Phosphodiesterase

Overview
NamePhosphodiesterase
Smed IDSMED30020779
Length (bp)347
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Phosphodiesterase (SMED30020779) t-SNE clustered cells

Violin plots show distribution of expression levels for Phosphodiesterase (SMED30020779) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phosphodiesterase (SMED30020779) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phosphodiesterase (SMED30020779) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020779

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30020779SMESG000019038.1 dd_Smed_v4_8595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30020779SMESG000019038.1 dd_Smed_v4_8595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30020779SMESG000019038.1 dd_Smed_v4_8595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30020779SMESG000019038.1 dd_Smed_v4_8595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Phosphodiesterase vs. Ensembl Human
Match: PDE5A (phosphodiesterase 5A [Source:HGNC Symbol;Acc:HGNC:8784])

HSP 1 Score: 51.9878 bits (123), Expect = 2.741e-8
Identity = 32/96 (33.33%), Postives = 54/96 (56.25%), Query Frame = 3 
Query:   60 VAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            V  WL+++ +FT +YF++ ATR M+ +W + R  +    +  +       S  L ++     S   TP RKIS+ EFD   LRPI+  +S+G+++F
Sbjct:   30 VEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDR-PLRPIVVKDSEGTVSF 124
[Close]
BLAST of Phosphodiesterase vs. Ensembl Fly
Match: Pde11 (gene:FBgn0085370 transcript:FBtr0112544)

HSP 1 Score: 63.1586 bits (152), Expect = 1.334e-12
Identity = 41/103 (39.81%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:   42 DPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            DP   ++  WL+EN EF ++YFI+ ATR  +++W      S+ +   DV      +      +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  193 DPEHARMEAWLDENQEFVQDYFIRKATRQTVDAWLVSHATSAGN---DVVSSTSPTHANGQTSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 291
[Close]
BLAST of Phosphodiesterase vs. Ensembl Fly
Match: Pde11 (gene:FBgn0085370 transcript:FBtr0343830)

HSP 1 Score: 63.1586 bits (152), Expect = 1.334e-12
Identity = 41/103 (39.81%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:   42 DPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            DP   ++  WL+EN EF ++YFI+ ATR  +++W      S+ +   DV      +      +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  193 DPEHARMEAWLDENQEFVQDYFIRKATRQTVDAWLVSHATSAGN---DVVSSTSPTHANGQTSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 291
[Close]
BLAST of Phosphodiesterase vs. Ensembl Fly
Match: Pde11 (gene:FBgn0085370 transcript:FBtr0343831)

HSP 1 Score: 63.1586 bits (152), Expect = 1.497e-12
Identity = 41/103 (39.81%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:   42 DPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            DP   ++  WL+EN EF ++YFI+ ATR  +++W      S+ +   DV      +      +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  149 DPEHARMEAWLDENQEFVQDYFIRKATRQTVDAWLVSHATSAGN---DVVSSTSPTHANGQTSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 247
[Close]
BLAST of Phosphodiesterase vs. Ensembl Fly
Match: Pde11 (gene:FBgn0085370 transcript:FBtr0112545)

HSP 1 Score: 63.1586 bits (152), Expect = 1.497e-12
Identity = 41/103 (39.81%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:   42 DPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            DP   ++  WL+EN EF ++YFI+ ATR  +++W      S+ +   DV      +      +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  149 DPEHARMEAWLDENQEFVQDYFIRKATRQTVDAWLVSHATSAGN---DVVSSTSPTHANGQTSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 247
[Close]
BLAST of Phosphodiesterase vs. Ensembl Fly
Match: Pde11 (gene:FBgn0085370 transcript:FBtr0303163)

HSP 1 Score: 62.7734 bits (151), Expect = 1.712e-12
Identity = 41/103 (39.81%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:   42 DPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            DP   ++  WL+EN EF ++YFI+ ATR  +++W      S+ +   DV      +      +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  149 DPEHARMEAWLDENQEFVQDYFIRKATRQTVDAWLVSHATSAGN---DVVSSTSPTHANGQTSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 247
[Close]
BLAST of Phosphodiesterase vs. Ensembl Xenopus
Match: pde5a (phosphodiesterase 5A [Source:NCBI gene;Acc:780273])

HSP 1 Score: 46.595 bits (109), Expect = 1.924e-6
Identity = 34/106 (32.08%), Postives = 57/106 (53.77%), Query Frame = 3 
Query:   54 EKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKS--------GSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            E +  WL+++ +FT +YF++ ATR M+ +W + R  +              +S +  +N+S+S         +   TPVRKIS+ EFD   LRPI+  +S G++ F
Sbjct:   13 EGIESWLDDHWDFTHSYFVRKATREMVNAWFAERVHTIP------------ASKEQGKNNSESNPQVCYSENTTQGTPVRKISASEFDR-PLRPIVVKDSVGTLKF 105
[Close]
BLAST of Phosphodiesterase vs. Ensembl Xenopus
Match: pde5a (phosphodiesterase 5A [Source:NCBI gene;Acc:780273])

HSP 1 Score: 46.2098 bits (108), Expect = 1.989e-6
Identity = 34/106 (32.08%), Postives = 57/106 (53.77%), Query Frame = 3 
Query:   54 EKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKS--------GSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            E +  WL+++ +FT +YF++ ATR M+ +W + R  +              +S +  +N+S+S         +   TPVRKIS+ EFD   LRPI+  +S G++ F
Sbjct:   13 EGIESWLDDHWDFTHSYFVRKATREMVNAWFAERVHTIP------------ASKEQGKNNSESNPQVCYSENTTQGTPVRKISASEFDR-PLRPIVVKDSVGTLKF 105
[Close]
BLAST of Phosphodiesterase vs. Ensembl Xenopus
Match: pde5a (phosphodiesterase 5A [Source:NCBI gene;Acc:780273])

HSP 1 Score: 45.8246 bits (107), Expect = 3.184e-6
Identity = 36/98 (36.73%), Postives = 57/98 (58.16%), Query Frame = 3 
Query:   54 EKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            E +  WL+++ +FT +YF++ ATR M+ +W + R  +  +S+   +  N   SN     S  +  G  TPVRKIS+ EFD   LRPI+  +S G++ F
Sbjct:   16 EGIESWLDDHWDFTHSYFVRKATREMVNAWFAERVHTIPASKEQGK--NNSESNPQVCYSENTTQG--TPVRKISASEFDR-PLRPIVVKDSVGTLKF 108
[Close]
BLAST of Phosphodiesterase vs. Ensembl Xenopus
Match: pde5a (phosphodiesterase 5A [Source:NCBI gene;Acc:780273])

HSP 1 Score: 45.8246 bits (107), Expect = 3.214e-6
Identity = 36/95 (37.89%), Postives = 56/95 (58.95%), Query Frame = 3 
Query:   63 AIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            A WL+++ +FT +YF++ ATR M+ +W + R  +  +S+   +  N   SN     S  +  G  TPVRKIS+ EFD   LRPI+  +S G++ F
Sbjct:   21 ASWLDDHWDFTHSYFVRKATREMVNAWFAERVHTIPASKEQGK--NNSESNPQVCYSENTTQG--TPVRKISASEFDR-PLRPIVVKDSVGTLKF 110
[Close]
BLAST of Phosphodiesterase vs. Ensembl Mouse
Match: Pde5a (phosphodiesterase 5A, cGMP-specific [Source:MGI Symbol;Acc:MGI:2651499])

HSP 1 Score: 52.373 bits (124), Expect = 1.275e-8
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 3 
Query:   27 SSYKNDPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            S  + DP  + V  WL+++ +FT +YFI+ ATR M+ +W S R  +    +  +       S  L ++     +    P RKIS+ EFD   LRPI+  +S+G+++F
Sbjct:   11 SQQQRDP--DWVEAWLDDHRDFTFSYFIRKATRDMVNAWFSERVHNIPVCKEGIRAHTESCSCSLQQSPHADNTTPGAPARKISASEFDR-PLRPIVVKDSEGTVSF 114
[Close]
BLAST of Phosphodiesterase vs. UniProt/SwissProt
Match: sp|Q9VJ79|PDE11_DROME (Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11 OS=Drosophila melanogaster OX=7227 GN=Pde11 PE=1 SV=4)

HSP 1 Score: 63.1586 bits (152), Expect = 1.337e-11
Identity = 41/103 (39.81%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:   42 DPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            DP   ++  WL+EN EF ++YFI+ ATR  +++W      S+ +   DV      +      +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  193 DPEHARMEAWLDENQEFVQDYFIRKATRQTVDAWLVSHATSAGN---DVVSSTSPTHANGQTSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 291
[Close]
BLAST of Phosphodiesterase vs. UniProt/SwissProt
Match: sp|Q8CG03|PDE5A_MOUSE (cGMP-specific 3',5'-cyclic phosphodiesterase OS=Mus musculus OX=10090 GN=Pde5a PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 8.920e-8
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 3 
Query:   27 SSYKNDPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            S  + DP  + V  WL+++ +FT +YFI+ ATR M+ +W S R  +    +  +       S  L ++     +    P RKIS+ EFD   LRPI+  +S+G+++F
Sbjct:   11 SQQQRDP--DWVEAWLDDHRDFTFSYFIRKATRDMVNAWFSERVHNIPVCKEGIRAHTESCSCSLQQSPHADNTTPGAPARKISASEFDR-PLRPIVVKDSEGTVSF 114
[Close]
BLAST of Phosphodiesterase vs. UniProt/SwissProt
Match: sp|O76074|PDE5A_HUMAN (cGMP-specific 3',5'-cyclic phosphodiesterase OS=Homo sapiens OX=9606 GN=PDE5A PE=1 SV=2)

HSP 1 Score: 51.9878 bits (123), Expect = 1.316e-7
Identity = 32/96 (33.33%), Postives = 54/96 (56.25%), Query Frame = 3 
Query:   60 VAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            V  WL+++ +FT +YF++ ATR M+ +W + R  +    +  +       S  L ++     S   TP RKIS+ EFD   LRPI+  +S+G+++F
Sbjct:   30 VEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDR-PLRPIVVKDSEGTVSF 124
[Close]
BLAST of Phosphodiesterase vs. UniProt/SwissProt
Match: sp|O77746|PDE5A_CANLF (cGMP-specific 3',5'-cyclic phosphodiesterase OS=Canis lupus familiaris OX=9615 GN=PDE5A PE=2 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.805e-6
Identity = 32/98 (32.65%), Postives = 55/98 (56.12%), Query Frame = 3 
Query:   54 EKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            + V  WL+++ +FT +YF++ ATR M+ +W + R  +    +  +       S    ++S    S   TP RKIS+ EFD   LRPI+  +S+G+++F
Sbjct:   18 DSVEAWLDDHRDFTFSYFVKKATREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDR-PLRPIVVKDSEGTVSF 114
[Close]
BLAST of Phosphodiesterase vs. UniProt/SwissProt
Match: sp|Q28156|PDE5A_BOVIN (cGMP-specific 3',5'-cyclic phosphodiesterase OS=Bos taurus OX=9913 GN=PDE5A PE=1 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 2.415e-6
Identity = 31/98 (31.63%), Postives = 54/98 (55.10%), Query Frame = 3 
Query:   54 EKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            + V  WL+++ +FT +YF++  TR M+ +W + R  +    +  ++      S  L  +     S   TP RKIS+ EFD   LRPI+  +S+G+++F
Sbjct:   18 DSVEAWLDDHWDFTFSYFVRKGTREMVNAWFAERVHTIPVCKEGIKGHTESCSCPLQPSPRAESSVPGTPTRKISASEFDR-PLRPIVIKDSEGTVSF 114
[Close]
BLAST of Phosphodiesterase vs. TrEMBL
Match: A0A182NGQ5 (GAF domain-containing protein OS=Anopheles dirus OX=7168 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.255e-11
Identity = 41/94 (43.62%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:   69 WLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            WL+EN EF ++YFI+ ATR M++ W         ++ + V+    H +NQ   +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:    4 WLDENPEFVQDYFIRKATRNMVDGWLVSHATPVTTAANSVDSPT-HGTNQ--PSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 93
[Close]
BLAST of Phosphodiesterase vs. TrEMBL
Match: A0A4Y0BQ33 (Phosphodiesterase OS=Anopheles funestus OX=62324 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.502e-11
Identity = 42/94 (44.68%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:   69 WLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            WL+EN EF ++YFI+ ATR M++ W         ++ + V+    H SNQ   +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  115 WLDENPEFVQDYFIRKATRNMVDGWLVSHATPVTTAANSVDSPT-HGSNQ--PSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 204
[Close]
BLAST of Phosphodiesterase vs. TrEMBL
Match: A0A4Y0BL20 (Phosphodiesterase OS=Anopheles funestus OX=62324 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.730e-11
Identity = 42/94 (44.68%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:   69 WLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            WL+EN EF ++YFI+ ATR M++ W         ++ + V+    H SNQ   +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  148 WLDENPEFVQDYFIRKATRNMVDGWLVSHATPVTTAANSVDSPT-HGSNQ--PSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 237
[Close]
BLAST of Phosphodiesterase vs. TrEMBL
Match: A0A182X5V4 (GAF domain-containing protein OS=Anopheles quadriannulatus OX=34691 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.798e-11
Identity = 41/94 (43.62%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:   69 WLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            WL+EN EF ++YFI+ ATR M++ W         ++ + V+    H +NQ   +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:   51 WLDENPEFMQDYFIRKATRNMVDGWLVSHATPVTTAANSVDSPT-HGTNQ--PSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 140
[Close]
BLAST of Phosphodiesterase vs. TrEMBL
Match: A0A1Y9IUW3 (Phosphodiesterase OS=Anopheles minimus OX=112268 PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 9.217e-11
Identity = 41/94 (43.62%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:   69 WLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG-LRPILSTNSDGSITF 347
            WL+EN EF ++YFI+ ATR M++ W         ++ + V+    H +NQ   +SS+ GSG+ TPVRKIS+ EF+  G L+PI++T  DG+ TF
Sbjct:  117 WLDENPEFVQDYFIRKATRNMVDGWLVSHATPVTTAANSVDSPT-HGTNQ--PSSSRGGSGATTPVRKISAHEFERGGLLKPIVNT-IDGTPTF 206
[Close]
BLAST of Phosphodiesterase vs. Ensembl Nematostella
Match: EDO39522 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9Q1])

HSP 1 Score: 47.7506 bits (112), Expect = 1.572e-7
Identity = 31/98 (31.63%), Postives = 50/98 (51.02%), Query Frame = 3 
Query:   54 EKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            +KV  WL+E+ EF ++YF + A R +++SW                   +  S+    + +   SGS+TPVRKIS+ EF+  G+   L    DG ++F
Sbjct:   11 QKVESWLDEHPEFVQSYFARKANRSLVDSWL------------------HSRSHARTPSGTPPPSGSSTPVRKISASEFEGRGILKPLVNVVDGQLSF 90
[Close]
BLAST of Phosphodiesterase vs. Planmine SMEST
Match: SMESG000019038.1 (SMESG000019038.1)

HSP 1 Score: 178.333 bits (451), Expect = 4.650e-53
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 3 
Query:    9 NDIEYSSSYKNDPIAEKVAIWLNENSEFTKNYFIQNATRGMIEXXXXXXXXXXXXXXXDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 347
            NDIEYSSSYKNDPIAEKVAIWLNENSEFTKNYFIQNATRGMIESWKSLRKVSSKSSQSDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF
Sbjct:   60 NDIEYSSSYKNDPIAEKVAIWLNENSEFTKNYFIQNATRGMIESWKSLRKVSSKSSQSDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVGLRPILSTNSDGSITF 172
The following BLAST results are available for this feature:
BLAST of Phosphodiesterase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PDE5A2.741e-833.33phosphodiesterase 5A [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Phosphodiesterase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphodiesterase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pde111.334e-1239.81gene:FBgn0085370 transcript:FBtr0112544[more]
Pde111.334e-1239.81gene:FBgn0085370 transcript:FBtr0343830[more]
Pde111.497e-1239.81gene:FBgn0085370 transcript:FBtr0343831[more]
Pde111.497e-1239.81gene:FBgn0085370 transcript:FBtr0112545[more]
Pde111.712e-1239.81gene:FBgn0085370 transcript:FBtr0303163[more]
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BLAST of Phosphodiesterase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphodiesterase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pde5a1.924e-632.08phosphodiesterase 5A [Source:NCBI gene;Acc:780273][more]
pde5a1.989e-632.08phosphodiesterase 5A [Source:NCBI gene;Acc:780273][more]
pde5a3.184e-636.73phosphodiesterase 5A [Source:NCBI gene;Acc:780273][more]
pde5a3.214e-637.89phosphodiesterase 5A [Source:NCBI gene;Acc:780273][more]
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BLAST of Phosphodiesterase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Pde5a1.275e-832.71phosphodiesterase 5A, cGMP-specific [Source:MGI Sy... [more]
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BLAST of Phosphodiesterase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VJ79|PDE11_DROME1.337e-1139.81Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 1... [more]
sp|Q8CG03|PDE5A_MOUSE8.920e-832.71cGMP-specific 3',5'-cyclic phosphodiesterase OS=Mu... [more]
sp|O76074|PDE5A_HUMAN1.316e-733.33cGMP-specific 3',5'-cyclic phosphodiesterase OS=Ho... [more]
sp|O77746|PDE5A_CANLF1.805e-632.65cGMP-specific 3',5'-cyclic phosphodiesterase OS=Ca... [more]
sp|Q28156|PDE5A_BOVIN2.415e-631.63cGMP-specific 3',5'-cyclic phosphodiesterase OS=Bo... [more]
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BLAST of Phosphodiesterase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A182NGQ53.255e-1143.62GAF domain-containing protein OS=Anopheles dirus O... [more]
A0A4Y0BQ335.502e-1144.68Phosphodiesterase OS=Anopheles funestus OX=62324 P... [more]
A0A4Y0BL205.730e-1144.68Phosphodiesterase OS=Anopheles funestus OX=62324 P... [more]
A0A182X5V45.798e-1143.62GAF domain-containing protein OS=Anopheles quadria... [more]
A0A1Y9IUW39.217e-1143.62Phosphodiesterase OS=Anopheles minimus OX=112268 P... [more]
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BLAST of Phosphodiesterase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphodiesterase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphodiesterase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphodiesterase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO395221.572e-731.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Phosphodiesterase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphodiesterase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000019038.14.650e-53100.00SMESG000019038.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020779 ID=SMED30020779|Name=Phosphodiesterase|organism=Schmidtea mediterranea sexual|type=transcript|length=347bp
GAATTAGCAACGATATAGAATATTCTTCTAGTTATAAAAATGATCCAATT
GCTGAGAAAGTCGCCATATGGCTAAACGAAAATTCAGAATTTACAAAAAA
TTATTTTATTCAAAATGCTACTCGAGGAATGATTGAAAGCTGGAAATCGC
TAAGAAAAGTGTCTTCAAAATCTTCGCAGAGTGATGTCGAATATATAAAT
TATCATTCTTCAAATCAATTAGATCGTAATTCATCAAAATCAGGATCGGG
ATCGAATACTCCAGTCAGAAAAATAAGCAGTCAAGAATTCGATATGGTTG
GATTGCGACCGATTTTGTCCACAAATAGCGATGGATCAATAACTTTT
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protein sequence of SMED30020779-orf-1

>SMED30020779-orf-1 ID=SMED30020779-orf-1|Name=SMED30020779-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
ISNDIEYSSSYKNDPIAEKVAIWLNENSEFTKNYFIQNATRGMIESWKSL
RKVSSKSSQSDVEYINYHSSNQLDRNSSKSGSGSNTPVRKISSQEFDMVG
LRPILSTNSDGSITF
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000099neuron
Vocabulary: molecular function
TermDefinition
GO:00041143',5'-cyclic-nucleotide phosphodiesterase activity
GO:0008081phosphoric diester hydrolase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..92