GCR103

Overview
NameGCR103
Smed IDSMED30020769
Length (bp)1076
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR103 (SMED30020769) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR103 (SMED30020769) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR103 (SMED30020769) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR103 (SMED30020769) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020769

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30020769h1SMcG0013887 SmedASXL_010872SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30020769h1SMcG0013887 SmedASXL_010872SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30020769h1SMcG0013887 dd_Smed_v4_14122_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020769h1SMcG0013887 dd_Smed_v4_14122_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30020769h1SMcG0013887 dd_Smed_v4_14122_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30020769h1SMcG0013887 dd_Smed_v6_14122_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30020769h1SMcG0013887 OX_Smed_1.0.15391ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GCR103 vs. TrEMBL
Match: A0A193KUC5 (GCR103 OS=Schmidtea mediterranea OX=79327 GN=gcr103 PE=2 SV=1)

HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0
Identity = 322/326 (98.77%), Postives = 323/326 (99.08%), Query Frame = 3 
Query:   90 LDLILGLTSLYLNKLYLITNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSGKFSRGFRCKLSNKYDDLFYFVSLVFKVXXXXXXXXXXXXXXXXXXXXQYDSRDFNNTDSIKFTNSDTPQHSEHSRRISINRXXXXXXXXXXXXXXXXXXPFEIFKVINRNFNINKIKLPKDFEDFLTMRILDKSFNISVYFNQATNWMFYFLLGKSFRTFFCKVIYCRNRTRFQRHYSLKNVRFKPSSEYLLVTSDSRPLKLNSFKLDHRCDTI 1067
            + LILGLTSLYLNKLYLITNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSG FSRGFRCKLSNKYDDLFYFVSLVFKVIIPTLILILSSIIIWRNLSSQYDSRDFNNTDSIKFTNSDTPQHSEHSRRISINRNLSLTLLGMNLTFLLSNLPFEIFKVINRNFNINKIKLPKDFEDFLTMRILDKSFNISVYFNQATNWMFYFLLGKSFRTFFCKVIYCRNRTRFQR YSLKNVRFKPSSEYLLVTSDSRPLKLNSFKLDHRCDTI
Sbjct:   73 ISLILGLTSLYLNKLYLITNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSGNFSRGFRCKLSNKYDDLFYFVSLVFKVIIPTLILILSSIIIWRNLSSQYDSRDFNNTDSIKFTNSDTPQHSEHSRRISINRNLSLTLLGMNLTFLLSNLPFEIFKVINRNFNINKIKLPKDFEDFLTMRILDKSFNISVYFNQATNWMFYFLLGKSFRTFFCKVIYCRNRTRFQRQYSLKNVRFKPSSEYLLVTSDSRPLKLNSFKLDHRCDTI 398

HSP 2 Score: 64.6994 bits (156), Expect = 8.655e-8
Identity = 31/31 (100.00%), Postives = 31/31 (100.00%), Query Frame = 1 
Query:    1 LVPVILLFGCSGTSICIVVLVKTAKRRAKIW 93
            LVPVILLFGCSGTSICIVVLVKTAKRRAKIW
Sbjct:   31 LVPVILLFGCSGTSICIVVLVKTAKRRAKIW 61
[Close]
BLAST of GCR103 vs. TrEMBL
Match: A0A1I8HY28 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.468e-15
Identity = 84/308 (27.27%), Postives = 142/308 (46.10%), Query Frame = 3 
Query:   96 LILGLTSLYLNKLYLITN-----RASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSGKFSRGFRCKL----SNKYDDLFYFVS-LVFKVXXXXXXXXXXXXXXXXXXXXQYDSRDFNNTDSIKFTNSDTPQHSEHSRRISINRXXXXXXXXXXXXXXXXXXPFEIFKV--INRNFNINKIKLPKDFEDFLTMRILDKSFNISVYFNQATNWMFYFLLGKSFRTFFCKVIYCRNRTRFQRHYSLKNVRFKP 983
            L +GL  +YLN +  I        A+  S D RS N   C+ L+   Y+ L  SSW+QVGIS+ R +S   PL  + R   + +++AL + +++ ++ ++  V S++ Y E        G         + K+   +YF S LV +V         +  +   +    Y    F  T       +D  + S+  RRISI       L+GMN TFLL  LP+E++ +    R  ++ +            ++I ++ F + +Y N ATNW+FYF+LG  FR    + ++    T      ++   +F+P
Sbjct:  183 LSVGLPIMYLNIVRAIVKAELSPTATVGSYDPRSWNAPACYGLTILQYYGLIASSWVQVGISLTRCLSAVMPLACRRRLNYAASKRALAVTLAVPIVLAVAAVTSSVKYYERSEANNGTGGGGGSGGVCAQKWKSPYYFYSTLVLRV------FFPAVCLAVSSAGIMYSLHRFKRT-----FPTDQARLSQQQRRISI------ALIGMNSTFLLFALPYELYLIYATERRADLQR----GGPAHAAALKIAERVFAVLMYTNNATNWIFYFVLGHRFRQELQRRLFVCCSTPASSGKAVAQYKFRP 469

HSP 2 Score: 32.7278 bits (73), Expect = 1.468e-15
Identity = 12/33 (36.36%), Postives = 24/33 (72.73%), Query Frame = 1 
Query:    1 LVPVILLFGCSGTSICIVVLVK--TAKRRAKIW 93
            LVP++ + GC+GT +C+ VL++  + ++ A+ W
Sbjct:  139 LVPLLYVSGCAGTIVCVTVLLRRGSTQKEARPW 171
[Close]
BLAST of GCR103 vs. Planmine SMEST
Match: SMESG000075889.1 (SMESG000075889.1)

HSP 1 Score: 260.381 bits (664), Expect = 1.346e-86
Identity = 135/144 (93.75%), Postives = 136/144 (94.44%), Query Frame = 3 
Query:   75 KASKNLDLILGLTSLYLNKLYLITNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSGKFSRGFRCKLSNKYDDLFYFVSLVFKV 506
            K +K    I  LTSLYLNKLYLITNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSG FSRGFRCKLSNKYDDLFYFVSLVFKV
Sbjct:   52 KTAKRRAKIWSLTSLYLNKLYLITNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSGNFSRGFRCKLSNKYDDLFYFVSLVFKV 195

HSP 2 Score: 64.3142 bits (155), Expect = 8.238e-12
Identity = 31/31 (100.00%), Postives = 31/31 (100.00%), Query Frame = 1 
Query:    1 LVPVILLFGCSGTSICIVVLVKTAKRRAKIW 93
            LVPVILLFGCSGTSICIVVLVKTAKRRAKIW
Sbjct:   31 LVPVILLFGCSGTSICIVVLVKTAKRRAKIW 61
[Close]
BLAST of GCR103 vs. Planmine SMEST
Match: SMESG000078225.1 (SMESG000078225.1)

HSP 1 Score: 164.851 bits (416), Expect = 3.761e-47
Identity = 128/301 (42.52%), Postives = 189/301 (62.79%), Query Frame = 3 
Query:   93 DLILGLTSLYLNKLYLITNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNSTLIYDEVYSGKFSRGFRCKLSNKYDDLFYFVSLVFKVXXXXXXXXXXXXXXXXXXXXQYDSRD----FNNTDSIKFTNSDTPQ----HSEHSRRISINRXXXXXXXXXXXXXXXXXXPFEIFKVINRNFNINKIKLPKDFEDFLTMRILDKSFNISVYFNQATNWMFYFLLGKSFRTFFCKVIYCRNRTRFQRHYSLKNV 971
             LI GL++LYL+KL LI ++    + D +SS  FVC+F S  ++F+L LSSW+Q GIS +R +S S PL ++T+ T S AR  L++ I   L+ S+++VN T+ Y E Y    ++G RC+L  K+ + F+  SL+ +VIIP L+L+LSS +IW++L+ Q   R       N D     NS +P+    HS HS     +RN+S  LL MNL FLL+NLPFEIFK+I +N+++  IK   +   ++  RI DK FNI VY NQ+TNW+FYF+LGK+FR     +  C+     +  ++ KN+
Sbjct:   66 GLIFGLSTLYLSKLNLILSKPM-WNFDIKSSQEFVCYFNSNCIFFSLLLSSWLQAGISALRAVSTSQPLKLRTKITTSSARIYLILLIGTALILSLLIVNITVEYKEEYLRNRTQGHRCRLKKKFINTFFVFSLIVRVIIPILLLLLSSFMIWKSLNRQQHERRQSCLLGNHDLSASRNSPSPRGKRIHSPHSN----HRNISCMLLWMNLAFLLANLPFEIFKIIYKNYDVLSIKTKNEMYLYILSRIFDKFFNILVYLNQSTNWLFYFMLGKNFRKDLVILFRCKKHNWSRGKWNSKNI 361
[Close]
BLAST of GCR103 vs. Planmine SMEST
Match: SMESG000064805.1 (SMESG000064805.1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.763e-14
Identity = 89/277 (32.13%), Postives = 141/277 (50.90%), Query Frame = 3 
Query:   96 LILGLTSLYLNKLYLI----TNRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTR-FTASVARKALVIHISITLLFSIILVNSTLIYDEVYSGKFSRGFRCKLSNKYDDLFYFVSLVFKVXXXXXXXXXXXXXXXXXXXXQYDSRDFNNTDSIKFTNSDTPQHSEHSRRISINRXXXXXXXXXXXXXXXXXXPFEIFKVIN----RNFNINKIKLPKDFEDFLTMRILDKSFNISVYFNQATNWMFYFLLGKSFR 899
            L +GL+ LY++++  I     N  +   +  ++SN + C  ++  +Y  L LS W+QVGIS+ R +++  P   +        +  AL+  I + L  + +L+  +L Y         +  + K S+K+   FYF SL  KVI+P ++L+ SS +I + L    D+    N D   +  S   +  +  R IS+       LLGMN T LL  LPFE++K+I+     +    K  L       +T R+ DK  NI +Y N ATNW+FYF+LGK FR
Sbjct:   89 LCVGLSILYISRISFIFPFSQNSTNIFQDGIKNSNKYSCFIINYMVYVGLLLSVWVQVGISMSRAVALKWPNIPRNNSIRLRNSYYALIFCIFMCLSIAFVLIFDSLEYRGTDRCPIFKICKVK-SSKFRKFFYF-SLTVKVIVPIILLLSSSYVITQKLKQSVDNYR-RNFDRYNYYFSRLKKMQQ--RHISLT------LLGMNSTLLLFVLPFEVYKIIHFYHYEDIKETKPDLKAGQSPHVTARLFDKLLNIILYTNNATNWLFYFMLGKRFR 354

HSP 2 Score: 26.9498 bits (58), Expect = 2.763e-14
Identity = 12/33 (36.36%), Postives = 20/33 (60.61%), Query Frame = 1 
Query:    4 VPVILLFGCSGTSICIVVLVK--TAKRRAKIWI 96
             P +L+FG SGT IC++V+ K  + +   + W 
Sbjct:   46 TPCLLIFGVSGTVICLIVINKHRSGQHELRAWF 78
[Close]
BLAST of GCR103 vs. Planmine SMEST
Match: SMESG000061776.1 (SMESG000061776.1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.758e-9
Identity = 41/148 (27.70%), Postives = 74/148 (50.00%), Query Frame = 3 
Query:   93 DLILGLTSLYLNKLYLIT----NRASSISEDFRSSNIFVCHFLSTGLYFTLFLSSWMQVGISVIRTISISCPLFIKTRFTASVARK------ALVIHISITLLFSIILVNSTLIYDEVYSGKFSRGFRCKLSNKYDDLFYFVSLVFKV 506
             L++GL+  Y ++++ I         +I  D R  + F C+FL+  +YF L +S+W+QVGISV+R +S      +K++     +RK      AL + ++  ++ + IL+   + Y         +   C+LS      F++ SL  KV
Sbjct:   97 SLLVGLSIFYSSRIHFIFPTLFREDEAIQFDMRQRSAFNCYFLNGLVYFGLLVSTWIQVGISVLRVLS------VKSKSPNPHSRKTKKYAIALGVCVTSCVIITCILIGVNIEYTFPKPCPLHK--VCRLSIVKSRYFFYSSLTIKV 236

HSP 2 Score: 28.8758 bits (63), Expect = 1.758e-9
Identity = 12/34 (35.29%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    1 LVPVILLFGCSGTSICIVVLVK--TAKRRAKIWI 96
             VP++++FG  GT +C++VL+K  T     ++W 
Sbjct:   54 FVPLLIMFGEIGTLLCLIVLLKRRTGNAETRMWF 87
The following BLAST results are available for this feature:
BLAST of GCR103 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A193KUC58.655e-898.77GCR103 OS=Schmidtea mediterranea OX=79327 GN=gcr10... [more]
A0A1I8HY281.468e-1527.27Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of GCR103 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR103 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000075889.11.346e-8693.75SMESG000075889.1[more]
SMESG000078225.13.761e-4742.52SMESG000078225.1[more]
SMESG000064805.12.763e-1432.13SMESG000064805.1[more]
SMESG000061776.11.758e-927.70SMESG000061776.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020769 ID=SMED30020769|Name=GCR103|organism=Schmidtea mediterranea sexual|type=transcript|length=1076bp
CTAGTTCCCGTTATTTTACTTTTCGGATGCAGCGGAACGTCAATTTGCAT
TGTTGTTTTGGTTAAAACGGCAAAAAGGCGAGCAAAAATTTGGATTTGAT
ATTAGGTCTGACCTCGCTTTATTTAAACAAATTGTATTTAATTACAAATC
GAGCCAGTTCAATATCAGAAGACTTTCGTTCATCGAATATTTTCGTTTGC
CATTTCCTCTCAACTGGTCTTTATTTCACGCTATTTTTATCAAGCTGGAT
GCAAGTTGGAATATCTGTCATTCGTACTATTTCTATTTCCTGTCCACTCT
TCATTAAAACAAGATTCACGGCAAGTGTGGCAAGAAAAGCCCTCGTGATT
CACATAAGTATTACACTATTATTTTCAATTATTCTTGTCAATTCCACTTT
GATCTACGATGAAGTTTACTCAGGAAAATTTTCCCGAGGATTTCGTTGTA
AATTGAGCAATAAATACGATGATTTGTTTTACTTTGTCAGTTTGGTGTTT
AAAGTGATCATTCCTACTCTGATTCTGATTTTGTCCTCGATAATTATATG
GAGAAATTTATCAAGCCAGTACGACAGTCGAGATTTTAATAATACAGATT
CAATTAAATTTACTAATTCCGACACTCCGCAACATTCTGAACATTCTCGG
CGGATATCAATTAATAGAAACCTCTCTTTGACACTCTTGGGAATGAATTT
AACCTTTTTATTATCAAACCTCCCCTTCGAAATATTCAAAGTGATCAACA
GAAATTTCAATATTAACAAAATCAAATTACCGAAAGATTTTGAAGATTTT
TTAACGATGAGGATTTTGGATAAATCTTTTAACATTTCCGTTTATTTCAA
CCAGGCCACTAATTGGATGTTTTACTTTCTGTTGGGGAAATCTTTTCGGA
CGTTTTTTTGCAAAGTTATCTACTGTCGAAATAGAACAAGATTCCAACGG
CATTATTCGTTGAAAAATGTTCGGTTTAAGCCGTCGAGTGAATATTTATT
AGTAACAAGCGATAGCAGACCGTTGAAACTCAATTCCTTCAAATTGGACC
ATCGTTGTGACACGATCTAGTGCGGA
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protein sequence of SMED30020769-orf-1

>SMED30020769-orf-1 ID=SMED30020769-orf-1|Name=SMED30020769-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=274bp
MQVGISVIRTISISCPLFIKTRFTASVARKALVIHISITLLFSIILVNST
LIYDEVYSGKFSRGFRCKLSNKYDDLFYFVSLVFKVIIPTLILILSSIII
WRNLSSQYDSRDFNNTDSIKFTNSDTPQHSEHSRRISINRNLSLTLLGMN
LTFLLSNLPFEIFKVINRNFNINKIKLPKDFEDFLTMRILDKSFNISVYF
NQATNWMFYFLLGKSFRTFFCKVIYCRNRTRFQRHYSLKNVRFKPSSEYL
LVTSDSRPLKLNSFKLDHRCDTI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 1..88
e-value: 1.6E-6
score: 29.2