRDTNKDGKMDKEETMAWILPSDYDHAEAEARHLIYESDSDKDGKLSKEEILDKHDLFVGSQATNFGEAL 311
[Close]
BLAST of Calumenin-like protein vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 105.145 bits (261), Expect = 5.308e-27
Identity = 70/242 (28.93%), Postives = 121/242 (50.00%), Query Frame = 1 
Query:  118 LLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALN-------KSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            ++ +ID+N D  +   EL  WIR+  R   LE+++ Q   HD D DG ++ +EY  ++Y +         +    D +  + L++    +R+F  AD + DG     E+  F HPE   +M  +V+  T+ + DKN DGFIN++EYI +++            +S+ K +    D + DG +   E+  WI+P     A  E+K L+ + + +    L  +EI+  Y  ++ S AT +G  L
Sbjct:    2 IVTRIDENGDGLVTETELTEWIRHAQRRWVLEEAERQWSSHDLDGDGYVNWAEYKNATYSY-----LAEEDPEDPDGYRAMVLRD----ERRFQAADVDGDGRASRNEYAAFLHPEEHPHMRNLVVTETIEDIDKNGDGFINVDEYIGDMYSEESEEGDSEWVESERKQFAELRDTDGDGLMGHDEVAAWILPDDYDQAEAESKHLIHECDDDQDGNLTREEIIAKYDLFVGSQATDFGEAL 234
[Close]
BLAST of Calumenin-like protein vs. Ensembl Sea Lamprey
Match: rcn2 (reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-62])

HSP 1 Score: 87.0409 bits (214), Expect = 1.637e-20
Identity = 66/204 (32.35%), Postives = 105/204 (51.47%), Query Frame = 1 
Query:  241 DADNDGRISLSEYFISSYKF--DESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-DSALNKSD------EKNYFNS-LDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            D D DG +S  EY    Y+        E     + V    +++ +E    K+KF  AD++    L+ TEF+ FEHPE F YM   V++  L  +DK+ DGF++L E++ + H D +  +SD      E + F S  D + DG+L+ SE+  W+MP    +A +E + LL   +T+    L   E++ +   ++ S  T YG QL
Sbjct:   20 DRDGDGVVSWDEYRQHEYESLPGGDGGEAAAAAAQVAHRMQLYARE----KKKFGKADSDGVQGLNITEFLAFEHPEEFDYMQDSVLEEALLEHDKDGDGFVDLAEFLGDYHRDPSTPQSDPEWVITETDKFTSEFDIDHDGKLNPSELLSWLMPNNLEVAEDETEHLLEHMDTDGDGQLSHDEVVQNKELFLTSEVTDYGRQL 219
[Close]
BLAST of Calumenin-like protein vs. Ensembl Sea Lamprey
Match: sdf4 (stromal cell derived factor 4 [Source:NCBI gene;Acc:406360])

HSP 1 Score: 87.0409 bits (214), Expect = 3.630e-20
Identity = 69/238 (28.99%), Postives = 126/238 (52.94%), Query Frame = 1 
Query:  121 LQKIDKNRDTKIDNFELEIWI----RNNMRNADLEDSKHQLRLHDADNDGRISLSEY---FISSYKFDESSI-EKMRNFS--TVDDHTKIFLKEIDLIKRKFNMAD-ANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDE-----------KNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEIL 768
            L ++DK+RD +I   EL+ WI    R +++ A L+++K   R  D D DG ++  EY   ++ S  FDE  I E++R+     +D+ T+  +   D +K ++N AD A +D  L+E EF++F+HPEH   M   ++   +++ D++ D  ++L E+      +  N+ ++           K +   +D NKDG + RSE+ +++ P     A  EAK ++   + +    L + E+L
Sbjct:    6 LVRVDKDRDKRISAVELQEWIVEKTREHLQQA-LDENKPHFRTLDPDGDGLVTWDEYRLRYLVSKGFDEKDIAERIRDKQELKIDEETEELM---DNLKERWNQADDAPQDQKLNEKEFLSFQHPEHSRSMLSYMVQEIIHDLDQDKDQRLSLSEFASLPLGTVENQKEQDIDDDWVRERRKEFIEVIDSNKDGFITRSELEEYMDPTSSHNALSEAKQMISVADEDEDAQLSLDEVL 239
[Close]
BLAST of Calumenin-like protein vs. Ensembl Sea Lamprey
Match: rcn2 (reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-62])

HSP 1 Score: 83.9593 bits (206), Expect = 3.557e-19
Identity = 55/154 (35.71%), Postives = 87/154 (56.49%), Query Frame = 1 
Query:  385 KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-DSALNKSD------EKNYFNS-LDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            K+KF  AD++    L+ TEF+ FEHPE F YM   V++  L  +DK+ DGF++L E++ + H D +  +SD      E + F S  D + DG+L+ SE+  W+MP    +A +E + LL   +T+    L   E++ +   ++ S  T YG QL
Sbjct:   81 KKKFGKADSDGVQGLNITEFLAFEHPEEFDYMQDSVLEEALLEHDKDGDGFVDLAEFLGDYHRDPSTPQSDPEWVITETDKFTSEFDIDHDGKLNPSELLSWLMPNNLEVAEDETEHLLEHMDTDGDGQLSHDEVVQNKELFLTSEVTDYGRQL 234
[Close]
BLAST of Calumenin-like protein vs. Ensembl Nematostella
Match: EDO35844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKE6])

HSP 1 Score: 132.88 bits (333), Expect = 1.713e-36
Identity = 85/257 (33.07%), Postives = 134/257 (52.14%), Query Frame = 1 
Query:   70 DFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKS----DEKNYFN-SLDKNKDGRLDRSEIWQWIMPGID-LIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            +F  L  +  ++ L+ L++++D N+D  +   EL  W++   +   +E   + L+  DA+ DG++  +EY   +Y       E+M+ F   D             KR+F+ AD NKDG L   E   F HPE    M+ + I  T+ + D++ DG I+L+E++ E  +          DE N FN   DKNKDG+LD+ E+  WI+P  D L+A+EEAK L+   + N    L I+EI  +Y  ++ S AT YG  L
Sbjct:   60 EFDHLPPEEAKKRLRALIREVDNNKDGAVTTEELTDWVKGVFKKRSMEGVDNDLKEKDANEDGKVDWNEYSKGTYGDQTEDDEEMKEFLRRD-------------KRRFDAADTNKDGFLTREEMAIFLHPESSPEMSEVHILETIEDIDRDKDGLISLKEFLGEYEEEPGEVPDWVKDETNRFNEEYDKNKDGKLDKEEVRLWILPETDHLMAAEEAKHLVSSADDNSDGKLTIEEIEKNYAVFVGSEATDYGRAL 303
[Close]
BLAST of Calumenin-like protein vs. Ensembl Nematostella
Match: EDO42789 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0S7])

HSP 1 Score: 106.686 bits (265), Expect = 3.837e-27
Identity = 76/256 (29.69%), Postives = 130/256 (50.78%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLE-DSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYG 813
              E++  +F  L  +  R+ L+ L+ +ID N+D  ++  ELEIWIR+ M+  ++E D     R  D +ND   +  E+ + +Y F +  I K    + + D+       I+  +RK+  AD +KD  L   E+  F HP+    M P +    +   DK+ DGF++L+EY+  +     +  D++++    D+NKDG+LD  E+  W  P     A EEA+ L+   + N    L   EI+  +  +  S AT++G
Sbjct:   12 LGEEMAKEFENLSPEEARRRLRMLVPRIDINKDGFVEEAELEIWIRHKMKKWEVEEDVDAIFRDMDFNNDNHATWKEFMMRTYGFTDEDINKKWERNNLKDY-------IEDDRRKWKYADQDKDSRLTREEYEYFHHPKEHEVMIPYIAMKVMLEGDKDKDGFLSLQEYLDWLEMPDFHIMDKRDFEEKHDQNKDGKLDLKEVEDWRRPKNFNKALEEAQHLIEHADLNGDGKLSADEIVTSHEFFAGSYATQFG 260
[Close]
BLAST of Calumenin-like protein vs. Ensembl Nematostella
Match: EDO32638 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUH8])

HSP 1 Score: 102.064 bits (253), Expect = 6.644e-25
Identity = 73/276 (26.45%), Postives = 138/276 (50.00%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQ-----------LRLHDADNDGRISLS------EYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDEKNYFNS--------LDKNKDGRLDRSEIWQWIMPG-IDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L KL+ KID + D K+   EL+ WI+ + +    ED   Q           +++ D  +  R+ ++      EY  ++Y F +   +   N+  +          I   +R++  AD N+D  L + E+  F HPE + YM  +V++ TL++ DKN DG+++LEEY+ +++  +  + +E ++  +         DKN+DG++D+ E+  WI+P   D + + E   L+ + + N    L   EI++ +  +  S AT +G  L
Sbjct:   62 FDQLSPEESKERLGKLVDKIDVDHDGKVTEEELKQWIKKSAKRYVYEDVDRQWDHLKKIEHAKIKMDDLVDGKRVDMAAPIGWEEYKNNTYGFIKEDDKSEYNYDNM----------IKRDRRRWEKADINRDDKLSKEEYTAFLHPEEYEYMKDVVVEETLDDIDKNKDGYVSLEEYLGDLYPESEKEDEEPDWVKTEREQFLTVRDKNRDGKMDKDEVRDWIVPADFDHVGA-EVTHLINEADVNKDGYLTKSEIIDKHEVFAGSQATDFGDAL 326
[Close]
BLAST of Calumenin-like protein vs. Ensembl Nematostella
Match: EDO49919 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFA2])

HSP 1 Score: 95.5153 bits (236), Expect = 4.378e-23
Identity = 75/237 (31.65%), Postives = 120/237 (50.63%), Query Frame = 1 
Query:   70 DFSKLEEDAIRQSLQKLLQ-KIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD---EKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEIL 768
            DF  L  +  +Q L++L++ ++D ++D  +   EL + + N  R     +    +  HD + DG++S  E+  + +   E   +        D  TK  + E +    KF  AD N DG+LD  E+V F HP     M+  VI  TL  +D + DG I+  EY+    D A N +    EK++  S DKNKDG+LD++EI  W+ P  D+ A EE   ++ + + N    L ++EIL
Sbjct:    9 DFKGLSAEETKQKLEQLVKSEVDLDKDGFVTEDELRLRLLNTSRKHRKTEVNSTVTFHDDNKDGKVSWEEFKKAHFTHTEGKED--------DKATKEQMAEDEA---KFKYADVNGDGMLDLHEYVTFYHPGDDERMSAWVIQDTLKKHDTDKDGMISKSEYMATFSD-ANNDAKIELEKDFDTSFDKNKDGKLDQTEIRHWLFPDDDM-AKEEPAHMIKEADDNKDGKLSMEEIL 232
[Close]
BLAST of Calumenin-like protein vs. Ensembl Nematostella
Match: EDO31169 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYP9])

HSP 1 Score: 90.8929 bits (224), Expect = 2.346e-21
Identity = 78/261 (29.89%), Postives = 130/261 (49.81%), Query Frame = 1 
Query:   70 DFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEY---------IYEIHDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQ 825
            +F +L     +Q L++L  KID ++D KI   EL  WI  NM+    + S+ ++   D + DG++S  EY   + ++D  + + M N +   DH K   K  +   +++  AD + D +L   E   F HPE    M  +++   +   D + DG I+  EY         + E +  +L KS + ++ N LDK+KDG L++ E+  WI P     +S EA+ L+ + + N    L + EI+  Y  +  S AT YG  L+
Sbjct:   15 EFDELTPKQTKQRLRELFPKIDVDQDQKISLKELVEWIDVNMKKHTRKSSESRMEQMDKNKDGKVSWEEYV--NVEYDPKNEKGMSNENK--DHLKEMKKRDE---KRWKHADMDNDNLLTIDELQMFIHPEETPRMTSVLVQENMEMLDSDKDGKISFAEYAGKLSVMWRMREDNQDSL-KSLKDDFNNDLDKDKDGSLNKEELKSWIFPSGS-PSSGEAEHLMTEVDKNKDNFLTVDEIMERYELFAGSRATNYGNMLK 266
[Close]
BLAST of Calumenin-like protein vs. Ensembl Medaka
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:101175141])

HSP 1 Score: 116.701 bits (291), Expect = 1.125e-29
Identity = 88/268 (32.84%), Postives = 140/268 (52.24%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI--HDSALNKSD----EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            F +L  +  R  L K++++ID + D  I   EL+ WI+   +    E+       +D + D RIS  EY  ++Y +  ++ E+ ++    D     F K +   +R+F  AD N D   D+ EF +F HPE F +M  IV+  TL + DKN DG+++ +EYI ++  H+    + D    E+  F+   D NKDG++D+ EI  WIMP     A  EA+ L+ +++ +  Q L   EIL ++  ++ S AT YG  L     R  DEL
Sbjct:   93 FDQLTPEESRDRLGKIVERIDGDGDGYITTAELKDWIKRVQKRYVYENVAKVWTDYDLNKDNRISWDEYKQATYGYYLANPEEFQD----DKDQFSFKKMLPRDERRFKAADLNGDNTADKEEFTSFLHPEEFDHMKDIVVQETLEDIDKNGDGYVDEDEYIADMFSHEDGGPEPDWVKTEREQFSDFRDLNKDGKMDKDEIRHWIMPQDYDHAQAEARHLVYESDQDKDQKLTKDEILENWNMFVGSQATNYGEDL----TRSHDEL 352
[Close]
BLAST of Calumenin-like protein vs. Ensembl Medaka
Match: rcn3 (reticulocalbin-1 [Source:NCBI gene;Acc:101175408])

HSP 1 Score: 112.849 bits (281), Expect = 1.491e-28
Identity = 78/257 (30.35%), Postives = 134/257 (52.14%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSD------EKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  +  L K++++ID ++D  I + EL  WI++  R    ++     + +D + D +I+  EY  ++Y +         +F  V+D    +   +   +R+F  AD + DG+   +EF  F HPE F +M  +V+  TL + DKN DG I++ EYI ++      +++      EK +F+   D NKDG LD  E+  WI+PG    A  EAK L+ + +T+    +  +EIL ++  ++ S AT YG  L
Sbjct:   61 FDQLTPEESKARLAKIVERIDTDKDGFISHAELHHWIKHRQRRYIEDNVNKHWKDYDKNRDDQIAWEEYKNTTYGY-----YLGEDFDDVED-KATYKSMLARDERRFRAADRDADGIATRSEFTAFLHPEEFDHMKDVVVQETLEDIDKNKDGKIDINEYIGDMFTPEDGETEPDWVHTEKKHFSEFRDSNKDGYLDAGEVAHWILPGEVDHADNEAKHLIHETDTDKDGKVTKKEILANWNMFVGSQATNYGEDL 311
[Close]
BLAST of Calumenin-like protein vs. Ensembl Medaka
Match: calub (calumenin [Source:NCBI gene;Acc:101165303])

HSP 1 Score: 112.079 bits (279), Expect = 2.816e-28
Identity = 75/257 (29.18%), Postives = 137/257 (53.31%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIH-------DSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
            F +L  +  ++ L  L+ +ID+++D  +   E++ WI++  +    +D   Q + HD + DG +S  EY  ++Y +     +    FS    + ++  ++    +R+F MAD + D   ++ EF  F HPE + +M  IV+  T+ + DKN DGFI+L+EYI +++       +    K++ + +    DKNKDG++D+ E   WI+P     A  EAK L+ +++ +    L   EI++ Y  ++ S AT +G  L
Sbjct:   64 FDQLTPEESKERLGMLVDRIDEDKDGYVTAEEMKRWIKDAQKRWIYDDVDRQWKSHDLNGDGFVSWEEYKNATYGYVLDDQDPDDGFS----YKQMMARD----ERRFKMADQDNDMKANKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLDEYIGDMYNQDGETTEPEWVKTEREQFTEFRDKNKDGKMDKEETKDWILPSDYDHAEAEAKHLVYESDVDKDGRLTKAEIVDKYDLFVGSQATDFGEAL 312
[Close]
BLAST of Calumenin-like protein vs. Ensembl Medaka
Match: pstpip1a (proline-serine-threonine phosphatase-interacting protein 1 [Source:NCBI gene;Acc:101156493])

HSP 1 Score: 105.531 bits (262), Expect = 5.847e-26
Identity = 78/268 (29.10%), Postives = 136/268 (50.75%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISS----YKFDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKS----DEKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQL 822
              ++   +  KL  +  R+ L ++++KID N D  +   E+ +WI++  R   LED++ +    D + DG ++  EY   +    + FDES++        ++D  +  L+++ L  K++F+ AD +    L  +EF+ F HP     MA   I   L  YD + DGFI+L E+I ++     + S    +E   F  L D++KDG L+R E  +WI P     A EEA  L+ + + +  + + + E+L +   ++ S  T YG QL
Sbjct:   44 LGDETTEEIKKLTPEEQREKLLEIVKKIDTNGDNLLGAEEITLWIQHVYRKYALEDAEERFPEFDLNKDGVLTWEEYNTVAHDQLFTFDESTV--------LEDPEQDSLRQLHLKEKKRFDFADIDDTPGLSVSEFLAFTHPSEVDRMADFTIQDVLTEYDTDKDGFISLSEFIGDVRGEDNSPSKWEIEETVRFKELYDQDKDGNLNRDEQLRWIAPNSYGSAREEALHLINEMDQDGDEQISVAEVLKNQDTFMNSEVTDYGRQL 303
[Close]
BLAST of Calumenin-like protein vs. Ensembl Medaka
Match: pstpip1a (proline-serine-threonine phosphatase-interacting protein 1 [Source:NCBI gene;Acc:101156493])

HSP 1 Score: 93.2041 bits (230), Expect = 1.339e-20
Identity = 79/281 (28.11%), Postives = 133/281 (47.33%), Query Frame = 1 
Query:   49 FSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISS----YKFDESSIEKMRNFSTVDDHTKIFLKEIDLI-KRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKS----DEKNYFNSL-DKNKDGRLDRSEIWQWIMPGIDLIASEEAK---------FLLGKNNTNHVQILHIQEILN-------DYGKWIKSPATKYG 813
              ++   +  KL  +  R+ L ++++KID N D  +   E+ +WI++  R   LED++ +    D + DG ++  EY   +    + FDES++        ++D  +  L+++ L  K++F+ AD +    L  +EF+ F HP     MA   I   L  YD + DGFI+L E+I ++     + S    +E   F  L D++KDG L+R E  +WI P     A EE K          ++ + N        ++E+L         YGK + S A K G
Sbjct:   44 LGDETTEEIKKLTPEEQREKLLEIVKKIDTNGDNLLGAEEITLWIQHVYRKYALEDAEERFPEFDLNKDGVLTWEEYNTVAHDQLFTFDESTV--------LEDPEQDSLRQLHLKEKKRFDFADIDDTPGLSVSEFLAFTHPSEVDRMADFTIQDVLTEYDTDKDGFISLSEFIGDVRGEDNSPSKWEIEETVRFKELYDQDKDGNLNRDEQLRWIAPNSYGSAREEGKEFISNIGYEIIIQRLNEGRRTCKDMEELLKMRASAEEKYGKELISIARKAG 316
[Close]
BLAST of Calumenin-like protein vs. Planmine SMEST
Match: SMESG000034497.1 (SMESG000034497.1)

HSP 1 Score: 451.055 bits (1159), Expect = 4.378e-162
Identity = 220/221 (99.55%), Postives = 221/221 (100.00%), Query Frame = 1 
Query:  193 MRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 855
            MRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQW+MPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL
Sbjct:    1 MRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWMMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL 221
[Close]
BLAST of Calumenin-like protein vs. Planmine SMEST
Match: SMESG000054251.1 (SMESG000054251.1)

HSP 1 Score: 133.65 bits (335), Expect = 1.517e-36
Identity = 80/240 (33.33%), Postives = 132/240 (55.00%), Query Frame = 1 
Query:   46 TFSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEI 765
            T S   + +F  LE +  ++ L  L+ KID N D+ ++  EL  WI  + RN DLED+  +++  D +ND  ++ +EY   +Y   E  I++M    + D   K F++ + + K+KF +AD N D +LD  EF  F HP ++  M+   ++ TL ++DKN DG ++L+E++ +       +  EK  F   DK+K+G L  SEI  W+MPG +  A  E + L  + + N  + L  +EI
Sbjct:   60 TGSHHESEEFKDLEPEEAKRRLGLLVDKIDTNHDSYVNQTELFKWIMQSFRNLDLEDATTKMQDVDENNDKMLTWAEYVSKTYGHSEKEIDEMIKTGSTD--MKGFIEGVLMDKKKFQLADRNNDSMLDVPEFAKFSHPHNYPEMSTFELEKTLEDFDKNKDGGVDLKEFVVDEKMDKETEEHEKKSFAEYDKDKNGLLQGSEITDWVMPGFEKTAVSEVEHLFNETDVNKDKNLSKEEI 297
[Close]
BLAST of Calumenin-like protein vs. Planmine SMEST
Match: SMESG000076911.1 (SMESG000076911.1)

HSP 1 Score: 112.849 bits (281), Expect = 1.030e-28
Identity = 83/257 (32.30%), Postives = 135/257 (52.53%), Query Frame = 1 
Query:   73 FSKLEEDAIRQSLQKLL-QKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTK---IFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEI-----HDSALNKSDEKNYFNSLDKNKDGRLDRSEIWQWIMP-GIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWIKSPATKYG 813
            F KL  +  +  L+KL+ +K+D ++D  +   ELE+W+    + +   +            D  +   EY    Y   ++ +        +DD +K   + +K+ D   RK+ +AD N+DG LD  EF+ F+HPE +  M  IVI  T+   DKN DGF++L EYI +I      + +L K +E  ++N  DKN+D +LD +E+ +W+MP G D I + E K L+   +TN    L ++EI+  Y  ++ S  T YG
Sbjct:   59 FDKLLPEESKSRLKKLISEKVDTDKDGFVTVQELELWMITVAKLSKERERDKLFPFFSNSIDALMDYEEYSQGIYGGLQADL--------LDDVSKANLMIMKQQD--DRKWKLADENQDGKLDRNEFLMFQHPEDYPKMKSIVIQETMEVSDKNQDGFLDLNEYISDIWPNNRPEESLLKQEEDTFYNHRDKNQDKKLDETEVGEWLMPTGYDPIDA-ETKHLMHIADTNEDDQLSMEEIITHYDTFVGSQVTDYG 304
[Close]
BLAST of Calumenin-like protein vs. Planmine SMEST
Match: SMESG000034805.1 (SMESG000034805.1)

HSP 1 Score: 105.145 bits (261), Expect = 1.532e-25
Identity = 69/246 (28.05%), Postives = 132/246 (53.66%), Query Frame = 1 
Query:   76 SKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNK--SDE------KNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWI 789
            +K  ++  +  + ++ +K+DKN DTKID  EL ++I +NM+    E +  + +  D ++D ++SL EYF+  ++    ++E +   S     T+ F K+I   + +F  AD++ DG L+  E++   +P  + +MA  ++   + ++D N DG +  +EYI    D A +K  S+E      +  F+  DK+K+G++D  E +  + PG +  A EE   L  + +TN   I+ + E+ +    W+
Sbjct:   89 AKRTKEQTKTEIDEIFKKVDKNNDTKIDRDELTLYIIDNMKKLHTEITSDEFKEVDKNSDQKVSLDEYFLHKHQKTSEALENLTR-SANSSKTQDFAKKIQHERERFKAADSDSDGFLNVHEYLLMLYPVFYPHMAHTIVHEYIEDFDTNNDGLVGKDEYIKHFLDIAADKKISEEEVKKKREAEFDKYDKDKNGKIDPEEYYAILKPGYENPAKEEVDHLFKETDTNKDGIITLDEVESHAHLWL 333
[Close]
BLAST of Calumenin-like protein vs. Planmine SMEST
Match: SMESG000034805.1 (SMESG000034805.1)

HSP 1 Score: 104.375 bits (259), Expect = 2.107e-25
Identity = 69/246 (28.05%), Postives = 132/246 (53.66%), Query Frame = 1 
Query:   76 SKLEEDAIRQSLQKLLQKIDKNRDTKIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKFDESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVNFEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNK--SDE------KNYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQILHIQEILNDYGKWI 789
            +K  ++  +  + ++ +K+DKN DTKID  EL ++I +NM+    E +  + +  D ++D ++SL EYF+  ++    ++E +   S     T+ F K+I   + +F  AD++ DG L+  E++   +P  + +MA  ++   + ++D N DG +  +EYI    D A +K  S+E      +  F+  DK+K+G++D  E +  + PG +  A EE   L  + +TN   I+ + E+ +    W+
Sbjct:   79 AKRTKEQTKTEIDEIFKKVDKNNDTKIDRDELTLYIIDNMKKLHTEITSDEFKEVDKNSDQKVSLDEYFLHKHQKTSEALENLTR-SANSSKTQDFAKKIQHERERFKAADSDSDGFLNVHEYLLMLYPVFYPHMAHTIVHEYIEDFDTNNDGLVGKDEYIKHFLDIAADKKISEEEVKKKREAEFDKYDKDKNGKIDPEEYYAILKPGYENPAKEEVDHLFKETDTNKDGIITLDEVESHAHLWL 323
The following BLAST results are available for this feature:
BLAST of Calumenin-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALU1.619e-3131.13calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU1.887e-3131.13calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
RCN21.237e-2931.77reticulocalbin 2 [Source:HGNC Symbol;Acc:HGNC:9935... [more]
CALU1.195e-2830.12calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU1.426e-2830.12calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
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BLAST of Calumenin-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calu-18.960e-2229.32CALUmenin (Calcium-binding protein) homolog [Sour... [more]
calu-18.960e-2229.32CALUmenin (Calcium-binding protein) homolog [Sour... [more]
calu-23.800e-1726.32CALUmenin (Calcium-binding protein) homolog [Sour... [more]
C56C10.96.568e-927.04pep chromosome:WBcel235:II:6591048:6592351:-1 gene... [more]
cbn-12.220e-726.04Calcium BiNding protein homolog [Source:UniProtKB... [more]
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BLAST of Calumenin-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG316503.569e-3232.22gene:FBgn0031673 transcript:FBtr0301907[more]
CG316503.569e-3232.22gene:FBgn0031673 transcript:FBtr0301905[more]
CG316503.569e-3232.22gene:FBgn0031673 transcript:FBtr0079033[more]
CG316503.569e-3232.22gene:FBgn0031673 transcript:FBtr0079034[more]
CG316503.569e-3232.22gene:FBgn0031673 transcript:FBtr0301906[more]
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BLAST of Calumenin-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calua1.372e-3232.58calumenin a [Source:NCBI gene;Acc:415241][more]
calua1.372e-3232.58calumenin a [Source:NCBI gene;Acc:415241][more]
calub1.547e-3233.08calumenin b [Source:NCBI gene;Acc:394057][more]
calub7.077e-3232.82calumenin b [Source:NCBI gene;Acc:394057][more]
rcn31.991e-2831.52reticulocalbin 3, EF-hand calcium binding domain [... [more]
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BLAST of Calumenin-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rcn26.190e-3234.70reticulocalbin 2 [Source:NCBI gene;Acc:733807][more]
rcn31.283e-3132.95reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
calu1.925e-2830.35calumenin [Source:NCBI gene;Acc:407965][more]
rcn32.128e-2729.57reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
fgf112.455e-2730.60fibroblast growth factor 11 [Source:Xenbase;Acc:XB... [more]
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BLAST of Calumenin-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rcn21.027e-3132.13reticulocalbin 2 [Source:MGI Symbol;Acc:MGI:134976... [more]
Calu2.267e-3131.13calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Rcn13.454e-2932.30reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559... [more]
Calu1.018e-2830.12calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Rcn35.391e-2829.56reticulocalbin 3, EF-hand calcium binding domain [... [more]
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BLAST of Calumenin-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|J3S9D9|RCN2V_CROAD9.148e-3533.20Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE... [more]
sp|Q6IQP3|CALUA_DANRE8.965e-3232.58Calumenin-A OS=Danio rerio OX=7955 GN=calua PE=2 S... [more]
sp|B5X186|CALUA_SALSA2.751e-3132.21Calumenin-A OS=Salmo salar OX=8030 GN=calua PE=2 S... [more]
sp|Q7SXV9|CALUB_DANRE4.122e-3132.82Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 S... [more]
sp|Q4U471|CALU_MESAU4.573e-3131.13Calumenin OS=Mesocricetus auratus OX=10036 GN=CALU... [more]
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BLAST of Calumenin-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0KR763.746e-4437.73Putative reticulocalbin OS=Schistosoma mansoni OX=... [more]
A0A4Z2DY423.472e-4336.26Reticulocalbin-2 OS=Schistosoma japonicum OX=6182 ... [more]
A0A4Z2CL096.563e-4336.26Reticulocalbin-2 OS=Schistosoma japonicum OX=6182 ... [more]
A0A183QIG82.396e-4237.68Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
G4VM039.396e-4237.32Putative reticulocalbin OS=Schistosoma mansoni OX=... [more]
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BLAST of Calumenin-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rcn32.527e-3433.46reticulocalbin 3 [Source:NCBI gene;Acc:103045651][more]
calua1.000e-3332.58calumenin [Source:NCBI gene;Acc:103043492][more]
calub1.877e-3232.43calumenin-B [Source:NCBI gene;Acc:103027690][more]
rcn14.008e-2630.60reticulocalbin 1 [Source:NCBI gene;Acc:103039743][more]
rcn29.492e-2328.19reticulocalbin 2 [Source:NCBI gene;Acc:103045530][more]
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BLAST of Calumenin-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALU1.946e-3430.35calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU5.308e-2728.93calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
rcn21.637e-2032.35reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-... [more]
sdf43.630e-2028.99stromal cell derived factor 4 [Source:NCBI gene;Ac... [more]
rcn23.557e-1935.71reticulocalbin 2 [Source:ZFIN;Acc:ZDB-GENE-050706-... [more]
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BLAST of Calumenin-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calumenin-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO358441.713e-3633.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427893.837e-2729.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO326386.644e-2526.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO499194.378e-2331.65Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO311692.346e-2129.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calumenin-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rcn11.125e-2932.84reticulocalbin 1 [Source:NCBI gene;Acc:101175141][more]
rcn31.491e-2830.35reticulocalbin-1 [Source:NCBI gene;Acc:101175408][more]
calub2.816e-2829.18calumenin [Source:NCBI gene;Acc:101165303][more]
pstpip1a5.847e-2629.10proline-serine-threonine phosphatase-interacting p... [more]
pstpip1a1.339e-2028.11proline-serine-threonine phosphatase-interacting p... [more]
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BLAST of Calumenin-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000034497.14.378e-16299.55SMESG000034497.1[more]
SMESG000054251.11.517e-3633.33SMESG000054251.1[more]
SMESG000076911.11.030e-2832.30SMESG000076911.1[more]
SMESG000034805.11.532e-2528.05SMESG000034805.1[more]
SMESG000034805.12.107e-2528.05SMESG000034805.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020840 ID=SMED30020840|Name=Calumenin-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=965bp
CACGATTTTTATTTTTTATTTCTAATATTTCTAAAATTTCAATTTACATT
TTCTGAAAAAGTTAATAATGATTTTTCAAAATTGGAAGAAGATGCAATTC
GACAAAGTTTACAGAAATTATTACAAAAAATTGATAAAAATCGTGATACC
AAAATTGATAATTTCGAATTAGAAATTTGGATTAGAAATAATATGAGAAA
TGCTGATCTAGAAGATTCAAAGCATCAATTACGTTTACATGACGCCGATA
ATGACGGCCGAATTTCTTTATCGGAATACTTTATAAGTTCATACAAATTT
GATGAATCTAGCATTGAGAAAATGAGAAATTTTTCAACGGTTGATGATCA
CACTAAAATATTTTTGAAAGAGATTGATTTAATTAAAAGAAAATTTAATA
TGGCCGATGCGAATAAAGACGGTGTACTTGATGAAACAGAATTTGTTAAT
TTTGAACATCCGGAACATTTCTTGTACATGGCACCAATAGTAATAGACAC
CACATTGAATAATTATGATAAAAACATGGATGGATTTATAAATCTGGAGG
AATACATATACGAGATTCACGATTCTGCACTCAATAAATCAGATGAAAAA
AATTATTTCAATTCTTTAGATAAAAATAAAGATGGTCGATTAGACAGATC
AGAAATATGGCAGTGGATAATGCCAGGAATTGATCTGATAGCAAGTGAAG
AAGCTAAATTTTTGCTTGGTAAAAATAATACAAATCATGTTCAGATTCTG
CATATTCAAGAAATACTAAATGATTATGGAAAATGGATCAAAAGTCCGGC
GACAAAATATGGATTTCAGTTGCAATTTTTTAAAAATAGATATAAAGATG
AACTTTAAATAAATAATTTTGTTTTTAACATAATGCCATTGTGTTTTGTT
TGTTTACTAAATGTAATGTTGTTAAGACTCGGAAGTTTTCTTCACAACAA
TTTCAAGTAATATCA
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protein sequence of SMED30020840-orf-1

>SMED30020840-orf-1 ID=SMED30020840-orf-1|Name=SMED30020840-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=286bp
HDFYFLFLIFLKFQFTFSEKVNNDFSKLEEDAIRQSLQKLLQKIDKNRDT
KIDNFELEIWIRNNMRNADLEDSKHQLRLHDADNDGRISLSEYFISSYKF
DESSIEKMRNFSTVDDHTKIFLKEIDLIKRKFNMADANKDGVLDETEFVN
FEHPEHFLYMAPIVIDTTLNNYDKNMDGFINLEEYIYEIHDSALNKSDEK
NYFNSLDKNKDGRLDRSEIWQWIMPGIDLIASEEAKFLLGKNNTNHVQIL
HIQEILNDYGKWIKSPATKYGFQLQFFKNRYKDEL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 70..98
e-value: 95.0
score: 1.8
coord: 125..153
e-value: 3.8
score: 13.1
coord: 196..224
e-value: 38.0
score: 5.0
coord: 34..62
e-value: 6.2
score: 11.3
coord: 162..190
e-value: 14.0
score: 8.6
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 200..219
e-value: 1.3E-4
score: 21.3
coord: 129..148
e-value: 0.0014
score: 18.0
coord: 79..90
e-value: 0.086
score: 12.4
coord: 164..183
e-value: 0.0057
score: 16.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 30..65
score: 9.046
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 66..101
score: 7.372
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 121..156
score: 9.185
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 192..227
score: 9.994
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 158..185
score: 5.615
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 7..97
e-value: 1.2E-7
score: 33.9
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 98..256
e-value: 5.8E-14
score: 54.0
NoneNo IPR availablePANTHERPTHR10827:SF56RETICULOCALBIN-2coord: 16..283
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 16..283
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.72
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 134..146
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 205..217
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 79..91
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 122..273
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 33..149