Protein LKAAEAR1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020711 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of Protein LKAAEAR1 vs. Ensembl Human
Match: LKAAEAR1 (LKAAEAR motif containing 1 [Source:HGNC Symbol;Acc:HGNC:33718]) HSP 1 Score: 55.4546 bits (132), Expect = 2.752e-9 Identity = 38/87 (43.68%), Postives = 50/87 (57.47%), Query Frame = 1 Query: 208 LFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPT--KPQRETHKDALTKSQRERVEMILE---NDSILKR 453 L G LKAAE+R R+R +RL+Y + EI L+ Q SA A+RL+ LP KP R D L + +R RVE ILE + +I R Sbjct: 110 LLGVLKAAEARGRVRALRLRYTRMRAEEIALLIQRQKSARAAIRLELFLPPQLKPAR--IPDPLDRQERRRVETILEENVDGTIFPR 194
BLAST of Protein LKAAEAR1 vs. Ensembl Mouse
Match: Lkaaear1 (LKAAEAR motif containing 1 (IKAAEAR murine motif) [Source:MGI Symbol;Acc:MGI:2685538]) HSP 1 Score: 53.1434 bits (126), Expect = 1.372e-8 Identity = 30/77 (38.96%), Postives = 44/77 (57.14%), Query Frame = 1 Query: 208 LFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILEND 438 G +KAAE+R R+ +RL+Y + EI L+ Q SA A+RL+ LP + + D L + +R RVE ILE + Sbjct: 114 FLGLIKAAEARGRVHTLRLRYTRMRAEEISLLIQKQSSARAAIRLELFLPPQLKPTKIPDPLDRHERRRVETILEEE 190
BLAST of Protein LKAAEAR1 vs. UniProt/SwissProt
Match: sp|Q8TD35|LKAM1_HUMAN (Protein LKAAEAR1 OS=Homo sapiens OX=9606 GN=LKAAEAR1 PE=1 SV=3) HSP 1 Score: 55.4546 bits (132), Expect = 1.322e-8 Identity = 38/87 (43.68%), Postives = 50/87 (57.47%), Query Frame = 1 Query: 208 LFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPT--KPQRETHKDALTKSQRERVEMILE---NDSILKR 453 L G LKAAE+R R+R +RL+Y + EI L+ Q SA A+RL+ LP KP R D L + +R RVE ILE + +I R Sbjct: 110 LLGVLKAAEARGRVRALRLRYTRMRAEEIALLIQRQKSARAAIRLELFLPPQLKPAR--IPDPLDRQERRRVETILEENVDGTIFPR 194
BLAST of Protein LKAAEAR1 vs. UniProt/SwissProt
Match: sp|Q8BIG2|LKAM1_MOUSE (Protein LKAAEAR1 OS=Mus musculus OX=10090 GN=Lkaaear1 PE=2 SV=2) HSP 1 Score: 53.1434 bits (126), Expect = 9.598e-8 Identity = 30/77 (38.96%), Postives = 44/77 (57.14%), Query Frame = 1 Query: 208 LFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILEND 438 G +KAAE+R R+ +RL+Y + EI L+ Q SA A+RL+ LP + + D L + +R RVE ILE + Sbjct: 114 FLGLIKAAEARGRVHTLRLRYTRMRAEEISLLIQKQSSARAAIRLELFLPPQLKPTKIPDPLDRHERRRVETILEEE 190
BLAST of Protein LKAAEAR1 vs. TrEMBL
Match: K1S606 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10010069 PE=4 SV=1) HSP 1 Score: 118.627 bits (296), Expect = 7.194e-31 Identity = 61/122 (50.00%), Postives = 86/122 (70.49%), Query Frame = 1 Query: 70 YTNPSKEVLEAQQNSKKRILDQIKAQKKFRIVEDENSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILEN 435 Y +P KE+ +A +KKR+L++ + + E +E+ KH L GQLKAAE+RNR+RIMRL+YQ N EI HL++CQP+AL+AVRLQAL+P+ P+RE +D L K +R RVE +LE+ Sbjct: 12 YEDPPKEISDAMGATKKRLLEKRRVMTRINQDLPEEEIDERDKHAKLIGQLKAAEARNRLRIMRLRYQANRAQEISHLIACQPTALKAVRLQALVPSYPEREDRRDKLDKLERVRVERLLED 133
BLAST of Protein LKAAEAR1 vs. TrEMBL
Match: A0A210PR04 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT23666 PE=4 SV=1) HSP 1 Score: 116.701 bits (291), Expect = 7.153e-30 Identity = 62/126 (49.21%), Postives = 83/126 (65.87%), Query Frame = 1 Query: 70 YTNPSKEVLEAQQNSKKRILDQIKAQKKFRIVEDENSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILENDSIL 447 Y +P+KE+ EAQ + KR+LD+ + + + + E+ KH L GQLKAAESRNR+RIMRL+YQ N EI HL++CQP+A AVRLQAL+P+ +R KD L K RER E +LE+ L Sbjct: 43 YEDPAKEITEAQSVTNKRLLDRKRNEIRLNAPLPDEEYEEREKHAKLIGQLKAAESRNRLRIMRLRYQSNRSQEISHLIACQPTAARAVRLQALVPSYAERRQRKDTLDKLSRERCERLLEDSQGL 168
BLAST of Protein LKAAEAR1 vs. TrEMBL
Match: A0A2G8K9T4 (Uncharacterized protein OS=Stichopus japonicus OX=307972 GN=BSL78_18397 PE=4 SV=1) HSP 1 Score: 114.39 bits (285), Expect = 5.497e-29 Identity = 65/132 (49.24%), Postives = 88/132 (66.67%), Query Frame = 1 Query: 70 YTNPSKEVLEAQQNSKKRILDQIKA---QKKFRIVEDENSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILENDSILKRN 456 Y P K A NSKKR+ +++KA +KK DE EK K+ L GQLKAAE+RNR+RIMRL YQ N EI HL+SCQP+A++AVRLQA++P P++ + D+L K +R R+E ILE+++ L N Sbjct: 42 YEEPPKSAALAMANSKKRVQEKMKADQERKKKETSVDE----EKEKYSQLIGQLKAAEARNRLRIMRLHYQNNRAEEIRHLISCQPTAIKAVRLQAMVPPVPEKGSPADSLDKLERSRIESILEDENGLTIN 169
BLAST of Protein LKAAEAR1 vs. TrEMBL
Match: V3YYI6 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_236793 PE=4 SV=1) HSP 1 Score: 113.62 bits (283), Expect = 1.030e-28 Identity = 63/129 (48.84%), Postives = 84/129 (65.12%), Query Frame = 1 Query: 70 YTNPSKEVLEAQQNSKKRILDQIKAQKKFRIVEDENSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILENDSILKRN 456 Y PSK + E Q KKR+++ K Q+ E+ EK KH L GQLKAAE+RNR+RIMRL+YQ N E+ HL++CQPSAL+AVRLQAL+P P ++ K+ + K ERVE++LE+ L N Sbjct: 31 YEEPSKTIQETQLQCKKRLIELRKEQELKNAPPKEDELMEKEKHAKLIGQLKAAEARNRLRIMRLRYQANRAQEVSHLIACQPSALKAVRLQALVPPYPDTKSRKNKMDKLDMERVEILLEDTKGLITN 159
BLAST of Protein LKAAEAR1 vs. TrEMBL
Match: R7T5S6 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_220213 PE=4 SV=1) HSP 1 Score: 114.775 bits (286), Expect = 1.059e-28 Identity = 63/127 (49.61%), Postives = 85/127 (66.93%), Query Frame = 1 Query: 70 YTNPSKEVLEAQQNSKKRILDQIKAQKKFRIVEDENSRNE-----KLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILEN 435 Y P K + EAQ +S+KR++D K R V + +E + KH+ L GQLKAAE+RNRIRIMRL+Y+ N+ EI+HL +CQP+A +AVR+QALLPT+P R D+L K R RVE +LE+ Sbjct: 78 YEEPPKAIAEAQLSSRKRLVDL-----KNRYVRENKPPSELEIDDRDKHEKLIGQLKAAEARNRIRIMRLRYETNMGQEINHLTACQPTAGKAVRMQALLPTRPDRRPQGDSLDKISRGRVECLLED 199
BLAST of Protein LKAAEAR1 vs. Ensembl Cavefish
Match: ENSAMXT00000043437.1 (LKAAEAR motif containing 1 [Source:NCBI gene;Acc:103031616]) HSP 1 Score: 80.8777 bits (198), Expect = 1.841e-19 Identity = 58/132 (43.94%), Postives = 75/132 (56.82%), Query Frame = 1 Query: 70 YTNPSKEVLEAQQNSKKRI---LDQIKAQKKFRIVEDENSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPT---KPQRETHKDALTKSQRERVEMILENDSIL 447 Y PSKE S++R+ L Q KA++ + V D KL D L GQLKA E+RNRIR MR QY EI+ ++SCQPSA AVRL+ LL K ++ D L + QR+RVE ILE++ L Sbjct: 34 YAEPSKEAKGWMAASRQRVHARLAQQKAERNPQRVLDS-----KLHQDELIGQLKATEARNRIRQMRQQYHNLKAQEINLMISCQPSAQSAVRLELLLQAQEKKNKKTNISDGLDQLQRQRVEEILEDEKGL 160
BLAST of Protein LKAAEAR1 vs. Ensembl Nematostella
Match: EDO42816 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0D6]) HSP 1 Score: 103.219 bits (256), Expect = 2.754e-28 Identity = 58/126 (46.03%), Postives = 86/126 (68.25%), Query Frame = 1 Query: 70 YTNPSKEVLEAQQNSKKRIL---DQIKAQKKFRIVEDENSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILEND 438 Y +P+KEV E+ ++KR+ +QIK + K +E + E+ KH L GQLKAAE+RNRIR+MRL+Y+ N E++HL+SCQP+AL+AVRLQ+L+P + +D L K +R RV +LE++ Sbjct: 52 YESPAKEVAESMAAARKRLKELKNQIKKETKPPALE---TVTEQDKHVKLIGQLKAAEARNRIRLMRLRYEHNRASEVNHLISCQPTALKAVRLQSLVPPETDPIKIRDLLGKGERYRVNALLEDE 174
BLAST of Protein LKAAEAR1 vs. Planmine SMEST
Match: SMESG000010224.1 (SMESG000010224.1) HSP 1 Score: 261.151 bits (666), Expect = 9.259e-91 Identity = 129/131 (98.47%), Postives = 130/131 (99.24%), Query Frame = 1 Query: 67 IYTNPSKEVLEAQQNSKKRILDQIKAQKKFRIVEDENSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILENDSILKRNF 459 IYTNPSKEVLEAQQNSKKRILDQIKAQKKF+IVEDE SRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILENDSILKRNF Sbjct: 13 IYTNPSKEVLEAQQNSKKRILDQIKAQKKFQIVEDEKSRNEKLKHDVLFGQLKAAESRNRIRIMRLQYQKNIIGEIDHLVSCQPSALEAVRLQALLPTKPQRETHKDALTKSQRERVEMILENDSILKRNF 143 The following BLAST results are available for this feature:
BLAST of Protein LKAAEAR1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of Protein LKAAEAR1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Protein LKAAEAR1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Protein LKAAEAR1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Protein LKAAEAR1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Protein LKAAEAR1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of Protein LKAAEAR1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of Protein LKAAEAR1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Protein LKAAEAR1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Protein LKAAEAR1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Protein LKAAEAR1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Protein LKAAEAR1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Protein LKAAEAR1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Protein LKAAEAR1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020711 ID=SMED30020711|Name=Protein LKAAEAR1|organism=Schmidtea mediterranea sexual|type=transcript|length=513bpback to top protein sequence of SMED30020711-orf-1 >SMED30020711-orf-1 ID=SMED30020711-orf-1|Name=SMED30020711-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp MKEEKPKKNPNKIYTNPSKEVLEAQQNSKKRILDQIKAQKKFRIVEDENSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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