Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Overview
NameAlpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Smed IDSMED30020599
Length (bp)1612
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (SMED30020599) t-SNE clustered cells

Violin plots show distribution of expression levels for Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (SMED30020599) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (SMED30020599) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (SMED30020599) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020599

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30020599SMESG000047050.1 dd_Smed_v4_9232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30020599SMESG000047050.1 dd_Smed_v4_9232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020599SMESG000047050.1 dd_Smed_v4_9232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020599SMESG000047050.1 dd_Smed_v4_9232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30020599SMESG000047050.1 dd_Smed_v4_9232_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Human
Match: MGAT2 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Source:HGNC Symbol;Acc:HGNC:7045])

HSP 1 Score: 308.916 bits (790), Expect = 2.543e-99
Identity = 144/350 (41.14%), Postives = 222/350 (63.43%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLD---LFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF-NPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEM-IYMGATTTRSKEFHPNGGWSDLRDRNLCK 1426
            L++Q+N +Q + N+D    +   E  +V+Q+HNR EYL +L++SL   + I   L+IFSHD +  E N++I  + F P++Q+FFPF+ Q++ +EFPG + +DC  D+ K  +    C NA + DS+G+YR  K++QTKHHW WK HFV++R++ L  +   I+F+EEDHYL  DF HV + M   ++++ P+  ++ LGTY    SF   + K+ +  W S+ HNMG+   R+ + K+ +C   FC +DDYNWDW+LQ++   CL + ++V L+   PRIFH G+CG+HHK K C P     +I+ LL+ N+QY++P+ + I    T         NGGW D+RD  LCK
Sbjct:   94 LVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKV-LVPQIPRIFHAGDCGMHHK-KTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCK 441
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Celegans
Match: gly-20 (GLYcosylation related [Source:UniProtKB/TrEMBL;Acc:O17560])

HSP 1 Score: 280.026 bits (715), Expect = 4.586e-88
Identity = 167/460 (36.30%), Postives = 244/460 (53.04%), Query Frame = 2 
Query:  143 NNYLGVNRDVKELKTDQINLRLSLIDKLRTQFIEIFNFNPSPNEGPVLLERKRNRNFKQNNTYPISSIFNRNIELMNASNLLRLLHQINSEQIILNLDLFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNP-IVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGA--WLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQLLDNLTSLESSNGHLSVLESLL 1513
            N++ G+N++V +L  ++ N RLS+              N  P+      + K + + K +      S  N N +++NA+        +++ + IL            VIQ+H+R  YL  LI S+ N K I  +LL+FSHD+     N++IR+I FA + QIF+P+N Q+F + FPG +  DC   MK+  +Q  NC N +  D YGNYR  + TQ KHHW WK +FVFD +      ++P ++ +EEDH L  D LHVL +++S R K      II LG Y K  +        +G   W SS HNMGM   ++ W KIK C + FC++DDYNWDWSL  +  +CL + F+V+  K SPR+ HIG+CG+H     C  +  L   Q L  Q++  L+P  +     +    K    NGGW D+RDR LC++  N S L      +  S+   SVL  LL
Sbjct:   52 NDWNGLNKEVVDLLKNESN-RLSI--------------NEKPDLSGWNFKTKEDMSLKGSEIVESVSFLNENFDILNAAKF----GDLSTVKTIL------------VIQVHDRPVYLQYLIESMRNTKGIEDTLLVFSHDINVGIINEMIRNITFARVYQIFYPYNLQLFPTVFPGQSPSDCPEKMKRDKAQETNCSNWSSPDKYGNYRVAQLTQIKHHWWWKMNFVFDGIVEKYSMKDPWVLLLEEDHMLAPDALHVLDIIVSNRPKYCENCEIISLGFYLKSTNKYGQDIAHLGVHPWYSSKHNMGMALQKNTWQKIKGCSEMFCKWDDYNWDWSLMQISAKCLPQRFRVIFTK-SPRVIHIGDCGVHTHR--CEAHKALQSTQELFRQHKDLLFPTSLSVTDTSRRSLKPSKENGGWGDIRDRQLCEI--NKSPL------VRVSSQSASVLHKLL 469
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Fly
Match: Mgat2 (gene:FBgn0039738 transcript:FBtr0100418)

HSP 1 Score: 323.939 bits (829), Expect = 6.363e-105
Identity = 154/358 (43.02%), Postives = 230/358 (64.25%), Query Frame = 2 
Query:  371 NASNLLRLLHQINSEQIILNLDLFP---NPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHS-FNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLL--SQNRQYLYPDEM-IYMGATTTRSKEFHPNGGWSDLRDRNLC 1423
            N + + R + + N  Q++LN D+F    N    IV+Q+H R+ YL  LI SL   +DI K LL+FSHD Y  + N +++ I F  ++QIF+P++ Q   +E+PG +  DC  ++KK+ + + NC NA + D YG+YR  K+TQTKHHW WKA+ VF+ L         ++F+EEDHY+ EDFL++L +M    K   PQ +++ LGTY K  + +   SK+++  W+SS HNMG  F+R+ W+ I+KC + FC +DDYNWDWSLQHV ++CL+ +   M++K  PR+FHIGECG+HHKNK C    V+ K+Q +L  ++N   L+P  + + + +   +SK    NGGW D+RD  LC
Sbjct:  124 NITEIKRQIVRYNDMQMVLNEDVFGPLQNDSVIIVVQVHTRITYLRHLIVSLAQARDISKVLLVFSHDYYDDDINDLVQQIDFCKVMQIFYPYSIQTHPNEYPGVDPNDCPRNIKKEQALITNCNNAMYPDLYGHYREAKFTQTKHHWIWKANRVFNELEVTRYHTGLVLFLEEDHYVAEDFLYLLAMMQQRTKDLCPQCNVLSLGTYLKTFNYYTYHSKVEVMPWVSSKHNMGFAFNRTTWSNIRKCARHFCTYDDYNWDWSLQHVSQQCLRRKLHAMIVK-GPRVFHIGECGVHHKNKNCESNQVISKVQHVLRIARNSHQLFPRSLTLTVPSLMKKSKLRKGNGGWGDMRDHELC 480
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Fly
Match: Mgat2 (gene:FBgn0039738 transcript:FBtr0334705)

HSP 1 Score: 316.62 bits (810), Expect = 8.216e-102
Identity = 155/379 (40.90%), Postives = 231/379 (60.95%), Query Frame = 2 
Query:  371 NASNLLRLLHQINSEQIILNLDLFP---NPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTY----------EKVHS------------FNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLL--SQNRQYLYPDEM-IYMGATTTRSKEFHPNGGWSDLRDRNLC 1423
            N + + R + + N  Q++LN D+F    N    IV+Q+H R+ YL  LI SL   +DI K LL+FSHD Y  + N +++ I F  ++QIF+P++ Q   +E+PG +  DC  ++KK+ + + NC NA + D YG+YR  K+TQTKHHW WKA+ VF+ L         ++F+EEDHY+ EDFL++L +M    K   PQ +++ LGTY           K+H+             N   K+++  W+SS HNMG  F+R+ W+ I+KC + FC +DDYNWDWSLQHV ++CL+ +   M++K  PR+FHIGECG+HHKNK C    V+ K+Q +L  ++N   L+P  + + + +   +SK    NGGW D+RD  LC
Sbjct:  124 NITEIKRQIVRYNDMQMVLNEDVFGPLQNDSVIIVVQVHTRITYLRHLIVSLAQARDISKVLLVFSHDYYDDDINDLVQQIDFCKVMQIFYPYSIQTHPNEYPGVDPNDCPRNIKKEQALITNCNNAMYPDLYGHYREAKFTQTKHHWIWKANRVFNELEVTRYHTGLVLFLEEDHYVAEDFLYLLAMMQQRTKDLCPQCNVLSLGTYLKTFNYYTYHSKLHARVRTAKIDRQSIVNDYRKVEVMPWVSSKHNMGFAFNRTTWSNIRKCARHFCTYDDYNWDWSLQHVSQQCLRRKLHAMIVK-GPRVFHIGECGVHHKNKNCESNQVISKVQHVLRIARNSHQLFPRSLTLTVPSLMKKSKLRKGNGGWGDMRDHELC 501
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Fly
Match: Mgat2 (gene:FBgn0039738 transcript:FBtr0085602)

HSP 1 Score: 180.259 bits (456), Expect = 2.804e-49
Identity = 88/214 (41.12%), Postives = 134/214 (62.62%), Query Frame = 2 
Query:  371 NASNLLRLLHQINSEQIILNLDLFP---NPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF 1003
            N + + R + + N  Q++LN D+F    N    IV+Q+H R+ YL  LI SL   +DI K LL+FSHD Y  + N +++ I F  ++QIF+P++ Q   +E+PG +  DC  ++KK+ + + NC NA + D YG+YR  K+TQTKHHW WKA+ VF+ L         ++F+EEDHY+ EDFL++L +M    K   PQ +++ LGTY K  ++
Sbjct:  124 NITEIKRQIVRYNDMQMVLNEDVFGPLQNDSVIIVVQVHTRITYLRHLIVSLAQARDISKVLLVFSHDYYDDDINDLVQQIDFCKVMQIFYPYSIQTHPNEYPGVDPNDCPRNIKKEQALITNCNNAMYPDLYGHYREAKFTQTKHHWIWKANRVFNELEVTRYHTGLVLFLEEDHYVAEDFLYLLAMMQQRTKDLCPQCNVLSLGTYLKTFNY 337

HSP 2 Score: 154.836 bits (390), Expect = 2.958e-40
Identity = 65/139 (46.76%), Postives = 95/139 (68.35%), Query Frame = 2 
Query: 1016 KIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLL--SQNRQYLYPDEM-IYMGATTTRSKEFHPNGGWSDLRDRNLC 1423
            K+++  W+SS HNMG  F+R+ W+ I+KC + FC +DDYNWDWSLQHV ++CL+ +   M++K  PR+FHIGECG+HHKNK C    V+ K+Q +L  ++N   L+P  + + + +   +SK    NGGW D+RD  LC
Sbjct:  463 KVEVMPWVSSKHNMGFAFNRTTWSNIRKCARHFCTYDDYNWDWSLQHVSQQCLRRKLHAMIVK-GPRVFHIGECGVHHKNKNCESNQVISKVQHVLRIARNSHQLFPRSLTLTVPSLMKKSKLRKGNGGWGDMRDHELC 600
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Zebrafish
Match: mgat2 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Source:ZFIN;Acc:ZDB-GENE-070410-54])

HSP 1 Score: 320.472 bits (820), Expect = 7.237e-104
Identity = 156/379 (41.16%), Postives = 232/379 (61.21%), Query Frame = 2 
Query:  314 KQNNTYPISSIFNRNIELMNASNLLRLLHQINSEQIILNLDLF----PNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIY---MGATTTRSKEFH-PNGGWSDLRDRNLCK 1426
            KQ    P++     N  L+       ++ Q+N +Q + N + F       +  IV+Q+HNR EYL +L++SL   K I   LLIFSHD + PE N+I+ S+ F  ++QIFFPF+ Q++  EFPG + +DC  D+ KK +    C NA + DS+G+YR  K++QTKHHW WK HFV+DR+R L+  +  ++ IEEDHYL  DF H+L+LM S +K++ P   I+ LG+Y  +   + ++K+++ AW S+ HNMGM  +R  + K+ +C   FC +DDYNWDWSLQH+   CL    +VM +  +PRIFH G+CG+HHK   C P     KI+ +L  +   L+P +++    + A+  +    H  NGGW D+RD  LCK
Sbjct:   71 KQQQQQPVAKSEADNTTLVYRG----IVFQLNFDQNLKNEEKFRAVRQKDDLVIVVQVHNRPEYLRLLVDSLRKSKGIENILLIFSHDFWSPEINQIVASVDFCLVLQIFFPFSIQLYPQEFPGNDPRDCPRDIPKKEALTLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWDRVRVLKDHKGLVLLIEEDHYLAPDFYHLLKLMASLKKEQCPDCDILSLGSYGHIGYSSKANKVEVKAWKSTEHNMGMALNRDAYQKLLRCTDAFCTYDDYNWDWSLQHLTVTCLPAFLKVM-VSEAPRIFHAGDCGMHHKKSACMPSGQKTKIENVLQNSGNQLFPKQLLITKRLPASGAKGVAPHVKNGGWGDIRDHELCK 444
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Zebrafish
Match: si:ch73-91k6.2 (si:ch73-91k6.2 [Source:ZFIN;Acc:ZDB-GENE-050208-270])

HSP 1 Score: 285.034 bits (728), Expect = 1.131e-90
Identity = 141/378 (37.30%), Postives = 211/378 (55.82%), Query Frame = 2 
Query:  296 KRNRNFKQNNTYPISSIFNRNIELMNASNLLRLLHQINSEQIILNLDLFP-NPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNP-SSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLC 1423
            K + N ++ +     +      +     N+ + ++  N  Q I N D FP +PE  IV+Q+HNR  YL +LI SL  V  I  +L+IFSHD +  E + +++ I F  ++QIFFP++ Q++ +EFPG + +DC  D+ K ++  K C NA   DSYG+YR    TQTKHHW WK HFVF+R+R L  +   +VFIEED+YL  DF   L+ M   RK       ++ LG +  +  F+  SSK +   WLS+ HN+GM   R ++ K+  C   FC +DDYNWDW+LQ++   C+ +  +V++   S R+ H G+CG+H K   C P +   K+   L   +  L+P  +          +    NGGW D+RD  LC
Sbjct:   31 KDDSNMEETDKESTHTAVMVKFDYGTIQNMRKSIYDSNYRQFIQNGDQFPLDPEVVIVVQVHNRPAYLKMLIQSLEKVTGIQNALVIFSHDYFSEEISGMVKEIAFCRVLQIFFPYSIQLYPNEFPGQDPQDCPRDISKDDAIKKGCLNAKHPDSYGHYREASITQTKHHWWWKLHFVFERVRILRGYNGYVVFIEEDNYLLPDFHEYLKSMSELRKTSCGDCDVLALGNHNSLSGFHELSSKTETSGWLSTKHNIGMGITRELYYKLMGCNNAFCTYDDYNWDWTLQNLSGTCISKPIKVLVALGS-RVIHTGDCGLHQKTD-CRPEMAQEKVDSALKDVKSILFPPILTLTDNGPVVHQPHMKNGGWGDIRDHTLC 406
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Xenopus
Match: mgat2 (mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [Source:NCBI gene;Acc:448439])

HSP 1 Score: 299.671 bits (766), Expect = 6.042e-96
Identity = 145/348 (41.67%), Postives = 224/348 (64.37%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLDLF--PNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFH-PNGGWSDLRDRNLCK 1426
            ++ Q N +Q I N++    P  +  +V+Q+HNR E+L  L++SL   K I   LL+FSHD + PE N+II S+ F  ++QIFFPF+ Q++ +EFPG++ KDC  D+KKK++    C NA + DS+G+YR  K++QTKHHW WK  FV+D+L+ L+ +   ++FIEEDHYL  DF ++L+ M  ++ ++     ++CLGTY +    + + K+++  W S+ HNMGM   R  + K+  C + FC +DDYNWDW+LQ++   CL + ++VM +   PRI+HIG+CG+HH NK C P     K++ L + ++Q L+P+ +      T  +   H  NGGW D+RD  LCK
Sbjct:   81 VVFQWNFDQAIRNVEKINRPQDDVVVVVQVHNRPEFLKRLLDSLGKAKGIENVLLVFSHDYWSPEINQIIASVDFCQVLQIFFPFSIQLYPNEFPGHDPKDCPRDIKKKDAIELGCINAEYPDSFGHYREAKFSQTKHHWWWKLQFVWDKLKVLKEYHGLVLFIEEDHYLAPDFYYILKKMWQKKNEECSDCDMLCLGTYAQTPFADKAGKVEVKTWRSTEHNMGMAMTRETYKKLISCSETFCTYDDYNWDWTLQYLTVNCLPKFWKVM-VPEVPRIYHIGDCGMHH-NKPCRPTTESAKLEALFTSSQQELFPENIAISRRYTMAALSPHVKNGGWGDIRDHELCK 426
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Xenopus
Match: ENSXETT00000014814.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase-like [Source:NCBI gene;Acc:100493854])

HSP 1 Score: 191.43 bits (485), Expect = 9.184e-55
Identity = 119/398 (29.90%), Postives = 190/398 (47.74%), Query Frame = 2 
Query:  284 LLERKRNRNFKQNNTYPISSIFNRNIELMNASNL-LRL-LHQINSEQIILNLDLFPN-PEFAIVIQIHN---RLEYLTILINSL--INVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLK-----NSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQY---LYPDEMIYMG-ATTTRSKEFHPNGGWSDLRDRNLCK 1426
            L  R+   NF+     P      R   L     L +RL  ++ N  Q +LN D FP+ PE  +V+Q          L +L  SL     +   + LL+ S +    E    + SI F  ++ ++FP++   +  EFPG +  DC  D+ K+++  + C NA + DS+G+YR   +   KHHW WK HF ++R+R +  +   +VF+EE  YL  D+ H+LRLM  + +++  Q  ++ LG              ++  +++  H   +   R ++ ++  C+ EFC +DDYNWDWSLQH+   CL    +V+  +     N P +   GECG   ++  C         Q L  Q RQ    L+P  +         R+     NGGW D+RD  LC+
Sbjct:   26 LSTRQNEGNFQLTTPGPSEGNNPRGFRLPAGPPLQMRLGAYRNNLRQPVLNADRFPSRPELVVVVQFRGGPGSGARLRLLAESLRAAGPRANGRLLLVLSMEKPCREVADAMLSIDFCRVLPLYFPYSLSFYPEEFPGADPNDCPRDLSKESALQRRCNNAEFPDSHGHYREAPFALDKHHWWWKLHFTWERVREVSGYGGYVVFLEEGSYLLPDWQHMLRLMQKQCQEEGCQ--LLSLGGTASPDPSPDPQHTEVSGFVAPKHRSAVGMPRPLYYQLMGCLTEFCTYDDYNWDWSLQHLSASCLSHPLKVLSARLPRVLNLPSVPKEGECG---RSGACP--STDAASQNLREQVRQLSGRLFPKTITVASRQQEVRNPPQTKNGGWGDIRDHALCQ 416
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Mouse
Match: Mgat2 (mannoside acetylglucosaminyltransferase 2 [Source:MGI Symbol;Acc:MGI:2384966])

HSP 1 Score: 315.849 bits (808), Expect = 3.609e-102
Identity = 147/354 (41.53%), Postives = 227/354 (64.12%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLD---LFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF-NPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIY-----MGATTTRSKEFHPNGGWSDLRDRNLCK 1426
            L++Q+N +Q++ N+     +   E  +V+Q+HNR EYL +LI+SL   + I + L+IFSHD +  E N +I  + F P++Q+FFPF+ Q++ +EFPG + +DC  D+KK  +    C NA + DS+G+YR  K++QTKHHW WK HFV++R++ L+ +   I+F+EEDHYL  DF HV + M   ++++ P   ++ LGTY  + SF   + K+ +  W S+ HNMG+   R  + K+ +C   FC +DDYNWDW+LQ++   CL + ++V L+  +PRIFH G+CG+HHK K C P     +I+ LL+ N+QYL+P+ ++      M A +   K    NGGW D+RD  LCK
Sbjct:   89 LVYQLNFDQMLRNVGNDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIQEVLVIFSHDFWSAEINSLISRVDFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPGCDVLSLGTYTTIRSFYGIADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCTYDDYNWDWTLQYLTLACLPKIWKV-LVPQAPRIFHAGDCGMHHK-KTCRPSTQSAQIESLLNSNKQYLFPETLVIGEKFPMAAISPPRK----NGGWGDIRDHELCK 436
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. UniProt/SwissProt
Match: sp|Q09326|MGAT2_RAT (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus norvegicus OX=10116 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 1.642e-103
Identity = 150/354 (42.37%), Postives = 229/354 (64.69%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLD---LFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF-NPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIY-----MGATTTRSKEFHPNGGWSDLRDRNLCK 1426
            L++Q+N +Q++ N+D    +   E  +V+Q+HNR EYL +LI+SL   + I + L+IFSHD +  E N +I S+ F P++Q+FFPF+ Q++ SEFPG + +DC  D+KK  +    C NA + DS+G+YR  K++QTKHHW WK HFV++R++ L+ +   I+F+EEDHYL  DF HV + M   ++++ P   ++ LGTY  + SF   + K+ +  W S+ HNMG+   R  + K+ +C   FC +DDYNWDW+LQ++   CL + ++V L+  +PRIFH G+CG+HHK K C P     +I+ LL+ N+QYL+P+ ++      M A +   K    NGGW D+RD  LCK
Sbjct:   89 LVYQLNFDQMLRNVDKDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIREVLVIFSHDFWSAEINSLISSVDFCPVLQVFFPFSIQLYPSEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPGCDVLSLGTYTTIRSFYGIADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCTYDDYNWDWTLQYLTLACLPKVWKV-LVPQAPRIFHAGDCGMHHK-KTCRPSTQSAQIESLLNNNKQYLFPETLVIGEKFPMAAISPPRK----NGGWGDIRDHELCK 436
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. UniProt/SwissProt
Match: sp|Q921V5|MGAT2_MOUSE (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus musculus OX=10090 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 2.525e-101
Identity = 147/354 (41.53%), Postives = 227/354 (64.12%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLD---LFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF-NPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIY-----MGATTTRSKEFHPNGGWSDLRDRNLCK 1426
            L++Q+N +Q++ N+     +   E  +V+Q+HNR EYL +LI+SL   + I + L+IFSHD +  E N +I  + F P++Q+FFPF+ Q++ +EFPG + +DC  D+KK  +    C NA + DS+G+YR  K++QTKHHW WK HFV++R++ L+ +   I+F+EEDHYL  DF HV + M   ++++ P   ++ LGTY  + SF   + K+ +  W S+ HNMG+   R  + K+ +C   FC +DDYNWDW+LQ++   CL + ++V L+  +PRIFH G+CG+HHK K C P     +I+ LL+ N+QYL+P+ ++      M A +   K    NGGW D+RD  LCK
Sbjct:   89 LVYQLNFDQMLRNVGNDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIQEVLVIFSHDFWSAEINSLISRVDFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPGCDVLSLGTYTTIRSFYGIADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCTYDDYNWDWTLQYLTLACLPKIWKV-LVPQAPRIFHAGDCGMHHK-KTCRPSTQSAQIESLLNSNKQYLFPETLVIGEKFPMAAISPPRK----NGGWGDIRDHELCK 436
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. UniProt/SwissProt
Match: sp|O19071|MGAT2_PIG (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus scrofa OX=9823 GN=MGAT2 PE=3 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 2.945e-101
Identity = 143/349 (40.97%), Postives = 224/349 (64.18%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLDLFPN---PEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF-NPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCK 1426
            L++Q+N +Q + N+D   +    E  +V+Q+HNR EYL +L++SL   + I   L+IFSHD +  E N++I  + F P++Q+FFPF+ Q++ +EFPG + +DC  D++K  +    C NA + DS+G+YR  K++QTKHHW WK HFV++R++ L  +   I+F+EEDHY+  DF HV + M + ++++ P+  ++ LGTY  V SF + + K+ +  W S+ HNMG+   R  + K+ +C   FC +DDYNWDW+LQ++   CL + ++V L+   PRIFH G+CG+HHK K C P     +I+ LL+ N+QY++P+ +      T        NGGW D+RD  LCK
Sbjct:   94 LVYQLNFDQTLRNVDKVSSWVPRELVLVVQVHNRAEYLKLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVDFCPVLQVFFPFSIQLYPNEFPGTDPRDCPRDLEKNAALKMGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKVLRDYAGLILFLEEDHYVAPDFYHVFKKMWNLKQQECPECDVLSLGTYTTVRSFRDVADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKV-LVPQVPRIFHAGDCGMHHK-KTCRPSTQSAQIESLLNSNKQYMFPETLTISEKLTAALSPPRKNGGWGDIRDHELCK 440
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. UniProt/SwissProt
Match: sp|Q10469|MGAT2_HUMAN (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo sapiens OX=9606 GN=MGAT2 PE=1 SV=1)

HSP 1 Score: 308.916 bits (790), Expect = 1.221e-98
Identity = 144/350 (41.14%), Postives = 222/350 (63.43%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLD---LFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF-NPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEM-IYMGATTTRSKEFHPNGGWSDLRDRNLCK 1426
            L++Q+N +Q + N+D    +   E  +V+Q+HNR EYL +L++SL   + I   L+IFSHD +  E N++I  + F P++Q+FFPF+ Q++ +EFPG + +DC  D+ K  +    C NA + DS+G+YR  K++QTKHHW WK HFV++R++ L  +   I+F+EEDHYL  DF HV + M   ++++ P+  ++ LGTY    SF   + K+ +  W S+ HNMG+   R+ + K+ +C   FC +DDYNWDW+LQ++   CL + ++V L+   PRIFH G+CG+HHK K C P     +I+ LL+ N+QY++P+ + I    T         NGGW D+RD  LCK
Sbjct:   94 LVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKV-LVPQIPRIFHAGDCGMHHK-KTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCK 441
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. UniProt/SwissProt
Match: sp|Q9FT88|MGAT2_ARATH (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabidopsis thaliana OX=3702 GN=GNT2 PE=1 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 3.139e-46
Identity = 114/354 (32.20%), Postives = 167/354 (47.18%), Query Frame = 2 
Query:  428 NLDLFPN---PEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSF-----HNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRI--FHIGECGIHHK---------NKVCNPYVVLYKIQRLLSQNRQY---LYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLC 1423
            N DLFP+       IV+ +HNR +Y  + + SL  VK I ++LLI SHD YF E N+I+ SI+F  + QIF P++  I+++ FPG    DC    K K  + K     N  D YGN+R+ K    KHHW W  + V+D L   +  +  I+FIEEDH+L  +    ++ +   +  K P     C        +  PS     G  L S       N+G +F+RSVW  I +  +EFC FDDYNWD ++              +     PR    H G+CG+H           N V N  + + +  ++++    +   +Y  +  Y          F   GGW D RDR+LC
Sbjct:   87 NTDLFPDLAKDRVVIVLYVHNRAQYFRVTVESLSKVKGISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTLNDC----KNKGDEAKGHCEGN-PDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFIEEDHFLFPNAYRNIQTLTRLKPAKCPD----CFAA-----NLAPSDVKSRGEGLESLVAERMGNVGYSFNRSVWENIHQKAREFCFFDDYNWDITMWATVFPSFGSPVYTL---RGPRTSAVHFGKCGLHQGRGDEGDCIDNGVVN--IEVKETDKVVNIKEGWGVRVYKHQAGYKAG-------FEGWGGWGDDRDRHLC 414
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. TrEMBL
Match: A0A267FKN7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004091g1 PE=4 SV=1)

HSP 1 Score: 386.341 bits (991), Expect = 2.239e-125
Identity = 174/361 (48.20%), Postives = 243/361 (67.31%), Query Frame = 2 
Query:  371 NASNLLRLLHQINSEQIILNLDLFPNP-EFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNP-SSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQ 1447
            NA+ +L  +  IN+E  ILN   +  P    IV+Q+H+R+ YL +LI+SL  VKD+   LL+FSHD+Y    N ++RSIRFA ++QIF+P+ +QI+   FPG + +DC  D     ++   C NA W D YG+YR  KYTQTKHHW +K + +F+R   L  F    VF+EEDHY+ ED LH+LRLM++ RK     V I+ LGTYEK   F   ++++ +  W+SSFHNMGM+F R++W +IK C+  FC FDDYNWDWSL H+  +C K  FQ M+++ SPR+FHIG+CG+HHKNKVCNP ++  K+ R+L+ N   LYPD++++      R++   PNGGW DLRDR LC   FN+S+
Sbjct:  140 NATAILDAMLAINAEHEILNAQFYNGPITEVIVVQVHDRIGYLYLLIDSLRRVKDVEDCLLVFSHDVYSSAINALVRSIRFARVLQIFYPYTKQIYFDRFPGNDPRDCPRDAGLYRARQLGCNNAEWPDKYGHYRESKYTQTKHHWLYKMNRLFERSPVLANFTGQFVFLEEDHYVVEDLLHMLRLMVAARKDLSQNVEILTLGTYEKKPDFTRNTAQVVVDKWVSSFHNMGMSFSRAIWDQIKPCLPAFCSFDDYNWDWSLNHILTKCTKRPFQTMVIRISPRVFHIGQCGVHHKNKVCNPALLSSKLDRMLANNIAKLYPDQLMFNAKLKIRARALKPNGGWGDLRDRRLCSSFFNSSE 500
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. TrEMBL
Match: A0A1I8JGE4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 385.956 bits (990), Expect = 2.966e-125
Identity = 174/361 (48.20%), Postives = 243/361 (67.31%), Query Frame = 2 
Query:  371 NASNLLRLLHQINSEQIILNLDLFPNP-EFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNP-SSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQ 1447
            NA+ +L  +  IN+E  ILN   +  P    IV+Q+H+R+ YL +LI+SL  VKD+   LL+FSHD+Y    N ++RSIRFA ++QIF+P+ +QI+   FPG + +DC  D     ++   C NA W D YG+YR  KYTQTKHHW +K + +F+R   L  F    VF+EEDHY+ ED LH+LRLM++ RK     V I+ LGTYEK   F   ++++ +  W+SSFHNMGM+F R++W +IK C+  FC FDDYNWDWSL H+  +C K  FQ M+++ SPR+FHIG+CG+HHKNKVCNP ++  K+ R+L+ N   LYPD++++      R++   PNGGW DLRDR LC   FN+S+
Sbjct:  140 NATAILDAMLAINAEHEILNAQFYNGPITEVIVVQVHDRIGYLYLLIDSLRRVKDVEDCLLVFSHDVYSSAINALVRSIRFARVLQIFYPYTKQIYFDRFPGNDPRDCPRDAGLYRARQLGCNNAEWPDKYGHYRESKYTQTKHHWLYKMNRLFERSPVLANFTGQFVFLEEDHYVVEDLLHMLRLMVAARKDLSQNVEILTLGTYEKKPDFTRNTAQVVVDKWVSSFHNMGMSFSRAIWDQIKPCLPAFCSFDDYNWDWSLNHILTKCTKRPFQTMVIRISPRVFHIGQCGVHHKNKVCNPALLSSKLDRMLANNIAKLYPDQLMFNAKLKIRARALKPNGGWGDLRDRRLCSSFFNSSE 500
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. TrEMBL
Match: A0A267FTV0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004091g2 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 9.330e-125
Identity = 174/361 (48.20%), Postives = 243/361 (67.31%), Query Frame = 2 
Query:  371 NASNLLRLLHQINSEQIILNLDLFPNP-EFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNP-SSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQ 1447
            NA+ +L  +  IN+E  ILN   +  P    IV+Q+H+R+ YL +LI+SL  VKD+   LL+FSHD+Y    N ++RSIRFA ++QIF+P+ +QI+   FPG + +DC  D     ++   C NA W D YG+YR  KYTQTKHHW +K + +F+R   L  F    VF+EEDHY+ ED LH+LRLM++ RK     V I+ LGTYEK   F   ++++ +  W+SSFHNMGM+F R++W +IK C+  FC FDDYNWDWSL H+  +C K  FQ M+++ SPR+FHIG+CG+HHKNKVCNP ++  K+ R+L+ N   LYPD++++      R++   PNGGW DLRDR LC   FN+S+
Sbjct:  140 NATAILDAMLAINAEHEILNAQFYNGPITEVIVVQVHDRIGYLYLLIDSLRRVKDVEDCLLVFSHDVYSSAINALVRSIRFARVLQIFYPYIKQIYFDRFPGNDPRDCPRDAGLYRARQLGCNNAEWPDKYGHYRESKYTQTKHHWLYKMNRLFERSPVLANFTGQFVFLEEDHYVVEDLLHMLRLMVAARKDLSQNVEILTLGTYEKKPDFTRNTAQVVVDKWVSSFHNMGMSFSRAIWDQIKPCLPAFCSFDDYNWDWSLNHILTKCTKRPFQTMVIRISPRVFHIGQCGVHHKNKVCNPALLSSKLDRMLANNIAKLYPDQLMFNAKLKIRARALKPNGGWGDLRDRRLCSSFFNSSE 500
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. TrEMBL
Match: A0A1I8HL31 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 370.548 bits (950), Expect = 2.050e-120
Identity = 174/386 (45.08%), Postives = 243/386 (62.95%), Query Frame = 2 
Query:  371 NASNLLRLLHQINSEQIILNLDLFPNP-EFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQ-------------------------IFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNP-SSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQ 1447
            NA+ +L  +  IN+E  ILN   +  P    IV+Q+H+R+ YL +LI+SL  VKD+   LL+FSHD+Y    N ++RSIRFA ++Q                         IF+P+ +QI+   FPG + +DC  D     ++   C NA W D YG+YR  KYTQTKHHW +K + +F+R   L  F    VF+EEDHY+ ED LH+LRLM++ RK     V I+ LGTYEK   F   ++++ +  W+SSFHNMGM+F R++W +IK C+  FC FDDYNWDWSL H+  +C K  FQ M+++ SPR+FHIG+CG+HHKNKVCNP ++  K+ R+L+ N   LYPD++++      R++   PNGGW DLRDR LC   FN+S+
Sbjct:   35 NATAILDAMLAINAEHEILNAQFYNGPITEVIVVQVHDRIGYLYLLIDSLRRVKDVEDCLLVFSHDVYSSAINALVRSIRFARVLQQLLFILIIDQLPKLQSLVTLNSPLQIFYPYTKQIYFDRFPGNDPRDCPRDAGLYRARQLGCNNAEWPDKYGHYRESKYTQTKHHWLYKMNRLFERSPVLANFTGQFVFLEEDHYVVEDLLHMLRLMVAARKDLSQNVEILTLGTYEKKPDFTRNTAQVVVDKWVSSFHNMGMSFSRAIWDQIKPCLPAFCSFDDYNWDWSLNHILTKCTKRPFQTMVIRISPRVFHIGQCGVHHKNKVCNPALLSSKLDRMLANNIAKLYPDQLMFNAKLKIRARALKPNGGWGDLRDRRLCSSFFNSSE 420
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. TrEMBL
Match: R7V5T3 (Uncharacterized protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_23404 PE=4 SV=1)

HSP 1 Score: 359.377 bits (921), Expect = 4.444e-117
Identity = 168/349 (48.14%), Postives = 233/349 (66.76%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLDLF----PNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNP-SSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLC 1423
            L+ Q N +Q ILNLD +     +    IV+Q+HNR++YL  LI+SL   +DI  +LLIFSHD +  E N++I +I F  ++QIF+PF  QI  +EFPG + KDC  D+K+  +  +NC NA + D YG+YR  KY QTKHHW WK   VFD +     F  P++FIEEDHY+ EDF+ VLR M   ++K  P  SI  +G+YEK  ++ P +S++ I  W+SS HNMGM+F++ VW KIK C + FC FDDYNWDWSL ++   C+  + +VM+ K SPRI+HIGECG+HHK K C+P   +++I++LLSQN+Q+L+P  +   G     ++   PNGGW DLRD  LC
Sbjct:    5 LVRQANHQQRILNLDKYELGASDSTVVIVVQVHNRVDYLHPLIDSLRKARDIENTLLIFSHDFFSDEINQVISTIDFCLVMQIFYPFAIQIHPTEFPGESPKDCPRDIKQGEAIKRNCVNAKYPDKYGHYREAKYCQTKHHWFWKLSHVFDHMNVTSSFTGPMLFIEEDHYMVEDFIPVLRKMYRLKEKSCPDCSIFTIGSYEKKPAYGPLASRVDITNWVSSKHNMGMSFNKEVWVKIKSCAQAFCAFDDYNWDWSLHNIGMTCIPNKLKVMVSK-SPRIYHIGECGVHHKGKNCDPAQKVHQIEQLLSQNKQWLFPTALTVAGYPRAPARMPKPNGGWGDLRDHQLC 352
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Cavefish
Match: mgat2 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Source:NCBI gene;Acc:103024773])

HSP 1 Score: 317.005 bits (811), Expect = 1.306e-102
Identity = 152/353 (43.06%), Postives = 221/353 (62.61%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLDLFPN----PEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIY---MGATTTRSKEFH-PNGGWSDLRDRNLCK 1426
            ++ QIN +Q + N D F +     +  IV+Q+HNR EYL +L+ SL   K I   LLIFSHD +  E N+++ SI F  ++QIFFPF+ Q++  EFPG + +DC  D+ KK++    C NA + DS+G+YR  K++QTKHHW WK HFV+DR+R L+  +  ++ IEEDHYL  DF HVL+LM S ++ + P   I+ LG+Y  +   + +++++  AW S+ HNMGM  +R  + K+ KC   FC +DDYNWDWSLQH+   CL   ++VM +  +PRIFH G+CG+HHK   C P     KI+ +L  +   L+P  ++    + A+       H  NGGW D+RD  LCK
Sbjct:   91 IVFQINFDQTLRNEDKFGSVRQKDDLVIVVQVHNRPEYLRLLVESLRKAKGIENVLLIFSHDFWSQEINQVVSSIDFCLVLQIFFPFSIQLYPHEFPGNDPRDCPRDISKKDALKLGCLNAEYPDSFGHYREAKFSQTKHHWWWKLHFVWDRVRVLKDHKGLVLLIEEDHYLAPDFYHVLKLMSSLKRDQCPDCDILSLGSYGHIGYASKANRVEAKAWKSTEHNMGMAMNRETYEKLIKCTDNFCTYDDYNWDWSLQHLTVACLPTFWKVM-VSEAPRIFHAGDCGMHHKKSTCMPNTQKTKIENILQSSSNQLFPKTLLITKRLPASGAGGVAPHVKNGGWGDIRDHELCK 442
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Sea Lamprey
Match: si:ch73-91k6.2 (si:ch73-91k6.2 [Source:ZFIN;Acc:ZDB-GENE-050208-270])

HSP 1 Score: 267.7 bits (683), Expect = 2.203e-84
Identity = 142/370 (38.38%), Postives = 211/370 (57.03%), Query Frame = 2 
Query:  338 SSIFNRNIELMNASNLLR--LLHQINSEQIILNLDLFPNPE---FAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSF-NPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHP-NGGWSDLRDRNLCK 1426
            S+ F+  I + N S  L+  + HQ N  Q I N +    P      I++Q+HNR ++L +L+ SL   + I + LL+ SHD    E N+++R+I F  ++QIFFPF+ QI+ +EFPG +  DC  D+  + ++   C NA + D Y +YR   +TQTKHHW WK HFV+DRL  +      ++ IEEDHYL  DF  VL+ M   +K++ P   I+ LG+Y+ V +F   S K++   W SS HNMGM   R  +  +  C +EFC +DDYNWDW+LQH+   CL    + ++L+ +PR+ H G CG+HH+   C+      ++   +S N  +LYP  +  +          H  NGGW D RD  LC+
Sbjct:   69 SNFFDVKIGVKNTSKELKKYIYHQ-NFYQFIRNSEKIKAPAKDGLVILVQVHNRPQHLKLLVESLRKAQRIEEVLLVLSHDYISEEVNEVVRTIDFCRVLQIFFPFSLQIYPNEFPGQDPNDCPRDISLEEARRLKCNNAEFPDKYAHYREAVFTQTKHHWWWKLHFVWDRLNAINAHDGLVLLIEEDHYLLPDFYVVLQKMWDMQKRECPDCDILSLGSYDMVDNFEGKSDKLEGLPWQSSRHNMGMAIGRLTYKHLLACTQEFCTYDDYNWDWTLQHLTVACLPHPLKALVLQ-APRVRHTGGCGMHHQGP-CDAEGEARRLAEHVSANVPHLYPTTLQILQMHYVSPINPHTVNGGWGDKRDHALCQ 435
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Sea Lamprey
Match: si:ch73-91k6.2 (si:ch73-91k6.2 [Source:ZFIN;Acc:ZDB-GENE-050208-270])

HSP 1 Score: 231.876 bits (590), Expect = 3.010e-71
Identity = 121/363 (33.33%), Postives = 184/363 (50.69%), Query Frame = 2 
Query:  374 ASNLLRLLHQINSEQIILN----LDLFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEK--------VHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHI-GECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLC 1423
            A+ + RL+ ++N+EQ   N    L   P     +V+Q+H+R  +L +L+ SL  V+ I  +LLIFSHD    E + ++R +RF  ++QIF+PF+ Q+  + FPG + +DC  +   + +Q   C NA   D +G+YR   +TQ KHHW WK HFV+  LR L     P++ +EEDH++  D L +L  M    +       ++ LG              +   +  + +  W SS HN GM   RS + K+ +C + FC  DDYNWDWS+Q     C+     ++L    PR+ H  G CG+HH+   C          R+L Q    L+P  +         +     NGGW+D RD  LC
Sbjct:   28 AAAIRRLVLRMNAEQRPRNAGRALAWRPGGPV-VVVQVHSRAAHLRLLVESLGAVRGIETALLIFSHDEVSEEVDAVVRGVRFCQVLQIFYPFSAQLHAASFPGPDPRDCPRNASPREAQRLGCTNAATPDRFGHYREAAFTQAKHHWWWKLHFVWRGLRMLRGHPGPVLLLEEDHFVLPDALALLASMGDVLRVNCADCDLLSLGVLGPAVGAAAAAAVAAAGTEAVDVAPWHSSRHNTGMAMMRSGYEKLARCARAFCTCDDYNWDWSVQLASVDCVPRGAMLVLATRLPRVLHTGGGCGMHHRRDSCGVQQERAHAMRVLRQLEGALFPAALSIRSKNEKGAIRPRLNGGWADPRDWALC 389
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Medaka
Match: mgat2 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Source:NCBI gene;Acc:101165022])

HSP 1 Score: 317.39 bits (812), Expect = 4.351e-103
Identity = 147/353 (41.64%), Postives = 225/353 (63.74%), Query Frame = 2 
Query:  392 LLHQINSEQIILNLDLFPNP----EFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIY---MGATTTRSKEFH-PNGGWSDLRDRNLCK 1426
            ++ Q+N +Q I N   F       +  +V+Q+HNR +YL +L++SL   + +   LLIFSHD + PE NK++ S+ F  ++QIFFPF+ Q++  EFPG + KDC  D+ KK++    C NA + DS+G+YR  K++QTKHHW WK HFV+DR+ +L+  +  ++ IEEDHYL+ DF+H+L+LM + ++++ P   I+ LG+Y  +   + ++K+++ AW S+ HNMGM  +R  + K+ KC   FC +DDYNWDWSLQH+   CL   ++VM +  +PRIFH G+CG+HHK   C P     KI+ +L  +   L+P  ++    + A+       H  NGGW D+RD  LCK
Sbjct:   76 IVFQLNFDQTIRNEKKFKTARQKDDLVVVVQVHNRPDYLKLLVDSLRKARGVDSILLIFSHDYWSPEINKVVASVDFCQVLQIFFPFSIQLYPHEFPGNDPKDCPRDIPKKDALKLGCINAEFPDSFGHYREAKFSQTKHHWWWKLHFVWDRVHYLKDHKGLVLLIEEDHYLSPDFIHLLKLMTALKREQCPDCDILSLGSYSHISYSSKANKVEVKAWKSTEHNMGMALNRETYQKLLKCTDTFCTYDDYNWDWSLQHLTVNCLPTYWKVM-VSEAPRIFHAGDCGMHHKKATCMPVTQKTKIENILQSSGNQLFPKYLLITKRLPASGAGGVAPHVKNGGWGDIRDHELCK 427
[Close]
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Planmine SMEST
Match: SMESG000047050.1 (SMESG000047050.1)

HSP 1 Score: 1014.22 bits (2621), Expect = 0.000e+0
Identity = 495/496 (99.80%), Postives = 495/496 (99.80%), Query Frame = 2 
Query:   29 MRPQRILNCILWIFFIGISLHIILYLLIPIFFNDENFENNYLGVNRDVKELKTDQINLRLSLIDKLRTQFIEIFNFNPSPNEGPVLLERKRNRNFKQNNTYPISSIFNRNIELMNASNLLRLLHQINSEQIILNLDLFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQLLDNLTSLESSNGHLSVLESLLI 1516
            MRPQRILNCILWIFFIGISLHIILYLLIPIFFNDENFENNYLGVNRDVKELKTDQINLRLSLIDKLRTQFIEIFNFNPSPNEGPVLLERKRNRNFKQNNTYPISSIFNRNIELMNASNLLRLLHQINSEQIILNLDLFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQLLDNLTSLESSNGHLSVLES LI
Sbjct:    1 MRPQRILNCILWIFFIGISLHIILYLLIPIFFNDENFENNYLGVNRDVKELKTDQINLRLSLIDKLRTQFIEIFNFNPSPNEGPVLLERKRNRNFKQNNTYPISSIFNRNIELMNASNLLRLLHQINSEQIILNLDLFPNPEFAIVIQIHNRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQIFFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNYKYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLMLSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRSVWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHIGECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEFHPNGGWSDLRDRNLCKVLFNNSQLLDNLTSLESSNGHLSVLESFLI 496
The following BLAST results are available for this feature:
BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MGAT22.543e-9941.14alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gly-204.586e-8836.30GLYcosylation related [Source:UniProtKB/TrEMBL;Ac... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Mgat26.363e-10543.02gene:FBgn0039738 transcript:FBtr0100418[more]
Mgat28.216e-10240.90gene:FBgn0039738 transcript:FBtr0334705[more]
Mgat22.804e-4941.12gene:FBgn0039738 transcript:FBtr0085602[more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mgat27.237e-10441.16alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
si:ch73-91k6.21.131e-9037.30si:ch73-91k6.2 [Source:ZFIN;Acc:ZDB-GENE-050208-27... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mgat26.042e-9641.67mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acet... [more]
ENSXETT00000014814.19.184e-5529.90alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mgat23.609e-10241.53mannoside acetylglucosaminyltransferase 2 [Source:... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q09326|MGAT2_RAT1.642e-10342.37Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
sp|Q921V5|MGAT2_MOUSE2.525e-10141.53Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
sp|O19071|MGAT2_PIG2.945e-10140.97Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
sp|Q10469|MGAT2_HUMAN1.221e-9841.14Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
sp|Q9FT88|MGAT2_ARATH3.139e-4632.20Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FKN72.239e-12548.20Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8JGE42.966e-12548.20Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FTV09.330e-12548.20Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HL312.050e-12045.08Uncharacterized protein OS=Macrostomum lignano OX=... [more]
R7V5T34.444e-11748.14Uncharacterized protein (Fragment) OS=Capitella te... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mgat21.306e-10243.06alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:ch73-91k6.22.203e-8438.38si:ch73-91k6.2 [Source:ZFIN;Acc:ZDB-GENE-050208-27... [more]
si:ch73-91k6.23.010e-7133.33si:ch73-91k6.2 [Source:ZFIN;Acc:ZDB-GENE-050208-27... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mgat24.351e-10341.64alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglu... [more]
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BLAST of Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000047050.10.000e+099.80SMESG000047050.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020599 ID=SMED30020599|Name=Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=1612bp
TATATAAATCTTATATGATGTATTGTAAATGAGGCCCCAGAGGATATTAA
ATTGTATATTGTGGATATTTTTTATCGGAATCAGTTTACATATCATATTG
TACTTACTTATTCCGATTTTCTTTAATGATGAAAATTTTGAAAATAATTA
TTTGGGTGTTAATCGTGATGTTAAAGAACTTAAAACTGATCAAATAAATC
TGCGATTGTCATTAATAGACAAATTACGTACACAGTTCATTGAAATTTTC
AATTTTAATCCCAGTCCAAACGAAGGTCCGGTGTTGCTGGAAAGGAAGAG
AAATAGAAATTTCAAACAAAATAATACATATCCCATCTCATCAATATTCA
ATAGAAATATAGAATTAATGAATGCTTCCAATTTATTACGTTTGCTACAC
CAAATTAATTCAGAACAGATTATTCTCAATCTTGATCTTTTTCCAAACCC
AGAATTCGCAATAGTCATACAAATTCATAACAGATTGGAGTATCTAACAA
TCCTTATCAATTCTTTAATAAATGTCAAAGATATCCACAAAAGTCTTTTA
ATATTCAGTCATGATATGTATTTTCCAGAATTTAATAAAATTATCCGGTC
AATTAGATTTGCTCCTCTTGTACAAATATTCTTTCCTTTTAATCAACAGA
TTTTCAAATCAGAATTTCCGGGCTACAACAGTAAAGATTGTGATGTGGAT
ATGAAGAAAAAGAATTCACAACTAAAGAACTGTTTCAATGCTAACTGGAG
CGATTCTTATGGAAATTATAGAAATTACAAATACACACAGACTAAGCATC
ATTGGAGTTGGAAAGCTCATTTTGTATTTGATCGTTTACGGTTTCTGGAA
ATCTTCCAAAACCCGATTGTATTTATTGAAGAGGATCATTATTTAACGGA
AGATTTTCTTCACGTATTACGGTTGATGCTGTCAGAAAGAAAAAAAAAGT
ATCCCCAAGTTTCCATAATATGTCTAGGAACATATGAAAAAGTTCATTCT
TTCAATCCTTCATCAAAGATACAAATCGGCGCATGGCTGTCCAGCTTTCA
TAACATGGGTATGACTTTCGACAGGTCTGTGTGGACGAAAATTAAAAAGT
GTGTCAAAGAGTTTTGTGAGTTTGACGACTACAATTGGGACTGGAGCTTA
CAGCACGTTCATAAGCGATGTTTGAAAGAAGAATTTCAAGTCATGCTTTT
GAAAAACTCACCGAGAATTTTTCATATTGGAGAATGTGGAATCCATCACA
AAAACAAAGTCTGTAATCCATATGTGGTGCTTTATAAAATTCAAAGATTA
TTATCACAAAATAGACAATATCTTTATCCAGATGAAATGATATATATGGG
AGCGACAACTACTCGTTCAAAGGAGTTCCATCCAAACGGCGGATGGTCAG
ATCTTCGGGACAGAAACCTTTGTAAAGTTTTATTTAACAATTCTCAGTTA
TTGGACAATTTAACAAGTTTGGAGTCATCAAATGGTCATTTATCTGTTCT
GGAATCCCTTTTGATATAGTAATATCTTTTCAAGTGTTTATTTATATATA
GGTGATGATTTCAATATTTTTATATTTATTTTTAGTATTTGTACGAATCC
ATTTTATTTTTA
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protein sequence of SMED30020599-orf-1

>SMED30020599-orf-1 ID=SMED30020599-orf-1|Name=SMED30020599-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=497bp
MRPQRILNCILWIFFIGISLHIILYLLIPIFFNDENFENNYLGVNRDVKE
LKTDQINLRLSLIDKLRTQFIEIFNFNPSPNEGPVLLERKRNRNFKQNNT
YPISSIFNRNIELMNASNLLRLLHQINSEQIILNLDLFPNPEFAIVIQIH
NRLEYLTILINSLINVKDIHKSLLIFSHDMYFPEFNKIIRSIRFAPLVQI
FFPFNQQIFKSEFPGYNSKDCDVDMKKKNSQLKNCFNANWSDSYGNYRNY
KYTQTKHHWSWKAHFVFDRLRFLEIFQNPIVFIEEDHYLTEDFLHVLRLM
LSERKKKYPQVSIICLGTYEKVHSFNPSSKIQIGAWLSSFHNMGMTFDRS
VWTKIKKCVKEFCEFDDYNWDWSLQHVHKRCLKEEFQVMLLKNSPRIFHI
GECGIHHKNKVCNPYVVLYKIQRLLSQNRQYLYPDEMIYMGATTTRSKEF
HPNGGWSDLRDRNLCKVLFNNSQLLDNLTSLESSNGHLSVLESLLI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005795Golgi stack
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0003116parenchymal cell
Vocabulary: molecular function
TermDefinition
GO:0008455alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
Vocabulary: biological process
TermDefinition
GO:0009312oligosaccharide biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR029044Nucleotide-diphossugar_trans
IPR007754GlcNAc_II
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007754N-acetylglucosaminyltransferase IIPFAMPF05060MGAT2coord: 123..465
e-value: 7.4E-130
score: 433.1
IPR007754N-acetylglucosaminyltransferase IIPANTHERPTHR12871BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE IIcoord: 105..467
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILYSSF53448Nucleotide-diphospho-sugar transferasescoord: 143..404
NoneNo IPR availableTMHMMTMhelixcoord: 7..29