Usp domain-containing protein

Overview
NameUsp domain-containing protein
Smed IDSMED30020630
Length (bp)630
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Usp domain-containing protein (SMED30020630) t-SNE clustered cells

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30020630) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Usp domain-containing protein (SMED30020630) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30020630) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020630

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Category 3 cellSMED30020630SMESG000068262.1 SMESG000053435.1 SMESG000049288.1 dd_Smed_v6_6797_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Match: sp|P74148|Y1388_SYNY3 (Universal stress protein Sll1388 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1388 PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.219e-10
Identity = 30/90 (33.33%), Postives = 50/90 (55.56%), Query Frame = 3 
Query:  285 VMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVH 554
            +++  +++ Q E        + Q +   ++C+W  K G+P + I      ++ADLVV+G RGL  +    LGSVS+YV+HHV    +IV 
Sbjct:   64 IIKEHLEEQQTEAREWLQSIVQQVQEDGVACEWDVKVGEPGRWIRDMAKNWDADLVVLGRRGLKGLAEVFLGSVSSYVIHHVQCSVLIVQ 153
[Close]
BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8LGG8|USPAL_ARATH (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 58.9214 bits (141), Expect = 9.514e-10
Identity = 39/140 (27.86%), Postives = 64/140 (45.71%), Query Frame = 3 
Query:  141 SIYSKYAFQWYLKNSPEVKTD--QIILLHSMTPPGDVEISEDGNLKVD-LLVMENDMKKIQKEMES----LKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIP 539
            SI  K AF+W L+      T   +I+LLH         + EDG   VD +     D + +++  ++    L   F+++C    + C+   K G P   I   V +   D +V+GSRGL    +  +G+VS + + H   P
Sbjct:   24 SISCKRAFEWTLEKIVRSNTSDFKILLLHVQV------VDEDGFDDVDSIYASPEDFRDMRQSNKAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEVKRVRPDFLVVGSRGLGRFQKVFVGTVSAFCVKHAECP 157
[Close]
BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Match: sp|P72745|Y1101_SYNY3 (Universal stress protein Slr1101 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1101 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.693e-6
Identity = 27/97 (27.84%), Postives = 46/97 (47.42%), Query Frame = 3 
Query:  291 ENDMKKIQKEMESLKSVFMDQCKNRK-------LSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAP 560
            E  M+  Q++ ++ +   +D  + R+       + CQ     G P + I     Q N+D++V+G RG   +   +LGSV  YV HH +    +V  P
Sbjct:   11 ELTMEVWQQQWQTFEQEGLDTLEKRRQQALALDIECQAEQILGSPGKIICQRAKQDNSDIIVVGHRGRWGLSEILLGSVGNYVFHHAHCCVFVVPTP 107
[Close]
BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Match: sp|A0QZA1|Y3950_MYCS2 (Universal stress protein MSMEG_3950/MSMEI_3859 OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_3950 PE=1 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 5.695e-6
Identity = 39/148 (26.35%), Postives = 73/148 (49.32%), Query Frame = 3 
Query:  120 VLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQC--KNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHA 557
            +L+ +D S  S  A +W  + +  +    I L+H + P   V +S      +   V+E   +  +  +E  + V  D C  +   L+ Q   +    A+ ++ A    +A +VV+G+RG+  + R +LGS ST +LH+ + P ++VH 
Sbjct:   10 ILVGVDSSAESDAAVRWAAREA-SLHDAPITLMHVIAP---VVVSWPAGPYM-ATVLECQEENARHAIEQAQKVVAD-CLGETHGLTVQTEIRKESVARTLIDA--SKSAQMVVVGNRGMGALGRVLLGSTSTSLLHYASGPVVVVHG 149
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A1I8GWE6 (Usp domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019000g3 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.011e-23
Identity = 57/149 (38.26%), Postives = 85/149 (57.05%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPG---DVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
            + VL+A+D S  ++ AF  Y  + P  ++D IILLHSM  P     + +S  G    D    E   +++++  + L    M QC+ R+L C++VS+ G P   IV A  ++ A++VV+G RG N I R ILGSVS YVL H   P ++V
Sbjct:   31 RRVLIAVDGSKGAQRAFD-YWSSMPGSQSDSIILLHSMEAPNVLTGLLLSPSGTF--DYEEYEKQGRQLKQRSQQLSQELMAQCEKRRLHCKFVSEAGTPGATIVEAARKYAANVVVVGCRGNNGIRRTILGSVSDYVLQHSCRPVVVV 176
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A267H1T1 (Usp domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019000g6 PE=4 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 8.853e-23
Identity = 56/149 (37.58%), Postives = 85/149 (57.05%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPG---DVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
            + VL+A+D S  ++ AF  Y  + P  ++D IILLHS+  P     + +S  G    D    E   +++++  + L    M QC+ R+L C++VS+ G P   IV A  ++ A++VV+G RG N I R ILGSVS YVL H   P ++V
Sbjct:   31 RRVLIAVDGSKGAQRAFD-YWSSIPGSQSDSIILLHSVEAPNVLTGLLLSPSGTF--DYEEYEKQGRQLKQRSQQLSQELMAQCEKRRLHCKFVSEAGTPGATIVEAARKYAANVVVVGCRGNNGIRRTILGSVSDYVLQHSCRPVVVV 176
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A1I8IW26 (Usp domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 8.433e-22
Identity = 56/154 (36.36%), Postives = 85/154 (55.19%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLV--------MENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
            + VL+A+D S  ++ AF  Y  + P  ++D IILLHS+  P         N+   LL+         E   +++++  + L    M QC+ R+L C++VS+ G P   IV A  ++ A++VV+G RG N I R ILGSVS YVL H   P ++V
Sbjct:   25 RRVLIAVDGSKGAQRAFD-YWSSIPGSQSDSIILLHSVEAP---------NVLTGLLLSPTFDYEEYEKQGRQLKQRSQQLSQELMAQCEKRRLHCKFVSEAGTPGATIVEAARKYAANVVVVGCRGNNGIRRTILGSVSDYVLQHSCRPVVVV 168
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A267DJS9 (Usp domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027842g3 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.523e-21
Identity = 63/150 (42.00%), Postives = 85/150 (56.67%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGD-VEIS-EDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKN---RKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKII 548
            K VLLA+D S + K AF  YLK   +  TD ++L+H++ PP   V +S  D  LK    +ME  +     E E LK  + D C     +K S  +V+  G+P + IV A ++  ADLVV+GSRGL  + R  LGSVS YVLHH   P +I
Sbjct:   21 KVVLLAVDSSPHCKRAFDMYLKQVRQ-PTDVLLLVHAVEPPSQPVALSVSDNFLKRHTELMEEQLA----EAERLKQRWKDACHQLGIKKFS--FVNVSGEPGEAIVQAAEEHEADLVVMGSRGLGTLRRTFLGSVSDYVLHHAYRPVLI 163
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A267GQM9 (Usp domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009186g1 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.793e-21
Identity = 63/150 (42.00%), Postives = 85/150 (56.67%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGD-VEIS-EDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKN---RKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKII 548
            K VLLA+D S + K AF  YLK   +  TD ++L+H++ PP   V +S  D  LK    +ME  +     E E LK  + D C     +K S  +V+  G+P + IV A ++  ADLVV+GSRGL  + R  LGSVS YVLHH   P +I
Sbjct:   22 KVVLLAVDSSPHCKRAFDMYLKQVRQ-PTDVLLLVHAVEPPSQPVALSVSDNFLKRHTELMEEQLA----EAERLKQRWKDACHQLGIKKFS--FVNVSGEPGEAIVQAAEEHEADLVVMGSRGLGTLRRTFLGSVSDYVLHHAYRPVLI 164
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO45800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRQ2])

HSP 1 Score: 91.6633 bits (226), Expect = 1.229e-23
Identity = 51/147 (34.69%), Postives = 84/147 (57.14%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPG-DVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
            + +LL ID S +S+ AF+WY+ N    + D++IL+H +        +S  G +  +    +N+M K  KE+++L+  +  + +   L  +   + GKP + I       + DL+++GSRGL  I R ILGSVS YVLHH ++P II+
Sbjct:   10 RRILLPIDSSKHSEDAFEWYVNNMHH-EEDELILVHVLDSAAIQTRVSSHGLVDDEF---KNEMNKGLKEVKALEEKYKTKAETASLKAKIEVRGGKPGETICQCSKDEHCDLILMGSRGLGSIRRTILGSVSDYVLHHAHVPTIII 152
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO34657 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNQ4])

HSP 1 Score: 85.5001 bits (210), Expect = 3.713e-21
Identity = 47/147 (31.97%), Postives = 81/147 (55.10%), Query Frame = 3 
Query:  120 VLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAP 560
            V++A+D S +S  AF +Y K S   K D+++L+H+     D    E  +    +  ++  +++  KE + L S F  +CK  K +C+  +K GKP + I   +++ NAD +V+G RG + + R ++GSVS Y + H   P  +V  P
Sbjct:   10 VVIAVDGSEHSDRAFDFYSK-SMHRKDDEVLLIHA-NDFADRHTQEHHHNVATVESLDRWLERCTKESKKLLSSFEKKCKENKFNCKLFTKIGKPGEVICEFMEEKNADQIVLGCRGQDTLRRTLMGSVSDYCIRHATKPVTVVPPP 154
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO44805 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT2])

HSP 1 Score: 83.9593 bits (206), Expect = 1.181e-20
Identity = 45/150 (30.00%), Postives = 77/150 (51.33%), Query Frame = 3 
Query:  111 SKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMEN---DMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
             + +++ +D S +S+ AF WY K +     D++ ++H+     D   +            E+    MKK   + +S+   +  +CK+ K+ C  ++K G P + I       NAD +++GSRGL  + R I+GSVS + LHH +IP  IV
Sbjct:    9 GRKIVIPVDGSKHSERAFDWY-KGALHRGNDEVFVVHAF----DPYAAPPTPYPYGFAFPEDWEQHMKKTVDDAKSVMEYYEKKCKDSKMKCTMLTKPGDPGETICEIAKDKNADQIIMGSRGLGTVRRTIVGSVSEFCLHHTHIPMSIV 153
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO34658 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNQ5])

HSP 1 Score: 80.4925 bits (197), Expect = 3.003e-19
Identity = 47/150 (31.33%), Postives = 80/150 (53.33%), Query Frame = 3 
Query:  120 VLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMEN-D--MKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAP 560
            V++A+D S +S  AF++Y +N  + K D+++L+H+     D+           L  +E  D  +++  +E + + S F  +CK  K +C+  +K G P + I    ++ NAD VV+G RG   + R  +GSVS Y +HH   P  +V  P
Sbjct:   10 VVIAVDGSEHSDRAFEFYSQNMHK-KGDEVLLIHA----NDIAERHIQLHPYGLATVEGWDKWLERCTEESKKMLSRFEKKCKENKFNCKLFTKVGNPGEVICDFTEEKNADQVVLGCRGQGTVRRTFMGSVSEYCIHHATTPITVVPPP 154
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO33143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST34])

HSP 1 Score: 79.7221 bits (195), Expect = 4.130e-19
Identity = 50/157 (31.85%), Postives = 90/157 (57.32%), Query Frame = 3 
Query:  111 SKTVLLAIDDSIYSKYAFQWY--LKNSPEVKTDQIILLHSMT-PPGDVEISEDGNLKVDLLVMEN---DMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQ-FNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAP 560
             + +L+ +D S +S+ AF WY  L +SP    D+++++H +  PP  +E              E     +K+ +++ E++   + D CK +KL  + +   GKPA +++  V +  +A+L+V+G+RG  +I R ILGSVS YV+HH ++P  ++ AP
Sbjct:    3 GRKILIPVDGSEHSERAFDWYAELLHSP---GDEVLVVHCIELPPVPLE----HQFPFVFAYYEEWSAMVKETREQHEAMLRSYEDICKEKKLHYEIMMVVGKPAGDVICQVARDVSANLIVLGTRGQGMIRRTILGSVSDYVVHHSHLPVAVIPAP 152
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000053435.1 (SMESG000053435.1)

HSP 1 Score: 314.309 bits (804), Expect = 6.996e-111
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 3 
Query:  105 SVSKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAPA 563
            SVSKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAPA
Sbjct:    5 SVSKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAPA 157
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000049288.1 (SMESG000049288.1)

HSP 1 Score: 314.309 bits (804), Expect = 6.996e-111
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 3 
Query:  105 SVSKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAPA 563
            SVSKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAPA
Sbjct:    5 SVSKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIVHAPA 157
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000053425.1 (SMESG000053425.1)

HSP 1 Score: 174.866 bits (442), Expect = 1.349e-55
Identity = 75/145 (51.72%), Postives = 110/145 (75.86%), Query Frame = 3 
Query:  117 TVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
             VL+A+D S  +  AF WY+ NSPEVK DQIIL H+++PP ++ I++DG+L  D+   E +++ +Q E   ++S FM++C+  +L C+WV++WGKP  +I+ AVD+F ADLVV+GSRGLN + R ILGSVSTYVLHH+++P +IV
Sbjct:   18 NVLVALDGSKNATKAFDWYINNSPEVKNDQIILFHNLSPPSNISITDDGDLDFDISDFEKEIQNVQAEATKMESFFMNECQRNQLKCKWVNQWGKPNHSILKAVDKFQADLVVMGSRGLNALSRTILGSVSTYVLHHIHVPLLIV 162
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000075112.1 (SMESG000075112.1)

HSP 1 Score: 124.02 bits (310), Expect = 5.981e-36
Identity = 63/150 (42.00%), Postives = 93/150 (62.00%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGDVEISEDGNLKVDLLVMEND----MKKIQKEMESLKSVFMDQCKNRKLSCQWVSKWGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
            +TV++ ID S YS  AF+W+ K+  +  TD+IIL HS+ P   V       L  +    + D    +KK+Q E + L++ FMD+C+   L C W +  G P  N+VHAV++  A +VV+G+RGLNL  R ++GSVSTYV  HVN+P ++V
Sbjct:    6 RTVVIGIDGSKYSDDAFKWFKKHYAQPATDEIILFHSICPSASVSAPILKGLPTN---PDKDYAIQLKKLQLEADVLEAKFMDRCEKSGLKCTWKTSLGSPNLNLVHAVEENKAQMVVMGTRGLNLFRRTLVGSVSTYVTQHVNVPVLVV 152
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000051705.1 (SMESG000051705.1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.497e-19
Identity = 57/154 (37.01%), Postives = 82/154 (53.25%), Query Frame = 3 
Query:  114 KTVLLAIDDSIYSKYAFQWYLKN--SPEVKTDQIILLHSMTPP--GDVEISEDGNLKVDLLVMENDMKKIQKEMES---LKSVFMDQCKNRKLSCQWVSKWGK-PAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPKIIV 551
            K +LL ID S YS+ AF W +KN   P+   D +I +H + PP      +    N+ VD L      KKIQK +E    L S + ++C N     ++ +   K P + I  +    NAD++VIG+RGL    R +LGSVS YV++  +IP  IV
Sbjct:    5 KRILLPIDGSYYSQKAFHWAVKNLYKPD---DVLIFVHVVNPPVLKSFTLKSGINIPVDEL-----SKKIQKRIEKSEKLHSSYSNECLNYNAKHEYYTITHKSPGEGICQSSVDNNADIIVIGNRGLGKFKRTLLGSVSDYVINRSSIPVTIV 150
The following BLAST results are available for this feature:
BLAST of Usp domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|P74148|Y1388_SYNY31.219e-1033.33Universal stress protein Sll1388 OS=Synechocystis ... [more]
sp|Q8LGG8|USPAL_ARATH9.514e-1027.86Universal stress protein A-like protein OS=Arabido... [more]
sp|P72745|Y1101_SYNY31.693e-627.84Universal stress protein Slr1101 OS=Synechocystis ... [more]
sp|A0QZA1|Y3950_MYCS25.695e-626.35Universal stress protein MSMEG_3950/MSMEI_3859 OS=... [more]
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BLAST of Usp domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8GWE61.011e-2338.26Usp domain-containing protein OS=Macrostomum ligna... [more]
A0A267H1T18.853e-2337.58Usp domain-containing protein OS=Macrostomum ligna... [more]
A0A1I8IW268.433e-2236.36Usp domain-containing protein OS=Macrostomum ligna... [more]
A0A267DJS91.523e-2142.00Usp domain-containing protein (Fragment) OS=Macros... [more]
A0A267GQM91.793e-2142.00Usp domain-containing protein (Fragment) OS=Macros... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO458001.229e-2334.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO346573.713e-2131.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448051.181e-2030.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO346583.003e-1931.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO331434.130e-1931.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000053435.16.996e-111100.00SMESG000053435.1[more]
SMESG000049288.16.996e-111100.00SMESG000049288.1[more]
SMESG000053425.11.349e-5551.72SMESG000053425.1[more]
SMESG000075112.15.981e-3642.00SMESG000075112.1[more]
SMESG000051705.13.497e-1937.01SMESG000051705.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020630 ID=SMED30020630|Name=Usp domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=630bp
CGATCTGATTTACCTTGAATCGTTTATTCATTTTCATTTTTATTTTTTTA
TCATTTTTTTATATAAATTAAGATAATTTTTTTTGATTTGGCATGGGAGG
AAATTCCGTATCTAAAACAGTCCTTCTGGCGATTGACGACAGCATTTATT
CAAAATACGCGTTTCAATGGTACCTAAAAAACAGTCCAGAGGTGAAAACT
GATCAAATAATACTACTTCATTCCATGACACCTCCTGGGGACGTTGAAAT
CTCCGAAGACGGAAATTTAAAAGTAGATTTACTTGTGATGGAAAACGATA
TGAAAAAAATTCAAAAAGAAATGGAATCGCTAAAATCTGTGTTCATGGAT
CAGTGCAAGAATCGCAAACTATCCTGTCAATGGGTAAGTAAATGGGGCAA
GCCAGCGCAGAATATTGTCCATGCTGTTGATCAATTTAATGCTGATCTTG
TAGTAATAGGTTCTAGGGGGCTCAATTTGATCGATCGAGCGATCCTTGGA
TCAGTTTCAACATATGTTCTCCATCATGTCAACATTCCCAAAATTATTGT
TCATGCACCAGCATAATGAAAAACATTTTAACTATTTTATTCTTTTTATT
TTCGTTTTTCTAATAAACAATTATTCTATT
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protein sequence of SMED30020630-orf-1

>SMED30020630-orf-1 ID=SMED30020630-orf-1|Name=SMED30020630-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=158bp
MGGNSVSKTVLLAIDDSIYSKYAFQWYLKNSPEVKTDQIILLHSMTPPGD
VEISEDGNLKVDLLVMENDMKKIQKEMESLKSVFMDQCKNRKLSCQWVSK
WGKPAQNIVHAVDQFNADLVVIGSRGLNLIDRAILGSVSTYVLHHVNIPK
IIVHAPA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000035Category 3 cell
Vocabulary: INTERPRO
TermDefinition
IPR006015Universal_stress_UspA
IPR006016UspA
IPR014729Rossmann-like_a/b/a_fold
Vocabulary: biological process
TermDefinition
GO:0006950response to stress
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 63..83
NoneNo IPR availablePANTHERPTHR31964:SF113ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEINcoord: 8..154
NoneNo IPR availablePANTHERPTHR31964FAMILY NOT NAMEDcoord: 8..154
NoneNo IPR availableCDDcd00293USP_Likecoord: 9..153
e-value: 1.91757E-24
score: 89.7366
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 8..155
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 114..126
score: 48.0
coord: 7..25
score: 29.05
coord: 132..154
score: 35.65
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 4..157
e-value: 5.2E-33
score: 116.7
IPR006016UspAPFAMPF00582Uspcoord: 8..154
e-value: 1.2E-23
score: 84.2