SMED30020792

Overview
NameSMED30020792
Smed IDSMED30020792
Length (bp)751
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020792 (SMED30020792) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020792 (SMED30020792) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30020792

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30020792h1SMcG0011840 SmedASXL_007303SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30020792h1SMcG0011840 dd_Smed_v6_22514_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30020792 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.011753:785..3378 -3484
Homology
BLAST of SMED30020792 vs. Planmine SMEST
Match: SMESG000016092.1 (SMESG000016092.1)

HSP 1 Score: 420.624 bits (1080), Expect = 2.152e-148
Identity = 237/245 (96.73%), Postives = 239/245 (97.55%), Query Frame = 3
Query:    3 MCLISYRCLSDFEGARCENPTIPKNRMKVKFQNPMFTTKTPCSYGCNLTGNHIFFYAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXANVETICSNKNRERINRDNSKRSSDKKAEKQRTIHKQNHNKSEYLVQQFSPENNKICQDCRIYRSSRTNPGVSHMIKCYSSYITDNPSLETLDDNIELLTDNNSSNKCPTSVINYTQQNICRTQEIRIHPCIYVDSPQFLPICTIESREYMDDQENSEN 737
            MCL    CLSDFEGARCENPTIPKNRMKVKFQNPMFTTKTPCSYGCNLTGNHIFFYAVFGFLMLILGLFLAIFLLQRKRRLQLLNRANVETICSNKNRE+INRDNSKRSSDKKAEK RTIHKQNHNKSEYLVQQF+PEN KICQDCRIYRSSRTNPGVSHMIKCYSSYITDNPSLETLDDNIELLTDNNSSNKCPTSVINYTQQNICRTQEIRIHPCIYVDSPQFLPICTIESREYMDDQENSEN
Sbjct:  170 MCL----CLSDFEGARCENPTIPKNRMKVKFQNPMFTTKTPCSYGCNLTGNHIFFYAVFGFLMLILGLFLAIFLLQRKRRLQLLNRANVETICSNKNREQINRDNSKRSSDKKAEKLRTIHKQNHNKSEYLVQQFTPENKKICQDCRIYRSSRTNPGVSHMIKCYSSYITDNPSLETLDDNIELLTDNNSSNKCPTSVINYTQQNICRTQEIRIHPCIYVDSPQFLPICTIESREYMDDQENSEN 410          
BLAST of SMED30020792 vs. Planmine SMEST
Match: SMESG000016092.1 (SMESG000016092.1)

HSP 1 Score: 421.009 bits (1081), Expect = 2.273e-148
Identity = 237/245 (96.73%), Postives = 239/245 (97.55%), Query Frame = 3
Query:    3 MCLISYRCLSDFEGARCENPTIPKNRMKVKFQNPMFTTKTPCSYGCNLTGNHIFFYAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXANVETICSNKNRERINRDNSKRSSDKKAEKQRTIHKQNHNKSEYLVQQFSPENNKICQDCRIYRSSRTNPGVSHMIKCYSSYITDNPSLETLDDNIELLTDNNSSNKCPTSVINYTQQNICRTQEIRIHPCIYVDSPQFLPICTIESREYMDDQENSEN 737
            MCL    CLSDFEGARCENPTIPKNRMKVKFQNPMFTTKTPCSYGCNLTGNHIFFYAVFGFLMLILGLFLAIFLLQRKRRLQLLNRANVETICSNKNRE+INRDNSKRSSDKKAEK RTIHKQNHNKSEYLVQQF+PEN KICQDCRIYRSSRTNPGVSHMIKCYSSYITDNPSLETLDDNIELLTDNNSSNKCPTSVINYTQQNICRTQEIRIHPCIYVDSPQFLPICTIESREYMDDQENSEN
Sbjct:  180 MCL----CLSDFEGARCENPTIPKNRMKVKFQNPMFTTKTPCSYGCNLTGNHIFFYAVFGFLMLILGLFLAIFLLQRKRRLQLLNRANVETICSNKNREQINRDNSKRSSDKKAEKLRTIHKQNHNKSEYLVQQFTPENKKICQDCRIYRSSRTNPGVSHMIKCYSSYITDNPSLETLDDNIELLTDNNSSNKCPTSVINYTQQNICRTQEIRIHPCIYVDSPQFLPICTIESREYMDDQENSEN 420          
The following BLAST results are available for this feature:
BLAST of SMED30020792 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020792 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000016092.12.152e-14896.73SMESG000016092.1[more]
SMESG000016092.12.273e-14896.73SMESG000016092.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020792 ID=SMED30020792|Name=SMED30020792|organism=Schmidtea mediterranea sexual|type=transcript|length=751bp
TTATGTGTCTTATTTCTTATAGATGTTTATCCGATTTCGAAGGTGCTCGA
TGTGAAAATCCGACAATTCCCAAAAATCGAATGAAAGTAAAATTTCAAAA
TCCCATGTTCACCACCAAAACTCCATGTTCATACGGCTGTAACTTGACGG
GTAATCACATATTCTTTTATGCTGTGTTTGGATTTTTAATGCTGATTCTC
GGATTATTTCTAGCAATTTTTCTATTACAACGAAAAAGACGTCTTCAGTT
GTTAAACAGAGCAAATGTCGAGACAATTTGTTCAAATAAAAATAGAGAAC
GAATCAATAGGGACAATTCCAAAAGATCATCGGACAAGAAAGCTGAAAAA
CAACGAACCATTCATAAGCAAAATCATAATAAGTCCGAATATCTAGTGCA
ACAATTTTCTCCGGAAAACAACAAAATATGTCAAGATTGTAGAATATATC
GAAGCAGCCGAACGAATCCAGGAGTGTCCCATATGATAAAATGTTATTCA
AGTTATATCACAGATAATCCCAGCCTTGAAACTCTTGATGATAATATTGA
ATTATTAACTGATAATAACTCCTCAAACAAATGTCCAACATCAGTCATTA
ATTATACTCAACAGAATATTTGTAGAACACAAGAAATTAGGATCCATCCT
TGCATTTACGTTGATAGTCCACAATTTTTACCTATTTGCACAATTGAAAG
TCGAGAATATATGGACGATCAGGAAAATAGTGAAAACTGATTATCAAATA
A
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protein sequence of SMED30020792-orf-1

>SMED30020792-orf-1 ID=SMED30020792-orf-1|Name=SMED30020792-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=246bp
MCLISYRCLSDFEGARCENPTIPKNRMKVKFQNPMFTTKTPCSYGCNLTG
NHIFFYAVFGFLMLILGLFLAIFLLQRKRRLQLLNRANVETICSNKNRER
INRDNSKRSSDKKAEKQRTIHKQNHNKSEYLVQQFSPENNKICQDCRIYR
SSRTNPGVSHMIKCYSSYITDNPSLETLDDNIELLTDNNSSNKCPTSVIN
YTQQNICRTQEIRIHPCIYVDSPQFLPICTIESREYMDDQENSEN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000012neural progenitor cell
PLANA:0000028Category 2 cell
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 50..72