SMED30020834

Overview


Name	SMED30020834
Smed ID	SMED30020834
Length (bp)	1687

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30020834 (SMED30020834) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020834 (SMED30020834) in cells (dots) of each of the 12 neoblast clusters.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30020834

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30020834	SMESG000060160.1	dd_Smed_v4_10167_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30020834	SMESG000060160.1	dd_Smed_v4_10167_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30020834	SMESG000060160.1	dd_Smed_v4_10167_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30020834	SMESG000060160.1	dd_Smed_v4_10167_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30020834	SMESG000060160.1	dd_Smed_v4_10167_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30020834	SMESG000060160.1	dd_Smed_v4_10167_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30020834 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.001900:2490..11414 -	8357

Homology

[Close]

BLAST of SMED30020834 vs. Ensembl Human
Match: STXBP2 (syntaxin binding protein 2 [Source:HGNC Symbol;Acc:HGNC:11445])

HSP 1 Score: 88.5817 bits (218), Expect = 6.548e-20
Identity = 37/81 (45.68%), Postives = 60/81 (74.07%), Query Frame = -3

Query: 1317 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1550
            W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ +++DF+ TP   Y  AH+FF D
Sbjct:   12 WKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIKDFQGTPTFTYKAAHIFFTD 92

[Close]

BLAST of SMED30020834 vs. Ensembl Human
Match: STXBP2 (syntaxin binding protein 2 [Source:HGNC Symbol;Acc:HGNC:11445])

HSP 1 Score: 90.8929 bits (224), Expect = 6.010e-19
Identity = 40/99 (40.40%), Postives = 68/99 (68.69%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1604
            +  +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ +++DF+ TP   Y  AH+FF D
Sbjct:   10 VGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIKDFQGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Human
Match: STXBP2 (syntaxin binding protein 2 [Source:HGNC Symbol;Acc:HGNC:11445])

HSP 1 Score: 91.2781 bits (225), Expect = 1.135e-18
Identity = 40/99 (40.40%), Postives = 68/99 (68.69%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1604
            +  +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ +++DF+ TP   Y  AH+FF D
Sbjct:   10 VGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIKDFQGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Human
Match: STXBP1 (syntaxin binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11444])

HSP 1 Score: 81.6481 bits (200), Expect = 2.898e-18
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1550
            W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   14 WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDS 95

[Close]

BLAST of SMED30020834 vs. Ensembl Human
Match: STXBP1 (syntaxin binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11444])

HSP 1 Score: 82.0333 bits (201), Expect = 7.830e-18
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1550
            W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   14 WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDS 95

[Close]

BLAST of SMED30020834 vs. Ensembl Celegans
Match: unc-18 (Putative acetylcholine regulator unc-18 [Source:UniProtKB/Swiss-Prot;Acc:P34815])

HSP 1 Score: 79.337 bits (194), Expect = 2.677e-15
Identity = 33/82 (40.24%), Postives = 60/82 (73.17%), Query Frame = -3

Query: 1314 NIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVDG 1556
            + W +L++D  + ++++   KMH++ ++G+T+V+DL KRREP+P LEAIYLI+P  + I+ +++D+ +  +Y  AHVFF + 
Sbjct:   26 SAWNVLIVDTLAMRMLSSCCKMHNIMEEGITIVEDLNKRREPLPTLEAIYLIAPTAESIDKLIQDYCARNLYKCAHVFFTEA 107

[Close]

BLAST of SMED30020834 vs. Ensembl Celegans
Match: T07A9.10 (pep chromosome:WBcel235:IV:390512:393158:1 gene:WBGene00020298.1 transcript:T07A9.10.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T07A9.10)

HSP 1 Score: 68.9366 bits (167), Expect = 4.758e-12
Identity = 33/85 (38.82%), Postives = 58/85 (68.24%), Query Frame = -3

Query: 1317 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP------VYSKAHVFFVD 1550
            + +LVLD+ +  ++   L ++++F++GVTLV+DLT+ REP+P ++AIY+ISP  + I++++ DF           Y  AH+FF+D
Sbjct:   29 FSVLVLDKSAMVVVNSCLSLNEVFEEGVTLVEDLTRNREPMPSMDAIYIISPVAESIDILINDFSRKTKFNPGNSYRSAHIFFLD 113

[Close]

BLAST of SMED30020834 vs. Ensembl Celegans
Match: sly-1 (SLY1 homolog [Source:UniProtKB/TrEMBL;Acc:Q20364])

HSP 1 Score: 48.9062 bits (115), Expect = 8.095e-6
Identity = 27/75 (36.00%), Postives = 43/75 (57.33%), Query Frame = -3

Query: 1359 VFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE 1580
            V +    E +W++LVLD     II+ +L +  L + GVTL   +  RREP+  + A+Y +SP  + I+LM  D +
Sbjct:   26 VVNSGVREPVWKVLVLDRTGQDIISPLLPVKQLRELGVTLHLLIGARREPLTDVPAVYFVSPNDENIDLMSEDLK 100

[Close]

BLAST of SMED30020834 vs. Ensembl Celegans
Match: sly-1 (SLY1 homolog [Source:UniProtKB/TrEMBL;Acc:Q20364])

HSP 1 Score: 48.9062 bits (115), Expect = 9.358e-6
Identity = 27/75 (36.00%), Postives = 43/75 (57.33%), Query Frame = -3

Query: 1359 VFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE 1580
            V +    E +W++LVLD     II+ +L +  L + GVTL   +  RREP+  + A+Y +SP  + I+LM  D +
Sbjct:   26 VVNSGVREPVWKVLVLDRTGQDIISPLLPVKQLRELGVTLHLLIGARREPLTDVPAVYFVSPNDENIDLMSEDLK 100

[Close]

BLAST of SMED30020834 vs. Ensembl Fly
Match: Rop (gene:FBgn0004574 transcript:FBtr0310632)

HSP 1 Score: 81.2629 bits (199), Expect = 7.530e-16
Identity = 35/81 (43.21%), Postives = 60/81 (74.07%), Query Frame = -3

Query: 1317 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1550
            WR+LV+D+   ++++   KMH++  +G+TLV+D+ K+REP+P ++AIYLI+P  + +  ++RDFE  + P+Y  AHVFF +
Sbjct:   39 WRVLVVDKLGMRMVSACTKMHEISAEGITLVEDINKKREPLPTMDAIYLITPSDESVRGLIRDFENPARPMYRYAHVFFTE 119

[Close]

BLAST of SMED30020834 vs. Ensembl Fly
Match: Rop (gene:FBgn0004574 transcript:FBtr0073263)

HSP 1 Score: 81.2629 bits (199), Expect = 7.530e-16
Identity = 35/81 (43.21%), Postives = 60/81 (74.07%), Query Frame = -3

Query: 1317 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1550
            WR+LV+D+   ++++   KMH++  +G+TLV+D+ K+REP+P ++AIYLI+P  + +  ++RDFE  + P+Y  AHVFF +
Sbjct:   39 WRVLVVDKLGMRMVSACTKMHEISAEGITLVEDINKKREPLPTMDAIYLITPSDESVRGLIRDFENPARPMYRYAHVFFTE 119

[Close]

BLAST of SMED30020834 vs. Ensembl Zebrafish
Match: stxbp2 (syntaxin binding protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2482])

HSP 1 Score: 85.5001 bits (210), Expect = 4.094e-17
Identity = 42/97 (43.30%), Postives = 64/97 (65.98%), Query Frame = -3

Query: 1317 HREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP-VYSKAHVFFVD 1601
            + +I S V   + ++  W++L++D  S +I++   KM D+  +GVT+V+D++KRREP+P LEAIYLISP  K +  ++ DF  T   Y  AHVFF D
Sbjct:   11 NEKILSGVIKSVKKDGDWKVLIVDHISMRILSSCCKMSDIMAEGVTIVEDISKRREPIPSLEAIYLISPVPKSVHALIADFRETAFTYKAAHVFFTD 107

[Close]

BLAST of SMED30020834 vs. Ensembl Zebrafish
Match: stxbp1b (syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-060531-166])

HSP 1 Score: 82.8037 bits (203), Expect = 3.417e-16
Identity = 37/100 (37.00%), Postives = 67/100 (67.00%), Query Frame = -3

Query: 1314 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFES--TPVYSKAHVFFVDG 1604
            +  +I + V   + ++  W++LV+D+ S ++++   KM D+  +G+T+V+D+TK+REP+P +EAI+LI+P  + +E ++ DF     P+Y  AHVFF D 
Sbjct:   10 VGEKIMNDVIRKVKKKGEWKVLVVDKLSMRMVSSCCKMTDIMSEGITIVEDITKKREPLPTMEAIFLITPSDESVEGLINDFRDPRNPIYRGAHVFFTDA 109

[Close]

Query: 1314 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFES--TPVYSKAHVFFVDG 1604
            +  +I + V   + ++  W++LV+D+ S ++++   KM D+  +G+T+V+D+TK+REP+P +EAI+LI+P  + +E ++ DF     P+Y  AHVFF D 
Sbjct:   10 VGEKIMNDVIRKVKKKGEWKVLVVDKLSMRMVSSCCKMTDIMSEGITIVEDITKKREPLPTMEAIFLITPSDESVEGLINDFRDPRNPIYRGAHVFFTDA 109

[Close]

BLAST of SMED30020834 vs. Ensembl Zebrafish
Match: stxbp1a (syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-GENE-050626-106])

HSP 1 Score: 77.411 bits (189), Expect = 1.850e-14
Identity = 35/81 (43.21%), Postives = 57/81 (70.37%), Query Frame = -3

Query: 1317 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFES--TPVYSKAHVFFVD 1550
            W+ L++D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEAIYLI+P  K ++ ++ DF+   +  Y  AHVFF D
Sbjct:   28 WKALIVDQLSMRMLSSCCKMTDIMTEGITIVEDIMKRREPLPSLEAIYLITPTDKSVQTLINDFKDPHSAKYKGAHVFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Zebrafish
Match: stxbp1a (syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-GENE-050626-106])

HSP 1 Score: 77.0258 bits (188), Expect = 2.190e-14
Identity = 35/82 (42.68%), Postives = 57/82 (69.51%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFES--TPVYSKAHVFFVDG 1550
            W+ L++D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEAIYLI+P  K ++ ++ DF+   +  Y  AHVFF D 
Sbjct:   28 WKALIVDQLSMRMLSSCCKMTDIMTEGITIVEDIMKRREPLPSLEAIYLITPTDKSVQTLINDFKDPHSAKYKGAHVFFTDS 109

[Close]

BLAST of SMED30020834 vs. Ensembl Xenopus
Match: krt8.1 (keratin 8, gene 1 [Source:Xenbase;Acc:XB-GENE-876929])

HSP 1 Score: 84.7297 bits (208), Expect = 1.064e-16
Identity = 38/99 (38.38%), Postives = 66/99 (66.67%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1604
            +  +I + V   + ++  W++L++D  S +I++   KM D+ D+G+T+V+D+ KRREP+P LEAIY I+P +K +  ++ DF+    P+Y  AH+FF D
Sbjct:   10 VGDKIVAGVITSVKKDGEWKVLLVDHTSMRILSSCCKMSDMLDEGITIVEDVNKRREPIPSLEAIYFITPCEKSVRAIISDFKDMGAPMYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Xenopus
Match: stxbp1 (syntaxin binding protein 1 [Source:NCBI gene;Acc:779976])

HSP 1 Score: 81.2629 bits (199), Expect = 1.212e-15
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1550
            W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   14 WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPIPSLEAVYLITPSEKSVHSLISDFKDPPSSKYRAAHVFFTDS 95

[Close]

BLAST of SMED30020834 vs. Ensembl Xenopus
Match: stxbp1 (syntaxin binding protein 1 [Source:NCBI gene;Acc:779976])

HSP 1 Score: 81.2629 bits (199), Expect = 1.266e-15
Identity = 38/100 (38.00%), Postives = 65/100 (65.00%), Query Frame = -3

Query: 1314 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1604
            +  +I   V   + ++  W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   10 VGEKIMHDVIRKVKKKGEWKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPIPSLEAVYLITPSEKSVHSLISDFKDPPSSKYRAAHVFFTDS 109

[Close]

BLAST of SMED30020834 vs. Ensembl Xenopus
Match: stxbp1 (syntaxin binding protein 1 [Source:NCBI gene;Acc:779976])

HSP 1 Score: 81.2629 bits (199), Expect = 1.338e-15
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1550
            W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   14 WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPIPSLEAVYLITPSEKSVHSLISDFKDPPSSKYRAAHVFFTDS 95

[Close]

BLAST of SMED30020834 vs. Ensembl Xenopus
Match: stxbp1 (syntaxin binding protein 1 [Source:NCBI gene;Acc:779976])

HSP 1 Score: 81.2629 bits (199), Expect = 1.379e-15
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1550
            W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   14 WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPIPSLEAVYLITPSEKSVHSLISDFKDPPSSKYRAAHVFFTDS 95

[Close]

BLAST of SMED30020834 vs. Ensembl Mouse
Match: Stxbp2 (syntaxin binding protein 2 [Source:MGI Symbol;Acc:MGI:107370])

HSP 1 Score: 90.1225 bits (222), Expect = 1.731e-18
Identity = 40/99 (40.40%), Postives = 67/99 (67.68%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1604
            +  +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ ++ DF+ TP   Y  AH+FF D
Sbjct:   10 VGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIADFQGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Mouse
Match: Stxbp2 (syntaxin binding protein 2 [Source:MGI Symbol;Acc:MGI:107370])

HSP 1 Score: 90.1225 bits (222), Expect = 1.774e-18
Identity = 40/99 (40.40%), Postives = 67/99 (67.68%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1604
            +  +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ ++ DF+ TP   Y  AH+FF D
Sbjct:   10 VGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIADFQGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Mouse
Match: Stxbp1 (syntaxin binding protein 1 [Source:MGI Symbol;Acc:MGI:107363])

HSP 1 Score: 81.6481 bits (200), Expect = 9.406e-16
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1550
            W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   28 WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDS 109

[Close]

BLAST of SMED30020834 vs. Ensembl Mouse
Match: Stxbp3 (syntaxin binding protein 3 [Source:MGI Symbol;Acc:MGI:107362])

HSP 1 Score: 81.2629 bits (199), Expect = 9.876e-16
Identity = 38/99 (38.38%), Postives = 68/99 (68.69%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPV-PLEAIYLISPEKKKIELMLRDF--ESTPVYSKAHVFFVD 1604
            + R+I + VFD   +E  W+I++LDE++T++++   KM DL ++G+T+++++ K REPV  ++A+Y ISP  K ++  LRDF  +S   Y  A+++F D
Sbjct:   14 VWRKIKTAVFDDCRKEGEWKIMLLDEFTTKLLSSCCKMTDLLEEGITVIENIYKNREPVRQMKALYFISPTPKSVDCFLRDFGSKSEKKYKAAYIYFTD 112

[Close]

BLAST of SMED30020834 vs. Ensembl Mouse
Match: Stxbp1 (syntaxin binding protein 1 [Source:MGI Symbol;Acc:MGI:107363])

HSP 1 Score: 81.2629 bits (199), Expect = 1.091e-15
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVDG 1550
            W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+  P   Y  AHVFF D 
Sbjct:   28 WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDS 109

[Close]

BLAST of SMED30020834 vs. UniProt/SwissProt
Match: sp|Q29268|STXB2_PIG (Syntaxin-binding protein 2 (Fragment) OS=Sus scrofa OX=9823 GN=STXBP2 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.060e-18
Identity = 39/98 (39.80%), Postives = 64/98 (65.31%), Query Frame = -3

Query: 1320 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFV 1604
            +  +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ ++ DF   P   Y  AHVFF 
Sbjct:   10 VGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIXDFRGXPTXTYKAAHVFFT 107

[Close]

BLAST of SMED30020834 vs. UniProt/SwissProt
Match: sp|Q15833|STXB2_HUMAN (Syntaxin-binding protein 2 OS=Homo sapiens OX=9606 GN=STXBP2 PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 5.452e-18
Identity = 40/99 (40.40%), Postives = 68/99 (68.69%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1604
            +  +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ +++DF+ TP   Y  AH+FF D
Sbjct:   10 VGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIKDFQGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. UniProt/SwissProt
Match: sp|Q64324|STXB2_MOUSE (Syntaxin-binding protein 2 OS=Mus musculus OX=10090 GN=Stxbp2 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.241e-17
Identity = 40/99 (40.40%), Postives = 67/99 (67.68%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1604
            +  +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ ++ DF+ TP   Y  AH+FF D
Sbjct:   10 VGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIADFQGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. UniProt/SwissProt
Match: sp|Q62753|STXB2_RAT (Syntaxin-binding protein 2 OS=Rattus norvegicus OX=10116 GN=Stxbp2 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.328e-17
Identity = 40/96 (41.67%), Postives = 66/96 (68.75%), Query Frame = -3

Query: 1317 EICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1595
            +I S V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ ++ DF+ TP   Y  AH+FF D
Sbjct:   13 KILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIADFQGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. UniProt/SwissProt
Match: sp|Q28288|STXB2_CANLF (Syntaxin-binding protein 2 OS=Canis lupus familiaris OX=9615 GN=STXBP2 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 6.243e-17
Identity = 39/99 (39.39%), Postives = 66/99 (66.67%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVD 1604
            +  +I + V   + ++  W++L++D  S +I++   KM D+  +G+T+V+D+ KRREP+P LEAIYL+SP +K ++ ++ DF  TP   Y  AH+FF D
Sbjct:   10 VGEKILNGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIADFRGTPTFTYKAAHIFFTD 108

[Close]

BLAST of SMED30020834 vs. TrEMBL
Match: A0A2D4FWY2 (Uncharacterized protein (Fragment) OS=Micrurus corallinus OX=54390 PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 8.224e-19
Identity = 46/106 (43.40%), Postives = 69/106 (65.09%), Query Frame = -3

Query: 1296 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVDGEAGDLL 1604
            +  +I S V   + +E  W++L++D  ST+I++   KM DL  +G+T+++D+ KRREP+P LEAIYLISP +  +  ++ DF+STP   Y  AHVFF D    DL 
Sbjct:   10 VGEKILSDVIHSMKKEGEWKVLIMDHSSTRILSSCFKMSDLLAEGITIIEDINKRREPLPSLEAIYLISPIETSVRALINDFKSTPTFTYKAAHVFFTDTCPDDLF 115

[Close]

BLAST of SMED30020834 vs. TrEMBL
Match: A0A2D4FWZ1 (Uncharacterized protein (Fragment) OS=Micrurus corallinus OX=54390 PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.977e-18
Identity = 46/106 (43.40%), Postives = 69/106 (65.09%), Query Frame = -3

Query: 1296 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVDGEAGDLL 1604
            +  +I S V   + +E  W++L++D  ST+I++   KM DL  +G+T+++D+ KRREP+P LEAIYLISP +  +  ++ DF+STP   Y  AHVFF D    DL 
Sbjct:   38 VGEKILSDVIHSMKKEGEWKVLIMDHSSTRILSSCFKMSDLLAEGITIIEDINKRREPLPSLEAIYLISPIETSVRALINDFKSTPTFTYKAAHVFFTDTCPDDLF 143

[Close]

BLAST of SMED30020834 vs. TrEMBL
Match: A0A5J4NQ93 (Syntaxin-binding protein 1 OS=Paragonimus westermani OX=34504 GN=DEA37_0000528 PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.143e-18
Identity = 43/95 (45.26%), Postives = 68/95 (71.58%), Query Frame = -3

Query: 1314 EICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVPLEAIYLISPEKKKIELMLRDFESTP-VYSKAHVFFVDG 1595
            E+  ++  PL     W++LVLD  ST+I++   KMH++ + G+TLV+D+TKRRE +P+EAIYLI+P ++ I+L++ DF+S+   Y  AHVFF + 
Sbjct:   23 ELMEEIILPLKNSKDWKVLVLDRLSTRIVSSCCKMHEIMNNGITLVEDITKRREVLPIEAIYLITPTEQSIQLLMDDFQSSSNQYRFAHVFFTEA 117

[Close]

BLAST of SMED30020834 vs. TrEMBL
Match: A0A1S8X7V6 (Sec1 family protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_01303 PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 3.549e-17
Identity = 41/88 (46.59%), Postives = 64/88 (72.73%), Query Frame = -3

Query: 1320 VFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVPLEAIYLISPEKKKIELMLRDFES-TPVYSKAHVFFV 1580
            VF PLY+   W+IL++DE +T++++ + KMHD+ + G+TLV+ L K R P  +EAIY++ P +++++L LRDF S  P Y  AHVFF+
Sbjct:   15 VFQPLYQPGSWKILIMDERATRVVSSVYKMHDITEFGITLVESLYKVRRPFNMEAIYIMYPHRREVDLFLRDFSSEMPTYPAAHVFFL 102

[Close]

BLAST of SMED30020834 vs. TrEMBL
Match: A0A2D4JZP0 (Uncharacterized protein (Fragment) OS=Micrurus paraensis OX=1970185 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.594e-17
Identity = 44/98 (44.90%), Postives = 65/98 (66.33%), Query Frame = -3

Query: 1296 VFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTP--VYSKAHVFFVDGEAGDLL 1580
            V   + +E  W++L++D  ST+I++   KM DL  +G+T+++D+ KRREP+P LEAIYLISP +  +  ++ DF+STP   Y  AHVFF D    DL 
Sbjct:    1 VIHSMKKEGEWKVLIMDYSSTRILSSCFKMSDLLAEGITIIEDINKRREPLPSLEAIYLISPIETSVRALINDFKSTPTFTYKAAHVFFTDTCPDDLF 98

[Close]

BLAST of SMED30020834 vs. Ensembl Cavefish
Match: stxbp2 (syntaxin binding protein 2 [Source:NCBI gene;Acc:103039197])

HSP 1 Score: 77.7962 bits (190), Expect = 1.091e-14
Identity = 37/98 (37.76%), Postives = 63/98 (64.29%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPV-PLEAIYLISPEKKKIELMLRDF-ESTPVYSKAHVFFVD 1604
            +  +I + V   + ++  W++L++D  S +I++   KM D+  +GVT+V+D+ KRREP+  LEAIY ISP  K ++ ++ DF +S   Y  AH+FF +
Sbjct:   10 VGEKIINGVLKSIKKDGEWKVLIVDHISMRILSSCCKMSDIMAEGVTIVEDINKRREPISSLEAIYFISPVSKSVDGLISDFKDSAFTYKAAHIFFTE 107

[Close]

BLAST of SMED30020834 vs. Ensembl Cavefish
Match: stxbp1a (syntaxin binding protein 1 [Source:NCBI gene;Acc:103024456])

HSP 1 Score: 77.411 bits (189), Expect = 1.266e-14
Identity = 35/81 (43.21%), Postives = 58/81 (71.60%), Query Frame = -3

Query: 1317 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFES--TPVYSKAHVFFVD 1550
            W+ L++D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEAIYLI+P +K ++ ++ DF+   +  Y  AHVFF D
Sbjct:   28 WKALIVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPTLEAIYLITPTEKSVQTLVGDFKDPHSSKYRAAHVFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Cavefish
Match: stxbp1a (syntaxin binding protein 1 [Source:NCBI gene;Acc:103024456])

HSP 1 Score: 77.411 bits (189), Expect = 1.453e-14
Identity = 35/82 (42.68%), Postives = 58/82 (70.73%), Query Frame = -3

Query: 1314 WRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFES--TPVYSKAHVFFVDG 1550
            W+ L++D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEAIYLI+P +K ++ ++ DF+   +  Y  AHVFF D 
Sbjct:   28 WKALIVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPTLEAIYLITPTEKSVQTLVGDFKDPHSSKYRAAHVFFTDS 109

[Close]

BLAST of SMED30020834 vs. Ensembl Cavefish
Match: stxbp3 (syntaxin binding protein 3 [Source:NCBI gene;Acc:103044348])

HSP 1 Score: 76.6406 bits (187), Expect = 2.407e-14
Identity = 34/87 (39.08%), Postives = 58/87 (66.67%), Query Frame = -3

Query: 1296 IWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPV-PLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVDGEAGDLL 1553
            +W+I++LD ++T++++   KM DL  +G+T+V+DL K REPV  ++A+Y +SP  K ++  + DF+  P Y  A+V+F D    DL 
Sbjct:   30 VWKIMILDSFTTRLLSSCCKMSDLMAEGITIVEDLFKSREPVLEMKAVYFMSPTTKCVDAFINDFKLKPKYKAAYVYFTDYCPDDLF 116

[Close]

BLAST of SMED30020834 vs. Ensembl Sea Lamprey
Match: stxbp1a (syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-GENE-050626-106])

HSP 1 Score: 82.8037 bits (203), Expect = 5.314e-17
Identity = 37/99 (37.37%), Postives = 66/99 (66.67%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV--YSKAHVFFVD 1604
            +  +I  +V   + ++  W++LV+D+ S ++++   KM D+  +G+T+V+D+ KRREP+P LEA+YLI+P +K +  ++ DF+   +  Y  AHVFF D
Sbjct:   11 VGEKIMKEVIQKVKKKGEWKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPIPSLEALYLITPSEKSVRALINDFKDASIFKYKGAHVFFTD 109

[Close]

BLAST of SMED30020834 vs. Ensembl Yeast
Match: SLY1 (Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex [Source:SGD;Acc:S000002597])

HSP 1 Score: 59.3066 bits (142), Expect = 1.003e-9
Identity = 31/90 (34.44%), Postives = 57/90 (63.33%), Query Frame = -3

Query: 1296 EENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVDGEAGDLL 1562
            +E IW++L+LD  ST  I+ +L+++DL   G+T+   + + R P+P + AIY +SP K+ I++++ D +S   YS+ ++ F      +LL
Sbjct:   49 QEIIWKVLILDIKSTATISSVLRVNDLLKAGITVHSLIKQDRSPLPDVPAIYFVSPTKENIDIIVNDLKSDK-YSEFYINFTSSLPRNLL 137

[Close]

BLAST of SMED30020834 vs. Ensembl Nematostella
Match: EDO43368 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYS1])

HSP 1 Score: 83.9593 bits (206), Expect = 3.933e-17
Identity = 43/85 (50.59%), Postives = 62/85 (72.94%), Query Frame = -3

Query: 1293 LVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFESTPV-YSKAHVFFVDGEAGDLLI 1541
            LVLD  ST++I+  +KMHDL D GVT+V++L KRREP+P LEAIY+++PE+  I L++ DF+  P+ Y  AH+FF +     LL+
Sbjct:    1 LVLDHLSTRMISSCVKMHDLIDNGVTVVENLEKRREPLPQLEAIYILTPEEDSISLLVADFKEYPLKYKGAHLFFTEVCPDSLLV 85

[Close]

BLAST of SMED30020834 vs. Ensembl Nematostella
Match: EDO43369 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYS2])

HSP 1 Score: 79.337 bits (194), Expect = 1.101e-15
Identity = 40/94 (42.55%), Postives = 66/94 (70.21%), Query Frame = -3

Query: 1317 ICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFES-TPVYSKAHVFFVD 1592
            I   VF PL++   W++LV+D  S ++++  LKMHDL + G+T+V+++  +REP+  LEAIYLI+P++K +E ++ DF+S    Y  AH+FF +
Sbjct:    6 IIDNVFKPLHKVGEWKVLVVDHLSMRMVSSCLKMHDLLEVGITIVENVHIQREPLNRLEAIYLITPDEKVLEEVIEDFDSFFLTYKSAHIFFTE 99

[Close]

BLAST of SMED30020834 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 86.2705 bits (212), Expect = 1.962e-17
Identity = 40/99 (40.40%), Postives = 65/99 (65.66%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1604
            +  +I + V     E+  W++LV+D+ S ++++   KM D+  +G+T+V+D+TKRREP+P +EAIYLI+P  + +E ++ DF     P Y  AHVFF D
Sbjct:   10 VGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 86.2705 bits (212), Expect = 2.041e-17
Identity = 40/99 (40.40%), Postives = 65/99 (65.66%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1604
            +  +I + V     E+  W++LV+D+ S ++++   KM D+  +G+T+V+D+TKRREP+P +EAIYLI+P  + +E ++ DF     P Y  AHVFF D
Sbjct:   10 VGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 86.2705 bits (212), Expect = 2.206e-17
Identity = 40/99 (40.40%), Postives = 65/99 (65.66%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1604
            +  +I + V     E+  W++LV+D+ S ++++   KM D+  +G+T+V+D+TKRREP+P +EAIYLI+P  + +E ++ DF     P Y  AHVFF D
Sbjct:   10 VGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 85.8853 bits (211), Expect = 2.928e-17
Identity = 40/99 (40.40%), Postives = 65/99 (65.66%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1604
            +  +I + V     E+  W++LV+D+ S ++++   KM D+  +G+T+V+D+TKRREP+P +EAIYLI+P  + +E ++ DF     P Y  AHVFF D
Sbjct:   10 VGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTD 108

[Close]

BLAST of SMED30020834 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 85.8853 bits (211), Expect = 2.966e-17
Identity = 40/99 (40.40%), Postives = 65/99 (65.66%), Query Frame = -3

Query: 1317 IHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVP-LEAIYLISPEKKKIELMLRDFE--STPVYSKAHVFFVD 1604
            +  +I + V     E+  W++LV+D+ S ++++   KM D+  +G+T+V+D+TKRREP+P +EAIYLI+P  + +E ++ DF     P Y  AHVFF D
Sbjct:   10 VGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTD 108

[Close]

BLAST of SMED30020834 vs. Planmine SMEST
Match: SMESG000060160.1 (SMESG000060160.1)

HSP 1 Score: 199.519 bits (506), Expect = 5.224e-56
Identity = 96/97 (98.97%), Postives = 96/97 (98.97%), Query Frame = -3

Query: 1317 NIHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVPLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVD 1607
            NIHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDL KRREPVPLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVD
Sbjct:    6 NIHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLAKRREPVPLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVD 102

[Close]

BLAST of SMED30020834 vs. Planmine SMEST
Match: SMESG000060160.1 (SMESG000060160.1)

HSP 1 Score: 105.916 bits (263), Expect = 9.318e-24
Identity = 50/53 (94.34%), Postives = 51/53 (96.23%), Query Frame = -3

Query: 1308 TLVDDLTKRREPVPLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVDGEA 1466
            + VDDL KRREPVPLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVDGEA
Sbjct:  109 SFVDDLAKRREPVPLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFFVDGEA 161

[Close]

BLAST of SMED30020834 vs. Planmine SMEST
Match: SMESG000013817.1 (SMESG000013817.1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.468e-18
Identity = 41/99 (41.41%), Postives = 69/99 (69.70%), Query Frame = -3

Query: 1314 NIHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDKGVTLVDDLTKRREPVPLEAIYLISPEKKKIELMLRDF-ESTPVYSKAHVFFVDG 1607
            ++ ++I   V   + +E  W++L++D+ ST++I+   KMHD+ ++G+TLV+D+ K+RE +PLEAIYLI+P +K I+ ++ DF  S   Y  AHVFF + 
Sbjct:    5 SVAKKILDDVIRTIKKEGEWKVLIVDKLSTRMISSCCKMHDIMNEGITLVEDINKKREILPLEAIYLITPTEKSIKSLMADFTGSKNQYKAAHVFFTEA 103

The following BLAST results are available for this feature:

BLAST of SMED30020834 vs. Ensembl Human
BLAST of SMED30020834 vs. Ensembl Celegans
BLAST of SMED30020834 vs. Ensembl Fly
BLAST of SMED30020834 vs. Ensembl Zebrafish
BLAST of SMED30020834 vs. Ensembl Xenopus
BLAST of SMED30020834 vs. Ensembl Mouse
BLAST of SMED30020834 vs. UniProt/SwissProt
BLAST of SMED30020834 vs. TrEMBL
BLAST of SMED30020834 vs. Ensembl Cavefish
BLAST of SMED30020834 vs. Ensembl Sea Lamprey
BLAST of SMED30020834 vs. Ensembl Yeast
BLAST of SMED30020834 vs. Ensembl Nematostella
BLAST of SMED30020834 vs. Ensembl Medaka
BLAST of SMED30020834 vs. Planmine SMEST

BLAST of SMED30020834 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
STXBP2	6.548e-20	45.68	syntaxin binding protein 2 [Source:HGNC Symbol;Acc...	[more]
STXBP2	6.010e-19	40.40	syntaxin binding protein 2 [Source:HGNC Symbol;Acc...	[more]
STXBP2	1.135e-18	40.40	syntaxin binding protein 2 [Source:HGNC Symbol;Acc...	[more]
STXBP1	2.898e-18	43.90	syntaxin binding protein 1 [Source:HGNC Symbol;Acc...	[more]
STXBP1	7.830e-18	43.90	syntaxin binding protein 1 [Source:HGNC Symbol;Acc...	[more]

BLAST of SMED30020834 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4


Match Name	E-value	Identity	Description
unc-18	2.677e-15	40.24	Putative acetylcholine regulator unc-18 [Source:U...	[more]
T07A9.10	4.758e-12	38.82	pep chromosome:WBcel235:IV:390512:393158:1 gene:WB...	[more]
sly-1	8.095e-6	36.00	SLY1 homolog [Source:UniProtKB/TrEMBL;Acc:Q20364]	[more]
sly-1	9.358e-6	36.00	SLY1 homolog [Source:UniProtKB/TrEMBL;Acc:Q20364]	[more]

BLAST of SMED30020834 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Rop	7.530e-16	43.21	gene:FBgn0004574 transcript:FBtr0310632	[more]
Rop	7.530e-16	43.21	gene:FBgn0004574 transcript:FBtr0073263	[more]

BLAST of SMED30020834 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
stxbp2	4.094e-17	43.30	syntaxin binding protein 2 [Source:ZFIN;Acc:ZDB-GE...	[more]
stxbp1b	3.417e-16	37.00	syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-G...	[more]
stxbp1b	3.417e-16	37.00	syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-G...	[more]
stxbp1a	1.850e-14	43.21	syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-G...	[more]
stxbp1a	2.190e-14	42.68	syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-G...	[more]

BLAST of SMED30020834 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
krt8.1	1.064e-16	38.38	keratin 8, gene 1 [Source:Xenbase;Acc:XB-GENE-8769...	[more]
stxbp1	1.212e-15	43.90	syntaxin binding protein 1 [Source:NCBI gene;Acc:7...	[more]
stxbp1	1.266e-15	38.00	syntaxin binding protein 1 [Source:NCBI gene;Acc:7...	[more]
stxbp1	1.338e-15	43.90	syntaxin binding protein 1 [Source:NCBI gene;Acc:7...	[more]
stxbp1	1.379e-15	43.90	syntaxin binding protein 1 [Source:NCBI gene;Acc:7...	[more]

BLAST of SMED30020834 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Stxbp2	1.731e-18	40.40	syntaxin binding protein 2 [Source:MGI Symbol;Acc:...	[more]
Stxbp2	1.774e-18	40.40	syntaxin binding protein 2 [Source:MGI Symbol;Acc:...	[more]
Stxbp1	9.406e-16	43.90	syntaxin binding protein 1 [Source:MGI Symbol;Acc:...	[more]
Stxbp3	9.876e-16	38.38	syntaxin binding protein 3 [Source:MGI Symbol;Acc:...	[more]
Stxbp1	1.091e-15	43.90	syntaxin binding protein 1 [Source:MGI Symbol;Acc:...	[more]

BLAST of SMED30020834 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q29268\|STXB2_PIG	1.060e-18	39.80	Syntaxin-binding protein 2 (Fragment) OS=Sus scrof...	[more]
sp\|Q15833\|STXB2_HUMAN	5.452e-18	40.40	Syntaxin-binding protein 2 OS=Homo sapiens OX=9606...	[more]
sp\|Q64324\|STXB2_MOUSE	1.241e-17	40.40	Syntaxin-binding protein 2 OS=Mus musculus OX=1009...	[more]
sp\|Q62753\|STXB2_RAT	2.328e-17	41.67	Syntaxin-binding protein 2 OS=Rattus norvegicus OX...	[more]
sp\|Q28288\|STXB2_CANLF	6.243e-17	39.39	Syntaxin-binding protein 2 OS=Canis lupus familiar...	[more]

BLAST of SMED30020834 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2D4FWY2	8.224e-19	43.40	Uncharacterized protein (Fragment) OS=Micrurus cor...	[more]
A0A2D4FWZ1	1.977e-18	43.40	Uncharacterized protein (Fragment) OS=Micrurus cor...	[more]
A0A5J4NQ93	2.143e-18	45.26	Syntaxin-binding protein 1 OS=Paragonimus westerma...	[more]
A0A1S8X7V6	3.549e-17	46.59	Sec1 family protein (Fragment) OS=Opisthorchis viv...	[more]
A0A2D4JZP0	3.594e-17	44.90	Uncharacterized protein (Fragment) OS=Micrurus par...	[more]

BLAST of SMED30020834 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
stxbp2	1.091e-14	37.76	syntaxin binding protein 2 [Source:NCBI gene;Acc:1...	[more]
stxbp1a	1.266e-14	43.21	syntaxin binding protein 1 [Source:NCBI gene;Acc:1...	[more]
stxbp1a	1.453e-14	42.68	syntaxin binding protein 1 [Source:NCBI gene;Acc:1...	[more]
stxbp3	2.407e-14	39.08	syntaxin binding protein 3 [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30020834 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
stxbp1a	5.314e-17	37.37	syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-G...	[more]

BLAST of SMED30020834 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
SLY1	1.003e-9	34.44	Hydrophilic protein involved in ER/Golgi vesicle t...	[more]

BLAST of SMED30020834 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO43368	3.933e-17	50.59	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO43369	1.101e-15	42.55	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30020834 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
stxbp1b	1.962e-17	40.40	syntaxin-binding protein 1 [Source:NCBI gene;Acc:1...	[more]
stxbp1b	2.041e-17	40.40	syntaxin-binding protein 1 [Source:NCBI gene;Acc:1...	[more]
stxbp1b	2.206e-17	40.40	syntaxin-binding protein 1 [Source:NCBI gene;Acc:1...	[more]
stxbp1b	2.928e-17	40.40	syntaxin-binding protein 1 [Source:NCBI gene;Acc:1...	[more]
stxbp1b	2.966e-17	40.40	syntaxin-binding protein 1 [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30020834 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000060160.1	5.224e-56	98.97	SMESG000060160.1	[more]
SMESG000060160.1	9.318e-24	94.34	SMESG000060160.1	[more]
SMESG000013817.1	2.468e-18	41.41	SMESG000013817.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30020834 ID=SMED30020834|Name=SMED30020834|organism=Schmidtea mediterranea sexual|type=transcript|length=1687bp
CTACAAATGGTGAAATTTAAATTCAATATAGAAATAATATAACTAAATGT
TATGCGAAACTTAAAATCCAACTTAAATAAAAATGAAATATATTTCAACG
AAATGGTCGTCATTTCAGTTATCAGTGTTAAAACTCAATAAATCAAATAA
ATACCATCAATAAATTAGTTATTGATTGGGTATTAATGGAAGAGGATTGA
CAGACAGTTTTCAATATTTTACAGGAAATCTTTTCTCTATAATTGTCAAA
ATTTCTAAGTTAAATTTATGTTGGAGATTCACTTGTAGATTTTTCTATGT
TGAATAATATTACTCAAACTCTTCATAACACTCTTTACTTGAGCACTAGT
GTAAAGAGTTTCGTGAGTCTTCCATTGTATGTGACAGTTTCACTCTACGG
ATACTCAAACAAAAAATATTAATAATAAATATTTGTTCTTTAATGTATTT
GAAACTATTCTCTTTCAAATTTGAATGATTAAAATATTTGAAACCTTTCA
AAATGATGGGCAATTTTCTAACGGAAACTCGTGGTTAAACAATGATGATA
GATAAGAATCAAAGATATATAAGAGCGATTAATTTTATTTAGATTTGTCA
TTATTAGTCGAAGAAATTACGTTACCATAAAGAAAAATAAAAAAGATAAA
CCAAAACTAAGTTCAAAACACAGCTACAAGCTACTTACGGATCAACCCTT
ATGAAGATTTACCTTATTTTGAAATATTAAAAAAAGAAAATACATTCCTT
ATGTGATAACTGGATCTTAATTGTTGTCAATTATGTATTTTAAATAAAGT
TTTAATAATGCTTGTTATTAGTTTTATATAGTGAAAGATGAAATGACTTA
CTGGCCGAAAGATTTATGAATTCAACAGCGCCTACTTCCAAATTAATTGT
AATATATCTAAATGTAAATGACTTTTTAATCAAATTTATAATCTCACTTA
AATACCAAAATAGCCTTGAACATCTAGAGAAAAACCTTTATAATTTAGGG
TCTTTATTAATTTGTAAAAGCGGTAAAAGTATTTCCCAAAGTTTTGTTTA
AGTAAATGGACTGCTGTGGACTCTTATAATTCAATGAAATAGTCAATTAC
CGATCCAGCTCACCCAATATCAATTATCAATACAATAAGAATTTGGTCGG
CATCAAAAGATGTTTGGCGCCCACGTTTGACGGATCAGCACTATGAACAA
GGTCCAACGACAAGTATTAGAGATCAATTATTCTAACACAGTCAAAAATC
GTCAATATTATCATAATAAAATAAACAAATTTGTGGCTAATAAATCAACA
AATCACCAGCCTCACCGTCAACAAAAAACACATGTGCCTTGCTGTAGACC
GGGGTTGATTCAAAATCACGAAGCATCAATTCAATTTTCTTTTTTTCTGG
GGATATTAAATAAATGGCTTCCAGCGGCACGGGTTCTCGTCGTTTGGTTA
AGTCATCGACAAGAGTTACTCCTTTATCGAATAGATCGTGCATTTTAAGG
ATTTTTGCAATAATTTGCGTACTATATTCATCAAGAACGAGAATCCTCCA
GATGTTTTCTTCGTATAGAGGATCAAACACTTTCGAACATATCTCTCTAT
GAATATTTAATTTCAGAGACATTCATAAAATAGAAAAACTTTTAAATAAA
AGTTAATGTAATTTAATGAACTAAAACAATATACAAA

protein sequence of SMED30020834-orf-1

>SMED30020834-orf-1 ID=SMED30020834-orf-1|Name=SMED30020834-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=112bp
MSLKLNIHREICSKVFDPLYEENIWRILVLDEYSTQIIAKILKMHDLFDK
GVTLVDDLTKRREPVPLEAIYLISPEKKKIELMLRDFESTPVYSKAHVFF
VDGEAGDLLIY*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000099	neuron

Vocabulary: **INTERPRO**
Term	Definition
IPR043154	Sec-1-like_dom1
IPR036045	Sec1-like_sf
IPR001619	Sec1-like

Vocabulary: **biological process**
Term	Definition
GO:0006904	vesicle docking involved in exocytosis
GO:0016192	vesicle-mediated transport

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001619	Sec1-like protein	PFAM	PF00995	Sec1	coord: 27..102 e-value: 5.9E-15 score: 55.5
IPR001619	Sec1-like protein	PANTHER	PTHR11679	VESICLE PROTEIN SORTING-ASSOCIATED	coord: 7..104
IPR043154	Sec1-like, domain 1	GENE3D	G3DSA:3.40.50.2060		coord: 1..109 e-value: 4.7E-32 score: 112.3
None	No IPR available	PANTHER	PTHR11679:SF27	SYNTAXIN-BINDING PROTEIN 2	coord: 7..104
IPR036045	Sec1-like superfamily	SUPERFAMILY	SSF56815	Sec1/munc18-like (SM) proteins	coord: 15..102