40S ribosomal protein S21

Overview
Name40S ribosomal protein S21
Smed IDSMED30020776
Length (bp)445
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 40S ribosomal protein S21 (SMED30020776) t-SNE clustered cells

Violin plots show distribution of expression levels for 40S ribosomal protein S21 (SMED30020776) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 40S ribosomal protein S21 (SMED30020776) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 40S ribosomal protein S21 (SMED30020776) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30020776

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30020776SMESG000004954.1 SmedASXL_003600SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
gutSMED30020776SMESG000004954.1 dd_Smed_v4_84_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30020776SMESG000004954.1 dd_Smed_v4_84_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020776SMESG000004954.1 dd_Smed_v4_84_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30020776SMESG000004954.1 dd_Smed_v4_84_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30020776SMESG000004954.1 dd_Smed_v6_84_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 40S ribosomal protein S21 vs. Ensembl Human
Match: RPS21 (ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC:10409])

HSP 1 Score: 103.219 bits (256), Expect = 3.360e-29
Identity = 52/83 (62.65%), Postives = 61/83 (73.49%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y+PRKCSASNRIIGAKDHASI++ +AE D   G   G   +Y I G IR+MGESDDSI RLA  D ++S+NF
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSKNF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Human
Match: RPS21 (ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC:10409])

HSP 1 Score: 98.5969 bits (244), Expect = 2.230e-27
Identity = 50/81 (61.73%), Postives = 59/81 (72.84%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSE 357
            M ND G FVD Y+PRKCSASNRIIGAKDHASI++ +AE D   G   G   +Y I G IR+MGESDDSI RLA  D ++S+
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSK 81          
BLAST of 40S ribosomal protein S21 vs. Ensembl Human
Match: RPS21 (ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC:10409])

HSP 1 Score: 77.7962 bits (190), Expect = 4.199e-19
Identity = 38/62 (61.29%), Postives = 44/62 (70.97%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKM 300
            M ND G FVD Y+PRKCSASNRIIGAKDHASI++ +AE D   G   G   +Y I G IR+M
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRM 62          
BLAST of 40S ribosomal protein S21 vs. Ensembl Human
Match: RPS21 (ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC:10409])

HSP 1 Score: 65.4698 bits (158), Expect = 1.323e-14
Identity = 32/48 (66.67%), Postives = 36/48 (75.00%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTG 258
            M ND G FVD Y+PRKCSASNRIIGAKDHASI++ +AE   E G   G
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVSWEPGRREG 48          
BLAST of 40S ribosomal protein S21 vs. Ensembl Celegans
Match: rps-21 (40S ribosomal protein S21 [Source:UniProtKB/Swiss-Prot;Acc:P49197])

HSP 1 Score: 91.6633 bits (226), Expect = 5.292e-25
Identity = 46/83 (55.42%), Postives = 57/83 (68.67%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTM-TGNKVSYDISGNIRKMGESDDSINRLALKDKMLSEN 360
            M ND G  V+ Y+PRKCS+SNRIIG KDHAS++I   + DPE G M  G    Y I G IR+MGESDD+I RLA KD ++  +
Sbjct:    1 MQNDAGQTVELYVPRKCSSSNRIIGPKDHASVQIDFVDVDPETGRMIPGKSTRYAICGAIRRMGESDDAILRLAQKDGLVPRD 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Fly
Match: RpS21 (gene:FBgn0015521 transcript:FBtr0077621)

HSP 1 Score: 99.3673 bits (246), Expect = 6.205e-28
Identity = 48/83 (57.83%), Postives = 60/83 (72.29%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G  VD Y+PRKCSASNRII AKDHAS+++ + + DPE G  T    +Y I G IR+MGESDD I RLA KD ++++NF
Sbjct:    1 MENDAGENVDLYVPRKCSASNRIIHAKDHASVQLSIVDVDPETGRQTDGSKTYAICGEIRRMGESDDCIVRLAKKDGIITKNF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Fly
Match: RpS21 (gene:FBgn0015521 transcript:FBtr0077623)

HSP 1 Score: 99.3673 bits (246), Expect = 6.205e-28
Identity = 48/83 (57.83%), Postives = 60/83 (72.29%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G  VD Y+PRKCSASNRII AKDHAS+++ + + DPE G  T    +Y I G IR+MGESDD I RLA KD ++++NF
Sbjct:    1 MENDAGENVDLYVPRKCSASNRIIHAKDHASVQLSIVDVDPETGRQTDGSKTYAICGEIRRMGESDDCIVRLAKKDGIITKNF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Fly
Match: RpS21 (gene:FBgn0015521 transcript:FBtr0077622)

HSP 1 Score: 99.3673 bits (246), Expect = 6.205e-28
Identity = 48/83 (57.83%), Postives = 60/83 (72.29%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G  VD Y+PRKCSASNRII AKDHAS+++ + + DPE G  T    +Y I G IR+MGESDD I RLA KD ++++NF
Sbjct:    1 MENDAGENVDLYVPRKCSASNRIIHAKDHASVQLSIVDVDPETGRQTDGSKTYAICGEIRRMGESDDCIVRLAKKDGIITKNF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Fly
Match: RpS21 (gene:FBgn0015521 transcript:FBtr0335131)

HSP 1 Score: 99.3673 bits (246), Expect = 6.205e-28
Identity = 48/83 (57.83%), Postives = 60/83 (72.29%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G  VD Y+PRKCSASNRII AKDHAS+++ + + DPE G  T    +Y I G IR+MGESDD I RLA KD ++++NF
Sbjct:    1 MENDAGENVDLYVPRKCSASNRIIHAKDHASVQLSIVDVDPETGRQTDGSKTYAICGEIRRMGESDDCIVRLAKKDGIITKNF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Fly
Match: RpS21 (gene:FBgn0015521 transcript:FBtr0273293)

HSP 1 Score: 93.9745 bits (232), Expect = 6.703e-26
Identity = 46/81 (56.79%), Postives = 58/81 (71.60%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSE 357
            M ND G  VD Y+PRKCSASNRII AKDHAS+++ + + DPE G  T    +Y I G IR+MGESDD I RLA KD ++++
Sbjct:    1 MENDAGENVDLYVPRKCSASNRIIHAKDHASVQLSIVDVDPETGRQTDGSKTYAICGEIRRMGESDDCIVRLAKKDGIITK 81          
BLAST of 40S ribosomal protein S21 vs. Ensembl Zebrafish
Match: rps21 (ribosomal protein S21 [Source:NCBI gene;Acc:394166])

HSP 1 Score: 97.4413 bits (241), Expect = 4.913e-27
Identity = 48/81 (59.26%), Postives = 59/81 (72.84%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSE 357
            M ND G FVD Y+PRKCSASNRIIGAKDHASI+I +AE D   G   G   +Y I G IR+MGE+DDS+ RLA  D ++++
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQINIAEVDRVTGRFNGQFKTYAICGAIRRMGEADDSLLRLAKTDSIVAK 81          
BLAST of 40S ribosomal protein S21 vs. Ensembl Zebrafish
Match: rps21 (ribosomal protein S21 [Source:NCBI gene;Acc:394166])

HSP 1 Score: 97.4413 bits (241), Expect = 4.913e-27
Identity = 48/81 (59.26%), Postives = 59/81 (72.84%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSE 357
            M ND G FVD Y+PRKCSASNRIIGAKDHASI+I +AE D   G   G   +Y I G IR+MGE+DDS+ RLA  D ++++
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQINIAEVDRVTGRFNGQFKTYAICGAIRRMGEADDSLLRLAKTDSIVAK 81          
BLAST of 40S ribosomal protein S21 vs. Ensembl Xenopus
Match: rps21 (ribosomal protein S21 [Source:NCBI gene;Acc:448708])

HSP 1 Score: 102.064 bits (253), Expect = 9.998e-29
Identity = 52/83 (62.65%), Postives = 60/83 (72.29%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y+PRKCSASNRIIGAKDHASI+I +AE D   G       +Y I G IR+MGESDDSI RLA  D ++S+NF
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQINIAEVDKVTGRFNSQYKTYAICGAIRRMGESDDSILRLAKNDCIVSKNF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Xenopus
Match: rps21 (ribosomal protein S21 [Source:NCBI gene;Acc:448708])

HSP 1 Score: 97.4413 bits (241), Expect = 7.393e-27
Identity = 50/81 (61.73%), Postives = 58/81 (71.60%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSE 357
            M ND G FVD Y+PRKCSASNRIIGAKDHASI+I +AE D   G       +Y I G IR+MGESDDSI RLA  D ++S+
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQINIAEVDKVTGRFNSQYKTYAICGAIRRMGESDDSILRLAKNDCIVSK 81          
BLAST of 40S ribosomal protein S21 vs. Ensembl Mouse
Match: Rps21 (ribosomal protein S21 [Source:MGI Symbol;Acc:MGI:1913731])

HSP 1 Score: 101.679 bits (252), Expect = 1.171e-28
Identity = 51/83 (61.45%), Postives = 60/83 (72.29%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y+PRKCSASNRII AKDHASI++ +AE D   G   G   +Y I G IR+MGESDDSI RLA  D ++S+NF
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIAAKDHASIQMNVAEVDRTTGRFNGQFKTYGICGAIRRMGESDDSILRLAKADGIVSKNF 83          
BLAST of 40S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|Q6RF66|RS21_PECGU (40S ribosomal protein S21 OS=Pectinaria gouldii OX=260746 GN=rps-21 PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 5.583e-35
Identity = 53/85 (62.35%), Postives = 68/85 (80.00%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENFGR 369
            M ND G  VD YIPRKCSA+N I+ AKDHAS++I +AE DP+ G MTG +++Y + G +R+MGE+DDS+NRLA+KDKMLSE FG 
Sbjct:    1 MQNDQGEIVDVYIPRKCSATNNILAAKDHASVQINMAEIDPQTGRMTGKQITYALCGELRRMGEADDSVNRLAMKDKMLSEVFGH 85          
BLAST of 40S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|Q4GXP2|RS21_BIPLU (40S ribosomal protein S21 OS=Biphyllus lunatus OX=197003 GN=RpS21 PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.913e-31
Identity = 53/83 (63.86%), Postives = 63/83 (75.90%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y PRKCSASNRII AKDHASI++C+AE DP+ G MT +  SY + G IR+MGESDD I RL  KD +L++NF
Sbjct:    1 MENDAGEFVDLYCPRKCSASNRIIHAKDHASIQLCVAEVDPQTGRMTDSTKSYALCGAIRRMGESDDCIVRLTKKDGILAKNF 83          
BLAST of 40S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|Q86CT3|RS21_BRABE (40S ribosomal protein S21 OS=Branchiostoma belcheri OX=7741 GN=RPS21 PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.748e-29
Identity = 51/79 (64.56%), Postives = 60/79 (75.95%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKML 351
            M ND G  VD YIPRKCS++NRIIGAKDHASI+I  AE DP  G MTG   +Y I G IR+MGESDD + RLA+KDK++
Sbjct:    1 MQNDAGEVVDLYIPRKCSSTNRIIGAKDHASIQINFAEVDPTTGRMTGQYKTYAICGYIRQMGESDDCLKRLAIKDKII 79          
BLAST of 40S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|Q4GXP3|RS21_AGRLI (40S ribosomal protein S21 OS=Agriotes lineatus OX=292458 GN=RpS21 PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.400e-28
Identity = 52/83 (62.65%), Postives = 60/83 (72.29%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y PRKCSASNRII AKDHASI++ +AE  P  G MT N  SY + G IR+MGESDD I RL  KD +L++NF
Sbjct:    1 MENDAGEFVDLYCPRKCSASNRIIHAKDHASIQLSIAEVVPATGRMTENTKSYALCGAIRRMGESDDCIVRLTKKDGILTKNF 83          
BLAST of 40S ribosomal protein S21 vs. UniProt/SwissProt
Match: sp|P63221|RS21_PIG (40S ribosomal protein S21 OS=Sus scrofa OX=9823 GN=RPS21 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.614e-28
Identity = 52/83 (62.65%), Postives = 61/83 (73.49%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y+PRKCSASNRIIGAKDHASI++ +AE D   G   G   +Y I G IR+MGESDDSI RLA  D ++S+NF
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSKNF 83          
BLAST of 40S ribosomal protein S21 vs. TrEMBL
Match: A0A0U2KE49 (40S ribosomal protein S21 OS=Centropages tenuiremis OX=544689 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 5.886e-30
Identity = 55/83 (66.27%), Postives = 65/83 (78.31%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M N+ G +VD YIPRKCSASNRI+ AKDHASI+I LAE DPE G MTG+  +Y I G IR+MGESDD INRLA KD ++ +NF
Sbjct:    1 MQNEAGEYVDLYIPRKCSASNRILAAKDHASIQINLAEVDPETGRMTGSNKTYAICGAIRRMGESDDCINRLAKKDGLVIKNF 83          
BLAST of 40S ribosomal protein S21 vs. TrEMBL
Match: A0A210Q7S7 (40S ribosomal protein S21 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT08893 PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 2.434e-29
Identity = 54/83 (65.06%), Postives = 64/83 (77.11%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M N+ G FVD YIPRKCSASNRII AKDHASI++ +A+ D   G MTG   +Y I G IR+MGESDDSINRLA KD +L++NF
Sbjct:    1 MQNEGGEFVDMYIPRKCSASNRIIAAKDHASIQLNIADVDETTGRMTGTNTTYAICGTIRRMGESDDSINRLAKKDSILTKNF 83          
BLAST of 40S ribosomal protein S21 vs. TrEMBL
Match: A0A0U2V3E4 (40S ribosomal protein S21 OS=Labidocera rotunda OX=207950 PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.094e-29
Identity = 54/83 (65.06%), Postives = 64/83 (77.11%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M N+ G +VD YIP KCSASNRI+ AKDHASI+I LAE DPE G MTG   +Y I G IR+MGESDD INRLA KD ++++NF
Sbjct:    1 MQNEAGEYVDLYIPMKCSASNRILAAKDHASIQINLAEVDPETGRMTGGYKTYAICGAIRRMGESDDCINRLAKKDGLVAKNF 83          
BLAST of 40S ribosomal protein S21 vs. TrEMBL
Match: A0A4D5R977 (40S ribosomal protein S21 OS=Scolopendra viridis OX=118503 PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.528e-29
Identity = 53/83 (63.86%), Postives = 66/83 (79.52%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M N++G +VD YIPRKCSASNRII AKDHASI+I + E D + G MTGN  +Y I G+IR+MGESDD INRLA KD ++++NF
Sbjct:    1 MQNEVGEYVDLYIPRKCSASNRIIQAKDHASIQINITEVDEQTGRMTGNNKTYAICGSIRRMGESDDCINRLAKKDGIIAKNF 83          
BLAST of 40S ribosomal protein S21 vs. TrEMBL
Match: Q4GXP0 (40S ribosomal protein S21 OS=Sphaerius sp. APV-2005 OX=292460 GN=rpS21e PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 5.226e-29
Identity = 55/83 (66.27%), Postives = 62/83 (74.70%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y PRKCSASNRII AKDHASI+I +AE DP  G MT N  SY + G IR+MGESDD INRL  KD +L++NF
Sbjct:    1 MENDAGEFVDLYCPRKCSASNRIIHAKDHASIQISIAEVDPSTGRMTENSKSYALCGAIRRMGESDDCINRLVKKDGILAKNF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Cavefish
Match: rps21 (ribosomal protein S21 [Source:NCBI gene;Acc:103039508])

HSP 1 Score: 96.2857 bits (238), Expect = 9.529e-27
Identity = 48/81 (59.26%), Postives = 58/81 (71.60%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSE 357
            M ND G FVD Y+PRKCSASNRII AKDHASI+I +AE D   G   G   +Y I G IR+MGE+DDS+ RLA  D ++S+
Sbjct:    1 MQNDAGEFVDMYVPRKCSASNRIIAAKDHASIQINIAEVDKVTGRFNGQFKTYAICGAIRRMGEADDSLLRLAKTDGIVSK 81          
BLAST of 40S ribosomal protein S21 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007607.1 (pep scaffold:Pmarinus_7.0:GL481617:4097:7733:1 gene:ENSPMAG00000006866.1 transcript:ENSPMAT00000007607.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 101.679 bits (252), Expect = 1.747e-29
Identity = 49/83 (59.04%), Postives = 59/83 (71.08%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENF 363
            M ND G FVD Y+PRKCSASNRII AKDHAS+++ +AE DP  G   G   +Y I G IR+MGESDD I RLA  D ++S+ F
Sbjct:    1 MQNDTGEFVDLYVPRKCSASNRIIAAKDHASVQMNIAEVDPSTGRFNGQYKTYAICGPIRRMGESDDCILRLAKDDSIVSKTF 83          
BLAST of 40S ribosomal protein S21 vs. Ensembl Yeast
Match: RPS21A (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication [Source:SGD;Acc:S000001765])

HSP 1 Score: 89.7373 bits (221), Expect = 6.972e-25
Identity = 43/79 (54.43%), Postives = 56/79 (70.89%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKML 351
            M ND G  V+ Y+PRKCSA+NRII A DHAS++I +A+ D E   + G  V+Y +SG +R  GESDDS+NRLA  D +L
Sbjct:    1 MENDKGQLVELYVPRKCSATNRIIKADDHASVQINVAKVDEEGRAIPGEYVTYALSGYVRSRGESDDSLNRLAQNDGLL 79          
BLAST of 40S ribosomal protein S21 vs. Ensembl Yeast
Match: RPS21B (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication [Source:SGD;Acc:S000003672])

HSP 1 Score: 88.9669 bits (219), Expect = 1.227e-24
Identity = 42/79 (53.16%), Postives = 56/79 (70.89%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKML 351
            M ND G  V+ Y+PRKCSA+NRII A DHAS++I +A+ D E   + G  ++Y +SG +R  GESDDS+NRLA  D +L
Sbjct:    1 MENDKGQLVELYVPRKCSATNRIIKADDHASVQINVAKVDEEGRAIPGEYITYALSGYVRSRGESDDSLNRLAQNDGLL 79          
BLAST of 40S ribosomal protein S21 vs. Ensembl Nematostella
Match: EDO39912 (40S ribosomal protein S21 [Source:UniProtKB/TrEMBL;Acc:A7S8Q0])

HSP 1 Score: 83.1889 bits (204), Expect = 5.851e-22
Identity = 40/81 (49.38%), Postives = 57/81 (70.37%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSE 357
            M N+ G  VD YIPRKCS +N II AKDHAS+++ + E D E G  TGN  +Y + G +RK GE+DDS+ ++A +D ++S+
Sbjct:    1 MQNEAGENVDMYIPRKCSVTNSIISAKDHASVQLNVGEID-ETGRFTGNSTTYALCGFLRKQGEADDSLTQIARRDGIISK 80          
BLAST of 40S ribosomal protein S21 vs. Ensembl Medaka
Match: rps21 (ribosomal protein S21 [Source:NCBI gene;Acc:101164286])

HSP 1 Score: 101.293 bits (251), Expect = 1.019e-28
Identity = 51/82 (62.20%), Postives = 60/82 (73.17%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSEN 360
            M ND G FVD Y+PRKCSASNRIIGAKDHASI+I +AE D   G   G   +Y I G IR+MGESDDSI RLA  D ++++N
Sbjct:    1 MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQINIAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKNDTIVAKN 82          
BLAST of 40S ribosomal protein S21 vs. Planmine SMEST
Match: SMESG000004954.1 (SMESG000004954.1)

HSP 1 Score: 182.185 bits (461), Expect = 1.380e-60
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 1
Query:  115 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENFGRKQQ 378
            MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENFGRKQQ
Sbjct:    1 MINDLGVFVDSYIPRKCSASNRIIGAKDHASIRICLAETDPEKGTMTGNKVSYDISGNIRKMGESDDSINRLALKDKMLSENFGRKQQ 88          
The following BLAST results are available for this feature:
BLAST of 40S ribosomal protein S21 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
RPS213.360e-2962.65ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS212.230e-2761.73ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS214.199e-1961.29ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS211.323e-1466.67ribosomal protein S21 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps-215.292e-2555.4240S ribosomal protein S21 [Source:UniProtKB/Swiss... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RpS216.205e-2857.83gene:FBgn0015521 transcript:FBtr0077621[more]
RpS216.205e-2857.83gene:FBgn0015521 transcript:FBtr0077623[more]
RpS216.205e-2857.83gene:FBgn0015521 transcript:FBtr0077622[more]
RpS216.205e-2857.83gene:FBgn0015521 transcript:FBtr0335131[more]
RpS216.703e-2656.79gene:FBgn0015521 transcript:FBtr0273293[more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps214.913e-2759.26ribosomal protein S21 [Source:NCBI gene;Acc:394166... [more]
rps214.913e-2759.26ribosomal protein S21 [Source:NCBI gene;Acc:394166... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps219.998e-2962.65ribosomal protein S21 [Source:NCBI gene;Acc:448708... [more]
rps217.393e-2761.73ribosomal protein S21 [Source:NCBI gene;Acc:448708... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rps211.171e-2861.45ribosomal protein S21 [Source:MGI Symbol;Acc:MGI:1... [more]
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BLAST of 40S ribosomal protein S21 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6RF66|RS21_PECGU5.583e-3562.3540S ribosomal protein S21 OS=Pectinaria gouldii OX... [more]
sp|Q4GXP2|RS21_BIPLU2.913e-3163.8640S ribosomal protein S21 OS=Biphyllus lunatus OX=... [more]
sp|Q86CT3|RS21_BRABE1.748e-2964.5640S ribosomal protein S21 OS=Branchiostoma belcher... [more]
sp|Q4GXP3|RS21_AGRLI1.400e-2862.6540S ribosomal protein S21 OS=Agriotes lineatus OX=... [more]
sp|P63221|RS21_PIG1.614e-2862.6540S ribosomal protein S21 OS=Sus scrofa OX=9823 GN... [more]
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BLAST of 40S ribosomal protein S21 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0U2KE495.886e-3066.2740S ribosomal protein S21 OS=Centropages tenuiremi... [more]
A0A210Q7S72.434e-2965.0640S ribosomal protein S21 OS=Mizuhopecten yessoens... [more]
A0A0U2V3E43.094e-2965.0640S ribosomal protein S21 OS=Labidocera rotunda OX... [more]
A0A4D5R9773.528e-2963.8640S ribosomal protein S21 OS=Scolopendra viridis O... [more]
Q4GXP05.226e-2966.2740S ribosomal protein S21 OS=Sphaerius sp. APV-200... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps219.529e-2759.26ribosomal protein S21 [Source:NCBI gene;Acc:103039... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000007607.11.747e-2959.04pep scaffold:Pmarinus_7.0:GL481617:4097:7733:1 gen... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPS21A6.972e-2554.43Protein component of the small (40S) ribosomal sub... [more]
RPS21B1.227e-2453.16Protein component of the small (40S) ribosomal sub... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO399125.851e-2249.3840S ribosomal protein S21 [Source:UniProtKB/TrEMB... [more]
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BLAST of 40S ribosomal protein S21 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps211.019e-2862.20ribosomal protein S21 [Source:NCBI gene;Acc:101164... [more]
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BLAST of 40S ribosomal protein S21 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000004954.11.380e-60100.00SMESG000004954.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020776 ID=SMED30020776|Name=40S ribosomal protein S21|organism=Schmidtea mediterranea sexual|type=transcript|length=445bp
AATATCAAAAACATATTTGTTTAAATTTCTTAATCTTTAAGTTTTAATAT
TATTTAAAAAGTAAGATATTTATTTTCTTTCATATTTATTGTATTTTACA
TAAATTATTTAAGAATGATTAACGATCTCGGAGTTTTTGTCGATTCATAC
ATCCCAAGAAAATGCAGTGCTTCAAATAGAATTATTGGAGCAAAGGACCA
TGCGTCAATCAGAATTTGCTTAGCTGAAACTGATCCAGAAAAGGGAACAA
TGACTGGAAACAAAGTTTCTTACGATATTTCTGGAAATATTCGCAAAATG
GGAGAGTCCGATGATTCTATCAATAGACTCGCTTTGAAAGACAAAATGTT
AAGTGAAAACTTCGGAAGAAAGCAGCAATAAATTTTATAATAAAATTTTG
GATACTTTAATATACTCTTAATTCAATTTCAAACTATTAACTGTA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005783endoplasmic reticulum
GO:0005791rough endoplasmic reticulum
GO:0005829cytosol
GO:0005622intracellular
GO:0005840ribosome
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR038579Ribosomal_S21e_sf
IPR001931Ribosomal_S21e
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038579Ribosomal protein S21e superfamilyGENE3DG3DSA:3.30.1230.20coord: 1..83
e-value: 4.1E-34
score: 118.4
IPR001931Ribosomal protein S21ePIRSFPIRSF002148RPS21ecoord: 1..87
e-value: 1.1E-33
score: 112.9
IPR001931Ribosomal protein S21ePFAMPF01249Ribosomal_S21ecoord: 1..79
e-value: 2.3E-33
score: 114.2
IPR001931Ribosomal protein S21ePANTHERPTHR1044240S RIBOSOMAL PROTEIN S21coord: 1..80
NoneNo IPR availablePRODOMPD006584coord: 24..85
e-value: 6.0E-18
score: 217.0