G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30020667
Length (bp)1403
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30020667) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30020667) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30020667) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30020667) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30020667

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30020667SMESG000033248.1 dd_Smed_v4_17590_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30020667SMESG000033248.1 dd_Smed_v4_17590_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: dmsr-1 (DroMyoSuppressin Receptor related [Source:UniProtKB/TrEMBL;Acc:Q8MQ19])

HSP 1 Score: 95.1301 bits (235), Expect = 7.287e-21
Identity = 97/355 (27.32%), Postives = 179/355 (50.42%), Query Frame = 3 
Query:  120 FGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQN-------NPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNI-ENVTHFFPTR-------------STKNSKLEM-YNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSIST----KLSR------RSQDHRRTTRLLVSIILLVLPTELPIACL-MLATRIKENIKC-IYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFVP 1082
            +  ++ Y ++ +C++   +N  N+++L +R M +  N IL A+A  D +V  S +      +       +P +  Y  W++  +     +L+AH++S+WL V+LA+ R + ++   +    Q   ++ +  ++ +T S+  +L  P     +I EQ + E  T   P               +  NS L    ++W   ++ K+ PC+L  +F   LL  L+  R+   +LL  ++S  +  N+ +     R SIS     KL R      R +   RTT +L++I+ ++L TELP   + +L+    E  +  IY +LGD+LD  +L  +  +F++YCSMS ++R+EF R+FVP
Sbjct:   30 YHPIHAYLSIFLCVLGTIANFCNIVVLTRRTMRTPVNMILTAMASCDTVVLFSNLIYTTHYSFVAFKFCHPKHWSYS-WALFLIAHAHLSLVAHSSSVWLSVMLALVRYVTLRSRGNMGGMQ-VTLRHSYYAVAVTVSLVAVLNAPNFLNYKINEQPLNETCTDLDPMFWNSPAYLPGIADIAKANSCLVFRLSYWISGMVFKVLPCALLSLFVWLLLRILREVRENRQRLL--KNSQHRPPNQTTTRNGQRLSISVAGNEKLGRNGSLRGRGERVDRTTHMLLAIVAVMLVTELPQGIMAVLSGMCSEEFRIYIYNNLGDILDLFSLCGSCCSFIIYCSMSGQFRNEFHRVFVP 380
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: dmsr-1 (DroMyoSuppressin Receptor related [Source:UniProtKB/TrEMBL;Acc:Q8MQ19])

HSP 1 Score: 95.1301 bits (235), Expect = 1.077e-20
Identity = 97/355 (27.32%), Postives = 179/355 (50.42%), Query Frame = 3 
Query:  120 FGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQN-------NPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNI-ENVTHFFP-------------TRSTKNSKLEM-YNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSIST----KLSR------RSQDHRRTTRLLVSIILLVLPTELPIACL-MLATRIKENIKC-IYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFVP 1082
            +  ++ Y ++ +C++   +N  N+++L +R M +  N IL A+A  D +V  S +      +       +P +  Y  W++  +     +L+AH++S+WL V+LA+ R + ++   +    Q   ++ +  ++ +T S+  +L  P     +I EQ + E  T   P               +  NS L    ++W   ++ K+ PC+L  +F   LL  L+  R+   +LL  ++S  +  N+ +     R SIS     KL R      R +   RTT +L++I+ ++L TELP   + +L+    E  +  IY +LGD+LD  +L  +  +F++YCSMS ++R+EF R+FVP
Sbjct:   30 YHPIHAYLSIFLCVLGTIANFCNIVVLTRRTMRTPVNMILTAMASCDTVVLFSNLIYTTHYSFVAFKFCHPKHWSYS-WALFLIAHAHLSLVAHSSSVWLSVMLALVRYVTLRSRGNMGGMQ-VTLRHSYYAVAVTVSLVAVLNAPNFLNYKINEQPLNETCTDLDPMFWNSPAYLPGIADIAKANSCLVFRLSYWISGMVFKVLPCALLSLFVWLLLRILREVRENRQRLL--KNSQHRPPNQTTTRNGQRLSISVAGNEKLGRNGSLRGRGERVDRTTHMLLAIVAVMLVTELPQGIMAVLSGMCSEEFRIYIYNNLGDILDLFSLCGSCCSFIIYCSMSGQFRNEFHRVFVP 380
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: dmsr-8 (DroMyoSuppressin Receptor related [Source:UniProtKB/TrEMBL;Acc:Q18475])

HSP 1 Score: 88.1965 bits (217), Expect = 1.687e-18
Identity = 97/352 (27.56%), Postives = 168/352 (47.73%), Query Frame = 3 
Query:  120 FGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIIS-------QNNPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITE------------QNIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRI--KENI-KCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLF---VPFFIIRQ 1100
            +  ++PY ++++CL   A N+  VI+L +  M S+ N++L AIA+ DILV  S +  +         + +P + + Y W+      + A ++ H  S+WL V+LA  R+  I+   S A +     Q   I  + T+ +  LL VP + T  I E            +  E+    +    + +  L    FW   +L K+ PC L + F    L ++            IRD            G  RK ++  ++++      TT +LV+++ + L  ELP   L +   I  KE     IY+HLGD++D L+LL + + F++YC+MS ++R+ F ++F   +P  IIR+
Sbjct:   27 YAHIHPYVSVILCLAGTAMNIVTVIVLTRPSMRSAVNSLLCAIALCDILVMTSVLVFVTHFLLFAGYRCDPTDYNIY-WAYFLYYHSQATVIFHATSIWLTVLLAQIRVFSIRRATSVAGESVTN-QMTCIIAVTTFIVVCLLNVPNMLTFEIIETPASLWLQCKANETAEDDMLVYLVAPSDHCGLLNIAFWTNGVLFKVVPC-LLLTFSIVALVSI------------IRD-----------VGKRRKQLAQVMNKKRMPRDHTTPMLVAVLSIFLFAELPQGVLHVFNAIFTKETFYDKIYIHLGDVMDVLSLLNSAVNFIIYCAMSRKFRAVFIQIFLTCLPQKIIRK 352
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: dmsr-7 (DroMyoSuppressin Receptor related [Source:UniProtKB/TrEMBL;Acc:O16548])

HSP 1 Score: 87.4261 bits (215), Expect = 2.143e-18
Identity = 111/402 (27.61%), Postives = 179/402 (44.53%), Query Frame = 3 
Query:   57 FSNSSQITDECSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKM-GSSTNTILLAIAISDILVCLSQ----IPLIISQNN---PNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLV--------------------PYLTTMRITEQNIENVTHFFPTRST--KNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELP-----IACLMLATRIKENIKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFVP-----FFIIRQTSDNPGSLVPLRNS 1142
            F  +S  T      L  FQ+ +  ++ Y  ++IC+  + +N  +V +L +  M  S+ N IL A+A+ DI    S     I  ++ +NN   P   H +   + +    S  L  HT SLWL V +A  R + +++  +    Q+   QR K +  L   I++++ V                    P  T  +      E V  F  + +    N +L  +N W I IL KI PC L I     L+S              IRD +EK   K +   ++  + S  L +++    RTT +LV I+L+ L TE P     I C +  T +    + +Y ++GD+LD L+L+ + + FVLYC MS+ YR  F  + +P      +  R+ S    S + L NS
Sbjct:   10 FDPNSNSTQAFLRKLAHFQRWYQPIHGYVCVLICVFGIFTNFVHVAVLSRPNMRNSAVNCILTAVAVCDIGTMASYLIYIIHFVLRRNNSCTPTFTHSWLQFLLWHVVLSITL--HTTSLWLAVAMAFIRRMTLRV--TALNSQW---QRPKFAWKLCLGIYMVVFVLCIPNMLVHEIARVEGNDWRPGKTCQKFASNYSEPVYTFMVSHAATINNCRLFKWNIWMIGILFKIIPCILLIFLSFGLVSK-------------IRD-AEKHRRKLTSVPSNASTDSKPLKKKNGTSDRTTLMLVVILLVFLITEFPQGIISILCAIFTTDVH---RYLYFYIGDVLDLLSLVNSSVNFVLYCVMSSRYRQTFWEVIIPSWACGLWTTRRGSPTELSQLQLNNS 387
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: sprr-1 (Sex Peptide Receptor (Drosophila) Related [Source:UniProtKB/TrEMBL;Acc:G5EEU4])

HSP 1 Score: 73.559 bits (179), Expect = 1.072e-13
Identity = 91/380 (23.95%), Postives = 162/380 (42.63%), Query Frame = 3 
Query:  129 LYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIP-------LIISQNNPNNLHYYFWS--VTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNIENVTHFFPTRSTKNSKL-------------------EMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLG-----IRDSSEK-------EENKNSITG--NHRKSIST-KLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCIYXXXXXXXXXXXXXXXXXXX------XXYCSMSTEYRSEFKRLFVPFFIIRQTSDNPGS 1121
            +Y Y    I  ++VA+N   V++L  + + + TN +LLA+A++++L  LS +P       L   +N+       FW   + Y+     ++  HT ++WL V LAI R ++I + PS   +++  I R+K  I    S+  ++  P L        ++      F ++  +  KL                    M+    +A+   + P  L +IF   L+  ++    RH  LL       R +  K        EN+N IT     R S+S     RR+   ++ TR+LV +ILL L TE+P A +     +  ++K  ++    L   L +    +         +YC MS ++R   +++F    +     D   S
Sbjct:   31 MYGYMMPFIVTLTVATNSFIVVVLSHKYLRTPTNYVLLAMAVTELLTGLSCLPWFTYYYTLSGYKNDLQTGLPSFWCDMIPYMAAFLPSIF-HTMAIWLTVYLAIQRYIYICV-PS-LVRKFCTIHRSKQVIFFIISVATIMYTPDLMAFHNKSHDV------FDSKRNRTMKLCYRHRAPFMLKLGDDIYYKVMFTTQTVAV--HLIPSVLLVIFTWKLVGAIRVADRRHANLLSKYSTTTRSTRRKFSELTNSSENENKITRLFKQRDSVSVGNEPRRAHGLKQNTRMLVVVILLFLITEIPAALIFTIHVLSVSLKFSFVDYQFLNILLIVRNVLIVVSYPFRFAIYCGMSQQFRDVVRQMFTGKMLTHAIRDKDNS 399
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Match: CG13229 (gene:FBgn0033579 transcript:FBtr0330602)

HSP 1 Score: 87.4261 bits (215), Expect = 2.379e-18
Identity = 101/364 (27.75%), Postives = 178/364 (48.90%), Query Frame = 3 
Query:   45 KRMNFSNSSQITDECSSH----LLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSS-TNTILLAIAISDILVCLSQIPLIISQN---NPNNLHY-YFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQ------NIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCI----YXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFV 1079
            +     N +   DEC  +    +  + + +  ++ Y +L+IC+    +N+ N+++L +++M  +  N IL  +A++D+ V L  IP    Q     P      Y W+V  L       + HT S+ L V LA++R + I+ +P+     +  +  ++ +I+L + +  +L +P     ++ E       N E + H +     K+S L  +NFW  ++L K+ PC + I+    L+  L     R  KL   RD +     K +I  N  ++ S K  R  + + RTT LLV++++L L TE P   L L + + E  KC     Y   G+L+D L L+   + FVLY  MS ++R+ F+ LF+
Sbjct:   43 QEYQMKNMTPQVDECQGYCQGEIYNWLRAYNSIHGYVSLMICIFGTIANILNIMVLTRKEMAKTPINNILKWLAVADMFVMLEYIPYTSYQYIYMGPGEKDLSYTWAVCLLVHMHFTQILHTISIGLTVTLAVWRYVAIR-HPNGGCANFL-LAHSREAILLPFILSPILCLPTYFVFQVRETYDVDKVNSEAMYHVY---FDKDSVLYRFNFWIHSVLIKLLPCGILIVISAVLMHVLCEASRRRLKL---RDYNNPA--KYAIQLNLNETKSKKPPRCDRRNDRTTLLLVAVLVLFLITEFPQGLLGLLSGVME--KCFFAHCYPPFGELMDLLALINAAVGFVLYGLMSKQFRTTFRSLFM 394
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Match: CG13229 (gene:FBgn0033579 transcript:FBtr0330603)

HSP 1 Score: 87.4261 bits (215), Expect = 2.379e-18
Identity = 101/364 (27.75%), Postives = 178/364 (48.90%), Query Frame = 3 
Query:   45 KRMNFSNSSQITDECSSH----LLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSS-TNTILLAIAISDILVCLSQIPLIISQN---NPNNLHY-YFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQ------NIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCI----YXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFV 1079
            +     N +   DEC  +    +  + + +  ++ Y +L+IC+    +N+ N+++L +++M  +  N IL  +A++D+ V L  IP    Q     P      Y W+V  L       + HT S+ L V LA++R + I+ +P+     +  +  ++ +I+L + +  +L +P     ++ E       N E + H +     K+S L  +NFW  ++L K+ PC + I+    L+  L     R  KL   RD +     K +I  N  ++ S K  R  + + RTT LLV++++L L TE P   L L + + E  KC     Y   G+L+D L L+   + FVLY  MS ++R+ F+ LF+
Sbjct:   43 QEYQMKNMTPQVDECQGYCQGEIYNWLRAYNSIHGYVSLMICIFGTIANILNIMVLTRKEMAKTPINNILKWLAVADMFVMLEYIPYTSYQYIYMGPGEKDLSYTWAVCLLVHMHFTQILHTISIGLTVTLAVWRYVAIR-HPNGGCANFL-LAHSREAILLPFILSPILCLPTYFVFQVRETYDVDKVNSEAMYHVY---FDKDSVLYRFNFWIHSVLIKLLPCGILIVISAVLMHVLCEASRRRLKL---RDYNNPA--KYAIQLNLNETKSKKPPRCDRRNDRTTLLLVAVLVLFLITEFPQGLLGLLSGVME--KCFFAHCYPPFGELMDLLALINAAVGFVLYGLMSKQFRTTFRSLFM 394
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Match: CG13229 (gene:FBgn0033579 transcript:FBtr0088206)

HSP 1 Score: 87.4261 bits (215), Expect = 2.379e-18
Identity = 101/364 (27.75%), Postives = 178/364 (48.90%), Query Frame = 3 
Query:   45 KRMNFSNSSQITDECSSH----LLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSS-TNTILLAIAISDILVCLSQIPLIISQN---NPNNLHY-YFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQ------NIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCI----YXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFV 1079
            +     N +   DEC  +    +  + + +  ++ Y +L+IC+    +N+ N+++L +++M  +  N IL  +A++D+ V L  IP    Q     P      Y W+V  L       + HT S+ L V LA++R + I+ +P+     +  +  ++ +I+L + +  +L +P     ++ E       N E + H +     K+S L  +NFW  ++L K+ PC + I+    L+  L     R  KL   RD +     K +I  N  ++ S K  R  + + RTT LLV++++L L TE P   L L + + E  KC     Y   G+L+D L L+   + FVLY  MS ++R+ F+ LF+
Sbjct:   43 QEYQMKNMTPQVDECQGYCQGEIYNWLRAYNSIHGYVSLMICIFGTIANILNIMVLTRKEMAKTPINNILKWLAVADMFVMLEYIPYTSYQYIYMGPGEKDLSYTWAVCLLVHMHFTQILHTISIGLTVTLAVWRYVAIR-HPNGGCANFL-LAHSREAILLPFILSPILCLPTYFVFQVRETYDVDKVNSEAMYHVY---FDKDSVLYRFNFWIHSVLIKLLPCGILIVISAVLMHVLCEASRRRLKL---RDYNNPA--KYAIQLNLNETKSKKPPRCDRRNDRTTLLLVAVLVLFLITEFPQGLLGLLSGVME--KCFFAHCYPPFGELMDLLALINAAVGFVLYGLMSKQFRTTFRSLFM 394
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Match: MsR2 (gene:FBgn0264002 transcript:FBtr0330360)

HSP 1 Score: 71.2478 bits (173), Expect = 7.791e-13
Identity = 46/158 (29.11%), Postives = 83/158 (52.53%), Query Frame = 3 
Query:   87 CSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIP-----LIISQNNPNNLHY-YFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYL 542
            C + +  F   +   + Y +L++C++   +N  N+I+L +R+M S TN IL  +A++D+ V L  IP      I+S   P      Y W+      +    + HT S+WL V LA++R + +  YP R  + + G++   I+I   Y + +L++ P+L
Sbjct:   11 CGTGIDDFHTNYKYFHGYFSLIVCILGTIANTLNIIVLTRREMRSPTNAILTGLAVADLAVMLEYIPYTVHDYILSVRLPREEQLSYSWACFIKFHSVFPQVLHTISIWLTVTLAVWRYIAVS-YPQR-NRIWCGMRTTLITIATAYVVCVLVVSPWL 166
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Match: MsR1 (gene:FBgn0035331 transcript:FBtr0330187)

HSP 1 Score: 70.8626 bits (172), Expect = 8.936e-13
Identity = 52/167 (31.14%), Postives = 90/167 (53.89%), Query Frame = 3 
Query:   87 CSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIP-----LIISQNNPNNLHY-YFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVP--YLTTMRITE 563
            C S +  F   +  ++ Y +LV+C++   +N  N+I+L +R+M S TN IL  +A++D+ V L  IP      I++ + P      Y W+      +  A + HT S+WL V LA++R + +  YP +  + + G++   I+I   Y + +L++ P  YL T  ITE
Sbjct:   13 CGSGMDNFHTSYKNMHGYVSLVVCILGTIANTLNIIVLTRREMRSPTNAILTGLAVADLAVMLEYIPYTIHDYILTDSLPREEKLSYSWACFIKFHSIFAQVLHTISIWLTVTLAVWRYIAVG-YPQK-NRVWCGMRTTIITITTAYVVCVLVVSPSLYLITA-ITE 176
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A1S3K1C9 (sex peptide receptor OS=Lingula unguis OX=7574 GN=LOC106178005 PE=4 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 1.851e-31
Identity = 106/343 (30.90%), Postives = 176/343 (51.31%), Query Frame = 3 
Query:   78 TDECSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPL-----IISQNNPN---NLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVP-YLTTMRITEQN-IENVTHFFPTRSTKNS---KLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIK-ENIKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEF 1064
            +D  +S L  F+  +   + + +LV+C   + SN+ N+ +L ++ M + TN IL  +A++D+L  +S +P      I+   +P        Y W    L   + A+  HTAS+++GVVLA+FR  +I+   S +    FG+++A  S++  Y++ ++ L+P YL    + E + + N T+F    S        +   NFW  AI  KI PC    +FG  LL T             IR ++ K         N  +++ ++L    ++H RTTR+LV +I+L L TE P   L L + +  E  + +Y  LGDL+D + L+ N + F+LYCSMS  +R  F
Sbjct:   29 SDHVASPLETFETWYAGYHGFVSLVVCTFGIISNMFNIAVLTRKGMRTPTNLILTGLAVADMLTMMSYVPFAFHFRILHNTSPTITPERDSYGWIAFLLFHANFAVTTHTASIFVGVVLAVFRYAYIR--ASSSGYVTFGMEKAFFSVMFAYALSVVFLIPNYLAQSIVQETDRVTNKTYFRLDGSLSEELFPNINQINFWIHAIAIKITPCIFMTVFGGLLLHT-------------IRQTTAKGREMQRFGSNRSQALKSRL----REHSRTTRMLVVVIILFLFTEFPQGVLALLSGVHTEYFESVYSPLGDLMDIVALINNSINFILYCSMSKSFRQAF 352
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A194QMF6 (Putative G-protein coupled receptor B0563.6 OS=Papilio machaon OX=76193 GN=RR48_14577 PE=4 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.657e-29
Identity = 110/348 (31.61%), Postives = 173/348 (49.71%), Query Frame = 3 
Query:   87 CSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQN---NPN-NLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNI--ENVTHFFPTRS------------TKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKEN-IKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRL 1073
            C     +F K + +L+ Y A+VIC++  A+N  N+ +L +R+M SSTN+IL  +A++D+LV L  IP  +  N    P  N + Y W+V     +  +   HT S+WL V LA++R + I  +P +  + +   Q   I+I+  Y I   L +P    M I++  +  +N + F    S            +KN +L     W  +++ K+ P     I   CL+S L  T  R  KLL        +  K  +  +         +RRS    RTTR+L++++ L L TE+P   L LA+ +  +  K  Y   GDL+D L L  + + FVLYCSMS ++R  F RL
Sbjct:    9 CVPGATEFNKAYSRLHGYIAIVICIIGSATNSINIAVLSRREMISSTNSILTGLAVADLLVMLEYIPYALHMNIKIGPEVNKNTYAWTVFVYFHSIFSQTFHTISIWLAVTLAVWRYIAIA-FPQK-NRTWCSKQNTTIAIVSAYVICPFLCLPIYFAMNISQNEVTPQNNSQFAEDLSLPHNRTVYVLEMSKNKELVTAIMWIYSVILKLVPSIALSILSTCLISKLTTTERRRQKLLKRSIVGPNDSEKQCLAED------PSAARRSSRTDRTTRMLLAVLGLFLSTEVPQGLLGLASALAPDFFKSCYSMFGDLMDVLALFTSSVNFVLYCSMSRQFRCTFARL 348
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A194PKB9 (Putative G-protein coupled receptor B0563.6 OS=Papilio xuthus OX=66420 GN=RR46_15044 PE=4 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 1.783e-29
Identity = 110/348 (31.61%), Postives = 173/348 (49.71%), Query Frame = 3 
Query:   87 CSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQN---NPN-NLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNI--ENVTHFFPTRS------------TKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKEN-IKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRL 1073
            C     +F K + +L+ Y A+VIC++  A+N  N+ +L +R+M SSTN+IL  +A++D+LV L  IP  +  N    P  N + Y W+V     +  +   HT S+WL V LA++R + I  +P +  + +   Q   I+I+  Y I   L +P    M I++  +  +N + F    S            +KN +L     W  +++ K+ P     I   CL+S L  T  R  KLL        +  K  +  +         +RRS    RTTR+L++++ L L TE+P   L LA+ +  +  K  Y   GDL+D L L  + + FVLYCSMS ++R  F RL
Sbjct:    9 CVPGATEFNKAYSRLHGYIAIVICIIGSATNSINIAVLSRREMISSTNSILTGLAVADLLVMLEYIPYALHMNIKIGPEVNKNTYAWTVFVYFHSIFSQTFHTISIWLAVTLAVWRYIAIA-FPQK-NRTWCSKQNTTIAIVSAYVICPFLCLPIYFAMNISQNEVTPQNNSQFAEDLSLPHNRTVYVLEMSKNKELVTAIMWIYSVILKLVPSIALSILSTCLISKLTTTERRRQKLLKRSIVGPNDSEKQCLAED------PSAARRSSRTDRTTRMLLAVLGLFLSTEVPQGLLGLASALAPDFFKSCYSMFGDLMDVLALFTSSVNFVLYCSMSRQFRCTFARL 348
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: Q5FB96 (Myosuppressin receptor OS=Bombyx mori OX=7091 GN=bmsr PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 2.929e-29
Identity = 114/356 (32.02%), Postives = 180/356 (50.56%), Query Frame = 3 
Query:   87 CSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPL-------IISQNNPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQR-AKISIILTYSIWILLLVPYLTTMRITEQ------NIENVT------------HFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCI-YXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRL 1073
            CS     FQ+V+ Q + Y AL+ICL+  A+N  N+ +L +++M S TN+IL  +A++D+LV +  IPL       I S+ N N+   Y W+V     +  +   HT S+WL ++LA++R + IK +P +   Q    +R   ++IIL Y++  +L +P    M I E+      N  NVT              F    T N+ L    FW  ++  K+ PC +  I    L+  +K +  R  KLL     +  E  K  +  + +K              RTTR+LV+++ L L TELP A   L T I  ++  I Y   G+++D + L+ + + FVLYCSMS ++R+ F RL
Sbjct:   10 CSESATAFQRVYIQAHGYIALIICLLGSAANSVNIAVLSRKEMTSCTNSILTGLAVADLLVMIDYIPLALHLYTKIGSELNQNS---YGWAVFIYFHSIFSQTFHTISIWLTIMLAVWRYIAIK-FPQK--NQTLCNKRNTTLAIILAYAVCPVLCLPIYFAMNIKERLPSDTSNGTNVTLNYTSAANVSDPKQFIIEMTNNNDLLTAIFWIYSVFIKLIPCVVLSILSVLLIMKMKSSDRRRQKLLKKSAITTTEGEKARLNDDGKKG--------GGRTDRTTRMLVALLGLFLATELPQALFGLLTAIAPHLFLICYYAFGEVMDLMALVGSAVNFVLYCSMSRQFRTTFTRL 351
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0K0PUS0 (Orphan G-protein coupled receptor 16 OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 4.004e-29
Identity = 105/355 (29.58%), Postives = 190/355 (53.52%), Query Frame = 3 
Query:   51 MNFSNSSQITDECSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNL------HYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNIENVTHF----FPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACL-MLATRIKENIKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFVP 1082
            M+  N++++    S  L  F   +   + Y +LV+C   + SN+ N+++L ++ M ++TN IL  +A SD+L  +S +P  +     + L      + + W + +L   + ++  HT S WLGV+L+++R  +I+L    + K    + + KI ++L Y+  +++L+P   ++ I    ++N T +      T +T    +   NFW  A++ K+ PC++  IFG  L+ T++ T  R          S++   +N++       +ST    R ++H RTTR+LV +I+L L TELP   L +L   +    + +Y+ LGD++D + L+ N + F LYCSMS ++R  F RLF P
Sbjct:    1 MDAENATRLYGTQS--LKSFSDWYASYHGYVSLVVCAYGIISNIFNILVLTRKNMWTTTNCILTGLAFSDLLTMMSYVPFALQFYVLHGLDASPQRNSHPWVLFFLFHVNFSVTTHTISTWLGVLLSVYRYTYIRLSSDSSVKC--SLAKTKIMVLLVYAYSMMVLIPNYLSVEIRSYPMDNNTIYNLAGIDTNTTYGITITTINFWLHALVIKLIPCAMMSIFGFLLVFTMRSTHAR----------SKRLRRQNTV-------VSTINKTRKKEHSRTTRMLVVVIILFLITELPHGILALLCGLLPGFFEAVYVPLGDVMDIVALINNAVNFTLYCSMSKQFRETFIRLFCP 334
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Match: npy8br (neuropeptide Y receptor Y8b [Source:ZFIN;Acc:ZDB-GENE-980526-208])

HSP 1 Score: 48.9062 bits (115), Expect = 1.291e-6
Identity = 58/241 (24.07%), Postives = 108/241 (44.81%), Query Frame = 3 
Query:  147 LVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFG----IQRAKISIILTYSIWILLLV---PYLTTMRITEQNIENVTHFFPTRSTKNSKLEMYN------FWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKL 830
            ++I L  + ++L  ++IL   ++ + TN +++ +A SD+LV L  +PL I+    +  H+ F     LC  S  L     S+      +IF L+ I +   +      G    +  A ++I    +IW+       P+L    +T++   N++H+FP    K + +E++        +  ++L   F C L  +F C L   L+  R R   L      + +E   N +  +H K I+  L
Sbjct:   47 VLIALGLLGNSLLVLVILRHSELHNVTNILIVNLAFSDMLVGLVCLPLTIAYTLMD--HWIFGEA--LCKGSPFLQCSAVSV------SIFSLVLIAIERHQLIINPTGWKPSLNHAYVAI---GAIWVTAFAMSSPFLAFHVLTDEPYRNLSHYFPDYGEKVACIELWPSDTSKFAFTTSLLVFQFSCPLLFVFLCYLRIFLRL-RQRKKML-----PTGREGGGNGVRASHMKKINMML 268
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000033248.1 (SMESG000033248.1)

HSP 1 Score: 810.831 bits (2093), Expect = 0.000e+0
Identity = 431/432 (99.77%), Postives = 431/432 (99.77%), Query Frame = 3 
Query:   51 MNFSNSSQITDECSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFVPFFIIRQTSDNPGSLVPLRNSEVEAEHKRSFINISNTADFPKYCLISDSHFPTTSFRKDFKTYQSVEPLNKFLYVHDIERSISTYCNDQ 1346
            MNFSNSSQITDECSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCIYLHLGDLLDFLNLLKNFLTFVLYCSMSTEYRSEFKRLFVPFFIIRQTSDNPGSLVPLRNSEVEAEHKRSFINISNTADFPKYCLISDSHFPTTSFRKDFKTYQSVEPLNKFLYV DIERSISTYCNDQ
Sbjct:    1 MNFSNSSQITDECSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCIYLHLGDLLDFLNLLKNFLTFVLYCSMSTEYRSEFKRLFVPFFIIRQTSDNPGSLVPLRNSEVEAEHKRSFINISNTADFPKYCLISDSHFPTTSFRKDFKTYQSVEPLNKFLYVQDIERSISTYCNDQ 432
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000009975.1 (SMESG000009975.1)

HSP 1 Score: 138.272 bits (347), Expect = 4.459e-36
Identity = 113/371 (30.46%), Postives = 198/371 (53.37%), Query Frame = 3 
Query:   51 MNFSNSSQITDECS-SHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIIS---------------QNNPNNLHYYFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIW---ILLLVPYLTTMRITEQ-----NIENVT----HFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFV 1079
            MNF+N++   ++C   +L KF K F  LY Y   ++C+VS+ +N+ NV +L  ++M + TN ILL++A  DI+ CL Q+P++I                 NN      + W   Y+ +++ +L++H  ++WLG  LA+FR   ++     +A +Y   Q  + + ++ ++     I L++PYL T  I  +     NI N T     +  +R      L+ Y++W ++IL KI P  +  +F   LL ++                ++ EE K S+    R S +    R + +H R TRLL+ I++++L  E+P A +   +RI +   C+Y +LGDL+D L+L KN +TF+LYC MS+++R+ F + ++
Sbjct:    1 MNFNNTTSTLNQCDFRNLRKFHKRFLPLYGYLGALVCVVSIFTNILNVFVLNHKRMKTPTNLILLSLAALDIVTCLVQLPVLIKFYILDIEILMALRPWYNN------WGWVFYYIFSSNVSLISHNTTVWLGSALAVFRYHIVRQCTVSSALRYRATQEKRRAFVIVFTCLVFNIFLIIPYLFTQTIEYKLKNVANISNQTDRYFQYQASRYLNKPILDFYSYWMVSILDKIIPSIVMAVFIGALLHSI----------------AQNEERKRSLVF-ERLSTNNLTRRGNNNHHRATRLLIVIVIIILIAEVPHAIMQFLSRIHKKSYCVYTYLGDLMDLLSLCKNSITFILYCLMSSDFRTIFMKFYM 348
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000046994.1 (SMESG000046994.1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.224e-20
Identity = 102/349 (29.23%), Postives = 165/349 (47.28%), Query Frame = 3 
Query:  102 LKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHYY----------------FWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQN------IENVTHFFPTRSTKNS--KLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKCIYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLF 1076
            L+F  +FG    Y + ++C++ + +NL+N+I+L Q  M S TN +L  +AI+D+L  +  +        PN  HYY                 W+   L   S  L++H A++W  VVLA+ R L + L P+    Q   I+   ISI +   + ++  +P    M I E N       +  +  F T     S  KLE+ NFW  +I+ KI P  +  +    L+  ++    R +KL+  R  S+K++ +N                      R T +L+ +++L L  ELP A LML++   +    IY  LGDL+DFL L+   + F+LY +MS ++R  F   F
Sbjct:   21 LRFYTIFG----YGSALMCIIGILTNLANIIVLSQPTMRSPTNWVLTWLAIADLLTMVVYL--------PNVCHYYIFNNIILPLFSVTSNKIWAYYRLTIISFVLISHAAAVWFTVVLAVIRYLHLSL-PT-IGPQIANIENTTISIAIVSVLSLIFTIP---NMMINEVNRCVGFTFDKQSKAFHTFYEIKSINKLEI-NFWIQSIIFKIVPSFVLSVLTILLILKMQHIGKRRNKLIS-RSDSQKKKKEN----------------------RVTLMLLMVVVLFLIVELPHAILMLSSNFSQTCLEIYETLGDLIDFLTLVNESINFILYTTMSKQFRRVFIMTF 328
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000056731.1 (SMESG000056731.1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.237e-18
Identity = 98/348 (28.16%), Postives = 169/348 (48.56%), Query Frame = 3 
Query:   96 HLLKFQKVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHYYFWSVTY--LCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNIENVTHFFPTRST-----KNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLG--IRDSSEKE---ENKNSITGNHRKSISTKLSRR-----SQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKENIKC----IYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLF 1076
            +L  F   +  ++ +  L+IC + +  N+ N+IIL Q+ M S TN +L  +A+SD  +    I   +     NNL    WS  +  L   S     H+ S W  V LA+FRL++I+ YP  A   +  + RAKIS +    + IL+ VPY+    +      N++ F  +  T      N +++   F+  +I  K  P +   I    L+S L+ +  R   LL   +  +S +E    N  S     R  I+     +     ++   +TTR+L++I+++   + LP A ++L + +    +C    +Y  +G+L+D + LL   + F+L+CSMS++YR  F  LF
Sbjct:   15 NLKHFYDAYDAVHVHLVLLICPLGLVFNILNIIILNQKSMKSPTNFLLTLLALSDSTIMALYIIFDLKFLKTNNLDTMTWSWAHYLLFYLSMQNFLHSFSSWNLVALAVFRLIYIR-YPHNAVV-HCTMNRAKISGLTIVGVSILISVPYILAHNVMPVATSNLSIFNDSNKTFMINYTNPQMQKVIFFWTSIFVKSLPITWMTILTVILISALRQSVMRRQTLLNSMLVANSPQEVLISNARSNILQRRGGITKDTIGKVDPVGAKKSIKTTRMLIAIVIMYCFSYLPQALMLLTSGVLG--QCFSVHVYDKMGNLMDLITLLNACVNFILFCSMSSQYRQTFTELF 358
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000016449.1 (SMESG000016449.1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.724e-17
Identity = 96/331 (29.00%), Postives = 160/331 (48.34%), Query Frame = 3 
Query:  114 KVFGQLYPYAALVICLVSVASNLSNVIILGQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHY--YFWSVTYLCTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISIILTYSIWILLLVPYLTTMRITEQNIENVTHFFPTRSTKNSKLEMYNFWNIAILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITGNHRKSISTKLSRRSQDHRR---TTRLLVSIILLVLPTELPIACLMLATRIKENIKC----IYXXXXXXXXXXXXXXXXXXXXXYCSMSTEYRSEFKRLFV 1079
            K +  L+ +  L+IC + + +NL   I L Q  M S TN +L  +AISD L+    I   +      +L      W+   L +     + H  S +L V LA+FRL++I+ YP + A  +  ++RA+ ++++ +   I+L +PY+T  ++T  N     + F          +  + W+ +I  K  P  L       L+  LK +  R  KL        K ++ N  + +  K ++ K    ++   R   TTR+L++II + L +  P A L+L   I    +C    +Y  LGD++D L LL   + FVLYCSMS ++R  F  LF+
Sbjct:   26 KFYNSLHIHLVLIICPLGLITNLLTAITLNQPAMKSPTNLLLTFLAISDGLIMALYIVFDLKFLKSGSLQKSPLIWAQYLLFSLVVQNILHLLSSYLIVSLAVFRLIYIR-YP-QLAIVHCTMKRARYTVVIIFVFAIILSIPYITAHKVTPVNNSTNNNAFTVDYVSVELGKFVHVWS-SIFAKTLPIILMTGMSLWLIYELKGSEKRRQKL--------KSDHSNIQSDSITKPLNCKTKHVTESSNRTIKTTRILLAIIFIFLLSYFPQATLLLLAGIFG--ECFSSNVYDKLGDVMDLLTLLNACIYFVLYCSMSKQFRDTFFVLFI 343
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dmsr-17.287e-2127.32DroMyoSuppressin Receptor related [Source:UniProt... [more]
dmsr-11.077e-2027.32DroMyoSuppressin Receptor related [Source:UniProt... [more]
dmsr-81.687e-1827.56DroMyoSuppressin Receptor related [Source:UniProt... [more]
dmsr-72.143e-1827.61DroMyoSuppressin Receptor related [Source:UniProt... [more]
sprr-11.072e-1323.95Sex Peptide Receptor (Drosophila) Related [Source... [more]
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG132292.379e-1827.75gene:FBgn0033579 transcript:FBtr0330602[more]
CG132292.379e-1827.75gene:FBgn0033579 transcript:FBtr0330603[more]
CG132292.379e-1827.75gene:FBgn0033579 transcript:FBtr0088206[more]
MsR27.791e-1329.11gene:FBgn0264002 transcript:FBtr0330360[more]
MsR18.936e-1331.14gene:FBgn0035331 transcript:FBtr0330187[more]
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3K1C91.851e-3130.90sex peptide receptor OS=Lingula unguis OX=7574 GN=... [more]
A0A194QMF61.657e-2931.61Putative G-protein coupled receptor B0563.6 OS=Pap... [more]
A0A194PKB91.783e-2931.61Putative G-protein coupled receptor B0563.6 OS=Pap... [more]
Q5FB962.929e-2932.02Myosuppressin receptor OS=Bombyx mori OX=7091 GN=b... [more]
A0A0K0PUS04.004e-2929.58Orphan G-protein coupled receptor 16 OS=Platynerei... [more]
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
npy8br1.291e-624.07neuropeptide Y receptor Y8b [Source:ZFIN;Acc:ZDB-G... [more]
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000033248.10.000e+099.77SMESG000033248.1[more]
SMESG000009975.14.459e-3630.46SMESG000009975.1[more]
SMESG000046994.13.224e-2029.23SMESG000046994.1[more]
SMESG000056731.12.237e-1828.16SMESG000056731.1[more]
SMESG000016449.11.724e-1729.00SMESG000016449.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020667 ID=SMED30020667|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1403bp
CTGAACTCATAATAAACAGAATGGATTCAAACAATTATTTTTGAAAAAGA
ATGAATTTTTCCAACTCCTCTCAAATAACTGATGAATGCTCTTCTCATCT
GTTGAAATTCCAGAAAGTATTTGGTCAGTTGTATCCCTACGCAGCTTTGG
TTATTTGCTTGGTGTCAGTGGCCTCGAATCTCAGTAATGTTATCATTTTA
GGGCAAAGAAAAATGGGGTCGTCTACAAACACAATTCTCTTGGCAATCGC
CATAAGCGATATTCTCGTTTGCCTTTCCCAAATTCCCCTAATAATCAGTC
AGAACAATCCCAATAATCTCCATTATTACTTTTGGTCTGTTACATATCTG
TGCACTAATAGTGCAGCTTTATTGGCGCATACAGCGTCTCTTTGGTTGGG
TGTTGTTTTGGCAATTTTTCGTTTACTTTTTATAAAGCTATATCCATCTC
GAGCGGCCAAACAATACTTTGGAATCCAGAGAGCTAAAATTTCAATTATT
TTGACATACTCAATCTGGATCTTACTTCTTGTGCCGTATCTGACTACAAT
GCGTATTACTGAACAAAATATAGAAAATGTCACTCATTTCTTTCCAACAA
GAAGCACCAAAAATTCCAAACTGGAAATGTATAACTTTTGGAATATTGCG
ATTCTTGGAAAGATTTTTCCTTGCTCCCTGACGATAATTTTTGGTTGTTG
TTTATTGTCAACTTTAAAATTTACCCGAGATCGTCATGACAAATTACTCG
GAATTCGAGATTCATCGGAGAAAGAGGAGAATAAGAATTCTATCACAGGA
AATCACAGGAAAAGCATTTCGACAAAACTCTCTCGTAGAAGTCAAGATCA
TCGTAGGACAACGAGGTTGTTGGTTAGCATTATTCTTTTGGTACTACCAA
CTGAGTTACCCATAGCCTGTTTAATGTTGGCTACAAGAATCAAAGAAAAT
ATTAAATGTATTTATCTTCATCTTGGAGATCTTTTAGACTTTTTAAATCT
GTTAAAAAATTTCCTGACTTTTGTGCTATATTGTTCCATGTCTACTGAAT
ATCGAAGTGAATTTAAAAGACTTTTTGTTCCATTTTTCATCATTCGACAG
ACATCTGACAATCCAGGATCATTGGTTCCTTTGAGAAACAGCGAAGTCGA
GGCAGAACATAAAAGGTCGTTTATCAATATATCTAACACGGCAGACTTTC
CTAAATACTGTTTAATCTCAGATAGCCATTTTCCAACGACAAGTTTTCGT
AAAGACTTCAAAACTTATCAATCAGTTGAACCTTTGAATAAATTTCTTTA
TGTTCATGACATAGAAAGGTCGATTTCAACATATTGCAATGACCAATGAT
TACATGAGAGATTTATAATTTTCTATAACTCATAATTTTTCAGATTAATT
TTA
back to top

protein sequence of SMED30020667-orf-1

>SMED30020667-orf-1 ID=SMED30020667-orf-1|Name=SMED30020667-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=433bp
MNFSNSSQITDECSSHLLKFQKVFGQLYPYAALVICLVSVASNLSNVIIL
GQRKMGSSTNTILLAIAISDILVCLSQIPLIISQNNPNNLHYYFWSVTYL
CTNSAALLAHTASLWLGVVLAIFRLLFIKLYPSRAAKQYFGIQRAKISII
LTYSIWILLLVPYLTTMRITEQNIENVTHFFPTRSTKNSKLEMYNFWNIA
ILGKIFPCSLTIIFGCCLLSTLKFTRDRHDKLLGIRDSSEKEENKNSITG
NHRKSISTKLSRRSQDHRRTTRLLVSIILLVLPTELPIACLMLATRIKEN
IKCIYLHLGDLLDFLNLLKNFLTFVLYCSMSTEYRSEFKRLFVPFFIIRQ
TSDNPGSLVPLRNSEVEAEHKRSFINISNTADFPKYCLISDSHFPTTSFR
KDFKTYQSVEPLNKFLYVHDIERSISTYCNDQ*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR0194277TM_GPCR_serpentine_rcpt_Srw
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
GO:0008528G-protein coupled peptide receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 27..51
score: 17.9
coord: 106..128
score: 23.76
coord: 309..335
score: 16.74
coord: 59..80
score: 29.23
IPR0194277TM GPCR, serpentine receptor class w (Srw)PFAMPF103247TM_GPCR_Srwcoord: 32..342
e-value: 1.1E-24
score: 87.3
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 19..346
e-value: 1.0E-38
score: 135.1
NoneNo IPR availablePANTHERPTHR46273FAMILY NOT NAMEDcoord: 6..353
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 27..338
e-value: 5.63868E-34
score: 126.979
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 3..356
NoneNo IPR availableTMHMMTMhelixcoord: 147..169
NoneNo IPR availableTMHMMTMhelixcoord: 27..49
NoneNo IPR availableTMHMMTMhelixcoord: 273..295
NoneNo IPR availableTMHMMTMhelixcoord: 104..126
NoneNo IPR availableTMHMMTMhelixcoord: 62..84
NoneNo IPR availableTMHMMTMhelixcoord: 197..219
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 41..327
score: 17.042