Mitochondrial ribosome protein 63
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020645 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of Mitochondrial ribosome protein 63 vs. TrEMBL
Match: A0A0R3W8L7 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS6718 PE=4 SV=1) HSP 1 Score: 110.538 bits (275), Expect = 7.017e-28 Identity = 54/115 (46.96%), Postives = 75/115 (65.22%), Query Frame = 2 Query: 53 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNYE 397 MR++Y +LG F+PYK+VPGSLWAGKQRKIP LT RKA +D +L+ QN + LS P+ISKE E+ L E+ +EL ++ + Y F RFPN L+ FW+ + K ++ Sbjct: 1 MRLTYFLLGHFIPYKRVPGSLWAGKQRKIPRLTASRKAAFMDELLMTQQNERYLSKPFISKEAEATTLP-AEQAKELAAENEVFYKIYEEKFRIRFPNRKLENFWSHLNNSKKFD 114
BLAST of Mitochondrial ribosome protein 63 vs. TrEMBL
Match: A0A0R3XCJ6 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS11256 PE=4 SV=1) HSP 1 Score: 110.153 bits (274), Expect = 9.202e-28 Identity = 51/115 (44.35%), Postives = 75/115 (65.22%), Query Frame = 2 Query: 53 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNYE 397 MR+++ + GRF+PYK+VPGSLWAGKQRKIP LT RKA +D +L+ QN + LS P+I +E E+ L E+ +EL + ++ + Y F RFPN L+ FW+ + K ++ Sbjct: 1 MRLTFLLSGRFIPYKRVPGSLWAGKQRKIPRLTASRKAAFMDELLLAQQNERYLSKPFIGQEAEATTLP-AEQAKELAKENETFYRIYEAKFRTRFPNRRLEHFWSHLNVTKKFD 114
BLAST of Mitochondrial ribosome protein 63 vs. TrEMBL
Match: A0A3P7LNY8 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS8494 PE=4 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 1.451e-27 Identity = 50/114 (43.86%), Postives = 75/114 (65.79%), Query Frame = 2 Query: 53 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNY 394 MR+S+++LGRF+P++ VPGSLW GK+RKIP LT RKA +D +L+ QN + LSNPY+S E E+ L E+ RE+ D + Y + F +RFP+ L+ FW+ + + + Sbjct: 1 MRLSFSLLGRFIPWRSVPGSLWGGKRRKIPRLTGARKAAFMDELLLTQQNERYLSNPYLSVEAEATTLA-AEQAREIAAEDRVFYKVYEDKFRKRFPDRKLENFWSHLNTTRKF 113
BLAST of Mitochondrial ribosome protein 63 vs. TrEMBL
Match: A0A4Z2DCF1 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002417 PE=4 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 1.515e-27 Identity = 55/115 (47.83%), Postives = 72/115 (62.61%), Query Frame = 2 Query: 53 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNYE 397 M ++ A GRFV +K++PGS+W GKQRKIPLLTN RK LD +L+ QN L PY ++EVESA L EK REL+ D Y FN RFP +++ FW+ + K K Y+ Sbjct: 1 MILTAACYGRFVRWKRIPGSVWGGKQRKIPLLTNSRKEAFLDELLISHQNHMYLQKPYFTEEVESATLAE-EKARELQMEDRFFYDRYAEQFNRRFPTRNIEAFWDKLYKTKRYD 114
BLAST of Mitochondrial ribosome protein 63 vs. TrEMBL
Match: W6URX7 (Uncharacterized protein OS=Echinococcus granulosus OX=6210 GN=EGR_01116 PE=4 SV=1) HSP 1 Score: 110.153 bits (274), Expect = 2.354e-27 Identity = 53/115 (46.09%), Postives = 76/115 (66.09%), Query Frame = 2 Query: 53 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNYE 397 MR++ +LG F+PYK+VPGSLWAGKQRKIP LT RKA +D +L+ QN + LS P++S+E E+ L EK +EL R ++ + Y F RFPN L++FW+ + K ++ Sbjct: 35 MRLTSLLLGHFIPYKRVPGSLWAGKQRKIPRLTASRKAAFMDELLLTQQNERYLSRPFLSQEAEATTLP-AEKAKELARENEVFYKIYEEKFRIRFPNRKLEDFWSHLNNCKKFD 148
BLAST of Mitochondrial ribosome protein 63 vs. Planmine SMEST
Match: SMESG000077978.1 (SMESG000077978.1) HSP 1 Score: 237.269 bits (604), Expect = 2.555e-81 Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 2 Query: 53 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNYEN 400 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNYEN Sbjct: 1 MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNLKILSNPYISKEVESAYLELIEKPRELKRIDDNIIQNYVNSFNERFPNTSLDEFWNCIKKYKNYEN 116 The following BLAST results are available for this feature:
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Mitochondrial ribosome protein 63 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020645 ID=SMED30020645|Name=Mitochondrial ribosome protein 63|organism=Schmidtea mediterranea sexual|type=transcript|length=566bpback to top protein sequence of SMED30020645-orf-1 >SMED30020645-orf-1 ID=SMED30020645-orf-1|Name=SMED30020645-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=117bp MRISYAVLGRFVPYKQVPGSLWAGKQRKIPLLTNYRKAGMLDRILVEDQNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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