SMED30020735

Overview


Name	SMED30020735
Smed ID	SMED30020735
Length (bp)	596

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30020735 (SMED30020735) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020735 (SMED30020735) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020735 (SMED30020735) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020735 (SMED30020735) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30020735

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30020735	SMESG000062458.1	Contig4602	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30020735	SMESG000003862.1	Contig4602	GPL15192	PMID:23079596 Forsthoefel et al., 2012
parapharyngeal region	SMED30020735	SMESG000003887.1	dd_Smed_v4_10285_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30020735	SMESG000003887.1	dd_Smed_v4_10285_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30020735	SMESG000003862.1	Contig14391	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30020735	SMESG000003862.1	Contig14391	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30020735	SMESG000003862.1	Contig6733	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30020735	SMESG000003862.1	Contig6733	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30020735	SMESG000003862.1	dd_Smed_v6_3667_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30020735 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.022789:4751..5382 -	2724
v31.002755:82845..83475 +	2590

Homology

[Close]

BLAST of SMED30020735 vs. Ensembl Human
Match: PLK1 (polo like kinase 1 [Source:HGNC Symbol;Acc:HGNC:9077])

HSP 1 Score: 51.9878 bits (123), Expect = 1.754e-7
Identity = 30/105 (28.57%), Postives = 52/105 (49.52%), Query Frame = -2

Query:  269 DYLAPEILDNKPWNIKADVWSFGIILLEIITGK-CFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQF 580
            +Y+APE+L  K  + + DVWS G I+  ++ GK  F  + +K        + +I  ++Y  P+ IN     +I   L  DP  R ++  LL+ +   S   P++ 
Sbjct:  216 NYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKET------YLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARL 314

[Close]

BLAST of SMED30020735 vs. Ensembl Human
Match: PAK4 (p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;Acc:HGNC:16059])

HSP 1 Score: 49.6766 bits (117), Expect = 2.575e-7
Identity = 31/113 (27.43%), Postives = 53/113 (46.90%), Query Frame = -2

Query:  254 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFN-----AIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMN 577
            ++APE++   P+  + D+WS GI+++E++ G+   FN     A+K    N     K  +        ++ S KG +D  LV DP  R +   LL    L     P+  + +M 
Sbjct:   39 WMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHK-------VSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMR 144

[Close]

BLAST of SMED30020735 vs. Ensembl Human
Match: PAK4 (p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;Acc:HGNC:16059])

HSP 1 Score: 49.2914 bits (116), Expect = 1.342e-6
Identity = 31/113 (27.43%), Postives = 53/113 (46.90%), Query Frame = -2

Query:  254 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFN-----AIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMN 577
            ++APE++   P+  + D+WS GI+++E++ G+   FN     A+K    N     K  +        ++ S KG +D  LV DP  R +   LL    L     P+  + +M 
Sbjct:  328 WMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHK-------VSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMR 433

[Close]

Query:  254 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFN-----AIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMN 577
            ++APE++   P+  + D+WS GI+++E++ G+   FN     A+K    N     K  +        ++ S KG +D  LV DP  R +   LL    L     P+  + +M 
Sbjct:  328 WMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHK-------VSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMR 433

[Close]

BLAST of SMED30020735 vs. Ensembl Human
Match: PAK4 (p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;Acc:HGNC:16059])

HSP 1 Score: 49.2914 bits (116), Expect = 1.553e-6
Identity = 31/113 (27.43%), Postives = 53/113 (46.90%), Query Frame = -2

Query:  254 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFN-----AIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMN 577
            ++APE++   P+  + D+WS GI+++E++ G+   FN     A+K    N     K  +        ++ S KG +D  LV DP  R +   LL    L     P+  + +M 
Sbjct:  391 WMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHK-------VSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMR 496

[Close]

BLAST of SMED30020735 vs. Ensembl Celegans
Match: T21G5.1 (Tyrosine-protein kinase [Source:UniProtKB/TrEMBL;Acc:O02064])

HSP 1 Score: 50.447 bits (119), Expect = 2.338e-7
Identity = 27/85 (31.76%), Postives = 49/85 (57.65%), Query Frame = -2

Query:  323 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISV 577
            +LAPEI+ +K +++K+D+W+FGI++ EI       +  + N  +  A    +DY +  FP+        VID+C  +DP+ R ++
Sbjct:  356 WLAPEIMQDKVYSLKSDIWAFGIMIWEIFAEGDEPYPGL-NNTQARAKIVVLDY-RMKFPDEAPTELVQVIDTCWKKDPDMRQTM 438

[Close]

BLAST of SMED30020735 vs. Ensembl Celegans
Match: ZK524.4 (pep chromosome:WBcel235:I:7460858:7466424:-1 gene:WBGene00013994.1 transcript:ZK524.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK524.4)

HSP 1 Score: 48.9062 bits (115), Expect = 9.354e-7
Identity = 30/92 (32.61%), Postives = 51/92 (55.43%), Query Frame = -2

Query:  308 YLAPEILDNKPWNIKA-DVWSFGIILLEIITGKCFNFNAIKNENKNDAD-FFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLS 577
            Y APEIL    ++  A DVWS G+IL  ++ G+      +  +  ND++   KI   KY  P+ +++  + +I S LV +P  R S++ ++S
Sbjct:  190 YSAPEILLGDSYDAPAVDVWSLGVILYMLVCGR------LPFQEANDSETLTKILDCKYSIPDVLSDECRNLIQSMLVREPQKRASLEKIVS 275

[Close]

BLAST of SMED30020735 vs. Ensembl Celegans
Match: air-1 (Aurora/Ipl1 Related kinase; Aurora/Ipl1-related protein kinase 1 [Source:UniProtKB/TrEMBL;Acc:G5EDL3])

HSP 1 Score: 48.1358 bits (113), Expect = 1.070e-6
Identity = 25/98 (25.51%), Postives = 50/98 (51.02%), Query Frame = -2

Query:  287 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP 580
            DYLAPE++ N+P +   D+W+ GI+L E++ G    +    N+   D    +I   K   P  + +    +I++ + ++P  R+ +     +D++  P
Sbjct:  207 DYLAPEMVSNQPHDFNVDIWAIGILLFEMLVG----YAPFANQT-GDKLIARIKECKIYIPSVVTDGAASLINAIIKKEPQERLPL-----VDIMAHP 294

[Close]

Query:  287 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP 580
            DYLAPE++ N+P +   D+W+ GI+L E++ G    +    N+   D    +I   K   P  + +    +I++ + ++P  R+ +     +D++  P
Sbjct:  207 DYLAPEMVSNQPHDFNVDIWAIGILLFEMLVG----YAPFANQT-GDKLIARIKECKIYIPSVVTDGAASLINAIIKKEPQERLPL-----VDIMAHP 294

[Close]

BLAST of SMED30020735 vs. Ensembl Celegans
Match: pkc-3 (Protein kinase C-like 3 [Source:UniProtKB/Swiss-Prot;Acc:Q19266])

HSP 1 Score: 48.1358 bits (113), Expect = 1.274e-6
Identity = 23/91 (25.27%), Postives = 49/91 (53.85%), Query Frame = -2

Query:  317 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKC-FNFNAIKNENKNDADF-FKIDYSKYV-FPEGINESFKGVIDSCLVEDPNGRISVQM 580
            +Y+APEIL    +    D W+ G+++ E++ G+  F+   ++N  +N  D+ F+I   + +  P  ++    G++   L +DP  R+  ++
Sbjct:  417 NYIAPEILRGDEYGFSVDWWALGVLMFEMMAGRSPFDIVGMQNSEENTEDYLFQIILERQIRIPRSLSVRASGILKGFLNKDPTERLGCKL 507

[Close]

BLAST of SMED30020735 vs. Ensembl Fly
Match: aurA (gene:FBgn0000147 transcript:FBtr0082483)

HSP 1 Score: 53.5286 bits (127), Expect = 2.599e-8
Identity = 26/90 (28.89%), Postives = 47/90 (52.22%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE++  KP     D+WS G++  E++ G    ++  KN ++      K+DY     PE I+++   +I   LV +P  R+ +  ++
Sbjct:  317 DYLPPEMVQGKPHTKNVDLWSLGVLCFELLVGHAPFYS--KNYDETYKKILKVDYK---LPEHISKAASHLISKLLVLNPQHRLPLDQVM 401

[Close]

BLAST of SMED30020735 vs. Ensembl Fly
Match: Ret (gene:FBgn0011829 transcript:FBtr0081493)

HSP 1 Score: 51.9878 bits (123), Expect = 9.355e-8
Identity = 29/90 (32.22%), Postives = 48/90 (53.33%), Query Frame = -2

Query:  308 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLS 577
            ++APE L +  +  K+DVWSFG++  E+IT     +  I  +N       K  Y +   PE  +E+   ++ +C  ++PNGR S + L S
Sbjct:  954 WMAPESLADHVYTSKSDVWSFGVLCWELITLGASPYPGIAPQNL--WSLLKTGY-RMDRPENCSEAVYSIVRTCWADEPNGRPSFKFLAS 1040

[Close]

BLAST of SMED30020735 vs. Ensembl Fly
Match: Ret (gene:FBgn0011829 transcript:FBtr0081494)

HSP 1 Score: 51.9878 bits (123), Expect = 9.355e-8
Identity = 29/90 (32.22%), Postives = 48/90 (53.33%), Query Frame = -2

Query:  308 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLS 577
            ++APE L +  +  K+DVWSFG++  E+IT     +  I  +N       K  Y +   PE  +E+   ++ +C  ++PNGR S + L S
Sbjct:  954 WMAPESLADHVYTSKSDVWSFGVLCWELITLGASPYPGIAPQNL--WSLLKTGY-RMDRPENCSEAVYSIVRTCWADEPNGRPSFKFLAS 1040

[Close]

BLAST of SMED30020735 vs. Ensembl Fly
Match: Ret (gene:FBgn0011829 transcript:FBtr0081495)

HSP 1 Score: 51.9878 bits (123), Expect = 9.355e-8
Identity = 29/90 (32.22%), Postives = 48/90 (53.33%), Query Frame = -2

Query:  308 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLS 577
            ++APE L +  +  K+DVWSFG++  E+IT     +  I  +N       K  Y +   PE  +E+   ++ +C  ++PNGR S + L S
Sbjct:  954 WMAPESLADHVYTSKSDVWSFGVLCWELITLGASPYPGIAPQNL--WSLLKTGY-RMDRPENCSEAVYSIVRTCWADEPNGRPSFKFLAS 1040

[Close]

BLAST of SMED30020735 vs. Ensembl Fly
Match: inaC (gene:FBgn0004784 transcript:FBtr0087047)

HSP 1 Score: 51.9878 bits (123), Expect = 9.721e-8
Identity = 29/85 (34.12%), Postives = 46/85 (54.12%), Query Frame = -2

Query:  329 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFK-IDYSKYVFPEGINESFKGVIDSCLVEDPNGRI 580
            +Y+APEI+   P++I AD WSFG++L E + G+         E  ++   F+ I   K VFP+  +     +I S L + PN R+
Sbjct:  535 NYMAPEIVSYDPYSIAADWWSFGVLLFEFMAGQA------PFEGDDETTVFRNIKDKKAVFPKHFSVEAMDIITSFLTKKPNNRL 613

[Close]

BLAST of SMED30020735 vs. Ensembl Zebrafish
Match: stk17al (serine/threonine kinase 17a like [Source:ZFIN;Acc:ZDB-GENE-061103-427])

HSP 1 Score: 55.0694 bits (131), Expect = 8.128e-9
Identity = 30/90 (33.33%), Postives = 51/90 (56.67%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            +Y+APEILD +P +   D+WS G++   ++TG+       K E   +     +DYS+ VF +GI++     I S L+++P  R +V+  L
Sbjct:  192 EYVAPEILDYEPISTATDMWSIGVLTYVMLTGESPFLGEEKQETFLNISQVNVDYSQDVF-QGISDLAVNFIQSLLIKNPRKRSTVEQCL 280

[Close]

BLAST of SMED30020735 vs. Ensembl Zebrafish
Match: pak4 (p21 protein (Cdc42/Rac)-activated kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040704-69])

HSP 1 Score: 50.0618 bits (118), Expect = 6.279e-7
Identity = 33/112 (29.46%), Postives = 54/112 (48.21%), Query Frame = -2

Query:  257 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFN-----AIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMM 577
            ++APE++   P+  + D+WSFGI+++E++ G+   FN     A+K    N     K  +        ++   KG +D  LV DP  R + Q LL    L     PS  + +M
Sbjct:  554 WMAPELISRLPYGPEVDIWSFGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPKLKNLHK-------VSPLLKGFLDRMLVRDPAQRATAQELLKHPFLTKAGPPSCIVPLM 658

[Close]

BLAST of SMED30020735 vs. Ensembl Zebrafish
Match: plk1 (polo-like kinase 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-021115-7])

HSP 1 Score: 49.2914 bits (116), Expect = 1.015e-6
Identity = 31/98 (31.63%), Postives = 48/98 (48.98%), Query Frame = -2

Query:  290 DYLAPEILDNKPWNIKADVWSFGIILLEIITGK-CFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 580
            +Y+APE+L  K  + + D+WS G IL  ++ GK  F  + +K        + +I  ++Y  P  IN     +I   L  DP  R SV  LL+ +   S
Sbjct:  202 NYIAPEVLCKKGHSFEVDIWSLGCILYTLLVGKPPFETSCLKET------YIRIKKNEYSVPRHINPVASALIRRMLHADPTLRPSVAELLTDEFFTS 293

[Close]

Query:  290 DYLAPEILDNKPWNIKADVWSFGIILLEIITGK-CFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 580
            +Y+APE+L  K  + + D+WS G IL  ++ GK  F  + +K        + +I  ++Y  P  IN     +I   L  DP  R SV  LL+ +   S
Sbjct:  202 NYIAPEVLCKKGHSFEVDIWSLGCILYTLLVGKPPFETSCLKET------YIRIKKNEYSVPRHINPVASALIRRMLHADPTLRPSVAELLTDEFFTS 293

[Close]

BLAST of SMED30020735 vs. Ensembl Zebrafish
Match: stk40 (serine/threonine kinase 40 [Source:ZFIN;Acc:ZDB-GENE-030131-4831])

HSP 1 Score: 48.521 bits (114), Expect = 1.687e-6
Identity = 29/100 (29.00%), Postives = 58/100 (58.00%), Query Frame = -2

Query:  290 YLAPEILDNKPWNIK-ADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPE--GINESFKGVIDSCLVEDPNGRIS-VQMLLSIDLLIS 577
            Y++P++L  +P+  K +D+W+ G++L  ++ G+   +++I  E      F KI  ++Y  PE   ++ES   +I   LV DP  R++  ++L S+  +I+
Sbjct:  240 YISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQEL-----FRKIKGAEYSIPEDGRVSESTVCLIRKLLVLDPQQRLTATEVLESLSAIIA 334

[Close]

BLAST of SMED30020735 vs. Ensembl Xenopus
Match: ENSXETT00000027062.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:2:26945136:26947326:1 gene:ENSXETG00000013169.1 transcript:ENSXETT00000027062.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.0618 bits (118), Expect = 2.775e-7
Identity = 23/84 (27.38%), Postives = 40/84 (47.62%), Query Frame = -2

Query:  329 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRI 580
             Y+APE+L   P+N  AD WS G++L  + TGK      +  E  + +   ++  + Y  PE  +     ++   L + P  R+
Sbjct:   37 QYMAPEVLSGGPYNHSADWWSLGVLLFALATGKF----PVAPERDHVSMLERVSQASYDMPEHFSHGLSHLLKELLCKVPRLRL 116

[Close]

BLAST of SMED30020735 vs. Ensembl Xenopus
Match: stk40 (serine/threonine kinase 40 [Source:NCBI gene;Acc:100145549])

HSP 1 Score: 48.9062 bits (115), Expect = 1.529e-6
Identity = 27/92 (29.35%), Postives = 52/92 (56.52%), Query Frame = -2

Query:  311 YLAPEILDNKPWNIK-ADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPE--GINESFKGVIDSCLVEDPNGRISVQMLL 577
            Y++P++L  +P+  K +D+W+ G++L  ++ G+   +++I  E      F KI  ++Y  PE   ++ES   +I   LV DP  R++   +L
Sbjct:  239 YISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQEL-----FRKIKAAEYSIPEDGRVSESTVCLIRKLLVLDPQQRLTASEVL 325

[Close]

BLAST of SMED30020735 vs. Ensembl Xenopus
Match: sap130 (Sin3A associated protein 130kDa [Source:Xenbase;Acc:XB-GENE-6083596])

HSP 1 Score: 48.9062 bits (115), Expect = 1.598e-6
Identity = 28/95 (29.47%), Postives = 48/95 (50.53%), Query Frame = -2

Query:  311 YLAPEILDN-KPWNIKADVWSFGIILLEIITG-----KCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 577
            ++APE+++  + ++ KAD+WSFGI  +E+ TG     K      +    +ND    +         +   +SF+ +I SCL +DP  R +   LL
Sbjct:  193 WMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVQDKEMLKKYGKSFRKMISSCLQKDPEKRPTAAELL 287

[Close]

BLAST of SMED30020735 vs. Ensembl Xenopus
Match: stk40 (serine/threonine kinase 40 [Source:NCBI gene;Acc:100145549])

HSP 1 Score: 48.9062 bits (115), Expect = 1.720e-6
Identity = 27/92 (29.35%), Postives = 52/92 (56.52%), Query Frame = -2

Query:  311 YLAPEILDNKPWNIK-ADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPE--GINESFKGVIDSCLVEDPNGRISVQMLL 577
            Y++P++L  +P+  K +D+W+ G++L  ++ G+   +++I  E      F KI  ++Y  PE   ++ES   +I   LV DP  R++   +L
Sbjct:  246 YISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQEL-----FRKIKAAEYSIPEDGRVSESTVCLIRKLLVLDPQQRLTASEVL 332

[Close]

BLAST of SMED30020735 vs. Ensembl Xenopus
Match: ENSXETT00000029423.1 (serine/threonine-protein kinase 17A-like [Source:NCBI gene;Acc:101732252])

HSP 1 Score: 48.521 bits (114), Expect = 1.831e-6
Identity = 29/90 (32.22%), Postives = 45/90 (50.00%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            +Y+APE+L  +P +   D+WS G++   ++TG        K E   +     I YS+  F +GI+E     I S LV++P  RI     L
Sbjct:  191 EYVAPEVLSYEPISTATDMWSVGVLTYVMLTGVSPFLGDTKQETFLNISQVNIQYSQEDF-QGISEPAIDFIKSLLVKNPRKRIRADQCL 279

[Close]

BLAST of SMED30020735 vs. Ensembl Mouse
Match: Plk1 (polo like kinase 1 [Source:MGI Symbol;Acc:MGI:97621])

HSP 1 Score: 52.373 bits (124), Expect = 1.036e-7
Identity = 30/105 (28.57%), Postives = 52/105 (49.52%), Query Frame = -2

Query:  269 DYLAPEILDNKPWNIKADVWSFGIILLEIITGK-CFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQF 580
            +Y+APE+L  K  + + DVWS G I+  ++ GK  F  + +K        + +I  ++Y  P+ IN     +I   L  DP  R ++  LL+ +   S   P++ 
Sbjct:  216 NYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKET------YLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTIHELLNDEFFTSGYIPARL 314

[Close]

BLAST of SMED30020735 vs. Ensembl Mouse
Match: Aurkc (aurora kinase C [Source:MGI Symbol;Acc:MGI:1321119])

HSP 1 Score: 48.1358 bits (113), Expect = 1.108e-6
Identity = 24/90 (26.67%), Postives = 45/90 (50.00%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE++  KP+N   D+W  G++  E++ GK    ++  +E      + +I    + FP  +    + +I   L   P+ R+S+  +L
Sbjct:   85 DYLPPEMIAQKPYNEMVDLWCIGVLCYELLVGKPPFESSTSSET-----YRRIRQVDFKFPSSVPAGAQDLISKLLRYHPSERLSLAQVL 169

[Close]

BLAST of SMED30020735 vs. Ensembl Mouse
Match: Aurkc (aurora kinase C [Source:MGI Symbol;Acc:MGI:1321119])

HSP 1 Score: 48.1358 bits (113), Expect = 1.824e-6
Identity = 24/90 (26.67%), Postives = 45/90 (50.00%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE++  KP+N   D+W  G++  E++ GK    ++  +E      + +I    + FP  +    + +I   L   P+ R+S+  +L
Sbjct:  177 DYLPPEMIAQKPYNEMVDLWCIGVLCYELLVGKPPFESSTSSET-----YRRIRQVDFKFPSSVPAGAQDLISKLLRYHPSERLSLAQVL 261

[Close]

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE++  KP+N   D+W  G++  E++ GK    ++  +E      + +I    + FP  +    + +I   L   P+ R+S+  +L
Sbjct:  177 DYLPPEMIAQKPYNEMVDLWCIGVLCYELLVGKPPFESSTSSET-----YRRIRQVDFKFPSSVPAGAQDLISKLLRYHPSERLSLAQVL 261

[Close]

BLAST of SMED30020735 vs. Ensembl Mouse
Match: Aurkc (aurora kinase C [Source:MGI Symbol;Acc:MGI:1321119])

HSP 1 Score: 48.1358 bits (113), Expect = 2.153e-6
Identity = 24/90 (26.67%), Postives = 45/90 (50.00%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE++  KP+N   D+W  G++  E++ GK    ++  +E      + +I    + FP  +    + +I   L   P+ R+S+  +L
Sbjct:  216 DYLPPEMIAQKPYNEMVDLWCIGVLCYELLVGKPPFESSTSSET-----YRRIRQVDFKFPSSVPAGAQDLISKLLRYHPSERLSLAQVL 300

[Close]

BLAST of SMED30020735 vs. UniProt/SwissProt
Match: sp|Q08942|NRKA_TRYBB (Putative serine/threonine-protein kinase A OS=Trypanosoma brucei brucei OX=5702 GN=NRKA PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.745e-9
Identity = 37/93 (39.78%), Postives = 52/93 (55.91%), Query Frame = -2

Query:  305 YLAPEILDNKPWNIKADVWSFGIILLEII-TGKCFNFNAIKNENKNDADFFKIDYSKYV-FPEGINESFKGVIDSCLVEDPNGRISVQMLLSI 577
            YLAPE+ +NK +N KADVWS G++L EI+   K F+ + +K          K+    Y   P+  +  FK V+D  LV DPN R SV+ +  I
Sbjct:  190 YLAPELWNNKRYNKKADVWSLGVLLYEIMGMKKPFSASNLKGL------MSKVLAGTYAPLPDSFSSEFKRVVDGILVADPNDRPSVREIFQI 276

[Close]

BLAST of SMED30020735 vs. UniProt/SwissProt
Match: sp|Q03428|NRKB_TRYBB (Putative serine/threonine-protein kinase B OS=Trypanosoma brucei brucei OX=5702 GN=NRKB PE=2 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 3.737e-8
Identity = 35/88 (39.77%), Postives = 49/88 (55.68%), Query Frame = -2

Query:  320 YLAPEILDNKPWNIKADVWSFGIILLEII-TGKCFNFNAIKNENKNDADFFKIDYSKYV-FPEGINESFKGVIDSCLVEDPNGRISVQ 577
            YLAPE+ +N  +N KADVWS G++L EI+   K F+ + +K          K+    Y   P+  +  FK V+D  LV DPN R SV+
Sbjct:  190 YLAPELWNNLRYNKKADVWSLGVLLYEIMGMKKPFSASNLK------GLMSKVLAGTYAPLPDSFSSEFKRVVDGILVADPNDRPSVR 271

[Close]

BLAST of SMED30020735 vs. UniProt/SwissProt
Match: sp|Q12469|SKM1_YEAST (Serine/threonine-protein kinase SKM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKM1 PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.440e-7
Identity = 35/110 (31.82%), Postives = 54/110 (49.09%), Query Frame = -2

Query:  269 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDY-------SKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQF 577
            ++APEI++ K ++ K DVWS GI+L+E+I G+    N          D  K  Y        K   PE +++  K  +D+CL  +   R SV+ LL+ + L     P Q 
Sbjct:  548 WMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLN---------EDPLKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNVESRASVRKLLTFEFLSMACSPEQL 648

[Close]

BLAST of SMED30020735 vs. UniProt/SwissProt
Match: sp|Q54B33|PAKE_DICDI (Serine/threonine-protein kinase pakE OS=Dictyostelium discoideum OX=44689 GN=pakE PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.491e-7
Identity = 33/94 (35.11%), Postives = 53/94 (56.38%), Query Frame = -2

Query:  296 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLL 577
            Y+APE++   P+N KAD+WS GI L+E+  G+  N   +++ N+   +   +   K   P+  +  F   + +CLV+DP  R SV  LLS D +
Sbjct:  812 YMAPEVILKAPYNSKADIWSLGITLIELAEGRPPN-RGLRSMNQL-VEIPNMPPPKLSNPKDWSPCFNNFLATCLVKDPVQRPSVIDLLSHDFI 903

[Close]

BLAST of SMED30020735 vs. UniProt/SwissProt
Match: sp|Q54WX4|AURK_DICDI (Aurora kinase OS=Dictyostelium discoideum OX=44689 GN=aurK PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.520e-7
Identity = 30/87 (34.48%), Postives = 50/87 (57.47%), Query Frame = -2

Query:  320 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQ 580
            +YL PE+++ K ++  ADVWS GI++ E + G+   F +  +E KN   F  I  +   +P  I+   K +I   LV DP+ RI+++
Sbjct:  271 EYLPPEVIEKKGYDQTADVWSLGILIFEFLVGRS-PFTS--DEEKN--IFHNIQENDVYYPSSISPEAKDLISRLLVSDPHQRITLK 352

[Close]

BLAST of SMED30020735 vs. TrEMBL
Match: A0A397SKV7 (Kinase-like domain-containing protein OS=Glomus cerebriforme OX=658196 GN=C1645_727773 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 5.267e-9
Identity = 33/111 (29.73%), Postives = 62/111 (55.86%), Query Frame = -2

Query:  254 NKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMN 586
             KDYL PE+++N+P + K D+WS G++  E++TG   N    ++ N  D  + +I   ++  P+ ++   + +I S L  DPN R+ ++ +L+   ++   +PS  L   N
Sbjct:  178 TKDYLPPEMVENQPHDEKIDLWSLGVLCYELLTG---NAPFEESANGRDTTYKRIAKVEFTIPDHVSADARDLIGSLLQRDPNKRLPIKDVLNHPWILKHQKPSNVLRTTN 285

[Close]

BLAST of SMED30020735 vs. TrEMBL
Match: A0A165GMC0 (Kinase-like protein (Fragment) OS=Calocera cornea HHB12733 OX=1353952 GN=CALCODRAFT_451934 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.318e-8
Identity = 32/95 (33.68%), Postives = 53/95 (55.79%), Query Frame = -2

Query:  311 NKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNE---NKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 586
               ++APE++  + ++  AD+WSFGI+ LEI  G+  N     N+   +   +    +D +  V P    +SFK V+D+CL +DP+ R + Q LL
Sbjct:  289 TPSWMAPEVVRRQAYDSAADIWSFGILCLEITRGRAPNSREPPNKILLHTLSSPPPTLDLTGGVHP--YTQSFKDVVDACLQKDPSARPTAQQLL 381

[Close]

BLAST of SMED30020735 vs. TrEMBL
Match: T0PWX7 (NEK protein kinase OS=Saprolegnia diclina (strain VS20) OX=1156394 GN=SDRG_16619 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.562e-8
Identity = 34/96 (35.42%), Postives = 53/96 (55.21%), Query Frame = -2

Query:  296 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKY--VFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLL 577
            Y+APEI + +P+ +KADVW+ G IL E++TG+C  F A    +   A   KI    Y  + P       + +I + L  DPN R S++ +L++  L
Sbjct:  170 YMAPEIFEGQPYGLKADVWALGCILYELMTGRC-PFRA----SSYPALVHKISAGDYDALAPTTCRLPTRHLIAAMLAHDPNDRPSIESILTLPFL 260

[Close]

BLAST of SMED30020735 vs. TrEMBL
Match: A0A2Z6QCU0 (Protein kinase domain-containing protein OS=Rhizophagus clarus OX=94130 GN=RclHR1_14470008 PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.360e-8
Identity = 33/111 (29.73%), Postives = 61/111 (54.95%), Query Frame = -2

Query:  254 NKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMN 586
             KDYL PE+++N+P + K D+WS G++  E++TG   N    ++ N  D  + +I    +  P+ ++   + +I S L  DPN R+ ++ +L+   ++   + S  L M N
Sbjct:  199 TKDYLPPEMVENQPHDEKIDLWSLGVLCYELLTG---NAPFEESANGRDTTYKRIAKVDFTIPDHVSADARDLISSLLQRDPNKRLPIKDVLNHPWIVKHQKNSNVLQMTN 306

[Close]

BLAST of SMED30020735 vs. TrEMBL
Match: K2N952 (Protein kinase, putative,serine/threonine-protein kinase Nek1, putative OS=Trypanosoma cruzi marinkellei OX=85056 GN=MOQ_004816 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 5.142e-8
Identity = 33/95 (34.74%), Postives = 57/95 (60.00%), Query Frame = -2

Query:  296 YLAPEILDNKPWNIKADVWSFGIILLEIITGK-CFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLL 577
            Y +PEI  NKP+N K+DVW+ G++L E+ TGK  F+ N+++   +      K  Y+    P   +  F+ ++D CL +DP+ R S++  LS+ ++
Sbjct:  168 YFSPEICRNKPYNNKSDVWALGVLLYELATGKHPFDGNSMQQLMQR---IVKGSYAP--LPSHFSPEFRKMVDWCLQKDPSRRPSIRQTLSLPII 257

[Close]

BLAST of SMED30020735 vs. Ensembl Cavefish
Match: aurka (aurora kinase C-like [Source:NCBI gene;Acc:103034578])

HSP 1 Score: 52.373 bits (124), Expect = 7.370e-8
Identity = 25/91 (27.47%), Postives = 49/91 (53.85%), Query Frame = -2

Query:  308 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLS 580
            DYL PE+++ K  + K D+WS G++  E + G+   F    +E      + KI   ++ +P  ++E  + +I+  L  +P  R+ +Q +L+
Sbjct:  315 DYLPPEMIEGKTHDEKVDLWSLGVLCYEFLVGRP-PFETKSHEE----TYRKISRVEFTYPAHVSEGSRNLINGLLKHNPMHRLPIQGVLT 400

[Close]

BLAST of SMED30020735 vs. Ensembl Cavefish
Match: aurka (aurora kinase C-like [Source:NCBI gene;Acc:103034578])

HSP 1 Score: 51.9878 bits (123), Expect = 7.493e-8
Identity = 25/91 (27.47%), Postives = 49/91 (53.85%), Query Frame = -2

Query:  308 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLS 580
            DYL PE+++ K  + K D+WS G++  E + G+   F    +E      + KI   ++ +P  ++E  + +I+  L  +P  R+ +Q +L+
Sbjct:  269 DYLPPEMIEGKTHDEKVDLWSLGVLCYEFLVGRP-PFETKSHEE----TYRKISRVEFTYPAHVSEGSRNLINGLLKHNPMHRLPIQGVLT 354

[Close]

BLAST of SMED30020735 vs. Ensembl Cavefish
Match: plk1 (polo like kinase 1 [Source:NCBI gene;Acc:103034005])

HSP 1 Score: 50.0618 bits (118), Expect = 3.903e-7
Identity = 32/98 (32.65%), Postives = 48/98 (48.98%), Query Frame = -2

Query:  290 DYLAPEILDNKPWNIKADVWSFGIILLEIITGK-CFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 580
            +Y+APE+L  K  + + DVWS G IL  ++ GK  F  + +K        + +I  ++Y  P  IN     +I   L  DP  R SV  LL+ +   S
Sbjct:  201 NYIAPEVLCKKGHSFEVDVWSLGCILYTLLVGKPPFETSCLKE------TYVRIKKNEYSIPRQINPVAASLIRRMLHADPTLRPSVADLLTDEFFTS 292

[Close]

BLAST of SMED30020735 vs. Ensembl Cavefish
Match: stk26 (serine/threonine kinase 26 [Source:NCBI gene;Acc:103024322])

HSP 1 Score: 49.6766 bits (117), Expect = 5.594e-7
Identity = 31/109 (28.44%), Postives = 53/109 (48.62%), Query Frame = -2

Query:  266 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFN-----FNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFL 577
            ++APE++    ++ KAD+WS GI  +E+  G+  N        + +  KN       D+SK         SFK  +DSCL +DP  R + + LL    ++   + + +L
Sbjct:  185 WMAPEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFHIPKNTPPTLNGDFSK---------SFKEFVDSCLNKDPTFRPTAKELLKHKFIVKNAKKTSYL 284

[Close]

BLAST of SMED30020735 vs. Ensembl Cavefish
Match: stk40 (serine/threonine kinase 40 [Source:NCBI gene;Acc:103034368])

HSP 1 Score: 49.2914 bits (116), Expect = 6.891e-7
Identity = 27/92 (29.35%), Postives = 53/92 (57.61%), Query Frame = -2

Query:  311 YLAPEILDNKPWNIK-ADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPE--GINESFKGVIDSCLVEDPNGRISVQMLL 577
            Y++P++L ++P+  K +D+W+ G++L  ++ G+   +++I  E      F KI  ++Y  PE   ++ES   +I   LV DP  R++   +L
Sbjct:  240 YISPDVLSSRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQEL-----FRKIKAAEYSIPEDGRVSESTVCLIRKLLVLDPQQRLTAAEVL 326

[Close]

BLAST of SMED30020735 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007414.1 (pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1 gene:ENSPMAG00000006689.1 transcript:ENSPMAT00000007414.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 1.450e-8
Identity = 30/96 (31.25%), Postives = 48/96 (50.00%), Query Frame = -2

Query:  290 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 577
            YL+PEI +NKP+N K+D+W+ G +L E+ T +    +A +  N  +     I  S        +   +  +   L  DP  R  V +LLS  LL++
Sbjct:  169 YLSPEICENKPYNHKSDMWALGCVLYEMCTLR----HAFEAGNMKNLVLKIIRGSFPPVSAHYSSELRSTLSGLLRRDPRARPPVAVLLSRPLLMA 260

[Close]

BLAST of SMED30020735 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010251.1 (pep scaffold:Pmarinus_7.0:GL485493:5407:10850:-1 gene:ENSPMAG00000009279.1 transcript:ENSPMAT00000010251.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 1.305e-7
Identity = 30/97 (30.93%), Postives = 52/97 (53.61%), Query Frame = -2

Query:  290 YLAPEILDNKPW-NIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 577
            Y APE++  KP+   +AD+WS G++L  ++ G    F    ++N   A + KI   KY  P+ ++     ++D  +  DP  R++V+ LL    L+S
Sbjct:   39 YAAPELIQGKPYCGSEADLWSMGVLLYALLCG----FLPFDDDNVM-ALYRKIQRGKYEIPKWLSRESITLLDQLVQVDPTRRLAVRHLLGHPWLMS 130

[Close]

BLAST of SMED30020735 vs. Ensembl Sea Lamprey
Match: stk25a (serine/threonine kinase 25a [Source:ZFIN;Acc:ZDB-GENE-041010-92])

HSP 1 Score: 48.521 bits (114), Expect = 2.823e-7
Identity = 29/94 (30.85%), Postives = 47/94 (50.00%), Query Frame = -2

Query:  311 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNE-----NKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 577
            ++APE++    ++ KAD+WS GI  +E+  G+  N             KN+    + +YSK         +FK  +DSCL +DP  R + + LL
Sbjct:  189 WMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSELHPMRVLFLIPKNNPPTLEGNYSK---------AFKEFVDSCLNKDPQFRPTAKELL 273

[Close]

BLAST of SMED30020735 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008103.1 (pep scaffold:Pmarinus_7.0:GL476484:390774:392599:-1 gene:ENSPMAG00000007328.1 transcript:ENSPMAT00000008103.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.595 bits (109), Expect = 3.220e-7
Identity = 31/113 (27.43%), Postives = 53/113 (46.90%), Query Frame = -2

Query:  254 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFN-----AIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMN 577
            ++APE++   P+  + D+WS G++++E++ G+   FN     A++    N     KI  S     +G     +  +D  LV DP  R S   LL    L +   PS  + +M 
Sbjct:   29 WMAPEVISRLPYGPEVDIWSLGVMVMEMVDGEPPYFNEPPLQAMRRIRDNIPP--KIKNS-----QGTTVQLRSFLDRMLVRDPTQRASAVELLGHPFLQNAGPPSCVIPLMR 134

[Close]

BLAST of SMED30020735 vs. Ensembl Sea Lamprey
Match: hck (HCK proto-oncogene, Src family tyrosine kinase [Source:ZFIN;Acc:ZDB-GENE-090313-72])

HSP 1 Score: 47.3654 bits (111), Expect = 6.062e-7
Identity = 30/93 (32.26%), Postives = 46/93 (49.46%), Query Frame = -2

Query:  305 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVF--PEGINESFKGVIDSCLVEDPNGRISVQMLLSI 577
            + APE ++   ++IK+DVWSFGI+L EIIT     F  I     +++D  +     Y    PE   +   GV+  C  E P  R + + L  +
Sbjct:  343 WTAPEAINYGSFSIKSDVWSFGILLTEIIT-----FGRIPYPGMSNSDVIRQLERGYRMPCPEQAPKQLYGVMMDCWKERPEERPTFEYLQGV 430

[Close]

BLAST of SMED30020735 vs. Ensembl Yeast
Match: SKM1 (Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication [Source:SGD;Acc:S000005473])

HSP 1 Score: 54.299 bits (129), Expect = 2.074e-9
Identity = 35/110 (31.82%), Postives = 54/110 (49.09%), Query Frame = -2

Query:  269 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDY-------SKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQF 577
            ++APEI++ K ++ K DVWS GI+L+E+I G+    N          D  K  Y        K   PE +++  K  +D+CL  +   R SV+ LL+ + L     P Q 
Sbjct:  548 WMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLN---------EDPLKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNVESRASVRKLLTFEFLSMACSPEQL 648

[Close]

BLAST of SMED30020735 vs. Ensembl Yeast
Match: PKH1 (Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication [Source:SGD;Acc:S000002898])

HSP 1 Score: 50.8322 bits (120), Expect = 3.478e-8
Identity = 26/89 (29.21%), Postives = 50/89 (56.18%), Query Frame = -2

Query:  314 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQML 580
            +Y++PE+L++   + + D+W+FG IL +++ GK       K  N+    F K+   +Y F  G  +  K ++   LV DPN R++++ +
Sbjct:  302 EYVSPELLNDNYTDSRCDIWAFGCILYQMLAGK----PPFKAANEY-LTFQKVMKIQYAFTAGFPQIVKDLVKKLLVRDPNDRLTIKQI 385

[Close]

BLAST of SMED30020735 vs. Ensembl Yeast
Match: TPK1 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication [Source:SGD;Acc:S000003700])

HSP 1 Score: 49.6766 bits (117), Expect = 6.437e-8
Identity = 27/86 (31.40%), Postives = 46/86 (53.49%), Query Frame = -2

Query:  329 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKC--FNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRI 580
            DY+APE++  KP+N   D WSFGI++ E++ G    ++ N +K        + KI  ++  FP   NE  K ++   +  D + R+
Sbjct:  247 DYIAPEVVSTKPYNKSIDWWSFGILIYEMLAGYTPFYDSNTMKT-------YEKILNAELRFPPFFNEDVKDLLSRLITRDLSQRL 325

[Close]

BLAST of SMED30020735 vs. Ensembl Yeast
Match: HSL1 (Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p [Source:SGD;Acc:S000001584])

HSP 1 Score: 50.0618 bits (118), Expect = 7.562e-8
Identity = 32/96 (33.33%), Postives = 50/96 (52.08%), Query Frame = -2

Query:  296 YLAPEILDNKPWNI-KADVWSFGIILLEIITGKC-FNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLL 577
            Y +PEI+  +P++   +DVWS GI+L  ++TG   FN + IK          K+   KY  P  ++   + +I   LV DP  RI+ Q +L   L+
Sbjct:  280 YASPEIVMGRPYHGGPSDVWSCGIVLFALLTGHLPFNDDNIKKL------LLKVQSGKYQMPSNLSSEARDLISKILVIDPEKRITTQEILKHPLI 369

[Close]

BLAST of SMED30020735 vs. Ensembl Yeast
Match: KCC4 (Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication [Source:SGD;Acc:S000000529])

HSP 1 Score: 47.7506 bits (112), Expect = 3.904e-7
Identity = 33/98 (33.67%), Postives = 49/98 (50.00%), Query Frame = -2

Query:  296 YLAPEILDNKPW-NIKADVWSFGIILLEIITGKCFNFNAIKNENKNDAD-FFKIDYSKYVFP--EGINESFKGVIDSCLVEDPNGRISVQMLLSIDLL 577
            Y APEI+   P+    +DVWS G+IL  ++TG+         EN N  D   K+   ++  P    I+   + +I   LV DP  RI ++ +LS  LL
Sbjct:  192 YAAPEIVSGLPYEGFASDVWSCGVILFALLTGRL----PFDEENGNVRDLLLKVQKGQFEMPNDTEISRDAQDLIGKILVVDPRQRIKIRDILSHPLL 285

[Close]

BLAST of SMED30020735 vs. Ensembl Nematostella
Match: EDO35699 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKU3])

HSP 1 Score: 50.8322 bits (120), Expect = 1.015e-7
Identity = 31/96 (32.29%), Postives = 54/96 (56.25%), Query Frame = -2

Query:  290 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 577
            Y++PEI+  K +N K+D+W+ G +L E++T K   F+A  N  +  +D  K  Y +    E   E    +++  L ++P+ R SVQ LL + +L +
Sbjct:  180 YMSPEIVQGKKYNQKSDMWAVGCVLYEVLTLKRV-FDA-SNPLRLVSDIVKGHYEE--IDERYTEEMNSLVNKLLSQNPDDRPSVQELLEMPILAT 271

[Close]

BLAST of SMED30020735 vs. Ensembl Nematostella
Match: EDO49690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG02])

HSP 1 Score: 48.521 bits (114), Expect = 3.827e-7
Identity = 31/90 (34.44%), Postives = 46/90 (51.11%), Query Frame = -2

Query:  311 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKY-VFPEGINESFKGVIDSCLVEDPNGRISVQMLL 577
            Y++PE+  NKP+N K+DVW+ G  L E+ T +   FNA        +  +KI   K    P+  +     +I S L +DP+ R S   LL
Sbjct:  172 YMSPELFSNKPYNHKSDVWALGCCLYEMCTLR-HAFNA----KDMSSLVYKILKGKTPPLPKQYSTDLCSIIKSMLDQDPDKRPSASRLL 256

[Close]

BLAST of SMED30020735 vs. Ensembl Nematostella
Match: EDO35437 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLH0])

HSP 1 Score: 48.521 bits (114), Expect = 4.918e-7
Identity = 25/90 (27.78%), Postives = 46/90 (51.11%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE+++ K  + K D+WS G++  E + GK       + E  N+  + +I      FP  ++   + +I   L  +PN R+ ++ +L
Sbjct:  209 DYLPPEMIEGKEHDEKVDLWSIGVLCYEFLVGK----PPFEAEGHNET-YRRISRVDLRFPSHVSPGARDLISKLLQHNPNNRLPLKAVL 293

[Close]

BLAST of SMED30020735 vs. Ensembl Nematostella
Match: EDO49822 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG20])

HSP 1 Score: 48.1358 bits (113), Expect = 5.379e-7
Identity = 28/89 (31.46%), Postives = 48/89 (53.93%), Query Frame = -2

Query:  311 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLL 577
            YL+PEI++N+P+N K+D+WS G +L E++T K    +A +  N  +     I  S    P   +   + ++   L  +P+ R SV  +L
Sbjct:  169 YLSPEIVENRPYNNKSDIWSLGCVLYEMLTLK----HAFEAGNMKNLVLKIIRGSYPPIPLRYSADIRMLVAQLLKRNPHDRPSVNTVL 253

[Close]

BLAST of SMED30020735 vs. Ensembl Nematostella
Match: EDO46185 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQY7])

HSP 1 Score: 47.7506 bits (112), Expect = 1.088e-6
Identity = 30/97 (30.93%), Postives = 52/97 (53.61%), Query Frame = -2

Query:  290 YLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYV-FPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 577
            Y++PE+ + KP+N K+DVWS G +L E+ T K     A +  N   A   KI    +   PE  +E  + +I   LV DP  R  ++ +++  ++++
Sbjct:  169 YISPELCEGKPYNQKSDVWSLGCVLYELTTLK----RAFEASNL-PALVLKIMRGYFSPIPERYSEELRKLILDMLVLDPTKRPGIKEIMAQPVIVN 260

[Close]

BLAST of SMED30020735 vs. Ensembl Medaka
Match: si:ch211-1i11.3 (mitogen-activated protein kinase kinase kinase 5 [Source:NCBI gene;Acc:101167985])

HSP 1 Score: 53.1434 bits (126), Expect = 4.885e-8
Identity = 35/101 (34.65%), Postives = 52/101 (51.49%), Query Frame = -2

Query:  287 YLAPEILDNKP--WNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKY--VFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP 577
            Y+APEI+D  P  +   AD+WS G  ++E+ TGK   F+ + +     A  FK+   K     PE +++  KG I SC   +P+ R +   LL    L SP
Sbjct:  798 YMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKT-PFHELGSPQ---AAMFKVGMFKIHPKVPECMSDEAKGFIMSCFEPNPDNRATAAELLMDTFLKSP 894

[Close]

BLAST of SMED30020735 vs. Ensembl Medaka
Match: si:ch211-1i11.3 (mitogen-activated protein kinase kinase kinase 5 [Source:NCBI gene;Acc:101167985])

HSP 1 Score: 52.7582 bits (125), Expect = 5.393e-8
Identity = 35/101 (34.65%), Postives = 52/101 (51.49%), Query Frame = -2

Query:  287 YLAPEILDNKP--WNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKY--VFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP 577
            Y+APEI+D  P  +   AD+WS G  ++E+ TGK   F+ + +     A  FK+   K     PE +++  KG I SC   +P+ R +   LL    L SP
Sbjct:  742 YMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKT-PFHELGSPQ---AAMFKVGMFKIHPKVPECMSDEAKGFIMSCFEPNPDNRATAAELLMDTFLKSP 838

[Close]

BLAST of SMED30020735 vs. Ensembl Medaka
Match: aurka (aurora kinase A [Source:NCBI gene;Acc:101165631])

HSP 1 Score: 51.2174 bits (121), Expect = 1.767e-7
Identity = 28/92 (30.43%), Postives = 48/92 (52.17%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFP--EGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE+++ K  + K D+WS G++  E + GK   F A      ND  + +I   +Y +P    I++  K ++   L  +P  R+ +Q +L
Sbjct:  283 DYLPPEMIEGKTHDEKVDLWSLGVLCYEFLVGKP-PFEA----KTNDETYRRISRVEYTYPAHAAISDGAKDLVAKLLKHNPMHRLPIQGVL 369

[Close]

BLAST of SMED30020735 vs. Ensembl Medaka
Match: aurka (aurora kinase A [Source:NCBI gene;Acc:101165631])

HSP 1 Score: 51.2174 bits (121), Expect = 1.813e-7
Identity = 28/92 (30.43%), Postives = 48/92 (52.17%), Query Frame = -2

Query:  311 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFP--EGINESFKGVIDSCLVEDPNGRISVQMLL 580
            DYL PE+++ K  + K D+WS G++  E + GK   F A      ND  + +I   +Y +P    I++  K ++   L  +P  R+ +Q +L
Sbjct:  319 DYLPPEMIEGKTHDEKVDLWSLGVLCYEFLVGKP-PFEA----KTNDETYRRISRVEYTYPAHAAISDGAKDLVAKLLKHNPMHRLPIQGVL 405

[Close]

BLAST of SMED30020735 vs. Ensembl Medaka
Match: stk17al (serine/threonine-protein kinase 17A [Source:NCBI gene;Acc:101163818])

HSP 1 Score: 50.8322 bits (120), Expect = 2.068e-7
Identity = 34/110 (30.91%), Postives = 54/110 (49.09%), Query Frame = -2

Query:  251 DYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLISP*RPSQFLHMMNA 580
            +Y+APE+L  +P +   D+WS G++   ++TG+       K +   +     +DYS+  F EGI+      I S LV++P  R + +  L    L S     Q LHM  A
Sbjct:  191 EYVAPEVLSYEPISTATDMWSIGVLTYVMLTGESPFLGDEKQQTFLNISQVNVDYSQDTF-EGISSLAVDFIKSLLVKNPRKRATAEECLRHPWLNS----HQHLHMRPA 295

[Close]

BLAST of SMED30020735 vs. Planmine SMEST
Match: SMESG000003887.1 (SMESG000003887.1)

HSP 1 Score: 179.874 bits (455), Expect = 1.077e-52
Identity = 85/93 (91.40%), Postives = 90/93 (96.77%), Query Frame = -2

Query:  317 NEINKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQM 595
            ++IN+ YLAPEI + KPWNIKADVWSFG+ILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQM
Sbjct:  642 DDINRMYLAPEIRNGKPWNIKADVWSFGMILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQM 734

[Close]

BLAST of SMED30020735 vs. Planmine SMEST
Match: SMESG000003887.1 (SMESG000003887.1)

HSP 1 Score: 179.874 bits (455), Expect = 1.246e-52
Identity = 85/93 (91.40%), Postives = 90/93 (96.77%), Query Frame = -2

Query:  317 NEINKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQM 595
            ++IN+ YLAPEI + KPWNIKADVWSFG+ILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQM
Sbjct:  658 DDINRMYLAPEIRNGKPWNIKADVWSFGMILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQM 750

[Close]

BLAST of SMED30020735 vs. Planmine SMEST
Match: SMESG000003867.1 (SMESG000003867.1)

HSP 1 Score: 157.918 bits (398), Expect = 1.486e-46
Identity = 78/100 (78.00%), Postives = 84/100 (84.00%), Query Frame = -2

Query:  290 INKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 589
            IN  YLAPEIL  KPWNIKADVWSFG+ILLEIITGKCFNF+A K E  ND++FFKIDY+KY+FP  INE FKGVID CLVEDPN RISV  LLSIDLL S
Sbjct:  300 INGKYLAPEILKGKPWNIKADVWSFGVILLEIITGKCFNFSA-KKEALNDSNFFKIDYTKYIFPRSINERFKGVIDRCLVEDPNYRISVHELLSIDLLRS 398

[Close]

BLAST of SMED30020735 vs. Planmine SMEST
Match: SMESG000003867.1 (SMESG000003867.1)

HSP 1 Score: 158.303 bits (399), Expect = 3.937e-46
Identity = 78/100 (78.00%), Postives = 84/100 (84.00%), Query Frame = -2

Query:  290 INKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 589
            IN  YLAPEIL  KPWNIKADVWSFG+ILLEIITGKCFNF+A K E  ND++FFKIDY+KY+FP  INE FKGVID CLVEDPN RISV  LLSIDLL S
Sbjct:  379 INGKYLAPEILKGKPWNIKADVWSFGVILLEIITGKCFNFSA-KKEALNDSNFFKIDYTKYIFPRSINERFKGVIDRCLVEDPNYRISVHELLSIDLLRS 477

[Close]

BLAST of SMED30020735 vs. Planmine SMEST
Match: SMESG000003867.1 (SMESG000003867.1)

HSP 1 Score: 158.303 bits (399), Expect = 4.003e-46
Identity = 78/100 (78.00%), Postives = 84/100 (84.00%), Query Frame = -2

Query:  290 INKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENKNDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLLIS 589
            IN  YLAPEIL  KPWNIKADVWSFG+ILLEIITGKCFNF+A K E  ND++FFKIDY+KY+FP  INE FKGVID CLVEDPN RISV  LLSIDLL S
Sbjct:  380 INGKYLAPEILKGKPWNIKADVWSFGVILLEIITGKCFNFSA-KKEALNDSNFFKIDYTKYIFPRSINERFKGVIDRCLVEDPNYRISVHELLSIDLLRS 478

The following BLAST results are available for this feature:

BLAST of SMED30020735 vs. Ensembl Human
BLAST of SMED30020735 vs. Ensembl Celegans
BLAST of SMED30020735 vs. Ensembl Fly
BLAST of SMED30020735 vs. Ensembl Zebrafish
BLAST of SMED30020735 vs. Ensembl Xenopus
BLAST of SMED30020735 vs. Ensembl Mouse
BLAST of SMED30020735 vs. UniProt/SwissProt
BLAST of SMED30020735 vs. TrEMBL
BLAST of SMED30020735 vs. Ensembl Cavefish
BLAST of SMED30020735 vs. Ensembl Sea Lamprey
BLAST of SMED30020735 vs. Ensembl Yeast
BLAST of SMED30020735 vs. Ensembl Nematostella
BLAST of SMED30020735 vs. Ensembl Medaka
BLAST of SMED30020735 vs. Planmine SMEST

BLAST of SMED30020735 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
PLK1	1.754e-7	28.57	polo like kinase 1 [Source:HGNC Symbol;Acc:HGNC:90...	[more]
PAK4	2.575e-7	27.43	p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;...	[more]
PAK4	1.342e-6	27.43	p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;...	[more]
PAK4	1.342e-6	27.43	p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;...	[more]
PAK4	1.553e-6	27.43	p21 (RAC1) activated kinase 4 [Source:HGNC Symbol;...	[more]

BLAST of SMED30020735 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
T21G5.1	2.338e-7	31.76	Tyrosine-protein kinase [Source:UniProtKB/TrEMBL;...	[more]
ZK524.4	9.354e-7	32.61	pep chromosome:WBcel235:I:7460858:7466424:-1 gene:...	[more]
air-1	1.070e-6	25.51	Aurora/Ipl1 Related kinase; Aurora/Ipl1-related pr...	[more]
air-1	1.070e-6	25.51	Aurora/Ipl1 Related kinase; Aurora/Ipl1-related pr...	[more]
pkc-3	1.274e-6	25.27	Protein kinase C-like 3 [Source:UniProtKB/Swiss-P...	[more]

BLAST of SMED30020735 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
aurA	2.599e-8	28.89	gene:FBgn0000147 transcript:FBtr0082483	[more]
Ret	9.355e-8	32.22	gene:FBgn0011829 transcript:FBtr0081493	[more]
Ret	9.355e-8	32.22	gene:FBgn0011829 transcript:FBtr0081494	[more]
Ret	9.355e-8	32.22	gene:FBgn0011829 transcript:FBtr0081495	[more]
inaC	9.721e-8	34.12	gene:FBgn0004784 transcript:FBtr0087047	[more]

BLAST of SMED30020735 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
stk17al	8.128e-9	33.33	serine/threonine kinase 17a like [Source:ZFIN;Acc:...	[more]
pak4	6.279e-7	29.46	p21 protein (Cdc42/Rac)-activated kinase 4 [Source...	[more]
plk1	1.015e-6	31.63	polo-like kinase 1 (Drosophila) [Source:ZFIN;Acc:Z...	[more]
plk1	1.015e-6	31.63	polo-like kinase 1 (Drosophila) [Source:ZFIN;Acc:Z...	[more]
stk40	1.687e-6	29.00	serine/threonine kinase 40 [Source:ZFIN;Acc:ZDB-GE...	[more]

BLAST of SMED30020735 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSXETT00000027062.1	2.775e-7	27.38	pep primary_assembly:Xenopus_tropicalis_v9.1:2:269...	[more]
stk40	1.529e-6	29.35	serine/threonine kinase 40 [Source:NCBI gene;Acc:1...	[more]
sap130	1.598e-6	29.47	Sin3A associated protein 130kDa [Source:Xenbase;Ac...	[more]
stk40	1.720e-6	29.35	serine/threonine kinase 40 [Source:NCBI gene;Acc:1...	[more]
ENSXETT00000029423.1	1.831e-6	32.22	serine/threonine-protein kinase 17A-like [Source:N...	[more]

BLAST of SMED30020735 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Plk1	1.036e-7	28.57	polo like kinase 1 [Source:MGI Symbol;Acc:MGI:9762...	[more]
Aurkc	1.108e-6	26.67	aurora kinase C [Source:MGI Symbol;Acc:MGI:1321119...	[more]
Aurkc	1.824e-6	26.67	aurora kinase C [Source:MGI Symbol;Acc:MGI:1321119...	[more]
Aurkc	1.824e-6	26.67	aurora kinase C [Source:MGI Symbol;Acc:MGI:1321119...	[more]
Aurkc	2.153e-6	26.67	aurora kinase C [Source:MGI Symbol;Acc:MGI:1321119...	[more]

BLAST of SMED30020735 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q08942\|NRKA_TRYBB	1.745e-9	39.78	Putative serine/threonine-protein kinase A OS=Tryp...	[more]
sp\|Q03428\|NRKB_TRYBB	3.737e-8	39.77	Putative serine/threonine-protein kinase B OS=Tryp...	[more]
sp\|Q12469\|SKM1_YEAST	1.440e-7	31.82	Serine/threonine-protein kinase SKM1 OS=Saccharomy...	[more]
sp\|Q54B33\|PAKE_DICDI	1.491e-7	35.11	Serine/threonine-protein kinase pakE OS=Dictyostel...	[more]
sp\|Q54WX4\|AURK_DICDI	1.520e-7	34.48	Aurora kinase OS=Dictyostelium discoideum OX=44689...	[more]

BLAST of SMED30020735 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A397SKV7	5.267e-9	29.73	Kinase-like domain-containing protein OS=Glomus ce...	[more]
A0A165GMC0	1.318e-8	33.68	Kinase-like protein (Fragment) OS=Calocera cornea ...	[more]
T0PWX7	1.562e-8	35.42	NEK protein kinase OS=Saprolegnia diclina (strain ...	[more]
A0A2Z6QCU0	2.360e-8	29.73	Protein kinase domain-containing protein OS=Rhizop...	[more]
K2N952	5.142e-8	34.74	Protein kinase, putative,serine/threonine-protein ...	[more]

BLAST of SMED30020735 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
aurka	7.370e-8	27.47	aurora kinase C-like [Source:NCBI gene;Acc:1030345...	[more]
aurka	7.493e-8	27.47	aurora kinase C-like [Source:NCBI gene;Acc:1030345...	[more]
plk1	3.903e-7	32.65	polo like kinase 1 [Source:NCBI gene;Acc:103034005...	[more]
stk26	5.594e-7	28.44	serine/threonine kinase 26 [Source:NCBI gene;Acc:1...	[more]
stk40	6.891e-7	29.35	serine/threonine kinase 40 [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30020735 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000007414.1	1.450e-8	31.25	pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1...	[more]
ENSPMAT00000010251.1	1.305e-7	30.93	pep scaffold:Pmarinus_7.0:GL485493:5407:10850:-1 g...	[more]
stk25a	2.823e-7	30.85	serine/threonine kinase 25a [Source:ZFIN;Acc:ZDB-G...	[more]
ENSPMAT00000008103.1	3.220e-7	27.43	pep scaffold:Pmarinus_7.0:GL476484:390774:392599:-...	[more]
hck	6.062e-7	32.26	HCK proto-oncogene, Src family tyrosine kinase [So...	[more]

BLAST of SMED30020735 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
SKM1	2.074e-9	31.82	Member of the PAK family of serine/threonine prote...	[more]
PKH1	3.478e-8	29.21	Serine/threonine protein kinase; involved in sphin...	[more]
TPK1	6.437e-8	31.40	cAMP-dependent protein kinase catalytic subunit; p...	[more]
HSL1	7.562e-8	33.33	Nim1p-related protein kinase; septin-binding kinas...	[more]
KCC4	3.904e-7	33.67	Protein kinase of the bud neck involved in the sep...	[more]

BLAST of SMED30020735 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO35699	1.015e-7	32.29	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49690	3.827e-7	34.44	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35437	4.918e-7	27.78	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49822	5.379e-7	31.46	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46185	1.088e-6	30.93	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30020735 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
si:ch211-1i11.3	4.885e-8	34.65	mitogen-activated protein kinase kinase kinase 5 [...	[more]
si:ch211-1i11.3	5.393e-8	34.65	mitogen-activated protein kinase kinase kinase 5 [...	[more]
aurka	1.767e-7	30.43	aurora kinase A [Source:NCBI gene;Acc:101165631]	[more]
aurka	1.813e-7	30.43	aurora kinase A [Source:NCBI gene;Acc:101165631]	[more]
stk17al	2.068e-7	30.91	serine/threonine-protein kinase 17A [Source:NCBI g...	[more]

BLAST of SMED30020735 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000003887.1	1.077e-52	91.40	SMESG000003887.1	[more]
SMESG000003887.1	1.246e-52	91.40	SMESG000003887.1	[more]
SMESG000003867.1	1.486e-46	78.00	SMESG000003867.1	[more]
SMESG000003867.1	3.937e-46	78.00	SMESG000003867.1	[more]
SMESG000003867.1	4.003e-46	78.00	SMESG000003867.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30020735 ID=SMED30020735|Name=SMED30020735|organism=Schmidtea mediterranea sexual|type=transcript|length=596bp
TTTTTTTTTAAAAAGAATTAATTTTTCCTTCTAGCGAACTTAAAAGACAA
CGCAAACCTTTGATATAGTTCTCCATCTGATCATACATATGAAAGTCCGT
ATCACCAAGGAAATGGCAAGAACAGTTACAATTATTCGAATTTTTGAAAG
GTTCAATTAAAGATAATAAATTATTCATAATCAAAGATAAAACAACACAA
ATAATAATAGCAAATAATAAATATTTTGACCATAATTAATAAAACCAGAC
GGCGTTCATCATATGCAAGAATTGCGAGGGCCTTCAAGGGGAAATTAGTA
AATCAATGCTCAACAACATTTGCACCGATATCCTACCATTTGGATCTTCA
ACCAAGCAGCTGTCAATGACACCCTTGAAACTTTCATTTATTCCTTCCGG
AAAAACATATTTGCTATAATCAATTTTAAAGAAATCAGCATCATTTTTAT
TTTCATTTTTGATGGCATTAAAATTAAAACATTTCCCGGTAATAATCTCC
AGCAGAATGATTCCAAATGACCACACGTCGGCCTTAATGTTCCACGGTTT
ATTGTCCAAAATCTCAGGAGCAAGATAATCTTTATTTATTTCATTT

protein sequence of SMED30020735-orf-1

>SMED30020735-orf-1 ID=SMED30020735-orf-1|Name=SMED30020735-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=104bp
NEINKDYLAPEILDNKPWNIKADVWSFGIILLEIITGKCFNFNAIKNENK
NDADFFKIDYSKYVFPEGINESFKGVIDSCLVEDPNGRISVQMLLSIDLL
ISP*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000070	intestinal phagocyte
PLANA:0003116	parenchymal cell
PLANA:0003117	parapharyngeal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR001245	Ser-Thr/Tyr_kinase_cat_dom
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf

Vocabulary: **biological process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **molecular function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 2..36 e-value: 1.0E-10 score: 43.1
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	Pkinase_Tyr	coord: 4..36 e-value: 2.4E-8 score: 33.6
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..36 score: 11.411
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	SSF56112	Protein kinase-like (PK-like)	coord: 6..36