SMED30020814
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020814 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14
Homology
BLAST of SMED30020814 vs. Planmine SMEST
Match: SMESG000013157.1 (SMESG000013157.1) HSP 1 Score: 311.612 bits (797), Expect = 3.160e-98 Identity = 149/175 (85.14%), Postives = 164/175 (93.71%), Query Frame = 3 Query: 1851 NSWIDASRVIARFPAHSAARCRQAMWDAGFSISPVRETRGGIQGDSDEVDLAYVAIKREPKRNPAREMRGQAEYLMARLERHQAETNRQVEQARQYLQRVNEDIAAGRIPSTDGYAPPYNVSYELDDPRLQRRMPEMRVAVPRQILPKAMRNEDEVTELHREHSYARERYIQKRK 2375 +SWIDASRVIARFPAHSAARCRQAMWDAGFSI PVRETRGG QGDS+EVDL+YVAIKREP R+PAREMRGQAEYL+ARLERHQAETNRQVEQARQYL+RVNEDIAAGRIPSTDGYAP YNVS+ELDDPRLQR+M EMRVAVPR ILPK MRNE+++ E REHSYARER +++R+ Sbjct: 90 DSWIDASRVIARFPAHSAARCRQAMWDAGFSIFPVRETRGGTQGDSNEVDLSYVAIKREPGRSPAREMRGQAEYLLARLERHQAETNRQVEQARQYLRRVNEDIAAGRIPSTDGYAPQYNVSFELDDPRLQRQMSEMRVAVPRHILPKEMRNENDIAEPQREHSYARERNLKRRR 264
BLAST of SMED30020814 vs. Planmine SMEST
Match: SMESG000063851.1 (SMESG000063851.1) HSP 1 Score: 305.064 bits (780), Expect = 7.739e-97 Identity = 150/170 (88.24%), Postives = 158/170 (92.94%), Query Frame = 3 Query: 1866 ASRVIARFPAHSAARCRQAMWDAGFSISPVRETRGGIQGDSDEVDLAYVAIKREPKRNPAREMRGQAEYLMARLERHQAETNRQVEQARQYLQRVNEDIAAGRIPSTDGYAPPYNVSYELDDPRLQRRMPEMRVAVPRQILPKAMRNEDEVTELHREHSYARERYIQKRK 2375 ASRVIARFP HSAARCRQAMWDAGFSISPVRE RGG QGDSDEV L YVAIKRE R+PARE+RGQAE+L+ARLE HQAETNRQVEQARQYLQRVNEDIAAG IPSTDGYAP YNVSYEL+DPRLQRRMPEMRVAVPR ILPK MRNEDE+TELHREHSYARERYI+KR+ Sbjct: 18 ASRVIARFPTHSAARCRQAMWDAGFSISPVREARGGTQGDSDEVYLPYVAIKRESGRSPAREIRGQAEHLLARLEHHQAETNRQVEQARQYLQRVNEDIAAGWIPSTDGYAPQYNVSYELNDPRLQRRMPEMRVAVPRHILPKEMRNEDEITELHREHSYARERYIKKRR 187
BLAST of SMED30020814 vs. Planmine SMEST
Match: SMESG000036578.1 (SMESG000036578.1) HSP 1 Score: 250.751 bits (639), Expect = 2.340e-77 Identity = 125/147 (85.03%), Postives = 132/147 (89.80%), Query Frame = 3 Query: 2589 MTIRLISEMNSPQSSMLQRELDKAQAANRNLHHQLIQLTREIQ*IKGTWIEPKRAKIVYHRMEAAQKGWAGERQLTQNLKTQIKGLETALSASQEGAAVTYPLVFTLAQLAYRDTINPTAFKDNPSRYRPGRAEGAKRRRLKETGGL 3029 MT RLISE NSPQ SMLQ ELDKAQAAN++LH QLIQLTREIQ IKGTWIEPKRAK+VY RMEAAQK WAG+RQLTQNLKTQIKGL+TALSASQEGAAVTYPLVF AQLAYRDTINPT KD PSR+RPG+AE AKRRRLKET GL Sbjct: 1 MTNRLISERNSPQFSMLQLELDKAQAANQDLHQQLIQLTREIQQIKGTWIEPKRAKVVYQRMEAAQKEWAGKRQLTQNLKTQIKGLKTALSASQEGAAVTYPLVFAPAQLAYRDTINPTTSKDKPSRHRPGKAECAKRRRLKETDGL 147
BLAST of SMED30020814 vs. Planmine SMEST
Match: SMESG000028747.1 (SMESG000028747.1) HSP 1 Score: 243.432 bits (620), Expect = 7.221e-74 Identity = 113/141 (80.14%), Postives = 125/141 (88.65%), Query Frame = 3 Query: 1803 MKHKFPLFPDIFFLLENSWIDASRVIARFPAHSAARCRQAMWDAGFSISPVRETRGGIQGDSDEVDLAYVAIKREPKRNPAREMRGQAEYLMARLERHQAETNRQVEQARQYLQRVNEDIAAGRIPSTDGYAPPYNVSYEL 2225 MKH F L P++ FLLENSWIDASRVIARFPAHSAARCRQAMWDA FSISPVRE R Q +SDEVDL YV IKREP RNPAREMRGQAEYL+ARLE+HQ ETN+QVE+ RQYL+++N DIAAG+IPSTDGYAPPYN+SYEL Sbjct: 1 MKHNFQLLPNVSFLLENSWIDASRVIARFPAHSAARCRQAMWDARFSISPVREARSDKQRNSDEVDLPYVVIKREPGRNPAREMRGQAEYLLARLEKHQKETNKQVEKPRQYLRQINADIAAGKIPSTDGYAPPYNISYEL 141 HSP 2 Score: 63.1586 bits (152), Expect = 8.682e-11 Identity = 32/55 (58.18%), Postives = 38/55 (69.09%), Query Frame = 3 Query: 2841 GLETALSASQEGAAVTYPLVFTLAQLAYRDTINPTAFKDNPSRYRPGRAEGAKRR 3005 GLE ALSA QE A+VTYPLVF AQLAYR+ + P + RPG++E AKRR Sbjct: 142 GLEVALSACQEEASVTYPLVFAPAQLAYREAAATSTTTSRPIKRRPGKSERAKRR 196
BLAST of SMED30020814 vs. Planmine SMEST
Match: SMESG000065931.1 (SMESG000065931.1) HSP 1 Score: 242.662 bits (618), Expect = 8.371e-74 Identity = 117/179 (65.36%), Postives = 145/179 (81.01%), Query Frame = 3 Query: 1833 IFFLLENSWIDASRVIARFPAHSAARCRQAMWDAGFSISPVRETRGGIQGDSDEVDLAYVAIKREPKRNPAREMRGQAEYLMARLERHQAETNRQVEQARQYLQRVNEDIAAGRIPSTDGYAPPYNVSYELDDPRLQRRMPEMRVAVPRQILPKAMRNEDEVTELHREHSYARERYIQK 2369 I + LE+SWIDA RVIARFPAH+AARCRQAMWDAGFSIS VRETR G QG S++V+L YVAIKREP N ARE+RGQAE+L+ RLE HQ ET RQ+ +AR+YL +VN+DIA GRIPSTDGYAP YNVS+ELDDP L R+ M V +PR++LP+ +RN +E ++ ++HS+AR R Q+ Sbjct: 7 IHYNLEDSWIDAPRVIARFPAHAAARCRQAMWDAGFSISTVRETRFGNQGSSEDVELPYVAIKREPGGNLAREIRGQAEFLLGRLEEHQKETERQIIKARRYLAQVNKDIAEGRIPSTDGYAPSYNVSFELDDPSLCNRLETMNVILPRRVLPRELRNVEENSDSRQDHSFARNRQSQR 185 The following BLAST results are available for this feature:
BLAST of SMED30020814 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30020814 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30020814 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30020814 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30020814 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020814 ID=SMED30020814|Name=SMED30020814|organism=Schmidtea mediterranea sexual|type=transcript|length=3218bpback to top protein sequence of SMED30020814-orf-1 >SMED30020814-orf-1 ID=SMED30020814-orf-1|Name=SMED30020814-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=215bp MKLTNTKSEDYQSAKMKHKFPLFPDIFFLLENSWIDASRVIARFPAHSAAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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