E3 ubiquitin-ligase RNF185-like

Overview


Name	E3 ubiquitin-ligase RNF185-like
Smed ID	SMED30020665
Length (bp)	1253

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of E3 ubiquitin-ligase RNF185-like (SMED30020665) t-SNE clustered cells

Violin plots show distribution of expression levels for E3 ubiquitin-ligase RNF185-like (SMED30020665) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of E3 ubiquitin-ligase RNF185-like (SMED30020665) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for E3 ubiquitin-ligase RNF185-like (SMED30020665) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30020665

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
posterior side	SMED30020665	h1SMcG0002844	dd_Smed_v4_5004_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
posterior muscle cell	SMED30020665	h1SMcG0002844	dd_Smed_v4_5004_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Human
Match: RNF185 (ring finger protein 185 [Source:HGNC Symbol;Acc:HGNC:26783])

HSP 1 Score: 175.252 bits (443), Expect = 2.432e-52
Identity = 93/176 (52.84%), Postives = 112/176 (63.64%), Query Frame = 1

Query:  160 ETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            E+ G +S+  F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   29 ESGGQDST--FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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Query:  160 ETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            E+ G +S+  F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   29 ESGGQDST--FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Human
Match: RNF5 (ring finger protein 5 [Source:HGNC Symbol;Acc:HGNC:10068])

HSP 1 Score: 151.369 bits (381), Expect = 2.147e-43
Identity = 71/142 (50.00%), Postives = 91/142 (64.08%), Query Frame = 1

Query:  142 GSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDT-GVNMSFGIGAFPLGLFATTFN 564
            G   GP      +  +F+CNICLE +++ V+S+CGHL+CWPCLH+W+ET+  R  CPVCKA ISR+KV+PLYGR S   +DPR K PPRPQGQR   +           +    +P  DT G + SFG+GAFP G F T FN
Sbjct:    9 GGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPE-----------SRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFN 139

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Query:  142 GSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDT-GVNMSFGIGAFPLGLFATTFN 564
            G   GP      +  +F+CNICLE +++ V+S+CGHL+CWPCLH+W+ET+  R  CPVCKA ISR+KV+PLYGR S   +DPR K PPRPQGQR   +           +    +P  DT G + SFG+GAFP G F T FN
Sbjct:    9 GGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPE-----------SRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFN 139

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Query:  142 GSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDT-GVNMSFGIGAFPLGLFATTFN 564
            G   GP      +  +F+CNICLE +++ V+S+CGHL+CWPCLH+W+ET+  R  CPVCKA ISR+KV+PLYGR S   +DPR K PPRPQGQR   +           +    +P  DT G + SFG+GAFP G F T FN
Sbjct:    9 GGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPE-----------SRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFN 139

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Celegans
Match: rnf-5 (E3 ubiquitin ligase rnf-5 [Source:UniProtKB/Swiss-Prot;Acc:Q09463])

HSP 1 Score: 127.872 bits (320), Expect = 1.519e-34
Identity = 64/133 (48.12%), Postives = 89/133 (66.92%), Query Frame = 1

Query:  163 TAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTF 561
            ++  + S  F+CNICL+ +KD V+S+CGHLFCWPCL +W++T+ +   CPVCK+AI  +KV+P+YGR   D  DPR K+PPRP+GQR+E     F+G     +G  +  G    V+ SFGIG FPL   A+ F
Sbjct:   15 SSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQVCPVCKSAIDGNKVVPIYGRGG-DSSDPRKKVPPRPKGQRSEPPPQSFAGFNWGGDGGMMG-GGGPNVHFSFGIGIFPLSFVASFF 145

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Celegans
Match: rnf-5 (E3 ubiquitin ligase rnf-5 [Source:UniProtKB/Swiss-Prot;Acc:Q09463])

HSP 1 Score: 120.939 bits (302), Expect = 9.287e-32
Identity = 49/89 (55.06%), Postives = 69/89 (77.53%), Query Frame = 1

Query:  163 TAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTE 429
            ++  + S  F+CNICL+ +KD V+S+CGHLFCWPCL +W++T+ +   CPVCK+AI  +KV+P+YGR   D  DPR K+PPRP+GQR+E
Sbjct:   15 SSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQVCPVCKSAIDGNKVVPIYGRGG-DSSDPRKKVPPRPKGQRSE 102

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Celegans
Match: C32D5.11 (pep chromosome:WBcel235:II:6352542:6355911:-1 gene:WBGene00016318.1 transcript:C32D5.11.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C32D5.11)

HSP 1 Score: 48.9062 bits (115), Expect = 6.316e-6
Identity = 25/66 (37.88%), Postives = 35/66 (53.03%), Query Frame = 1

Query:  154 GPETAGNNSSGSFDCNICL--EISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKV 345
             P T+ NN      C ICL    S++  I  C H FC+ C+  W+  QS R +CP+C+  +  DKV
Sbjct:   54 SPSTSSNNPVEELQCTICLSTRFSQECRIEGCNHSFCFSCISEWV-CQSLRPSCPMCRKDV--DKV 116

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Fly
Match: CG8974 (gene:FBgn0030693 transcript:FBtr0074188)

HSP 1 Score: 166.392 bits (420), Expect = 2.637e-48
Identity = 90/195 (46.15%), Postives = 114/195 (58.46%), Query Frame = 1

Query:  130 GFPSGSGTGPETAGNNSSGS---------FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            G+P G GT  +T  N              ++CNICL+ +KD V+SMCGHLFCWPCLH+W+ T+ +R  CPVCKAA+ +DKVIPLYGR+ST Q DPR+K+PPRP GQRTE D                  G   G +MSFGIGAFP G   +T NF     P  +   R T  Y  +Q+  LSK+F  +A    +W
Sbjct:   95 GYPFG-GTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPD----------PVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAAN---RGTRQYEDEQT--LSKLFSYLAVVWILW 273

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Fly
Match: CG8974 (gene:FBgn0030693 transcript:FBtr0074186)

HSP 1 Score: 166.392 bits (420), Expect = 2.637e-48
Identity = 90/195 (46.15%), Postives = 114/195 (58.46%), Query Frame = 1

Query:  130 GFPSGSGTGPETAGNNSSGS---------FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            G+P G GT  +T  N              ++CNICL+ +KD V+SMCGHLFCWPCLH+W+ T+ +R  CPVCKAA+ +DKVIPLYGR+ST Q DPR+K+PPRP GQRTE D                  G   G +MSFGIGAFP G   +T NF     P  +   R T  Y  +Q+  LSK+F  +A    +W
Sbjct:   95 GYPFG-GTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPD----------PVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAAN---RGTRQYEDEQT--LSKLFSYLAVVWILW 273

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Fly
Match: CG8974 (gene:FBgn0030693 transcript:FBtr0074187)

HSP 1 Score: 166.392 bits (420), Expect = 2.637e-48
Identity = 90/195 (46.15%), Postives = 114/195 (58.46%), Query Frame = 1

Query:  130 GFPSGSGTGPETAGNNSSGS---------FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            G+P G GT  +T  N              ++CNICL+ +KD V+SMCGHLFCWPCLH+W+ T+ +R  CPVCKAA+ +DKVIPLYGR+ST Q DPR+K+PPRP GQRTE D                  G   G +MSFGIGAFP G   +T NF     P  +   R T  Y  +Q+  LSK+F  +A    +W
Sbjct:   95 GYPFG-GTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPD----------PVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAAN---RGTRQYEDEQT--LSKLFSYLAVVWILW 273

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Fly
Match: CG8974 (gene:FBgn0030693 transcript:FBtr0074185)

HSP 1 Score: 166.392 bits (420), Expect = 2.637e-48
Identity = 90/195 (46.15%), Postives = 114/195 (58.46%), Query Frame = 1

Query:  130 GFPSGSGTGPETAGNNSSGS---------FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            G+P G GT  +T  N              ++CNICL+ +KD V+SMCGHLFCWPCLH+W+ T+ +R  CPVCKAA+ +DKVIPLYGR+ST Q DPR+K+PPRP GQRTE D                  G   G +MSFGIGAFP G   +T NF     P  +   R T  Y  +Q+  LSK+F  +A    +W
Sbjct:   95 GYPFG-GTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPD----------PVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAAN---RGTRQYEDEQT--LSKLFSYLAVVWILW 273

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Fly
Match: CG8974 (gene:FBgn0030693 transcript:FBtr0074189)

HSP 1 Score: 166.392 bits (420), Expect = 2.637e-48
Identity = 90/195 (46.15%), Postives = 114/195 (58.46%), Query Frame = 1

Query:  130 GFPSGSGTGPETAGNNSSGS---------FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            G+P G GT  +T  N              ++CNICL+ +KD V+SMCGHLFCWPCLH+W+ T+ +R  CPVCKAA+ +DKVIPLYGR+ST Q DPR+K+PPRP GQRTE D                  G   G +MSFGIGAFP G   +T NF     P  +   R T  Y  +Q+  LSK+F  +A    +W
Sbjct:   95 GYPFG-GTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPD----------PVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAAN---RGTRQYEDEQT--LSKLFSYLAVVWILW 273

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Zebrafish
Match: rnf185 (ring finger protein 185 [Source:NCBI gene;Acc:406310])

HSP 1 Score: 172.17 bits (435), Expect = 2.419e-51
Identity = 90/167 (53.89%), Postives = 108/167 (64.67%), Query Frame = 1

Query:  187 SFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            +F+CNICL+ SKD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  ++ +Q   LS++FL +A     W
Sbjct:   38 TFECNICLDTSKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAAPG---TPQHTDEQ--FLSRLFLFVALLIMFW 190

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Zebrafish
Match: RNF5 (ring finger protein 5 [Source:NCBI gene;Acc:100537822])

HSP 1 Score: 130.954 bits (328), Expect = 9.335e-36
Identity = 74/150 (49.33%), Postives = 86/150 (57.33%), Query Frame = 1

Query:  172 NNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAY 621
            +    +F+CNICL+ ++D VIS+CGHLFC             R  CPVCKA ISRDKVIPLYGR S+ Q DPR K PPRPQGQR+E +  G                 DTG +MSFGIGAFP G F T FN    NNP     F   DA+
Sbjct:   39 DRERATFECNICLDTARDAVISLCGHLFC-------------RQQCPVCKAGISRDKVIPLYGRGSSSQEDPRLKTPPRPQGQRSEPESRGPFQGFG-----------DTGFHMSFGIGAFPFGFFTTVFN---TNNP-----FHRADAH 156

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Zebrafish
Match: hltf (helicase-like transcription factor [Source:ZFIN;Acc:ZDB-GENE-030131-3306])

HSP 1 Score: 55.4546 bits (132), Expect = 7.968e-8
Identity = 23/70 (32.86%), Postives = 39/70 (55.71%), Query Frame = 1

Query:  175 NSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRD 384
            NS    +C ICL+  +  VI+ C H+FC PC+   I ++  +A CP+C+A I   +++   G  +  + D
Sbjct:  656 NSGSDEECAICLDSLRQPVITYCAHVFCRPCICEVIRSEKEQAKCPLCRAQIKTKELVEYPGEQAETRSD 725

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Zebrafish
Match: hltf (helicase-like transcription factor [Source:ZFIN;Acc:ZDB-GENE-030131-3306])

HSP 1 Score: 55.4546 bits (132), Expect = 8.191e-8
Identity = 23/70 (32.86%), Postives = 39/70 (55.71%), Query Frame = 1

Query:  175 NSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRD 384
            NS    +C ICL+  +  VI+ C H+FC PC+   I ++  +A CP+C+A I   +++   G  +  + D
Sbjct:  690 NSGSDEECAICLDSLRQPVITYCAHVFCRPCICEVIRSEKEQAKCPLCRAQIKTKELVEYPGEQAETRSD 759

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Zebrafish
Match: rnf185 (ring finger protein 185 [Source:NCBI gene;Acc:406310])

HSP 1 Score: 45.4394 bits (106), Expect = 2.848e-6
Identity = 18/24 (75.00%), Postives = 22/24 (91.67%), Query Frame = 1

Query:  187 SFDCNICLEISKDTVISMCGHLFC 258
            +F+CNICL+ SKD VIS+CGHLFC
Sbjct:   38 TFECNICLDTSKDAVISLCGHLFC 61

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Xenopus
Match: supt16h (SPT16 homolog, facilitates chromatin remodeling subunit [Source:Xenbase;Acc:XB-GENE-979305])

HSP 1 Score: 162.155 bits (409), Expect = 1.097e-47
Identity = 81/168 (48.21%), Postives = 110/168 (65.48%), Query Frame = 1

Query:  190 FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPG-VDTGVNMSFGIGAFPLGLFATTFNFTGGNN-PQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            ++CNICLE +++ V+S+CGHL+CWPCLH+W+ET+  R  CPVCKA ISR+KVIP+YGR  ++Q+DPR K PPRPQGQR E +     G+          PG  DTG +MSFGIGAFP G F T FN    ++ P+ D    ++  + +  S     +FL+IA   F+W
Sbjct:   12 YECNICLETAREPVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISREKVIPIYGRGDSNQKDPRLKTPPRPQGQRPEPENRAGGGV----------PGFTDTGFHMSFGIGAFPFGFFTTVFNTNDLHSAPRADTGLPQSRFFGLPDS-----LFLIIAAFFFLW 164

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Xenopus
Match: RNF185 (ring finger protein 185 [Source:HGNC Symbol;Acc:HGNC:26783])

HSP 1 Score: 149.443 bits (376), Expect = 1.505e-42
Identity = 89/193 (46.11%), Postives = 114/193 (59.07%), Query Frame = 1

Query:  109 STNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            S   S+ G  SGS  G E +  +S+  F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISR+KVIPLYGR ST Q DPR+K PPRPQGQR E +  G  GI            +D   + SF + A  +  F+  F+   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   11 SAENSSPGGASGSTNG-EASSQDST--FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISREKVIPLYGRGSTGQEDPREKTPPRPQGQRPEPENRGSQGIKF----------IDGDFSSSFSV-ANCIFTFSICFDVNDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 184

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Xenopus
Match: HLTF (helicase-like transcription factor [Source:NCBI gene;Acc:394525])

HSP 1 Score: 53.1434 bits (126), Expect = 6.611e-7
Identity = 22/58 (37.93%), Postives = 37/58 (63.79%), Query Frame = 1

Query:  178 SSGS-FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVI 348
            SSGS  +C ICL+     VI+ C H+FC PC+ + I+ +   A CP+C+ ++  D+++
Sbjct:  728 SSGSDEECAICLDSLNMPVITYCAHVFCKPCICQVIQHEKQEAKCPLCRGSLRLDQLV 785

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Xenopus
Match: HLTF (helicase-like transcription factor [Source:NCBI gene;Acc:394525])

HSP 1 Score: 53.1434 bits (126), Expect = 7.038e-7
Identity = 22/58 (37.93%), Postives = 37/58 (63.79%), Query Frame = 1

Query:  178 SSGS-FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVI 348
            SSGS  +C ICL+     VI+ C H+FC PC+ + I+ +   A CP+C+ ++  D+++
Sbjct:  735 SSGSDEECAICLDSLNMPVITYCAHVFCKPCICQVIQHEKQEAKCPLCRGSLRLDQLV 792

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Xenopus
Match: chrng (cholinergic receptor, nicotinic gamma [Source:Xenbase;Acc:XB-GENE-990010])

HSP 1 Score: 50.8322 bits (120), Expect = 1.806e-6
Identity = 20/54 (37.04%), Postives = 31/54 (57.41%), Query Frame = 1

Query:  193 DCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPL 354
             C +CLE+ +    + CGHLFCW C+  W  T++    CP+C+   S  K++ L
Sbjct:  270 KCTLCLEVRRHCTATPCGHLFCWECITEWCNTKTE---CPLCREKFSPQKLVYL 320

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Mouse
Match: Rnf185 (ring finger protein 185 [Source:MGI Symbol;Acc:MGI:1922078])

HSP 1 Score: 187.193 bits (474), Expect = 3.518e-57
Identity = 102/195 (52.31%), Postives = 122/195 (62.56%), Query Frame = 1

Query:  103 AESTNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            + ST  SN+G PSGS  G   +G   S +F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:    9 SASTENSNAGGPSGSSNGTGESGGQDS-TFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Mouse
Match: Rnf185 (ring finger protein 185 [Source:MGI Symbol;Acc:MGI:1922078])

HSP 1 Score: 185.267 bits (469), Expect = 6.289e-56
Identity = 102/195 (52.31%), Postives = 122/195 (62.56%), Query Frame = 1

Query:  103 AESTNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            + ST  SN+G PSGS  G   +G   S +F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   45 SASTENSNAGGPSGSSNGTGESGGQDS-TFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 224

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Mouse
Match: Rnf5 (ring finger protein 5 [Source:MGI Symbol;Acc:MGI:1860076])

HSP 1 Score: 150.984 bits (380), Expect = 1.821e-43
Identity = 71/141 (50.35%), Postives = 89/141 (63.12%), Query Frame = 1

Query:  142 GSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFN 564
            G   GP      +S +F+CNICLE +++ V+S+CGHL+CWPCLH+W+ET+  R  CPVCKA ISR+KV+PLYGR S   +DPR K PPRPQGQR   +  G               G   G + SFG+GAFP G F T FN
Sbjct:    9 GGPEGPNRERGGASATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPF----------GDAGGFHFSFGVGAFPFGFFTTVFN 139

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Mouse
Match: Brca1 (breast cancer 1, early onset [Source:MGI Symbol;Acc:MGI:104537])

HSP 1 Score: 45.8246 bits (107), Expect = 3.319e-6
Identity = 17/49 (34.69%), Postives = 30/49 (61.22%), Query Frame = 1

Query:  190 FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISR 336
             +C ICLE+ K+ V + C H+FC  C+ + +  +   + CP+CK  I++
Sbjct:   22 LECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITK 70

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Mouse
Match: Hltf (helicase-like transcription factor [Source:MGI Symbol;Acc:MGI:1196437])

HSP 1 Score: 50.447 bits (119), Expect = 3.518e-6
Identity = 23/58 (39.66%), Postives = 35/58 (60.34%), Query Frame = 1

Query:  178 SSGS-FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVI 348
            SSGS  +C ICL+     VI+ C H+FC PC+ + I ++   A CP+C+  I  D ++
Sbjct:  747 SSGSDEECAICLDSLTFPVITHCAHVFCKPCICQVIHSEQPHAKCPLCRNEIHGDNLL 804

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BLAST of E3 ubiquitin-ligase RNF185-like vs. UniProt/SwissProt
Match: sp|Q91YT2|RN185_MOUSE (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 2.461e-56
Identity = 102/195 (52.31%), Postives = 122/195 (62.56%), Query Frame = 1

Query:  103 AESTNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            + ST  SN+G PSGS  G   +G   S +F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:    9 SASTENSNAGGPSGSSNGTGESGGQDS-TFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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BLAST of E3 ubiquitin-ligase RNF185-like vs. UniProt/SwissProt
Match: sp|Q568Y3|RN185_RAT (E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 1.168e-51
Identity = 93/176 (52.84%), Postives = 112/176 (63.64%), Query Frame = 1

Query:  160 ETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            E+ G +S+  F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   29 ESGGQDST--FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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BLAST of E3 ubiquitin-ligase RNF185-like vs. UniProt/SwissProt
Match: sp|Q96GF1|RN185_HUMAN (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 1.168e-51
Identity = 93/176 (52.84%), Postives = 112/176 (63.64%), Query Frame = 1

Query:  160 ETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            E+ G +S+  F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   29 ESGGQDST--FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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BLAST of E3 ubiquitin-ligase RNF185-like vs. UniProt/SwissProt
Match: sp|Q5RFK9|RN185_PONAB (E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 2.387e-51
Identity = 93/176 (52.84%), Postives = 112/176 (63.64%), Query Frame = 1

Query:  160 ETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            E+ G +S+  F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   29 ESGGQDST--FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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BLAST of E3 ubiquitin-ligase RNF185-like vs. UniProt/SwissProt
Match: sp|Q5ZIR9|RN185_CHICK (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 3.424e-51
Identity = 91/171 (53.22%), Postives = 108/171 (63.16%), Query Frame = 1

Query:  175 NSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            N   +F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:   34 NQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 190

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BLAST of E3 ubiquitin-ligase RNF185-like vs. TrEMBL
Match: A0A1W4X226 (E3 ubiquitin-protein ligase RNF185-like OS=Agrilus planipennis OX=224129 GN=LOC108740347 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 5.142e-53
Identity = 99/201 (49.25%), Postives = 121/201 (60.20%), Query Frame = 1

Query:  100 MAESTNTSNSGFPSGSGTGPETAGNNSSG--SFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGV---DTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            MA S N S    PS S  G    G++     +F+CNICL+ +KD V+SMCGHLFCWPCLH+W+ET+ SR  CPVCKAAIS+DKVIPLYGR ST+Q+DPR+K+PPRP GQRTE +                 PG    D G +MSFGIGAFP G F +TFNF         PN          + + LSK+FL +A     W
Sbjct:    1 MASSKNESE---PSSSKAGVSGEGDDKKEDRTFECNICLDTAKDAVVSMCGHLFCWPCLHQWLETRLSRQVCPVCKAAISKDKVIPLYGRGSTNQQDPREKVPPRPAGQRTEPESGSNF------------PGFGFGDNGFHMSFGIGAFPFGFFTSTFNFG-----DVRPNAAPLGTPQHMEEQFLSKIFLWLAFLFIAW 181

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BLAST of E3 ubiquitin-ligase RNF185-like vs. TrEMBL
Match: A0A087UTJ1 (E3 ubiquitin-protein ligase RNF185 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_10572 PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 5.931e-53
Identity = 97/186 (52.15%), Postives = 120/186 (64.52%), Query Frame = 1

Query:  145 SGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGV---DTGVNMSFGIGAFPLGLFATTFNFTGGN--NPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            SG G E       G+F+CNICL+ +KD V+S+CGHLFCWPCLHRW+ET+ SR  CPVCKAAISR+KVIPLYGR  ++Q DPR+++PPRP GQRTE + +               PG    DTG +MSFGIGAFP G+FA++FNF  G    P   P F E         +LLSKVFL +A    +W
Sbjct:    8 SGGGSEET-RTQEGTFECNICLDTAKDAVVSLCGHLFCWPCLHRWLETRPSRQVCPVCKAAISREKVIPLYGRGGSNQ-DPRERLPPRPSGQRTEPENHAGQSF----------PGFGFGDTGFHMSFGIGAFPFGIFASSFNFGDGRPAPPPGTPEFEE--------DQLLSKVFLYVAIAFIVW 173

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BLAST of E3 ubiquitin-ligase RNF185-like vs. TrEMBL
Match: A0A1S3A4N2 (E3 ubiquitin-protein ligase RNF185 isoform X1 OS=Erinaceus europaeus OX=9365 GN=RNF185 PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 6.421e-53
Identity = 101/195 (51.79%), Postives = 124/195 (63.59%), Query Frame = 1

Query:  103 AESTNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            + S   S++G PSG+G G E+ G +S+  F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:    9 SASPENSSAGGPSGNGAG-ESGGQDST--FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 186

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BLAST of E3 ubiquitin-ligase RNF185-like vs. TrEMBL
Match: A0A3M7QLX5 (E3 ubiquitin-ligase RNF185-like OS=Brachionus plicatilis OX=10195 GN=BpHYR1_035672 PE=4 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 1.164e-52
Identity = 91/161 (56.52%), Postives = 114/161 (70.81%), Query Frame = 1

Query:  139 SGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNN----PQNDPNFRE 609
            S SG   E + +N+S  ++CNICLE +KD VISMCGHLFCWPCLH+W+ET+    TCPVCK+AI++DKVIPLYGR+S+++ DPRDKIPPRPQGQRTE+ RN F              G+D G+++SFGIGAFP   FA+TFNFT  N     P   P + E
Sbjct:    9 SKSGKNNENSSDNASSQYECNICLETAKDAVISMCGHLFCWPCLHKWLETKPLNQTCPVCKSAINKDKVIPLYGRNSSNKTDPRDKIPPRPQGQRTEAPRNNFFAS-----------GLD-GLHVSFGIGAFPFSFFASTFNFTTDNRMPAPPPGSPQYAE 157

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BLAST of E3 ubiquitin-ligase RNF185-like vs. TrEMBL
Match: H9Z0M5 (RING finger protein 185 isoform 1 OS=Macaca mulatta OX=9544 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 2.059e-52
Identity = 100/195 (51.28%), Postives = 120/195 (61.54%), Query Frame = 1

Query:  103 AESTNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            + S   S +G PSGS  G   +G   S +F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR+K PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  Y  +Q   LS++FL +A     W
Sbjct:    9 SASPENSTAGSPSGSSNGAGESGGQDS-TFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPG---TPQYVDEQ--FLSRLFLFVALVIMFW 188

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Cavefish
Match: rnf185 (ring finger protein 185 [Source:NCBI gene;Acc:406310])

HSP 1 Score: 181.03 bits (458), Expect = 6.357e-55
Identity = 93/186 (50.00%), Postives = 117/186 (62.90%), Query Frame = 1

Query:  139 SGSGTGPETAGNNSS--GSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGV-DTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            + +G+  + +G +SS   +F+CNICL+ SKD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR++ PPRPQGQR E +               VR G  D G  MSFGIGAFP G+FAT FN   G  P   P   +         + LS++FL +A     W
Sbjct:   18 TATGSSGQASGESSSQDSTFECNICLDTSKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPRERTPPRPQGQRPEPEN------------RGVRFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAAPGTPQH-----MDEQFLSRLFLFVALVIMFW 186

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Cavefish
Match: RNF5 (ring finger protein 5 [Source:NCBI gene;Acc:103046714])

HSP 1 Score: 170.244 bits (430), Expect = 8.212e-51
Identity = 91/176 (51.70%), Postives = 106/176 (60.23%), Query Frame = 1

Query:  100 MAESTNTSNSGFPSGSGTGP-ETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYS 624
            MA +    + G     G G  E  G+    +F+CNICL+ ++D VIS+CGHLFCWPCLH+W+ET+ SR  CPVCKA ISRDKVIPLYGR ST Q DPR K PPRPQGQRTE +  G                 DTG +MSFGIGAFP G F T FN        NDP  R    Y+
Sbjct:    1 MAAAAEPRSGGEGHHPGDGDRERDGDGDRAAFECNICLDTARDAVISLCGHLFCWPCLHQWLETRPSRQQCPVCKAGISRDKVIPLYGRGSTSQEDPRLKTPPRPQGQRTEPESRGPFHGFG-----------DTGFHMSFGIGAFPFGFFTTVFN-------TNDPFHRADPHYA 158

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Cavefish
Match: ENSAMXT00000052741.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000182.1:466606:473602:1 gene:ENSAMXG00000043276.1 transcript:ENSAMXT00000052741.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.6026 bits (122), Expect = 8.285e-7
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 1

Query:  151 TGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKA 324
            T P    N +S +  C+ICLE+  D V + CGH FC  CL ++ ++ SSR  CPVCKA
Sbjct:    2 TLPNNVQNLTSEAKPCSICLEVFNDPVSTPCGHNFCVVCLRQYWDS-SSRCQCPVCKA 58

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Cavefish
Match: pex10 (peroxisomal biogenesis factor 10 [Source:NCBI gene;Acc:103034252])

HSP 1 Score: 50.8322 bits (120), Expect = 1.290e-6
Identity = 22/70 (31.43%), Postives = 36/70 (51.43%), Query Frame = 1

Query:  151 TGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYG 360
            + P+   + SS +  C +CLE  +    + CGHLFCW C+  W  T   +  CP+C+      +++ L G
Sbjct:  257 SSPQAVRSPSSKTSRCILCLEERRHPTTTPCGHLFCWECITEWCNT---KMECPLCREKFQPHRLVYLRG 323

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BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Cavefish
Match: hltf (helicase-like transcription factor [Source:ZFIN;Acc:ZDB-GENE-030131-3306])

HSP 1 Score: 50.8322 bits (120), Expect = 2.021e-6
Identity = 24/68 (35.29%), Postives = 40/68 (58.82%), Query Frame = 1

Query:  193 DCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDK 396
            +C +CL+  +  VI+ C H+FC PC+   I+++   A CP+C+A I   +++  Y  D  D R P D+
Sbjct:  731 ECAVCLDSLRQPVITHCAHVFCKPCICEVIQSKQLAAKCPLCRANIKSTELLE-YPGDEEDSR-PTDR 796

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Yeast
Match: IRC20 (E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci [Source:SGD;Acc:S000004237])

HSP 1 Score: 52.373 bits (124), Expect = 9.982e-8
Identity = 27/87 (31.03%), Postives = 43/87 (49.43%), Query Frame = 1

Query:  175 NSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESD 435
            N +    C+ICL   +   I  CGH FC  C+  W+   S    CP+CK   S  +V     ++ST++R+ ++   PR +G  +  D
Sbjct: 1232 NDNQILSCSICLGEVEIGAIIKCGHYFCKSCILTWLRAHSK---CPICKGFCSISEVYNFKFKNSTEKRE-KEIQEPRREGADSSQD 1314

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Nematostella
Match: EDO37344 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFY7])

HSP 1 Score: 168.318 bits (425), Expect = 1.413e-50
Identity = 86/177 (48.59%), Postives = 114/177 (64.41%), Query Frame = 1

Query:  157 PETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            P    N+++ +F+CNICL+ ++D VISMCGHLFCWPCLHRW+ET+ +R+ CPVCKA IS++KVIPL+GR S+  +DPR+K+PPRPQ QRTE + NG               G   G  +SFGIGAFP   F+TT    GGNNPQ+        +   +  + LSK+FL +A     W
Sbjct:   22 PSEDPNSANANFECNICLDTARDAVISMCGHLFCWPCLHRWLETRPNRSMCPVCKAGISKEKVIPLFGRGSSSNQDPREKMPPRPQAQRTEPENNGTFAGFG---------GFGDGFQLSFGIGAFPFAFFSTTL---GGNNPQHP---APPGSPQAEDEQFLSKLFLWVAFLFMFW 183

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Nematostella
Match: EDO40992 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5K6])

HSP 1 Score: 51.6026 bits (122), Expect = 2.337e-7
Identity = 21/72 (29.17%), Postives = 37/72 (51.39%), Query Frame = 1

Query:  157 PETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDST 372
            P     +  G+  C++CLE  K    + CGHLFCW C+  W  ++     CP+C+  +   +++ L+  + T
Sbjct:  229 PSVVPQSMPGTLKCSLCLENVKHITSTSCGHLFCWHCITEWCSSK-----CPLCREPLQMSRLVYLHHFEPT 295

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Nematostella
Match: EDO30997 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZ68])

HSP 1 Score: 42.3578 bits (98), Expect = 9.526e-6
Identity = 18/46 (39.13%), Postives = 28/46 (60.87%), Query Frame = 1

Query:  196 CNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAIS 333
            C IC E+    + + C H FC  C+  W++   +++ CPVCKA+IS
Sbjct:   16 CAICKEVLTQPIATPCDHYFCVLCICEWLDQTYNKSGCPVCKASIS 61

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Medaka
Match: rnf185 (ring finger protein 185 [Source:NCBI gene;Acc:101167555])

HSP 1 Score: 170.244 bits (430), Expect = 8.516e-51
Identity = 88/167 (52.69%), Postives = 108/167 (64.67%), Query Frame = 1

Query:  187 SFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            +F+CNICL+ +KD VIS+CGHLFCWPCLH+W+ET+ +R  CPVCKA ISRDKVIPLYGR ST Q+DPR++ PPRPQGQR E +  G                 D G  MSFGIGAFP G+FAT FN   G  P   P    T  ++ +Q   LS++FL +A     W
Sbjct:   36 TFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPRERTPPRPQGQRPEPENRGGFQGFGF---------GDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAAPG---TPQHTDEQ--FLSRLFLFVALVIMFW 188

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Medaka
Match: RNF5 (ring finger protein 5 [Source:NCBI gene;Acc:101175247])

HSP 1 Score: 168.703 bits (426), Expect = 3.718e-50
Identity = 92/181 (50.83%), Postives = 106/181 (58.56%), Query Frame = 1

Query:  103 AESTNTSNSGFPSGSGTGPE-------TAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYS 624
            A     S  GFP+G  +                  +F+CNICL+ ++D VISMCGHLFCWPCLH+W+ETQ SR  CPVCKA ISR+KVIPLYGR S+ Q DPR K PPRPQGQRTE +  G    G            DTG +MSFGIGAFP G F T FN        NDP F   D Y+
Sbjct:    3 AADPRYSRGGFPAGESSNDRDGPGGGSGESERDRATFECNICLDTARDAVISMCGHLFCWPCLHQWLETQPSRQQCPVCKAGISREKVIPLYGRGSSSQEDPRLKTPPRPQGQRTEPESRGGMFQGFG----------DTGFHMSFGIGAFPFGFFTTVFN-------ANDP-FHRADQYA 165

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Medaka
Match: RNF5 (ring finger protein 5 [Source:NCBI gene;Acc:101175247])

HSP 1 Score: 131.724 bits (330), Expect = 3.602e-36
Identity = 82/179 (45.81%), Postives = 96/179 (53.63%), Query Frame = 1

Query:  103 AESTNTSNSGFPSGSGTGPE-------TAGNNSSGSFDCNICLEISKDTVISMCGHLFCWP-CLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETD 615
            A     S  GFP+G  +                  +F+CNICL+ ++D VISM      +P C  +W+ETQ SR  CPVCKA ISR+KVIPLYGR S+ Q DPR K PPRPQGQRTE +  G    G            DTG +MSFGIGAFP G F T FN        NDP  R  D
Sbjct:    3 AADPRYSRGGFPAGESSNDRDGPGGGSGESERDRATFECNICLDTARDAVISM------YPSCPSQWLETQPSRQQCPVCKAGISREKVIPLYGRGSSSQEDPRLKTPPRPQGQRTEPESRGGMFQGFG----------DTGFHMSFGIGAFPFGFFTTVFN-------ANDPFHRAGD 158

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Medaka
Match: rfwd3 (ring finger and WD repeat domain 3 [Source:NCBI gene;Acc:101171660])

HSP 1 Score: 50.447 bits (119), Expect = 2.382e-6
Identity = 23/60 (38.33%), Postives = 32/60 (53.33%), Query Frame = 1

Query:  196 CNICLE---ISKDTVISM--CGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYG 360
            C IC E    + +  +S   CGHLF + C+HRW++ Q S A CP C     R  ++ LY 
Sbjct:  208 CTICFESWTTAGEHRLSALRCGHLFGYTCIHRWLKAQGSAAKCPQCNKKAKRSDIVLLYA 267

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Medaka
Match: hltf (helicase like transcription factor [Source:NCBI gene;Acc:101157336])

HSP 1 Score: 49.6766 bits (117), Expect = 4.494e-6
Identity = 19/68 (27.94%), Postives = 40/68 (58.82%), Query Frame = 1

Query:  178 SSGS-FDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQ 378
            +SGS  +C++CL+  +  VI+ C H++C PC+ + I  +  +  CP+C++ I   +++     D  ++
Sbjct:  788 ASGSDEECSVCLDSIRLPVITHCAHVYCRPCIAQVISNEQEKPRCPLCRSEIKSSELVEFPQEDMEEE 855

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Planmine SMEST
Match: SMESG000060298.1 (SMESG000060298.1)

HSP 1 Score: 370.163 bits (949), Expect = 7.448e-129
Identity = 195/196 (99.49%), Postives = 195/196 (99.49%), Query Frame = 1

Query:  100 MAESTNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRXXXXXXXXXXXXXXVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 687
            MAESTNTSNSGFPSGSG GPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRNGFSGIGNIFNGFNVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW
Sbjct:    1 MAESTNTSNSGFPSGSGAGPETAGNNSSGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESDRNGFSGIGNIFNGFNVRPGVDTGVNMSFGIGAFPLGLFATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIW 196

[Close]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Planmine SMEST
Match: SMESG000016669.1 (SMESG000016669.1)

HSP 1 Score: 111.309 bits (277), Expect = 1.025e-28
Identity = 48/86 (55.81%), Postives = 64/86 (74.42%), Query Frame = 1

Query:  181 SGSFDCNICLEISKDTVISMCGHLFCWPCLHRWIETQ-SSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKIPPRPQGQRTESD 435
            + + DCNICLE S  +V++ CGH+FCWPC+  WIE Q S+   CPVCKA IS DK+IP+YG++S +Q DPR+ IPPRP  Q +E +
Sbjct:   13 NSNLDCNICLEESTQSVVTYCGHVFCWPCIFTWIEVQNSTENICPVCKAVISVDKLIPIYGQNS-NQIDPRNTIPPRPLAQYSEQN 97

The following BLAST results are available for this feature:

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Human
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Celegans
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Fly
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Zebrafish
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Xenopus
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Mouse
BLAST of E3 ubiquitin-ligase RNF185-like vs. UniProt/SwissProt
BLAST of E3 ubiquitin-ligase RNF185-like vs. TrEMBL
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Cavefish
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Sea Lamprey
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Yeast
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Nematostella
BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Medaka
BLAST of E3 ubiquitin-ligase RNF185-like vs. Planmine SMEST

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RNF185	2.432e-52	52.84	ring finger protein 185 [Source:HGNC Symbol;Acc:HG...	[more]
RNF185	2.432e-52	52.84	ring finger protein 185 [Source:HGNC Symbol;Acc:HG...	[more]
RNF5	2.147e-43	50.00	ring finger protein 5 [Source:HGNC Symbol;Acc:HGNC...	[more]
RNF5	2.147e-43	50.00	ring finger protein 5 [Source:HGNC Symbol;Acc:HGNC...	[more]
RNF5	2.147e-43	50.00	ring finger protein 5 [Source:HGNC Symbol;Acc:HGNC...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
rnf-5	1.519e-34	48.12	E3 ubiquitin ligase rnf-5 [Source:UniProtKB/Swiss...	[more]
rnf-5	9.287e-32	55.06	E3 ubiquitin ligase rnf-5 [Source:UniProtKB/Swiss...	[more]
C32D5.11	6.316e-6	37.88	pep chromosome:WBcel235:II:6352542:6355911:-1 gene...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CG8974	2.637e-48	46.15	gene:FBgn0030693 transcript:FBtr0074188	[more]
CG8974	2.637e-48	46.15	gene:FBgn0030693 transcript:FBtr0074186	[more]
CG8974	2.637e-48	46.15	gene:FBgn0030693 transcript:FBtr0074187	[more]
CG8974	2.637e-48	46.15	gene:FBgn0030693 transcript:FBtr0074185	[more]
CG8974	2.637e-48	46.15	gene:FBgn0030693 transcript:FBtr0074189	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rnf185	2.419e-51	53.89	ring finger protein 185 [Source:NCBI gene;Acc:4063...	[more]
RNF5	9.335e-36	49.33	ring finger protein 5 [Source:NCBI gene;Acc:100537...	[more]
hltf	7.968e-8	32.86	helicase-like transcription factor [Source:ZFIN;Ac...	[more]
hltf	8.191e-8	32.86	helicase-like transcription factor [Source:ZFIN;Ac...	[more]
rnf185	2.848e-6	75.00	ring finger protein 185 [Source:NCBI gene;Acc:4063...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
supt16h	1.097e-47	48.21	SPT16 homolog, facilitates chromatin remodeling su...	[more]
RNF185	1.505e-42	46.11	ring finger protein 185 [Source:HGNC Symbol;Acc:HG...	[more]
HLTF	6.611e-7	37.93	helicase-like transcription factor [Source:NCBI ge...	[more]
HLTF	7.038e-7	37.93	helicase-like transcription factor [Source:NCBI ge...	[more]
chrng	1.806e-6	37.04	cholinergic receptor, nicotinic gamma [Source:Xenb...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Rnf185	3.518e-57	52.31	ring finger protein 185 [Source:MGI Symbol;Acc:MGI...	[more]
Rnf185	6.289e-56	52.31	ring finger protein 185 [Source:MGI Symbol;Acc:MGI...	[more]
Rnf5	1.821e-43	50.35	ring finger protein 5 [Source:MGI Symbol;Acc:MGI:1...	[more]
Brca1	3.319e-6	34.69	breast cancer 1, early onset [Source:MGI Symbol;Ac...	[more]
Hltf	3.518e-6	39.66	helicase-like transcription factor [Source:MGI Sym...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q91YT2\|RN185_MOUSE	2.461e-56	52.31	E3 ubiquitin-protein ligase RNF185 OS=Mus musculus...	[more]
sp\|Q568Y3\|RN185_RAT	1.168e-51	52.84	E3 ubiquitin-protein ligase RNF185 OS=Rattus norve...	[more]
sp\|Q96GF1\|RN185_HUMAN	1.168e-51	52.84	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens...	[more]
sp\|Q5RFK9\|RN185_PONAB	2.387e-51	52.84	E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii...	[more]
sp\|Q5ZIR9\|RN185_CHICK	3.424e-51	53.22	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallu...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1W4X226	5.142e-53	49.25	E3 ubiquitin-protein ligase RNF185-like OS=Agrilus...	[more]
A0A087UTJ1	5.931e-53	52.15	E3 ubiquitin-protein ligase RNF185 (Fragment) OS=S...	[more]
A0A1S3A4N2	6.421e-53	51.79	E3 ubiquitin-protein ligase RNF185 isoform X1 OS=E...	[more]
A0A3M7QLX5	1.164e-52	56.52	E3 ubiquitin-ligase RNF185-like OS=Brachionus plic...	[more]
H9Z0M5	2.059e-52	51.28	RING finger protein 185 isoform 1 OS=Macaca mulatt...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rnf185	6.357e-55	50.00	ring finger protein 185 [Source:NCBI gene;Acc:4063...	[more]
RNF5	8.212e-51	51.70	ring finger protein 5 [Source:NCBI gene;Acc:103046...	[more]
ENSAMXT00000052741.1	8.285e-7	46.55	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
pex10	1.290e-6	31.43	peroxisomal biogenesis factor 10 [Source:NCBI gene...	[more]
hltf	2.021e-6	35.29	helicase-like transcription factor [Source:ZFIN;Ac...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
IRC20	9.982e-8	31.03	E3 ubiquitin ligase and putative helicase; involve...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3


Match Name	E-value	Identity	Description
EDO37344	1.413e-50	48.59	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO40992	2.337e-7	29.17	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO30997	9.526e-6	39.13	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rnf185	8.516e-51	52.69	ring finger protein 185 [Source:NCBI gene;Acc:1011...	[more]
RNF5	3.718e-50	50.83	ring finger protein 5 [Source:NCBI gene;Acc:101175...	[more]
RNF5	3.602e-36	45.81	ring finger protein 5 [Source:NCBI gene;Acc:101175...	[more]
rfwd3	2.382e-6	38.33	ring finger and WD repeat domain 3 [Source:NCBI ge...	[more]
hltf	4.494e-6	27.94	helicase like transcription factor [Source:NCBI ge...	[more]

BLAST of E3 ubiquitin-ligase RNF185-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000060298.1	7.448e-129	99.49	SMESG000060298.1	[more]
SMESG000016669.1	1.025e-28	55.81	SMESG000016669.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30020665 ID=SMED30020665|Name=E3 ubiquitin-ligase RNF185-like|organism=Schmidtea mediterranea sexual|type=transcript|length=1253bp
AATGTTTAAAAAATTTATTAATTATATTATATTGTCTCTGTAATTAAATT
TTTAAATATAATTGATTAAACAATTATAGCCGATTTTAGCAAAAATTAAA
TGGCTGAAAGTACAAATACTAGTAATTCAGGTTTTCCTAGTGGATCTGGA
ACAGGTCCCGAAACAGCAGGAAATAATTCGAGTGGTTCTTTTGATTGTAA
TATTTGTTTGGAAATATCTAAAGATACAGTTATTAGTATGTGTGGGCATT
TATTTTGTTGGCCATGTCTGCATCGGTGGATAGAAACCCAAAGTTCACGT
GCAACATGCCCTGTCTGTAAAGCAGCAATAAGCAGGGATAAAGTGATTCC
CTTGTACGGTCGTGATAGCACAGATCAAAGAGATCCTAGAGATAAAATTC
CTCCACGGCCTCAAGGTCAACGTACCGAATCCGACAGAAATGGATTTAGC
GGCATTGGTAATATATTTAATGGATTCAATGTCAGGCCAGGTGTTGATAC
CGGAGTCAACATGTCCTTTGGCATAGGAGCTTTTCCTCTCGGTCTTTTTG
CAACCACATTTAATTTCACCGGCGGAAACAATCCTCAAAATGATCCTAAT
TTTAGAGAAACAGATGCCTATTCTATTCAGCAAAGCGAATTATTATCCAA
AGTGTTTTTACTAATCGCAGGATGTTGTTTCATTTGGCTTTTATTAGCGT
GATATTTACCGTTAAAGATACTATTATAATTTAAAAGGCAAATAAATTAC
TGATCATTTATAAATTTCAATTTAAATTTATGCATATTTTAAAATTTTGT
GCAATTCACAATTTAATTACTATAATTGTTTAATTAAATAACTTGTGTTT
GTTTGTAATGAATTTGGCGGTAATTTATTTTTAATAAAACATGGTAATCA
TGTTAATTTTTTAACAAAAATACTTAGTTAACATTTATTGATAATGTTCT
ACTGTTGTGTATAGAATAAAACATGGTAATCATCTTTATTATTATTTTAG
CGTAACAAAATACATCATCTACATAGATGAACCTAACCCAATGTTTTGTA
CTCTGTCAGACTTCAGTACTGTTCGCGACAAAAACATGAATGGTATTTGT
GTTCCATGTGCATGGTGTACATATTATGTACATCAGTTTTGTGCCATAAA
TTAAATTTCTTGACTTATAAAGTTAGGAAAACAATTATTTTTGTAATTTA
AATTGTCCATTTTATAACTGTTTGACTATTAAATTGTAGTCTTATTACAA
AAA

protein sequence of SMED30020665-orf-1

>SMED30020665-orf-1 ID=SMED30020665-orf-1|Name=SMED30020665-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=201bp
MAESTNTSNSGFPSGSGTGPETAGNNSSGSFDCNICLEISKDTVISMCGH
LFCWPCLHRWIETQSSRATCPVCKAAISRDKVIPLYGRDSTDQRDPRDKI
PPRPQGQRTESDRNGFSGIGNIFNGFNVRPGVDTGVNMSFGIGAFPLGLF
ATTFNFTGGNNPQNDPNFRETDAYSIQQSELLSKVFLLIAGCCFIWLLLA
*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0016874	ligase activity
GO:0008270	zinc ion binding
GO:0005515	protein binding
GO:0016740	transferase activity
GO:0044390	ubiquitin-like protein conjugating enzyme binding
GO:0046872	metal ion binding
GO:1904264	ubiquitin protein ligase activity involved in ERAD pathway

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000101	muscle cell
PLANA:0000142	posterior region of the whole animal

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD
IPR017907	Znf_RING_CS

Vocabulary: **cellular component**
Term	Definition
GO:0005739	mitochondrion
GO:0005741	mitochondrial outer membrane
GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0036513	Derlin-1 retrotranslocation complex

Vocabulary: **biological process**
Term	Definition
GO:0006914	autophagy
GO:0016567	protein ubiquitination
GO:0030433	ubiquitin-dependent ERAD pathway
GO:0036503	ERAD pathway
GO:0042787	protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0051865	protein autoubiquitination
GO:0071712	ER-associated misfolded protein catabolic process

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 33..73 e-value: 2.0E-8 score: 44.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 33..74 score: 13.64
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 32..78 e-value: 7.0E-10 score: 38.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..106
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..114
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR12313	RNF5	coord: 3..198
None	No IPR available	PANTHER	PTHR12313:SF13	E3 UBIQUITIN-PROTEIN LIGASE RNF185	coord: 3..198
None	No IPR available	CDD	cd16534	RING-HC_RNF5_like	coord: 32..74 e-value: 1.11369E-24 score: 89.0284
None	No IPR available	SUPERFAMILY	SSF57850	RING/U-box	coord: 30..86
None	No IPR available	TMHMM	TMhelix		coord: 135..157
None	No IPR available	TMHMM	TMhelix		coord: 177..199
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 20..87 e-value: 2.2E-18 score: 67.4
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 48..57

Main menu

E3 ubiquitin-ligase RNF185-like

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Owlery

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