MAP kinase-activating death domain protein

Overview
NameMAP kinase-activating death domain protein
Smed IDSMED30020839
Length (bp)5320
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of MAP kinase-activating death domain protein (SMED30020839) t-SNE clustered cells

Violin plots show distribution of expression levels for MAP kinase-activating death domain protein (SMED30020839) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MAP kinase-activating death domain protein (SMED30020839) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MAP kinase-activating death domain protein (SMED30020839) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30020839

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30020839h1SMcG0017672 Contig38308newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020839h1SMcG0017672 Contig38308uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30020839h1SMcG0017672 dd_Smed_v4_9300_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of MAP kinase-activating death domain protein vs. Ensembl Human
Match: MADD (MAP kinase activating death domain [Source:HGNC Symbol;Acc:HGNC:6766])

HSP 1 Score: 497.278 bits (1279), Expect = 3.482e-148
Identity = 269/608 (44.24%), Postives = 370/608 (60.86%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR-----------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHS--------LTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R                 +             S S + +            QS R + R  +        LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S + HD+ EIE WI+RLL SPVPV+G  R+++ +LP+ L   + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSN 598          

HSP 2 Score: 204.527 bits (519), Expect = 3.797e-52
Identity = 104/270 (38.52%), Postives = 162/270 (60.00%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTF 4516
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  IN+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+  
Sbjct: 1198 SQRVYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINL 1466          

HSP 3 Score: 58.9214 bits (141), Expect = 1.240e-7
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  869 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKD 1008          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Human
Match: MADD (MAP kinase activating death domain [Source:HGNC Symbol;Acc:HGNC:6766])

HSP 1 Score: 498.434 bits (1282), Expect = 4.527e-148
Identity = 269/608 (44.24%), Postives = 370/608 (60.86%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR-----------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHS--------LTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R                 +             S S + +            QS R + R  +        LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S + HD+ EIE WI+RLL SPVPV+G  R+++ +LP+ L   + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSN 598          

HSP 2 Score: 216.468 bits (550), Expect = 7.995e-56
Identity = 114/320 (35.62%), Postives = 186/320 (58.13%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  IN+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     +   F++  ++++C T +   F++E   P  K +    +K+  A+ I
Sbjct: 1194 SQRVYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEI 1511          

HSP 3 Score: 58.5362 bits (140), Expect = 1.326e-7
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  869 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKD 1008          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Human
Match: MADD (MAP kinase activating death domain [Source:HGNC Symbol;Acc:HGNC:6766])

HSP 1 Score: 498.434 bits (1282), Expect = 4.837e-148
Identity = 269/608 (44.24%), Postives = 370/608 (60.86%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR-----------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHS--------LTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R                 +             S S + +            QS R + R  +        LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S + HD+ EIE WI+RLL SPVPV+G  R+++ +LP+ L   + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSN 598          

HSP 2 Score: 216.468 bits (550), Expect = 7.763e-56
Identity = 114/320 (35.62%), Postives = 186/320 (58.13%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  IN+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     +   F++  ++++C T +   F++E   P  K +    +K+  A+ I
Sbjct: 1198 SQRVYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEI 1515          

HSP 3 Score: 58.5362 bits (140), Expect = 1.407e-7
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  869 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKD 1008          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Human
Match: MADD (MAP kinase activating death domain [Source:HGNC Symbol;Acc:HGNC:6766])

HSP 1 Score: 498.434 bits (1282), Expect = 6.264e-148
Identity = 269/608 (44.24%), Postives = 370/608 (60.86%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR-----------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHS--------LTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R                 +             S S + +            QS R + R  +        LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S + HD+ EIE WI+RLL SPVPV+G  R+++ +LP+ L   + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSN 598          

HSP 2 Score: 216.853 bits (551), Expect = 7.479e-56
Identity = 114/320 (35.62%), Postives = 186/320 (58.13%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  IN+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     +   F++  ++++C T +   F++E   P  K +    +K+  A+ I
Sbjct: 1218 SQRVYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEI 1535          

HSP 3 Score: 58.5362 bits (140), Expect = 1.484e-7
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  889 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKD 1028          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Human
Match: MADD (MAP kinase activating death domain [Source:HGNC Symbol;Acc:HGNC:6766])

HSP 1 Score: 497.278 bits (1279), Expect = 1.958e-147
Identity = 269/608 (44.24%), Postives = 370/608 (60.86%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR-----------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHS--------LTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R                 +             S S + +            QS R + R  +        LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S + HD+ EIE WI+RLL SPVPV+G  R+++ +LP+ L   + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSN 598          

HSP 2 Score: 196.823 bits (499), Expect = 9.148e-50
Identity = 103/290 (35.52%), Postives = 163/290 (56.21%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTF 4516
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  IN+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+  
Sbjct: 1279 SQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINL 1568          

HSP 3 Score: 58.5362 bits (140), Expect = 1.343e-7
Identity = 34/133 (25.56%), Postives = 67/133 (50.38%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  940 ENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKD 1071          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Celegans
Match: aex-3 (MAP kinase-activating death domain protein [Source:UniProtKB/Swiss-Prot;Acc:O02626])

HSP 1 Score: 437.573 bits (1124), Expect = 4.398e-128
Identity = 243/593 (40.98%), Postives = 340/593 (57.34%), Query Frame = 2
Query:  182 ICPRLIDYLSIVGLRNPNRSGHKNS---------VVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTS--RNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIK--VTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTH-SLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLP--PPADFNILIKHIKQALKVCQALNSIS----DKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            ICPRLID+L +VG RN  R   ++S           PE+LRRYP  DHK+  LP DV  FCQP+GC +T     +NA  D   FVF LT+K++ +VRYGICLNF++  +RRS  K  + +V  +                    +   KR    +H SLTSL  +SHHP    F   L +LK II+  + R  +R+  KD +W  L G   E     +  ++ EIETWI  LLSSPVPV G T++++ ++P  L +   FALPD+ RF L+DFPLH  FE++G++ AL++  A +LE K+++QS+NYN +S  IL++ ALLYPLEYMFP+  LLP +MPSAEQL  AP+PF+ G+PSSF   RK  ++PSDV   DLD   L             + +DL +P  P  D      H+K+ LK   A+N ++    D    V + D                         FG D DSVDV+ RVAMV+FFNS ++ GN+++ TR L+LYPRP+V+ Q D+F++SR   +  +  L +TQAV++F E  + PKN  F ++ +       +GDK KWF +SL P++F VY  N +
Sbjct:    8 ICPRLIDFLVVVGKRNRTRGASQSSPDATTDTTVTYPEILRRYPTDDHKDFILPTDVTVFCQPEGCTTTSARLRKNARNDPQFFVFMLTEKDSAKVRYGICLNFYQSFDRRSTPKDEIKKVPDD--------------------AHHKKR---DSHVSLTSLCFISHHPFVSIFHQVLLLLKRIIDSSNHRAAQRTGLKDVVWAILTGHYNEPIVPEVMKEIKEIETWILMLLSSPVPVPGKTKVQIEVMPMDLSQVFEFALPDHTRFTLIDFPLHIPFEILGIDMALRVLTAAMLEFKIVIQSRNYNAVSMCILSIVALLYPLEYMFPVIPLLPAYMPSAEQLLLAPTPFLIGVPSSFFHHRKIRELPSDVILVDLDTNCL------------QVPDDLYIPDLPEPDAT----HLKERLK--NAINKMTTMTVDNETSVTDAD-------------------------FGIDIDSVDVACRVAMVQFFNSANVFGNFSEHTRTLRLYPRPVVSLQTDSFLRSRPQCTQLITDLCRTQAVEYFAECCLCPKNETFVRVQAGIESAEQVGDKPKWFSESLMPVHFMVYPNNST 534          

HSP 2 Score: 194.512 bits (493), Expect = 1.487e-49
Identity = 108/315 (34.29%), Postives = 174/315 (55.24%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQ----AIQKHSEKFRSVIEPDT--SQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSA 4657
            R Y++Q+L     + +IW N+ FWE+ F D V QEREI+G+DQ+  +++DRY+ L+ +EK+RLE  EDRLL  +L+N  A+MIM       ++ K R ++GK+HI L  S  INKL+D +   +GN + L    SR    Q F V  G    G +   E+  D ++LRSI+GA   RWW ER +  T   K  ++C+ RR +++V M + + KK +EL Q    A+++ + + +  +E      +F V D  T   G L++  +G+     +   F+   N+++C T   + F++E  D     +  R + S  A
Sbjct: 1071 RHYIYQELILAV-QHQIWQNLQFWENAFVDLVAQEREIVGMDQEPSEMIDRYSALNDSEKKRLELEEDRLLSTLLHNMTAYMIMCGTGQKALQQKVRRLLGKAHIGLVCSKEINKLLDELPSTQGNFIPLKPLGSRLVQKQSFTVCPGQSSDGQMMFMEVCDDAVVLRSITGAATERWWYERLVNITYSPKTKILCLWRRHDDKVHMHKFHTKKCRELYQCMKAAMERAAARGKVNVEGRALGGEFPVHDTETNQGGLLQVRCDGVAVIFAHNQIFIGLSNIKKCNTFGGNVFLLEEFDRKKGEIIQRRYFSQMA 1384          

HSP 3 Score: 76.6406 bits (187), Expect = 1.641e-13
Identity = 50/171 (29.24%), Postives = 92/171 (53.80%), Query Frame = 2
Query: 2507 QEILKSNPTYKPISER--KPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKG 3013
            Q I K+ P  + + ER  KP      L N+++++    R  +  +   +   +   +NQ+T+++  D+ L G +  + S  ++K ++E+E+ R L+ S++N  LE  ++ ++ Y+ +V + ++  F A V I +  + GIE S+N   C GF SVF  LE+  THY    G
Sbjct:  733 QVINKTAPKAQALKERTMKP------LANRIEQSQHIVRSKTQPNPTSQQTANQQSKNQQTVKEFCDQALVGQSVGMFSAPKLKRLMEDESLRELVCSKLNLGLEVKLS-EDEYVKEVQL-TKGQFKAYVKILKACLEGIEVSFNTPGCCGFASVFHVLEIAHTHYWAMGG 895          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Celegans
Match: aex-3 (MAP kinase-activating death domain protein [Source:UniProtKB/Swiss-Prot;Acc:O02626])

HSP 1 Score: 437.573 bits (1124), Expect = 4.598e-128
Identity = 243/593 (40.98%), Postives = 340/593 (57.34%), Query Frame = 2
Query:  182 ICPRLIDYLSIVGLRNPNRSGHKNS---------VVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTS--RNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIK--VTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTH-SLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLP--PPADFNILIKHIKQALKVCQALNSIS----DKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            ICPRLID+L +VG RN  R   ++S           PE+LRRYP  DHK+  LP DV  FCQP+GC +T     +NA  D   FVF LT+K++ +VRYGICLNF++  +RRS  K  + +V  +                    +   KR    +H SLTSL  +SHHP    F   L +LK II+  + R  +R+  KD +W  L G   E     +  ++ EIETWI  LLSSPVPV G T++++ ++P  L +   FALPD+ RF L+DFPLH  FE++G++ AL++  A +LE K+++QS+NYN +S  IL++ ALLYPLEYMFP+  LLP +MPSAEQL  AP+PF+ G+PSSF   RK  ++PSDV   DLD   L             + +DL +P  P  D      H+K+ LK   A+N ++    D    V + D                         FG D DSVDV+ RVAMV+FFNS ++ GN+++ TR L+LYPRP+V+ Q D+F++SR   +  +  L +TQAV++F E  + PKN  F ++ +       +GDK KWF +SL P++F VY  N +
Sbjct:    8 ICPRLIDFLVVVGKRNRTRGASQSSPDATTDTTVTYPEILRRYPTDDHKDFILPTDVTVFCQPEGCTTTSARLRKNARNDPQFFVFMLTEKDSAKVRYGICLNFYQSFDRRSTPKDEIKKVPDD--------------------AHHKKR---DSHVSLTSLCFISHHPFVSIFHQVLLLLKRIIDSSNHRAAQRTGLKDVVWAILTGHYNEPIVPEVMKEIKEIETWILMLLSSPVPVPGKTKVQIEVMPMDLSQVFEFALPDHTRFTLIDFPLHIPFEILGIDMALRVLTAAMLEFKIVIQSRNYNAVSMCILSIVALLYPLEYMFPVIPLLPAYMPSAEQLLLAPTPFLIGVPSSFFHHRKIRELPSDVILVDLDTNCL------------QVPDDLYIPDLPEPDAT----HLKERLK--NAINKMTTMTVDNETSVTDAD-------------------------FGIDIDSVDVACRVAMVQFFNSANVFGNFSEHTRTLRLYPRPVVSLQTDSFLRSRPQCTQLITDLCRTQAVEYFAECCLCPKNETFVRVQAGIESAEQVGDKPKWFSESLMPVHFMVYPNNST 534          

HSP 2 Score: 196.052 bits (497), Expect = 6.087e-50
Identity = 109/318 (34.28%), Postives = 176/318 (55.35%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQ----AIQKHSEKFRSVIEPDT--SQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            R Y++Q+L     + +IW N+ FWE+ F D V QEREI+G+DQ+  +++DRY+ L+ +EK+RLE  EDRLL  +L+N  A+MIM       ++ K R ++GK+HI L  S  INKL+D +   +GN + L    SR    Q F V  G    G +   E+  D ++LRSI+GA   RWW ER +  T   K  ++C+ RR +++V M + + KK +EL Q    A+++ + + +  +E      +F V D  T   G L++  +G+     +   F+   N+++C T   + F++E  D     +  R + S  A+ I
Sbjct: 1071 RHYIYQELILAV-QHQIWQNLQFWENAFVDLVAQEREIVGMDQEPSEMIDRYSALNDSEKKRLELEEDRLLSTLLHNMTAYMIMCGTGQKALQQKVRRLLGKAHIGLVCSKEINKLLDELPSTQGNFIPLKPLGSRLVQKQSFTVCPGQSSDGQMMFMEVCDDAVVLRSITGAATERWWYERLVNITYSPKTKILCLWRRHDDKVHMHKFHTKKCRELYQCMKAAMERAAARGKVNVEGRALGGEFPVHDTETNQGGLLQVRCDGVAVIFAHNQIFIGLSNIKKCNTFGGNVFLLEEFDRKKGEIIQRRYFSQMADQI 1387          

HSP 3 Score: 76.6406 bits (187), Expect = 1.712e-13
Identity = 50/171 (29.24%), Postives = 92/171 (53.80%), Query Frame = 2
Query: 2507 QEILKSNPTYKPISER--KPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKG 3013
            Q I K+ P  + + ER  KP      L N+++++    R  +  +   +   +   +NQ+T+++  D+ L G +  + S  ++K ++E+E+ R L+ S++N  LE  ++ ++ Y+ +V + ++  F A V I +  + GIE S+N   C GF SVF  LE+  THY    G
Sbjct:  733 QVINKTAPKAQALKERTMKP------LANRIEQSQHIVRSKTQPNPTSQQTANQQSKNQQTVKEFCDQALVGQSVGMFSAPKLKRLMEDESLRELVCSKLNLGLEVKLS-EDEYVKEVQL-TKGQFKAYVKILKACLEGIEVSFNTPGCCGFASVFHVLEIAHTHYWAMGG 895          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Celegans
Match: lst-6 (Lateral Signaling Target [Source:UniProtKB/TrEMBL;Acc:Q18780])

HSP 1 Score: 53.9138 bits (128), Expect = 1.342e-6
Identity = 46/155 (29.68%), Postives = 78/155 (50.32%), Query Frame = 2
Query:  812 IETWIFRLLSS-PVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLD 1273
            +E++I+  L   P+P   +T +++ LL  TL    V   P     P  D  + +IF+ + VE  ++LF   LLE++++L SK+ + L T   +L AL +P  +    + +LP    S    F AP P++ G      +  + F+  S+V   DLD
Sbjct:  235 LESYIYWCLYEVPLPSPSMT-LKIPLLGTTL----VVQRPGPKELPFFDDSIGSIFDYLPVEKFIRLFSCFLLEHQILLCSKDLSRLMTVCESLLALSFPFRWQMTYAPVLPY---SKLDFFEAPVPYVMGWYYEDTVPEELFQ--SNVCVVDLD 379          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Celegans
Match: mtm-5 (Myotubularin-related protein 5 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXP3])

HSP 1 Score: 51.6026 bits (122), Expect = 7.535e-6
Identity = 27/92 (29.35%), Postives = 53/92 (57.61%), Query Frame = 2
Query:  944 PLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFL 1219
            P+    +  +F+++G +  +K+  A+L + +++L S +   LS    A+ +LLYP EY+    T+LP    S  ++  +P+PF+ G+ S F+
Sbjct:  175 PVTGKDVAMLFKMLGFQNVIKIIHALLSDCRIVLASSSLMRLSRCQNAILSLLYPFEYVHSCVTILP---DSLAEVLESPTPFLIGVLSEFV 263          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Fly
Match: Rab3-GEF (gene:FBgn0030613 transcript:FBtr0330400)

HSP 1 Score: 404.06 bits (1037), Expect = 8.605e-115
Identity = 236/578 (40.83%), Postives = 339/578 (58.65%), Query Frame = 2
Query:  557 GGRESMSRQS--KRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALN----------SISD--KSEPVKNLDEVLNNVELQP-------------------LKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISEC---------------QRLT--VDPTKIYYPPTYLQIP 2122
            GG  S S ++  KR + R  SLTSL I+SHHP F  F+ CL ILK++I+  +E    +  G      +D++W  L G   + T S++ HDV EIETWI RLLS+PVPV G TR+E+ +L  T+ +P++FALPD+ RF LVDFPLH   EL+GVE  LK++  ++ ENKV+ QS++YN LS S++A   +LYPLEYMFP+  LLPT +  AEQL  AP+PF+ G+P+SFL+ +K+F++P D+W  DLD        + K+ P T    ++   P  +  IL  H+KQAL    A N          S+ D  +  P+  + +V   ++  P                    +PS       NP ++G D DSVDV+TRVAMVRFFN+Q+ L N+ + TR L+LYPRP+VAFQ+++F++SR   S F+ + ++TQAV+F  EW++ P N  F ++ +   DP  +GDK KWF  +L PI F V+ +  S    L   +    +   PTDESGSDS+    SSSS   L++  S++A S +S                 Q L+  +DP  +Y+PP+ LQ P
Sbjct:  238 GGSHSPSPRASRKRTKLRNQSLTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVSDATPSIVLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPTPFVIGVPASFLVYKKNFRLPDDIWVVDLD--------STKLTPPTGGYEEIPPLPEPEGTILKNHLKQALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALTPIRFSVWDDGSSLNGALRSLKQLECQ---PTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPSLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYP 804          

HSP 2 Score: 229.18 bits (583), Expect = 7.671e-60
Identity = 121/319 (37.93%), Postives = 184/319 (57.68%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIE-PD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLIFRLFQ 4681
            R+YLF+ L GK DR  +W+ M FWED F DAV QER++IG+DQ   ++M+RY  L  +E++RLE +EDRLL  +L N  A ++M+ V   +I+ K R ++GKSHI L  S  ++ ++D I  L GND+DL    SR    Q F V+ G D  G +   E+  D ++LRS+ G I  RWW ER +  T   K  ++C+ RR   +  + + Y +K KEL   I++  E+  +    P+   +F V D  T   G L++   G+     N+ +FV   ++R+CFT+K   F++E  +P T+NL  R ++S  A+ I   F 
Sbjct: 1452 RVYLFEGLLGK-DRLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGTPTNVPELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNSKFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMASEIVLSFH 1769          

HSP 3 Score: 123.25 bits (308), Expect = 1.518e-27
Identity = 57/109 (52.29%), Postives = 75/109 (68.81%), Query Frame = 2
Query:  179 SICPRLIDYLSIVGLRN--PNRSGHKN-----SVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSR--NAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER 478
            S+CPRL+DY++IVG     P   G +        VP+LLRRYP +DH +  LP D+VYFCQP+GC S    R  +A +D  +FVF LTDK++G+ RYGIC+NF+RPIER
Sbjct:    8 SLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIER 116          

HSP 4 Score: 73.1738 bits (178), Expect = 2.452e-12
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query: 2519 KSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            KS   + P   RK ++ER+ LI   K +     +   +    +++   + ENQ  ++ VT+++L G     +   R K ++E+E+YR L+LS++NK L+K I   + +I+DV + ++  +  ++   + +  G++ ++      G  SVF  +E+  THY  K+
Sbjct: 1139 KSGINFDPFPGRKGLVERTPLI---KHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKKI-APDDHIDDVCV-TKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKE 1297          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Fly
Match: Rab3-GEF (gene:FBgn0030613 transcript:FBtr0307210)

HSP 1 Score: 404.06 bits (1037), Expect = 8.915e-115
Identity = 236/578 (40.83%), Postives = 339/578 (58.65%), Query Frame = 2
Query:  557 GGRESMSRQS--KRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALN----------SISD--KSEPVKNLDEVLNNVELQP-------------------LKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISEC---------------QRLT--VDPTKIYYPPTYLQIP 2122
            GG  S S ++  KR + R  SLTSL I+SHHP F  F+ CL ILK++I+  +E    +  G      +D++W  L G   + T S++ HDV EIETWI RLLS+PVPV G TR+E+ +L  T+ +P++FALPD+ RF LVDFPLH   EL+GVE  LK++  ++ ENKV+ QS++YN LS S++A   +LYPLEYMFP+  LLPT +  AEQL  AP+PF+ G+P+SFL+ +K+F++P D+W  DLD        + K+ P T    ++   P  +  IL  H+KQAL    A N          S+ D  +  P+  + +V   ++  P                    +PS       NP ++G D DSVDV+TRVAMVRFFN+Q+ L N+ + TR L+LYPRP+VAFQ+++F++SR   S F+ + ++TQAV+F  EW++ P N  F ++ +   DP  +GDK KWF  +L PI F V+ +  S    L   +    +   PTDESGSDS+    SSSS   L++  S++A S +S                 Q L+  +DP  +Y+PP+ LQ P
Sbjct:  238 GGSHSPSPRASRKRTKLRNQSLTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVSDATPSIVLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPTPFVIGVPASFLVYKKNFRLPDDIWVVDLD--------STKLTPPTGGYEEIPPLPEPEGTILKNHLKQALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALTPIRFSVWDDGSSLNGALRSLKQLECQ---PTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPSLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYP 804          

HSP 2 Score: 225.328 bits (573), Expect = 9.510e-59
Identity = 123/322 (38.20%), Postives = 188/322 (58.39%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIE-PD-TSQFKVTDDVTKCSGSLKI--DKNGLTFT-SDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLIFRLFQ 4681
            R+YLF+ L GK DR  +W+ M FWED F DAV QER++IG+DQ   ++M+RY  L  +E++RLE +EDRLL  +L N  A ++M+ V   +I+ K R ++GKSHI L  S  ++ ++D I  L GND+DL    SR    Q F V+ G D  G +   E+  D ++LRS+ G I  RWW ER +  T   K  ++C+ RR   +  + + Y +K KEL   I++  E+  +    P+   +F V D  T   G L++  +  GL F+ S + ++FV   ++R+CFT+K   F++E  +P T+NL  R ++S  A+ I   F 
Sbjct: 1452 RVYLFEGLLGK-DRLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGTPTNVPELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNSKDFEFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMASEIVLSFH 1772          

HSP 3 Score: 123.25 bits (308), Expect = 1.545e-27
Identity = 57/109 (52.29%), Postives = 75/109 (68.81%), Query Frame = 2
Query:  179 SICPRLIDYLSIVGLRN--PNRSGHKN-----SVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSR--NAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER 478
            S+CPRL+DY++IVG     P   G +        VP+LLRRYP +DH +  LP D+VYFCQP+GC S    R  +A +D  +FVF LTDK++G+ RYGIC+NF+RPIER
Sbjct:    8 SLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIER 116          

HSP 4 Score: 73.1738 bits (178), Expect = 2.313e-12
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query: 2519 KSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            KS   + P   RK ++ER+ LI   K +     +   +    +++   + ENQ  ++ VT+++L G     +   R K ++E+E+YR L+LS++NK L+K I   + +I+DV + ++  +  ++   + +  G++ ++      G  SVF  +E+  THY  K+
Sbjct: 1139 KSGINFDPFPGRKGLVERTPLI---KHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKKI-APDDHIDDVCV-TKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKE 1297          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Fly
Match: Rab3-GEF (gene:FBgn0030613 transcript:FBtr0307209)

HSP 1 Score: 405.216 bits (1040), Expect = 1.256e-114
Identity = 236/578 (40.83%), Postives = 339/578 (58.65%), Query Frame = 2
Query:  557 GGRESMSRQS--KRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALN----------SISD--KSEPVKNLDEVLNNVELQP-------------------LKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISEC---------------QRLT--VDPTKIYYPPTYLQIP 2122
            GG  S S ++  KR + R  SLTSL I+SHHP F  F+ CL ILK++I+  +E    +  G      +D++W  L G   + T S++ HDV EIETWI RLLS+PVPV G TR+E+ +L  T+ +P++FALPD+ RF LVDFPLH   EL+GVE  LK++  ++ ENKV+ QS++YN LS S++A   +LYPLEYMFP+  LLPT +  AEQL  AP+PF+ G+P+SFL+ +K+F++P D+W  DLD        + K+ P T    ++   P  +  IL  H+KQAL    A N          S+ D  +  P+  + +V   ++  P                    +PS       NP ++G D DSVDV+TRVAMVRFFN+Q+ L N+ + TR L+LYPRP+VAFQ+++F++SR   S F+ + ++TQAV+F  EW++ P N  F ++ +   DP  +GDK KWF  +L PI F V+ +  S    L   +    +   PTDESGSDS+    SSSS   L++  S++A S +S                 Q L+  +DP  +Y+PP+ LQ P
Sbjct:  246 GGSHSPSPRASRKRTKLRNQSLTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVSDATPSIVLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPTPFVIGVPASFLVYKKNFRLPDDIWVVDLD--------STKLTPPTGGYEEIPPLPEPEGTILKNHLKQALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALTPIRFSVWDDGSSLNGALRSLKQLECQ---PTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPSLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYP 812          

HSP 2 Score: 228.409 bits (581), Expect = 1.286e-59
Identity = 119/314 (37.90%), Postives = 183/314 (58.28%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIE-PD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            R+YLF+ L GK DR  +W+ M FWED F DAV QER++IG+DQ   ++M+RY  L  +E++RLE +EDRLL  +L N  A ++M+ V   +I+ K R ++GKSHI L  S  ++ ++D I  L GND+DL    SR    Q F V+ G D  G +   E+  D ++LRS+ G I  RWW ER +  T   K  ++C+ RR   +  + + Y +K KEL   I++  E+  +    P+   +F V D  T   G L++   G+     N+ +FV   ++R+CFT+K   F++E  +P T+NL  R ++S  ++ I
Sbjct: 1492 RVYLFEGLLGK-DRLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGTPTNVPELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNSKFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMSDQI 1804          

HSP 3 Score: 123.635 bits (309), Expect = 1.284e-27
Identity = 57/109 (52.29%), Postives = 75/109 (68.81%), Query Frame = 2
Query:  179 SICPRLIDYLSIVGLRN--PNRSGHKN-----SVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSR--NAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER 478
            S+CPRL+DY++IVG     P   G +        VP+LLRRYP +DH +  LP D+VYFCQP+GC S    R  +A +D  +FVF LTDK++G+ RYGIC+NF+RPIER
Sbjct:    8 SLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIER 116          

HSP 4 Score: 73.559 bits (179), Expect = 2.133e-12
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query: 2519 KSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            KS   + P   RK ++ER+ LI   K +     +   +    +++   + ENQ  ++ VT+++L G     +   R K ++E+E+YR L+LS++NK L+K I   + +I+DV + ++  +  ++   + +  G++ ++      G  SVF  +E+  THY  K+
Sbjct: 1147 KSGINFDPFPGRKGLVERTPLI---KHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKKI-APDDHIDDVCV-TKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKE 1305          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Fly
Match: Rab3-GEF (gene:FBgn0030613 transcript:FBtr0307208)

HSP 1 Score: 404.831 bits (1039), Expect = 1.547e-114
Identity = 236/578 (40.83%), Postives = 339/578 (58.65%), Query Frame = 2
Query:  557 GGRESMSRQS--KRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALN----------SISD--KSEPVKNLDEVLNNVELQP-------------------LKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISEC---------------QRLT--VDPTKIYYPPTYLQIP 2122
            GG  S S ++  KR + R  SLTSL I+SHHP F  F+ CL ILK++I+  +E    +  G      +D++W  L G   + T S++ HDV EIETWI RLLS+PVPV G TR+E+ +L  T+ +P++FALPD+ RF LVDFPLH   EL+GVE  LK++  ++ ENKV+ QS++YN LS S++A   +LYPLEYMFP+  LLPT +  AEQL  AP+PF+ G+P+SFL+ +K+F++P D+W  DLD        + K+ P T    ++   P  +  IL  H+KQAL    A N          S+ D  +  P+  + +V   ++  P                    +PS       NP ++G D DSVDV+TRVAMVRFFN+Q+ L N+ + TR L+LYPRP+VAFQ+++F++SR   S F+ + ++TQAV+F  EW++ P N  F ++ +   DP  +GDK KWF  +L PI F V+ +  S    L   +    +   PTDESGSDS+    SSSS   L++  S++A S +S                 Q L+  +DP  +Y+PP+ LQ P
Sbjct:  235 GGSHSPSPRASRKRTKLRNQSLTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVSDATPSIVLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPTPFVIGVPASFLVYKKNFRLPDDIWVVDLD--------STKLTPPTGGYEEIPPLPEPEGTILKNHLKQALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALTPIRFSVWDDGSSLNGALRSLKQLECQ---PTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPSLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYP 801          

HSP 2 Score: 228.409 bits (581), Expect = 1.580e-59
Identity = 119/314 (37.90%), Postives = 183/314 (58.28%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIE-PD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            R+YLF+ L GK DR  +W+ M FWED F DAV QER++IG+DQ   ++M+RY  L  +E++RLE +EDRLL  +L N  A ++M+ V   +I+ K R ++GKSHI L  S  ++ ++D I  L GND+DL    SR    Q F V+ G D  G +   E+  D ++LRS+ G I  RWW ER +  T   K  ++C+ RR   +  + + Y +K KEL   I++  E+  +    P+   +F V D  T   G L++   G+     N+ +FV   ++R+CFT+K   F++E  +P T+NL  R ++S  ++ I
Sbjct: 1498 RVYLFEGLLGK-DRLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGTPTNVPELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNSKFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMSDQI 1810          

HSP 3 Score: 123.635 bits (309), Expect = 1.375e-27
Identity = 57/109 (52.29%), Postives = 75/109 (68.81%), Query Frame = 2
Query:  179 SICPRLIDYLSIVGLRN--PNRSGHKN-----SVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSR--NAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER 478
            S+CPRL+DY++IVG     P   G +        VP+LLRRYP +DH +  LP D+VYFCQP+GC S    R  +A +D  +FVF LTDK++G+ RYGIC+NF+RPIER
Sbjct:    8 SLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIER 116          

HSP 4 Score: 73.1738 bits (178), Expect = 2.421e-12
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query: 2519 KSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            KS   + P   RK ++ER+ LI   K +     +   +    +++   + ENQ  ++ VT+++L G     +   R K ++E+E+YR L+LS++NK L+K I   + +I+DV + ++  +  ++   + +  G++ ++      G  SVF  +E+  THY  K+
Sbjct: 1153 KSGINFDPFPGRKGLVERTPLI---KHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKKI-APDDHIDDVCV-TKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKE 1311          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Fly
Match: Rab3-GEF (gene:FBgn0030613 transcript:FBtr0307207)

HSP 1 Score: 404.445 bits (1038), Expect = 2.008e-114
Identity = 236/578 (40.83%), Postives = 339/578 (58.65%), Query Frame = 2
Query:  557 GGRESMSRQS--KRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALN----------SISD--KSEPVKNLDEVLNNVELQP-------------------LKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISEC---------------QRLT--VDPTKIYYPPTYLQIP 2122
            GG  S S ++  KR + R  SLTSL I+SHHP F  F+ CL ILK++I+  +E    +  G      +D++W  L G   + T S++ HDV EIETWI RLLS+PVPV G TR+E+ +L  T+ +P++FALPD+ RF LVDFPLH   EL+GVE  LK++  ++ ENKV+ QS++YN LS S++A   +LYPLEYMFP+  LLPT +  AEQL  AP+PF+ G+P+SFL+ +K+F++P D+W  DLD        + K+ P T    ++   P  +  IL  H+KQAL    A N          S+ D  +  P+  + +V   ++  P                    +PS       NP ++G D DSVDV+TRVAMVRFFN+Q+ L N+ + TR L+LYPRP+VAFQ+++F++SR   S F+ + ++TQAV+F  EW++ P N  F ++ +   DP  +GDK KWF  +L PI F V+ +  S    L   +    +   PTDESGSDS+    SSSS   L++  S++A S +S                 Q L+  +DP  +Y+PP+ LQ P
Sbjct:  238 GGSHSPSPRASRKRTKLRNQSLTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVSDATPSIVLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPTPFVIGVPASFLVYKKNFRLPDDIWVVDLD--------STKLTPPTGGYEEIPPLPEPEGTILKNHLKQALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALTPIRFSVWDDGSSLNGALRSLKQLECQ---PTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPSLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYP 804          

HSP 2 Score: 228.409 bits (581), Expect = 1.547e-59
Identity = 119/314 (37.90%), Postives = 183/314 (58.28%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIE-PD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            R+YLF+ L GK DR  +W+ M FWED F DAV QER++IG+DQ   ++M+RY  L  +E++RLE +EDRLL  +L N  A ++M+ V   +I+ K R ++GKSHI L  S  ++ ++D I  L GND+DL    SR    Q F V+ G D  G +   E+  D ++LRS+ G I  RWW ER +  T   K  ++C+ RR   +  + + Y +K KEL   I++  E+  +    P+   +F V D  T   G L++   G+     N+ +FV   ++R+CFT+K   F++E  +P T+NL  R ++S  ++ I
Sbjct: 1452 RVYLFEGLLGK-DRLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGTPTNVPELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNSKFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMSDQI 1764          

HSP 3 Score: 123.635 bits (309), Expect = 1.376e-27
Identity = 57/109 (52.29%), Postives = 75/109 (68.81%), Query Frame = 2
Query:  179 SICPRLIDYLSIVGLRN--PNRSGHKN-----SVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSR--NAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER 478
            S+CPRL+DY++IVG     P   G +        VP+LLRRYP +DH +  LP D+VYFCQP+GC S    R  +A +D  +FVF LTDK++G+ RYGIC+NF+RPIER
Sbjct:    8 SLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIER 116          

HSP 4 Score: 73.1738 bits (178), Expect = 2.624e-12
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query: 2519 KSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            KS   + P   RK ++ER+ LI   K +     +   +    +++   + ENQ  ++ VT+++L G     +   R K ++E+E+YR L+LS++NK L+K I   + +I+DV + ++  +  ++   + +  G++ ++      G  SVF  +E+  THY  K+
Sbjct: 1139 KSGINFDPFPGRKGLVERTPLI---KHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKKI-APDDHIDDVCV-TKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKE 1297          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Zebrafish
Match: BX119977.1 (MAP-kinase activating death domain [Source:NCBI gene;Acc:100150917])

HSP 1 Score: 501.516 bits (1290), Expect = 9.406e-150
Identity = 265/590 (44.92%), Postives = 369/590 (62.54%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRP--RTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRR-----SNGKDSIWPTLLGS-SVENT--NSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G  RYGICLNF+R  ++       R   E     +         G+   S++S RI P  R   LTSL I+SH+P F  F+ CL ILK +++   +R+ +R     S  +D++W    G+ SVE     S +  D+ EIE+W++RLL SPVPVAG  R+++ +LP  L   + FALPD  RF +VDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P++ G+P+SF L +  FKMP DVW  DLD        N  +VP           P A  + L KH+KQ +    AL S+S  ++P+ NL++  +  EL  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  ++ F +KLS TQAV+++ EW + P N  F +I +  YDP LIGDK KW+   L+P+ ++VY  N
Sbjct:    2 MEKKKMCPRLLDYLVVVGARQP--SNDSVAQTPQLLRRYPLEDHNDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDTSFVFTLTDKDSGITRYGICLNFYRSFQKAH----HRPRAEGKGDGTLLPGEPGSSGKSPRSKRSGRIAPQNRNSMLTSLCILSHYPFFSTFRECLYILKRMVDCCSQRLNQRPGAPKSTQRDTMWRVFTGALSVEEKEKGSQVLQDLREIESWVYRLLRSPVPVAGQRRVDVEVLPHELQPALTFALPDPSRFSIVDFPLHLPLELLGVDACLQVLACILLEHKVVLQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYVIGVPASFFLYKCDFKMPDDVWLVDLDC-------NKVIVPSNAELLPPLPEPEA--SELKKHLKQ-VNAADALASMSLNTQPILNLEKFQDGQELSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRNGFTEKLSHTQAVEYYGEWALNPTNLAFQRIHNNVYDPSLIGDKPKWYAHQLQPVFYRVYDGN 575          

HSP 2 Score: 210.305 bits (534), Expect = 4.471e-54
Identity = 110/296 (37.16%), Postives = 173/296 (58.45%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVE 4600
            R+YL + L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+M+MM+V    I+ K R ++GKSHI L  S  IN+++D + +L G ++ +  + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E
Sbjct: 1152 RIYLCEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGDHDRKRLEDDEDRLLATLLHNMIAYMLMMKVGKNDIRKKVRRLMGKSHIGLTHSQEINEVLDRLAHLSGRELSIRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVK-GVFVLE 1445          

HSP 3 Score: 62.7734 bits (151), Expect = 5.020e-9
Identity = 32/113 (28.32%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L+G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV I +   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  904 ENQQFLKEVVQSVLEGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDAQQEVIRDVEI-NRKVYKGMLDILKCTVSSLEHSYTNAGLGGMASVFSLLEIARTHYQTK 1015          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Zebrafish
Match: madd (MAP-kinase activating death domain [Source:ZFIN;Acc:ZDB-GENE-030131-8076])

HSP 1 Score: 497.278 bits (1279), Expect = 3.559e-148
Identity = 265/594 (44.61%), Postives = 370/594 (62.29%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER--RSPIKVTRVXXXXXXXXXXXXXXXFQG--GRESMSRQSKRIRP--RTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRR-----SNGKDSIWPTLLGS-SVE--NTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G  RYGICLNF+R  ++    P    +       +     +    G  G+   S++S RI P  R   LTSL I+SH+P F  F+ CL ILK +++   +R+ +R     S  +D++W    G+ SVE     S +  D+ EIE+W++RLL SPVPVAG  R+++ +LP  L   + FALPD  RF +VDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P++ G+P+SF L +  FKMP DVW  DLD        N  +VP           P A  + L KH+KQAL       S+S  ++P+ NL++  +  EL  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  ++ F +KLS TQAV+++ EW + P N  F +I +  YDP LIGDK KW+   L+P+ ++VY  N
Sbjct:    1 MEKKKMCPRLLDYLVVVGARQP--SNDSVAQTPQLLRRYPLEDHNDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDTSFVFTLTDKDSGITRYGICLNFYRSFQKAHHRPRAEGKGEHSAPPAGDGTLLPGEPGSSGKSPRSKRSGRIAPQNRNSMLTSLCILSHYPFFSTFRECLYILKRMVDCCSQRLNQRPGAPKSTQRDTMWRVFTGALSVEEKEKGSQVLQDLREIESWVYRLLRSPVPVAGQRRVDVEVLPHELQPALTFALPDPSRFSIVDFPLHLPLELLGVDACLQVLACILLEHKVVLQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYVIGVPASFFLYKCDFKMPDDVWLVDLDC-------NKVIVPSNAELLPPLPEPEA--SELKKHLKQALA------SMSLNTQPILNLEKFQDGQELSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRNGFTEKLSHTQAVEYYGEWALNPTNLAFQRIHNNVYDPSLIGDKPKWYAHQLQPVFYRVYDGN 577          

HSP 2 Score: 219.55 bits (558), Expect = 6.754e-57
Identity = 123/339 (36.28%), Postives = 190/339 (56.05%), Query Frame = 2
Query: 3674 PVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            PV  N       VE  SM   R+YL + L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+M+MM+V    I+ K R ++GKSHI L  S  IN+++D + +L G ++ +  + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E   P TK +    +K+  A+ I
Sbjct: 1142 PVGVNKAAPAPPVEDVSM---RIYLCEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGDHDRKRLEDDEDRLLATLLHNMIAYMLMMKVGKNDIRKKVRRLMGKSHIGLTHSQEINEVLDRLAHLSGRELSIRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVK-GVFVLEEFVPETKEVVIHKYKTPMAHQI 1475          

HSP 3 Score: 62.7734 bits (151), Expect = 4.783e-9
Identity = 32/113 (28.32%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L+G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV I +   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  903 ENQQFLKEVVQSVLEGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDAQQEVIRDVEI-NRKVYKGMLDILKCTVSSLEHSYTNAGLGGMASVFSLLEIARTHYQTK 1014          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Zebrafish
Match: BX119977.1 (MAP-kinase activating death domain [Source:NCBI gene;Acc:100150917])

HSP 1 Score: 495.738 bits (1275), Expect = 5.928e-148
Identity = 265/594 (44.61%), Postives = 366/594 (61.62%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWR----PIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRP--RTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRR-----SNGKDSIWPTLLGS-SVENT--NSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G  RYGICLNF+R      E+  P  +T                         S++S RI P  R   LTSL I+SH+P F  F+ CL ILK +++   +R+ +R     S  +D++W    G+ SVE     S +  D+ EIE+W++RLL SPVPVAG  R+++ +LP  L   + FALPD  RF +VDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P++ G+P+SF L +  FKMP DVW  DLD        N  +VP           P A  + L KH+KQAL       S+S  ++P+ NL++  +  EL  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  ++ F +KLS TQAV+++ EW + P N  F +I +  YDP LIGDK KW+   L+P+ ++VY  N
Sbjct:    2 MEKKKMCPRLLDYLVVVGARQP--SNDSVAQTPQLLRRYPLEDHNDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDTSFVFTLTDKDSGITRYGICLNFYRSFQKATEKSDPSTLT-------------LSGEHSAPPSPRSKRSGRIAPQNRNSMLTSLCILSHYPFFSTFRECLYILKRMVDCCSQRLNQRPGAPKSTQRDTMWRVFTGALSVEEKEKGSQVLQDLREIESWVYRLLRSPVPVAGQRRVDVEVLPHELQPALTFALPDPSRFSIVDFPLHLPLELLGVDACLQVLACILLEHKVVLQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYVIGVPASFFLYKCDFKMPDDVWLVDLDC-------NKVIVPSNAELLPPLPEPEA--SELKKHLKQALA------SMSLNTQPILNLEKFQDGQELSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRNGFTEKLSHTQAVEYYGEWALNPTNLAFQRIHNNVYDPSLIGDKPKWYAHQLQPVFYRVYDGN 565          

HSP 2 Score: 211.075 bits (536), Expect = 2.470e-54
Identity = 117/317 (36.91%), Postives = 180/317 (56.78%), Query Frame = 2
Query: 3674 PVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVE 4600
            PV  N       VE  SM   R+YL + L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+M+MM+V    I+ K R ++GKSHI L  S  IN+++D + +L G ++ +  + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E
Sbjct: 1101 PVGVNKAAPAPPVEDVSM---RIYLCEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGDHDRKRLEDDEDRLLATLLHNMIAYMLMMKVGKNDIRKKVRRLMGKSHIGLTHSQEINEVLDRLAHLSGRELSIRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVK-GVFVLE 1412          

HSP 3 Score: 62.7734 bits (151), Expect = 4.633e-9
Identity = 32/113 (28.32%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L+G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV I +   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  798 ENQQFLKEVVQSVLEGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDAQQEVIRDVEI-NRKVYKGMLDILKCTVSSLEHSYTNAGLGGMASVFSLLEIARTHYQTK 909          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Zebrafish
Match: BX119977.1 (MAP-kinase activating death domain [Source:NCBI gene;Acc:100150917])

HSP 1 Score: 496.508 bits (1277), Expect = 2.564e-147
Identity = 265/608 (43.59%), Postives = 377/608 (62.01%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWR----------PIERRSPIKVTRVXXXXXXXXXXXXXXXFQG--------GRESMSRQSKRIRP--RTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRR-----SNGKDSIWPTLLGS-SVENT--NSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G  RYGICLNF+R          P    + ++ T  S     + S    +   G        G+   S++S RI P  R   LTSL I+SH+P F  F+ CL ILK +++   +R+ +R     S  +D++W    G+ SVE     S +  D+ EIE+W++RLL SPVPVAG  R+++ +LP  L   + FALPD  RF +VDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P++ G+P+SF L +  FKMP DVW  DLD  +++  +N +++P              + + L KH+KQAL       S+S  ++P+ NL++  +  EL  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  ++ F +KLS TQAV+++ EW + P N  F +I +  YDP LIGDK KW+   L+P+ ++VY  N
Sbjct:    2 MEKKKMCPRLLDYLVVVGARQP--SNDSVAQTPQLLRRYPLEDHNDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDTSFVFTLTDKDSGITRYGICLNFYRSFQKAHHREKPAHTDTAVEATEKSDPSTLTLSGEHSAPPAGDGTLLPGDGKSPRSKRSGRIAPQNRNSMLTSLCILSHYPFFSTFRECLYILKRMVDCCSQRLNQRPGAPKSTQRDTMWRVFTGALSVEEKEKGSQVLQDLREIESWVYRLLRSPVPVAGQRRVDVEVLPHELQPALTFALPDPSRFSIVDFPLHLPLELLGVDACLQVLACILLEHKVVLQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYVIGVPASFFLYKCDFKMPDDVWLVDLDCNKVIVPSNAELLPPLPE---------PEASELKKHLKQALA------SMSLNTQPILNLEKFQDGQELSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRNGFTEKLSHTQAVEYYGEWALNPTNLAFQRIHNNVYDPSLIGDKPKWYAHQLQPVFYRVYDGN 592          

HSP 2 Score: 211.846 bits (538), Expect = 1.399e-54
Identity = 117/317 (36.91%), Postives = 180/317 (56.78%), Query Frame = 2
Query: 3674 PVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVE 4600
            PV  N       VE  SM   R+YL + L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+M+MM+V    I+ K R ++GKSHI L  S  IN+++D + +L G ++ +  + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E
Sbjct: 1222 PVGVNKAAPAPPVEDVSM---RIYLCEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGDHDRKRLEDDEDRLLATLLHNMIAYMLMMKVGKNDIRKKVRRLMGKSHIGLTHSQEINEVLDRLAHLSGRELSIRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVK-GVFVLE 1533          

HSP 3 Score: 62.7734 bits (151), Expect = 4.402e-9
Identity = 32/113 (28.32%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L+G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV I +   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  915 ENQQFLKEVVQSVLEGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDAQQEVIRDVEI-NRKVYKGMLDILKCTVSSLEHSYTNAGLGGMASVFSLLEIARTHYQTK 1026          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Zebrafish
Match: BX119977.1 (MAP-kinase activating death domain [Source:NCBI gene;Acc:100150917])

HSP 1 Score: 493.041 bits (1268), Expect = 5.171e-147
Identity = 262/605 (43.31%), Postives = 376/605 (62.15%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER--RSPIKVTRVXXXXXXXXXXXXXXXFQG--GRESMSRQSKRIRP--RTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRR-----SNGKDSIWPTLLGS-SVE--NTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQ-----------ALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G  RYGICLNF+R  ++    P    +       +     +    G  G+   S++S RI P  R   LTSL I+SH+P F  F+ CL ILK +++   +R+ +R     S  +D++W    G+ SVE     S +  D+ EIE+W++RLL SPVPVAG  R+++ +LP  L   + FALPD  RF +VDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P++ G+P+SF L +  FKMP DVW  DLD  +++  +N +++P              + + L KH+KQ           +  +  AL S+S  ++P+ NL++  +  EL  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  ++ F +KLS TQAV+++ EW + P N  F +I +  YDP LIGDK KW+   L+P+ ++VY  N
Sbjct:    2 MEKKKMCPRLLDYLVVVGARQP--SNDSVAQTPQLLRRYPLEDHNDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDTSFVFTLTDKDSGITRYGICLNFYRSFQKAHHRPRAEGKGEHSAPPAGDGTLLPGEPGSSGKSPRSKRSGRIAPQNRNSMLTSLCILSHYPFFSTFRECLYILKRMVDCCSQRLNQRPGAPKSTQRDTMWRVFTGALSVEEKEKGSQVLQDLREIESWVYRLLRSPVPVAGQRRVDVEVLPHELQPALTFALPDPSRFSIVDFPLHLPLELLGVDACLQVLACILLEHKVVLQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYVIGVPASFFLYKCDFKMPDDVWLVDLDCNKVIVPSNAELLPPLPE---------PEASELKKHLKQVNAADVLISLRSPTINCALASMSLNTQPILNLEKFQDGQELSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRNGFTEKLSHTQAVEYYGEWALNPTNLAFQRIHNNVYDPSLIGDKPKWYAHQLQPVFYRVYDGN 595          

HSP 2 Score: 202.601 bits (514), Expect = 1.022e-51
Identity = 111/289 (38.41%), Postives = 164/289 (56.75%), Query Frame = 2
Query: 3674 PVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTF 4516
            PV  N       VE  SM   R+YL + L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+M+MM+V    I+ K R ++GKSHI L  S  IN+++D + +L G ++ +  + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+  
Sbjct: 1161 PVGVNKAAPAPPVEDVSM---RIYLCEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGDHDRKRLEDDEDRLLATLLHNMIAYMLMMKVGKNDIRKKVRRLMGKSHIGLTHSQEINEVLDRLAHLSGRELSIRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINL 1445          

HSP 3 Score: 62.7734 bits (151), Expect = 5.039e-9
Identity = 32/113 (28.32%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L+G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV I +   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  922 ENQQFLKEVVQSVLEGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDAQQEVIRDVEI-NRKVYKGMLDILKCTVSSLEHSYTNAGLGGMASVFSLLEIARTHYQTK 1033          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Xenopus
Match: madd (MAP-kinase activating death domain [Source:NCBI gene;Acc:780174])

HSP 1 Score: 504.212 bits (1297), Expect = 4.953e-150
Identity = 272/598 (45.48%), Postives = 372/598 (62.21%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRE------------SMSRQSKRI-----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLK-PSQSE---ISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL I+G R P  S    S  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G +RYGIC+NF+R  ++R P   +R   +      S       GG              + S + KR+     R R  +LTSL I+SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  +E   +V+ HD+ EIE WI+RLL SP PV G  R+++ +LP     P+ FALPD  RF LVDFPLH   EL+GVE  L++   ++LE+KV++QS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD         +K+V  TN D    LP P + + L KH+KQAL       S+S  ++P+ NLD      +   LK PS ++    + FNPLI+GND DSVDV+TRVAMVRFFNS ++L ++   TR L+L+PRP+VAFQ  +F+ +R   S F +KLSKTQAV+FF EW++ P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIIGARQP--SSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGS---DGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLD--------TNKVVAPTNADLLPVLPEP-EASELKKHLKQALA------SMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDAN 585          

HSP 2 Score: 211.846 bits (538), Expect = 2.429e-54
Identity = 113/314 (35.99%), Postives = 177/314 (56.37%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+MIMM+V+   ++ K R ++GK HI L  S  IN+L+D +  + G D+ +  + SR    Q F V+ G D  GD+   E+  DC++LRS  GA+  RWW E+ I  T   K  V+C+ RR   E  +++ + KK   L      H      V      +F V D  T   G L++   G+     ++  F+   ++++C T +   F++E   P TK +    +K+  A+ I
Sbjct: 1222 SQRIYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKVTGLF-----HVYWLGQVGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVR-GVFVLEEFVPETKEVVSHKYKTPMAHEI 1528          

HSP 3 Score: 57.3806 bits (137), Expect = 3.043e-7
Identity = 28/114 (24.56%), Postives = 63/114 (55.26%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            ENQ+ +++V   +L+G     ++  +++ ++E+E  R  +LS++N++++   + +   I +V I +   +  ++ + +  V+ +E +Y      G  SVF  LE+  THY  K+
Sbjct:  909 ENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEI-TRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKE 1021          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Xenopus
Match: madd (MAP-kinase activating death domain [Source:NCBI gene;Acc:780174])

HSP 1 Score: 504.982 bits (1299), Expect = 7.611e-150
Identity = 272/598 (45.48%), Postives = 372/598 (62.21%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRE------------SMSRQSKRI-----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLK-PSQSE---ISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL I+G R P  S    S  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G +RYGIC+NF+R  ++R P   +R   +      S       GG              + S + KR+     R R  +LTSL I+SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  +E   +V+ HD+ EIE WI+RLL SP PV G  R+++ +LP     P+ FALPD  RF LVDFPLH   EL+GVE  L++   ++LE+KV++QS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD         +K+V  TN D    LP P + + L KH+KQAL       S+S  ++P+ NLD      +   LK PS ++    + FNPLI+GND DSVDV+TRVAMVRFFNS ++L ++   TR L+L+PRP+VAFQ  +F+ +R   S F +KLSKTQAV+FF EW++ P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIIGARQP--SSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGS---DGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLD--------TNKVVAPTNADLLPVLPEP-EASELKKHLKQALA------SMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDAN 585          

HSP 2 Score: 211.46 bits (537), Expect = 3.058e-54
Identity = 114/340 (33.53%), Postives = 187/340 (55.00%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+MIMM+V+   ++ K R ++GK HI L  S  IN+L+D +  + G D+ +  + SR    Q F V+ G D  GD+   E+  DC++LRS  GA+  RWW E+ I  T   K  V+C+ RR   E  +++ + KK +EL   +++  E+    +  I+P      +F V D  T   G L++   G+     ++  F+   ++++C T +   F++E   P TK +    +K+  A+ I
Sbjct: 1261 SQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVR-GVFVLEEFVPETKEVVSHKYKTPMAHEI 1599          

HSP 3 Score: 57.3806 bits (137), Expect = 2.866e-7
Identity = 28/114 (24.56%), Postives = 63/114 (55.26%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            ENQ+ +++V   +L+G     ++  +++ ++E+E  R  +LS++N++++   + +   I +V I +   +  ++ + +  V+ +E +Y      G  SVF  LE+  THY  K+
Sbjct:  927 ENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEI-TRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKE 1039          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Xenopus
Match: madd (MAP-kinase activating death domain [Source:NCBI gene;Acc:780174])

HSP 1 Score: 504.597 bits (1298), Expect = 7.747e-150
Identity = 272/598 (45.48%), Postives = 372/598 (62.21%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRE------------SMSRQSKRI-----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLK-PSQSE---ISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL I+G R P  S    S  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G +RYGIC+NF+R  ++R P   +R   +      S       GG              + S + KR+     R R  +LTSL I+SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  +E   +V+ HD+ EIE WI+RLL SP PV G  R+++ +LP     P+ FALPD  RF LVDFPLH   EL+GVE  L++   ++LE+KV++QS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD         +K+V  TN D    LP P + + L KH+KQAL       S+S  ++P+ NLD      +   LK PS ++    + FNPLI+GND DSVDV+TRVAMVRFFNS ++L ++   TR L+L+PRP+VAFQ  +F+ +R   S F +KLSKTQAV+FF EW++ P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIIGARQP--SSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGS---DGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLD--------TNKVVAPTNADLLPVLPEP-EASELKKHLKQALA------SMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDAN 585          

HSP 2 Score: 204.527 bits (519), Expect = 4.363e-52
Identity = 112/334 (33.53%), Postives = 178/334 (53.29%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+MIMM+V+   ++ K R ++GK HI L  S  IN+L+D +  + G D+ +  + SR    Q F V+ G D  GD+   E+  DC++LRS  GA+  RWW E+ I  T   K  V+C+ RR   E  +++ + KK   L      H      V      +F V D  T   G L++   G+     ++  F+   ++++C T +   F++E   P TK +    +K+  A+ I
Sbjct: 1258 SQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKVTGLF-----HVYWLGQVGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVR-GVFVLEEFVPETKEVVSHKYKTPMAHEI 1585          

HSP 3 Score: 57.3806 bits (137), Expect = 2.862e-7
Identity = 28/114 (24.56%), Postives = 63/114 (55.26%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            ENQ+ +++V   +L+G     ++  +++ ++E+E  R  +LS++N++++   + +   I +V I +   +  ++ + +  V+ +E +Y      G  SVF  LE+  THY  K+
Sbjct:  927 ENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEI-TRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKE 1039          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Xenopus
Match: madd (MAP-kinase activating death domain [Source:NCBI gene;Acc:780174])

HSP 1 Score: 502.671 bits (1293), Expect = 1.414e-149
Identity = 273/611 (44.68%), Postives = 374/611 (61.21%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRE------------SMSRQSKRI-----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVC-------------QALNSISDKSEPVKNLDEVLNNVELQPLK-PSQSE---ISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL I+G R P  S    S  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G +RYGIC+NF+R  ++R P   +R   +      S       GG              + S + KR+     R R  +LTSL I+SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  +E   +V+ HD+ EIE WI+RLL SP PV G  R+++ +LP     P+ FALPD  RF LVDFPLH   EL+GVE  L++   ++LE+KV++QS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD         +K+V  TN D    LP P + + L KH+KQ L                +AL S+S  ++P+ NLD      +   LK PS ++    + FNPLI+GND DSVDV+TRVAMVRFFNS ++L ++   TR L+L+PRP+VAFQ  +F+ +R   S F +KLSKTQAV+FF EW++ P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIIGARQP--SSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGS---DGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLD--------TNKVVAPTNADLLPVLPEP-EASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDAN 604          

HSP 2 Score: 210.69 bits (535), Expect = 4.372e-54
Identity = 114/340 (33.53%), Postives = 187/340 (55.00%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+MIMM+V+   ++ K R ++GK HI L  S  IN+L+D +  + G D+ +  + SR    Q F V+ G D  GD+   E+  DC++LRS  GA+  RWW E+ I  T   K  V+C+ RR   E  +++ + KK +EL   +++  E+    +  I+P      +F V D  T   G L++   G+     ++  F+   ++++C T +   F++E   P TK +    +K+  A+ I
Sbjct: 1182 SQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVR-GVFVLEEFVPETKEVVSHKYKTPMAHEI 1520          

HSP 3 Score: 56.9954 bits (136), Expect = 4.118e-7
Identity = 28/113 (24.78%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L+G     ++  +++ ++E+E  R  +LS++N++++   + +   I +V I +   +  ++ + +  V+ +E +Y      G  SVF  LE+  THY  K
Sbjct:  932 ENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEI-TRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNK 1043          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Xenopus
Match: madd (MAP-kinase activating death domain [Source:NCBI gene;Acc:780174])

HSP 1 Score: 503.056 bits (1294), Expect = 1.503e-149
Identity = 273/611 (44.68%), Postives = 374/611 (61.21%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRE------------SMSRQSKRI-----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVC-------------QALNSISDKSEPVKNLDEVLNNVELQPLK-PSQSE---ISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL I+G R P  S    S  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK++G +RYGIC+NF+R  ++R P   +R   +      S       GG              + S + KR+     R R  +LTSL I+SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  +E   +V+ HD+ EIE WI+RLL SP PV G  R+++ +LP     P+ FALPD  RF LVDFPLH   EL+GVE  L++   ++LE+KV++QS++YN LS S++A  +++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD         +K+V  TN D    LP P + + L KH+KQ L                +AL S+S  ++P+ NLD      +   LK PS ++    + FNPLI+GND DSVDV+TRVAMVRFFNS ++L ++   TR L+L+PRP+VAFQ  +F+ +R   S F +KLSKTQAV+FF EW++ P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIIGARQP--SSDSISQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQKRMSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPASENGSEGS---DGGHPMQPHSAESTPDINQSPRGKRLSKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIFTGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVLPLDHQPPLTFALPDPSRFSLVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLD--------TNKVVAPTNADLLPVLPEP-EASELKKHLKQCLHSMSVITQQRLLASDNKALASMSLNTQPILNLDRFHEGPDAALLKAPSDAQGTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQMHTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDAN 604          

HSP 2 Score: 211.075 bits (536), Expect = 4.021e-54
Identity = 114/340 (33.53%), Postives = 187/340 (55.00%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+MIMM+V+   ++ K R ++GK HI L  S  IN+L+D +  + G D+ +  + SR    Q F V+ G D  GD+   E+  DC++LRS  GA+  RWW E+ I  T   K  V+C+ RR   E  +++ + KK +EL   +++  E+    +  I+P      +F V D  T   G L++   G+     ++  F+   ++++C T +   F++E   P TK +    +K+  A+ I
Sbjct: 1200 SQRIYLYEGLLGRDKGSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDHDRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDMNGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIQLNHIKKCNTVR-GVFVLEEFVPETKEVVSHKYKTPMAHEI 1538          

HSP 3 Score: 57.3806 bits (137), Expect = 3.178e-7
Identity = 28/114 (24.56%), Postives = 63/114 (55.26%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            ENQ+ +++V   +L+G     ++  +++ ++E+E  R  +LS++N++++   + +   I +V I +   +  ++ + +  V+ +E +Y      G  SVF  LE+  THY  K+
Sbjct:  932 ENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRSIQTEEDARNDVIQEVEI-TRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHTHYYNKE 1044          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Mouse
Match: Madd (MAP-kinase activating death domain [Source:MGI Symbol;Acc:MGI:2444672])

HSP 1 Score: 506.138 bits (1302), Expect = 8.478e-155
Identity = 277/632 (43.83%), Postives = 383/632 (60.60%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXX----XXXXXXXXXXXXXFQGGRESMS-----------------RQSKRI----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSS-VENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD 1948
            M  +  CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R P +          +     S A      G  ES S                 R  +R     R R  +LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S +  D+ EIE WI+RLL SPVPV+G  R+++ +LP+ L + + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N      L    + +++SD
Sbjct:    1 MVQKKFCPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSD 615          

HSP 2 Score: 61.2326 bits (147), Expect = 1.537e-8
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  911 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD 1050          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Mouse
Match: Madd (MAP-kinase activating death domain [Source:MGI Symbol;Acc:MGI:2444672])

HSP 1 Score: 509.605 bits (1311), Expect = 8.891e-153
Identity = 277/632 (43.83%), Postives = 383/632 (60.60%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXX----XXXXXXXXXXXXXFQGGRESMS-----------------RQSKRI----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSS-VENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD 1948
            M  +  CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R P +          +     S A      G  ES S                 R  +R     R R  +LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S +  D+ EIE WI+RLL SPVPV+G  R+++ +LP+ L + + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N      L    + +++SD
Sbjct:    1 MVQKKFCPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSD 615          

HSP 2 Score: 204.527 bits (519), Expect = 2.874e-52
Identity = 103/270 (38.15%), Postives = 162/270 (60.00%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTF 4516
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  +N+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+  
Sbjct: 1194 SQRVYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINL 1462          

HSP 3 Score: 60.077 bits (144), Expect = 3.572e-8
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  868 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD 1007          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Mouse
Match: Madd (MAP-kinase activating death domain [Source:MGI Symbol;Acc:MGI:2444672])

HSP 1 Score: 509.99 bits (1312), Expect = 3.472e-152
Identity = 277/632 (43.83%), Postives = 383/632 (60.60%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXX----XXXXXXXXXXXXXFQGGRESMS-----------------RQSKRI----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSS-VENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD 1948
            M  +  CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R P +          +     S A      G  ES S                 R  +R     R R  +LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S +  D+ EIE WI+RLL SPVPV+G  R+++ +LP+ L + + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N      L    + +++SD
Sbjct:    1 MVQKKFCPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSD 615          

HSP 2 Score: 196.823 bits (499), Expect = 6.878e-50
Identity = 102/290 (35.17%), Postives = 163/290 (56.21%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTF 4516
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  +N+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+  
Sbjct: 1256 SQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINL 1545          

HSP 3 Score: 60.077 bits (144), Expect = 3.765e-8
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  911 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD 1050          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Mouse
Match: Madd (MAP-kinase activating death domain [Source:MGI Symbol;Acc:MGI:2444672])

HSP 1 Score: 509.22 bits (1310), Expect = 3.783e-152
Identity = 277/632 (43.83%), Postives = 383/632 (60.60%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXX----XXXXXXXXXXXXXFQGGRESMS-----------------RQSKRI----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSS-VENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD 1948
            M  +  CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R P +          +     S A      G  ES S                 R  +R     R R  +LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S +  D+ EIE WI+RLL SPVPV+G  R+++ +LP+ L + + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N      L    + +++SD
Sbjct:    1 MVQKKFCPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSD 615          

HSP 2 Score: 216.468 bits (550), Expect = 6.454e-56
Identity = 113/320 (35.31%), Postives = 186/320 (58.13%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  +N+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     +   F++  ++++C T +   F++E   P  K +    +K+  A+ I
Sbjct: 1194 SQRVYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEI 1511          

HSP 3 Score: 59.6918 bits (143), Expect = 4.373e-8
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  868 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD 1007          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Mouse
Match: Madd (MAP-kinase activating death domain [Source:MGI Symbol;Acc:MGI:2444672])

HSP 1 Score: 509.605 bits (1311), Expect = 3.941e-152
Identity = 277/632 (43.83%), Postives = 383/632 (60.60%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXX----XXXXXXXXXXXXXFQGGRESMS-----------------RQSKRI----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSS-VENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD 1948
            M  +  CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R P +          +     S A      G  ES S                 R  +R     R R  +LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S +  D+ EIE WI+RLL SPVPV+G  R+++ +LP+ L + + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N      L    + +++SD
Sbjct:    1 MVQKKFCPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSD 615          

HSP 2 Score: 216.468 bits (550), Expect = 6.955e-56
Identity = 113/320 (35.31%), Postives = 186/320 (58.13%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L GK +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  +N+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     +   F++  ++++C T +   F++E   P  K +    +K+  A+ I
Sbjct: 1215 SQRVYLYEGLLGK-ERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEI 1532          

HSP 3 Score: 59.6918 bits (143), Expect = 5.017e-8
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  888 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD 1027          
BLAST of MAP kinase-activating death domain protein vs. UniProt/SwissProt
Match: sp|Q80U28|MADD_MOUSE (MAP kinase-activating death domain protein OS=Mus musculus OX=10090 GN=Madd PE=1 SV=2)

HSP 1 Score: 509.605 bits (1311), Expect = 4.314e-151
Identity = 274/632 (43.35%), Postives = 380/632 (60.13%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQ----GGRESMS---------------------RQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSS-VENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD 1948
            M  +  CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R P +             +      +    G  ES S                     R     R R  +LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S +  D+ EIE WI+RLL SPVPV+G  R+++ +LP+ L + + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N      L    + +++SD
Sbjct:    1 MVQKKFCPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAATGSSESGSTLQPPSADSTPDINQSPWGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSD 615          

HSP 2 Score: 196.823 bits (499), Expect = 4.807e-49
Identity = 102/290 (35.17%), Postives = 163/290 (56.21%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTF 4516
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  +N+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+  
Sbjct: 1275 SQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINL 1564          

HSP 3 Score: 60.077 bits (144), Expect = 2.662e-7
Identity = 35/141 (24.82%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  931 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD 1070          
BLAST of MAP kinase-activating death domain protein vs. UniProt/SwissProt
Match: sp|O08873|MADD_RAT (MAP kinase-activating death domain protein OS=Rattus norvegicus OX=10116 GN=Madd PE=1 SV=1)

HSP 1 Score: 508.064 bits (1307), Expect = 2.406e-150
Identity = 267/633 (42.18%), Postives = 376/633 (59.40%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRP-------------------------RTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSS-VENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQ-------SEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD 1948
            M  +  CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R P +             +      +      S     ++P                         R  +LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S + HD+ EIE WI+RLL SPVPV+G  R+++ +LP+ + + + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E  PL   +       +  + FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N      L    + ++ESD
Sbjct:    1 MVQKKFCPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKAEGGAGPRGKEGAHAPCASEEAATESSESGSTLQPPSADSTPDVNQSPRGKRRAKAGNRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKPGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQEVQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQE-TPLLLGRFSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSESD 615          

HSP 2 Score: 208.379 bits (529), Expect = 1.491e-52
Identity = 112/340 (32.94%), Postives = 187/340 (55.00%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSH+ L  S  IN+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     +   F++  ++++C T +   F++E   P  K +    +K+  A+ I
Sbjct: 1234 SQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHVGLVYSQQINEVLDQLTNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEI 1572          

HSP 3 Score: 57.7658 bits (138), Expect = 1.166e-6
Identity = 34/141 (24.11%), Postives = 70/141 (49.65%), Query Frame = 2
Query: 2645 KDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            + + S   ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++++ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  911 QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLSRAVQSEDDARQDVIQDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD 1050          
BLAST of MAP kinase-activating death domain protein vs. UniProt/SwissProt
Match: sp|Q8WXG6|MADD_HUMAN (MAP kinase-activating death domain protein OS=Homo sapiens OX=9606 GN=MADD PE=1 SV=2)

HSP 1 Score: 498.049 bits (1281), Expect = 1.600e-146
Identity = 269/608 (44.24%), Postives = 370/608 (60.86%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR-----------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHS--------LTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPL--KPS---QSEISG-FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
            CPRL+DYL IVG R+P  S    +  PELLRRYP  DH    LP DVV+FCQP+GC+S    R + +D  +FVFTLTDK+TG  RYGIC+NF+R  ++R                 +             S S + +            QS R + R  +        LTSL ++SH+P F  F+ CL  LK +++   ER+  +  G      +D++W    GS  VE  +S + HD+ EIE WI+RLL SPVPV+G  R+++ +LP+ L   + FALPD  RF LVDFPLH   EL+GV+  L++   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++   N +++P         LP P     L KH+KQAL       S+S  ++P+ NL++     E+  L  +PS   QS  S  FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  Q+ F +KL++TQAV++F EW + P N  F +I +  +DP LIGDK KW+   L+PI+++VY  N
Sbjct:    8 CPRLLDYLVIVGARHP--SSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLP--------ILPEPESLE-LKKHLKQALA------SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSN 598          

HSP 2 Score: 208.764 bits (530), Expect = 1.094e-52
Identity = 113/340 (33.24%), Postives = 187/340 (55.00%), Query Frame = 2
Query: 3731 NQRMYLFQKLTGKN--------------------DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +QR+YL++ L G++                    +RS +WD M FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N I++M++M+V+   I+ K R ++GKSHI L  S  IN+++D +  L G D+ + ++ SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR  +E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     +   F++  ++++C T +   F++E   P  K +    +K+  A+ I
Sbjct: 1279 SQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEI 1617          

HSP 3 Score: 58.5362 bits (140), Expect = 7.248e-7
Identity = 34/133 (25.56%), Postives = 67/133 (50.38%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTND 3067
            ENQ+ +++V   +L G     ++  +++ ++E+E  R  +LS++N+ ++   + ++  I DV I S   +  ++ + +  V  +E SY      G  S+F  LE+  THY  K+      S +    +PV  D
Sbjct:  940 ENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI-SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKD 1071          
BLAST of MAP kinase-activating death domain protein vs. UniProt/SwissProt
Match: sp|O02626|MADD_CAEEL (MAP kinase-activating death domain protein OS=Caenorhabditis elegans OX=6239 GN=aex-3 PE=1 SV=1)

HSP 1 Score: 437.573 bits (1124), Expect = 5.849e-127
Identity = 243/593 (40.98%), Postives = 340/593 (57.34%), Query Frame = 2
Query:  182 ICPRLIDYLSIVGLRNPNRSGHKNS---------VVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTS--RNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIK--VTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTH-SLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLP--PPADFNILIKHIKQALKVCQALNSIS----DKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            ICPRLID+L +VG RN  R   ++S           PE+LRRYP  DHK+  LP DV  FCQP+GC +T     +NA  D   FVF LT+K++ +VRYGICLNF++  +RRS  K  + +V  +                    +   KR    +H SLTSL  +SHHP    F   L +LK II+  + R  +R+  KD +W  L G   E     +  ++ EIETWI  LLSSPVPV G T++++ ++P  L +   FALPD+ RF L+DFPLH  FE++G++ AL++  A +LE K+++QS+NYN +S  IL++ ALLYPLEYMFP+  LLP +MPSAEQL  AP+PF+ G+PSSF   RK  ++PSDV   DLD   L             + +DL +P  P  D      H+K+ LK   A+N ++    D    V + D                         FG D DSVDV+ RVAMV+FFNS ++ GN+++ TR L+LYPRP+V+ Q D+F++SR   +  +  L +TQAV++F E  + PKN  F ++ +       +GDK KWF +SL P++F VY  N +
Sbjct:    8 ICPRLIDFLVVVGKRNRTRGASQSSPDATTDTTVTYPEILRRYPTDDHKDFILPTDVTVFCQPEGCTTTSARLRKNARNDPQFFVFMLTEKDSAKVRYGICLNFYQSFDRRSTPKDEIKKVPDD--------------------AHHKKR---DSHVSLTSLCFISHHPFVSIFHQVLLLLKRIIDSSNHRAAQRTGLKDVVWAILTGHYNEPIVPEVMKEIKEIETWILMLLSSPVPVPGKTKVQIEVMPMDLSQVFEFALPDHTRFTLIDFPLHIPFEILGIDMALRVLTAAMLEFKIVIQSRNYNAVSMCILSIVALLYPLEYMFPVIPLLPAYMPSAEQLLLAPTPFLIGVPSSFFHHRKIRELPSDVILVDLDTNCL------------QVPDDLYIPDLPEPDAT----HLKERLK--NAINKMTTMTVDNETSVTDAD-------------------------FGIDIDSVDVACRVAMVQFFNSANVFGNFSEHTRTLRLYPRPVVSLQTDSFLRSRPQCTQLITDLCRTQAVEYFAECCLCPKNETFVRVQAGIESAEQVGDKPKWFSESLMPVHFMVYPNNST 534          

HSP 2 Score: 196.052 bits (497), Expect = 7.743e-49
Identity = 109/318 (34.28%), Postives = 176/318 (55.35%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQ----AIQKHSEKFRSVIEPDT--SQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            R Y++Q+L     + +IW N+ FWE+ F D V QEREI+G+DQ+  +++DRY+ L+ +EK+RLE  EDRLL  +L+N  A+MIM       ++ K R ++GK+HI L  S  INKL+D +   +GN + L    SR    Q F V  G    G +   E+  D ++LRSI+GA   RWW ER +  T   K  ++C+ RR +++V M + + KK +EL Q    A+++ + + +  +E      +F V D  T   G L++  +G+     +   F+   N+++C T   + F++E  D     +  R + S  A+ I
Sbjct: 1071 RHYIYQELILAV-QHQIWQNLQFWENAFVDLVAQEREIVGMDQEPSEMIDRYSALNDSEKKRLELEEDRLLSTLLHNMTAYMIMCGTGQKALQQKVRRLLGKAHIGLVCSKEINKLLDELPSTQGNFIPLKPLGSRLVQKQSFTVCPGQSSDGQMMFMEVCDDAVVLRSITGAATERWWYERLVNITYSPKTKILCLWRRHDDKVHMHKFHTKKCRELYQCMKAAMERAAARGKVNVEGRALGGEFPVHDTETNQGGLLQVRCDGVAVIFAHNQIFIGLSNIKKCNTFGGNVFLLEEFDRKKGEIIQRRYFSQMADQI 1387          

HSP 3 Score: 76.6406 bits (187), Expect = 2.178e-12
Identity = 50/171 (29.24%), Postives = 92/171 (53.80%), Query Frame = 2
Query: 2507 QEILKSNPTYKPISER--KPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKG 3013
            Q I K+ P  + + ER  KP      L N+++++    R  +  +   +   +   +NQ+T+++  D+ L G +  + S  ++K ++E+E+ R L+ S++N  LE  ++ ++ Y+ +V + ++  F A V I +  + GIE S+N   C GF SVF  LE+  THY    G
Sbjct:  733 QVINKTAPKAQALKERTMKP------LANRIEQSQHIVRSKTQPNPTSQQTANQQSKNQQTVKEFCDQALVGQSVGMFSAPKLKRLMEDESLRELVCSKLNLGLEVKLS-EDEYVKEVQL-TKGQFKAYVKILKACLEGIEVSFNTPGCCGFASVFHVLEIAHTHYWAMGG 895          
BLAST of MAP kinase-activating death domain protein vs. UniProt/SwissProt
Match: sp|Q9VXY2|MADD_DROME (MAP kinase-activating death domain protein OS=Drosophila melanogaster OX=7227 GN=Rab3-GEF PE=2 SV=2)

HSP 1 Score: 404.445 bits (1038), Expect = 2.013e-113
Identity = 236/578 (40.83%), Postives = 339/578 (58.65%), Query Frame = 2
Query:  557 GGRESMSRQS--KRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG------KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALN----------SISD--KSEPVKNLDEVLNNVELQP-------------------LKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISEC---------------QRLT--VDPTKIYYPPTYLQIP 2122
            GG  S S ++  KR + R  SLTSL I+SHHP F  F+ CL ILK++I+  +E    +  G      +D++W  L G   + T S++ HDV EIETWI RLLS+PVPV G TR+E+ +L  T+ +P++FALPD+ RF LVDFPLH   EL+GVE  LK++  ++ ENKV+ QS++YN LS S++A   +LYPLEYMFP+  LLPT +  AEQL  AP+PF+ G+P+SFL+ +K+F++P D+W  DLD        + K+ P T    ++   P  +  IL  H+KQAL    A N          S+ D  +  P+  + +V   ++  P                    +PS       NP ++G D DSVDV+TRVAMVRFFN+Q+ L N+ + TR L+LYPRP+VAFQ+++F++SR   S F+ + ++TQAV+F  EW++ P N  F ++ +   DP  +GDK KWF  +L PI F V+ +  S    L   +    +   PTDESGSDS+    SSSS   L++  S++A S +S                 Q L+  +DP  +Y+PP+ LQ P
Sbjct:  238 GGSHSPSPRASRKRTKLRNQSLTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVSDATPSIVLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPTPFVIGVPASFLVYKKNFRLPDDIWVVDLD--------STKLTPPTGGYEEIPPLPEPEGTILKNHLKQALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALTPIRFSVWDDGSSLNGALRSLKQLECQ---PTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPSLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYP 804          

HSP 2 Score: 228.409 bits (581), Expect = 1.550e-58
Identity = 119/314 (37.90%), Postives = 183/314 (58.28%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIE-PD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            R+YLF+ L GK DR  +W+ M FWED F DAV QER++IG+DQ   ++M+RY  L  +E++RLE +EDRLL  +L N  A ++M+ V   +I+ K R ++GKSHI L  S  ++ ++D I  L GND+DL    SR    Q F V+ G D  G +   E+  D ++LRS+ G I  RWW ER +  T   K  ++C+ RR   +  + + Y +K KEL   I++  E+  +    P+   +F V D  T   G L++   G+     N+ +FV   ++R+CFT+K   F++E  +P T+NL  R ++S  ++ I
Sbjct: 1452 RVYLFEGLLGK-DRLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGTPTNVPELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNSKFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMSDQI 1764          

HSP 3 Score: 123.635 bits (309), Expect = 1.379e-26
Identity = 57/109 (52.29%), Postives = 75/109 (68.81%), Query Frame = 2
Query:  179 SICPRLIDYLSIVGLRN--PNRSGHKN-----SVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSR--NAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER 478
            S+CPRL+DY++IVG     P   G +        VP+LLRRYP +DH +  LP D+VYFCQP+GC S    R  +A +D  +FVF LTDK++G+ RYGIC+NF+RPIER
Sbjct:    8 SLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIER 116          

HSP 4 Score: 73.1738 bits (178), Expect = 2.630e-11
Identity = 44/164 (26.83%), Postives = 87/164 (53.05%), Query Frame = 2
Query: 2519 KSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKK 3010
            KS   + P   RK ++ER+ LI   K +     +   +    +++   + ENQ  ++ VT+++L G     +   R K ++E+E+YR L+LS++NK L+K I   + +I+DV + ++  +  ++   + +  G++ ++      G  SVF  +E+  THY  K+
Sbjct: 1139 KSGINFDPFPGRKGLVERTPLI---KHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKKI-APDDHIDDVCV-TKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKE 1297          
BLAST of MAP kinase-activating death domain protein vs. TrEMBL
Match: R7VC38 (UDENN domain-containing protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_182066 PE=4 SV=1)

HSP 1 Score: 564.688 bits (1454), Expect = 2.451e-176
Identity = 316/690 (45.80%), Postives = 448/690 (64.93%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVV--PELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQG-------GRESMSRQSKR-----IRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDER-IKRRSNG-----KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALK--VCQA------LNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESD-CPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGIS-------ECQRLTVDPTKIYYPPTYLQIPSINIIESE 2146
            CPRL+DY+ IVG RNP+R+   NSV   PELLRRYP  DHK+ AL  DVV+FCQP+GCI+    R + ++  +FVF+LT+K+TGR RYGICLNF+R  E+R      +       ++S++  S   G       G  + S ++KR      R R ++LTSL ++SHHP F  F+ CL +L+++I+  +ER   RR  G     +D++W  + G   ++T S++ H+V EIETWI RLLS+PVPV G T++E+++LP+ L +PV+F+LPD+ RF LVDFPLH   EL+GV+  +K+   ++LE+K+ILQS++YN LS S++A   +LYPLEYMFP+  LLPT M SAEQL  AP+PFI G+P+SFL+ ++ F +P DVW  DLD        ++K++P +N D    LP P +  +L  H+KQ  K   C        L+ +    +P+KNL+ +  N ++   + S +   GFNPLI+GND DSVDV+TRVAMVRFFNS +IL N+T+ TR L+LYPRP+VAFQ+++F KSR +++ F  KL++ QAV+FF EW ++P N VF +I +  YDP LIGDK KWF   L+ I+FKVY E  S     +     +  SD  PTDESGSDSD    +SSS   L++   D+ +S I+       E Q LTVD ++++ PP+ LQ+P   I  S+
Sbjct:    6 CPRLLDYIVIVGSRNPSRN---NSVAQTPELLRRYPVEDHKDFALAPDVVFFCQPEGCINVGPKRMSLRESTSFVFSLTEKDTGRTRYGICLNFYRSFEKRPTYAANKAERHTSIASSASTSSGEHGVLHPPAPGDPNRSPRAKRKQKVQGRVRNNTLTSLCLISHHPFFSTFRECLFVLRKLIDSCNERSCSRRIGGSRAGTRDTVWGVITGQHSDSTPSLVMHEVREIETWILRLLSAPVPVPGRTKVEITVLPKELHQPVMFSLPDHTRFSLVDFPLHLPLELLGVDLCMKVLTLIMLEHKLILQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCMSSAEQLLLAPTPFIIGVPTSFLMYKRGFMLPDDVWLIDLD--------SNKILPPSNADELPPLPEP-EGTVLKNHLKQVGKSLYCHTSMLLLYLSDVHAGPQPIKNLETIAQNPDVWKRRSSFTSTQGFNPLIYGNDVDSVDVATRVAMVRFFNSPNILANFTEHTRTLRLYPRPVVAFQLNSFFKSRPIKTVFTAKLARAQAVEFFAEWALSPANVVFQRIQTGVYDPLLIGDKGKWFSHGLQKIDFKVYDEATSTLGPAIATAVESRGSDENPTDESGSDSDGAESTSSSYSSLSDFVIDIQNSEINAFVEIYPEDQVLTVDESRVFSPPSALQVPDTPISNSD 683          
BLAST of MAP kinase-activating death domain protein vs. TrEMBL
Match: A0A2T7PJ46 (UDENN domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_04707 PE=4 SV=1)

HSP 1 Score: 572.392 bits (1474), Expect = 1.272e-171
Identity = 331/769 (43.04%), Postives = 466/769 (60.60%), Query Frame = 2
Query:  188 PRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPI-----KVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDER--IKR----RSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPAD-----FNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGIS--------ECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNS 2422
            PRL+DYL I G R+PN + H  S  PELLRRYP  DHK+ ALP DV++FCQP+GCIS    R + ++  +FVFTLT+K++G VRYG+C+NF+RP E+R        KV R S     S  +   S     R   +R+  R+R  T  LTSL I+SHHP F  F+ CL IL+ +IE   +R  ++R    ++  +D++W  L G + EN ++++ H+V EIETWI RLLS+PVPV G TR+E+++LP+ L  P++FALPD+ RF LVDFPLH   EL+GV+  LK+  A++LE+KVILQS++YN L+ S++A  ++LYPLEYMFP+  LLPT M SAEQL  AP+PF+ G+P+SF   ++ F +P D+W  DLD                   N ++ PP AD            +K  LK  QAL S+S   +P+KNLD +  N E+   + S S  +GFNPLI+GND DSVDV+TRVAMVRFFNS +ILGN+T+ TR L+LYPRP+VAFQ+ +F+KSR  ++ F  +L++TQAV++F EW++ P N V+ ++ +  YDP LIGDK KW+ Q L+P+ F VY +N S     + +       D PTDESGSDS+    +SSS   L++  +++ +S I         E Q L VD  K++ PP  L +P      S  DS F ++ +S+     +S                 H++  K        S++ K F     YD+ N T T+ +   + +T L GS  S  S
Sbjct:    9 PRLLDYLVIAGSRHPNSNNHV-SQTPELLRRYPLEDHKDFALPNDVIFFCQPEGCISVGPKRMSLRETTSFVFTLTEKDSGIVRYGVCVNFYRPFEKRMHSSECWQKVDRSSTGSTHSVETLSSSECDRPRSPRARRKSRVRNNT--LTSLCIISHHPFFSTFRECLFILRRLIEACHDRSCVRRVGGSKATARDTVWGVLTGQTSENISTLVMHEVREIETWILRLLSAPVPVPGKTRVEVNVLPKELQAPLLFALPDHTRFSLVDFPLHLPLELLGVDTCLKVLTAIMLEHKVILQSRDYNALTMSVMAFVSMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPFLIGVPASFFRYKRDFPLPDDIWIVDLDA------------------NKITSPPNADPLPPLPEPECTGLKTHLK--QALASMSMTPQPIKNLDAIAQNPEMWQRRESFSSQTGFNPLIYGNDVDSVDVATRVAMVRFFNSPNILGNFTEHTRTLRLYPRPVVAFQLYSFMKSRPQRTHFTARLARTQAVEYFAEWSLCPNNLVYQRVQTAVYDPSLIGDKPKWYAQHLQPVEFHVY-DNSSSLAAAITSVSETLSDDIPTDESGSDSEGAESTSSSYSSLSDLIAEMTNSEIDGETPSFVPENQILAVDEAKVFCPPDKLHVPEAPSTNS--DSAFSLQEDSDMSSSESSSAASS---------PEPHDVQTK-------DSEEHKIFR----YDSGNITETTYSLSSMSRTDLPGSPHSPRS 731          

HSP 2 Score: 233.802 bits (595), Expect = 5.787e-58
Identity = 117/280 (41.79%), Postives = 173/280 (61.79%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRS-----VIEPD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFV 4552
            + YLF+ L  K DRS++WD M FWED+F DAV QER+IIG+DQ   ++M+RYN L   EK+RLE++EDRLL ++L N + FM+MMRV   ++K K R ++GKSHI L  S ++N L+D I +L+GND+DL    SRQ   Q F V+ G D  GD+   E+  DC+LLR+++G I+ RWW E+ +  T   K  V+C+ R+   +V ++Q Y KK +EL  ++++  EK  S     +  P+   +F V D  +   G L++   G+     N+     FV
Sbjct: 1259 KTYLFEGLIAK-DRSRMWDQMQFWEDMFLDAVAQERDIIGMDQRPSEMMERYNSLGDTEKKRLEQDEDRLLSVMLYNLVGFMLMMRVPKAEVKKKVRRLLGKSHIGLHHSQDVNNLLDVITHLQGNDIDLKPMGSRQMQKQSFTVHWGTDNTGDMLFMEVCDDCLLLRNVTGMIHDRWWFEKLVNMTYCPKTKVLCLWRKNGEKVQLNQFYTKKCRELYFSVKEAMEKAASRTTGRIPGPELGGEFPVQDLKSGQGGLLQVCMEGIGLLLANSKASTCFV 1537          

HSP 3 Score: 80.8777 bits (198), Expect = 2.946e-11
Identity = 45/158 (28.48%), Postives = 87/158 (55.06%), Query Frame = 2
Query: 2525 NPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHY 2998
            N  + P+  RK ++E+S L+   K A +  +R        + + S + ENQ+ +++V + +L G   + +   RIK ++E+E YRN ++SR+N+NL+K +     +I DV + S   F  ++ + + +++G+E +       G  S ++ LE+  THY
Sbjct:  855 NKPFTPLGNRKALVEKSGLV---KHATNIKQRDQPKPTAAEPRGSTNSENQQFLKEVINGVLDGQGVSWLKISRIKKLMEDENYRNFVVSRLNRNLDKKVPEDARHIEDVCL-SRPVFKGMLGMCKAIISGLEHTIQNHGLGGMASAYMVLEIAHTHY 1008          
BLAST of MAP kinase-activating death domain protein vs. TrEMBL
Match: A0A210Q284 (MAP kinase-activating death domain protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT17866 PE=4 SV=1)

HSP 1 Score: 572.007 bits (1473), Expect = 1.036e-170
Identity = 305/684 (44.59%), Postives = 440/684 (64.33%), Query Frame = 2
Query:  182 ICPRLIDYLSIVGLRNPNRSGHKNSVV--PELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQG-GRESMSRQSKRI----RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDER--IKR----RSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGIS--------ECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIK 2170
            +CPRL+DY+ +VG R+PN   H N+V   PELLRRYP  DH +  LP D+++FCQP+GCIS    R + ++  +FVFTLT+K++G VRYGIC+NF+RPIE++   K          +  +      +  GR+   R S+R+    R R ++LTSL IVSHHP F  F+ C+ IL+++I+   +R   KR    R + +D++W  L G   +N   ++ H+V EIETWI RLLS+PVPV G TR+E+ +LP+ L  P++FALPD+ RF L+DFPLH   EL+GV+  LK+  A++LE KV++QS++YN LS S++A   +LYP+EYMFP+  LLPT M SAEQL  AP+P+I G+P+SFL  +  FK+P DVW  DLD  ++       + P          P P +  +L  H++QAL       S+S   +P+KNLD +  N +    + S S  +GFNPLI+GND DSVDV+TRVAM+RFFNS + LGN+T+ TR L+LYPRP+VAFQ+ +F+KSR  ++ F  +L+++QAV+++ EW++ PKN VF ++ +  +DP LIGDK KW+   L+ + FKVY +N S    +  + D  ++ D PTDESGSDSD    +SSS   L++  +D+ +S I         E Q L+VD +K+Y PP  L +P   +  S  DS F ++
Sbjct:    9 LCPRLVDYIIVVGSRHPN---HNNNVAQTPELLRRYPLEDHHDFPLPPDIIFFCQPEGCISVGPKRMSLRETTSFVFTLTEKDSGLVRYGICVNFFRPIEKKYSEKSKSERFYGGNADGNTLGIGIESDGRQRSPRTSRRLKTAGRVRNNTLTSLCIVSHHPFFSTFRECMFILRKLIDSCHDRSCTKRIGGSRGSSRDTVWGVLTGQGTDNITGLVMHEVKEIETWILRLLSAPVPVPGKTRVEVQVLPKDLQPPLLFALPDHTRFSLIDFPLHLPLELLGVDTCLKVLAAIMLEYKVVMQSRDYNALSMSVMAFVTMLYPMEYMFPVIPLLPTCMSSAEQLLLAPTPYIIGVPASFLPYKNGFKVPDDVWLVDLDSNKMTMGKLEDIPP---------FPEP-EGTMLKNHLRQALA------SMSMTPQPIKNLDALAQNPDTMKRRESFSSQTGFNPLIYGNDVDSVDVATRVAMIRFFNSANTLGNFTEHTRTLRLYPRPVVAFQMYSFLKSRPARTHFTARLARSQAVEYYAEWSLCPKNVVFLRVQTSVFDPLLIGDKPKWYAHQLQSLEFKVYDDNSSLAAAVTSSMDLISD-DNPTDESGSDSDGAESTSSSYSSLSDFVTDMVNSEIDGDTLCFVPENQVLSVDESKVYTPPNKLHLPDTPVTTS--DSAFSMQ 670          

HSP 2 Score: 244.202 bits (622), Expect = 4.086e-61
Identity = 126/333 (37.84%), Postives = 196/333 (58.86%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRS-----VIEPD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSAN----LIFRLFQSQVQKF 4699
            RMYL++ L  K DRS++WD M FWEDV+ DAV QER+IIG+DQ   ++M+RYN L  +EK+RLE++EDRLL  +L N  +FM+MMR +  +++ K R ++GKSHI L  S +++ ++D    + GN++ L    SR    Q F V+ G D  GD+   E+  DC++LRS+ GAI  RWW E+ +  T   K  V+C+ R+   +V ++Q Y KK +EL   +++  EK  +     +  P+   +F V D  +   G L++   G+     N+  F++   +++CFT+K   F++E  D   + +T   FKS  AN    +I R+F  Q  K 
Sbjct: 1277 RMYLYEGLVTK-DRSRLWDQMQFWEDVYLDAVAQERDIIGMDQGPGEMMERYNSLGDSEKKRLEQDEDRLLSTMLYNLASFMVMMRCNKQELRKKVRRLLGKSHIGLVYSQDVHTVLDLAMTMHGNEISLKPMGSRFMQKQSFTVHWGSDNSGDMLFMEVCDDCIILRSVMGAICDRWWYEKLVNMTYCPKTKVLCLWRKHGEKVQLNQFYTKKCRELYFCVKEAMEKAAARNNGKIAGPELGGEFPVQDLKSGQGGLLQVCMEGIGLLLANSKSFIELGRIKKCFTQKGGIFVLEEFDTKNRQVTLHKFKSRMANPIALMIHRIFAVQHAKM 1608          

HSP 3 Score: 82.0333 bits (201), Expect = 1.687e-11
Identity = 66/261 (25.29%), Postives = 128/261 (49.04%), Query Frame = 2
Query: 2534 YKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSI-DYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISS----KNDINATHGV 3301
            + P+  RK ++E+S L+   K A    ++ ST      D +   + ENQ+ ++ +   +L G   + +   RI+ ++E+E YRN ++SR+NKNL+K +N    ++ D  + ++  F  ++ + + +V+G+E +Y      G  S F+ LE+  THY  K+ +      S+ + + VT +   PY           S++   + + Q + +  L +  +  +        G P+ +NGE +E IEI S    +  ++A H  
Sbjct:  875 FAPLGNRKALVEKSGLV---KHATPRKQKDSTKQTSSSDSRGASNSENQQFLKDIVTNVLDGQGVSWLKLGRIRRLMEDENYRNFMVSRLNKNLDKKLNDDTQHLEDASM-TKQVFKGMMTLMKAIVHGLEHTYQNHGIGGMASAFMVLEIAHTHYWAKE-IIQGTGSSNRSDTSVTPEINSPY-------GSHESLSSRSEGSPQKSESHSLHEEHMVAAL-------GSPVLVNGEEFENIEIQSMSLHQGSVDAGHAT 1116          
BLAST of MAP kinase-activating death domain protein vs. TrEMBL
Match: V4ALC2 (UDENN domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_103875 PE=4 SV=1)

HSP 1 Score: 563.148 bits (1450), Expect = 2.656e-168
Identity = 331/751 (44.07%), Postives = 469/751 (62.45%), Query Frame = 2
Query:  185 CPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRS-PIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRP--------RTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDER-IKRRSNG-----KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGIS---------ECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEM-NIKKREREVKRQHNLSNKRKSVDKNQSKDPK-------TFTTGNAYDT 2341
            CPRL+DY+ I G RN   + +  +  PELLRRYP  DHK+  LP DV++FCQP+GCIS    R + ++  +FVFTLT+K++G VRYGIC+NF+RP E+RS    V   + ER  S +S          ES   +S R R         R ++LTSL IVSHHP F  F+ CL IL+ +I+   +R   RR  G     +D++W  L G   +N  S++ HDV EIETWI RLLS+PVPV G TR+E+ +LP+ L  P++FALPDN RF L+DFPLH   EL+GV+  LK+  AV+LE+KVILQS++YN LS S++A   +LYPLEYMFP+  LLPT M SAEQL +AP+P++ G+P+SF L +K F +P D+W  DLD        ++K+ P +  +   SLP P +  +L  H+KQAL       S+S   +P+KNLD +  N E+   + S S  +GFNPLI+GND DSVDV+TRVAM+RFFNS +ILGN+ + TR L+LYPRP+VAFQ+ +F+KSR+V++ F  +L++TQAV+++ EW+++P+N V+ ++ +  YDP LIGDK KW+   L+ + F VY  N S +  L    D  ++ + PTDESGSDS+    +SSS   L++  +D+ +S I          E Q L VD +K++ PP  L +P +N   S  DS   +  +S+     +S      NI + ERE K   ++     +V    S+ P+       T +T +A+D 
Sbjct:   10 CPRLLDYIVITGSRN-PNNNNNVAQTPELLRRYPLEDHKDFPLPTDVIFFCQPEGCISVGPKRMSLRETTSFVFTLTEKDSGIVRYGICVNFFRPFEKRSHTTSVDWQNKERNSSLASTHSVDTVSSTESDKPKSPRARRKGGPSCRVRNNTLTSLCIVSHHPFFSTFRECLFILRRLIDACHDRSCVRRIGGSKGATRDTVWGVLTGQCSDNITSLVMHDVREIETWILRLLSAPVPVPGKTRVEVHILPKDLQAPLLFALPDNTRFTLIDFPLHLPLELLGVDTCLKVLTAVMLEHKVILQSRDYNALSMSVMAFVYMLYPLEYMFPVIPLLPTCMSSAEQLLSAPTPYLIGVPASFFLYKKGFLLPEDIWIVDLD--------SNKIHPPSKAEPLPSLPDP-EGAVLKTHLKQALA------SMSMTPQPIKNLDALAQNAEIWQRRESFSSQTGFNPLIYGNDVDSVDVATRVAMIRFFNSSNILGNFCEHTRTLRLYPRPVVAFQLYSFLKSRQVRTHFSVRLARTQAVEYYAEWSLSPQNLVYLRVHTGVYDPSLIGDKPKWYAHQLQSVEFHVYDPNSSLSAALNSVSDVLSD-EIPTDESGSDSEGAESTSSSYSSLSDFVTDMVNSEIDGETPAAFIPENQILAVDESKVFTPPDKLHVPDVNATNS--DSALSLPEDSDVSSSASSSPTYSDNIPRSEREDKDLEDIKILLGAVLPGPSEAPRSPKLPSFTKSTNSAFDV 741          

HSP 2 Score: 254.218 bits (648), Expect = 2.924e-64
Identity = 127/314 (40.45%), Postives = 192/314 (61.15%), Query Frame = 2
Query: 3743 YLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRS-----VIEPD-TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSAN 4660
            +LF+ L GK +RS++WD M FWEDVF DAV QER+IIG+DQ   ++MDRY  L   E++RLE +ED++L  +L N ++FM+M+RV+  +IK K+R ++GKSHI L  S  +N L+D I  L GND+DL    SRQ   Q F V+ G D  GD+   E+  DC+LLRS++G+I+ RWW E+ +  T   K  V+C+ R+   +V ++Q Y KK KEL   +++  EK  +     +  P+   +F V D  +   G L++   G+     N+ +F++  ++R+CFT K   F++E  D   K +    FKS  A+
Sbjct: 1194 FLFEGLLGK-ERSRLWDQMQFWEDVFLDAVAQERDIIGMDQSPSEMMDRYASLGDMERKRLEHDEDKILATILFNLVSFMVMLRVNKTEIKKKSRRLLGKSHIGLIHSQEVNNLLDQITMLHGNDIDLKPICSRQMQKQSFTVHWGTDNTGDMLFMEVCDDCLLLRSVTGSIHDRWWFEKLVNMTYCPKTKVLCLWRKNGEKVQLNQFYTKKCKELYFCVKEAMEKAAARNSGKLPGPELGGEFPVQDLKSGQGGLLQVCMEGIGLLLANSKFFIELAHIRKCFTLKGGIFVLEEFDTKNKKIIQHKFKSRMAD 1506          

HSP 3 Score: 75.485 bits (184), Expect = 1.389e-9
Identity = 34/116 (29.31%), Postives = 71/116 (61.21%), Query Frame = 2
Query: 2651 KKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHY 2998
            + S + ENQ+ ++ V + +++G   + +   R+K ++E+E YRN ++SR+NKNL+K +  +  +I+DV + S   F  ++ + + +++G+E ++      G  S ++ LE+  THY
Sbjct:  884 RSSSNSENQQFLKDVVNNVMEGQGTSWLKMGRVKTLMEDENYRNFVVSRLNKNLDKKVTDESLHIDDVCV-SRQVFKGMLAMLKAIIHGLEKTFQNHGLGGTASAYMVLEIAHTHY 998          
BLAST of MAP kinase-activating death domain protein vs. TrEMBL
Match: A0A433TIA0 (UDENN domain-containing protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_010917 PE=4 SV=1)

HSP 1 Score: 550.436 bits (1417), Expect = 9.072e-168
Identity = 311/686 (45.34%), Postives = 424/686 (61.81%), Query Frame = 2
Query:  170 ANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPI-------KVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDER-IKRRSNG-----KDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLL--KHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTL-----------HLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGI--------SECQRLTVDPTKIYYPPTYLQIPS 2125
            +N   CPRL+DYL I G R PN   ++ S  PELLRRYP  DHK+  LP DV++FCQP+GCIS    R + ++  +FVFTLT+K++G VRYGIC+NF+RP E+R+ I       KV + +     S  +   S     R S SR  K+ R R ++LTSL IVSHHP F  F+ CL IL+ +IE   +R   RR  G     +D++W  L G + +N +S++ H+V EIETWI RLLS+PVPV G TR+E+ +LP+ L  P++FALPDN RF LVDFPLH   EL+GV+  L+   A++LE+KVILQS++YN LS S++A  ++LYPLEYMFP+  LLPT M SAEQL  AP+P++ G+P+SF   +K F +P D+W  DLD  ++L  K              DL             H+KQAL       S+S   +P+KNLD +  N +L   + S + ++  NPLI+GND DSVDV+TRVAMV+F NS + LGN+T+ TR L+LYPRP+VAFQ+ +F+KSR  ++ F  KL++TQAV+F  EW++ P N V+ ++ +  YDP LIGDK KW+ Q L+ ++F  Y  + S                   Q  +T  D PTDESGSDSD    +SSS   L++  SDL +S I         E Q L VD   +Y PP+ L +P 
Sbjct:    5 SNRYFCPRLLDYLVIAGCRFPN-GNNQMSQTPELLRRYPLEDHKDFPLPNDVIFFCQPEGCISVGPRRMSLRETTSFVFTLTEKDSGLVRYGICINFYRPFEKRAHIPSAEAWQKVDKSATGSSHSVETLSSSECDRPR-SPSRTRKKSRVRNNTLTSLCIVSHHPFFSTFRECLFILRRLIEACHDRSCVRRVGGSKATVRDTVWGVLTGQTSDNISSLVMHEVKEIETWILRLLSAPVPVPGKTRVEVHVLPKELQAPLMFALPDNTRFSLVDFPLHLPLELLGVDTCLRALTAIMLEHKVILQSRDYNALSMSVMAFVSMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYLIGVPASFFGYKKGFPLPDDIWVVDLDANKILPPKGAEPLPPLPEPEGTDLK-----------THLKQALA------SMSMTPQPIKNLDALAQNPDLWQRRDSLT-MTQRNPLIYGNDVDSVDVATRVAMVKFLNSPNTLGNFTEHTRTLRLYPRPVVAFQLYSFLKSRAERTAFTAKLARTQAVEFLSEWSLCPTNLVYQRVQTNVYDPSLIGDKPKWYAQQLQLVDFHAYDRDSSLGAAVASVVSAAQERAAGGQGGHTSDDGPTDESGSDSDGAESTSSSYSSLSDFVSDLYNSDIRGETPSFQREMQILAVDEAAVYSPPSQLHLPG 670          

HSP 2 Score: 74.7146 bits (182), Expect = 2.323e-9
Identity = 36/152 (23.68%), Postives = 86/152 (56.58%), Query Frame = 2
Query: 2543 ISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHY 2998
            +  RK ++ERS L+     A +  ++      + + + + + ENQ+ +++V   +L+G   + + + R+  ++E+E YRN ++SR+N+N+++ +     ++ DVM+ S++    ++ + + +++G+  + N     G  S ++ LE+  THY
Sbjct:  866 LGNRKALVERSGLVRHSSTAKTPQQKEPVK--VSESRTNANSENQQFLKEVISSVLEGKGMSWMKEGRVNKLMEDENYRNFVVSRLNRNMDEKVPEDAVHVEDVMV-SKNVLKGMLKLIKSIIHGLGHTINNHGLGGMASAYMVLEIAHTHY 1014          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Cavefish
Match: madd (MAP-kinase activating death domain [Source:ZFIN;Acc:ZDB-GENE-030131-8076])

HSP 1 Score: 469.544 bits (1207), Expect = 2.726e-138
Identity = 246/575 (42.78%), Postives = 355/575 (61.74%), Query Frame = 2
Query:  260 VPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSP-----------------IKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRI-RPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNG-----KDSIWPTLLGS-SVENT--NSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYREN 1894
             P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D ++FVF LTDK++G  RYGIC+NF+R  +R  P                 + ++  S     +  +       GG+    ++S+   R R  +LTSL ++SH+P F  F+ CL ILK +++    R+ +R        +D++W    G+ SVE     S +  D+ EIE+W++RLL SPVPVAG+ R+++ +LP  L   + FALPD+ RF +VDFPLH   EL+GV+  LK+   +LLE+KV+LQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FKMP DVW  DLD  +++  +N +++P              + + L KH+KQAL       S+S  ++P+ N ++     EL  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  ++ F  KLS TQAV+++ EW + P N  F +I +  YDP LIGDK KW+   L+P+ ++VY  N
Sbjct:   26 TPQLLRRYPLEDHPDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDSSFVFALTDKDSGITRYGICVNFYRSFQRDKPPQMDPAVEATEKSDPSTLSLSGDSTAPPAADGAVPPGEGGGGKSPRPKRSRAAARNRNSTLTSLCMLSHYPFFSTFRECLYILKRMVDCCSHRLNQRPGAAKGTQRDAMWRVFTGALSVEEKEKGSQLLQDLKEIESWVYRLLRSPVPVAGLRRVDVEVLPHELQPALTFALPDSSRFCMVDFPLHLPLELLGVDACLKVLSCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDCNKVIAPSNAELLPPLPE---------PESSELKKHLKQALA------SMSLNTQPILNFEKFQEGQELPLLPPGRDKSSPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQATSFLASRPRRTGFADKLSHTQAVEYYGEWALNPTNLAFQRIHNNVYDPSLIGDKPKWYAHQLQPVFYRVYDGN 585          

HSP 2 Score: 202.986 bits (515), Expect = 6.382e-52
Identity = 108/296 (36.49%), Postives = 171/296 (57.77%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVE 4600
            R+YL + L GK +RS +WD + FWED F DAV  ERE +G+DQ  ++++DRY  L  ++++RLE +EDRLL  +L+N IA+M+M +V    I+ K R ++GKSHI L  S  IN+++D +  L G ++ +  + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E
Sbjct: 1213 RIYLCEGLLGK-ERSTLWDQVQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGDHDRKRLEDDEDRLLATLLHNMIAYMLMTKVSKNDIRKKVRRLMGKSHIGLTYSQEINEVLDRLPQLTGRELPIRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELNHIKKCNTVK-GVFVLE 1506          

HSP 3 Score: 62.3882 bits (150), Expect = 5.745e-9
Identity = 31/113 (27.43%), Postives = 62/113 (54.87%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L+G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV I +   +  ++ + +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  909 ENQQFLKEVVQSVLEGQGVGWLNMKKVRRLLENEQLRIFVLSKLNRAVQSEEDARQEVIRDVEI-NRKVYKGMLDLLKCTVSSLEHSYTNAGLGGMASVFSLLEIARTHYQTK 1020          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Cavefish
Match: sbf1 (SET binding factor 1 [Source:ZFIN;Acc:ZDB-GENE-040718-139])

HSP 1 Score: 85.5001 bits (210), Expect = 4.817e-16
Identity = 119/504 (23.61%), Postives = 194/504 (38.49%), Query Frame = 2
Query:  269 LLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPI------ERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEI--ETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRV-QSDFVKKLSKTQAVQFF 1753
            +L+R+P  D ++   PQ +  FCQP G       R  A   + FV  LTD  + R  Y  C  FW  +      ER +    T V                      +S+  +   P+     SL++VS     + F+ CL ++                                    +H D + +  ET I  LL+  +P+AG ++  ++L         V   P ND  P+    +  +F  +G+   L LF A L E+K++  SK+Y  L+ +  AL A+++PL+Y F    +LP  +    ++ + P+PFI G+ S F   R   +   DV   DLD            +P+  +   L LP P     L++H + AL +                LD  L  V  Q   PS ++ S              D   R   +  F    +   Y     I++++P P++ F    F+  R + + DF+ K+    A   F
Sbjct:   25 ILQRFPEKDWEDNPFPQGIELFCQPSGW-QLVPERLPA---SFFVAVLTDINSER-HYCACFTFWESVENPQVRERETDEDETLV----------------------LSQPEQLYAPK-----SLVLVSRLDHTEVFRNCLGLIY----------------------------------TVHVDSLNVPLETVIGNLLTCVIPIAGGSQRTITLGAGDRQ---VIQTPINDSLPVSGCSVAQLFRQLGIVNVLYLFCAALTEHKILFLSKSYQRLTDACRALLAIMFPLKYSFTYVPILPGKLL---EVLSTPTPFIIGVNSFF---RSETQELLDVIIADLD-------GGTVTIPEC-VHISL-LPEP-----LLQHTQTALSMV---------------LDPEL-EVADQAFPPSSTQPSALK---------IQDKEIRAVFLWLF--AQLFQGYRWCLHIIRIHPEPVIRFHKAAFLGQRALSEDDFLMKVLDGMAFAGF 412          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Cavefish
Match: dennd4b (DENN domain containing 4B [Source:NCBI gene;Acc:103038730])

HSP 1 Score: 80.4925 bits (197), Expect = 1.523e-14
Identity = 122/514 (23.74%), Postives = 198/514 (38.52%), Query Frame = 2
Query:  251 NSVVPE--LLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKD---FNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVA-----FQVDNFIKSR-RVQSDFVKKLSKTQAVQFFCE 1759
            N++V E  L+ RYP  D ++  LP  V  FC P G        N       F+TFV T     +G   YG  + F+    R +  +  RV L  +              R  ++ ++ +++       S+ ++SH P F  FQ           KF   + R S     + P      +E   S   H+V             P P     RI + L P      ++   P +   PL       + + +G E A  L +AVL E+K++L S   +VL++   AL ++ +PL +  P   L P  +     +  AP PFI G+ SS+  +   +  PSDV   DLD   + +  + K +   ++       P     IL   +    K    L  I    +    L+ +L + +                LI+G     +++  + A +RF +   +L  Y  F   +   P          F +  F+KSR R Q  F  +L++TQ    F E
Sbjct:  199 NALVYEAGLISRYPEEDLESFPLPDSVPVFCLPMGVTVESWPLNTKYQLPVFSTFVLT---SASGDKVYGAAIQFYEAYPREALSERQRVRLGLVSVVD----------RRPITNRTVQVK------KSVCVLSHWPFFTVFQ-----------KFLTFVYRYSISGPHVLP------IEKHISSFMHNV-------------PFPSPQRPRILVQLSP---YDNLLLCQPVSSPLPLSGASFVKLLQNLGAENACTLLLAVLTEHKLLLHSLRPDVLTSVSEALVSMTFPLRWPCPYIPLCPLRL---ADVLCAPMPFIVGVHSSYFDL---YDPPSDVVCVDLDTNTIFQSEDKKPLSWRSL-------PRKHGKILFSSLTSLHKT---LEKICTPGQEEATLEFLLTDYD----------------LIYGRQKQ-LELEIQEAFLRFMS--CLLRGYRSFLLPITQAPSDRTTDCSSLFNLQGFLKSRDRTQQKFYTQLTRTQMFTQFIE 625          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Cavefish
Match: dennd4b (DENN domain containing 4B [Source:NCBI gene;Acc:103038730])

HSP 1 Score: 80.1073 bits (196), Expect = 1.863e-14
Identity = 122/514 (23.74%), Postives = 198/514 (38.52%), Query Frame = 2
Query:  251 NSVVPE--LLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKD---FNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVA-----FQVDNFIKSR-RVQSDFVKKLSKTQAVQFFCE 1759
            N++V E  L+ RYP  D ++  LP  V  FC P G        N       F+TFV T     +G   YG  + F+    R +  +  RV L  +              R  ++ ++ +++       S+ ++SH P F  FQ           KF   + R S     + P      +E   S   H+V             P P     RI + L P      ++   P +   PL       + + +G E A  L +AVL E+K++L S   +VL++   AL ++ +PL +  P   L P  +     +  AP PFI G+ SS+  +   +  PSDV   DLD   + +  + K +   ++       P     IL   +    K    L  I    +    L+ +L + +                LI+G     +++  + A +RF +   +L  Y  F   +   P          F +  F+KSR R Q  F  +L++TQ    F E
Sbjct:  199 NALVYEAGLISRYPEEDLESFPLPDSVPVFCLPMGVTVESWPLNTKYQLPVFSTFVLT---SASGDKVYGAAIQFYEAYPREALSERQRVRLGLVSVVD----------RRPITNRTVQVK------KSVCVLSHWPFFTVFQ-----------KFLTFVYRYSISGPHVLP------IEKHISSFMHNV-------------PFPSPQRPRILVQLSP---YDNLLLCQPVSSPLPLSGASFVKLLQNLGAENACTLLLAVLTEHKLLLHSLRPDVLTSVSEALVSMTFPLRWPCPYIPLCPLRL---ADVLCAPMPFIVGVHSSYFDL---YDPPSDVVCVDLDTNTIFQSEDKKPLSWRSL-------PRKHGKILFSSLTSLHKT---LEKICTPGQEEATLEFLLTDYD----------------LIYGRQKQ-LELEIQEAFLRFMS--CLLRGYRSFLLPITQAPSDRTTDCSSLFNLQGFLKSRDRTQQKFYTQLTRTQMFTQFIE 625          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Cavefish
Match: sbf2 (SET binding factor 2 [Source:ZFIN;Acc:ZDB-GENE-030911-10])

HSP 1 Score: 78.5666 bits (192), Expect = 6.827e-14
Identity = 50/154 (32.47%), Postives = 82/154 (53.25%), Query Frame = 2
Query:  812 IETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLD 1273
            +E  +  LL+  VPVAG ++   SL         +   P ND  P+    +  +F+ +G++  L LF AVL E+KV+  S +Y  L  +  AL+AL++PL+Y +P   +LP+ +    ++  +P+PFI G+ S F   ++      DV   DLD
Sbjct:   85 LEGLVASLLTCMVPVAGGSQKLFSLGAGDRQ---LIQTPLNDNLPVTTKSVALLFQQLGIQNVLSLFCAVLTEHKVLFHSTSYQRLEEACRALEALMFPLKYSYPYIPILPSRLL---EMLNSPTPFIMGVHSMF---QREIHELLDVIIADLD 229          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Sea Lamprey
Match: sbf1 (SET binding factor 1 [Source:ZFIN;Acc:ZDB-GENE-040718-139])

HSP 1 Score: 96.6709 bits (239), Expect = 4.734e-20
Identity = 119/511 (23.29%), Postives = 207/511 (40.51%), Query Frame = 2
Query:  266 ELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEI--ETWIFRLLSSPVPVAGITRIELSLLP--ETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRR-VQSDFVKKLSKTQAVQFFCEWNIAPKNT 1783
            ++++R+P  + ++   PQ +  FCQP G   +    N  K    FV  LTD E+ R  +  CL F+ P +    I       ++   +         G  E  S   +R  P+     SL++VS     + ++  L ++                                    IH D +++  E  +  LL+  +P +G  +   SL      +++P +     N+  P+    +  +F  +G+   L LF AVL E+K++  S +Y  L+ +  AL AL +PL+Y +    +LP    S  ++ + P+PFI G+ S+F   R  F+   DV   DLD           M+P+    +  +LP P      ++HI+ AL +             V   D  + ++   P  PS        P       +  D   R AM+R F    +L  Y    ++++++  P++ F    F+  R  V++DF+ KL  + A   F      P  T
Sbjct:   26 KIIQRFPDREWEDTPFPQGIELFCQPSGWKLS----NEWKQPTFFVAVLTDIESDR-HFCACLTFYEPEDSTYEIVTGHSFQQKDSES--------DGEGEDESHHMQRFAPK-----SLVLVSRLDYTEIYRQSLGLIY----------------------------------AIHVDDLKVTLENVVGNLLTCMIPTSGGAQKVFSLGAGDRQVIQPAL-----NNEIPVTGSSVILLFRQLGINNVLHLFCAVLTEHKILFHSSSYQRLTEACRALVALTFPLKYSYTYIPILPG---SLLEVLSTPTPFIIGVHSTF---RSEFQELLDVIIADLD-------GGTVMIPECVQVS--ALPEP-----YLQHIQTALSM-------------VLYPDLEVADMAFSPTSPSV-----IKP-------EMQDKEVRAAMLRLF--AQLLQGYRSCLQLIRIHSEPVIRFHKAAFLGQRGLVENDFIPKLLDSMAFAGFVSERGPPYRT 432          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010382.1 (pep scaffold:Pmarinus_7.0:GL482471:369374:413667:1 gene:ENSPMAG00000009395.1 transcript:ENSPMAT00000010382.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.4261 bits (215), Expect = 2.712e-17
Identity = 92/372 (24.73%), Postives = 149/372 (40.05%), Query Frame = 2
Query:  266 ELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEI--ETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILI 1375
            ++L+R+P  + ++   PQ +  FCQP G   T T R      + FV  LTD  + R  Y  CL F  P    +         E ++                + + ++   P+     SL++VS   C + F+ CL +L                                     H D + +  ET +  LLS  VP AG  +   SL         V   P ND  P+    +  +F  +G+   L LF A + E+KV+  S +Y  LS +  A+ AL++PL+Y +P   +LP  +    ++ + P+PFI G+ S F+       +P  V    L L   L  N   + P+   +  L +    D N+ +
Sbjct:    8 KILQRFPAKEWEDAPFPQGLELFCQPSG-WKTSTERVPP---SFFVVVLTDINSER-HYCACLTFQEPFLEPNDSDGEEEEQEEVDDT-------------GLVKPAQVFAPK-----SLVLVSRRDCTEIFRNCLGLLY----------------------------------TAHTDSLRVPLETLVGNLLSCVVPTAGGAQKLFSLGAGDRQ---VIQAPLNDTLPITGSSVAILFRQLGIRHVLNLFCAAVTEHKVLFHSSSYQRLSDACRAMLALMFPLKYSYPYIPILPAQL---LEVLSTPTPFIIGVHSMFI-----NHLPDLVRHTALVLELSLATNGLSVQPRVANEMHLEIATMYDINVTV 311          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Sea Lamprey
Match: dennd4a (DENN/MADD domain containing 4A [Source:ZFIN;Acc:ZDB-GENE-060503-285])

HSP 1 Score: 72.0182 bits (175), Expect = 1.209e-12
Identity = 125/532 (23.50%), Postives = 204/532 (38.35%), Query Frame = 2
Query:  269 LLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKD---FNTFVFTLTDKETGRVRYGICLNFWR--PIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDVMEIETWIFRLL-SSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVA-----FQVDNFIKSR-RVQSDFVKKLSKTQA-VQFFCEWNIAP-KNT--VFFQILSEKYD 1816
            L+ RYP  D+++  LP+ V  FC P G    C   N       F+TFV T +  E     YG  + F+   P ER +  + T++                  G  S            H+  S+ ++SH P F+ F   L  L  +                               SV   +++ IE  I   + ++P P     RI + L P      +  + P +   PL       +   +G E  L L  A L+ENK+++ S    + ++   AL ++++P  +  P   L P  + +   +  AP PFI G+ S +  +   +  P D+   DLD   + ++   K           +LP            K+  K+ QA        E  +N +E L     Q    S ++++G    +    + +++V  + A +RF    SIL  Y  + R +   P          F +  F+KSR R    F  +L+KTQ  ++F  E + A  KN    FF    EK D
Sbjct:  213 LISRYPVGDYESFQLPESVPLFCLPLGATIECWQANTKYPMPIFSTFVLTGSSAEK---VYGAAIQFFEAYPGERLTDRQKTQL------------------GMLSAVDHKPIAGKVAHASKSICLLSHWPFFEAFHKFLLFLYRL-------------------------------SVSGPNLIPIEKHISHFMHNTPFPSPQRPRILVQLSPH---DSLTLSQPASSPLPLSGASFSMLLVNLGPEHLLALLHAALMENKILIHSLRPAIQTSVAEALVSVIFPFHWQCPYVPLCPLALAT---VLNAPFPFIVGVDSRYFEL---YDPPLDIVSVDLDTNTIFQNEEKKGTSWK------ALP------------KKPCKILQA-GLTRLYQELQRNYNETLQQ---QRKDDSLTDVAG----VRCRSAHTLEVEIQEAFLRFM--ASILKGYRSYLRPITQAPSEKATDASSLFDLQGFLKSRDRSYHKFYTQLTKTQMFIRFIEECSFAGDKNASLAFFDDCVEKVD 655          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Sea Lamprey
Match: dennd5a (DENN/MADD domain containing 5A [Source:ZFIN;Acc:ZDB-GENE-071211-1])

HSP 1 Score: 71.2478 bits (173), Expect = 1.912e-12
Identity = 85/351 (24.22%), Postives = 143/351 (40.74%), Query Frame = 2
Query:  266 ELLRRYPHTDHKNGALPQDVV-YFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGSSVENTNSVIHHDV-------MEIETWIFRLLSS-PVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSD--VWFFDLDLRQL 1285
            ++L  YP+    N    QD V   C P G      +     +F++F+ T   +E G   YG  L F+  + R  PI+      E +++  +   +   G  ES S         T   +SLL + H   +   +  L   K I                 I P     +     + +H  V       + +E++++ LL   P+P  G + +  S      M PVV   P     PL D+ L  +FEL+G+E  ++LF   LLE++++L S++Y  L      +  LL+P  +      +LP    S      AP P++ G+     + R   ++P +  +   D+D R L
Sbjct:   24 KVLAHYPNNVEWN-LFDQDAVGMLCMPRGLSFRTQADRRDPEFHSFLIT---REDGSRTYGFALTFFEEV-RSEPIR------EAMQTLYAMHRAEQAGLAESTSSDFGDTDSATGLSSSLLRMRHSASYDSSRDTLFTSKCIC---------------LIAPMPFVQACRKALTQLHTAVTSPQPPPLPLESYVYNLLYEVPLPPLGRS-LRFS---SVYMPPVVCQRPGPHELPLFDYCLREVFELLGLENVVQLFTCALLEHQILLLSRDYQRLMVVAEGIMVLLFPFSWQHVYVPILPA---SLLHFLDAPVPYLMGLQIDGEMGRNGLELPQEASLCLVDVDNRSL 341          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Sea Lamprey
Match: dennd4a (DENN/MADD domain containing 4A [Source:ZFIN;Acc:ZDB-GENE-060503-285])

HSP 1 Score: 62.3882 bits (150), Expect = 1.043e-9
Identity = 76/319 (23.82%), Postives = 127/319 (39.81%), Query Frame = 2
Query:  845 PVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEI------SGFNPLIFGNDS--DSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVA-----FQVDNFIKSR-RVQSDFVKKLSKTQAVQFFCE 1759
            P P +   RI   L+  T    +V + P     P+      T+ + +G E A  L +  L ENK+++ S    VL+++  AL A+++P  +  P   L P    S   + +AP PFI G+ S +  +   ++ P DV   DLD   + +    K +               ++ +L K                   +P KNL   L N+  Q +  +   +      SG   L F   +    +++  + A +RF    SIL  Y  + R +   P          F +  F+KSR R  + F  ++ +TQ    F E
Sbjct:  358 PFPSSQRPRI---LVQATANDSLVLSQPIASPLPIRGASFCTLLQNLGPENAATLLLFALTENKILIHSLRPAVLTSAAEALNAMIFPFHWQCPYIPLCPL---SLADMLSAPCPFIVGVDSRYFDL---YEPPPDVVSVDLDTNTISQTEERKSL---------------NWRLLPK-------------------KPCKNLMNTLTNLHQQIVAMNHRSMEDPVLESGIFDLDFKRRARQQQLEMEIQEAFLRFM--ASILKGYRTYLRPITQAPSEKATDARSLFDLQGFLKSRDRAYNKFYSQMMRTQMFIRFIE 631          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Nematostella
Match: EDO49267 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH02])

HSP 1 Score: 412.149 bits (1058), Expect = 1.860e-119
Identity = 233/590 (39.49%), Postives = 349/590 (59.15%), Query Frame = 2
Query:  191 RLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERRSPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKRIRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIK-RRSNGKDSIWPTLLGSSVENTN-----SVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVR-KSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKP--SQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDF 1933
            RL+DY+ +VG+R P      ++  P LLRR+P  DH +  LP DVV+FCQP+ C +T + + + ++ N+F+FTLTDK+T +VRYG+C+N +RP  +                    +    + G+   SR SK  R    +LTS+ IVSHH  F  F+ CL +L+++I   D R+   R +  D+  P L   SV   +     S I   + EIETWI RLL +P PV G T++++ LL       + FA P+N RFPL+DFP+H   EL+GVE  LK+   +LLE KV++QS++YN L+ S++AL  LLYPLEYMFPI  LLPT M +AEQL  AP+P++ G+P+SF   + K F++P D+W FDLD  ++        +P +   ++L   P  +  +LI+ +K AL+      ++S   + V NL+    N     L P  +  + S    ++FGND DSVDV+ RVA+V FF S ++L    + TR L+L+PRP+VA Q   F+KSR   SDFV  L+++Q+V++F EW ++P NT + +I    Y+P +IGDK KW+ + LE + ++VY +     L    + D+
Sbjct:   12 RLVDYVILVGVRTPTLD---STETPRLLRRFPEKDHDDFPLPPDVVFFCQPEAC-NTVSKKFSLREANSFIFTLTDKDTNKVRYGVCMNIYRPCSQ---------------FHFRDDQDGIKDGKRKRSR-SKNKREICMTLTSICIVSHHSFFATFRECLFVLRKMI---DSRVGGERYDKNDNFIPGLHSWSVFTCSEDPKLSPIATQMDEIETWIQRLLLAPAPVIGRTKVDMELLCPESYPSLTFAYPENTRFPLIDFPVHIPMELLGVETCLKVLTCILLEQKVVIQSRDYNALTMSVIALTRLLYPLEYMFPIIPLLPTCMANAEQLLLAPTPYVIGVPASFFRYKSKDFQIPGDLWVFDLDSSKV-------KIPMSA--DELPDLPEEEGQVLIRRLKMALQ------ALSMPPQTVGNLE----NAAPDGLLPDNTSGDASIEEHMVFGNDVDSVDVAVRVAIVNFFMSPTVLCGLQEHTRTLRLFPRPVVALQKGPFLKSRPKPSDFVAALAESQSVEYFAEWLVSPTNTAYLKINKGIYNPCVIGDKPKWYSEHLETVLYQVYDKESKLCLEPWIDVDY 559          

HSP 2 Score: 136.732 bits (343), Expect = 4.373e-32
Identity = 92/327 (28.13%), Postives = 162/327 (49.54%), Query Frame = 2
Query: 3719 DSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELI----QAIQKHSEKFRSVIEPDT-----SQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            D     R YLF+ L    +RS +WDNM FWE++F DAV  ER+ +G+DQ   ++++RY  L   E++RLE  ED+LL   L N +AFM+    + V ++      +G+S      +  ++ ++  I + R  D   +T+      V    V C +     + V++   DC+LLR+ +G I  RWW E+ +  T   K  V+C+  R   + ++S+   KK K L     + +Q+ +E+ R    P+T      +F V D  +   G L++   G+          +D  ++R C T+K   F+++       + +Y++  +++ N I
Sbjct: 1100 DRSTTGRRYLFEGLV--RERSPLWDNMDFWENIFLDAVAAERDAVGMDQGPTEMLERYKSLQPVEQKRLEEEEDKLLATTLYNTVAFMLHWMCN-VIVRRTVFGNVGRSWKGYAVAPRMDAVLLFI-FFRALDDSDVTSARHAVVVGFGHVMCLMILFNRMTVSD---DCILLRTGAGVIIERWWYEKMVNITYCPKTKVLCLWYRRGEDTLLSKFCTKKCKALYFCIKECMQRAAERLRG---PNTGPQLGGEFLVEDISSGEEGKLQVTLEGIGLKFATRRVHIDLCHIRNCSTQK-GEFVLDEFGKCKTSFSYQLI-AFTCNQI 1414          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Nematostella
Match: EDO30806 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZQ4])

HSP 1 Score: 55.0694 bits (131), Expect = 1.527e-7
Identity = 39/156 (25.00%), Postives = 78/156 (50.00%), Query Frame = 2
Query:  812 IETWIFRLLSS-PVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDL 1276
            +E++I+ LL   P+P  G T +  + + E+    +    P  +  PL D+ L  +F  +GVE  ++LF  VLLE++++L S +++        +  L++P  +      +LP  +   +    AP PFI G+  +    + + ++PS+     +D+
Sbjct:  223 LESYIYNLLYEVPLPPPGRT-MRFNCVKES----ITCQRPGPNELPLFDYSLKEMFSWLGVENVVELFTCVLLEHQILLLSNSFHRFMLVAEGVVTLIFPFIWQHVYVPILPAAL---QHFLDAPVPFIMGLYCASEDFKANLQLPSEASLCLVDI 370          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Medaka
Match: madd (MAP kinase activating death domain [Source:NCBI gene;Acc:101163236])

HSP 1 Score: 497.664 bits (1280), Expect = 4.009e-148
Identity = 269/611 (44.03%), Postives = 384/611 (62.85%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIER-------------RSPIKVTRVXXXXXXXXXXXXXXXFQG--GRESM--SRQSKR------IRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSN-----GKDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D ++FVF LTDK++   RYGIC+NF+R  +R              +P K    + E  + +S A  ++       ES+  S Q +R       R R  +LTSL I+SH+P F  F+ CL+ILK +++   +R+ +R+       +D++W    G+ SVE   S +  D+ +IE+W++RLL SPVPVA   R+++ +LP  L + + FALPDN RF LVDFPLH   EL+GV+  L++   VLLE+KVILQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FK+P DVW  DLD        + K++  TN ++   LP P + + L KH+KQAL       S+S  ++P+ NL++     +L  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  +S F +KLS TQAV+F+ EW ++P N  F +I +  +DP LIGDK KW+   L+ + ++VY + GS
Sbjct:    1 MEKKKMCPRLLDYLVVVGARQP--SSDSVAQTPQLLRRYPLEDHPDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDSSFVFMLTDKDSSTTRYGICVNFYRSFQRGHHRPRGDKSGHTETPSKAAETTGEVSDGSSGASSTVLPAPTSTESVPPSPQHRRNAAKVAARNRNSTLTSLCILSHYPFFSTFRECLNILKRLVDCCSQRLTQRAGLPRATQRDTMWRVFTGALSVEEKGSQLLADLRDIESWVYRLLRSPVPVASQRRVDVEVLPHELKRALTFALPDNSRFTLVDFPLHLPLELLGVDACLQVLSCVLLEHKVILQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKIPDDVWLVDLD--------SSKVIAPTNAESLPPLPEP-EASELKKHLKQALA------SMSLNTQPILNLEKFQEGQDLSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRSPFAEKLSHTQAVEFYGEWALSPTNLAFQRIHNNVFDPSLIGDKPKWYAHQLQSVVYRVY-DGGS 593          

HSP 2 Score: 211.46 bits (537), Expect = 1.709e-54
Identity = 112/318 (35.22%), Postives = 182/318 (57.23%), Query Frame = 2
Query: 3737 RMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            R+YL + L GK +RS +WD M FWED + DAV  ERE +G+DQ  +++++RY  L  ++++RLE +EDRLL  +L+N IA+M+M++V+   I+ K R ++GKSHI L  S  IN+++D +  + G ++    + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E   P TK +    +K+  A+ I
Sbjct: 1183 RIYLCEGLLGK-ERSTLWDQMQFWEDAYLDAVMLEREGMGMDQGPQEMIERYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMLKVNKNDIRKKVRRLMGKSHIGLTYSQEINEILDKLANMNGRELSTRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELSHIKKCNTVK-GVFVLEEFVPETKEVVIHKYKTPMAHQI 1498          

HSP 3 Score: 63.5438 bits (153), Expect = 2.094e-9
Identity = 32/113 (28.32%), Postives = 61/113 (53.98%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV + S   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  856 ENQQFLKEVVQSVLDGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDARQEIIRDVEV-SRKVYKGMLDILKCTVSSLEQSYTNAGLGGMASVFSLLEIARTHYQTK 967          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Medaka
Match: madd (MAP kinase activating death domain [Source:NCBI gene;Acc:101163236])

HSP 1 Score: 494.582 bits (1272), Expect = 1.168e-146
Identity = 265/629 (42.13%), Postives = 375/629 (59.62%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR--------------------------------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKR---IRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSN-----GKDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D ++FVF LTDK++   RYGIC+NF+R  +R                                       +P     V        S         GR    R++      R R  +LTSL I+SH+P F  F+ CL+ILK +++   +R+ +R+       +D++W    G+ SVE   S +  D+ +IE+W++RLL SPVPVA   R+++ +LP  L + + FALPDN RF LVDFPLH   EL+GV+  L++   VLLE+KVILQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FK+P DVW  DLD        + K++  TN ++   LP P + + L KH+KQAL       S+S  ++P+ NL++     +L  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  +S F +KLS TQAV+F+ EW ++P N  F +I +  +DP LIGDK KW+   L+ + ++VY + GS
Sbjct:    1 MEKKKMCPRLLDYLVVVGARQP--SSDSVAQTPQLLRRYPLEDHPDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDSSFVFMLTDKDSSTTRYGICVNFYRSFQRGHHRPRGDKSGHTETPSKAAETTGEVSDGSSGASSTVLPAPTSTESVPPPASAEQSEQAGDELNSGRSPQHRRNAAKVAARNRNSTLTSLCILSHYPFFSTFRECLNILKRLVDCCSQRLTQRAGLPRATQRDTMWRVFTGALSVEEKGSQLLADLRDIESWVYRLLRSPVPVASQRRVDVEVLPHELKRALTFALPDNSRFTLVDFPLHLPLELLGVDACLQVLSCVLLEHKVILQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKIPDDVWLVDLD--------SSKVIAPTNAESLPPLPEP-EASELKKHLKQALA------SMSLNTQPILNLEKFQEGQDLSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRSPFAEKLSHTQAVEFYGEWALSPTNLAFQRIHNNVFDPSLIGDKPKWYAHQLQSVVYRVY-DGGS 611          

HSP 2 Score: 186.808 bits (473), Expect = 6.256e-47
Identity = 94/256 (36.72%), Postives = 148/256 (57.81%), Query Frame = 2
Query: 3773 DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTF 4516
            +RS +WD M FWED + DAV  ERE +G+DQ  +++++RY  L  ++++RLE +EDRLL  +L+N IA+M+M++V+   I+ K R ++GKSHI L  S  IN+++D +  + G ++    + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+  
Sbjct: 1298 ERSTLWDQMQFWEDAYLDAVMLEREGMGMDQGPQEMIERYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMLKVNKNDIRKKVRRLMGKSHIGLTYSQEINEILDKLANMNGRELSTRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINL 1553          

HSP 3 Score: 63.929 bits (154), Expect = 1.908e-9
Identity = 32/113 (28.32%), Postives = 61/113 (53.98%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV + S   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  939 ENQQFLKEVVQSVLDGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDARQEIIRDVEV-SRKVYKGMLDILKCTVSSLEQSYTNAGLGGMASVFSLLEIARTHYQTK 1050          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Medaka
Match: madd (MAP kinase activating death domain [Source:NCBI gene;Acc:101163236])

HSP 1 Score: 494.582 bits (1272), Expect = 1.399e-146
Identity = 265/629 (42.13%), Postives = 375/629 (59.62%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR--------------------------------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKR---IRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSN-----GKDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D ++FVF LTDK++   RYGIC+NF+R  +R                                       +P     V        S         GR    R++      R R  +LTSL I+SH+P F  F+ CL+ILK +++   +R+ +R+       +D++W    G+ SVE   S +  D+ +IE+W++RLL SPVPVA   R+++ +LP  L + + FALPDN RF LVDFPLH   EL+GV+  L++   VLLE+KVILQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FK+P DVW  DLD        + K++  TN ++   LP P + + L KH+KQAL       S+S  ++P+ NL++     +L  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  +S F +KLS TQAV+F+ EW ++P N  F +I +  +DP LIGDK KW+   L+ + ++VY + GS
Sbjct:    1 MEKKKMCPRLLDYLVVVGARQP--SSDSVAQTPQLLRRYPLEDHPDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDSSFVFMLTDKDSSTTRYGICVNFYRSFQRGHHRPRGDKSGHTETPSKAAETTGEVSDGSSGASSTVLPAPTSTESVPPPASAEQSEQAGDELNSGRSPQHRRNAAKVAARNRNSTLTSLCILSHYPFFSTFRECLNILKRLVDCCSQRLTQRAGLPRATQRDTMWRVFTGALSVEEKGSQLLADLRDIESWVYRLLRSPVPVASQRRVDVEVLPHELKRALTFALPDNSRFTLVDFPLHLPLELLGVDACLQVLSCVLLEHKVILQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKIPDDVWLVDLD--------SSKVIAPTNAESLPPLPEP-EASELKKHLKQALA------SMSLNTQPILNLEKFQEGQDLSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRSPFAEKLSHTQAVEFYGEWALSPTNLAFQRIHNNVFDPSLIGDKPKWYAHQLQSVVYRVY-DGGS 611          

HSP 2 Score: 196.438 bits (498), Expect = 7.517e-50
Identity = 103/295 (34.92%), Postives = 169/295 (57.29%), Query Frame = 2
Query: 3773 DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVE----ILDPNTK 4621
            +RS +WD M FWED + DAV  ERE +G+DQ  +++++RY  L  ++++RLE +EDRLL  +L+N IA+M+M++V+   I+ K R ++GKSHI L  S  IN+++D +  + G ++    + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E    ILD + +
Sbjct: 1298 ERSTLWDQMQFWEDAYLDAVMLEREGMGMDQGPQEMIERYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMLKVNKNDIRKKVRRLMGKSHIGLTYSQEINEILDKLANMNGRELSTRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELSHIKKCNTVK-GVFVLEEFAFILDADAE 1591          

HSP 3 Score: 63.5438 bits (153), Expect = 2.165e-9
Identity = 32/113 (28.32%), Postives = 61/113 (53.98%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV + S   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  939 ENQQFLKEVVQSVLDGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDARQEIIRDVEV-SRKVYKGMLDILKCTVSSLEQSYTNAGLGGMASVFSLLEIARTHYQTK 1050          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Medaka
Match: madd (MAP kinase activating death domain [Source:NCBI gene;Acc:101163236])

HSP 1 Score: 494.967 bits (1273), Expect = 1.820e-146
Identity = 265/629 (42.13%), Postives = 375/629 (59.62%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR--------------------------------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKR---IRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSN-----GKDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D ++FVF LTDK++   RYGIC+NF+R  +R                                       +P     V        S         GR    R++      R R  +LTSL I+SH+P F  F+ CL+ILK +++   +R+ +R+       +D++W    G+ SVE   S +  D+ +IE+W++RLL SPVPVA   R+++ +LP  L + + FALPDN RF LVDFPLH   EL+GV+  L++   VLLE+KVILQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FK+P DVW  DLD        + K++  TN ++   LP P + + L KH+KQAL       S+S  ++P+ NL++     +L  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  +S F +KLS TQAV+F+ EW ++P N  F +I +  +DP LIGDK KW+   L+ + ++VY + GS
Sbjct:    1 MEKKKMCPRLLDYLVVVGARQP--SSDSVAQTPQLLRRYPLEDHPDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDSSFVFMLTDKDSSTTRYGICVNFYRSFQRGHHRPRGDKSGHTETPSKAAETTGEVSDGSSGASSTVLPAPTSTESVPPPASAEQSEQAGDELNSGRSPQHRRNAAKVAARNRNSTLTSLCILSHYPFFSTFRECLNILKRLVDCCSQRLTQRAGLPRATQRDTMWRVFTGALSVEEKGSQLLADLRDIESWVYRLLRSPVPVASQRRVDVEVLPHELKRALTFALPDNSRFTLVDFPLHLPLELLGVDACLQVLSCVLLEHKVILQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKIPDDVWLVDLD--------SSKVIAPTNAESLPPLPEP-EASELKKHLKQALA------SMSLNTQPILNLEKFQEGQDLSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRSPFAEKLSHTQAVEFYGEWALSPTNLAFQRIHNNVFDPSLIGDKPKWYAHQLQSVVYRVY-DGGS 611          

HSP 2 Score: 204.142 bits (518), Expect = 3.185e-52
Identity = 106/306 (34.64%), Postives = 174/306 (56.86%), Query Frame = 2
Query: 3773 DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +RS +WD M FWED + DAV  ERE +G+DQ  +++++RY  L  ++++RLE +EDRLL  +L+N IA+M+M++V+   I+ K R ++GKSHI L  S  IN+++D +  + G ++    + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+     ++  F++  ++++C T K   F++E   P TK +    +K+  A+ I
Sbjct: 1298 ERSTLWDQMQFWEDAYLDAVMLEREGMGMDQGPQEMIERYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMLKVNKNDIRKKVRRLMGKSHIGLTYSQEINEILDKLANMNGRELSTRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQVFIELSHIKKCNTVK-GVFVLEEFVPETKEVVIHKYKTPMAHQI 1602          

HSP 3 Score: 63.929 bits (154), Expect = 1.983e-9
Identity = 32/113 (28.32%), Postives = 61/113 (53.98%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV + S   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  939 ENQQFLKEVVQSVLDGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDARQEIIRDVEV-SRKVYKGMLDILKCTVSSLEQSYTNAGLGGMASVFSLLEIARTHYQTK 1050          
BLAST of MAP kinase-activating death domain protein vs. Ensembl Medaka
Match: madd (MAP kinase activating death domain [Source:NCBI gene;Acc:101163236])

HSP 1 Score: 495.352 bits (1274), Expect = 1.968e-146
Identity = 265/629 (42.13%), Postives = 375/629 (59.62%), Query Frame = 2
Query:  167 MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALPQDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWRPIERR--------------------------------------SPIKVTRVXXXXXXXXXXXXXXXFQGGRESMSRQSKR---IRPRTHSLTSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSN-----GKDSIWPTLLGS-SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVVFALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNVLSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG----FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGS 1900
            M  + +CPRL+DYL +VG R P  S    +  P+LLRRYP  DH +  LP DVV+FCQP+GC+S    R + +D ++FVF LTDK++   RYGIC+NF+R  +R                                       +P     V        S         GR    R++      R R  +LTSL I+SH+P F  F+ CL+ILK +++   +R+ +R+       +D++W    G+ SVE   S +  D+ +IE+W++RLL SPVPVA   R+++ +LP  L + + FALPDN RF LVDFPLH   EL+GV+  L++   VLLE+KVILQS++YN LS S++A  A++YPLEYMFP+  LLPT M SAEQL  AP+P+I G+P+SF L +  FK+P DVW  DLD        + K++  TN ++   LP P + + L KH+KQAL       S+S  ++P+ NL++     +L  L P + + S     FNPLI+GND DSVDV+TRVAMVRFFNS ++L  +   TR L+L+PRP+VAFQ  +F+ SR  +S F +KLS TQAV+F+ EW ++P N  F +I +  +DP LIGDK KW+   L+ + ++VY + GS
Sbjct:    1 MEKKKMCPRLLDYLVVVGARQP--SSDSVAQTPQLLRRYPLEDHPDFPLPPDVVFFCQPEGCLSIRQRRVSLRDDSSFVFMLTDKDSSTTRYGICVNFYRSFQRGHHRPRGDKSGHTETPSKAAETTGEVSDGSSGASSTVLPAPTSTESVPPPASAEQSEQAGDELNSGRSPQHRRNAAKVAARNRNSTLTSLCILSHYPFFSTFRECLNILKRLVDCCSQRLTQRAGLPRATQRDTMWRVFTGALSVEEKGSQLLADLRDIESWVYRLLRSPVPVASQRRVDVEVLPHELKRALTFALPDNSRFTLVDFPLHLPLELLGVDACLQVLSCVLLEHKVILQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKIPDDVWLVDLD--------SSKVIAPTNAESLPPLPEP-EASELKKHLKQALA------SMSLNTQPILNLEKFQEGQDLSLLPPGRDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQSTSFLASRPRRSPFAEKLSHTQAVEFYGEWALSPTNLAFQRIHNNVFDPSLIGDKPKWYAHQLQSVVYRVY-DGGS 611          

HSP 2 Score: 200.675 bits (509), Expect = 3.579e-51
Identity = 107/309 (34.63%), Postives = 173/309 (55.99%), Query Frame = 2
Query: 3773 DRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCT--SKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKF---RSVIEPD---TSQFKVTDDVTKCSGSLKIDKNGLTFT---SDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            +RS +WD M FWED + DAV  ERE +G+DQ  +++++RY  L  ++++RLE +EDRLL  +L+N IA+M+M++V+   I+ K R ++GKSHI L  S  IN+++D +  + G ++    + SR    Q F V+ G D  GDI   E+  DC++LRS  G +  RWW E+ I  T   K  V+C+ RR   E  +++ Y KK +EL   ++   E+    +  I+P      +F V D  T   G L++   G+      S     F++  ++++C T K   F++E   P TK +    +K+  A+ I
Sbjct: 1315 ERSTLWDQMQFWEDAYLDAVMLEREGMGMDQGPQEMIERYLSLGEHDRKRLEDDEDRLLATLLHNMIAYMLMLKVNKNDIRKKVRRLMGKSHIGLTYSQEINEILDKLANMNGRELSTRPSGSRHIKKQTFVVHAGTDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKVFIELSHIKKCNTVK-GVFVLEEFVPETKEVVIHKYKTPMAHQI 1622          

HSP 3 Score: 63.929 bits (154), Expect = 1.890e-9
Identity = 32/113 (28.32%), Postives = 61/113 (53.98%), Query Frame = 2
Query: 2669 ENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEK 3007
            ENQ+ +++V   +L G     ++  +++ ++ENE  R  +LS++N+ ++   + ++  I DV + S   +  ++ I +  V+ +E SY      G  SVF  LE+  THY  K
Sbjct:  939 ENQQFLKEVVQSVLDGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDARQEIIRDVEV-SRKVYKGMLDILKCTVSSLEQSYTNAGLGGMASVFSLLEIARTHYQTK 1050          
BLAST of MAP kinase-activating death domain protein vs. Planmine SMEST
Match: SMESG000048179.1 (SMESG000048179.1)

HSP 1 Score: 2159.41 bits (5594), Expect = 0.000e+0
Identity = 1133/1139 (99.47%), Postives = 1133/1139 (99.47%), Query Frame = 2
Query: 1118 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKXXXXXXXXXXXXXXXXXQEILKSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKXXXXXXXXXXXXPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 4534
            MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL      NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD
Sbjct:    1 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL------NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 1133          
BLAST of MAP kinase-activating death domain protein vs. Planmine SMEST
Match: SMESG000048179.1 (SMESG000048179.1)

HSP 1 Score: 2158.26 bits (5591), Expect = 0.000e+0
Identity = 1142/1183 (96.53%), Postives = 1143/1183 (96.62%), Query Frame = 2
Query: 1118 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKXXXXXXXXXXXXXXXXXQEILKSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKXXXXXXXXXXXXPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI 4666
            MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL      NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASI                                GVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYS + I
Sbjct:    1 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL------NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASI--------------------------------GVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSYDQI 1145          
BLAST of MAP kinase-activating death domain protein vs. Planmine SMEST
Match: SMESG000048179.1 (SMESG000048179.1)

HSP 1 Score: 2075.44 bits (5376), Expect = 0.000e+0
Identity = 1101/1139 (96.66%), Postives = 1101/1139 (96.66%), Query Frame = 2
Query: 1118 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKXXXXXXXXXXXXXXXXXQEILKSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKXXXXXXXXXXXXPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 4534
            MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL      NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASI                                GVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD
Sbjct:    1 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL------NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASI--------------------------------GVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 1101          
BLAST of MAP kinase-activating death domain protein vs. Planmine SMEST
Match: SMESG000048179.1 (SMESG000048179.1)

HSP 1 Score: 2075.06 bits (5375), Expect = 0.000e+0
Identity = 1101/1139 (96.66%), Postives = 1101/1139 (96.66%), Query Frame = 2
Query: 1118 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKXXXXXXXXXXXXXXXXXQEILKSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKXXXXXXXXXXXXPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 4534
            MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL      NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASI                                GVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD
Sbjct:    1 MFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSFLLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL------NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASI--------------------------------GVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 1101          

HSP 2 Score: 90.1225 bits (222), Expect = 1.212e-17
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = 1
Query: 4534 LVCRFCQCQEMFHQKARPIYCRNLRPKHEESHLQNFQIVF 4653
            +VCRFCQCQEMFHQKARPIYCRNLRPKHEESHLQNFQIVF
Sbjct: 1110 VVCRFCQCQEMFHQKARPIYCRNLRPKHEESHLQNFQIVF 1149          

HSP 3 Score: 78.9518 bits (193), Expect = 3.292e-14
Identity = 38/38 (100.00%), Postives = 38/38 (100.00%), Query Frame = 2
Query: 4862 NCLLCSDKVLIAVDRSNKPFQQSFIATIKTSVLNKIRL 4975
            NCLLCSDKVLIAVDRSNKPFQQSFIATIKTSVLNKIRL
Sbjct: 1153 NCLLCSDKVLIAVDRSNKPFQQSFIATIKTSVLNKIRL 1190          
BLAST of MAP kinase-activating death domain protein vs. Planmine SMEST
Match: SMESG000048179.1 (SMESG000048179.1)

HSP 1 Score: 2074.67 bits (5374), Expect = 0.000e+0
Identity = 1092/1098 (99.45%), Postives = 1092/1098 (99.45%), Query Frame = 2
Query: 1241 MPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTXXXXXXXXXXXXXXXXNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKXXXXXXXXXXXXXXXXXQEILKSNPTYKPISERKPVIERSELINKLKEAHXXXXXXXXXXFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGXXXXXXXXXXXXXPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKXXXXXXXXXXXXPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 4534
            MPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL      NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD
Sbjct:    1 MPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFNILIKHIKQAL------NSISDKSEPVKNLDEVLNNVELQPLKPSQSEISGFNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVAFQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYDPRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDESGSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQIPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLSNKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSSNSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVIERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKGSTPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIESEDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGMNSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWDLIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNSDIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDISAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDGSFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTGKNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEKQRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDESANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEIHSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYCKKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTFTSDNTD 1092          

HSP 2 Score: 90.5077 bits (223), Expect = 1.064e-17
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = 1
Query: 4534 LVCRFCQCQEMFHQKARPIYCRNLRPKHEESHLQNFQIVF 4653
            +VCRFCQCQEMFHQKARPIYCRNLRPKHEESHLQNFQIVF
Sbjct: 1101 VVCRFCQCQEMFHQKARPIYCRNLRPKHEESHLQNFQIVF 1140          

HSP 3 Score: 78.9518 bits (193), Expect = 3.146e-14
Identity = 38/38 (100.00%), Postives = 38/38 (100.00%), Query Frame = 2
Query: 4862 NCLLCSDKVLIAVDRSNKPFQQSFIATIKTSVLNKIRL 4975
            NCLLCSDKVLIAVDRSNKPFQQSFIATIKTSVLNKIRL
Sbjct: 1144 NCLLCSDKVLIAVDRSNKPFQQSFIATIKTSVLNKIRL 1181          
The following BLAST results are available for this feature:
BLAST of MAP kinase-activating death domain protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MADD3.482e-14844.24MAP kinase activating death domain [Source:HGNC Sy... [more]
MADD4.527e-14844.24MAP kinase activating death domain [Source:HGNC Sy... [more]
MADD4.837e-14844.24MAP kinase activating death domain [Source:HGNC Sy... [more]
MADD6.264e-14844.24MAP kinase activating death domain [Source:HGNC Sy... [more]
MADD1.958e-14744.24MAP kinase activating death domain [Source:HGNC Sy... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
aex-34.398e-12840.98MAP kinase-activating death domain protein [Sourc... [more]
aex-34.598e-12840.98MAP kinase-activating death domain protein [Sourc... [more]
lst-61.342e-629.68Lateral Signaling Target [Source:UniProtKB/TrEMBL... [more]
mtm-57.535e-629.35Myotubularin-related protein 5 [Source:UniProtKB/... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rab3-GEF8.605e-11540.83gene:FBgn0030613 transcript:FBtr0330400[more]
Rab3-GEF8.915e-11540.83gene:FBgn0030613 transcript:FBtr0307210[more]
Rab3-GEF1.256e-11440.83gene:FBgn0030613 transcript:FBtr0307209[more]
Rab3-GEF1.547e-11440.83gene:FBgn0030613 transcript:FBtr0307208[more]
Rab3-GEF2.008e-11440.83gene:FBgn0030613 transcript:FBtr0307207[more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX119977.19.406e-15044.92MAP-kinase activating death domain [Source:NCBI ge... [more]
madd3.559e-14844.61MAP-kinase activating death domain [Source:ZFIN;Ac... [more]
BX119977.15.928e-14844.61MAP-kinase activating death domain [Source:NCBI ge... [more]
BX119977.12.564e-14743.59MAP-kinase activating death domain [Source:NCBI ge... [more]
BX119977.15.171e-14743.31MAP-kinase activating death domain [Source:NCBI ge... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
madd4.953e-15045.48MAP-kinase activating death domain [Source:NCBI ge... [more]
madd7.611e-15045.48MAP-kinase activating death domain [Source:NCBI ge... [more]
madd7.747e-15045.48MAP-kinase activating death domain [Source:NCBI ge... [more]
madd1.414e-14944.68MAP-kinase activating death domain [Source:NCBI ge... [more]
madd1.503e-14944.68MAP-kinase activating death domain [Source:NCBI ge... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Madd8.478e-15543.83MAP-kinase activating death domain [Source:MGI Sym... [more]
Madd8.891e-15343.83MAP-kinase activating death domain [Source:MGI Sym... [more]
Madd3.472e-15243.83MAP-kinase activating death domain [Source:MGI Sym... [more]
Madd3.783e-15243.83MAP-kinase activating death domain [Source:MGI Sym... [more]
Madd3.941e-15243.83MAP-kinase activating death domain [Source:MGI Sym... [more]
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BLAST of MAP kinase-activating death domain protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q80U28|MADD_MOUSE4.314e-15143.35MAP kinase-activating death domain protein OS=Mus ... [more]
sp|O08873|MADD_RAT2.406e-15042.18MAP kinase-activating death domain protein OS=Ratt... [more]
sp|Q8WXG6|MADD_HUMAN1.600e-14644.24MAP kinase-activating death domain protein OS=Homo... [more]
sp|O02626|MADD_CAEEL5.849e-12740.98MAP kinase-activating death domain protein OS=Caen... [more]
sp|Q9VXY2|MADD_DROME2.013e-11340.83MAP kinase-activating death domain protein OS=Dros... [more]
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BLAST of MAP kinase-activating death domain protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7VC382.451e-17645.80UDENN domain-containing protein (Fragment) OS=Capi... [more]
A0A2T7PJ461.272e-17143.04UDENN domain-containing protein OS=Pomacea canalic... [more]
A0A210Q2841.036e-17044.59MAP kinase-activating death domain protein OS=Mizu... [more]
V4ALC22.656e-16844.07UDENN domain-containing protein OS=Lottia gigantea... [more]
A0A433TIA09.072e-16845.34UDENN domain-containing protein (Fragment) OS=Elys... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
madd2.726e-13842.78MAP-kinase activating death domain [Source:ZFIN;Ac... [more]
sbf14.817e-1623.61SET binding factor 1 [Source:ZFIN;Acc:ZDB-GENE-040... [more]
dennd4b1.523e-1423.74DENN domain containing 4B [Source:NCBI gene;Acc:10... [more]
dennd4b1.863e-1423.74DENN domain containing 4B [Source:NCBI gene;Acc:10... [more]
sbf26.827e-1432.47SET binding factor 2 [Source:ZFIN;Acc:ZDB-GENE-030... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sbf14.734e-2023.29SET binding factor 1 [Source:ZFIN;Acc:ZDB-GENE-040... [more]
ENSPMAT00000010382.12.712e-1724.73pep scaffold:Pmarinus_7.0:GL482471:369374:413667:1... [more]
dennd4a1.209e-1223.50DENN/MADD domain containing 4A [Source:ZFIN;Acc:ZD... [more]
dennd5a1.912e-1224.22DENN/MADD domain containing 5A [Source:ZFIN;Acc:ZD... [more]
dennd4a1.043e-923.82DENN/MADD domain containing 4A [Source:ZFIN;Acc:ZD... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO492671.860e-11939.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO308061.527e-725.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of MAP kinase-activating death domain protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
madd4.009e-14844.03MAP kinase activating death domain [Source:NCBI ge... [more]
madd1.168e-14642.13MAP kinase activating death domain [Source:NCBI ge... [more]
madd1.399e-14642.13MAP kinase activating death domain [Source:NCBI ge... [more]
madd1.820e-14642.13MAP kinase activating death domain [Source:NCBI ge... [more]
madd1.968e-14642.13MAP kinase activating death domain [Source:NCBI ge... [more]
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BLAST of MAP kinase-activating death domain protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000048179.10.000e+099.47SMESG000048179.1[more]
SMESG000048179.10.000e+096.53SMESG000048179.1[more]
SMESG000048179.10.000e+096.66SMESG000048179.1[more]
SMESG000048179.11.212e-1796.66SMESG000048179.1[more]
SMESG000048179.11.064e-1799.45SMESG000048179.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020839 ID=SMED30020839|Name=MAP kinase-activating death domain protein|organism=Schmidtea mediterranea sexual|type=transcript|length=5320bp
AAACCCACATCTTTAAATATTTGAAATTACATCTGATGTAATATTTAATG
CTATTCCTTAGATATCCTTACTAGCATGGTTATACAAATCATTCAATATA
AATAATCATTTTGGATATGCTGAGACCTTCAAAATAAAACAATTTATTTG
GAGAGAGTTATAATAAATGGCCAATGAAAGCATTTGTCCAAGATTAATAG
ATTATTTGTCAATAGTTGGATTACGTAATCCTAACAGATCAGGACATAAA
AACAGCGTGGTACCTGAATTGCTGAGACGTTATCCTCACACTGATCACAA
AAATGGTGCACTACCTCAGGATGTAGTGTATTTCTGCCAACCTGACGGCT
GTATTAGCACATGTACCTCGCGAAATGCAGCAAAGGATTTTAATACTTTT
GTATTTACTCTTACCGATAAAGAAACTGGACGTGTTCGATATGGAATATG
TTTGAATTTCTGGAGACCAATTGAACGACGTAGTCCAATAAAGGTTACCC
GAGTATCATTGGAACGACTCGAGAGTGCTAGCAGTGCTGAAATGTCGATG
TTTCAGGGTGGCAGAGAATCGATGTCACGCCAGAGCAAACGCATCCGACC
AAGAACCCATTCATTAACCTCACTATTAATTGTCAGTCATCATCCCTGCT
TTAAGGGCTTTCAAACTTGCTTGTCGATCCTGAAAGAAATAATCGAGAAA
TTCGATGAAAGAATCAAACGAAGGAGCAATGGAAAGGACTCGATTTGGCC
GACTCTTTTGGGGTCATCTGTAGAAAATACAAACAGTGTCATTCACCATG
ATGTAATGGAAATTGAAACGTGGATTTTTCGATTGCTCAGTTCCCCAGTT
CCTGTGGCTGGAATCACAAGGATTGAACTGAGCCTTTTACCAGAAACCCT
CATGAAACCGGTAGTATTTGCCCTTCCCGATAATGATAGATTTCCCCTTG
TGGATTTCCCTTTGCATACGATATTCGAACTAATGGGAGTTGAATTCGCT
CTCAAACTGTTCATTGCAGTCCTCCTTGAAAACAAGGTAATACTACAATC
TAAGAATTATAATGTACTGTCCACGTCAATCCTTGCATTGAAGGCTCTTC
TTTACCCATTAGAGTACATGTTTCCCATCAGTACATTATTGCCAACTCAT
ATGCCAAGCGCCGAACAACTGTTCACAGCCCCTTCTCCCTTCATATTTGG
TATTCCATCAAGTTTCCTATTAGTCAGGAAATCATTTAAAATGCCATCTG
ATGTTTGGTTTTTCGATTTAGATTTACGACAATTATTGAAGCATAATAAT
CACAAAATGGTTCCACAGACCAATATCGACAATGATCTTAGTCTTCCACC
GCCAGCGGATTTTAATATTCTTATCAAGCACATCAAACAGGCGCTCAAGG
TCTGCCAGGCACTCAATAGTATCAGTGATAAAAGTGAGCCAGTAAAGAAT
CTGGACGAAGTCCTGAACAATGTTGAATTGCAACCTTTGAAACCGAGCCA
GTCCGAGATATCGGGTTTCAATCCACTGATATTCGGCAATGATTCTGATT
CTGTCGACGTATCAACAAGAGTAGCAATGGTGAGATTTTTCAATTCTCAG
AGCATTCTGGGAAACTATACGAAATTCACTCGAATTCTCAAATTGTATCC
ACGACCGATTGTTGCTTTTCAAGTGGACAATTTCATTAAATCTAGAAGAG
TGCAATCGGATTTCGTAAAAAAGCTTTCAAAAACTCAGGCAGTTCAGTTT
TTCTGTGAATGGAATATTGCTCCAAAAAATACGGTTTTCTTTCAAATTTT
ATCCGAAAAATACGATCCGAGATTGATCGGGGACAAATCCAAATGGTTTG
ATCAATCTCTAGAGCCAATCAATTTCAAAGTGTACAGAGAAAATGGATCA
TGCACATTGCACTTACTATTCAATCAGGATTTCAATACAGAATCAGACTG
CCCTACAGATGAGAGTGGCAGTGATAGTGATCATATCAGTGATTCCAGTT
CATCTAATAGAGAATTGACAGAAACCTGCAGTGATCTAGCAGACAGCGGC
ATTAGTGAATGCCAACGACTAACAGTCGACCCAACAAAAATCTATTATCC
GCCAACATATCTACAAATTCCTTCAATAAATATCATTGAAAGCGAAAAAG
ATTCTATTTTCAAAATCAAAATGGAGTCAAATACCCGTAAATTCGTTGCA
TCCGACACAGAAGAAATGAACATTAAGAAAAGAGAACGAGAAGTGAAAAG
ACAGCATAATCTATCAAACAAACGGAAGTCTGTGGATAAAAATCAAAGCA
AAGATCCTAAGACATTCACAACAGGCAATGCGTATGATACGGACAATGAA
ACAAATACGAGTTGCACATCGGAGCCTTTAAAACAGACCTCATTGAGTGG
ATCGGCAGTCTCTTCCAACTCTTATTCAGAATTTTGGAATGAGACCAGCA
AAAAATTGTCCGGGTTTTTCGGCAGTCCTCTATCTCCATCAAAACCAAGC
CTGAGCCAAGAGATATTGAAATCAAATCCGACATATAAACCTATCAGCGA
GAGGAAGCCCGTAATTGAAAGAAGTGAGTTGATAAACAAATTAAAGGAAG
CACACTCTGATAGCCGGCGTAGCTCGACCTCAAGTTTCTTGTTTAAAGAC
AAAAAGTCAATTGACTATGAGAATCAGGAAACGATTCAACAAGTCACGGA
CGAGATACTGAAAGGCAGCACTCCCAATATCATATCAAAATATAGAATTA
AAAATATTGTTGAAAATGAGGCTTATCGGAATTTAATTCTTTCTCGTATA
AACAAAAATCTTGAGAAAAATATCAATTGCAAAGAAACATATATAAATGA
TGTCATGATTGAGAGTGAAGACCATTTTAACGCATTGGTTTGGATAAGTA
GGATCCTAGTGAACGGGATTGAATGCAGCTACAATAAAGGCATATGTGAT
GGATTCGGAAGCGTATTCGTGTTTCTAGAAATGCTATTTACTCATTATGT
AGAAAAGAAAGGAATGAACTCTTCAATATCCGTTTCATCGATGAATTTTA
GCCCAGTGACAAATGATGAAAAACCGCCTTACTTCGATGGATTACCAAAC
GGCAACATGGTGGCATCTATTGCCGATTTATGGAAATACACAACTCAATA
CACCGCCAATTGGGATCTGATCAAGGTTTCGATCTCTGAATCCTGGAAGG
ATATCCAAGAATATGCAGGAGTGCCATTAAATATCAATGGTGAGCTGTAT
GAAACCATCGAGATATCGTCTAAAAACGATATTAATGCTACCCATGGTGT
TTCCATGAGCAATTCTGACATTGATGAAACTCATTCGTTATTCTCCCCAA
GAGCTCCTCCTTCTCCCGGCAGCAGTGTCTTCAAACGAGGTTCGGTGAAT
GTTCCTCGTACACTAAACATACGATCAGATACATCCAGTTTGGTGTCATT
TAAGGATGATGATATCAGTGCTATTATAGATTACAGCATTCCCTATAAAA
GTACTGTCACATTCGGCTACAGGTTCAAACTAAACCAGCTCTATTTCATG
AAAGAACCGAAATTGCCGACTGAAATCAGCCCTTCGAATCGTGAAAAATC
GAGTCCAAGAGACGGCAGCTTTTTCAGTCGGATAATTAACAGCCCACAGA
AGACCTTTCAATCCAATAGCGCCCCAGTATCAGGAAACAACATTGATAAT
AATGGAGTCGTGGAAGGAGATTCAATGGCCAACCAAAGGATGTATTTATT
TCAGAAATTAACTGGTAAAAATGATCGTTCGAAAATATGGGATAATATGG
CATTTTGGGAAGATGTTTTTTACGATGCAGTTTGTCAAGAGAGAGAAATT
ATCGGACTAGATCAGGATTCAAAAGACCTTATGGATAGATATAATGGTCT
GGATATGAATGAGAAGCAAAGGCTGGAAAGGAATGAGGACCGATTATTGG
GGATTGTCTTAAATAACTTTATTGCATTTATGATAATGATGCGTGTGGAT
CCGGTGAAAATTAAGTCGAAAGCAAGATGCATGATAGGAAAAAGTCACAT
TGCACTTGATGAAAGTGCAAATATCAACAAATTAATTGATTCCATCAAAT
ATCTGAGAGGAAACGATGTTGACCTCCTGACAAACATGAGCCGACAGCAG
GATGTGCAACTATTTCCTGTCTATTGTGGAGTTGACAAAAAAGGAGATAT
CTGTGTAGCTGAAATTCATTCCGATTGTATGCTGTTGAGATCAATTTCTG
GTGCAATTAACGCTCGTTGGTGGCTTGAACGGTTCATCAAGTGCACCAGC
AAGAAAAATGTGATATGTATAACAAGACGAGAAAATAATGAAGTTGTTAT
GAGCCAGTTGTATTGTAAAAAGAGCAAAGAATTGATTCAAGCCATTCAGA
AACATTCAGAGAAATTTAGAAGTGTTATAGAGCCTGATACGTCACAGTTC
AAGGTTACGGATGACGTCACAAAGTGCAGTGGGTCTCTGAAAATCGATAA
AAATGGTCTCACCTTCACCTCGGATAACACCGATTGGTTTGTCGATTTTG
TCAATGTCAGGAGATGTTTCACCAAAAAGCCAGACCGATTTATTGTAGAA
ATCTTAGACCCAAACACGAAGAATCTCACCTACAGAATTTTCAAATCGTA
TTCGGCCAATTTGATTTTTCGACTGTTCCAAAGCCAAGTGCAAAAATTTG
ATTTAAAAACTTTTAAAGAGAATTCAAATGGCATTCATACGGTGCCATTT
AACGCGAATTGGGACAGTGATGAATGGGATAATCTATCGTCGATTATGAA
TGGTTCTTTATCTAGACAATCGAGCACTTCTCTTTTTGACGACTCTTTTT
AGCTACGATCAAATTGCCTATTGTGTTCTGACAAAGTTCTCATTGCTGTC
GACAGATCGAACAAACCTTTCCAACAGTCTTTTATCGCCACTATCAAAAC
CTCCGTTTTAAATAAAATTCGGTTGTGATGTAATGTTTCAATTTTCACAT
CTATCGCATTATCCATTCAGTGATAGAAGTTGTGGTTTTTATTTTTTATT
AAATTTGTGCTTTCGTCTCGATGTTTATTGGCTGGTTATAATGTGGATAT
ATCTAGATTTATTATTTATCTATATATTTATTTATTTTTCAATTAATTAT
TGTGTTCCGTATTTCACACAACATTCCATGTCAAGCGCTATGTAAAGATT
ATTATCTAATTGTTTATTTCTCGTTAAATGATATTATTGAAGGATGTTTA
GCAGGTTTTGGACAAGGACGTACGTGTCGGTATTATGAATGTTTTATTAT
TATTTCTTTCATTATCTTCA
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protein sequence of SMED30020839-orf-1

>SMED30020839-orf-1 ID=SMED30020839-orf-1|Name=SMED30020839-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1562bp
MANESICPRLIDYLSIVGLRNPNRSGHKNSVVPELLRRYPHTDHKNGALP
QDVVYFCQPDGCISTCTSRNAAKDFNTFVFTLTDKETGRVRYGICLNFWR
PIERRSPIKVTRVSLERLESASSAEMSMFQGGRESMSRQSKRIRPRTHSL
TSLLIVSHHPCFKGFQTCLSILKEIIEKFDERIKRRSNGKDSIWPTLLGS
SVENTNSVIHHDVMEIETWIFRLLSSPVPVAGITRIELSLLPETLMKPVV
FALPDNDRFPLVDFPLHTIFELMGVEFALKLFIAVLLENKVILQSKNYNV
LSTSILALKALLYPLEYMFPISTLLPTHMPSAEQLFTAPSPFIFGIPSSF
LLVRKSFKMPSDVWFFDLDLRQLLKHNNHKMVPQTNIDNDLSLPPPADFN
ILIKHIKQALKVCQALNSISDKSEPVKNLDEVLNNVELQPLKPSQSEISG
FNPLIFGNDSDSVDVSTRVAMVRFFNSQSILGNYTKFTRILKLYPRPIVA
FQVDNFIKSRRVQSDFVKKLSKTQAVQFFCEWNIAPKNTVFFQILSEKYD
PRLIGDKSKWFDQSLEPINFKVYRENGSCTLHLLFNQDFNTESDCPTDES
GSDSDHISDSSSSNRELTETCSDLADSGISECQRLTVDPTKIYYPPTYLQ
IPSINIIESEKDSIFKIKMESNTRKFVASDTEEMNIKKREREVKRQHNLS
NKRKSVDKNQSKDPKTFTTGNAYDTDNETNTSCTSEPLKQTSLSGSAVSS
NSYSEFWNETSKKLSGFFGSPLSPSKPSLSQEILKSNPTYKPISERKPVI
ERSELINKLKEAHSDSRRSSTSSFLFKDKKSIDYENQETIQQVTDEILKG
STPNIISKYRIKNIVENEAYRNLILSRINKNLEKNINCKETYINDVMIES
EDHFNALVWISRILVNGIECSYNKGICDGFGSVFVFLEMLFTHYVEKKGM
NSSISVSSMNFSPVTNDEKPPYFDGLPNGNMVASIADLWKYTTQYTANWD
LIKVSISESWKDIQEYAGVPLNINGELYETIEISSKNDINATHGVSMSNS
DIDETHSLFSPRAPPSPGSSVFKRGSVNVPRTLNIRSDTSSLVSFKDDDI
SAIIDYSIPYKSTVTFGYRFKLNQLYFMKEPKLPTEISPSNREKSSPRDG
SFFSRIINSPQKTFQSNSAPVSGNNIDNNGVVEGDSMANQRMYLFQKLTG
KNDRSKIWDNMAFWEDVFYDAVCQEREIIGLDQDSKDLMDRYNGLDMNEK
QRLERNEDRLLGIVLNNFIAFMIMMRVDPVKIKSKARCMIGKSHIALDES
ANINKLIDSIKYLRGNDVDLLTNMSRQQDVQLFPVYCGVDKKGDICVAEI
HSDCMLLRSISGAINARWWLERFIKCTSKKNVICITRRENNEVVMSQLYC
KKSKELIQAIQKHSEKFRSVIEPDTSQFKVTDDVTKCSGSLKIDKNGLTF
TSDNTDWFVDFVNVRRCFTKKPDRFIVEILDPNTKNLTYRIFKSYSANLI
FRLFQSQVQKFDLKTFKENSNGIHTVPFNANWDSDEWDNLSSIMNGSLSR
QSSTSLFDDSF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR043153DENN_C
IPR039980MADD
IPR037516Tripartite_DENN
IPR005112dDENN_dom
IPR001194cDENN_dom
IPR005113uDENN_dom
Vocabulary: molecular function
TermDefinition
GO:0016301kinase activity
Vocabulary: biological process
TermDefinition
GO:0016310phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005112dDENN domainSMARTSM00801dDENN_clscoord: 463..533
e-value: 1.4E-16
score: 71.1
IPR001194cDENN domainSMARTSM00799DENN_clscoord: 148..371
e-value: 4.9E-45
score: 165.6
IPR001194cDENN domainPFAMPF02141DENNcoord: 151..369
e-value: 3.7E-28
score: 98.8
IPR005113uDENN domainSMARTSM00800uDENN_clscoord: 7..99
e-value: 1.9E-19
score: 80.6
IPR005113uDENN domainPFAMPF03456uDENNcoord: 33..98
e-value: 3.1E-18
score: 66.3
IPR043153DENN domain, C-terminal lobeGENE3DG3DSA:3.40.50.11500coord: 262..415
e-value: 1.6E-25
score: 91.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 687..711
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 687..738
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 594..616
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 712..738
IPR039980MAP kinase-activating death domain proteinPANTHERPTHR13008MAP-KINASE ACTIVATING DEATH DOMAIN PROTEIN MADD /DENN/AEX-3 C.ELEGANScoord: 680..1500
coord: 3..655
IPR037516Tripartite DENN domainPROSITEPS50211DENNcoord: 12..540
score: 55.148